CN106483286A - A kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid - Google Patents
A kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid Download PDFInfo
- Publication number
- CN106483286A CN106483286A CN201610564286.5A CN201610564286A CN106483286A CN 106483286 A CN106483286 A CN 106483286A CN 201610564286 A CN201610564286 A CN 201610564286A CN 106483286 A CN106483286 A CN 106483286A
- Authority
- CN
- China
- Prior art keywords
- luminous substrate
- calibration object
- swine fever
- cleaning solution
- fever virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Plasma & Fusion (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention relates to a kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid, the kit includes that envelope antigen is coated plate, enzyme labelled antibody, calibration object, concentrated cleaning solution, luminous substrate A, luminous substrate B；The antigen coated microplate, using the CSFV E 2 protein of purifying as envelope antigen；Enzyme labelled antibody is CSFV monoclonal antibody of the enzyme labelled antibody by horseradish peroxidase-labeled；The calibration object, the hyper-immune serum of live vaccine immunity is diluted to concentration for 0 NU/mL, 2NU/mL, 5 NU/mL, 10 NU/mL, 30 NU/mL and 60NU/mL respectively using calibration object dilution；Contain luminol, Hydroxycoumarin, gallic acid and Tris Hcl buffer solution in luminous substrate A；Kit of the present invention has the advantages of detection time is short, and detection signal is greatly enhanced, sensitivity is high.
Description
Technical field
The present invention relates to a kind of kit, specifically a kind of antibody against swine fever virus competition chemiluminescence quantitative determination examination
Agent box.
Background technology
Swine fever also known as cholera, China are commonly called as " rinderpest ", are a kind of high degree in contact for being caused pig by CSFV
Infectious disease, it may appear that thin vessels wall denaturation, internal organs multiple hemorrhages, necrosis and infarct.The disease propagate fast, popular wide, send out
The high and death rate of sick rate is high, very harmful.The World Health Organization is classified as legal report animal epidemic, 2008 annual overhaul of China
Order《First, two, three class animal epidemic diseases record》It is classified as a class animal epidemic.China's swine fever is characterized in that with atypia pig
Pest or mild swine fever and in-pig breeding difficulty are main forms, persistent infection, subclinical infection, mixed infection and
Immuning failure phenomenon generally existing.Evaluation in the prophylactico-therapeutic measures of China based on vaccine immunity, to immune effect of vaccine at present
The main detection by hog cholera antibody level.
Therefore antibody against swine fever virus quick detection becomes control and eliminates the sick premise.Current swine fever diagnosis skill
Art, mainly has the methods such as complement fixation test (CFT), agar immunodiffusion test, virus neutralization tests.But, traditional detection method
The diagnosis process of long period, and certain experimental skill and instrument and equipment is required for, is typically only suitable for the diagnosis in laboratory.
In recent years, ELISA diagnostic techniques has been made significant headway, and has the ELISA method that many sensitiveness are high, specificity is good to transport
Use in the middle of the extensive serologic test of vaccine immunity colony, these methods are mainly indirect ELISA and blocking ELISA.This
Although a little method sensitivity are high, high specificity, there is endogenous enzyme interference, substrate is most of poisonous or lacks for carcinogen etc.
Point.A kind of safe, sensitive, efficient checkout and diagnosis method of exploitation is very necessary.
Chemiluminescence immune assay (Chemiluminescence Immunoassay, CLIA) be nearly ten years in the world
In the range of develop very fast on-radiation immunoassay, be to grow up after radiommunoassay and EIA enzyme immunoassay
A kind of supersensitive microdetermination technology.Chemiluminescence immune assay is by the high specific immune response of antigen and Hangzhoupro body
Combine with high-sensitive chemiluminescence detection technology, by the former or Hangzhoupro body in chemiluminescent substance mark Hangzhoupro, then carry out immune combination
Reaction, you can carry out qualitative, quantitative determination.The method has high sensitivity, detection range width, quick, label easy to operate
The advantages of stable, pollution-free, instrument simple economy.If two methods are effectively combined together, so that it may so that aftosa
Detection more rapidly, sensitiveer, more accurate, safer.
