CN208055329U - A kind of Ke Liben series bacillus separation of fermentative broth device - Google Patents
A kind of Ke Liben series bacillus separation of fermentative broth device Download PDFInfo
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- CN208055329U CN208055329U CN201820337741.2U CN201820337741U CN208055329U CN 208055329 U CN208055329 U CN 208055329U CN 201820337741 U CN201820337741 U CN 201820337741U CN 208055329 U CN208055329 U CN 208055329U
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Abstract
The utility model discloses a kind of Ke Liben series bacillus separation of fermentative broth devices, it includes centrifuging room, the heavy sugared room of ethyl alcohol, by-pass filtration cylinder, level-one cylinder, fluid reservoir, it is sirred and separated room, secondary filtration cylinder and the second-order separation cylinder, it centrifuges and is provided with supernatant outlet and concentrated solution outlet on room, supernatant outlet is connected with the entrance for being sirred and separated room, concentrated solution outlet is connected with the heavy sugared room of ethyl alcohol, the heavy sugared room of ethyl alcohol is connected with the entrance of by-pass filtration cylinder, level-one cylinder and by-pass filtration cylinder, fluid reservoir is connected, the slag-drip opening of level-one cylinder is connected with the entrance for being sirred and separated room, the outlet for being sirred and separated room is connected with the entrance of secondary filtration cylinder, secondary filtration cylinder and the second-order separation cylinder, fluid reservoir is connected.The waste residue that generates is stirred separation after carrying out first separation to zymotic fluid in the utility model, avoids the problem that in waste residue that the aggregation of zymotic fluid active constituent causes separation to be not thorough and zymotic fluid active constituent detaches to be not thorough leads to inferior separating effect.
Description
Technical field
The utility model is related to separation of fermentative broth technical fields, more particularly to a kind of Ke Liben series bacillus zymotic fluid
Separator.
Background technology
Ke Liben series bacillus (Hypocreavirens) is a kind of heavy responsibilities of government inhibiting a variety of Soil-born plant pathogenic fungis
Raw bacterium, bacterium colony growth is fast, 7~9.5cm of diameter after culture 4 days, aerial hyphae frizzle shape, white or grey.Conidium is rich
Richness is bordering on open and flat shape and is covered in tablet.Main mechanism of the Ke Liben series bacillus as biocontrol of plant disease, has got over
Paid attention to by various countries phytopathologist to get over.Important factor of the Hyperparasite as biological control, for preventing phytopathy
Evil plays an important roll.
In the prior art, the report about Ke Liben series bacillus zymotechniques is less, only Patent No. CN
The patent of invention of 103756931 A describes the related content of discovery and the application about Ke Liben series bacillus, Ke Liben
Series bacillus cultural method and culture medium are only two aspects of activation culture and activation medium to Ke Liben series bacillus
It is on the books, Ke Liben series bacillus separation of fermentative broth is not specifically introduced；Therefore, it is urgent to provide one kind for gram in
The equipment that this series bacillus zymotic fluid efficiently separates.
Utility model content
The purpose of this utility model is that it is in view of the drawbacks of the prior art and insufficient, a kind of simple in structure, separation effect is provided
The good Ke Liben series bacillus separation of fermentative broth devices of fruit.
To achieve the above object, the utility model provides following scheme：The utility model provides a kind of Ke Liben classes bud
Spore bacillus fermentation liquid separating apparatus, including centrifuge room, the heavy sugared room of ethyl alcohol, by-pass filtration cylinder, level-one cylinder, fluid reservoir,
It is sirred and separated room, secondary filtration cylinder and the second-order separation cylinder, supernatant outlet is provided on the centrifugation room and concentrate goes out
Mouthful, the supernatant outlet is connected with the entrance for being sirred and separated room, the concentrated solution outlet and the heavy sugared room of the ethyl alcohol
Entrance be connected, the outlet of the heavy sugared room of the ethyl alcohol is connected with the entrance of the by-pass filtration cylinder, the level-one cylinder
Be both provided with filtering film component in the second-order separation cylinder, on the outer wall of the level-one cylinder and the second-order separation cylinder
It is provided with inlet, slag-drip opening and liquid outlet, the inlet of the level-one cylinder is connected with the outlet of the by-pass filtration cylinder
Logical, the liquid outlet of the level-one cylinder is connected with the fluid reservoir, slag-drip opening and the stirring of the level-one cylinder
The entrance of separation chamber is connected, and the sirring and separating interior is provided with blender, the outlet and described two for being sirred and separated room
The entrance of grade cartridge filter is connected, and the outlet of the secondary filtration cylinder is connected with the inlet of the second-order separation cylinder, described
The liquid outlet of the second-order separation cylinder is connected with the fluid reservoir, and the slag-drip opening of the second-order separation cylinder is connected with the external world.
