CN207958391U - A kind of excretion body extraction element - Google Patents
A kind of excretion body extraction element Download PDFInfo
- Publication number
- CN207958391U CN207958391U CN201721807225.3U CN201721807225U CN207958391U CN 207958391 U CN207958391 U CN 207958391U CN 201721807225 U CN201721807225 U CN 201721807225U CN 207958391 U CN207958391 U CN 207958391U
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- wall
- box
- excretion body
- babinet
- motor
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Abstract
The utility model belongs to field of biochemistry equipment, discloses a kind of excretion body extraction element, is provided with babinet, and motor is secured by bolts in the bottom of box, and centrifugal basket is secured by bolts in the shaft of motor；Ultrasonic transmitter is secured by bolts on wall box, and settling box is secured by bolts in the bottom of box；Display screen is bolted on the outer wall of babinet front, and the control panel is bolted on the positive outer wall of babinet；The temperature sensor is pasted onto by seccotine on the inner wall of box house, and the motor, display screen, temperature sensor are connected by data line with control panel.Easy to operate, time-consuming short, the simplified operating instruction of the excretion body extraction element, it is cost-effective.Rotating speed when control panel is used to control motor speed and then controls centrifugal basket centrifugation, emits ultrasonic intensity for controlling ultrasonic transmitter, makes it possible routine clinicalization of body fluid biopsy.
Description
Technical field
The utility model belongs to field of biochemistry equipment more particularly to a kind of excretion body extraction element.
Background technology
Currently,
In recent years, the research of excretion body and understanding are deepened with people, excretion physical examination is surveyed as a kind of novel liquid
Biopsy hot spot technology has been widely used in non-invasive diagnosis, treatment and the monitoring of tumour and disease by many clinical research mechanisms；
How efficiently to extract excretion body is the key that realize this emerging liquid Biopsy routine clinicalization application.Excretion body at present
The standard of extraction is still supercentrifugation；Method is sufficiently complex cumbersome, and time-consuming, for extracting the excretion body in urine,
It at least needs 200 milliliters of amount of urine every time, and could be obtained enough by ultracentrifugation (120000g/ minute) 20 hours or more
Enough excretion scale of constructions, while the training of the equipment requirement and operation sequence to ultracentrifuge substantially increases extraction cost,
It cannot achieve the clinical application that routinizes.Due to the domestic shortage in relation to excretion body extracts reagent, research of the China to excretion body
The cumbersome ultracentrifugation of process and import extracts kit are also relied on substantially, therefore also seriously hinders China in excretion body
Development on studies and clinical application.
In conclusion problem of the existing technology is：Domestic related excretion body extracts reagent lacks, and extracts kit is deficient
Weary, related excretion body extraction element lacks, and cannot be satisfied the needs of scientific research person.
Utility model content
In view of the problems of the existing technology, the utility model provides a kind of excretion body extraction element.
The utility model is realized in this way the excretion body extraction element is provided with babinet, motor is secured by bolts in
The bottom of box, centrifugal basket are secured by bolts in the shaft of motor；Ultrasonic transmitter is secured by bolts in case-side
On wall, settling box is secured by bolts in the bottom of box；
Display screen is bolted on the outer wall of babinet front, and it is positive that the control panel is bolted on babinet
On outer wall；The temperature sensor is pasted onto by seccotine on the inner wall of box house, and the motor, display screen, temperature pass
Sensor is connected by data line with control panel.
Further, excretion body extractant test tube is connected on the wall box；PBS test tubes are connected on wall box.
Further, the motor connects externally fed power supply by frequency converter.
Further, heat sink is embedded on the outer wall of the babinet.
The utility model has the advantage that and actively has the technical effect that：The excretion body extraction element it is easy to operate, take
It is short, simplify operating instruction, it is cost-effective.Rotating speed when control panel is used to control motor speed and then controls centrifugal basket centrifugation,
Emit ultrasonic intensity for controlling ultrasonic transmitter, makes it possible routine clinicalization of body fluid biopsy.
Description of the drawings
Fig. 1 is the excretion body extraction element structural schematic diagram that the utility model embodiment provides；
In figure：1, babinet；2, centrifugal basket；3, shaft；4, motor；5, ultrasonic transmitter；6, settling box；7, excretion body
Extractant test tube；8, PBS test tubes；9, display screen；10, control panel；11, temperature sensor；12, heat sink.
Specific implementation mode
For that can further appreciate that the utility model content, feature and effect of the utility model, the following examples are hereby given, and
It is described in detail with the accompanying drawings as follows.
The structure of the utility model is explained in detail below in conjunction with the accompanying drawings.
As shown in Figure 1, the excretion body extraction element is provided with babinet 1, motor 4 is secured by bolts in 1 bottom of babinet, from
Heart rotating cylinder 2 is secured by bolts in the shaft 3 of motor 4；Ultrasonic transmitter 5 is secured by bolts on 1 side wall of babinet, is sunk
Shallow lake bucket 6 is secured by bolts in 1 bottom of babinet；
Display screen 9 is bolted on 1 front outer wall of babinet, and the control panel 10 is bolted on babinet 1
On positive outer wall；The temperature sensor 11 is pasted onto by seccotine on the inner wall inside babinet 1, the motor 4, display
Screen 9, temperature sensor 11 are connected by data line with control panel 10.
