CN207659420U - Cleavable unicellular transfer chip and unicellular capture transfer system - Google Patents
Cleavable unicellular transfer chip and unicellular capture transfer system Download PDFInfo
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- CN207659420U CN207659420U CN201721473021.0U CN201721473021U CN207659420U CN 207659420 U CN207659420 U CN 207659420U CN 201721473021 U CN201721473021 U CN 201721473021U CN 207659420 U CN207659420 U CN 207659420U
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Abstract
The utility model discloses a kind of cleavable unicellular transfer chip and unicellular capture transfer systems.Unicellular transfer chip includes liquid stream layer, it includes one or more carrying channel, each carrying channel is connected to by carrying channel entrance and carrying channel outlet with chip exterior, unicellular pond is additionally provided at least one carrying channel, unicellular pond is unicellular in the current-carrying for flowing through the carrying channel to capture；Micro-valve at least is used to make regional area and remaining regional connectivity of the liquid stream layer of the liquid stream layer comprising any one unicellular pond or is isolated；And cut mark, it is set to around the regional area of the liquid stream layer comprising any one unicellular pond, and be at least used to identify the unicellular separating element.Unicellular capture transfer system provided by the utility model can efficiently quickly finish single celled capture and transfer, and without carrying out secondary transferring, it is possible to prevente effectively from pollution in unicellular transfer process and the problem of be difficult to find confirmation.
Description
Technical field
More particularly to a kind of cleavable unicellular transfer chip of the utility model and unicellular capture transfer system, belong to
Microfluidic art.
Background technology
With the continuous development of biotechnology research means, the research level of biology is just from cell population to monolayer
Face in-depth is expanded.Each cell is unique on space-time, although they may be from same ancestors, is different
Space-time environment determines the special genetic expression of each, and to produce different biological characters, this is for evolution, drug resistance
Property, the researchs such as gene expression there is important value.However the analysis method based on cell population often covers difference in group
The difference that iuntercellular occurs, ignores these a small amount of but important information.Therefore there is an urgent need to research and develop be based on it is single celled culture and
Analysis method understands heredity and the metabolic mechanism of cell for studying the otherness between different cell individuals.
It is the first step for carrying out unicellular culture and analysis that individual cells are obtained from a large amount of cells.It is traditional unicellular to obtain
Method is taken frequently with the method for Macrodilution or micromanipulation is carried out to cell mass to carry out.Whole operation step complexity is numerous
Trivial, unicellular acquisition efficiency is low.Microflow control technique is that last century the nineties grow up in analytical chemistry field, it is with micro-
Piping network microstructure features, by micro-processing technology by functions such as microchannel, Micropump, micro-valve, micro- liquid storage device, micro- detecting elements
Component is integrated in as integrated circuit on chip material.The microflow control technique effect very high at the unicellular aspect of separating trap
Rate can be a large amount of unicellular in short period capture by making the structures such as micropore, Microtraps, microelectrode on chip, slender
Born of the same parents have had multiple successful exemplary applications in terms of capturing culture studies.For example, the documents such as CN105441309A disclose list
Cell capture chip can be captured from liquid stream containing cell by the chip and obtain multiple unicellular, but it is will be single
During cell produces, exported in meta system in chip interface and the easy adherent cell of tube wall, and by the unicellular of capture
After be difficult to find confirmation.
Utility model content
The main purpose of the utility model is to provide a kind of cleavable unicellular transfer chips and unicellular capture to turn
Shifting system, with overcome the deficiencies in the prior art.
For the aforementioned purpose of utility model of realization, the technical solution adopted in the utility model includes：
The utility model embodiment provides a kind of cleavable unicellular transfer chip, including：
Liquid stream layer comprising one or more carrying channel, each carrying channel are logical by carrying channel entrance and current-carrying
Road outlet is connected to chip exterior, and unicellular pond is additionally provided on wherein at least one carrying channel, the unicellular pond
It is unicellular in the current-carrying for flowing through the carrying channel to capture；
Micro-valve at least is used to make the regional area of the liquid stream layer comprising any one unicellular pond and the liquid stream layer
Remaining regional connectivity or isolation；And
Cut mark is set to around the regional area of the liquid stream layer comprising any one unicellular pond, and extremely
Few each unicellular separating element for identifying on the unicellular transfer chip.
