CN106069776B - The aseptic culture device of multiple condition of culture can be monitored, regulated and controled automatically - Google Patents
The aseptic culture device of multiple condition of culture can be monitored, regulated and controled automatically Download PDFInfo
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- CN106069776B CN106069776B CN201610595893.8A CN201610595893A CN106069776B CN 106069776 B CN106069776 B CN 106069776B CN 201610595893 A CN201610595893 A CN 201610595893A CN 106069776 B CN106069776 B CN 106069776B
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- culture apparatus
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- main body
- apparatus main
- catch basin
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- 230000001105 regulatory effect Effects 0.000 title claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 63
- 239000002351 wastewater Substances 0.000 claims abstract description 27
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 25
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000001301 oxygen Substances 0.000 claims abstract description 18
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 12
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 12
- 230000000721 bacterilogical effect Effects 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- 238000003475 lamination Methods 0.000 claims description 3
- 229920002521 macromolecule Polymers 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 17
- 230000008635 plant growth Effects 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 2
- 238000012544 monitoring process Methods 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 description 15
- 238000012360 testing method Methods 0.000 description 11
- 229960004424 carbon dioxide Drugs 0.000 description 9
- 230000012010 growth Effects 0.000 description 6
- 239000007789 gas Substances 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000011169 microbiological contamination Methods 0.000 description 3
- -1 salt ion Chemical class 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000012376 hot air sterilization Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 description 1
- 102000010637 Aquaporins Human genes 0.000 description 1
- 108010063290 Aquaporins Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229910002090 carbon oxide Inorganic materials 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 235000006286 nutrient intake Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Hydroponics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to can monitor automatically, regulate and control the aseptic culture device of multiple condition of culture, mainly include culture apparatus main body, water inlet, delivery port, catch basin, wastewater trough, band bacteriological filtration film water guide interface tube, multiplex roles pulsating pump, water guide interface tube, CPU microprocessor, oxygen/carbon dioxide monitor, the detecting instruments such as temp-sensitive sensor, PH instrument, pressure sensor, ion concentration detector and humidity sensor are connected by pickup wire paths with CPU microprocessor respectively, catch basin and wastewater trough are connected with culture apparatus main body jointly, connected by multiplex roles pulsating pump with water conduction hose, water conduction hose connects filtration sterilization device, ensure gnotobasis in culture apparatus, it is whether normal with continuous monitoring plant growth environment, ensure that the PH of the nutrient solution in catch basin is normal.The automation of culture can be achieved in the present invention, and long-term cultivation aseptic seedling is simple to operate.
Description
Technical field
The invention belongs to field of plant tissue culture, and in particular to a kind of culture apparatus, more particularly to one kind can be automatic
Monitor, regulate and control the aseptic culture device of multiple condition of culture.
Background technology
Plant Tissue Breeding is different from conventional organization culture technique, and test tube seedling needs frequently more to renew culture medium, and test tube
Most of seedling is no offspring, could be transplanted only after taking root.Test tube seedling take root solid medium by medium component,
The puzzlement of the various factors such as plant hormone, illumination, and test tube seedling whether preserves to reserve seed for planting and is also to continue with lower step experiment or production, all
Need to survive under aseptic conditions for quite a long time.So culture environment is closed, the moisture of culture medium is kept, delays test tube
The growth of seedling and while reduce nutrient consumption in the temperature of conventional tissue culture room, illumination condition can also normal growth, this is just needed
Want indispensable condition of culture and culture technique.But conventional tissue culture operation will frequently change culture medium, change the mistake of culture medium
The main reason for there is the possibility of microbiological contamination in journey, this is also test tube seedling microbiological contamination, moisture, temperature, illumination needed for Aseptic Seedling Growth etc.
Necessary condition need it is stable and sterile, but in incubation these external environments often occur it is unstable.
The content of the invention
For technical problem existing for Plant Tissue Breeding, can be monitored automatically the invention provides one kind, regulate and control it is multiple
The aseptic culture device of condition of culture, the sterile culture of test tube seedling can be realized, reduce and change incubation times automatic watering function, avoid
Test tube seedling microbiological contamination, and the problems such as test tube seedling is withered caused by water shortage.