Content of the invention
The present invention seeks to for solve above-mentioned technical problem deficiency, provide a kind of antibody against swine fever virus competition chemiluminescence
Immue quantitative detection reagent box, its have the advantages of detection time is short, and detection signal is greatly enhanced, sensitivity is high, and achieve swine fever
Antiviral antibody quantitative determination.
The present invention by solving the technical scheme that adopts of above-mentioned technical problem is：
A kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid, including antigen coated microplate, enzyme labelled antibody, calibration
Product, concentrated cleaning solution, luminous substrate A, luminous substrate B；
The antigen coated microplate, using the CSFV E 2 protein of purifying as envelope antigen, using the carbonate buffer solution of PH9.6
CSFV E 2 protein is coated on opaque XPS；
The enzyme labelled antibody is CSFV monoclonal antibody of the enzyme labelled antibody by horseradish peroxidase-labeled；Using glutaraldehyde
Method is marked, and will be crosslinked in CSFV monoclonal antibody, glutaraldehyde and horseradish peroxidase addition container,
Gained mixture, carrying out dialysing removes excessive glutaraldehyde；In the mixture after dialysis, isopyknic glycerine is added, be placed in-
20 DEG C of preservations；
The calibration object, by live vaccine immunity hyper-immune serum using calibration object dilution be diluted to respectively concentration for 0 NU/mL,
2NU/mL, 5 NU/mL, 10 NU/mL, 30 NU/mL and 60NU/mL；
The method for building up of the dosage unit NU/mL is, according to the cell neutralization test result of serum, selects potency and is respectively entirely
Cloudy serum, 1:2、1:20、1:80、1:960、1:2560 serum, it is established that corresponding with neutralization test serum titer is linear
Relation, and set up with neutralization test serum titer be respectively full the moon serum, 1:2、1:20、1:80、1:960、1:2560 phases
Corresponding calibration object, calibration object are respectively 0,2,5,10,30,60, and set up dosage unit NU/ml；
The concentrated cleaning solution, is the PBS of the Tween-20 containing 0.05%, is diluted using distilled water during use；
Contain luminol 0.15mmol/L, Hydroxycoumarin 0.59mmol/L, gallic acid in luminous substrate A
0.35mmol/L and Tris-Hcl buffer solution 0.2mol/L, balance of water；
Contain amino acid oxidase 0.85mmol/L in luminous substrate B, Tween-20 volumetric concentration is .8%, DTPA
0.5mmol/L, vitamin C 0.12mmol/L and acetic acid-acetate buffer 0.2mol/L, balance of water；
The calibration object dilution is addition 10g bovine serum albumin(BSA) in every 1000mL PBS, adds mass concentration
For 0.5 ‰ NaN3（Three sodium nitrides）.
The method that the antibody against swine fever virus competition chemical luminescent analysis reagent kid detects antibody against swine fever virus, including
Following steps：
（1）Sample-adding takes out antigen coated microplate, and every block of plate can detect 90 parts of sample, if 6 hole of calibration object；Sample aperture adds 50 per hole
The sample of l, calibration sample wells respectively add 50 l per hole, then add 50 l enzyme conjugates immediately in each hole above；
（2）Incubation 30 ± 1 minutes in 37 DEG C of constant incubators are put in incubation；
（3）Washing plate carries out concentrated cleaning solution is diluted obtaining cleaning solution using distilled water；The liquid in each hole is abandoned into waste liquid
Cylinder, adds 28 ± 20 l of cleaning solution per hole, soaks about 30 seconds, discards cleaning solution, continuous washing 5 times；Cleaning solution the last time
After discarding, the cleaning solution remained in hole is patted dry on blotting paper；
（4）Add luminous substrate, determine 50 l luminous substrate A of addition and 50 l luminous substrate B, 18～26 DEG C of lucifuges per hole
Determine on light-emitting appearance after 5min；
（5）With calibration object luminous value as ordinate, corresponding calibration object concentration is abscissa to result judgement, draws calibration object
Four parameter and standard curves, four parameter modes are Y=(a-d)/[1+ (x/c) b]+d；（Wherein a-- absorbance higher limit, b- absorbance
Rate of rise parameter, equivalent to slope of a curve；C-- absorbance growth rate starts the concentration value for changing, equivalent to half
Number reaction density（EC50）；D-- absorbance lower limit；Y- luminous value, the actual content that x- finally gives；）By the luminous of sample
Value substitutes into the content for directly reading antibody against swine fever virus in sample in calibration curve from calibration curve, dose value >=2.5 NU/
ML, is judged to the positive；2.5 NU/mL of dose value ＜, is judged to feminine gender.