Further, the cleavable unicellular transfer chip further includes the elasticity being arranged on the carrying channel
Film, the micro-valve include one or more pneumatic channel being arranged in elastic membrane, are set when inputting to have into the pneumatic channel
When the fluid of constant-pressure, the pneumatic channel can drive the regional area of the elastic membrane to squeeze carrying channel, in institute
The selected location stated in carrying channel blocks current-carrying.
Further, a unicellular pond is provided only on each carrying channel.
Further, Microtraps is distributed in the unicellular pond, the Microtraps flows through the unicellular pond to retain
Current-carrying in individual cells, the Microtraps includes spaced a plurality of microtrabeculaes.
Further, the cut mark includes transverse cuts label and longitudinally cutting label, the longitudinally cutting mark
Score and be distributed between two neighboring unicellular pond, transverse cuts label includes transverse cut, the transverse cut and
Carrying channel intersects.
Further, the unicellular separating element isolated by cutting include a unicellular pond, and with it is described unicellular
Current-carrying entrance, the current-carrying in pond are exported by plugging mechanism sealed set, and the plugging mechanism uses sealing compound.
Further, the cleavable unicellular transfer chip further includes basal layer, and the liquid stream layer is with strippable
Mode is arranged on the basal layer.
The utility model embodiment additionally provides a kind of unicellular capture transfer system, including described cleavable slender
Dysuria with lower abdominal colic moves chip and unicellular capture chip, the carrying channel entrance and unicellular capture chip of the unicellular transfer chip
Fluid course outlet.
Further, it is described it is unicellular transfer chip in each carrying channel carrying channel entrance with it is described slender
Born of the same parents capture the fluid course outlet of the corresponding fluid course in chip, all carrying channels in the unicellular transfer chip
Carrying channel outlet to it is described it is unicellular transfer chip on a corresponding carrier fluid outlet.
Further, the unicellular transfer chip is arranged with unicellular capture integrated chip.
Compared with prior art, unicellular capture transfer system provided by the utility model is simple in structure, only in application
It needs to be injected separately into each carrying channel comprising single celled current-carrying, and it is made to be flowed along carrying channel, then led to by each current-carrying
On road unicellular pond capture, later by will include the unicellular transfer chip for capturing single celled unicellular pond part
Region is partitioned into from chip, then is packaged, you can it is conveniently accomplished unicellular capture and transfer, it can be to avoid single in capture
Cell contamination in the transfer process of cell and the problem of being difficult to find confirmation.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of unicellular transfer chip in one exemplary embodiments of the utility model；
Fig. 2 is a kind of partial structurtes enlarged drawing of unicellular transfer chip in one exemplary embodiments of the utility model；.
Fig. 3 is a kind of structural schematic diagram in unicellular pond in one exemplary embodiments of the utility model；
Fig. 4 is a kind of structural schematic diagram of unicellular separating element in one exemplary embodiments of the utility model；
Fig. 5 is a kind of structural schematic diagram of unicellular capture transfer system in one exemplary embodiments of the utility model；
Fig. 6 is a kind of unicellular flow diagram for capturing, encapsulating in one exemplary embodiments of the utility model.
Specific implementation mode
In view of deficiency in the prior art, inventor is able to propose that this practicality is new through studying for a long period of time and largely putting into practice
The technical solution of type.The technical solution, its implementation process and principle etc. will be further explained as follows.
