CN108761080A - One specific cells in body captures box - Google Patents
One specific cells in body captures box Download PDFInfo
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- CN108761080A CN108761080A CN201810934966.0A CN201810934966A CN108761080A CN 108761080 A CN108761080 A CN 108761080A CN 201810934966 A CN201810934966 A CN 201810934966A CN 108761080 A CN108761080 A CN 108761080A
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- 239000008280 blood Substances 0.000 claims abstract description 34
- 210000004369 blood Anatomy 0.000 claims abstract description 34
- 239000000090 biomarker Substances 0.000 claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 210000004027 cell Anatomy 0.000 claims description 46
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 9
- 239000011324 bead Substances 0.000 claims description 6
- 108091023037 Aptamer Proteins 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 239000001257 hydrogen Substances 0.000 claims description 3
- 229910052739 hydrogen Inorganic materials 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000009878 intermolecular interaction Effects 0.000 claims description 2
- 206010028980 Neoplasm Diseases 0.000 abstract description 29
- 239000003814 drug Substances 0.000 abstract description 5
- 229940079593 drug Drugs 0.000 abstract description 5
- 231100000252 nontoxic Toxicity 0.000 abstract description 4
- 230000003000 nontoxic effect Effects 0.000 abstract description 4
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 229940088710 antibiotic agent Drugs 0.000 abstract description 2
- 238000012216 screening Methods 0.000 abstract description 2
- 210000003462 vein Anatomy 0.000 description 10
- 238000001514 detection method Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 206010027476 Metastases Diseases 0.000 description 6
- 201000011510 cancer Diseases 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 210000004881 tumor cell Anatomy 0.000 description 5
- 210000001367 artery Anatomy 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000036772 blood pressure Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 238000012744 immunostaining Methods 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 206010027336 Menstruation delayed Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002424 anti-apoptotic effect Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000973 chemotherapeutic effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000011528 liquid biopsy Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 108091008104 nucleic acid aptamers Proteins 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses the specific cells in body to capture box, includes capture box, the biomarker that can be combined with specific cells is equipped in the capture box, the capture box is equipped with blood entry port and blood exports.The configuration of the present invention is simple, can carry out the early screening of tumour, the drug susceptibility of tumour, tumor load, bacterium antibiotics sensitivity etc..For the present invention without blood drawing, the cell of capture is more, at low cost, easy to operate, and efficiently, nontoxic.
Description
Technical field：
The present invention relates to the specific cells in body to capture box.
Background technology：
Cancer is still the number one killer of the mankind so far, and the newly-increased cases of cancer 14,100,000 in the whole world in 2015,8,200,000 people die of cancer
Disease.Research shows that most of tumor patient dies of metastatic tumor rather than primary tumor；Metastases are to lead to cancer death
The main reason for.By current medical level, to the infantile tumour patient that not yet shifts, there are about 80%-90% or more to cure；
But for the tumor patient in late period, survival rate is only 8-10% within 5 years.Therefore, the early detection of metastases and tumour are controlled
The monitoring of the effect of during treatment has decisive significance to the treatment of tumour.Currently, in diagnosing tumor with treatment, doctor is usual
Utilize imaging means（Such as PET-CT）Determine the lesions position of tumour, and using invasive solid tumor biopsy come
It is qualitative and parting by stages is carried out to tumour to tumour.Current this detection mode has the disadvantages that：1, it can not achieve really
The early detection of meaning；2, expensive；3, invasive inspection, it is larger to patient's injury, it cannot frequently use；4, it can not achieve
Detection in real time.
Circulating tumor cell refers to the tumour cell that blood circulation system is detached and entered from primary tumor or transfer stove,
These cells to form the potential of new neoplasm metastasis with adhering at other positions of body.Metastases are often because follow
Caused by ring tumour cell, one of indication that the discovery of circulating tumor cell can be as metastases.These heterogeneities are followed
Ring tumour cell has very strong anti-apoptotic ability, transfer incidence, chemotherapeutic efficacy, recurrence and existence with tumor patient
Time is closely related, thus as metastases, recurrence and more after important indicator.But for leucocyte, cycle
Tumour cell is considerably less, if we can extract circulating tumor cell, the currently popular slender extracellular molecule sequencing of cooperation
Technology, we can be in position, parting and the mutational site for determining that tumour in early days, and it is suitable just to be formulated in early stage in this way
Therapeutic scheme strangles cancer in rudiment；Cooperation immunostaining technique and cell count can analyze tumor load and tumour
The effect for the treatment of；Cooperation drug study can be obtained by the Drug sensitivity data of tumour, and neoplastic disease can be adjusted according to data
The therapeutic scheme of people.
