CN206573483U - The test paper of lactose content in a kind of detection sample - Google Patents
The test paper of lactose content in a kind of detection sample Download PDFInfo
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- CN206573483U CN206573483U CN201720119078.4U CN201720119078U CN206573483U CN 206573483 U CN206573483 U CN 206573483U CN 201720119078 U CN201720119078 U CN 201720119078U CN 206573483 U CN206573483 U CN 206573483U
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Abstract
The test paper of lactose content in a kind of detection sample, chooses filter paper, does substrate vectors, with drying technology, lactase is added on filter paper and dried, TMB, horseradish peroxidase, galactose oxidase are added sequentially on another filter paper, substep is dried；Pvc plates are chosen, for the filter paper after fixed drying, lactose Test paper are finally cut into；This, which prepares test paper and can detect, whether there is lactose in sample, due to having used enzymatic reaction, it is swift in response, sensitivity is high, using test paper method, completion detection can be once loaded, it is easy to use by color change come judged result, the utility model preparation technology is simple, cost is the bottom of compared with, it is easy to promote, available for whether containing lactose in quick detection sample.
Description
Technical field：
The utility model is related to a kind of Test paper, belong to detection sample in whether the technical field containing lactose, such as
Fecal sample, be especially suitable in quick detection fecal sample whether the test paper containing lactose.
Background technology：
Lactose is mainly derived from the milk of mammal, and the milk lactose content of every kind of mammal is different
, such as, human milk contains and contains lactose 4.2% in lactose 7%, milk, and Goat Milk contains lactose 4.6%.Milk is that one kind is rich in nutrition
Drink, be breast milk best substitute for infant.Contain protein, carbohydrate, phosphatide and calcium, zinc in milk
Etc. various trace elements.Drink milk can supplement the trace element such as the nutrition such as protein and calcium, zinc；Reduce rickets, osteoporosis
The disease odds such as disease, anaemia, angiocardiopathy.
Normal person is drunk after the dairy produce containing lactose, and lactose can be brushed in small intestine positioned at small intestinal mucosa velvet hair distal end
Lactase breaks down on shape edge is a molecule glucose and a molecule galactolipin, and glucose and galactolipin are monose, Ke Yizhi
Connect and absorbed by cell, the glycolytic pathway that glucose enters continues to decompose；Galactolipin is then converted into galactolipin -1- by galactokinase
Phosphoric acid, afterwards under galactose-1-phosphate uridyl transfer enzyme effect, forms UDP- galactolipins, UDP- galactolipins are in NAD+
In the case of coenzyme, UDPG is converted to by UDP- galactolipin 4- epimerases, UPD- glucose is in UDPG
It is changed into Cori ester under pyrophosphorylation enzymatic, and then enters glycolytic pathway and continues to decompose.
Lactose intolerance refers to that the lactase secretory volume of small intestine especially Jejunal mucosa surface top of villi is reduced or living
Property is not high can not completely to be digested and decompose lactose in milk, and portion of Lactose is by colonic microflora glycolysis into lactic acid, hydrogen, methane and two
Carbonoxide.Lactic acid stimulates intestinal wall, increases enterocinesia and suffers from diarrhoea.Carbon dioxide produces flatulence in enteron aisle and increase intestines are compacted
It is dynamic, make children's performance uneasy, induction enterospasm is also possible to once in a while and intestinal colic occurs.The increase of lactic acid also drops the pH value of excrement
It is low.
According to statistics, the problem of whole world all has lactose intolerance, incidence is relevant with race, Asian 95-100%,
Black people 60-80%, middle European 2-23%, America white man 6-22%.Investigated according to CDC, China Beijing,
The children's lactose intolerance of the ground such as Shanghai, Guangzhou 3-13 Sui and lactose malabsorption incidence are about 80%.Medically recognize at present
For it is determined that the diagnosis reliable method of lactose intolerance symptom is the activity that jejunum biopsy directly determines lactase, but its is invasive
Make its hardly possible as conventional meanses.It is a kind of accurate noninvasive diagnosis method that hydrogen, which is exhaled and tests (HBT), but needs special instrument
Device, equipment, time, grass-roots unit should not promote, and also be unfavorable for the big census of population of sample size.Fehlings reagent surveys reduced sugar
It is a kind of accurately external non-invasive diagnosis, but its course of reaction needs heating, therefore diagnosis process needs the more time
With corresponding equipment.Lead acetate method is clinical at present conventional, but lead acetate has certain danger, and operating process needs
Heating, course of reaction is relative complex.This project uses the lactose in detection of ALT with dry-chemical excrement, and whole reaction is entered at normal temperatures
OK, it may not be necessary to any equipment, just can quick etiologic diagnosis.