The utility model embodiment provides a kind of unicellular capture transfer method comprising：
The carrying channel entrance of cleavable unicellular transfer chip and the fluid course of unicellular capture chip are exported
Connection；
Unicellular by unicellular capture chip capture is transferred in the corresponding unicellular pond of unicellular transfer chip；
Micro-valve closing that is on chip and being loaded with selected single celled selected unicellular pond cooperation is shifted by unicellular,
Remaining zone isolation for making regional area and the liquid stream layer comprising the liquid stream layer for selecting unicellular pond, it is slender to form one
Born of the same parents' separating element；
The unicellular separating element is partitioned into from the unicellular transfer chip, and at least will be with the selected list
The current-carrying entrance of cell pool, current-carrying outlet block, and the unicellular pond is made to be isolated from the outside.
Further, the unicellular capture transfer method specifically includes：Carrier fluid is injected into unicellular capture chip,
To which the unicellular of unicellular capture chip capture to be transferred in the corresponding carrying channel of unicellular transfer chip, and then make list
Cell is captured by the corresponding unicellular pond in corresponding carrying channel.
Further, the unicellular capture transfer method may also include：By the unicellular separating element from institute
State after being cut into unicellular transfer chip, at least use the sealing compounds such as paraffin and/or epoxy glue will with it is described selected slender
The current-carrying entrance in born of the same parents pond, current-carrying outlet block, to make the unicellular pond be isolated from the outside.
Further, the unicellular capture transfer method may also include：It is single that the unicellular separation will be packaged in
In Single cell release to set environment in member.
Preferably, the unicellular capture transfer method may include：By the way that the unicellular separating element is disassembled, and
Unicellular pond therein is set to be exposed in set environment, unicellular to make wherein to encapsulate is released.
The utility model embodiment additionally provides a kind of unicellular capture transfer system comprising cleavable slender dysuria with lower abdominal colic
Move chip, the fluid course outlet of the carrying channel entrance and unicellular capture chip of the unicellular transfer chip.
In some embodiments, unicellular transfer chip above-mentioned may include：
Liquid stream layer comprising one or more carrying channel, each carrying channel are logical by carrying channel entrance and current-carrying
Road outlet is connected to chip exterior, and unicellular pond is additionally provided on wherein at least one carrying channel, the unicellular pond
It is unicellular in the current-carrying for flowing through the carrying channel to capture；
Micro-valve at least is used to make the regional area of the liquid stream layer comprising any one unicellular pond and the liquid stream layer
Remaining regional connectivity or isolation；And
Cut mark is set to around the regional area of the liquid stream layer comprising any one unicellular pond, and extremely
Less for identifying the unicellular separating element.
In some embodiments, the unicellular transfer chip further includes the elasticity being arranged on the carrying channel
Film, the micro-valve include one or more pneumatic channel being arranged in elastic membrane, are set when inputting to have into the pneumatic channel
When the fluid of constant-pressure, the pneumatic channel can drive the regional area of the elastic membrane to squeeze carrying channel, in institute
The selected location stated in carrying channel blocks current-carrying.
Further, Microtraps is distributed in the unicellular pond, the Microtraps flows through the unicellular pond to retain
Individual cells in current-carrying, the Microtraps include spaced a plurality of microtrabeculaes.
Further, wherein between the microtrabeculae neighbouring with unicellular pond inner wall and unicellular pond inner wall and/or adjacent two
Be formed between a microtrabeculae can block the individual cells in current-carrying by gap.These gaps are preferably micron-sized.
Further, the shape of the microtrabeculae is selected from the rule such as cylinder, rectangle, halfpace, cone or irregular shape
Any one kind in shape or two or more combinations, but not limited to this.
Further, unicellular pond there are one being respectively provided on each carrying channel.
Further, it is described it is unicellular transfer chip each carrying channel carrying channel entrance with it is unicellular
Capture the corresponding fluid course outlet on chip.
In some embodiments, unicellular transfer chip above-mentioned can also include basal layer, and wherein liquid stream layer can be with
It is formed on the base layer, and liquid stream layer can be removed with basal layer.It when in use, can be by the way that current carrying layer and basal layer be removed
The mode of dismantling makes unicellular pond therein be exposed, and unicellular to make wherein to encapsulate is released.
