CN207793276U - A kind of solid-liquid diversion reaction extraction system - Google Patents
A kind of solid-liquid diversion reaction extraction system Download PDFInfo
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- CN207793276U CN207793276U CN201721898449.XU CN201721898449U CN207793276U CN 207793276 U CN207793276 U CN 207793276U CN 201721898449 U CN201721898449 U CN 201721898449U CN 207793276 U CN207793276 U CN 207793276U
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Abstract
The utility model provides a kind of solid-liquid diversion reaction extraction system, including dividing cell, equipped with storage department, reacting part and the recoverer for sealing connection successively, storage department is used for a variety of reactants of classification storage, for reacting part for reacting and solid-liquid product being centrifuged, recoverer is used for the liquid in classification storage solid-liquid product；Pumping unit, it include the injection unit being connected with storage department, and the extraction unit being connected with recoverer, injection unit centrifuge for being quantitatively injected into each reactant in storage department in reacting part according to Injection Signal, after each reactant reaction and generate the stratified mixture containing solid and liquid；Extraction unit is used to be extracted liquid classification to recoverer to complete to be separated by solid-liquid separation according to extraction signal；Control unit, for sending extraction signal to injection unit transmission Injection Signal, and to extraction unit according to control instruction.The utility model is reasonable for structure, design is simple and can realize the separation and Extraction of solidliquid mixture automatically.
Description
Technical field
The utility model belongs to medicine instrument technical field more particularly to a kind of solid-liquid diversion for extracting stem cell is anti-
Answer extraction system.
Background technology
Stem cell refers to the initial cell for having self-renewing and multi-lineage potential, is the cells of origin of body, in spy
The cell as one or more composition tissues or organ can be broken up under conditions of fixed, it is widely used in preclinical medicine and grinds
Study carefully.Self-renewing refers to the size that stem cell can maintain stem cell population by cell division；Multidirectional Differentiation refers to stem cell
With plasticity, it can be divided into different histocytes in different environments to constitute the organizer of the various complexity of body
Official.
Mescenchymal stem cell (MSCs) is one kind in stem cell, derives from the mesoderm and ectoderm of mesoderm growing early stage, because
It has the characteristics that multi-lineage potential, hematopoiesis support and promote stem cell implantation, immunoregulation and self-replacation and increasingly by
To the concern of people.Before the continuous source for seeking and improving MSCs acquisitions and method are further investigations and utilize necessity of MSCs
It carries, the separation of MSCs at present and culture lack unified approach, and common separating and extracting process is to use tissue block to original cuiture
Method or enzyme digestion obtain mesenchymal stem cells.
In general, generally requiring to add enzyme manually when obtaining mescenchymal stem cell using enzyme digestion, water, containing stem cell
The stoste etc. of tissue, manual separation waste liquid extracts stem cell in turn again after each reactant hybrid reaction is complete, this process behaviour
Make complicated, the degree of automation is low, and the addition of reactant often has error, and the extraction efficiency of stem cell is relatively low, and is extracting
Since operating environment opens in journey, stem cell is easy to by fungi or germ contamination.
Utility model content
The technical problem to be solved by the present invention is to overcome defect in the prior art, to provide a kind of solid-liquid point
Stream reaction extraction system, the separate system is reasonable for structure, design is simple and can realize liquid in stem cell extraction process automatically
Shunting injection and extraction.
In order to achieve the above objectives, the utility model adopts the following technical solution：
The utility model provides a kind of solid-liquid diversion reaction extraction system, including：
Dividing cell, equipped with storage department, reacting part and the recoverer for sealing connection successively, the storage department is deposited for classifying
A variety of reactants are stored up, the reacting part is for reacting and solid-liquid product being centrifuged, and the recoverer is for classifying
Store the liquid in the solid-liquid product；
Pumping unit includes the injection unit being connected with the storage department, and the extraction being connected with the recoverer
Portion, the injection unit are used to that each reactant in the storage department to be quantitatively injected into the reacting part according to the Injection Signal
It is interior, it is centrifuged after each reactant reaction and generates the stratified mixture containing solid and liquid；The extraction unit is used for basis and carries
The number of winning the confidence is by liquid classification extraction to the recoverer to complete to be separated by solid-liquid separation；
Control unit, for sending the Injection Signal to the injection unit according to control instruction, and to the extraction
Portion sends the extraction signal.
Preferably：The injection unit include injection pump and respectively with multiple injection electromagnetism for connecting of injection pump signal
Valve；The injection pump is set to the arrival end of the reacting part, the conducting for controlling the storage department according to the Injection Signal
State, multiple injection solenoid valves are set to the outlet end of the storage department correspondingly, to be believed according to the injection
The injection rate of number each reactant of control.