The step（4）In add 50 l luminous substrate A and 50 l luminous substrate B can be replaced per hole, luminous substrate A and
After luminous substrate B equal-volume ratio is mixed, add cleaning solution and be supplemented to 100 l.
Beneficial effect is：
1st, kit of the present invention, can detect 0～20,000,000 luminous value, and the range of linearity is big, and 30min can detect result；Traditional enzyme
Exempt from analytic approach, 0～3.5 OD value can be detected, the range of linearity is little, and 75min can detect result；Chemiluminescence achieves detection time
Short, detection signal is greatly enhanced；It is more accurately sensitive that sensitivity assays result shows which has detection.
2nd, according to the different serum titers of serum neutralization test as full the moon serum, 1:2、1:20、1:80、1:960 and 1:
2560 etc. set up corresponding calibration object concentration and unit, it is established that the linear relationship corresponding with neutralization test serum titer,
And new dosage unit NU/mL is set up, achieves antibody against swine fever virus quantitative determination in macromolecular first, it is possible to be quick
The large quantities of samples of quantitative determination, quick and precisely, with technical marked improvement.The preparation of the grand antibody of enzyme mark monoclonal antibody, it is achieved that
One-step method 30min goes out result, and serum need not dilute directly sample-adding, shorten the reaction time, simplify operating process.Using send out
Light substrate, compares traditional chromogenic substrate, after adding luminous substrate without the need for terminate can direct readings, shorten the operating time,
Carcinogenicity and harmfulness less, safer pollution-free.
Specific embodiment
A kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid, including antigen coated microplate, enzyme labelled antibody, school
Quasi- product, concentrated cleaning solution, luminous substrate A, luminous substrate B；
The antigen coated microplate, using the CSFV E 2 protein of purifying as envelope antigen, using the carbonate buffer solution of PH9.6
CSFV E 2 protein is coated on opaque XPS；
The enzyme labelled antibody is CSFV monoclonal antibody of the enzyme labelled antibody by horseradish peroxidase-labeled；Using glutaraldehyde
Method is marked, and will be crosslinked in CSFV monoclonal antibody, glutaraldehyde and horseradish peroxidase addition container,
Gained mixture, carrying out dialysing removes excessive glutaraldehyde；In the mixture after dialysis, isopyknic glycerine is added, be placed in-
20 DEG C of preservations；
The calibration object, by live vaccine immunity hyper-immune serum using calibration object dilution be diluted to respectively concentration for 0 NU/mL,
2NU/mL, 5 NU/mL, 10 NU/mL, 30 NU/mL and 60NU/mL；
The method for building up of the dosage unit NU/mL is, according to the cell neutralization test result of serum, selects potency and is respectively entirely
Cloudy serum, 1:2、1:20、1:80、1:960、1:2560 serum, it is established that corresponding with neutralization test serum titer is linear
Relation, and set up with neutralization test serum titer be respectively full the moon serum, 1:2、1:20、1:80、1:960、1:2560 phases
Corresponding calibration object, calibration object are respectively 0,2,5,10,30,60, and set up dosage unit NU/ml；Dosage unit NU/mL builds
Vertical, achieve quantitation, quick detection first, can quick large quantities of samples of quantitative determination.