Further, the cut mark includes transverse cuts label and longitudinally cutting label, the longitudinally cutting label
It is distributed between two neighboring unicellular pond, the transverse cuts label includes transverse cut, the transverse cut and load
Circulation road intersects.
Further, the unicellular transfer chip can be arranged with unicellular capture integrated chip.
Further, in some embodiments of the utility model, the unicellular capture transfer system of one kind can have
There is integrated chip structure, and includes more than one unicellular capture chip module and more than one cleavable unicellular transfer core
Piece module, wherein each unicellular capture chip module can coordinate with one or more unicellular transfer chip modules, or
Each unicellular transfer chip module can coordinate with one or more unicellular capture chip modules.
Wherein, the foundation structure phase of the foundation structure of the unicellular capture chip module and aforementioned unicellular capture chip
Together.
Wherein, the foundation structure phase of the foundation structure of the unicellular transfer chip module and aforementioned unicellular transfer chip
Together.
Wherein, the unicellular capture chip may be used as CN105441309A, CN105441308A,
The unicellular capture chip of the records such as CN105441307A.
Further, the unicellular capture chip of the utility model, unicellular capture chip module, unicellular transfer core
The manufactures such as MEMS technology may be used in piece, unicellular transfer chip module etc., and may be used inorganic or organic material.
In order to make unicellular transfer chip be easy to be cut, it is preferred to use the easy cutting such as PDMS (dimethyl silicone polymer),
The agent structure of the unicellular transfer chip is made in easily-disassembled material.
It as follows will in conjunction with the embodiments and attached drawing is further explained the technical solution, its implementation process and principle etc.
It is bright.
In one embodiment of the utility model, a kind of unicellular slender dysuria with lower abdominal colic for capturing transfer system and including cleavable
Chip is moved, is please referred to Fig.1 shown in-Fig. 2, the unicellular transfer chip includes：
Liquid stream layer comprising several carrying channels 1, each carrying channel pass through carrying channel entrance 12 and carrying channel
Outlet 13 is connected to chip exterior, and is also set up on each carrying channel 1 there are one unicellular pond 11, and the unicellular pond is used
Flowed through with capture unicellular in the current-carrying of the carrying channel, the inside has and only there are one cell in each carrying channel, with
Liquid stream directed flow in channel；
Micro-valve is used to make regional area and the liquid stream layer of the liquid stream layer comprising any one unicellular pond remaining
Regional connectivity or isolation；And
Cut mark is set to around the regional area of the liquid stream layer comprising any one unicellular pond, and extremely
Less for identifying the unicellular separating element.
Further, the carrying channel entrance of each carrying channel in the unicellular transfer chip is caught with unicellular
Obtain the corresponding fluid course outlet (being wherein not shown) of chip；And each carrying channel carrying channel outlet can with it is described
A carrier fluid outlet on unicellular transfer chip.
Further, the unicellular transfer chip further include the elastic membrane that is arranged on the carrying channel (in figure not
Show), the micro-valve includes several pneumatic channels 2 being arranged in elastic membrane, is set when inputting to have into the pneumatic channel
When the fluid of constant-pressure, the pneumatic channel can drive the regional area of the elastic membrane to squeeze carrying channel, in institute
The selected location stated in carrying channel blocks current-carrying.
Further, the cut mark includes transverse cuts label and longitudinally cutting label 4, the longitudinally cutting label
It is distributed between two neighboring unicellular pond, the transverse cuts label includes transverse cut 3, the transverse cut and load
Circulation road intersects.
Further, it please refers to shown in Fig. 3, is distributed with Microtraps 110 in the unicellular pond 11, the Microtraps is retaining
The individual cells in the current-carrying in the unicellular pond are flowed through, the Microtraps includes spaced a plurality of microtrabeculaes.
Further, wherein between the microtrabeculae neighbouring with unicellular pond inner wall and unicellular pond inner wall and/or adjacent two
Be formed between a microtrabeculae can block the individual cells in current-carrying by gap, these gaps are preferably micron-sized.