Preferably：The storage department is three, and enzyme, physiological saline are respectively equipped in three storage departments and contains stem cell
Tissue fluid, described control unit controls the enzyme, physiological saline and tissue fluid containing stem cell by the injection unit
Order of addition and additive amount.
Preferably：Solid-liquid diversion reaction extraction system further includes detection unit, the detection unit respectively with it is described
Control unit is connected with the extraction unit signal, and the detection unit is used to detect the state of the liquid, described control unit
The extraction signal is sent to the extraction unit carry out classification extraction according to the state of the liquid.
Preferably：The extraction unit includes multiple extraction electromagnetism that extraction is pumped and connect respectively with the extraction pump signal
Valve, the extraction pump is set on the outlet end of the reacting part, the conducting for controlling the recoverer according to the extraction signal
State, multiple extraction solenoid valves are set to the arrival end of the recoverer correspondingly, to be believed according to the extraction
Number classification extracting liq.
Preferably：The recoverer is two, and two recoverers are respectively used to storage aqueous solution or grease.
Preferably：The junction of the storage department and the recoverer and the reacting part is equipped with sealing device, described
Sealing device makes dynamic sealing between the storage department and the reacting part and the recoverer and the reacting part.
Further：The sealing device includes the axle bed, conduit and sealing ring for having axis hole；The axle bed is two,
Two axle beds are respectively arranged on the reacting part, and the storage department and the recoverer are any described by being arranged in respectively
Conduit in axis hole is connected with the reacting part, sealing ring built in the axis hole, and the sealing ring is matched with the conduit interference
Conjunction forms dynamic sealing.
The beneficial effects of the utility model are：
(1) first, the storage department in the utility model dividing cell can be with a variety of reactants of classified storage, and recoverer can be with
The waste liquid of classified storage different conditions, and storage department, recoverer are connected to reacting part sealing, avoid free surrounding space to entire
The pollution of reaction process；Secondly the utility model is provided with the pumping unit being connected with dividing cell and control pumping unit
The control unit of operation, the pump section of pumping unit can according to the Injection Signal that control unit is sent out will be in storage department it is each anti-
It answers object to be quantitatively injected into reacting part to be reacted and be layered, what the extraction unit of pumping unit can be sent out according to control unit carries
The number of winning the confidence is by the liquid classification extraction after reaction to recoverer to complete to be separated by solid-liquid separation；The utility model is without manually grasping
Make, device sealing can avoid the pollution of external environment, and entire extraction process of reacting all is carried out, realized under the control of the control unit
Whole-course automation, the utility model sample introduction is accurate, waste liquid can be automatically drained out, and the extraction moment for stem cell is effectively simple
Change the process of the extraction separation of traditional stem cell；
(2) injection unit of the utility model is in the case where injection pump is opened, by being correspondingly arranged with storage department outlet end
Injection solenoid valve control the injection rate of each reactant；Extraction unit is in the case where extraction pump is opened, by entering with recoverer
The liquid that the extraction solenoid valve that mouth end is correspondingly arranged generates reaction carries out classification recycling；
(3) the utility model is additionally provided with detection unit, and detection unit can pump extracted liquid condition with Detection and Extraction,
Detection unit can be video camera, and video camera is extracted the state of pump extracting liq, such as color or particle by implementing monitoring
Degree etc., shot by camera to picture be transferred into control unit, control unit judges that extract is according to liquid condition
When purpose thing (oil phase), then one of extraction solenoid valve is opened, purpose thing is extracted to corresponding recoverer；When judgement is extracted
When liquid is waste liquid (water phase), then aforementioned extraction solenoid valve is closed, another extraction solenoid valve is opened, is extracted to corresponding time
Receipts portion, to complete to generate the classification recycling of liquid after reacting；
(4) storage department of the utility model, recoverer are connected to reacting part sealing, since reacting part needs to complete centrifugation
The process of separation, thus by sealing device come realize storage department, recoverer with the dynamic sealing of reacting part, the sealing device
Can dynamic sealing be formed by the interference fit between axle bed, conduit and sealing ring with axis hole.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural schematic diagram of the utility model one embodiment.
Reference sign：
11, storage department；12, recoverer；13, reacting part；21, injection unit；211, injection pump；212, solenoid valve is injected；22
Extraction unit；221, extraction pump；222 extraction solenoid valves；3, control unit；4, detection unit；5, sealing device.
