CN208043644U - Portable milk protein content device for fast detecting - Google Patents

Portable milk protein content device for fast detecting Download PDF

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Publication number
CN208043644U
CN208043644U CN201820194367.5U CN201820194367U CN208043644U CN 208043644 U CN208043644 U CN 208043644U CN 201820194367 U CN201820194367 U CN 201820194367U CN 208043644 U CN208043644 U CN 208043644U
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China
Prior art keywords
sensor
light source
protein content
cuvette
arithmetic
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CN201820194367.5U
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Chinese (zh)
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郭文川
杨彪
王凯
王彦增
朱新华
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Northwest A&F University
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Northwest A&F University
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Abstract

The utility model discloses a kind of portable milk protein content device for fast detecting, belong to composition detection technical field.The technical issues of solution, is to provide a kind of portable milk protein content device for fast detecting at low cost, detection speed is fast.Composed structure includes display, button, arithmetic and control unit, power management module, switch, accumulator, UV sensor, cuvette, cuvette holder, light source, shell.Light source and UV sensor are mounted on cuvette holder, the light that light source is sent out passes through the milk sample in cuvette and cuvette to be received by UV sensor, arithmetic and control unit receives the control signal from button and the absorbance signal from UV sensor, calculates the protein content of milk and the results are shown on display.The utility model can quickly detect milk protein content, and decision-making foundation is provided for consumer.