The method that most commercialization CTC detectors are all made of vitro detection both at home and abroad at present, the technology of CTC is with the U.S.
The CellSearch FCM analysis instrument of johnsen diagnostic companies and the IsoFlux of Fluxion companies are representative, this two set
The standby certification for all obtaining U.S. FDA.There are many detection method of extracorporal circulatory system tumour cell, can be divided into the moon according to the method for enrichment
Property enrichment method and positive enrichment method.Principle is dependent on external blood drawing and cell separation technology, although circulating tumor cell is examined
Survey technology has application prospect very much, however technically there are still defects.Such as operate complicated, recall rate is low and by blood drawing amount
Limitation（<15ml) etc..It is limited to total blood sampling volume, the sensitivity of the detection of these external circulating tumor cell monitors is normal
Requirement cannot often be reached.
Invention content：
The object of the invention is exactly to provide the specific cells in body in order to make up the defect of prior art and capture box.The present invention
Many things can be detached from blood, but main effect is that circulating tumor cell is detached from blood.Detach circulating tumor
The purpose of cell is to carry out the liquid biopsy of early stage.
The present invention is achieved by the following technical solutions：
One specific cells in body captures box, it is characterised in that：Include capture box, be equipped in the capture box can with it is specific
The biomarker of cell combination, the capture box is equipped with blood entry port and blood exports.
Described one captures box in the specific cells of body, it is characterised in that：The blood entry port and blood outlet are upper equal
It is connected with woven hose, woven hose outer end is equipped with syringe needle, adjuster is equipped on woven hose.
Described one captures box in the specific cells of body, it is characterised in that：Blood transfusion apparatus is installed on any woven hose.
Described one captures box in the specific cells of body, it is characterised in that：The specific cells be circulating tumor cell,
Immunocyte, bacterium or the cell that can be detached from blood.
Described one captures box in the specific cells of body, it is characterised in that：The biomarker is antibody or nucleic acid
Aptamers.
Described one captures box in the specific cells of body, it is characterised in that：Biomarker passes through covalent coupling, molecule
The effects that Interaction Force or hydrogen bond power be fixed on capture box in.
Described one captures box in the specific cells of body, it is characterised in that：It captures and is equipped with gel or biomembrane, institute in box
Biomarker is stated to be fixed on gel or biomembrane.
Described one captures box in the specific cells of body, it is characterised in that：It captures and is equipped with magnetic bead in box, the biology mark
Note object is fixed on magnetic bead.
The woven hose is corollary apparatus.
There are many acquisition modes of the present invention, including artery-vein connection capture, vein-vein connection capture.Artery-
It is to be connected to artery with vein by the woven hose at both ends that vein, which is connected to acquisition mode, so that blood is entered under the promotion of blood pressure and catches
Box is obtained, to be combined with biomarker.Vein-vein connection acquisition mode is by the woven hose at both ends by vein and vein
Connection, capture box is pumped under the action of pump by blood pressure.
Capture box can have variously-shaped, specification, color and material.
The present invention can be artery and vein, can also be quiet in use, be inserted into blood vessel respectively by the needle on woven hose
Arteries and veins and vein allow blood to flow through the capture box among two needles, and capturing has and can be specifically bound with circulating tumor cell in box
Biomarker.Blood is allowed to be flowed in capture box, after the regular hour, all blood of human body can all pass through capture
Box can capture more circulating tumor cells, and need not extract blood in this way, not limited by amount for taking blood.
Requirement of the capture box for specification, color, material etc. in the present invention is few, nontoxic.
It is a kind of hollow structure to capture box, and main effect is the biology mark that storage can be specifically bound with specific cells
Remember object.As corollary apparatus, box it is of less demanding, as long as inner space is slightly a little bigger, nontoxic.It can basis
Specification, shape, the material etc. of the fixed method of cell category, biomarker of capture, the different change boxes of experiment purpose.
