CN207717627U - A kind of fecal sample Test paper - Google Patents
A kind of fecal sample Test paper Download PDFInfo
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- CN207717627U CN207717627U CN201721819491.8U CN201721819491U CN207717627U CN 207717627 U CN207717627 U CN 207717627U CN 201721819491 U CN201721819491 U CN 201721819491U CN 207717627 U CN207717627 U CN 207717627U
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Abstract
The utility model is related to a kind of fecal sample Test papers, include substrate, and the surface upper and lower ends of substrate are respectively equipped with blank block and black cog region, and spaced pH indicator paper blocks and lactose indicator paper block are equipped between the blank block and black cog region.Wherein, the pH indicator paper blocks and lactose indicator paper block are the filter paper blocks that surface is attached with pH detection material layers and lactose detection material layer respectively, and it is fixed on substrate by sticking double faced adhesive tape, it is respectively intended to the content of the pH value and lactose of detection infant's fecal sample, and lactose intolerance is determined whether by the testing result of lactose and pH jointly；The blank block is blank filter paper block, and is fixed on substrate by sticking double faced adhesive tape, and the influence of the colour development difference of fecal sample color generation is effectively eliminated.The structure of the fecal sample Test paper is simple, difficulty of processing and at low cost, and analysis result accuracy, accuracy are high, can quickly judge testing result, be conducive to be widely used to promote.
Description
Technical field
The utility model belongs to external diagnosis reagent case field, more particularly to a kind of to pass through the quick of dry chemical method sxemiquantitative
The content and pH value for detecting the lactose in fecal sample, to auxiliary diagnosis lactose malabsorption and the excrement sample of lactose intolerance
This Test paper.
Background technology
Lactose is one kind of disaccharides, and molecular formula is C12H22O11, it is the disaccharide in mammal milk, therefore and gain the name.
Its molecular structure is condensed and is formed by a molecule glucose and a molecule galactolipin.
Lactose is existed only in nature in the milk of mammal.Lactose content is not to the utmost in each mammal milk
It is identical.5%-7% containing lactose in human milk contains lactose 4.2% in milk, contains lactose 4.6% in Goat Milk.
Lactose is that children eat best carbohydrate, and have the sufficient lactase to reduce lactose in children's alimentary canal, can be very
Good decomposition digests and absorbs lactose.
Milk is most abundant containing lactose, and the 99.8% of contained carbohydrate is all lactose, and milk contain abundant minerals,
Calcium, phosphorus, iron, zinc, copper, manganese, molybdenum.Lactose in milk is decomposed into the small enteral of children holds digestible glucose and galactolipin.
Digesting and assimilating for galactolipin is relatively slow, but galactolipin is to promote bacterium synthesis vitamin K and vitamin B compound in children's enteron aisle
Accelerating agent.Lactose and the absorption that can promote mineral calcium, phosphorus, magnesium etc., increase blood calcium concentration, keep bone doped calcium rapider, are milk
The absorption and utilization of middle high calcium create optimal conditions, reduce the requirement of vitamin D, so milk is that human milk is good
Substitute.
After normal person eats the dairy produce containing lactose, body is helped in the metabolism of small enteral or is decomposed by lactase
Lactose.Lactase is a kind of enzyme that small intestinal cells release generates.This enzyme depends on lactose and is split into galactolipin and Portugal
Grape glycan molecule.Glucose is absorbed into blood flow at once, and is used for all cellular energies.Galactolipin cannot directly be utilized by body,
It needs to be converted further into glucose by lactase and other enzymes.
Lactose intolerance, also known as lactose digestion be bad or lactose malabsorption, refers to not generating to reduce lactose in human body
Lactase state.It is a kind of multiple inborn heredity expression in Asia.Due to cannot in the enteron aisle of patient
The enzyme to reduce lactose, and make lactose digestion, absorb, it is used in human body.Lactose can become breast in enteron aisle by bacterial decomposition
Acid to destroy the alkaline environment of enteron aisle, and makes intestinal secretion go out a large amount of alkaline digest liquid to neutralize lactic acid.So being easy hair
Raw laxativeness.
