CN202330222U - Microfluidic chip for granule and cell fixation - Google Patents
Microfluidic chip for granule and cell fixation Download PDFInfo
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- CN202330222U CN202330222U CN2011202550192U CN201120255019U CN202330222U CN 202330222 U CN202330222 U CN 202330222U CN 2011202550192 U CN2011202550192 U CN 2011202550192U CN 201120255019 U CN201120255019 U CN 201120255019U CN 202330222 U CN202330222 U CN 202330222U
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
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Abstract
The utility model provides a microfluidic chip for granule and cell fixation. The microfluidic chip comprises a wide-segment microflow pipeline and a narrow-segment microflow pipeline, which are connected, wherein the transverse section of the wide-segment microflow pipeline is trapezoidal, the transverse section of the narrow-segment microflow pipeline is triangular, and the height of the transverse section of the wide-segment microflow pipeline is larger than that of the transverse section of the narrow-segment microflow pipeline. Granules or cells can be conveyed to the chip by the wide-segment microflow pipeline, and are fixed because the sizes of the granules or cells are a little larger than that of the narrow-segment microflow pipeline. According to the utility model, the narrow-segment microflow pipeline adopts a sharp-top transverse section design, thus the shortcoming that the traditional flat-top narrow micro pipeline made from a dimethyl polysiloxane material is easy to collapse and seal under the compression of an external force is effectively overcome, and the microfluidic chip has great resistance to the external force and is stable and reliable.
Description
Technical field
The utility model relates to the microflow control technique field, is specifically related to a kind of micro-fluidic chip that is used for granulopexy and cell fixation.
Background technology
Granulopexy and cell be fixed on analytical chemistry and field of biological detection all has application potential quite widely.With analytical chemistry is example, and fixedly silica gel particle can carry out the size exclusion chromatograph analysis on micro-fluidic chip; The fixing microballon of processing through different surface treatment can be made the microballon microarray on micro-fluidic chip.In biological detection; Fixed cell helps pair cell and monitors in real time on micro-fluidic chip; Guarantee carrying out smoothly of dosage and temporal correlation experiment, and can realize unicellular controlling, thus the biological heterogeneous data that acquisition is difficult to obtain in the traditional experiment means etc.
The prior art employing is processed by dimethyl silicone polymer, square section is that the micro-fluidic pipeline of square (also can be referred to as flat-top) carries out granulopexy and cell fixation on micro-fluidic chip, and be specially: utilizing the formed dam structure fixed volume of flat-top micro-architecture through Twi-lithography process generation differing heights is big particle and cell than dam.
In research and practice process to prior art; The inventor of the utility model finds; Because the elasticity of dimethyl silicone polymer self; Micro-fluidic pipeline collapses receiving under the external force pressure that irreversible pipeline takes place easily, and the long and narrow micro-fluidic pipeline that especially highly is lower than 5 microns more possibly only cause collapsing because of faint electrostatic attraction under the external pressure situation not receiving.
The utility model content
One of technical matters that the utility model will solve provides a kind of micro-fluidic chip that is used for granulopexy and cell fixation; A kind of cross section narrow section miniflow pipeline configuration in pinnacle that have is provided, to solve the problem that the narrow miniflow pipeline of traditional flat-top shape sinks easily.
A kind of micro-fluidic chip that is used for granulopexy and cell fixation that the utility model provides; Comprise: wide section miniflow pipeline and narrow section miniflow pipeline; Said wide section miniflow pipeline is connected with said narrow section miniflow pipeline mutually; The square section of wherein said wide section miniflow pipeline is trapezoidal, and the square section of said narrow section miniflow pipeline is a triangle, and the cross section height of said wide section miniflow pipeline is greater than the cross section height of said narrow section miniflow pipeline.
The micro-fluidic chip that is used for granulopexy and cell fixation that the utility model provides; Comprise: wide section miniflow pipeline, narrow section miniflow pipeline group and sample cell; Said sample cell links to each other with said narrow section miniflow pipeline group, and said sample cell also links to each other with said wide section miniflow pipeline.