It is that can automatically monitor, regulate and control multiple condition of culture that the present invention, which solves the technical scheme that its technical problem uses,
Aseptic culture device, including：Water inlet, delivery port, catch basin, wastewater trough, CPU microprocessor, band bacteriological filtration film water guide interface tube,
Multiplex roles pulsating pump, water guide interface tube, oxygen/carbon dioxide concentration meter, temp-sensitive sensor, PH instrument, pressure sensor, ion are dense
Spend detector, humidity sensor, dividing plate, nursery sponge, culture apparatus main body, culture apparatus lid, pickup wire paths, water guide
Passage and waterflood path, catch basin are provided with water inlet, and wastewater trough is provided with delivery port, and multiplex roles pulsating pump is filtered by band respectively
Mycoderm water guide interface tube is connected with the water conduction hose in waterflood path and conduit pipe, multiplex roles pulsating pump by circuit with
CPU microprocessor is connected, culture apparatus main body have at 2/3rds away from bottleneck on cylindrical bottle body wall a circle projection with
Dividing plate is held up, nursery sponge is laid on dividing plate, and culture apparatus main body is connected with culture apparatus lid by spiral lamination, catch basin
It is connected with culture apparatus main body with wastewater trough, is connected by multiplex roles pulsating pump with water conduction hose jointly, water conduction hose connects
Connect filtration sterilization device, ensure gnotobasis in culture apparatus, culture apparatus bottom is equipped with ion concentration detector, in nursery sponge
Equipped with humidity sensor, temp-sensitive sensor, oxygen/carbon dioxide concentration meter, bottom are housed in culture apparatus body top space
Provided with ion concentration detector, the bottom of catch basin and wastewater trough is respectively provided with PH instrument and pressure sensor, oxygen/carbon dioxide
Concentration meter, temp-sensitive sensor, PH instrument, pressure sensor, ion concentration detector, humidity sensor pass through sensor line respectively
Passage is connected with CPU microprocessor.
Described culture apparatus main body, catch basin and wastewater trough is made up of macromolecule PC materials.Culture apparatus main body is 3 L
Cylinder, the semicircle cuboid that catch basin and wastewater trough are integrated with culture apparatus main body.Uniform aperture is 2 mm on dividing plate
Hole.
Culture apparatus main body forms closed environment after being tightened with the rotation of culture apparatus lid, after being sterilized by hot-air sterilization
Form the gnotobasis of test tube seedling growth, realize after arrange parameter pour water, water outlet automation, temperature in automatic detection culture apparatus
Degree, humidity, oxygen and gas concentration lwevel, and the PH of nutrient solution, salt ion composition.
Culture apparatus main body away from bottleneck 2/3rds at by uniform aperture be divided into two parts, dividing plate for the dividing plate in 2 mm holes
One layer of nursery sponge is covered with above to deposit test tube seedling, the nutrient solution of stored underneath plant growth.Catch basin mainly stores nothing
Bacteria culture fluid, it is connected to form sterile waterflood path with bacteriological filtration film water guide interface tube spiral by multiplex roles pulsating pump, by water filling
Nutrient solution is pumped into culture apparatus main body by the water conduction hose of passage, is poured water.Wastewater trough mainly passes through detecting system
When ion concentration detector detects that salt ion composition is not enough to supply plant continued growth in the nutrient solution of culture apparatus bottom, by
Multiplex roles pulsating pump is connected to form water guide path with water guide interface tube spiral, passes through training of being given up derived from the water conduction hose of conduit pipe
Nutrient solution is collected into wastewater trough.Described physical and chemical index detecting system predominantly detect including temperature in culture apparatus, humidity, oxygen and
Gas concentration lwevel, and the signal collector module of the PH of nutrient solution, salt ion composition pass through in pickup wire paths
Each circuit is connected into CPU microprocessor.CPU microprocessor carries data processing module, automatic control module, digital control panel
And warning device.Multiplex roles pulsating pump is connected with CPU microprocessor, when the humidity sensor under nursery sponge produces humidity not
During enough signals, CPU microprocessor produces water filling signal, and nutrient solution is passed through band bacteriological filtration film water conduction hose and connect by multiplex roles pulsating pump
Mouth injects culture apparatus main body with germ-free condition；Ion signal sensor generation ion concentration in culture apparatus main body, which is not inconsistent, to be set
When determining parameter, CPU microprocessor produces waste liquid sending out signals, and multiplex roles pulsating pump will be useless by the water conduction hose in water guide pipeline
Liquid exports.Described alarm is to carry device in CPU microprocessor system, when culture medium pH value in culture apparatus, temperature, wet
Degree, oxygen and gas concentration lwevel produce alarm when not meeting set value.