Description

Portable milk protein content device for fast detecting
Technical field
The utility model is related to composition detection technical field, more particularly to a kind of portable milk protein content is quickly examined Survey device.
Background technology
Protein is one of the Major Nutrient substance in milk, and the protein content in milk can reflect its quality. 19645-2010 regulation Protein Content in Milk of standard GB/T should be greater than being equal to 2.9g/100ml, national standard The milk protein detection method of content of three kinds of standards is described in the measurement of 2016 Protein in Food of GB5009.5-, respectively It is Kjeldahl's method, spectrophotometry and combustion method.These three methods belong to chemical method, time-consuming there are complex for operation step Arduously, the defects of with high costs.
China Patent Publication No. CN201569625U, publication date on 09 01st, 2010, invention and created name are " a hatching egg White matter detecting instrument ", that application discloses " a kind of protein detection instrument, including light source, colorimetric pool and detector, features It is:Microprocessor is controlled by signal and is connect respectively with light source, detector with amplification module;The light transmission that light source is sent out crosses ratio Color pond is detected by detector, and detector sends the signal detected to microprocessor (5) through signal control with amplification module, believes Number control with amplification module control light source, microprocessor respectively with display module, printer and by key connection;Colorimetric bottle be located at than In color pond, colorimetric bottle is prolate cube." be somebody's turn to do " protein detection instrument " be characterized in that " photoelectric sensor use TSL series collection At photoelectric sensor ".TSL series photoelectric sensors be silicon based sensor, operating wavelength range is visible light, thus exist easily by The deficiency that ambient light influences.Protein is in visible light wave range without obvious absorption peaks, it is therefore desirable to use the acid mentioned in the patent Property -12 equal coloring agents of orange could realize that protein detection, operating procedure are more.In addition it how is not provided in the patent by light The output signal of electric transducer is converted to the specific method of protein content, therefore realisation relatively low.
China Patent Publication No. CN102590100A, publication date on 07 18th, 2012, invention and created name be " eosin and Application of its derivative in protein detection ", that application discloses " eosin and its derivative answering in protein detection With " " it includes step:1) the protein example gel after SDS-PAGE electrophoresis is placed in fixer and fixes 10~ 60min abandons fixer, is then rinsed 1~3 time with deionized water, 1~10min every time;2) in methanol solution balance 5~ The methanol aqueous solution that 20min, wherein methanol solution are by volume 30~70%;3) 1~30min of dyeing liquor is added, then puts 1~10min is rinsed in deionized water, and wherein dyeing liquor is eosin containing w/v 0.1~2% or derivatives thereof and presses The methanol of volume ratio 30~70%, 0.5~1.5% acetic acid aqueous solution.Then 1~10min of rinsing in deionized water is put;4) Detection." this method have problems in that operating procedure complexity, need special and expensive instrument.
In conclusion there are complicated for operation, testing cost height, the problems such as being easily disturbed for the prior art.
Invention content
Stablize the technical problem to be solved by the present invention is to provide a kind of of low cost, easy to operate, test result Portable milk protein content device for fast detecting.
The utility model is achieved through the following technical solutions:
Portable milk protein content device for fast detecting, composed structure include display, button, arithmetic and control unit, Power management module, switch, accumulator, UV sensor, cuvette, cuvette holder, light source, shell;The light source and There are two UV sensor is each, two light sources are installed in the side of cuvette holder, and UV sensor is mounted on colorimetric On ware holder with the other side of light source face;The arithmetic and control unit, button, accumulator, display, power management module, ratio Color ware holder is respectively mounted on the shell;UV sensor, button, display, power management module with arithmetic and control unit phase Even;The accumulator is connected with power management module;The light source is controlled by switch, is connected with power management module.
Light source can send out the ultraviolet light that wavelength is 280nm and 260nm respectively;UV sensor is the special biography of ultraviolet light Sensor, to visible light without response;Arithmetic and control unit has analog-digital conversion function;Power management module can give light source, ultraviolet light Sensor, arithmetic and control unit, keyboard, display provide suitable work pressure and operating current, and can be with management of battery Charge and discharge.
Detection method includes the following steps and carries out in the following order for the utility model:
A. preparation:By lower switch, portable milk protein content device for fast detecting is opened, is preheated 15 minutes; Milk sample is taken, adds deionized water to dilute n times, obtains prepare liquid, prepare liquid is added in cuvette.
B. absorbance is measured:The 280nm wavelength light holes cuvette for filling prepare liquid being put into cuvette holder;It presses First button in lower button, the data A that arithmetic and control unit detects UV sensor280It reads and preserves;It will fill The cuvette of prepare liquid is put into the 260nm wavelength light holes in cuvette holder, presses second button in lower button, operation control Device processed(3)The data A that UV sensor is detected260It reads and preserves.
C. it calculates milk protein content and shows result of calculation:Arithmetic and control unit is obtaining data A280 And A260Later Start to calculate the protein of milk to be measured, and result of calculation is shown over the display.Calculate milk protein algorithm be:
K in formula1And k2For constant term, P is protein content.
The measuring principle of the utility model, which is the protein in milk, has obviously wavelength for the ultraviolet light near 280nm Absorption, nucleic acid to wavelength be 260nm ultraviolet light also have apparent absorption.Both material absorbings in milk Peak is closer from obtaining, and therefore, in order to detect the protein content in milk, and reduces nucleic acid to accuracy of detection influence, needs to distinguish Detect absorbance of the milk at 280nm and 260nm.After measuring the two absorbances, pass through calculating, so that it may to obtain milk Middle protein content.Since the protein content in milk is relatively high, need milk diluting several times before detection, So that detection luminous energy is smoothly through detection sample.
Compared with prior art, the utility model has the advantage of:
A, easy to operate, detection speed is fast, at low cost.The utility model only needs milk to be measured diluting several times, i.e., It can be put into detector and detect, not need staining procedure required when visible light wave range detection protein, also do not need other changes The large-scale instrument that method detection needs.
B, the utility model considers influence of the nucleic acid to protein content accuracy of detection, and provides and reduce the influence Specific method, improve accuracy of detection.
Reference numeral
1, display;2, button 3, arithmetic and control unit;4, power management module;5 switches;6 accumulators;7 ultraviolet lights sense Device;8, cuvette;9, cuvette holder;10, light source;11, shell.
Description of the drawings
Fig. 1 is the structure chart of the utility model.
Specific implementation mode
With reference to a preferred embodiments and drawings, the utility model is described in further detail:
As shown in Figure 1, portable milk protein content device for fast detecting, composed structure include display 1, button 2, Arithmetic and control unit 3, power management module 4, switch 5, accumulator 6, UV sensor 7, cuvette 8, cuvette holder 9, light Source 1, shell 1;There are two the light source 10 and UV sensor 7 are each, two light sources 10 are installed in the one of cuvette holder 9 Side, UV sensor 7 are mounted on the other side with 10 face of light source on cuvette holder 9;The arithmetic and control unit 3, button 2, accumulator 6, display 1, power management module 4, cuvette holder 9 are installed on shell 11;UV sensor 7 is pressed Button 2, display 1, power management module 4 are connected with arithmetic and control unit 3;The accumulator 6 is connected with power management module 4; The light source 10 is controlled by switch 5, is connected with power management module 4.
It is the ultraviolet LED of 280nm and 260nm that light source, which selects emission wavelength,.
UV sensor selects UVM-30A types.
Arithmetic and control unit selects the STC12C5A60S2 core boards with analog-digital conversion function.
Keyboard is made of a switch and two buttons, and switch is connected in the general supply of instrument, and button is connected on operation control On the I/O mouths of device.
Accumulator selects two 18650 type lithium batteries of section.
Display selects the OLED12864 based on SSD1306 chips.
Power management module selects DD06CVSA type charging and discharging lithium battery modules.
Cuvette selects quartz colorimetric utensil.
Portable milk protein method for quickly detecting contents includes the following steps and carries out in the following order:
A. preparation:By lower switch 5, portable milk protein content device for fast detecting is opened, is preheated 15 minutes; Milk sample is taken, adds deionized water to dilute 100 times, obtains prepare liquid, prepare liquid is added in cuvette 8.
B. absorbance is measured:The 280nm wavelength light holes cuvette 8 for filling prepare liquid being put into cuvette holder 9; Press first button in lower button 2, the data A that arithmetic and control unit 3 detects UV sensor 7280It reads and preserves; The cuvette 8 for filling prepare liquid is put into the 260nm wavelength light holes in cuvette holder 9, is pressed by second in lower button 2 Button, the data A that arithmetic and control unit 3 detects UV sensor 7260It reads and preserves.
C. it calculates milk protein content and shows result of calculation:Arithmetic and control unit 3 is obtaining data A280And A260Later Start to calculate the protein of milk to be measured, and includes on display 1 by result of calculation.Calculate milk protein algorithm be:
K in formula1And k2For constant term, P is protein content.
Above example is only to the utility model for example, not constituting to the scope of protection of the utility model Limitation, every design same or analogous with the utility model both is within the protection scope of the present invention.