According to statistics, the whole world all has that lactose intolerance, incidence are related with race.Asian 95-100%,
Black people 60-80%, middle European 2-23%, America white man 6-22%.It is investigated according to Chinese Center for Disease Control and Prevention, China Beijing,
The incidence of the children's lactose intolerance of the ground such as Shanghai, Guangzhou 3-13 Sui and lactose malabsorption is about 80%.
Lactose intolerance can cause the symptoms such as borborygmus, abdominal distension, abdominal pain, exhaust, diarrhea, somebody to also occur that belch, dislike
Heart etc. can also cause malnutrition, while with diaper rash, vomiting, growth retardation etc..
The country has following several currently used for the method for inspection lactose intolerance.
Hydrogen (H2) breath test, it is the most common experiment of current diagnosis alactasia.This method is accurate, simple, nothing
It creates, conveniently, specificity and susceptibility are all higher than 90%, but need to be equipped with trace hydrogen analyzer, and grass-roots unit is difficult to promote；Operation
Time is long, is unfavorable for the investigation of large sample crowd；About 2% crowd does not generate H2。
Lead acetate method measures stool reduced sugar, is the current clinically used method of inspection, but operating process is complicated, and react
It needs to heat in the process.
Lactose tolerance test is traditional detection method, and different time points take blood examination to survey blood glucose level after need to taking orally lactose.
This method is easy to operate, but blood, children need to repeatedly be taken to be difficult to receive, and false positive rate is high, poor specificity.
The sugar of infant's intake is mainly lactose, and the lactose not being decomposed in enteron aisle is discharged with excrement, while containing in excrement
There is organic acid caused by lactose metabolism (acetic acid, lactic acid, propionic acid, butyric acid etc.) and pH value is made to reduce, thus the reduction in excrement
Sugared content and pH value change can embody lactose and decompose situation.
Utility model content
To solve the above-mentioned problems in the prior art, the utility model provides a kind of to be detected using dry chemical method
Lactose content in fecal sample and pH value, analysis result accuracy and accuracy are high, and operating process is simple, and can quickly judge
Testing result is conducive to the Test paper being widely used to promote.
In order to solve the above technical problems, the utility model adopts the following technical solution：
A kind of fecal sample Test paper, includes substrate, and the surface upper and lower ends of the substrate, which are respectively provided with, to disappear
The blank block and the black of test strips positive and negative for identification in automatic test for generating colour developing interference except fecal sample color
Cog region is equipped with spaced pH indicator paper blocks and lactose indicator paper block between the blank block and black cog region.
Further, the lactose indicator paper block is the filter paper block that surface is attached with lactose detection material layer, and by two-sided
Glue is fixed on substrate；The pH indicator paper blocks are the filter paper blocks that surface is attached with pH detection material layers, and pass through double faced adhesive tape
It is pasted and fixed on substrate.
Further, the blank block is blank filter paper block, and is fixed on substrate by sticking double faced adhesive tape.
Further, the substrate is made by plastic cement material.
Further, the substrate is PET porcelain white tiles.
The utility model has the beneficial effects that：
The utility model is through the above technical solutions, can pass through lactose in dry chemical method half-quantitative detection fecal sample
Content and pH value, analysis result accuracy and accuracy are high, and can quickly judge testing result, and the testing result of lactose and pH can
Combine and determine whether lactose intolerance, and Test paper structure design is simple, difficulty of processing and at low cost is conducive to push away extensively
It is wide to use.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of fecal sample Test paper embodiment described in the utility model.
Specific implementation mode
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation
Example, the present invention will be further described in detail.It should be appreciated that specific embodiment described herein is only used to explain
The utility model is not used to limit the utility model.
