CN208104443U - A kind of upper magnetic stirring apparatus for cell culture - Google Patents
A kind of upper magnetic stirring apparatus for cell culture Download PDFInfo
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- CN208104443U CN208104443U CN201820334351.XU CN201820334351U CN208104443U CN 208104443 U CN208104443 U CN 208104443U CN 201820334351 U CN201820334351 U CN 201820334351U CN 208104443 U CN208104443 U CN 208104443U
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- bearing
- central axis
- isolation cover
- cell culture
- speed reducer
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Abstract
The utility model discloses a kind of upper magnetic stirring apparatus for cell culture, it includes central axis（1）, the mounting plate that is sequentially arranged from lower to upper（2）, motor support base（3）, thermal insulation board（4）, speed reducer connecting plate（5）, speed reducer（6）And motor（7）, the motor（7）With speed reducer（6）Connection, speed reducer（6）Output shaft（8）On be connected with coupling sleeve（9）, coupling sleeve（9）Inner wall on be provided with outer steel（10）；Central axis（1）It is inside provided with positioned at isolation cover（14）Interior L shape channel（18）, L shape channel（18）One end run through central axis（1）Top surface, L shape channel（18）The other end run through central axis（1）Cylinder, isolation cover（14）CIP/SIP union is provided on cylinder（19）.The utility model has the beneficial effects that：Compact-sized, easy to assemble, low energy consumption, can be cleaned and be sterilized online, be cleaned and be sterilized thorough and high-efficient at zero leakage.
Description
Technical field
The utility model relates to be bio-pharmaceutical industry cell suspension cultures, microcarrier culture, sheet culture, especially one
Kind is used for the upper magnetic stirring apparatus of cell culture.
Background technique
Cell culture is to develop a kind of very rapid experimental technique in modern biotechnology science, in bio-pharmaceutical industry,
Cell culture already belongs to process upstream exploitation, and market prospects are very big, and in China, which is still in the junior stage, Hen Duosuo
The various kinds of equipment needed mostly uses greatly external import at present, not only involves great expense, but also the supply of standby redundancy is also greatly led
System.In cell culture process, the requirement for stirring is particularly harsh, not only to meet air-tightness requirement, accomplishes entirely without letting out
Leakage will also meet the mixing of the various nutrients needed for cell under low sheraing, slow-speed of revolution operation, guarantee that gas uniformly divides in tank
Cloth improves cell dissolved oxygen etc., and cell culture process is all using fining culture mostly, the case where meeting using function
Under, it is desirable that mixing plant is small and exquisite as far as possible, and beautiful, this significantly limits the apparent size of equipment, installation dimension.Therefore for this
The novel culture process of kind, traditional mechanical stirring or magnetic agitation have been unable to meet requirement.Magnetic agitation is used at present
Axis is used for the mixing plant of bio-pharmaceuticals, but carries out on-line cleaning to central axis, interior magnet steel, isolation cover inner wall, bearing and go out
When bacterium, it to be passed through cleaning solution in the middle part of isolation cover, however enters the cleaning solution in isolation cover or the most flow down of steam,
Can only carry out on-line cleaning and sterilizing to the bearing being located at below cleaning solution inlet port, and can not be to being located above inlet port in
Magnet steel, isolation cover inner wall carry out on-line cleaning and sterilizing, the mixing plant are difficult to reach the sterility requirements of bio-pharmaceuticals equipment, deposit
In cleaning and sterilizing is not thorough and the defect of low efficiency.
Utility model content
The shortcomings that the purpose of the utility model is to overcome the prior arts provides a kind of compact-sized, compact structure, readily removable
Dress, zero leakage, can be cleaned and be sterilized online, be cleaned and be sterilized thorough and high-efficient for cell culture at low energy consumption
Upper magnetic stirring apparatus.
The purpose of this utility model is achieved through the following technical solutions：A kind of upper magnetic agitation dress for cell culture
It sets, mounting plate, motor support base, thermal insulation board, the speed reducer connecting plate, speed reducer that it includes central axis, is sequentially arranged from lower to upper
And motor, the motor are connect with speed reducer, and coupling sleeve is connected on the output shaft of speed reducer, coupling sleeve is inserted into motor support base
It is interior, outer steel is provided on the inner wall of coupling sleeve, bearing block is provided in the mounting plate, be equipped in bearing block lower bearing and
Thrust ceramic bearing, lower bearing are located at the surface of thrust ceramic bearing, are provided with isolation cover at the top of bearing block, isolation cover is stretched
Enter in coupling sleeve and its is top closed, upper bearing (metal) is installed in isolation cover；The central axis sequentially run through thrust ceramic bearing,
Lower bearing and upper bearing (metal) are arranged, and interior magnet steel is provided on central axis, and interior magnet steel is set in isolation cover and is located at below upper bearing (metal),
The lower end of central axis is connected with impeller, and impeller is located at below bearing block；The L in isolation cover is provided in the central axis
The top surface of central axis is run through in shape channel, the one end in L shape channel, and the other end in L shape channel runs through the cylinder of central axis, it is described every
From being provided with CIP/SIP union on cover cylinder.