Claims (3)

1. portable milk protein content device for fast detecting, it is characterised in that composed structure includes display(1), button (2), arithmetic and control unit(3), power management module(4), switch(5), accumulator(6), UV sensor(7), cuvette (8), cuvette holder(9), light source(10), shell(11);The light source(10)And UV sensor(7)There are two each, two A light source(10)It is installed in cuvette holder(9)Side, UV sensor(7)Mounted on cuvette holder(9)It is upper with Light source(10)The other side of face;The arithmetic and control unit(3), button(2), accumulator(6), display(1), power management mould Block(4), cuvette holder(9)It is installed in shell(11)On;UV sensor(7), button(2), display(1), power supply Management module(4)And arithmetic and control unit(3)It is connected;The accumulator(6)With power management module(4)It is connected;The light source (10)It is switched(5)Control, with power management module(4)It is connected.
2. portable milk protein content device for fast detecting according to claim 1, it is characterised in that:The light source (10)The ultraviolet light that wavelength is 280nm and 260nm can be sent out respectively;The UV sensor(7)For the special sensing of ultraviolet light Device, to visible light without response;The arithmetic and control unit(3)Has analog-digital conversion function;The power management module(4)It can give Light source(10), UV sensor(7), arithmetic and control unit(3), button(2), display(1)Suitable work pressure and work are provided Make electric current, and can be with management of battery(6)Charge and discharge.
3. portable milk protein content device for fast detecting according to claim 2, it is characterised in that:The colorimetric Ware(8)For quartz material.
CN201820194367.5U 2018-02-05 2018-02-05 Portable milk protein content device for fast detecting Active CN208043644U (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108333134A (en) * 2018-02-05 2018-07-27 西北农林科技大学 Portable milk protein method for quickly detecting contents and device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108333134A (en) * 2018-02-05 2018-07-27 西北农林科技大学 Portable milk protein method for quickly detecting contents and device

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