CN207948637U - The drosophila breeding apparatus that multicell combined type drosophila feed intake accurately measures - Google Patents

The drosophila breeding apparatus that multicell combined type drosophila feed intake accurately measures Download PDF

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Publication number
CN207948637U
CN207948637U CN201820272211.4U CN201820272211U CN207948637U CN 207948637 U CN207948637 U CN 207948637U CN 201820272211 U CN201820272211 U CN 201820272211U CN 207948637 U CN207948637 U CN 207948637U
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China
Prior art keywords
drosophila
box cover
feed intake
box
diameter
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Expired - Fee Related
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CN201820272211.4U
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Chinese (zh)
Inventor
吴奇
杨明耀
李克勤
高月身
崔浪
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Sichuan Agricultural University
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Sichuan Agricultural University
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Abstract

The drosophila breeding apparatus that the utility model multicell combined type drosophila feed intake accurately measures, be provided on the porous plate in transparent acrylic shell it is multiple put cartridge apertures, put and be placed with feed intake determination box in cartridge apertures;The diameter of the cylindrical shape box body of feed intake determination box (5) is less than the diameter for putting cartridge apertures, and the diameter of box cover is more than the diameter for putting cartridge apertures, and cylindrical shape box body lower opening is covered by one piece of round breathable nylon cloth.Box cover lower part is containing there are one ring-shaped rubber sealing rings.One or more short sleeves for passing through box cover are fixedly installed in box cover, the insertion of tight fit is cased with capillary to two openings of the short sleeve in the inside and outside two sides of box cover, the short sleeve respectively, and the interior diameter of the capillary is 0.55mm.Advantage:Not only have the function of to drosophila for food, but also naturally have the function of accurate measurement, carry out feed intake very easy in the drosophila long period can accurately be measured using the present apparatus.