The effect of biomarker captured in box is combined with specific cells, is needed to secure it on magnetic bead or box
In the inside of son or box on other materials, to which specific cells are enriched with.Biomarker can be various types of
Antibody and aptamer.The specificity of biomarker need it is high a bit, but since blood volume is big, the cell of capture compared with
It is more, specificity can not also use it is too high, this is also an advantage of our invention.Biomarker can be according to the thin of capture
The type of born of the same parents, the difference of the purpose of experiment and replace the fixing means of different biomarkers and biomarker.
The present invention can realize the highly selective and high specific specific cell of capture in body.Separation is matched after the completion of capture
Covering device and capture box, after capturing box with normal saline flushing, being eluted the cell of capture using eluent can be into
Row next step analyzes.
Advantages of the present invention is：
The configuration of the present invention is simple, can carry out the early screening of tumour, the drug susceptibility of tumour, tumor load, bacterium it is anti-
Raw element sensibility etc..For the present invention without blood drawing, the cell of capture is more, at low cost, easy to operate, and efficiently, nontoxic.
Description of the drawings：
Fig. 1 is the structural diagram of the present invention.
Specific implementation mode：
Referring to attached drawing.
One specific cells capture box in body includes capture box 1, and being equipped in the capture box can be with specific cells knot
The biomarker 2 of conjunction, the capture box 1 is equipped with blood entry port and blood exports.
It is respectively connected with woven hose 3 outside the blood entry port and blood outlet, 3 outer end of woven hose is equipped with syringe needle 4, woven hose
Adjuster 5 is installed on 3.
Blood transfusion apparatus 6 is installed on any woven hose 3.
The specific cells are circulating tumor cell, immunocyte, bacterium or the cell that can be detached from blood.
The biomarker is antibody or aptamer.
Biomarker is fixed on by power the effects that covalent coupling, intermolecular interaction or hydrogen bond in capture box.
It captures and is equipped with gel or biomembrane in box 1, the biomarker is fixed on gel or biomembrane.
It captures and is equipped with magnetic bead in box 1, the biomarker is fixed on magnetic bead.
The operating process that one specific cells in body captures box is as follows：
（1）It first has to using physiological saline, by rinse inside the present invention one time, transfusion needle is directly inserted into the infusion bottle of bottle, is allowed
Physiological saline flows down naturally.The effect of this step is to allow liquid full of capture box and transfusion needle, prevents liquid gas from entering people
Body, but also capture box can be rinsed.
(2) needle in the corollary apparatus that will be connected with capture box both ends by label penetrate blood vessel respectively, if it is artery with
Vein just allows blood to be flowed in capture box.If it is vein and vein, it is necessary to start mating peristaltic pump to provide
Power.To the regular hour, so that it may to remove corollary apparatus, separation corollary apparatus and capture box, be caught with normal saline flushing
Obtain box.
(3) cell of capture is washed using cell elution reagent, then can be carried out the processing analysis of next step
, can also be enriched with again primary.Experimental purpose can be with choice experiment room culture, antibiotics sensitivity, sequencing, unicellular
The experiments such as sequencing, immunostaining, cell count and drug susceptibility.Our capture box also has transparent box body, facilitates experiment
The case where the case where member directly observes the cell of capture through box body and dyeing.
Claims (8)
1. the specific cells in body capture box, it is characterised in that：Include capture box, being equipped in the capture box can be with spy
Determine the biomarker of cell combination, the capture box is equipped with blood entry port and blood exports.
2. the one according to claim 1 specific cells in body capture box, it is characterised in that：The blood entry port and blood
Liquid is respectively connected with woven hose in outlet, and woven hose outer end is equipped with syringe needle, adjuster is equipped on woven hose.
3. the one according to claim 2 specific cells in body capture box, it is characterised in that：It is installed on any woven hose
There is blood transfusion apparatus.
4. capturing box according to any specific cells in body in claim 1-3, it is characterised in that：It is described specific
Cell is circulating tumor cell, immunocyte, bacterium or the cell that can be detached from blood.