As shown in Figure 1, a kind of fecal sample Test paper described in the utility model embodiment, includes substrate 1, it is described
The surface upper and lower ends of substrate 1 are respectively provided with the blank block 2 for eliminating the generation colour developing interference of fecal sample color and automatic
The black cog region 5 of test strips positive and negative for identification when changing test, between being equipped between the blank block 2 and black cog region 5
Every the pH indicator paper blocks 3 and lactose indicator paper block 4 of setting.
Wherein, the substrate is made by plastic cement material, preferably PET porcelain white tiles；The blank block 2 is blank filter paper
Block, and fixed on the substrate 1 by sticking double faced adhesive tape；The pH indicator paper blocks 3 are the filter paper that surface is attached with pH detection material layers
Block, and fixed on the substrate 1 by sticking double faced adhesive tape, the pH detections material layer is by methyl red, polyvinylpyrrolidone, spits
Temperature 20 and alcohol mixing are dried, and methyl red：Polyvinylpyrrolidone：Polysorbas20 is 0.05：2：1；The lactose test paper
Block 4 is the filter paper block that surface is attached with lactose detection material layer, and on the substrate 1 by sticking double faced adhesive tape fixation, the lactose
Detection material layer is by lactase, glucose oxidase, peroxidase, potassium iodide, lemon acid buffering to, polyvinylpyrrolidine
Ketone and purified water mixing are dried, and a concentration of the 0.2% of lactase, and a concentration of the 0.4% of glucose oxidase, peroxide
A concentration of 0.2%, the pH6.6 of compound enzyme, lemon acid buffering is to for citric acid and sodium citrate salt.
The step of fecal sample Test paper described in the utility model is when being measured be specially：
(1) processing of sample：Fecal sample is diluted in accordance with the appropriate ratio using Sample dilution, make sample at
For solution；20 microlitres of sample solutions configured are pipetted respectively to be added dropwise on blank block, pH indicator paper blocks and lactose indicator paper block.
(2) hydrolysis of lactose：If there are lactose in fecal sample, then under the action of lactase, lactose hydrolysis is grape
Sugar and galactolipin；
(3) it develops the color：
1. the colour developing of lactose indicator paper block：The glucose generated is hydrolyzed under the action of glucose oxidase, it is made to generate Portugal
Grape uronic acid and hydrogen peroxide；Under the catalytic action of peroxidase, potassium iodide is aoxidized by electron acceptor of hydrogen peroxide, is made
It is from no discoloration brown；
2. the colour developing of pH indicator paper blocks：PH indicator paper blocks contain methyl red, and corresponding colour developing variation can be presented in color range,
By yellow to orange to red change procedure.
(4) colorimetric：Test paper can be under mating corresponding each concentration ranges colorimetric card, wait after the completion of developing the color, can by lactose and
The colour developing result of pH is compared with colorimetric card, judges the content and pH value of lactose in sample, completes detection.
The utility model compared with prior art, difference lies in：1, the utility model can half-quantitative detection lactose contain
The size of amount, also lactose may only be detected whether by being different from present technology；2, calculating mechanism is different, the lactose of this Test paper
Project be according to detection lactose hydrolysis after glucose content how much reckoning lactose contents number, be different from the prior art
The content of galactolipin is detected to determine whether containing lactose；3, test paper structure is different, this test paper contain blank block, pH indicator paper blocks and
Lactose indicator paper block is fixed with substrate with double faced adhesive tape, and published test paper is by lactase absorption test paper, colour developing test paper, blotting paper
Three parts test paper forms；4, this Test paper is directly measured using drop sample by the way of indicator paper block surface, is different from existing
Technology chromatographs action-reaction using blotting paper or the positive of superposition test paper；5, the detection project of test paper is different, this Test paper
Two projects of lactose and pH can be detected simultaneously, are different from the prior art and are only detected one project of lactose；6, test strips manufacture craft
Difference, the lactose Test paper block in this test strips are to dissolve lactase, glucose oxidase, peroxidase and potassium iodide
After be adsorbed on same test paper, be different from the prior art by galactose oxidase, peroxidase, color developing agent drying absorption
On same test paper, lactase is adsorbed on another filter paper or respectively by galactose oxidase, peroxidase, color developing agent
Substance is adsorbed on 3 filter paper；7, color developing agent is different, this Test paper, as color source, is different from the prior art using potassium iodide
Disclosed TMB or 4-AA and 3,5 dichloro dihydroxy benzenes sulfonic acids.