Preferably, the filtering film component includes at least one filter membrane.
Preferably, the filtering film component includes filter membrane one, filter membrane two, filter membrane three and filter membrane four, the mistake
Filter membrane one, filter membrane two, filter membrane three and filter membrane four filter diameter be sequentially reduced, the level-one cylinder and the two level
Slag-drip opening one, slag-drip opening two, slag-drip opening three and slag-drip opening four are both provided on the outer wall of cylinder.
Preferably, a diameter of 0.08 μm of the filter hole of the filter membrane one, the filter hole of the filter membrane two is a diameter of
0.06 μm, a diameter of 0.04 μm of the filter hole of the filter membrane three, a diameter of 0.02 μm of the filter hole of the filter membrane four.
Preferably, cooling chamber, the heavy sugared room of the ethyl alcohol are provided between the heavy sugared room of the ethyl alcohol and the by-pass filtration cylinder
Spiral coil is provided between the entrance of the by-pass filtration cylinder, the spiral coil is arranged in the cooling chamber.
Preferably, the cooling chamber is connected with a circulating water machine, the inlet and outlet of the circulating water machine
It is connected with the cooling chamber.
Preferably, the spiral coil is metal thin-wall corrugated pipe coil.
Preferably, the spiral coil is connected with a sterile gas supply mechanism, and the sterile gas supply mechanism includes being connected
Desinfection chamber and vacuum pump, the outlet side of the vacuum pump is connected with the spiral coil.
Preferably, the spiral coil is connected with a wiper mechanism, and the wiper mechanism includes the water tank being connected
And water pump, the water outlet of the water pump are connected with the spiral coil.
Preferably, it is provided with a temperature sensor in the spiral coil.
The utility model achieves following advantageous effect compared with the existing technology：
1, room is sirred and separated using setting in Ke Liben series bacillus separation of fermentative broth device provided by the utility model,
And the entrance for being sirred and separated room is connected with supernatant outlet and slag-drip opening, it is sirred and separated outlet and the secondary filtration cylinder of room
The mode that entrance is connected so that zymotic fluid filter after first separation the waste residue of generation and is stirred point in separator
From avoiding in waste residue that the aggregation of zymotic fluid active constituent causes separation to be not thorough and the separation of zymotic fluid active constituent is not thorough causes
The problem of inferior separating effect, meanwhile, it is reused to centrifuging the supernatant generated in room, improves supernatant
Service efficiency.
2, it is used in Ke Liben series bacillus separation of fermentative broth device provided by the utility model and places spiral coil
In the mode of cooling chamber, the fermentation liquid energy in coil pipe is made to be cooled down, prevents from causing since temperature is excessively high during conveying
The problem of strain death.
3, pass through filter membrane one, filter membrane in Ke Liben series bacillus separation of fermentative broth device provided by the utility model
Two, filter membrane three and filter membrane four are filtered for multiple times separation small molecule filtrate, ensure the quality of production of product.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only the utility model
Some embodiments for those of ordinary skill in the art without having to pay creative labor, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 is the overall structure diagram of the utility model Ke Liben series bacillus separation of fermentative broth devices；
Fig. 2 is the structural schematic diagram of the utility model cooling chamber and spiral coil；
Wherein, 1- centrifuges room, 2- by-pass filtrations cylinder, the heavy sugared room of 3- ethyl alcohol, 4- level-ones cylinder, 5- fluid reservoirs, 6-
It is sirred and separated room, 7- secondary filtrations cylinder, 8- the second-order separations cylinder, 9- filter membranes one, 10- filter membranes two, 11- filter membranes three, 12- mistakes
Filter membrane four, 13- cooling chambers, 14- spiral coils, 15- circulating waters machine, 16- desinfection chambers, 17- vacuum pumps, 18- water tanks, 19-
Water pump.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
The purpose of this utility model is to provide a kind of Ke Liben series bacillus simple in structure, good separating effect to ferment
Liquid separating apparatus.