5. the one according to claim 4 specific cells in body capture box, it is characterised in that：The biomarker is
Antibody or aptamer.
6. the one according to claim 5 specific cells in body capture box, it is characterised in that：Biomarker passes through altogether
Valence coupling, intermolecular interaction or power is fixed in capture box the effects that hydrogen bond.
7. the one according to claim 5 specific cells in body capture box, it is characterised in that：It captures and is equipped with gel in box
Or biomembrane, the biomarker are fixed on gel or biomembrane.
8. the one according to claim 5 specific cells in body capture box, it is characterised in that：It captures and is equipped with magnetic in box
Pearl, the biomarker are fixed on magnetic bead.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810934966.0A CN108761080A (en) | 2018-08-16 | 2018-08-16 | One specific cells in body captures box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810934966.0A CN108761080A (en) | 2018-08-16 | 2018-08-16 | One specific cells in body captures box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108761080A true CN108761080A (en) | 2018-11-06 |
Family
ID=63966332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810934966.0A Pending CN108761080A (en) | 2018-08-16 | 2018-08-16 | One specific cells in body captures box |
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| Country | Link |
|---|---|
| CN (1) | CN108761080A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101066477A (en) * | 2007-05-17 | 2007-11-07 | 中国人民解放军第三军医大学 | Biological artificial blood vessel capable of in vivo capturing endothelial ancestral cell |
| CN103725648A (en) * | 2013-09-28 | 2014-04-16 | 长沙赢润生物技术有限公司 | Novel CTC (circulating tumor cell) enrichment technology and preparation method of kit |
| CN103908306A (en) * | 2014-04-17 | 2014-07-09 | 山东师范大学 | Circulating tumor cell in-vivo capturing device and circulating tumor cell in-vivo capturing method on basis of indwelling needles |
| CN103940997A (en) * | 2014-03-21 | 2014-07-23 | 上海柏慧康生物科技有限公司 | Breast cancer circulating tumor cells detection system and kit |
| CN106955384A (en) * | 2017-05-04 | 2017-07-18 | 中国药科大学 | A kind of circulating tumor cell capturing device |
| CN107260226A (en) * | 2017-05-18 | 2017-10-20 | 华东医院 | A kind of internal enriching apparatus of circulating tumor cell |
| CN107338185A (en) * | 2017-08-02 | 2017-11-10 | 苏州博福生物医药科技有限公司 | The catching method of biomolecule in a kind of cell or solution |
| CN107338184A (en) * | 2017-08-02 | 2017-11-10 | 苏州博福生物医药科技有限公司 | A kind of capture sieve and device for being used to capture biomolecule in cell or solution |
-
2018
- 2018-08-16 CN CN201810934966.0A patent/CN108761080A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101066477A (en) * | 2007-05-17 | 2007-11-07 | 中国人民解放军第三军医大学 | Biological artificial blood vessel capable of in vivo capturing endothelial ancestral cell |
| CN103725648A (en) * | 2013-09-28 | 2014-04-16 | 长沙赢润生物技术有限公司 | Novel CTC (circulating tumor cell) enrichment technology and preparation method of kit |
| CN103940997A (en) * | 2014-03-21 | 2014-07-23 | 上海柏慧康生物科技有限公司 | Breast cancer circulating tumor cells detection system and kit |
| CN103908306A (en) * | 2014-04-17 | 2014-07-09 | 山东师范大学 | Circulating tumor cell in-vivo capturing device and circulating tumor cell in-vivo capturing method on basis of indwelling needles |
| CN106955384A (en) * | 2017-05-04 | 2017-07-18 | 中国药科大学 | A kind of circulating tumor cell capturing device |
| CN107260226A (en) * | 2017-05-18 | 2017-10-20 | 华东医院 | A kind of internal enriching apparatus of circulating tumor cell |
| CN107338185A (en) * | 2017-08-02 | 2017-11-10 | 苏州博福生物医药科技有限公司 | The catching method of biomolecule in a kind of cell or solution |
| CN107338184A (en) * | 2017-08-02 | 2017-11-10 | 苏州博福生物医药科技有限公司 | A kind of capture sieve and device for being used to capture biomolecule in cell or solution |
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