As the preferred embodiment of the utility model, excretion body extractant test tube 7 is connected on 1 side wall of the babinet；PBS
Test tube 8 is connected on 1 side wall of babinet.
As the preferred embodiment of the utility model, the motor 4 connects externally fed power supply by frequency converter.
As the preferred embodiment of the utility model, heat sink 12 is embedded on the outer wall of the babinet 1.
When the utility model is used, including the following steps：By humoral sample by centrifugal basket 2, starting motor 4,
Centrifugal basket 2 is centrifuged by the rotation of shaft 3, is opened simultaneously ultrasonic transmitter 5 and is carried out ultrasonic Separation, 5-15 points of centrifugation
Clock takes its supernatant；Extracts reagent in excretion body extractant test tube 7 is added in above-mentioned steps in obtained supernatant,
After reaction 2 hours, by centrifugal basket 2, starting motor 4, centrifugal basket 2 is centrifuged by the rotation of shaft 3, centrifuges 5-
10 minutes, the volume ratio of the extracts reagent and supernatant was 1：2-20；The excretion body sediment that above-mentioned steps obtain is taken, then
It carries out the suspension of excretion body sediment in merging PBS test tubes 8 to dissolve again, dissolved matter, which is placed in precipitation in settling box 6, obtains excretion body.
Excretion body extractant is contained in excretion body extractant test tube 7, the extracts reagent includes as following formula：0.01-
The dithiothreitol (DTT) of 0.5mM, the potassium chloride of 10-50mM, the potassium citrate of 0.05-0.5mM, the sodium formate of 0.1-1M, 0.15uM-
The guanidine thiocyanate of the seralbumin of 3uM, the glycine of 0.01-1mM, 0.4mM-2.5mM.
Display screen 8 is connected with motor 4, ultrasonic transmitter 5, temperature sensor 11 respectively by conducting wire.For showing
Rotating speed when centrifugal basket 2 centrifuges, the intensity for showing 5 emitted ultrasonic wave of ultrasonic transmitter are passed for displays temperature
The temperature in babinet 1 that sensor 11 is incuded.
Control panel 10 is connected with motor 4, ultrasonic transmitter 5, heat sink 12 respectively by conducting wire.For controlling electricity
Rotating speed when 4 rotating speed of machine and then the control centrifugation of centrifugal basket 2, emits ultrasonic intensity for controlling ultrasonic transmitter 5, is used for
Control the switch of heat sink 12.11 temperature sensor of temperature sensor is shown on display screen 8, when the temperature is excessively high, opens drop
Warm device 12.
The above is only the preferred embodiment to the utility model, is not made in any form to the utility model
Limitation, it is every according to the technical essence of the utility model it is made to the above embodiment it is any it is simple modification, equivalent variations with
Modification, is all within the scope of the technical scheme of the utility model.
Claims (4)
1. a kind of excretion body extraction element, which is characterized in that the excretion body extraction element is provided with babinet, and motor passes through bolt
It is fixed on the bottom of box, centrifugal basket is secured by bolts in the shaft of motor；Ultrasonic transmitter is secured by bolts in
On wall box, settling box is secured by bolts in the bottom of box；
Display screen is bolted on the outer wall of babinet front, and control panel is bolted on the positive outer wall of babinet；
Temperature sensor is pasted onto by seccotine on the inner wall of box house, and the motor, display screen, temperature sensor pass through number
It is connected with control panel according to line.
2. excretion body extraction element as described in claim 1, which is characterized in that excretion body extractant test tube is connected in the case
On body sidewall；PBS test tubes are connected on wall box.
3. excretion body extraction element as described in claim 1, which is characterized in that the motor connects external supply by frequency converter
Power supply.
4. excretion body extraction element as described in claim 1, which is characterized in that embedded with cooling dress on the outer wall of the babinet
It sets.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721807225.3U CN207958391U (en) | 2017-12-21 | 2017-12-21 | A kind of excretion body extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721807225.3U CN207958391U (en) | 2017-12-21 | 2017-12-21 | A kind of excretion body extraction element |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207958391U true CN207958391U (en) | 2018-10-12 |
Family
ID=63729441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721807225.3U Expired - Fee Related CN207958391U (en) | 2017-12-21 | 2017-12-21 | A kind of excretion body extraction element |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207958391U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112275455A (en) * | 2020-10-12 | 2021-01-29 | 广州爱索达生物医药技术有限公司 | Exosome particle screening device based on ultracentrifugation technology |
-
2017
- 2017-12-21 CN CN201721807225.3U patent/CN207958391U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112275455A (en) * | 2020-10-12 | 2021-01-29 | 广州爱索达生物医药技术有限公司 | Exosome particle screening device based on ultracentrifugation technology |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181012 Termination date: 20191221 |
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| CF01 | Termination of patent right due to non-payment of annual fee |