Description

The drosophila breeding apparatus that multicell combined type drosophila feed intake accurately measures
Technical field
The utility model belongs to the drosophila breeding apparatus of genetics experiments, especially can accurately be measured to drosophila feed intake Breeding apparatus.
Background technology
Sichuan Agricultural University 201620021357.2《A kind of conical flask for raising drosophila》It discloses one kind and avoiding fruit Fly easily escape and easily by asphyxia by external pressure on the chest and abdomen the problem of taper raising drosophila bottle so that operation is calm calm, reduces operation difficulty, simultaneously Anesthetic dose can be preferably controlled, avoids anaesthetizing the adverse effect for generating drosophila.
Northeast Agricultural University 201510654755.8《With the drosophila breeding apparatus being automatically separated with collecting function》It is open It is a kind of to lure fly collector using lamp, drosophila is trapped in collection fly pipe, drosophila is automatically separated, then under air-breathing pumping action, from Drosophila is collected into fly pipe by dynamic fly suction device, to reach the scene for not allowing operating personnel to be clearly apparent Growth of Drosophila Melanogaster simultaneously The purpose of ripe drosophila efficiently can be automatically collected, is made simply, operability is strong, of low cost, with obvious effects.
Drosophila is a kind of common model organism in biological study, and in Nutritional studies, drosophila is commonly used for grinding Study carefully influence of the Different Nutrition condition to physiological status.In such research, the measurement to the practical feed intake of drosophila is a necessity Project, but still lack a kind of accurate, efficient drosophila at present and search for food amount determining device.Drosophila feed intake is accurately measured, There is important meaning in drosophila life cycle research, but existing drosophila breeding apparatus has no what it accurately measured drosophila feed intake Breeding apparatus.
Invention content
The utility model provides a kind of multiple-unit and is combined into the unified external condition for providing cultivation, and can be to each list Drosophila feed intake in member carries out the drosophila breeding apparatus accurately measured for a long time.
The structure of the utility model:
The drosophila breeding apparatus that multicell combined type drosophila feed intake accurately measures, including transparent acrylic shell 1, lower section There is aqua storage tank 2 below acrylic shell 1, keep flat on aqua storage tank 2 equipped with porous plate 3, it is identical to be provided with size on porous plate 3 It is multiple to put cartridge apertures 4, it is characterised in that:Putting for porous plate 3 is placed with feed intake determination box 5 in cartridge apertures 4;
The box body that feed intake determination box 5 includes cylindrical plastic pipe, is made of breathable nylon cloth 7 in the adhesion of plastics bottom of the tube 6, box cover 8,8 lower section of box cover are equipped with the ring-shaped rubber sealing ring 9 being connected directly with box cover 8;The diameter of box body 6, which is less than, puts cartridge apertures 4 Diameter, the diameter of box cover 8 is more than the diameter for putting cartridge apertures 4;When covering box cover 8 on box body 6, rubber seal 9 can protect It demonstrate,proves box cover 8 and box body 6 closely connects, be unlikely to fall off.
It is fixedly installed one or more short sleeves 10 for passing through box cover 8, two openings point of the short sleeve 10 in box cover 8 The insertion of tight fit is not cased with capillary 11 in the inside and outside two sides of box cover 8, the short sleeve 9, and the interior diameter of the capillary 11 is 0.55mm。
Multiple feed intake determination boxes 5 can be placed on one block of porous plate 3, i.e., the breeding apparatus that an appetite accurately measures can Multiple feed intake determination boxes 5 are placed simultaneously, carrying out feed intake to the drosophila in multiple feed intake determination boxes 5 accurately measures.
Aqua storage tank 2 is used to provide the drosophila in multiple feed intake determination boxes 5 temperature and humidity etc. for being suitble to growth, breeding External condition.
Cylindrical plastic box body 6 and its breathable nylon cloth 7 of bottom, box cover 8 provide for the drosophila of life wherein can be saturating The living space of gas.
The diameter of cylindrical plastic box body 6 is less than the diameter for putting cartridge apertures 4, so that box body 6 is passed through and puts cartridge apertures 4, cylindrical shape modeling Body 6 can put cartridge apertures 4 in the following, and box cover 8 putting above cartridge apertures 4;When covering box cover 8 on box body 6, it is connected to box cover Under rubber seal 9 can ensure that box cover 8 and box body 6 closely connect, and be unlikely to fall off, make the drosophila in box body 6 will not It runs out of.
The interior diameter 0.55mm of capillary 11 makes capillary 11 to there is the fluid drosophila food hairiness spy of certain viscosity With, and the fluid drosophila food of certain viscosity can under the effect of gravity, be made to be moved down at 11 lower ending opening of capillary, for plastics Drosophila in box body 6 is edible.
It can carry out the principle that drosophila feed intake accurately measures:Due to the gravity of liquid food, make the stream of certain viscosity Body drosophila food can be moved down at 11 lower ending opening of capillary, and due to liquid tension, make fluid drosophila food not to dirty Go out capillary 11, drosophila can be in the food in feeding capillary 11 at 11 lower ending opening of capillary.When drosophila can be in capillary 11 After food at lower ending opening in feeding capillary 11, the drosophila food in capillary 11 flows downwardly into 11 lower end of capillary and opens again At mouthful.The capillary 11 of the diameter not only has the function of to drosophila for food, but also naturally has the function of accurate measurement, So there is the feed intake determination box 5 of capillary 11 to have the function of that drosophila feed intake accurately measures in this breeding apparatus.
It is equipped with elastic packing pipe lid 12 on 11 open end of capillary of 8 outside of box cover.Elastic packing pipe lid 12 is sealed in 11 upper end of capillary, can stop the capillary 11 in box drosophila supply food, to it is various temporarily cease feeding test operation it is non- It is often convenient.
The advantages of the utility model:The capillary of this interior diameter not only has the function of to drosophila for food, but also naturally Have the function of accurate measurement, so have in this breeding apparatus capillary feed intake determination box have drosophila feed intake it is accurate The function of measurement.Elastic packing pipe lid is sealed in capillary upper end, can stop this and supply food to drosophila in box, to various temporary It is very convenient to stop feeding test operation.It can be to carry out feed intake essence very easy in the drosophila long period using the present apparatus Really measure.
Description of the drawings
Fig. 1 is that the outer casing upper cover plate of the utility model opens transparent effect schematic diagram;
Fig. 2 is the feed intake determination box dimensional structure diagram of the utility model;
Fig. 3 is the schematic diagram of the feed intake determination box structure transparent effect of the utility model;
Fig. 4 is the stereoscopic effect schematic diagram that the feed intake determination box box cover of the utility model is opened;
Fig. 5 is the feed intake determination box box cover and box body exploded perspective effect diagram of the utility model;
Fig. 6 is the feed intake determination box box cover dimensional structure diagram of the utility model;
In figure 1 be sub- shell, 2 be aqua storage tank, 3 be porous plate, 4 be put cartridge apertures, 5 be feed intake determination box, 6 be box body, 7 It is breathable nylon cloth, 8 be box cover, 9 be rubber seal, 10 be short sleeve, 11 be capillary, 12 is elastic packing pipe lid.
Specific implementation mode
Embodiment 1,
Such as the breeding apparatus that Fig. 1-6, multicell combined type drosophila feed intake are accurately measured, including transparent acrylic shell 1, There is aqua storage tank 2 below lower section acrylic shell 1, keep flat on aqua storage tank 2 equipped with porous plate 3, size phase is provided on porous plate 3 Together multiple put cartridge apertures 4, and putting for porous plate 3 is placed with feed intake determination box 5 in cartridge apertures 4;
Feed intake determination box 5 includes cylindrical plastic box body 6, sticks in the ventilative Buddhist nun of circle at plastic box body lower opening The ring-shaped rubber sealing ring 9 being connected directly with box cover below imperial cloth 7, box cover 8 and box cover, the diameter of box body 6, which is less than, puts cartridge apertures 4 Diameter, the diameter of box cover 8 is more than the diameter for putting cartridge apertures 4, when covering box cover 8 on box body 6, the ring-shaped rubber of 8 lower section of box cover Sealing ring 9 can closely connect box cover 8 and box body 6;
It is fixedly installed one or more short sleeves 10 for passing through box cover 8, two openings point of the short sleeve 10 in box cover 8 The insertion of tight fit is not cased with capillary 11 in the inside and outside two sides of box cover 8, the short sleeve 10, the interior diameter of the capillary 11 For 0.55mm.
It is equipped with elastic packing pipe lid 12 on 11 open end of capillary of 8 outside of box cover.
Specific implementation method step:
1. measure drosophila feed intake, 10 drosophilas are packed into feed intake determination box, covers and does not assign capillary glass tube Box cover 8.Due to the effect of the rubber seal 9 of 8 lower section of box cover, box cover 8 can be closely coupled with box body 6;Due to cylindrical shape 6 lower opening of plastic box body is covered by breathable nylon cloth 7, therefore 6 inner air and outer air of box body is negotiable, ensure that drosophila normally exhales It inhales.
2. 11 one end of capillary glass tube is put into the fluid nutrient medium configured in advance, due to capillary phenomenon, liquid meeting It is full of entire capillary 11 quickly.When liquid is full of entire capillary 11, capillary 11 is taken out, assigns into feed intake measurement In short sleeve 10 in the circular lid 8 of box 5, about 5mm is stretched out in lower section, this is experimental group.
3. being used as blank control, while preparing the same fluid nutrient medium of another group of perfusion but not filling the feed intake measurement of drosophila Box 5, so as to the evaporation capacity of fluid nutrient medium during experiment with computing.
4. mounted feed intake determination box experimental group and control group then, are placed in rectangular large circular hole slab 3 Circular hole 4 in, due to feed intake determination box 58 diameter of circular cover be slightly larger than 4 diameter of circular hole slab circular hole, feeding measure Determining box can be fixed on circular hole slab 3 and be unlikely in the screw clamp of whereabouts.
5. it is last, appropriate tap water will be added in sink, is put into rectangular box, covers lateral hatch and upper cover plate.This When, start timing, opened upper cover plate every about 12 hours and take out feed intake determination box 5, with ruler measurement experiment group and blank The liquid-consumed height of control group capillary tube, you can the practical feed intake of drosophila is calculated.