The invention has the advantages that long-term cultivation aseptic seedling, simple to operate, the automation of culture is realized.
Brief description of the drawings：
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the section of structure schematic diagram of the present invention.
In figure：1st, water inlet, 2, delivery port, 3, catch basin, 4, wastewater trough, 5, CPU microprocessor, 6, band bacteriological filtration
Film water guide interface tube, 7, multiplex roles pulsating pump, 8, water guide interface tube, 9, oxygen/carbon dioxide concentration meter, 10, temperature sensitive biography
Sensor, 11, PH instrument, 12, pressure sensor, 13, ion concentration detector, 14, humidity sensor, 15 dividing plates, 16, educate
Seedling sponge, 17, culture apparatus main body, 18, culture apparatus lid, 19, pickup wire paths, 20, conduit pipe, 21, note
Aquaporin.
Embodiment
The invention will be further described with reference to the accompanying drawings and examples：As illustrated in fig. 1 and 2,
The aseptic culture device that can automatically monitor, regulate and control multiple condition of culture of the present invention, including：Water inlet 1, water outlet
Mouth 2, catch basin 3, wastewater trough 4, CPU microprocessor 5, band bacteriological filtration film water guide interface tube 6, multiplex roles pulsating pump 7, water guide interface tube
8th, oxygen/carbon dioxide concentration meter 9, temp-sensitive sensor 10, PH instrument 11, pressure sensor 12, ion concentration detector 13, humidity
Sensor 14, dividing plate 15, nursery sponge 16, culture apparatus main body 17, culture apparatus lid 18, pickup wire paths 19, water guide
Passage 20 and waterflood path 21, water inlet 1 and delivery port 2 are respectively provided on catch basin 3 and wastewater trough 4, and multiplex roles pulsating pump 7 is logical
Cross and be connected respectively with the water conduction hose in waterflood path 21 and conduit pipe 20 with bacteriological filtration film water guide interface tube 6, multiplex roles are inhaled
Water pump 7 is connected by circuit with CPU microprocessor 5, the cylindrical bottle at 2/3rds away from bottleneck of culture apparatus main body 17
There is a circle projection on body wall to hold up dividing plate 15, nursery sponge 16 is laid on dividing plate 15, and culture apparatus main body 17 fills with cultivation
Put lid 18 by spiral lamination to be connected, catch basin 3 and wastewater trough 4 are connected with culture apparatus main body 17 jointly, by connecing more
Mouth pulsating pump 7 connects with water conduction hose, water conduction hose connection filtration sterilization device, ensures gnotobasis in culture apparatus, cultivation dress
Put bottom and ion concentration detector 13 is housed, humidity sensor 14 is housed in nursery sponge 16, the top of culture apparatus main body 17 is empty
Between in equipped with temp-sensitive sensor 10, oxygen/carbon dioxide concentration meter 9, bottom is provided with ion concentration detector 13, the He of catch basin 3
The bottom of wastewater trough 4 is respectively provided with PH instrument 11 and pressure sensor 12；Oxygen/carbon dioxide concentration meter 9, temp-sensitive sensor 10,
PH instrument 11, pressure sensor 12, ion concentration detector 13, humidity sensor 14 respectively by pickup wire paths 19 with
CPU microprocessor 5 is connected.Culture apparatus main body 17, the wastewater trough of catch basin 3 and 4 are made up of macromolecule PC materials.Culture apparatus
Main body 17 is 3 L cylinder, the semicircle cuboid that catch basin 3 and wastewater trough 4 are integrated with culture apparatus body 17, dividing plate 15
The hole that upper uniform aperture is 2 mm.