Further, the shape of the microtrabeculae is selected from the rule such as cylinder, rectangle, halfpace, cone or irregular shape
Any one kind in shape or two or more combinations, but not limited to this.
May include by the method that aforementioned unicellular capture transfer system carries out unicellular capture transfer：
Each carrying channel entrance of the unicellular transfer chip is gone out to the corresponding fluid course of unicellular capture chip
Mouth connection；
Carrier fluid is injected into the fluid course of unicellular capture chip, make wherein to capture unicellular is transferred to list with carrier fluid
Cell shifts in the corresponding carrying channel of chip, and then the unicellular corresponding unicellular pond by corresponding carrying channel is made to catch
It obtains；
Micro-valve closing that is on chip and being loaded with selected single celled selected unicellular pond cooperation is shifted by unicellular,
Remaining zone isolation for making regional area and the liquid stream layer comprising the liquid stream layer for selecting unicellular pond, it is slender to form one
Born of the same parents' separating element；
The unicellular separating element is partitioned into from the unicellular transfer chip, and at least will be with the selected list
The current-carrying entrance of cell pool, current-carrying outlet block, and the unicellular pond is made to be isolated from the outside.
Wherein, it please refers to shown in Fig. 4, is cut into from the unicellular transfer chip by the unicellular separating element
Later, the sealing compounds such as paraffin and/or epoxy glue 6, which may be used, to be exported with the current-carrying entrance for selecting unicellular pond, current-carrying
It blocks, to make the unicellular pond be isolated from the outside.
Further, can also be by the way that the unicellular separating element be disassembled, and unicellular pond therein is made to be exposed to
Set environment (such as be suitble to the culture single celled environment or test institute is carried out to the unicellular separating element shape of single celled property
The environment needed) in, unicellular to make wherein to encapsulate is released.
In some preferred embodiments, unicellular transfer chip above-mentioned can be set with unicellular capture integrated chip
It sets.For example, please referring to shown in Fig. 5, a unicellular capture chip and multiple unicellular transfer integrated chip settings can be existed
In one integrated chip, to form an integrated unicellular capture transfer system.That is, the unicellular capture chip and list
Cell shifts chip as the function module of integrated chip.Wherein, the load of each carrying channel in unicellular transfer chip
Flow channel inlet to it is unicellular capture chip corresponding fluid course outlet, and the carrying channel of each carrying channel export
Can with it is described it is unicellular transfer chip on a carrier fluid outlet.
When the application unicellular capture transfer system carries out single celled capture and shifts, please refer to shown in Fig. 6, it can be with
The unicellular capture chip that cell suspension comprising multiple cells is inputted to the integrated chip carries out single celled capture, later will
The unicellular of capture is transferred to unicellular transfer chip, and single celled encapsulation, transfer are carried out further in accordance with manner as described above.This
A process can quickly complete in an integrated chip, efficient quick, and small to the loss of sample.Then, it is needing to turning
That moves is unicellular when being cultivated, being tested, and can disassemble the unicellular separating element being partitioned into and (wherein schematically illustrate
Liquid stream layer and basal layer, and do not identify pneumatic channel etc.), so that unicellular pond therein is exposed to outer, and then by such as rushing
It the modes such as washes, unicellular can be transferred to therein in required culture or test environment.
By the unicellular capture transfer method and system of the utility model, single celled catch can be efficiently quickly finished
It obtains and shifts, and without carrying out secondary transferring, it is possible to prevente effectively from the cell contamination in the transfer process after capture is unicellular
The problem of with being difficult to find confirmation.
It should be appreciated that above-described embodiment is only the technical concepts and features for illustrating the utility model, it is ripe its object is to allow
The personage for knowing technique can understand the content of the utility model and implement according to this, can not limit the utility model with this
Protection domain.It is all according to equivalent change or modification made by the spirit of the present invention essence, should all cover in the utility model
Within protection domain.