The current dry chemical method detection published technical patent 200610056826.5 of lactose intolerance, it passes through detection
Galactolipin in urine judges the foundation of lactose intolerance, and above-mentioned patent is limited in that：1) it is detected in human urine
Galactolipin, it is necessary to increase urine purifier, the step of adding detection and cost；2) by galactose oxidase pad, peroxide
Compound enzyme pad, developer pad are superimposed after making respectively, pass through the positive chromatography action-reaction of each test paper, display after sample-adding
Mode it is not directly perceived enough.3) whether above-mentioned technology can not be to containing lactose detecting there is detection in other samples such as excrement
The limitation of sample.
Utility model content：
For defect present in prior art, the utility model provides one kind and prepared simply, easy to use, directly detects
The preparation method of the test paper of lactose and the test paper.
To achieve the above object, technical solutions of the utility model are：
The test paper of lactose content in a kind of detection sample, including：Bottom plate, lactase absorption test paper, develop the color test paper, water suction
Paper；Wherein, lactase absorption test paper, develop the color test paper, and blotting paper is attached on bottom plate fixed in the way of tail end is superimposed.
Further, lactase absorption test paper at colour developing test paper superposed positions by adhesive tape with being fixed.
Further, described lactase absorption test paper, develop the color test paper, and the length of blotting paper tail end superposition is 2-3 millimeters.
Further, described bottom plate is PVC bottom plates.
Using above-mentioned technical proposal, test paper of the present utility model, which can detect, whether there is lactose in sample.Due to using
Enzymatic reaction, is swift in response, and sensitivity is high, using test paper method, can once be loaded completion detection, be judged by color change
As a result, it is easy to use.Preparation technology is simple, and cost is the bottom of compared with, it is easy to promote, and whether contains breast available in quick detection sample
Sugar.
Brief description of the drawings：
Fig. 1 is the decomposing schematic representation of the utility model embodiment；
Fig. 2 is the combination diagram of the utility model embodiment.
Embodiment：
The utility model/utility model is further illustrated with reference to the accompanying drawings and examples.
It is a kind of test paper of lactose content in the utility model embodiment, detection sample as shown in Figure 1-2, including：Bottom plate
1, lactase absorption test paper 2, develop the color test paper 3, blotting paper 4；Wherein, lactase absorption test paper 1, colour developing examination 2 paper, blotting paper 3 with
The mode of tail end superposition is attached on bottom plate 1 fixed.The length of superposition is generally 2-3 millimeters, or other superposition length,
As long as guaranteeing the smooth lateral chromatography of specimen samples.Bottom plate 1 can be PVC bottom plates or other suitable bottom plates.Breast
Carbohydrase adsorbs test paper 2 with being fixed at the colour developing superposed positions of test paper 3 by adhesive tape 5, to ensure that specimen samples can be smoothly from lactose
Enzyme adsorbs test paper lateral chromatography to colour developing test paper.
The method of lactose content test paper, comprises the following steps in above-mentioned preparation detection sample：
1st step, lactase soln is added drop-wise on filter paper 1 until saturation state, then dried in an oven, forms lactose
Enzyme adsorbs test paper.
2nd step, adsorbs the paper slip that test paper is cut into proper width by the lactase dried, is bonded at the breast on PVC bottom plates
Carbohydrase absorption test paper position.As shown in Figure 1.
3rd step, in addition by a piece of filter paper, TMB (tetramethyl benzidine) solution is added drop-wise on the filter paper until saturation shape
State, is then dried in an oven.
4th step, the filter paper after step 3 is dried is added drop-wise to full by the solution of horseradish peroxidase and galactose oxidase
And state, dry in an oven, eventually form colour developing test paper.
5th step, the colour developing test paper 3 that step 4 is dried is cut into the paper slip of suitable width, is bonded at the colour developing on PVC bottom plates 6
Test paper position.As shown in Figure 1.
6th step, the paper slip that blotting paper is cut into suitable width, are bonded at the blotting paper position on PVC bottom plates.Such as Fig. 1 institutes
Show.
7th step, the PVC bottom plates that will be stained with lactase absorption test paper, colour developing test paper, blotting paper, are cut into cut-off knife and are adapted to width
The test paper of degree, that is, be made the test strips of detection lactose content.Wherein, between lactase absorption test paper, colour developing test paper；Colour developing examination
Respectively have between paper, blotting paper 2~3 millimeters it is overlapping, lactase absorption test paper, colour developing test paper between surface covered with adhesive tape, it is ensured that
It is connected firmly between the two.