Culture apparatus main body 17, catch basin 3 and wastewater trough 4 form sterile interior environment after hot-air sterilization, sterile
Nutrient solution is imported in catch basin 3 by water inlet 1, and water inlet 1 is the device for more renewing nutrient solution, and catch basin 3 is storage culture
The device of liquid, described CPU microprocessor 5 are a kind of detecting system signals that can automatically monitor, regulate and control multiple condition of culture
Collect, the device of processing, the oxygen/carbon dioxide concentration meter 9 on CPU microprocessor 5, temp-sensitive sensor 10, PH instrument are set
11, pressure sensor 12, after the parameter of ion concentration detector 13 and humidity sensor 14, one kind can automatically be monitored, regulated and controled
The detecting system of the aseptic culture device of multiple condition of culture will monitor plant growth conditions in culture apparatus, oxygen/bis- constantly
Carbonoxide concentration meter 9 detects oxygen and gas concentration lwevel in culture apparatus main body 17, after CPU microprocessor 5 collects signal,
Oxygen and gas concentration lwevel ratio are processed into, is compared with institute setting parameter, when not being inconsistent with parameter, CPU microprocessor 5
Alarm will be produced, temp-sensitive sensor 10 detects the temperature in culture apparatus, when CPU microprocessor 5 collects signal and established temperature
When parameter is not inconsistent, alarm is produced.PH instrument 11 detect catch basin 3 in nutrient solution pH value, because in catch basin 3 nutrient solution for a long time not
Changing pH value will have offset, collected when CPU microprocessor 5 when signal and set PH parameters are not inconsistent and produce alarm, humidity sensor
Device 13 detects the water content of nursery sponge 16, and when reaching the mass dryness fraction parameter of setting, CPU microprocessor 5 controls multiplex roles pulsating pump
7 by will pour culture apparatus the water conduction hose that nutrient solution is injected in waterflood path 21 with bacteriological filtration film water guide interface tube 6
Water, after nursery sponge 16 suctions moisture, the humidity sensor 14 under nursery sponge 16 produces water saturation signal, the micro- places of CPU
Manage device 5 and produce and stop water filling signal, multiplex roles pulsating pump 7 stops water filling, and in the injecting process, part nutrient solution leads to culture apparatus
Cross dividing plate 15 to flow to, the nutrient solution flowed down is saved as plant by the bottom of culture apparatus main body 17, the lower part of culture apparatus main body 17
Growth nutrition and moisture are provided, when ion concentration detector 13 detects, the nutrient solution ion of the bottom of culture apparatus main body 17
When concentration is dropped to below arrange parameter, 5CPU microprocessors control multiplex roles pulsating pump 7 is connected with the spiral of water guide interface tube 8, is led to
The water conduction hose crossed in conduit pipe 20, the nutrient solution of the bottom of culture apparatus main body 17 is imported into wastewater trough 4, works as wastewater trough
When pressure sensor 12 in 4 reaches arrange parameter, CPU microprocessor 5 controls multiplex roles pulsating pump 7 and the inwall of delivery port 2
Waste liquid is exported outside wastewater trough after the connection of water conduction hose spiral.