The concentrated cleaning solution, is the PBS of the Tween-20 containing 0.05%, is carried out using distilled water during use
Dilution；
Contain luminol 0.15mmol/L, Hydroxycoumarin 0.59mmol/L, gallic acid in luminous substrate A
0.35mmol/L and Tris-Hcl buffer solution 0.2mol/L, balance of water；
Contain amino acid oxidase 0.85mmol/L in luminous substrate B, Tween-20 volumetric concentration is .8%, DTPA
0.5mmol/L, vitamin C 0.12mmol/L and acetic acid-acetate buffer 0.2mol/L, balance of water；
The calibration object dilution is addition 10g bovine serum albumin(BSA) in every 1000mL PBS, adds mass concentration
For 0.5 ‰ NaN3.
The method that the antibody against swine fever virus competition chemical luminescent analysis reagent kid detects antibody against swine fever virus, including
Following steps：
1st, sample-adding takes out antigen coated microplate, and every block of plate can detect 90 parts of sample, if 6 hole of calibration object.Sample aperture adds 50 l per hole
Sample, calibration sample wells adds each 50 l of S1～S6 per hole, then adds 50 l enzyme conjugates immediately in each hole above.
2nd, incubation 30 minutes in 37 DEG C of constant incubators are put in incubation（± 1 minute）.
3rd, wash plate the liquid in each hole to be abandoned into waste liquid cylinder, add 280 l of cleaning solution per hole（±20µl）, soak about 30 seconds,
Cleaning solution is discarded, continuous washing 5 times.After cleaning solution is discarded the last time, the cleaning solution remained in hole is clapped on blotting paper
Dry.
4th, add luminous substrate, determine 50 l luminous substrate A of addition and 50 l luminous substrate per hole（Also can luminous substrate
A, B equal proportion adds 100 l after mixing）, determine on light-emitting appearance after 18～26 DEG C of lucifuges 5min.
5th, with calibration object luminous value as ordinate, corresponding calibration object concentration is abscissa to result judgement, draws calibration object
Four parameter curve fit, four parameter modes be Y=(a-d)/[1+ (x/c) b]+d.The luminous value of sample is substituted into calibration curve
In, antibody against swine fever virus actual content in sample is directly read from calibration curve.
Dose value >=2.5 NU/mL, is judged to the positive；
2.5 NU/mL of dose value ＜, is judged to feminine gender.
Related experiment
First, specific assay
Operation is required to determine brickpox, pseudorabies, haemophilus parasuis, II type of pig annulus, pig breeding and is exhaled to specifications
Inhale distress syndrome virus（PRRSV）Positive serum, assay are brickpox, pseudorabies, haemophilus parasuis, pig annulus
II type, pig breeding and respiratory disorder syndrome virus（PRRSV）The concentration value of positive serum is respectively less than 2.5 NU/mL, no special
Property reaction.Data are as follows：
2nd, sensitivity assays
Sensitiveness represents with positive rate, selects 30 parts of Swine serum（Wherein 10 parts feminine genders, 10 parts of weak sun, 10 parts strong sun）,
With neutralization test as goldstandard, data are as follows：
Neutralization test potency is more than 1:10 is the positive, and potency is less than 1:10 is feminine gender；This kit dose value is more than or equal to
2.5NU/mL is the positive, and dose value is feminine gender less than 2.5NU/mL；Data are had to understand the positive symbol of this kit and neutralization test
Conjunction rate is 100%, illustrates that this kit has preferable sensitiveness.