Specific implementation mode
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
The every other embodiment that field those of ordinary skill is obtained without making creative work, belongs to this practicality
Novel protected range.
It is in the description of the present invention, it should be noted that term "center", "upper", "lower", "left", "right", " perpendicular
Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
The utility model and simplifying describes for ease of description, do not indicate or imply the indicated device or element must have it is specific
Orientation, with specific azimuth configuration and operation, therefore should not be understood as limiting the present invention.In addition, term " the
One ", " second ", " third " are used for description purposes only, and are not understood to indicate or imply relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, can also be electrical connection；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, it can understand above-mentioned art with concrete condition
The concrete meaning of language in the present invention.
In addition, as long as technical characteristic involved in the utility model different embodiments disclosed below is each other
Not constituting conflict can be combined with each other.
As shown in Figure 1, the utility model provides a kind of solid-liquid diversion reaction extraction system, including：Dividing cell is equipped with
Storage department 11, reacting part 13 and the recoverer 12 of connection are sealed successively, and storage department 11 is used for a variety of reactants of classification storage, reaction
For reacting and solid-liquid product being centrifuged, recoverer 12 is used for the liquid in classification storage solid-liquid product in portion 13
Body；Pumping unit includes the injection unit 21 being connected with storage department 11, and the extraction unit 22 being connected with recoverer 12, note
Enter portion 21 to be used to that each reactant in storage department 11 to be quantitatively injected into reacting part 13 according to Injection Signal, each reactant reaction
It centrifuges afterwards and generates the stratified mixture containing solid and liquid；Extraction unit 22 is used to be proposed liquid classification according to extraction signal
It takes to recoverer 12 to complete to be separated by solid-liquid separation；Control unit 3, for sending Injection Signal to injection unit according to control instruction, with
And send extraction signal to extraction unit 22.
Storage department 11 in the utility model dividing cell can be classified with a variety of reactants of classified storage, recoverer 12
The waste liquid of different conditions is stored, and storage department 11, recoverer 12 are connected to the sealing of reacting part 13, avoid external environment to whole
The pollution of a reaction process；Secondly the utility model is provided with the pumping unit being connected with dividing cell and control pumping is single
The control unit of member operation, the injection unit 21 of pumping unit can be according to the Injection Signals that control unit 3 is sent out by storage department 11
Interior each reactant, which is quantitatively injected into reacting part 13, to be reacted and is layered, and the extraction unit 22 of pumping unit can be according to control
The extraction signal that unit 3 is sent out is by the liquid classification extraction after reaction to recoverer 12 to complete to be separated by solid-liquid separation；The utility model
Without manual hand manipulation, device sealing can avoid the pollution of external environment, and entire extraction process of reacting is all in control unit 3
Control is lower to be carried out, and realizes whole-course automation, the utility model sample introduction is accurate, and waste liquid can be automatically drained out, and is used for stem cell
The extraction moment effectively simplify traditional stem cell extraction separation process.
Wherein injection unit 21 is set between storage department 11 and reacting part 13, includes the injection pump set on 12 arrival end of reacting part
211 and multiple injection solenoid valves 212 being arranged in parallel for being connect respectively with 211 signal of injection pump, wherein each injection electromagnetism
Valve 212 is set to the outlet end of storage department 11 correspondingly, and injection pump 211 is used to control leading for storage department 11 according to Injection Signal
Logical state injects solenoid valve 212 to control the injection rate of each reactant according to Injection Signal.
The utility model is used for the separation and Extraction of solid-liquid product, such as the separation and Extraction of stem cell on medical domain,
When for separating and extracting stem cells, storage department 11 is preferably three, and three storage departments 11 are arranged in parallel, each storage department 11
It is inside respectively equipped with enzyme, physiological saline and the tissue fluid containing stem cell, after the energization of control unit 3 receives operating instruction, to note
Enter pump 211 and send Injection Signals, while respectively to corresponding to containing physiological saline, enzyme or the storage department of liquid containing stem cell tissue 11
Inject solenoid valve 212 send Injection Signal, after injection pump 211 receives Injection Signal, to storage department 11 act on, when with storage department
After 11 corresponding injection solenoid valves 212 receive Injection Signal, the corresponding solenoid valve 212 that injects is in the conduction state, this moment should
Storage department 11 and reacting part 13 are fully on, and the reactant in the storage department 11 injects reacting part under the action of injection pump 211
In 13, and the injection solenoid valve 212 that Injection Signal is not received by under other storage departments 11 is closed, not with reacting part
It is connected between 13, when the reactant in the storage department 11 reaches certain injection rate, control unit 3 is again to corresponding to it
It injects solenoid valve 211 and sends instruction, which is closed after receiving instruction, and then realizes determining for reactant
Amount injection, in other storage departments 11 injection of reactant be also in this way, certainly according to the signal instruction of control unit 3, also can be more
A storage department 11 is injected into reacting part 13 simultaneously, after reactant is injected, each reactant in the reacting part 13 into
Row concussion mixing reaction, extraction link is entered after centrifugation layering.Injection pump and the preferred wriggling of extraction pump in the present embodiment
Pump.