1st step as described above, the preparation method of lactase absorption test paper is that lactase is configured into lactase soln, will
The lactase soln configured is added drop-wise on filter paper until saturation；Then saturation filter paper is put into oven for drying to try as lactase
Paper.
Contain phosphate buffer, Sodium azide, lactase, ox in the lactase absorption test paper processing solution of 1st step
Seralbumin, its component ratio is：0.02N pH7.5 PB (phosphate buffer) solution, every liter containing 1 gram Sodium azide,
The lactase of 12000 international units, 4.5g bovine serum albumin(BSA).
3rd step as described above, the 4th step, the preparation method of colour developing test paper is first to be added drop-wise to TMB solution on the filter paper directly
To saturation state, then dry in an oven.The solution of horseradish peroxidase and galactose oxidase is added drop-wise to step 3 again
Filter paper after drying is dried in an oven to saturation state, eventually forms colour developing test paper.
In the colour developing test paper processing solution of 4th step containing phosphate buffer, Sodium azide, horseradish peroxidase,
Galactose oxidase, bovine serum albumin(BSA), its component ratio is：0.02N pH7.5 PB solution, every liter containing 1 gram Sodium azide,
The horseradish peroxidase of 20000 international units, the galactose oxidase of 12000 international units, 4.5g bovine serum albumin(BSA).
As described above 3rd, 5,6 steps, lactose enzyme test peper, colour developing test paper, blotting paper are cut into suitable width respectively and pasted
In PVC board, lactose enzyme test peper and colour developing test paper have 2~3 millimeters it is overlapping, overlapping part again use adhesive tape bonding, be made lactose inspection
Test paper.
As described above, the lactose Test paper after being made, being once loaded can complete lactose resolving into galactolipin and Portugal
Grape sugar, galactolipin resolved into hydrogen peroxide again, hydrogen peroxide changes the pH value of colour developing test paper, and colour developing test paper color is become by yellowish
The whole process of au bleu.
Further, the utility model provides a kind of specific embodiment to further illustrate the utility model, processing procedure test paper：
1st, lactase soln is added drop-wise on filter paper until saturation state, then 45 DEG C of drying 1h in an oven, forms breast
Carbohydrase adsorbs test paper.Oven temperature and drying time can be adjusted according to product quantity, until product drying.
2nd, the lactase dried is adsorbed into the paper slip that test paper is cut into wide 1.6cm, the lactase being bonded on PVC bottom plates is inhaled
Attached test paper position.
3rd, a piece of filter paper is taken in addition, and 1mg/ml TMB solution is added drop-wise on the filter paper until saturation state, then dried
45 DEG C of drying 1h in case.Oven temperature and drying time can be adjusted according to product quantity, until product drying.
4th, the solution of horseradish peroxidase and galactose oxidase is added drop-wise to the filter paper after step 3 is dried to saturation shape
State, dries 1h for 45 DEG C in an oven.Oven temperature and drying time can be adjusted according to product quantity, until product drying.
The 5th, the colour developing test paper that step 4 is dried is cut into wide 2.5cm paper slip, the colour developing test paper position on PVC bottom plates is bonded at
Put.
The 6th, blotting paper be cut into wide 2cm paper slip, the blotting paper position on PVC bottom plates is bonded at.
7th, the PVC bottom plates of lactase absorption test paper, colour developing test paper, blotting paper will be stained with, wide 4.3mm examination is cut into cut-off knife
Paper slip, is finished.
Above-mentioned test paper is when in use：
1st, sample disposal and sample-adding：Excrement uses the pure water without lactose, galactolipin to be diluted by proper proportion, is configured to sample
Solution, is added dropwise 100 microlitres of sample solutions prepared to the lactase absorption test paper of lactose test strip.
2nd, reduce lactose：As there is lactose in sample, galactolipin and glucose are resolved into the presence of lactase.The mistake
Journey is completed on lactose absorption test paper 1.
3rd, develop the color：Galactolipin and sample liquid after decomposition, are attracted on colour developing test paper 3 by dry filter paper.In colour developing
Under the galactase effect of test paper, galactolipin resolves into hydrogen peroxide, and colour developing test paper color is by yellowish change au bleu.The process exists
Completed on colour developing test paper.