Claims (4)
1. a kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid, including antigen coated microplate, enzyme labelled antibody, calibration
Product, concentrated cleaning solution, luminous substrate A and luminous substrate B, it is characterised in that：
The antigen coated microplate, using the CSFV E 2 protein of purifying as envelope antigen, using the carbonate buffer solution of PH9.6
CSFV E 2 protein is coated on opaque XPS；
The enzyme labelled antibody is CSFV monoclonal antibody of the enzyme labelled antibody by horseradish peroxidase-labeled；Using glutaraldehyde
Method is marked, and will be crosslinked in CSFV monoclonal antibody, glutaraldehyde and horseradish peroxidase addition container,
Gained mixture, carrying out dialysing removes excessive glutaraldehyde；In the mixture after dialysis, isopyknic glycerine is added, be placed in-
20 DEG C of preservations；
The calibration object, by live vaccine immunity hyper-immune serum using calibration object dilution be diluted to respectively concentration for 0 NU/mL,
2NU/mL, 5 NU/mL, 10 NU/mL, 30 NU/mL and 60NU/mL；
The method for building up of the dosage unit NU/mL is, according to the cell neutralization test result of serum, selects potency and is respectively entirely
Cloudy serum, 1:2、1:20、1:80、1:960、1:2560 serum, it is established that corresponding with neutralization test serum titer is linear
Relation, and set up with neutralization test serum titer be respectively full the moon serum, 1:2、1:20、1:80、1:960、1:2560 phases
Corresponding calibration object, calibration object are respectively 0,2,5,10,30,60, and set up dosage unit NU/ml；
The concentrated cleaning solution, is the PBS of the Tween-20 containing 0.05%, is diluted using distilled water during use；
Contain luminol 0.15mmol/L, Hydroxycoumarin 0.59mmol/L, gallic acid in luminous substrate A
0.35mmol/L and Tris-Hcl buffer solution 0.2mol/L, balance of water；
Contain amino acid oxidase 0.85mmol/L in luminous substrate B, Tween-20 volumetric concentration is .8%, DTPA
0.5mmol/L, vitamin C 0.12mmol/L and acetic acid-acetate buffer 0.2mol/L, balance of water.
2. antibody against swine fever virus as claimed in claim 1 compete chemical luminescent analysis reagent kid, it is characterised in that：The school
Quasi- product dilution is addition 10g bovine serum albumin(BSA) in every 1000mL PBS, and it is 0.5 ‰ to add mass concentration
NaN3.
3. antibody against swine fever virus competition chemical luminescent analysis reagent kid as claimed in claim 1 detects antibody against swine fever virus
Method, it is characterised in that：Comprise the following steps：
（1）Sample-adding takes out antigen coated microplate, and every block of plate can detect 90 parts of sample, if 6 hole of calibration object；Sample aperture adds 50 per hole
The sample of l, calibration sample wells respectively add 50 l per hole, then add 50 l enzyme conjugates immediately in each hole above；
（2）Incubation 30 ± 1 minutes in 37 DEG C of constant incubators are put in incubation；
（3）Washing plate carries out concentrated cleaning solution is diluted obtaining cleaning solution using distilled water；The liquid in each hole is abandoned into waste liquid
Cylinder, adds 28 ± 20 l of cleaning solution per hole, soaks about 30 seconds, discards cleaning solution, continuous washing 5 times；Cleaning solution the last time
After discarding, the cleaning solution remained in hole is patted dry on blotting paper；
（4）Add luminous substrate, determine 50 l luminous substrate A of addition and 50 l luminous substrate B, 18～26 DEG C of lucifuges per hole
Determine on light-emitting appearance after 5min；
（5）With calibration object luminous value as ordinate, corresponding calibration object concentration is abscissa to result judgement, draws calibration object
Four parameter and standard curves, four parameter modes are Y=(a-d)/[1+ (x/c) b]+d；The luminous value of sample is substituted in calibration curve,
From on calibration curve directly read sample in antibody against swine fever virus content, dose value >=2.5 NU/mL, be judged to the positive；Agent