Claims (1)
1. the aseptic culture device of multiple condition of culture can be monitored, regulated and controled automatically, it is characterised in that including：Water inlet（1）、
Delivery port（2）, catch basin（3）, wastewater trough（4）, CPU microprocessor（5）, band bacteriological filtration film water guide interface tube（6）, multiplex roles water suction
Pump（7）, water guide interface tube（8）, oxygen/carbon dioxide concentration meter（9）, temp-sensitive sensor（10）, PH instrument（11）, pressure sensing
Device（12）, ion concentration detector（13）, humidity sensor（14）, dividing plate（15）, nursery sponge（16）, culture apparatus main body
（17）, culture apparatus lid（18）, pickup wire paths（19）, conduit pipe（20）And waterflood path（21）, catch basin（3）
It is provided with water inlet（1）, wastewater trough（4）It is provided with delivery port（2）, multiplex roles pulsating pump（7）Pass through band bacteriological filtration film water guide respectively
Interface tube（6）With waterflood path（21）And conduit pipe（20）Interior water conduction hose is connected, multiplex roles pulsating pump（7）Pass through line
Road and CPU microprocessor（5）Be connected culture apparatus main body（17）Having at 2/3rds away from bottleneck on cylindrical bottle body wall
One encloses projection to hold up dividing plate（15）, nursery sponge（16）It is laid in dividing plate（15）On, culture apparatus main body（17）Filled with cultivation
Put lid（18）It is connected by spiral lamination, catch basin（3）And wastewater trough（4）Jointly with culture apparatus main body（17）It is connected,
Pass through multiplex roles pulsating pump（7）Connected with water conduction hose, water conduction hose connection filtration sterilization device, culture apparatus main body（17）Bottom
Equipped with ion concentration detector（13）, nursery sponge（16）In humidity sensor is housed（14）, culture apparatus main body（17）Top
Temp-sensitive sensor is housed in space（10）, oxygen/carbon dioxide concentration meter（9）, bottom is provided with ion concentration detector（13）, store
Tank（3）And wastewater trough（4）Bottom be respectively provided with PH instrument（11）And pressure sensor（12）, oxygen/carbon dioxide concentration meter
（9）, temp-sensitive sensor（10）, PH instrument（11）, pressure sensor（12）, ion concentration detector（13）, humidity sensor（14）
Pass through pickup wire paths respectively（19）With CPU microprocessor（5）It is connected；
Wherein, described culture apparatus main body（17）, catch basin（3）And wastewater trough（4）It is made up of macromolecule PC materials；
Described culture apparatus main body（17）For 3 L cylinder, catch basin（3）And wastewater trough（4）With culture apparatus main body
（17）The semicircle cuboid being integrated；
Described dividing plate（15）The hole that upper uniform aperture is 2 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610595893.8A CN106069776B (en) | 2016-07-27 | 2016-07-27 | The aseptic culture device of multiple condition of culture can be monitored, regulated and controled automatically |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610595893.8A CN106069776B (en) | 2016-07-27 | 2016-07-27 | The aseptic culture device of multiple condition of culture can be monitored, regulated and controled automatically |
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| Publication Number | Publication Date |
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| CN106069776A CN106069776A (en) | 2016-11-09 |
| CN106069776B true CN106069776B (en) | 2017-11-21 |
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| CN106873459A (en) * | 2017-02-23 | 2017-06-20 | 佛山市融信通企业咨询服务有限公司 | A kind of tissue culture culture medium Nutrition monitoring system |
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| CN101300958B (en) * | 2007-05-11 | 2012-01-11 | 中国科学院沈阳应用生态研究所 | Intermittent immersed plant cultivation device and control method thereof |
| CN101715722B (en) * | 2009-12-11 | 2011-06-22 | 李泳龙 | Full-automatic no-soil plant culture apparatus |
| RU103533U1 (en) * | 2010-01-15 | 2011-04-20 | ООО "Биоплант" | DEVICE FOR BIOTECHNOLOGICAL GROWING OF VEGETABLE EXPLANTS |
| KR101117115B1 (en) * | 2011-08-31 | 2012-02-22 | 주식회사 쉴드텍 | Compacted culture apparatus |
| CN104025941B (en) * | 2014-07-03 | 2016-04-20 | 金海平 | A kind of plant growing box |
| CN104798627B (en) * | 2015-04-26 | 2017-10-27 | 湖南三箭农业科技有限公司 | The automaton of plant growth environment |
| CN205962198U (en) * | 2016-07-27 | 2017-02-22 | 大连大学 | Can automatic monitoring , a plurality of culture conditions's of regulation and control aseptic cultivation device |
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Application publication date: 20161109 Assignee: Dalian Dongmatun Pinyun Agricultural Development Co.,Ltd. Assignor: DALIAN University Contract record no.: X2023210000197 Denomination of invention: A sterile cultivation device that can automatically monitor and regulate multiple cultivation conditions Granted publication date: 20171121 License type: Common License Record date: 20231127 |
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