Compared with prior art, its difference is the utility model：1st, the utility model test paper is by detecting in excrement
It is no to judge containing lactose, prior art is different from by detecting the galactolipin in urine to judge.2nd, the material of detection is different,
Lactose in the utility model test paper detection human faecal mass sample；And the galactolipin in published technology for detection human urine；3rd, examine
The sample of survey is different, and the utility model test paper detection sample is excrement, it has been disclosed that technology for detection sample be urine.4th, before sample
Processing is different, and the utility model test paper need to only dilute stool sample (because excrement is solid)；And published technology needs to increase
Plus urine purifier；5th, test paper structure is different, and the utility model test strips are three part test paper compositions, and respectively lactase is inhaled
Attached test paper, colour developing test paper, blotting paper, liquid are acted on by lateral chromatography, are adsorbed after test paper decomposes lactose and shifted to by lactase
Develop the color test paper, and after chromogenic reaction, unnecessary liquid is absorbed by blotting paper；And published technology be by galactose oxidase pad,
Peroxidase pad, developer pad are superimposed after making respectively, pass through the positive chromatography action-reaction of each test paper after sample-adding.
6th, test paper manufacture craft is different, and galactose oxidase, peroxidase, developer are dried absorption same by the utility model test paper
On one test paper, lactase is adsorbed on another filter paper；And published technology is respectively by galactose oxidase, peroxide
Enzyme, developer substance absorption are on 3 test paper.7th, the utility model technology contains lactase breaks down milk sugar, it has been disclosed that technology
Without lactase.8th, developer is different, and the utility model test paper color development agent is TMB, and published developer is pacified for 4- amino
For than woods and 3,5 dichloro dihydroxy benzenes sulfonic acids.9th, test paper drying process of the present utility model is different.
In summary, this method, which has, prepares simple, easy to use, the features such as directly detecting lactose, with unique advantage.
Preferred embodiment example of the present utility model has shown and described in described above, as previously described, it should be understood that this practicality
It is new to be not limited to form disclosed herein, the exclusion to other embodiment is not to be taken as, and available for various other
Combination, modification and environment, and above-mentioned teaching or the skill of association area can be passed through in utility model contemplated scope described herein
Art or knowledge are modified.And the change and change that those skilled in the art are carried out do not depart from spirit and scope of the present utility model,
Then all should be in the protection domain of the utility model appended claims.
Claims (4)
1. a kind of test paper for detecting lactose content in sample, it is characterised in that：Including：Bottom plate, lactase absorption test paper, colour developing examination
Paper, blotting paper；Wherein, lactase absorption test paper, develop the color test paper, and blotting paper is attached on bottom plate solid in the way of tail end is superimposed
It is fixed.
2. a kind of test paper for detecting lactose content in sample as claimed in claim 1, it is characterised in that：Lactase adsorbs test paper
With being fixed at colour developing test paper superposed positions by adhesive tape.
3. a kind of test paper for detecting lactose content in sample as claimed in claim 2, it is characterised in that：Described lactase is inhaled
Attached test paper, develop the color test paper, and the length of blotting paper tail end superposition is 2-3 millimeters.
4. the test paper of lactose content in a kind of detection sample as described in claim 1,2 or 3, it is characterised in that：Described bottom
Plate is PVC bottom plates.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720119078.4U CN206573483U (en) | 2017-02-09 | 2017-02-09 | The test paper of lactose content in a kind of detection sample |
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| CN201720119078.4U CN206573483U (en) | 2017-02-09 | 2017-02-09 | The test paper of lactose content in a kind of detection sample |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645132A (en) * | 2017-02-09 | 2017-05-10 | 厦门信道生物技术有限公司 | Test paper for detecting lactose content of sample and preparation method of test paper |
| CN107941799A (en) * | 2017-12-20 | 2018-04-20 | 珠海科域生物工程股份有限公司 | A kind of fecal sample Test paper and preparation method thereof |
| CN110082347A (en) * | 2019-05-30 | 2019-08-02 | 中国科学院成都生物研究所 | A kind of easy glucose in urine quantitative detecting method and glucose in urine immue quantitative detection reagent box |
-
2017
- 2017-02-09 CN CN201720119078.4U patent/CN206573483U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645132A (en) * | 2017-02-09 | 2017-05-10 | 厦门信道生物技术有限公司 | Test paper for detecting lactose content of sample and preparation method of test paper |
| CN107941799A (en) * | 2017-12-20 | 2018-04-20 | 珠海科域生物工程股份有限公司 | A kind of fecal sample Test paper and preparation method thereof |
| CN110082347A (en) * | 2019-05-30 | 2019-08-02 | 中国科学院成都生物研究所 | A kind of easy glucose in urine quantitative detecting method and glucose in urine immue quantitative detection reagent box |
| CN110082347B (en) * | 2019-05-30 | 2021-12-24 | 中国科学院成都生物研究所 | Simple urine glucose quantitative detection method and urine glucose quantitative detection kit |
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