2.5 NU/mL of value ＜, is judged to feminine gender.
4. antibody against swine fever virus competition chemical luminescent analysis reagent kid as claimed in claim 1 detects antibody against swine fever virus
Method, it is characterised in that：The step（4）In add 50 l luminous substrate A and 50 l luminous substrate B can be replaced per hole, send out
After light substrate A and luminous substrate B equal-volume ratio are mixed, add cleaning solution and be supplemented to 100 l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610564286.5A CN106483286A (en) | 2016-07-18 | 2016-07-18 | A kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610564286.5A CN106483286A (en) | 2016-07-18 | 2016-07-18 | A kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106483286A true CN106483286A (en) | 2017-03-08 |
Family
ID=58273122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610564286.5A Pending CN106483286A (en) | 2016-07-18 | 2016-07-18 | A kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106483286A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108318682A (en) * | 2018-03-02 | 2018-07-24 | 中国农业科学院兰州兽医研究所 | Antibody against swine fever virus indirect chemiluminescence qualitative detection kit and its detection method |
| CN111323585A (en) * | 2020-02-27 | 2020-06-23 | 北京健平金星生物科技有限公司 | Preparation method of nuclear matrix protein 22 enzyme-linked immunoassay kit and kit |
| CN114675024A (en) * | 2022-03-03 | 2022-06-28 | 中国农业科学院兰州兽医研究所 | Porcine Seneca virus A neutralizing antibody, competitive ELISA detection kit and detection method |
| CN116068175A (en) * | 2022-09-07 | 2023-05-05 | 中国农业科学院兰州兽医研究所 | Swine fever virus tubular chemiluminescent antibody detection kit based on E2 protein dimer and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102707055A (en) * | 2012-06-11 | 2012-10-03 | 郑州安图绿科生物工程有限公司 | Kit for joint or single detection of autoimmune liver disease related antibody and detection method of kit |
-
2016
- 2016-07-18 CN CN201610564286.5A patent/CN106483286A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102707055A (en) * | 2012-06-11 | 2012-10-03 | 郑州安图绿科生物工程有限公司 | Kit for joint or single detection of autoimmune liver disease related antibody and detection method of kit |
Non-Patent Citations (3)
| Title |
|---|
| 张敏娜等: "化学发光法检测抗HEV IgG试剂盒的研制和评价", 《细胞与分子免疫学杂志》 * |
| 梁冰冰等: "猪瘟病毒中和抗体竞争抑制ELISA 检测方法的建立", 《中国兽医科学》 * |
| 王茹: "《运动训练功能评定测试方法》", 31 October 2012, 复旦大学出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108318682A (en) * | 2018-03-02 | 2018-07-24 | 中国农业科学院兰州兽医研究所 | Antibody against swine fever virus indirect chemiluminescence qualitative detection kit and its detection method |
| CN111323585A (en) * | 2020-02-27 | 2020-06-23 | 北京健平金星生物科技有限公司 | Preparation method of nuclear matrix protein 22 enzyme-linked immunoassay kit and kit |
| CN114675024A (en) * | 2022-03-03 | 2022-06-28 | 中国农业科学院兰州兽医研究所 | Porcine Seneca virus A neutralizing antibody, competitive ELISA detection kit and detection method |
| CN114675024B (en) * | 2022-03-03 | 2023-08-15 | 中国农业科学院兰州兽医研究所 | Pig type-A sai virus neutralizing antibody, competitive ELISA detection kit and detection method |
| CN116068175A (en) * | 2022-09-07 | 2023-05-05 | 中国农业科学院兰州兽医研究所 | Swine fever virus tubular chemiluminescent antibody detection kit based on E2 protein dimer and application thereof |
| CN116068175B (en) * | 2022-09-07 | 2024-06-07 | 中国农业科学院兰州兽医研究所 | Swine fever virus tubular chemiluminescent antibody detection kit based on E2 protein dimer and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101196518B (en) | Hepatitis virus type C immune body chemiluminescence method diagnostic reagent kit and its producing method | |