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, below in conjunction with the accompanying drawings and have
Body embodiment is described in further detail the utility model.
As shown in Figs. 1-2, the utility model provides a kind of Ke Liben series bacillus separation of fermentative broth device, including centrifugation
Separation chamber 1, by-pass filtration cylinder 2, level-one cylinder 4, fluid reservoir 5, is sirred and separated room 6,7 and of secondary filtration cylinder at the heavy sugared room 3 of ethyl alcohol
The second-order separation cylinder 8 centrifuges and is provided with supernatant outlet and concentrated solution outlet on room 1, supernatant outlet and sirring and separating room 6
Entrance be connected, the entrance of the heavy sugared room of concentrated solution outlet and ethyl alcohol 3 is connected, the outlet of the heavy sugared room of ethyl alcohol 3 and by-pass filtration
The entrance of cylinder 2 is connected, and filtering film component, level-one cylinder 4 and two are both provided in level-one cylinder 4 and the second-order separation cylinder 8
It is both provided with inlet, slag-drip opening and liquid outlet, the inlet and by-pass filtration of level-one cylinder 4 on the outer wall of grade cylinder 8
The outlet of cylinder 2 is connected, and the liquid outlet of level-one cylinder 4 is connected with fluid reservoir 5, the slag-drip opening of level-one cylinder 4 and stirring
The entrance of separation chamber 6 is connected, and is sirred and separated in room 6 and is provided with blender, is sirred and separated outlet and the secondary filtration cylinder 7 of room 6
Entrance be connected, the outlet of secondary filtration cylinder 7 is connected with the inlet of the second-order separation cylinder 8, the liquid outlet of the second-order separation cylinder 8
It is connected with fluid reservoir 5, the slag-drip opening of the second-order separation cylinder 8 is connected with the external world.
When Ke Liben series bacillus separation of fermentative broth devices are worked, Ke Liben series bacillus zymotic fluids are put
It sets and is centrifuged in centrifuging room 1, wherein centrifugation room 1 is common centrifugal separation equipment, such as butterfly centrifugal
Machine, it is supernatant and concentrate to centrifuge room 1 by Ke Liben series bacillus separation of fermentative broth, and concentrate is entered by pipeline
In the heavy sugared room of ethyl alcohol 3,95% ethyl alcohol in the heavy sugar room of concentrate and ethyl alcohol 3 comes into full contact with, and the polysaccharide in concentrate is precipitated,
Wherein, in order to improve sedimentation effect, blender can also be set in the heavy sugared room of ethyl alcohol 3, the concentrate after Polysaccharide removing enters one
Carrying out first time filtering in grade cartridge filter 2, (concentrate after Polysaccharide removing can flow into by-pass filtration cylinder 2 by way of overflow
In), the impurity of larger particles is filtered out, filtered concentrate, which enters, carries out UF membrane in level-one cylinder 4, level-one cylinder 4
Concentrate is divided into qualifying liquid and non-qualifying liquid according to preset diameters value, wherein the liquid outlet that qualifying liquid passes through level-one cylinder 4
Into being stored in fluid reservoir 5, non-qualifying liquid is entered by the slag-drip opening of level-one cylinder 4 to be sirred and separated in room 6, stirring point
Non- qualifying liquid is stirred from the blender in room 6, while being passed through and centrifuging the supernatant that room 1 is isolated, avoids stirring
The problem of excessive concentration causes temperature to increase in room, so that the zymotic fluid active constituent mixed in non-qualifying liquid and impurity point
From, and the zymotic fluid active constituent of aggregation is broken up, the non-qualified liquid after being subsequently agitated for flows into secondary filtration cylinder 7, wherein
The filter diameter of secondary filtration cylinder 7 should be not more than the filter diameter of by-pass filtration cylinder 2, and specific size is determined according to actual condition,
Non- qualified liquid after being subsequently agitated for flows into the second-order separation cylinder 8, carries out second of UF membrane, the second-order separation cylinder 8 is according to default
Concentrate is divided into qualifying liquid and non-qualifying liquid by diameter value, wherein qualifying liquid enters liquid storage by the liquid outlet of the second-order separation cylinder 8
It is stored in tank 5, non-qualifying liquid is expelled directly out；Room 6 is sirred and separated using setting i.e. in the utility model, and will be sirred and separated
The entrance of room 6 is connected with supernatant outlet and slag-drip opening, and the outlet for being sirred and separated room 6 is connected with the entrance of secondary filtration cylinder 7
Logical mode so that the waste residue for filtering generation in separator after zymotic fluid progress first separation is stirred separation, avoids giving up
The aggregation of zymotic fluid active constituent leads to that separation is not thorough and the separation of zymotic fluid active constituent is not thorough and leads to separating effect in slag
The problem of difference, meanwhile, it is reused to centrifuging the supernatant generated in room 1, improves the use effect of supernatant
Rate.