Extraction unit 22 is set between reacting part 13 and recoverer 12, includes the extraction pump 221 set on 13 outlet end of reacting part
And the multiple extraction solenoid valves 222 being arranged in parallel being connect respectively with extraction 221 signals of pump, wherein each extraction solenoid valve
222 are set to the arrival end of recoverer 12, conducting of the extraction pump 221 for controlling recoverer 12 according to extraction signal correspondingly
State, extraction solenoid valve 222 is to according to extraction Modulation recognition extracting liq.Preferably, the recoverer 12 of the present embodiment is
Two, two recoverers 12 are respectively used to storage water phase or oil phase.
Unit 3 sends extraction signal to the extraction process of the utility model to extraction pump 221 in order to control, and extraction pump 221 receives
Reacting part 13 is acted on after to extraction signal, while control unit 2 sends extraction signal to specified extraction solenoid valve 222, specifies
Extraction solenoid valve 222 receives conducting recoverer 12 and extraction pump 221 after extraction signal, and the aqueous solution in lower layer will be first
It first extracts to the recoverer 12 corresponding to specified extraction solenoid valve 222, when control unit 3 receives the water-soluble of detection signal transmission
After the instruction that liquid extraction is completed, signal will be sent to specified extraction solenoid valve 222, extraction solenoid valve 222 is specified to close, while to
Another extraction solenoid valve 222 sends extraction signal, and another extraction solenoid valve 222 completes the extraction of oil phase according to same process,
And then realize the classification recycling of liquid, and required solid (stem cell) is left in reacting part 13, final realize is separated by solid-liquid separation.
Storage department 11, recoverer 12 are connected to the sealing of reacting part 13 in the present embodiment, since reacting part 13 has needed
At the process of centrifugation, thus by sealing device 5 come realize storage department 11, recoverer 12 with the dynamic of reacting part 13 it is close
Envelope, which includes the axle bed, conduit and sealing ring for having axis hole；Axle bed is two, and two axle beds are respectively arranged on reaction
In portion 13, storage department 11 and recoverer 12 are connected by the conduit being arranged in any axis hole with reacting part 13 respectively,
The catheter tip usually connected with recoverer 12 is away from 0.2mm is no more than at a distance from reacting part bottom wall, this is because conduit can turn
Dynamic setting is on reactor, without moving in the axial direction, when distance setting is no more than 0.2mm, it is ensured that waste liquid quilt
It removes completely, and without the insertion depth of adjustment conduit, to realize the full automation of the present apparatus；In addition it injection unit 21 and carries
Take portion 22 to be set to accordingly on the conduit corresponding to storage department 11 and recoverer 12, sealing ring built in axis hole, sealing ring with lead
Pipe is interference fitted to form dynamic sealing.When running at high speed under drive of the reacting part 13 in motor etc., conduit with it is anti-
It answers the dynamic sealing between portion 13 to be effectively guaranteed the reactant in the present apparatus and cross contamination does not occur with exterior space.
The conduit between recoverer and reacting part 13 is preferably transparent pipe in the present embodiment, in addition extraction pump 221 with
The detection unit 4 of one liquid condition extracted to Detection and Extraction pump 221 of setting, the detection between extraction solenoid valve 222
Unit 4 can be video camera, and video camera is extracted the state of 221 extracting liqs of pump, such as color or particle by implementing monitoring
Degree etc., shot by camera to picture be transferred into control unit 3, control unit 3 is extracted liquid according to liquid condition judgement
Body then opens one of extraction solenoid valve 221 if grease, and grease is extracted to corresponding recoverer 12；When judging extracting liq
For aqueous solution when, then close aforementioned extraction solenoid valve 222, open another extraction solenoid valve 222, extracted to corresponding recycling
Portion 12, to complete to generate the classification recycling of liquid after reacting.