Can find out from above technical scheme; The embodiment of the invention has the following advantages: the miniflow pipeline adopts the design of square section, pinnacle; The narrow microchannel of flat-top shape that improves tradition effectively and with the dimethyl silicone polymer be material is easily in the shortcoming that receives to decay and seal under the external force pressure, for a kind of external force had high resistance and the reliable and stable particle or the fixedly microstructure of cell.This micro-fluidic chip have make simple, easy to operate, volume is little and advantage such as microminiaturization.
The micro-fluidic chip that the utility model provides can be integrated with existing various micro-fluidic chips, through fixing neurocyte oncocyte, to realize paralytic shellfish poisoning (PSP) detection new technology.
Description of drawings
Fig. 1 is a kind of structural drawing that is used for the micro-fluidic chip of granulopexy and cell fixation that the utility model embodiment provides;
The square section structural drawing of wide section miniflow pipeline of Fig. 2 is that the utility model embodiment provides a kind of micro-fluidic chip that is used for granulopexy and cell fixation;
The square section structural drawing of narrow section miniflow pipeline of Fig. 3 is that the utility model embodiment provides a kind of micro-fluidic chip that is used for granulopexy and cell fixation;
Fig. 4 is the structural drawing that another kind that the utility model embodiment provides is used for the micro-fluidic chip of granulopexy and cell fixation.
Embodiment
The utility model embodiment provides a kind of micro-fluidic chip that is used for granulopexy and cell fixation, below is elaborated.
Embodiment one, please with reference to Fig. 1, the utility model embodiment provides a kind of micro-fluidic chip that is used for granulopexy and cell fixation.
Granulopexy can be made up of two sections different miniflow miniflow pipelines of width with the cell fixation structure.As shown in Figure 1, wherein wide section miniflow pipeline 1 (hereinafter to be referred as wide segment pipe 1) is connected with narrow section miniflow pipeline 2 (hereinafter to be referred as narrow segment pipe 2) mutually, and wide segment pipe 1 is all inequality with the height and the transverse cross-sectional shape of narrow segment pipe 2.Like Fig. 2 and shown in Figure 3, wide segment pipe 1 and narrow segment pipe 2 have trapezoidal 3 and the square section structure of triangle 4 respectively, and narrow segment pipe 2 height of the aspect ratio of wide segment pipe 1.Particle or cell can be transported in the chip via wide segment pipe 1, and are fixed greatly than narrow segment pipe 2 because of size.Wide segment pipe 1 and narrow segment pipe 2 are that full dose is filtered configuration in line.The width of wide segment pipe 1 is 4 times of narrow segment pipe 2 width.The height that the square section is the wide segment pipe of flat-top trapezoidal 3 is 15 μ m, is 6 times of the square section narrow segment pipe height that is pinnacle triangle 4.Wide segment pipe 1 is to be formed by same printed circuit board stencil duplicating with narrow segment pipe 2, and the miniflow pipeline configuration of different depth is through the photomask striped of design different in width on this masterplate, produces through isotropic etching.Optional, the micro-fluidic chip that the utility model embodiment provides can be used the dimethyl silicone polymer injection mo(u)lding.
Embodiment two, as shown in Figure 4, the utility model embodiment also provide a kind of micro-fluidic chip that is used for granulopexy and cell fixation.
The micro-fluidic chip that is used for granulopexy and cell fixation can be designed as sample cell 5 and links to each other with narrow section miniflow pipeline group 6 (hereinafter to be referred as narrow segment pipe group 6); And wide section miniflow pipeline 8 (hereinafter to be referred as wide segment pipe 8) links to each other with sample cell 5; The both sides of sample cell 5 are provided with two rows totally eight narrow segment pipe groups 6, are the cross-flow filtration configuration.Described micro-fluidic chip is to be formed by same printed circuit board stencil duplicating, and the pipeline configuration of different depth is through the photomask striped of design different in width on this masterplate, produces through isotropic etching.Optional, the micro-fluidic chip that the utility model embodiment provides is to use the dimethyl silicone polymer injection mo(u)lding.Size is less than wide segment pipe 8 but can from left to right pass in and out sample cell 5 via wide segment pipe 8 greater than the particle of narrow segment pipe group 6.When wide segment pipe 8 ends apply normal pressure; Dispose based on cross-flow filtration; It is other that part particle or cell are transported to narrow segment pipe group 6 by current, and because of the wherein one dimension size of particle or cell is fixed greatly than narrow segment pipe group 6, current then can leave chip through narrow segment pipe group 6.