Filtering film component includes at least one filter membrane in the utility model.Wherein, it is preferred that filtering film component included
Filter membrane 1, filter membrane 2 10, filter membrane 3 11 and filter membrane 4 12, filter membrane 1, filter membrane 2 10, filter membrane 3 11 and mistake
The filter diameter of filter membrane 4 12 is sequentially reduced, be both provided on the outer wall of level-one cylinder 4 and the second-order separation cylinder 8 slag-drip opening one,
Slag-drip opening two, slag-drip opening three and slag-drip opening four.Further, a diameter of 0.08 μm of the filter hole of filter membrane 1, filter membrane
A diameter of 0.06 μm of 2 10 filter hole, a diameter of 0.04 μm of the filter hole of filter membrane 3 11, the filter hole of filter membrane 4 12 is straight
Diameter is 0.02 μm.
In order to avoid to lead to the problem of temperature in flow process excessively high for concentrate, the heavy sugared room of ethyl alcohol 3 in the utility model
It is provided with cooling chamber 13 between by-pass filtration cylinder 2, spiral is provided between the heavy sugared room 3 of ethyl alcohol and the entrance of by-pass filtration cylinder 2
Coil pipe 14, spiral coil 14 are arranged in cooling chamber 13；I.e. in the utility model cooling chamber is placed on using by spiral coil 14
13 mode makes the fermentation liquid energy in coil pipe be cooled down, and prevents from causing strain dead since temperature is excessively high during conveying
The problem of dying.Wherein, cooling chamber 13 is connected with a circulating water machine 15 in the utility model, the water inlet of circulating water machine 15
It is connected with cooling chamber 13 with water outlet, circulating water machine 15 is existing equipment, which ensure that the cooling temperature in cooling chamber 13
Degree.
In order to improve the cooling-down effect of concentrate, spiral coil 14 is metal thin-wall corrugated pipe coil in the utility model.
In order to improve the separative efficiency of zymotic fluid, spiral coil 14 is connected with a sterile gas supply mechanism in the utility model
It connects, sterile gas supply mechanism includes the desinfection chamber 16 being connected and vacuum pump 17, outlet side and 14 phase of spiral coil of vacuum pump 17
Connection；I.e. in such a way that aseptic gas pushes concentrate, the sport efficiency of concentrate is improved, and then improves point of zymotic fluid
From efficiency.
For the ease of the rear cleaning of use of device, spiral coil 14 is connected with a wiper mechanism in the utility model,
Wiper mechanism includes the water tank 18 being connected and water pump 19, and the water outlet of water pump 19 is connected with spiral coil 14, wherein storage
Water tank 18 is abacterial water tank.
For the temperature value of accurate concentrate, a temperature sensor is provided in the utility model in spiral coil 14.
Specific case is applied in the utility model to be expounded the principles of the present invention and embodiment, it is above
The explanation of embodiment is merely used to help understand the method and its core concept of the utility model；Meanwhile for the one of this field
As technical staff, according to the utility model thought, there will be changes in the specific implementation manner and application range.To sum up
Described, the content of the present specification should not be construed as a limitation of the present invention.