| CN106483286A (en) | A kind of antibody against swine fever virus compete chemical luminescent analysis reagent kid | |
| CN105527442B (en) | A kind of hog cholera antibody detecting system and preparation method thereof | |
| CN111929433B (en) | African swine fever virus antibody ELISA detection kit and preparation method thereof | |
| CN102175867A (en) | Enzyme-linked immunoassay kit for detecting rabies virus antibody | |
| CN103344770B (en) | Brucella abortus indirect ELISA testing kit | |
| CN106226519A (en) | A kind of O type antibodies against foot-and-mouth disease virus chemiluminescence detection kit | |
| CN101377504A (en) | Chemiluminescence immune analysis determination reagent kit for detecting Toxoplasma Gondi IgM antibody | |
| EP4113121A1 (en) | Antigen for 2019 novel coronavirus and detection use thereof | |
| CN102368071B (en) | Chemiluminescent immunoassay kit for detecting mycoplasma pneumoniae IgM antibody | |
| CN105675866A (en) | Solid-phase blocked ELISA (Enzyme Linked Immune Sorbent Assay) kit for detecting O-type FMDV (Foot and Mouth Disease Virus) antibody | |
| CN111596064B (en) | Rapid test card for simultaneously detecting PEDV and TGEV and preparation and use methods thereof | |
| CN105759033A (en) | Identification detecting method for Cox A16 and EV71 viral antigens | |
| CN101592660A (en) | Brucellosis indirect enzyme-linked immunosorbent assay milk humoral antibody detection kit | |
| CN104280551A (en) | Duck tembusu virus E-ELISA (E-enzyme-linked immuno sorbent assay) detection kit and preparation method thereof | |
| Hubbard et al. | Comparison of two automated immunoassays for the detection of SARS-CoV-2 nucleocapsid antibodies | |
| CN101655495B (en) | Detection method of EV71 virus antigen | |
| CN117229367A (en) | African swine fever virus epitope polypeptide and ELISA antibody detection kit | |
| CN102368068B (en) | Kit for detecting chlamydia pneumoniae IgM antibody | |
| CN114994306B (en) | Application of protein PKNOX1 in preparation of reagent for diagnosing alcoholic cardiomyopathy and diagnostic kit | |
| CN102809653A (en) | Preparation and application of ELISA (Enzyme-Linked Immunosorbent Assay) kit for detecting novel bunyavirus antigen | |
| WO2021217506A1 (en) | Application of specific iga and igm in early evaluation of risk of suffering from covid-19 | |
| Tabata et al. | Some species differences in the false prolongation of prothrombin times and activated partial thromboplastin times in toxicology | |
| CN102221619A (en) | ELISA (Enzyme-Linked Immuno Sorbent Assay) detection method and kit of cryptosporidium virus capsid protein antibody | |
| CN105445458A (en) | ELISA (Enzyme-linked Immuno Sorbent Assay) detection kit for identifying duck Tembusu virus infected animals and inactivated vaccine animals |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Xu Lu Inventor after: Li Cui Inventor after: Wang Qin Inventor after: Li Xiumei Inventor after: Zhao Qizu Inventor after: Ren Xuejian Inventor after: Yang Jinsong Inventor after: Zhang Qianyi Inventor after: Zhu Yuanyuan Inventor after: Zou Xingqi Inventor before: Li Xiumei Inventor before: Wang Qin Inventor before: Liu Yubao Inventor before: Xu Lu Inventor before: Geng Yujing Inventor before: Ren Xuejian Inventor before: Li Ying |
|
| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20170302 Address after: 100081 Beijing, Zhongguancun, South Street, No. 8, No. Applicant after: China Veterinery Drug Inspection Office Applicant after: LUOYANG LAIPSON INFORMATION TECHNOLOGY CO., LTD. Address before: 471000 Luoyang, Luolong, science and Technology Park, west of Peony Road, No. N3, No. Applicant before: LUOYANG LAIPSON INFORMATION TECHNOLOGY CO., LTD. |
|
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170308 |