Claims (2)

1. the drosophila breeding apparatus that multicell combined type drosophila feed intake accurately measures, including transparent acrylic shell (1), lower section There is aqua storage tank (2) below acrylic shell (1), keep flat equipped with porous plate (3) on aqua storage tank (2), porous plate is provided on (3) Size is identical multiple to put cartridge apertures (4), it is characterised in that:Putting in cartridge apertures (4) for porous plate (3) is placed with feed intake determination box (5);
The box body that feed intake determination box (5) includes cylindrical plastic pipe, is made of breathable nylon cloth (7) in the adhesion of plastics bottom of the tube (6), box cover (8), box cover (8) lower section are equipped with the ring-shaped rubber sealing ring (9) being connected directly with box cover (8);The diameter of box body (6) Less than the diameter for putting cartridge apertures (4), the diameter of box cover (8) is more than the diameter for putting cartridge apertures (4);
One or more short sleeves (10) for passing through box cover (8) are fixedly installed in box cover (8), two of the short sleeve (10) open The insertion of tight fit is cased with capillary (11), the capillary to mouth in the inside and outside two sides of box cover (8), the short sleeve (9) respectively (11) interior diameter is 0.55mm.
2. the drosophila breeding apparatus that multicell combined type drosophila feed intake according to claim 1 accurately measures, feature It is, " is being equipped with elastic packing pipe lid (12) on capillary (11) open end outside box cover (8).
CN201820272211.4U 2018-02-08 2018-02-08 The drosophila breeding apparatus that multicell combined type drosophila feed intake accurately measures Expired - Fee Related CN207948637U (en)

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CN201820272211.4U CN207948637U (en) 2018-02-08 2018-02-08 The drosophila breeding apparatus that multicell combined type drosophila feed intake accurately measures

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109892292A (en) * 2019-03-27 2019-06-18 上海市东方医院(同济大学附属东方医院) Drug delivery device and drug delivery method for screening drugs by using drosophila
CN110915765A (en) * 2019-12-19 2020-03-27 四川农业大学 Drosophila feed intake detection method
CN115316345A (en) * 2022-07-20 2022-11-11 山东省蚕业研究所 Device for determining bumblebee food intake tendency and food intake

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109892292A (en) * 2019-03-27 2019-06-18 上海市东方医院(同济大学附属东方医院) Drug delivery device and drug delivery method for screening drugs by using drosophila
CN110915765A (en) * 2019-12-19 2020-03-27 四川农业大学 Drosophila feed intake detection method
CN110915765B (en) * 2019-12-19 2021-06-25 四川农业大学 Drosophila feed intake detection method
CN115316345A (en) * 2022-07-20 2022-11-11 山东省蚕业研究所 Device for determining bumblebee food intake tendency and food intake
CN115316345B (en) * 2022-07-20 2023-09-22 山东省蚕业研究所 Device for measuring feeding tendency and feeding amount of bumblebees

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Granted publication date: 20181012

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