CN208752085U - One specific cells in body captures box - Google Patents

Abstract

The utility model discloses the specific cells in body to capture box, includes capture box, and blood entry port and blood outlet can be equipped with the biomarker in conjunction with specific cells, the capture box by being equipped in the capture box.The utility model structure is simple, can carry out the early screening of tumour, the drug susceptibility of tumour, tumor load, antibiotics sensitivity of bacterium etc..For the utility model without blood drawing, the cell of capture is more, at low cost, easy to operate, and efficiently, nontoxic.

Description

One specific cells in body captures box

Technical field:

The utility model relates to the specific cells in body to capture box.

Background technique:

Cancer is still the number one killer of the mankind so far, and the newly-increased cases of cancer 14,100,000 in the whole world in 2015,8,200,000 people are dead In cancer.Research shows that most of tumor patient dies of metastatic tumor, rather than primary tumor；Metastases are to lead to cancer patient The main reason for dead.By current medical level, to the infantile tumour patient not yet shifted, there are about 80%-90% or more to control More；But for the tumor patient in advanced stage, survival rate is only 8-10% within 5 years.Therefore, for the early detection of metastases and swollen Curative effect monitoring in tumor therapeutic process has decisive significance to the treatment of tumour.Currently, in diagnosing tumor and treatment, doctor The lesions position of tumour is usually determined using imaging means (such as PET-CT), and uses invasive solid tumor biopsy It looks into qualitative to tumour and parting by stages is carried out to tumour.Current this detection mode has the disadvantages that 1, can not achieve The early detection of real meaning；2, expensive；3, invasive inspection, it is larger to patient's injury, it cannot frequently use；4, cannot Realize real-time detection.

Circulating tumor cell refers to the tumour cell that blood circulation system is detached and entered from primary tumor or transfer stove, These cells to form the potential of new neoplasm metastasis with adhering at other positions of body.Metastases are often because follow Caused by ring tumour cell, the discovery of circulating tumor cell can be used as one of indication of metastases.These heterogeneities are followed Ring tumour cell has very strong anti-apoptotic ability, transfer incidence, chemotherapeutic efficacy, recurrence and existence with tumor patient Time is closely related, thus become metastases, recurrence and more after important indicator.But for leucocyte, circulation Tumour cell is considerably less, if we can extract circulating tumor cell, cooperates currently popular slender extracellular molecule sequencing Technology, we can be in position, parting and the mutational site for determining that tumour in early days, and it is suitable just to formulate in early stage in this way Therapeutic scheme strangles cancer in rudiment；Cooperation immunostaining technique and cell count can analyze tumor load and tumour The effect for the treatment of；Cooperation drug study can be obtained by the Drug sensitivity data of tumour, according to the adjustable neoplastic disease of data The therapeutic scheme of people.

The method that most commercialization CTC detectors are all made of vitro detection both at home and abroad at present, the technology of CTC is with the U.S. The CellSearch FCM analysis instrument of johnsen diagnostic companies and the IsoFlux of Fluxion company are representative, this two set The standby certification for all obtaining U.S. FDA.There are many detection method of extracorporal circulatory system tumour cell, can be divided into yin according to the method for enrichment Property enrichment method and positive enrichment method.Principle is dependent on external blood drawing and cell separation technology, although circulating tumor cell is examined Survey technology has application prospect very much, however technically there are still defects.Such as operate complicated, recall rate is low and by blood drawing amount Limit (< 15ml) etc..It is limited to total blood sampling volume, the sensitivity of the detection of these external circulating tumor cell monitors is normal Requirement cannot often be reached.

Utility model content:

The utility model aim is exactly in order to make up the defect of prior art, to provide the specific cells in body and capture Box.The utility model can separate many things from blood, but main effect is that circulating tumor cell is separated from blood. The purpose of separation circulating tumor cell is to carry out the liquid biopsy of early stage.

The utility model is achieved through the following technical solutions:

One specific cells in body captures box, it is characterised in that: includes capture box, equipped with can be in the capture box The biomarker that specific cells combine, the capture box is equipped with blood entry port and blood exports.

Described one captures box in the specific cells of body, it is characterised in that: the blood entry port and blood outlet are upper equal It is connected with perfusion tube, perfusion tube outer end is equipped with syringe needle, is equipped with adjuster on perfusion tube.

Described one captures box in the specific cells of body, it is characterised in that: is equipped with blood transfusion apparatus on any perfusion tube.

Described one body specific cells capture box, it is characterised in that: the specific cells be circulating tumor cell, Immunocyte, bacterium or the cell that can be separated from blood.

Described one captures box in the specific cells of body, it is characterised in that: the biomarker is antibody or nucleic acid Aptamers.

Described one captures box in the specific cells of body, it is characterised in that: biomarker passes through covalent coupling, molecule Between interaction force or power is fixed in capture box the effects of hydrogen bond.

Described one captures box in the specific cells of body, it is characterised in that: is equipped with gel or biomembrane, institute in capture box Biomarker is stated to be fixed on gel or biomembrane.

Described one captures box in the specific cells of body, it is characterised in that: magnetic bead, the biology mark are equipped in capture box Note object is fixed on magnetic bead.

The perfusion tube is corollary apparatus.

There are many acquisition modes of the utility model, including artery-vein connection capture, vein-vein connection capture.It is dynamic Arteries and veins-vein connection acquisition mode is to be connected to artery with vein by the perfusion tube at both ends, make blood under the promotion of blood pressure into Enter to capture box, thus in conjunction with biomarker.Vein-vein connection acquisition mode be by the perfusion tube at both ends by vein and Vein connection, is pumped into capture box for blood pressure under the action of pump.