The above is only the specific embodiments of the utility model, and but protection scope of the utility model is not limited thereto,
Any one skilled in the art within the technical scope disclosed by the utility model, the variation that can readily occur in or replaces
It changes, should be covered within the scope of the utility model.Therefore, the scope of protection of the utility model should be with claim
Subject to protection domain.
Claims (8)
1. a kind of solid-liquid diversion reacts extraction system, which is characterized in that including：
Dividing cell, equipped with storage department, reacting part and the recoverer for sealing connection successively, the storage department is more for classification storage
Kind reactant, for the reacting part for reacting and solid-liquid product being centrifuged, the recoverer is used for classification storage
Liquid in the solid-liquid product；
Pumping unit includes the injection unit being connected with the storage department, and the extraction unit being connected with the recoverer, institute
State injection unit for each reactant in the storage department to be quantitatively injected into the reacting part according to the Injection Signal, respectively
It is centrifuged after reactant reaction and generates the stratified mixture containing solid and liquid；The extraction unit is used for according to extraction signal
By liquid classification extraction to the recoverer to complete to be separated by solid-liquid separation；
Control unit, for being sent out to the injection unit transmission Injection Signal, and to the extraction unit according to control instruction
Send the extraction signal.
2. solid-liquid diversion according to claim 1 reacts extraction system, which is characterized in that the injection unit includes injection pump
And the multiple injection solenoid valves being connect respectively with the injection pump signal；The injection pump is set to the entrance of the reacting part
End, the conducting state for controlling the storage department according to the Injection Signal, multiple injection solenoid valves are one-to-one
It is set to the outlet end of the storage department, to control the injection rate of each reactant according to the Injection Signal.
3. solid-liquid diversion according to claim 1 reacts extraction system, which is characterized in that the storage department is three, three
Enzyme, physiological saline and the tissue fluid containing stem cell are respectively equipped in a storage department, described control unit passes through the injection
Portion controls the order of addition and additive amount of the enzyme, physiological saline and the tissue fluid containing stem cell.
4. solid-liquid diversion according to claim 1 reacts extraction system, which is characterized in that the solid-liquid diversion reaction extraction
System further includes detection unit, and the detection unit is connect with described control unit and the extraction unit signal respectively, the inspection
The state that unit is used to detect the liquid is surveyed, described control unit sends institute according to the state of the liquid to the extraction unit
It states extraction signal and carries out classification extraction.
5. solid-liquid diversion according to claim 1 reacts extraction system, which is characterized in that the extraction unit includes that extraction pumps
And the multiple extraction solenoid valves being connect respectively with the extraction pump signal, the extraction pump is set on the outlet of the reacting part
End, the conducting state for controlling the recoverer according to the extraction signal, multiple extraction solenoid valves are one-to-one
It is set to the arrival end of the recoverer, to according to the extraction Modulation recognition extracting liq.
6. solid-liquid diversion according to claim 1 reacts extraction system, which is characterized in that the recoverer is two, two
A recoverer is respectively used to storage aqueous solution or grease.
7. solid-liquid diversion according to claim 1 reacts extraction system, which is characterized in that the storage department and the recycling
The junction of portion and the reacting part is equipped with sealing device, the sealing device make the storage department and the reacting part and
Dynamic sealing between the recoverer and the reacting part.
8. solid-liquid diversion according to claim 1 reacts extraction system, which is characterized in that the sealing device includes having
Axle bed, conduit and the sealing ring of axis hole；The axle bed is two, and two axle beds are respectively arranged on the reacting part, described
Storage department and the recoverer are connected by the conduit being arranged in any axis hole with the reacting part respectively, the axis
Sealing ring built in hole, the sealing ring are interference fitted to form dynamic sealing with the conduit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721898449.XU CN207793276U (en) | 2017-12-29 | 2017-12-29 | A kind of solid-liquid diversion reaction extraction system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721898449.XU CN207793276U (en) | 2017-12-29 | 2017-12-29 | A kind of solid-liquid diversion reaction extraction system |
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| Publication Number | Publication Date |
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| CN207793276U true CN207793276U (en) | 2018-08-31 |
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| CN201721898449.XU Expired - Fee Related CN207793276U (en) | 2017-12-29 | 2017-12-29 | A kind of solid-liquid diversion reaction extraction system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988706A (en) * | 2017-12-29 | 2019-07-09 | 肖锷 | A kind of centrifugal bottle and stem cell are classified extraction element |
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2017
- 2017-12-29 CN CN201721898449.XU patent/CN207793276U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988706A (en) * | 2017-12-29 | 2019-07-09 | 肖锷 | A kind of centrifugal bottle and stem cell are classified extraction element |
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