The interior magnet steel and outer steel are in same level height.
The upper bearing (metal) and lower bearing are deep groove ball bearing.
The lower end of the central axis is connected with agitating shaft through shaft coupling.
The impeller is installed on agitating shaft.
O-ring seal is provided between the bearing block and isolation cover, between bearing block and mounting plate.
The utility model has the following advantages that：
（1）The utility model transmits power by magnetic coupling, is separated outer magnetic and interior magnetic using isolation cover, to realize
Power is transmitted without friction, also, isolation cover uses static seal mode, so that effectively preventing tradition machinery sealing dynamic friction has object
Matter falls off, and generates the risk polluted to material is stirred in tank.
（2）Completely new CIP/SIP theory, cleaning solution or pure steam are entered by interface below isolation cover, agitated center
Axis is directly entered above isolation cover, and a kind of top-down spray mode is formed above isolation cover, is revolutionized existing clear
It washes and sterilizes mode, accomplished cleaning, all standing of sterilizing truly, while cleaning and sterilization effect is more thorough.
（3）The design of conventional magnetic stirring structure is optimized, keeps its structure simpler, ingenious, magnetic force is furthermore achieved
The development need of stirring fining, miniaturization, product practicability, spreadability further increase.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is the scheme of installation of center axis and isolation cover and bearing block；
In figure, 1- central axis, 2- mounting plate, 3- motor support base, 4- thermal insulation board, 5- speed reducer connecting plate, 6- speed reducer, 7-
Motor, 8- output shaft, 9- coupling sleeve, 10- outer steel, 11- bearing block, 12- lower bearing, 13- thrust ceramic bearing, 14- isolation
It covers, 15- upper bearing (metal), magnet steel in 16-, 17- impeller, 18-L shape channel, 19-CIP/SIP union, 20- shaft coupling, 21- stirring
Axis, 22- O-ring seal.
Specific embodiment
The utility model is further described with reference to the accompanying drawing, the protection scope of the utility model be not limited to
It is lower described：
As shown in Figure 1 and 2, a kind of upper magnetic stirring apparatus for cell culture, it includes central axis 1, suitable from lower to upper
Mounting plate 2, motor support base 3, thermal insulation board 4, speed reducer connecting plate 5, speed reducer 6 and the motor 7 of secondary setting, the motor 7 with subtract
Fast machine 6 connects, and is connected with coupling sleeve 9 on the output shaft 8 of speed reducer 6, coupling sleeve 9 is inserted into motor support base 3, coupling sleeve 9
It is provided with outer steel 10 on inner wall, bearing block 11 is provided in the mounting plate 2, lower bearing 12 is installed in bearing block 11 and stops
Ceramic bearing 13 is pushed away, lower bearing 12 is located at the surface of thrust ceramic bearing 13, and the top of bearing block 11 is provided with isolation cover 14,
Isolation cover 14 is inserted into coupling sleeve 9 and its is top closed, and upper bearing (metal) 15 is equipped in isolation cover 14；The central axis 1 is sequentially
It is arranged through thrust ceramic bearing 13, lower bearing 12 and upper bearing (metal) 15, is provided with interior magnet steel 16 on central axis 1, interior magnet steel 16 is set
It is placed in isolation cover 14 and is located at 15 lower section of upper bearing (metal), the lower end of central axis 1 is connected with impeller 17, and impeller 17 is located at bearing block
11 lower sections；The L shape channel 18 in isolation cover 14 is provided in the central axis 1, central axis is run through in the one end in L shape channel 18
1 top surface, the other end in L shape channel 18 run through the cylinder of central axis 1, are provided with CIP/SIP on 14 cylinder of isolation cover
Union 19.
The interior magnet steel 16 and outer steel 10 are in same level height, and interior magnet steel 16 and outer steel 10 are all made of and are resistant to
The high temperature magnet of 180 DEG C of high temperature guarantees to use under 0 ~ 130 DEG C of operating condition, and magnetic energy product is consistent with magnetic force coercivity, and magnetic force transmits not
It is affected by temperature and causes damages, magnetic agitation is reliable for operation.The upper bearing (metal) 15 and lower bearing 12 are deep groove ball bearing.Institute
The lower end for stating central axis 1 is connected with agitating shaft 21 through shaft coupling 20, and impeller 17 is installed on agitating shaft 21.The bearing block 11
Between isolation cover 14, O-ring seal 22 is provided between bearing block 11 and mounting plate 2, O-ring seal 22 is high temperature resistant energy
The perfluoroelastomer sealing ring of strong, the resistance to alkali liquid corrosion of power.
The course of work of the utility model is as follows：Mounting plate 2 is fixedly installed in the top of bio-pharmaceuticals equipment through screw,
When being stirred to bio-pharmaceuticals equipment inner cell suspension, start motor 7, the torque of motor 7 band after the deceleration of speed reducer 6
Dynamic output shaft 8 rotates, and output shaft 8 drives the outer steel 10 on coupling sleeve 9 to make rotating motion, in outer steel 10 and interior magnet steel 16
Magnet steel 16 makes rotating motion in driving under coupling, is further driven to central axis 1 and makes rotating motion, and it is outstanding that impeller 17 stirs cell
Supernatant liquid.Since isolation cover 14 separates outer steel 10 and interior magnet steel 16, power is transmitted without friction to realize, and isolation cover 14 is adopted
With static seal mode, there is substance to fall off to effectively prevent tradition machinery sealing dynamic friction, generate dirt to material is stirred in tank
The risk of dye.