Capture box can have various shape, specification, color and material.

When the utility model is used, blood vessel is inserted by the needle on perfusion tube respectively, can be artery and vein, it can also be with It is vein and vein, blood is allowed to flow through the capture box among two needles, having in capture box can be with circulating tumor cell specificity In conjunction with biomarker.Blood is allowed to flow in capture box, after the regular hour, all blood of human body can all pass through Box is captured, more circulating tumor cells can be captured in this way, and does not need to extract blood, is not limited by amount for taking blood.

Requirement of the capture box for specification, color, material etc. in the utility model is few, nontoxic.

Capture box is a kind of hollow structure, and main effect is that the biology that storage can be specifically bound with specific cells is marked Remember object.As corollary apparatus, box it is of less demanding, as long as inner space is slightly a little bigger, nontoxic.It can basis The fixed method of cell category, the biomarker of capture, specification, shape, the material of different change boxes of experiment purpose etc..

The effect of biomarker in capture box is needed to secure it on magnetic bead or box in conjunction with specific cells In the inside of son or box on other materials, so that specific cells are enriched with.Biomarker can be various types of Antibody and aptamer.The specificity of biomarker need it is high a bit, but since blood volume is big, the cell of capture compared with More, specificity can also be without too high, this is also an advantage of our utility model.Biomarker can be according to capture The type of cell, the difference of the purpose of experiment and replace the fixing means of different biomarker and biomarker.

The utility model can realize the highly selective and high specific specific cell of capture in body.Divide after the completion of capture From corollary apparatus and capture box, after capturing box with normal saline flushing, being eluted the cell of capture using eluent can To carry out analyzing for next step.

The advantages of the utility model are as follows:

The utility model structure is simple, can carry out the early screening of tumour, the drug susceptibility of tumour, tumor load, Antibiotics sensitivity of bacterium etc..The utility model is without blood drawing, and the cell of capture is more, at low cost, easy to operate, Er Qiegao Effect, it is nontoxic.

Detailed description of the invention:

Fig. 1 is the structural schematic diagram of the utility model.

Specific embodiment:

Referring to attached drawing.

One specific cells capture box in body includes capture box 1, and being equipped in the capture box can be with specific cells knot The biomarker 2 of conjunction, the capture box 1 is equipped with blood entry port and blood exports.

It is respectively connected with perfusion tube 3 outside the blood entry port and blood outlet, 3 outer end of perfusion tube is equipped with syringe needle 4, perfusion tube Adjuster 5 is installed on 3.

Blood transfusion apparatus 6 is installed on any perfusion tube 3.

The specific cells are circulating tumor cell, immunocyte, bacterium or the cell that can be separated from blood.

The biomarker is antibody or aptamer.

Biomarker is fixed in capture box by power the effects of covalent coupling, intermolecular interaction or hydrogen bond.

It captures and is equipped with gel or biomembrane in box 1, the biomarker is fixed on gel or biomembrane.

It captures and is equipped with magnetic bead in box 1, the biomarker is fixed on magnetic bead.

The operating process of one specific cells capture box in body is as follows:

(1) it first has to rinse one time inside the utility model using physiological saline, directly by transfusion needle insertion bottle Infusion bottle allows physiological saline to flow down naturally.The effect of this step is to allow liquid full of capture box and transfusion needle, prevents liquid gas Body enters human body, but also can rinse capture box.

(2) blood vessel will be penetrated respectively with the needle in the corollary apparatus that is connected of capture box both ends by label, if it is artery with Vein just allows blood to flow in capture box.If it is vein and vein, it is necessary to start matched peristaltic pump to provide Power.To the regular hour, so that it may remove corollary apparatus, separation corollary apparatus and capture box, be caught with normal saline flushing Obtain box.

(3) cell of capture is washed using cell elution reagent, then can be carried out the processing analysis of next step , can also be enriched with again primary.Experimental purpose can choose laboratory cultures, antibiotics sensitivity, sequencing, unicellular The experiment such as sequencing, immunostaining, cell count and drug susceptibility.There are also transparent box bodys for our capture box, facilitate experiment The case where the case where member directly observes the cell of capture through box body and dyeing.

Claims (8)

1. the specific cells in body capture box, it is characterised in that: include capture box, being equipped in the capture box can be with spy Determine the biomarker of cell combination, the capture box is equipped with blood entry port and blood exports.

2. the one according to claim 1 specific cells in body capture box, it is characterised in that: the blood entry port and blood Liquid is respectively connected with perfusion tube in outlet, and perfusion tube outer end is equipped with syringe needle, is equipped with adjuster on perfusion tube.

3. the one according to claim 2 specific cells in body capture box, it is characterised in that: installed on any perfusion tube There is blood transfusion apparatus.

4. the one according to claim 1 to 3 specific cells in body capture box, it is characterised in that: described specific Cell is circulating tumor cell, immunocyte, bacterium or the cell that can be separated from blood.

5. the one according to claim 4 specific cells in body capture box, it is characterised in that: the biomarker is Antibody or aptamer.

6. the one according to claim 5 specific cells in body capture box, it is characterised in that: biomarker passes through altogether Valence coupling, intermolecular interaction or power is fixed in capture box the effects of hydrogen bond.

7. the one according to claim 5 specific cells in body capture box, it is characterised in that: be equipped with gel in capture box Or biomembrane, the biomarker are fixed on gel or biomembrane.

8. the one according to claim 5 specific cells in body capture box, it is characterised in that: be equipped with magnetic in capture box Pearl, the biomarker are fixed on magnetic bead.