Claims (10)
1. a kind of Ke Liben series bacillus separation of fermentative broth device, which is characterized in that including centrifuging room, the heavy sugar of ethyl alcohol
Room, level-one cylinder, fluid reservoir, is sirred and separated room, secondary filtration cylinder and the second-order separation cylinder, the centrifugation point at by-pass filtration cylinder
From supernatant outlet and concentrated solution outlet is provided on room, the supernatant outlet is connected with the entrance for being sirred and separated room
Logical, the concentrated solution outlet is connected with the entrance of the heavy sugared room of the ethyl alcohol, the outlet of the heavy sugared room of the ethyl alcohol and the level-one
The entrance of cartridge filter is connected, and is both provided with filtering film component in the level-one cylinder and the second-order separation cylinder, and described one
It is both provided with inlet, slag-drip opening and liquid outlet, the level-one cylinder on the outer wall of grade cylinder and the second-order separation cylinder
Inlet be connected with the outlet of the by-pass filtration cylinder, the liquid outlet of the level-one cylinder is connected with the fluid reservoir
Logical, the slag-drip opening of the level-one cylinder is connected with the entrance for being sirred and separated room, and the sirring and separating interior is provided with
Blender, the outlet for being sirred and separated room are connected with the entrance of the secondary filtration cylinder, the outlet of the secondary filtration cylinder
It is connected with the inlet of the second-order separation cylinder, the liquid outlet of the second-order separation cylinder is connected with the fluid reservoir, described
The slag-drip opening of the second-order separation cylinder is connected with the external world.
2. Ke Liben series bacillus separation of fermentative broth device according to claim 1, which is characterized in that the filter membrane
Component includes at least one filter membrane.
3. Ke Liben series bacillus separation of fermentative broth device according to claim 2, which is characterized in that the filter membrane
Component includes filter membrane one, filter membrane two, filter membrane three and filter membrane four, the filter membrane one, filter membrane two, three and of filter membrane
The filter diameter of filter membrane four is sequentially reduced, and deslagging is both provided on the outer wall of the level-one cylinder and the second-order separation cylinder
Mouth one, slag-drip opening two, slag-drip opening three and slag-drip opening four.
4. Ke Liben series bacillus separation of fermentative broth device according to claim 3, which is characterized in that the filtering
A diameter of 0.08 μm of the filter hole of film one, a diameter of 0.06 μm of the filter hole of the filter membrane two, the filtering of the filter membrane three
Bore dia is 0.04 μm, a diameter of 0.02 μm of the filter hole of the filter membrane four.
5. Ke Liben series bacillus separation of fermentative broth device according to claim 1, which is characterized in that the ethyl alcohol is heavy
Cooling chamber is provided between sugared room and the by-pass filtration cylinder, between the heavy sugared room of the ethyl alcohol and the entrance of the by-pass filtration cylinder
It is provided with spiral coil, the spiral coil is arranged in the cooling chamber.
6. Ke Liben series bacillus separation of fermentative broth device according to claim 5, which is characterized in that the cooling chamber
It is connected with a circulating water machine, the inlet and outlet of the circulating water machine are connected with the cooling chamber.
7. Ke Liben series bacillus separation of fermentative broth device according to claim 5, which is characterized in that the helical disk
Pipe is metal thin-wall corrugated pipe coil.
8. Ke Liben series bacillus separation of fermentative broth device according to claim 5, which is characterized in that the helical disk
The sterile gas supply mechanisms of Guan Yuyi are connected, and the sterile gas supply mechanism includes the desinfection chamber and vacuum pump being connected, the vacuum
The outlet side of pump is connected with the spiral coil.
9. Ke Liben series bacillus separation of fermentative broth device according to claim 5, which is characterized in that the helical disk
Guan Yuyi wiper mechanisms are connected, and the wiper mechanism includes the water tank and water pump being connected, the water outlet of the water pump with
The spiral coil is connected.
10. Ke Liben series bacillus separation of fermentative broth device according to claim 5, which is characterized in that the spiral
A temperature sensor is provided in coil pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820337741.2U CN208055329U (en) | 2018-03-12 | 2018-03-12 | A kind of Ke Liben series bacillus separation of fermentative broth device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820337741.2U CN208055329U (en) | 2018-03-12 | 2018-03-12 | A kind of Ke Liben series bacillus separation of fermentative broth device |
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|---|---|
| CN208055329U true CN208055329U (en) | 2018-11-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820337741.2U Expired - Fee Related CN208055329U (en) | 2018-03-12 | 2018-03-12 | A kind of Ke Liben series bacillus separation of fermentative broth device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115651822A (en) * | 2022-09-08 | 2023-01-31 | 马鞍山同杰良生物材料有限公司 | System and method for continuously separating thalli in fermentation production |
-
2018
- 2018-03-12 CN CN201820337741.2U patent/CN208055329U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115651822A (en) * | 2022-09-08 | 2023-01-31 | 马鞍山同杰良生物材料有限公司 | System and method for continuously separating thalli in fermentation production |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181106 Termination date: 20210312 |