When on-line cleaning to be carried out and sterilizing, closes motor 7 and be passed through cleaning solution into CIP/SIP union 19, clearly
Washing lotion enters through the horizontal channel in L shape channel 18, finally sprays from the vertical channel in L shape channel 18, rectangular on upper bearing (metal) 15
At a kind of top-down spray mode, cleaning solution enters the seam interior magnet steel 16 and isolation cover 14 again after upper bearing (metal) 15
In gap, to realize the on-line cleaning and sterilizing to isolation cover inner wall and interior magnet steel；Then spray and under cleaning solution it is suitable
It is secondary to pass through lower bearing 12 and thrust ceramic bearing 13, to realize the on-line cleaning and sterilizing to bearing, prevent because of stirring
Bring pollution risk has reached the sterility requirements of bio-pharmaceuticals equipment, compares conventional clean and sterilization method, realizes without dead
The thorough cleaning and sterilizing at angle.
Claims (6)
1. a kind of upper magnetic stirring apparatus for cell culture, it is characterised in that：It includes central axis（1）, it is suitable from lower to upper
The mounting plate of secondary setting（2）, motor support base（3）, thermal insulation board（4）, speed reducer connecting plate（5）, speed reducer（6）And motor（7）, institute
State motor（7）With speed reducer（6）Connection, speed reducer（6）Output shaft（8）On be connected with coupling sleeve（9）, coupling sleeve（9）It protrudes into
In motor support base（3）It is interior, coupling sleeve（9）Inner wall on be provided with outer steel（10）, the mounting plate（2）Inside it is provided with bearing block
（11）, bearing block（11）Lower bearing is inside installed（12）With thrust ceramic bearing（13）, lower bearing（12）Positioned at thrust ceramic shaft
It holds（13）Surface, bearing block（11）Top be provided with isolation cover（14）, isolation cover（14）It is inserted into coupling sleeve（9）In and
Its is top closed, isolation cover（14）Upper bearing (metal) is inside installed（15）；The central axis（1）Sequentially run through thrust ceramic bearing
（13）, lower bearing（12）And upper bearing (metal)（15）Setting, central axis（1）On be provided with interior magnet steel（16）, interior magnet steel（16）It is set to
Isolation cover（14）It is interior and be located at upper bearing (metal)（15）Lower section, central axis（1）Lower end be connected with impeller（17）, impeller（17）It is located at
Bearing block（11）Lower section；The central axis（1）It is inside provided with positioned at isolation cover（14）Interior L shape channel（18）, L shape channel（18）
One end run through central axis（1）Top surface, L shape channel（18）The other end run through central axis（1）Cylinder, the isolation cover
（14）CIP/SIP union is provided on cylinder（19）.
2. a kind of upper magnetic stirring apparatus for cell culture according to claim 1, it is characterised in that：The interior magnetic
Steel（16）And outer steel（10）In same level height.
3. a kind of upper magnetic stirring apparatus for cell culture according to claim 1, it is characterised in that：The upper axis
It holds（15）And lower bearing（12）It is deep groove ball bearing.
4. a kind of upper magnetic stirring apparatus for cell culture according to claim 1, it is characterised in that：The center
Axis（1）Lower end through shaft coupling（20）It is connected with agitating shaft（21）.
5. a kind of upper magnetic stirring apparatus for cell culture according to claim 1, it is characterised in that：The impeller
（17）It is installed on agitating shaft（21）On.
6. a kind of upper magnetic stirring apparatus for cell culture according to claim 1, it is characterised in that：The bearing
Seat（11）With isolation cover（14）Between, bearing block（11）With mounting plate（2）Between be provided with O-ring seal（22）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820334351.XU CN208104443U (en) | 2018-03-12 | 2018-03-12 | A kind of upper magnetic stirring apparatus for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820334351.XU CN208104443U (en) | 2018-03-12 | 2018-03-12 | A kind of upper magnetic stirring apparatus for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208104443U true CN208104443U (en) | 2018-11-16 |
Family
ID=64126218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820334351.XU Active CN208104443U (en) | 2018-03-12 | 2018-03-12 | A kind of upper magnetic stirring apparatus for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208104443U (en) |
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2018
- 2018-03-12 CN CN201820334351.XU patent/CN208104443U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191216 Address after: 610000 Chengdu economic and Technological Development Zone (Longquanyi District), Chengdu, Sichuan Province, No. 669 South Road Patentee after: Chengdu Yingde Bio-Pharmaceutical Co., Ltd. Address before: 610000 No. 1 consulate Road, Wuhou District, Chengdu, Sichuan (No. 2, two building, Nan Yi Building) Patentee before: CHENGDU YINGDE BIO-MEDICAL EQUIPMENT TECHNOLOGY CO., LTD. |