The method of utilizing narrow segment pipe group 6 fixed cells and realizing dyeing: leniently segment pipe 8 adds the HBSS buffer solution of 10 μ M calcein AM dyestuffs; Liquid level difference with inlet and outlet 10 μ L drives dyestuff arrival sample cell 5, allows the interior leukaemia HL60 cell of cultivating of fluorochrome label pipeline 10 minutes.With the fluorescence co-focusing microscopic examination, wherein excitation wavelength is 488nm, and the transmitted wave optical filter is chosen as 505-530nm.Through the leukaemia of fluorescent dyeing, the cell state of successfully fixing in the narrow segment pipe group 6 of dyeing explanation is good.
More than a kind of micro-fluidic chip that is used for granulopexy and cell fixation that the utility model embodiment is provided carried out detailed introduction; Used concrete example among this paper the principle and the embodiment of the utility model are set forth, the explanation of above embodiment just is used to help to understand the method and the core concept thereof of the utility model; Simultaneously, for one of ordinary skill in the art, according to the thought of the utility model, the part that on embodiment and range of application, all can change, in sum, this description should not be construed as the restriction to the utility model.
Claims (4)
1. a micro-fluidic chip that is used for granulopexy and cell fixation is characterized in that, comprising:
Wide section miniflow pipeline and narrow section miniflow pipeline; Said wide section miniflow pipeline is connected with said narrow section miniflow pipeline mutually; The square section of wherein said wide section miniflow pipeline is trapezoidal; The square section of said narrow section miniflow pipeline is a triangle, and the cross section height of said wide section miniflow pipeline is greater than the cross section height of said narrow section miniflow pipeline.
2. the micro-fluidic chip that is used for granulopexy and cell fixation according to claim 1 is characterized in that: said wide section miniflow pipeline and said narrow section miniflow pipeline are to be formed by same printed circuit board stencil duplicating.
3. micro-fluidic chip that is used for granulopexy and cell fixation; It is characterized in that; Comprise: wide section miniflow pipeline, narrow section miniflow pipeline group and sample cell, said sample cell links to each other with said narrow section miniflow pipeline group, and said sample cell also links to each other with said wide section miniflow pipeline.
4. the micro-fluidic chip that is used for granulopexy and cell fixation according to claim 3 is characterized in that: described micro-fluidic chip is to use the dimethyl silicone polymer injection mo(u)lding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202550192U CN202330222U (en) | 2011-07-18 | 2011-07-18 | Microfluidic chip for granule and cell fixation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202550192U CN202330222U (en) | 2011-07-18 | 2011-07-18 | Microfluidic chip for granule and cell fixation |
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| Publication Number | Publication Date |
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| CN202330222U true CN202330222U (en) | 2012-07-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011202550192U Expired - Fee Related CN202330222U (en) | 2011-07-18 | 2011-07-18 | Microfluidic chip for granule and cell fixation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925337A (en) * | 2012-11-08 | 2013-02-13 | 武汉友芝友生物制药有限公司 | Microfluid cell capturing chip and manufacture method thereof |
| CN105403499A (en) * | 2015-12-31 | 2016-03-16 | 中国人民解放军第三军医大学第一附属医院 | Single cell channel unit for optical detection |
| CN109569753A (en) * | 2018-12-29 | 2019-04-05 | 北京工业大学 | A kind of micro-fluidic chip lane device of PM2.5 powder collection and experimental observation |
-
2011
- 2011-07-18 CN CN2011202550192U patent/CN202330222U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925337A (en) * | 2012-11-08 | 2013-02-13 | 武汉友芝友生物制药有限公司 | Microfluid cell capturing chip and manufacture method thereof |
| CN105403499A (en) * | 2015-12-31 | 2016-03-16 | 中国人民解放军第三军医大学第一附属医院 | Single cell channel unit for optical detection |
| CN109569753A (en) * | 2018-12-29 | 2019-04-05 | 北京工业大学 | A kind of micro-fluidic chip lane device of PM2.5 powder collection and experimental observation |
| CN109569753B (en) * | 2018-12-29 | 2021-02-26 | 北京工业大学 | Micro-fluidic chip channel device for PM2.5 particle collection and experimental observation |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120711 Termination date: 20160718 |
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| CF01 | Termination of patent right due to non-payment of annual fee |