Claims (10)
1. a kind of cleavable unicellular transfer chip, it is characterised in that including：
Liquid stream layer comprising one or more carrying channel, each carrying channel are gone out by carrying channel entrance and carrying channel
Mouthful be connected to chip exterior, and be additionally provided with unicellular pond on wherein at least one carrying channel, the unicellular pond to
Capture flows through unicellular in the current-carrying of the carrying channel；
Micro-valve, at least remaining of the regional area for making the liquid stream layer comprising any one unicellular pond and the liquid stream layer
Regional connectivity or isolation；And
Cut mark is set to around the regional area of the liquid stream layer comprising any one unicellular pond, and at least uses
In identify it is described it is unicellular transfer chip on each unicellular separating element.
2. cleavable unicellular transfer chip according to claim 1, it is characterised in that further include being arranged in the load
Elastic membrane on circulation road, the micro-valve include one or more pneumatic channel being arranged in elastic membrane, are pneumatically led to when to described
When input has the fluid of setting pressure in road, the pneumatic channel can drive the regional area of the elastic membrane to squeeze current-carrying
Channel, to which the selected location in the carrying channel blocks current-carrying.
3. cleavable unicellular transfer chip according to claim 1, it is characterised in that：It is only set on each carrying channel
It is equipped with a unicellular pond.
4. cleavable unicellular transfer chip according to claim 1 or 3, it is characterised in that：In the unicellular pond
Microtraps is distributed with, the Microtraps is to retain the individual cells in the current-carrying for flowing through the unicellular pond, between the Microtraps includes
Every a plurality of microtrabeculaes of setting.
5. cleavable unicellular transfer chip according to claim 1, it is characterised in that：The cut mark includes cross
To cut mark and longitudinally cutting label, the longitudinally cutting indicia distribution is between two neighboring unicellular pond, the transverse direction
Cut mark includes transverse cut, and the transverse cut is intersected with carrying channel.
6. cleavable unicellular transfer chip according to claim 1, it is characterised in that：It is isolated by cutting slender
Born of the same parents' separating element includes a unicellular pond, and close by plugging mechanism with the current-carrying entrance in the unicellular pond, current-carrying outlet
Envelope setting, the plugging mechanism use sealing compound.
7. cleavable unicellular transfer chip according to claim 1, it is characterised in that further include basal layer, the liquid
Fluid layer is arranged in a releasable manner on the basal layer.
8. a kind of unicellular capture transfer system, it is characterised in that including cleavable described in any one of claim 1-7
Unicellular transfer chip and unicellular capture chip, the carrying channel entrance of the unicellular transfer chip and unicellular capture
The fluid course outlet of chip.
9. unicellular capture transfer system according to claim 8, it is characterised in that：In the unicellular transfer chip
The carrying channel entrance of each carrying channel goes out to the fluid course of the corresponding fluid course in the unicellular capture chip
Mouthful connection, it is described it is unicellular transfer chip in all carrying channels carrying channel outlet on the unicellular transfer chip
A corresponding carrier fluid outlet.
10. unicellular capture transfer system according to claim 8, it is characterised in that：The unicellular transfer chip with
Unicellular capture integrated chip setting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201721473021.0U CN207659420U (en) | 2017-11-07 | 2017-11-07 | Cleavable unicellular transfer chip and unicellular capture transfer system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721473021.0U CN207659420U (en) | 2017-11-07 | 2017-11-07 | Cleavable unicellular transfer chip and unicellular capture transfer system |
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| CN207659420U true CN207659420U (en) | 2018-07-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653220A (en) * | 2017-11-07 | 2018-02-02 | 苏州大学 | Unicellular capture transfer system and unicellular capture transfer method |
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2017
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653220A (en) * | 2017-11-07 | 2018-02-02 | 苏州大学 | Unicellular capture transfer system and unicellular capture transfer method |
| CN107653220B (en) * | 2017-11-07 | 2024-02-23 | 苏州大学 | Single cell capturing and transferring system and single cell capturing and transferring method |
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