In conclusion fecal sample Test paper described in the utility model passes through dry chemical method half-quantitative detection fecal sample
The content and pH value of middle lactose, analysis result accuracy and accuracy are high, and can quickly judge testing result, the inspection of lactose and pH
Surveying result can combine and determines whether lactose intolerance, and Test paper structure design is simple, difficulty of processing and at low cost, favorably
In being widely used to promote.
The above is preferred embodiments of the present invention, it is noted that for the ordinary skill of the art
For personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these are improved and profit
Decorations are also considered as the scope of protection of the utility model.
Claims (5)
1. a kind of fecal sample Test paper, it is characterised in that：Include substrate, the surface upper and lower ends of the substrate are set respectively
It is useful for eliminating the blank block of fecal sample color generation colour developing interference and test strips is positive and negative for identification in automatic test
The black cog region in face is equipped with spaced pH indicator paper blocks and lactose indicator paper block between the blank block and black cog region.
2. fecal sample Test paper according to claim 1, it is characterised in that：The lactose indicator paper block is that surface is attached with
Lactose detects the filter paper block of material layer, and is fixed on substrate by sticking double faced adhesive tape；The pH indicator paper blocks are that surface is attached with
PH detects the filter paper block of material layer, and is fixed on substrate by sticking double faced adhesive tape.
3. fecal sample Test paper according to claim 2, it is characterised in that：The blank block is blank filter paper block, and
It is fixed on substrate by sticking double faced adhesive tape.
4. according to claims 1 or 2 or the 3 fecal sample Test papers, it is characterised in that：The substrate is by plastic cement material
It is made.
5. fecal sample Test paper according to claim 4, it is characterised in that：The substrate is PET porcelain white tiles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721819491.8U CN207717627U (en) | 2017-12-20 | 2017-12-20 | A kind of fecal sample Test paper |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721819491.8U CN207717627U (en) | 2017-12-20 | 2017-12-20 | A kind of fecal sample Test paper |
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| CN207717627U true CN207717627U (en) | 2018-08-10 |
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| CN201721819491.8U Active CN207717627U (en) | 2017-12-20 | 2017-12-20 | A kind of fecal sample Test paper |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941799A (en) * | 2017-12-20 | 2018-04-20 | 珠海科域生物工程股份有限公司 | A kind of fecal sample Test paper and preparation method thereof |
| CN109444122A (en) * | 2018-10-26 | 2019-03-08 | 南通大学 | A kind of Test paper |
| CN113341119A (en) * | 2021-05-13 | 2021-09-03 | 杭州海路医疗科技有限公司 | Method for indirectly detecting lactose intolerance |
-
2017
- 2017-12-20 CN CN201721819491.8U patent/CN207717627U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941799A (en) * | 2017-12-20 | 2018-04-20 | 珠海科域生物工程股份有限公司 | A kind of fecal sample Test paper and preparation method thereof |
| CN109444122A (en) * | 2018-10-26 | 2019-03-08 | 南通大学 | A kind of Test paper |
| CN109444122B (en) * | 2018-10-26 | 2024-04-12 | 南通大学 | Test paper |
| CN113341119A (en) * | 2021-05-13 | 2021-09-03 | 杭州海路医疗科技有限公司 | Method for indirectly detecting lactose intolerance |
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