CN105403499A - Single cell channel unit for optical detection - Google Patents
Single cell channel unit for optical detection Download PDFInfo
- Publication number
- CN105403499A CN105403499A CN201511028622.6A CN201511028622A CN105403499A CN 105403499 A CN105403499 A CN 105403499A CN 201511028622 A CN201511028622 A CN 201511028622A CN 105403499 A CN105403499 A CN 105403499A
- Authority
- CN
- China
- Prior art keywords
- unicellular
- runner
- window
- optical detection
- liquid outlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 25
- 230000003287 optical effect Effects 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 230000003139 buffering effect Effects 0.000 claims description 20
- 125000006850 spacer group Chemical group 0.000 claims description 20
- 239000012530 fluid Substances 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 15
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims description 6
- 239000004809 Teflon Substances 0.000 claims description 3
- 229920006362 Teflon® Polymers 0.000 claims description 3
- 229920001577 copolymer Polymers 0.000 claims description 3
- 125000004122 cyclic group Chemical group 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000003814 drug Substances 0.000 abstract description 9
- 229940079593 drug Drugs 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 23
- 229920000089 Cyclic olefin copolymer Polymers 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000012857 repacking Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N15/1436—Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optical Measuring Cells (AREA)
Abstract
The invention discloses a single cell channel unit for optical detection. The single cell channel unit comprises an upper window of a single cell channel and a lower window of the single cell channel, wherein the upper window of the single cell channel and the lower window of the single cell channel are fixedly connected; one end of a connecting surface of the lower window of the single cell channel and the upper window of the single cell channel is provided with a sample introduction groove, the other end of the connecting surface is provided with a liquid discharge groove, the sample introduction groove is connected with the liquid discharge groove through a plurality of passages located between the sample introduction groove and the liquid discharge groove and composed of sample channels and liquid outlet channels, and the upper window of the single cell channel is provided with a sample introduction inlet and a liquid outlet at positions corresponding to the sample introduction groove and the liquid discharge groove. The unit achieves the purpose of single cell channel by arranging the sample channels and the liquid outlet channels, can monitor influence of a drug on cells in real time, improves measuring precision and is simple in structure and convenient to operate.
Description
Technical field
The invention belongs to and measure the chemistry of material or the device of physical property, be specifically related to the unicellular flow passage device for optical detection.
Background technology
Cell is the base unit of biosome and vital movement, cell operation for the exploration of the research of eucaryotic cell structure and function, vital movement rule and essence, the Clinics and Practices of disease, the screening of medicine and design etc. all tool be of great significance.
When carrying out the optical detection of cell, often can run into requirement and detecting in a row unicellular, allow it grow up to equally distributed state in a row because the growth inoculating rear cell is difficult to control.Therefore invention one can make cell uniform rows distribute, tight, and the optical flow duct device without superposition is necessary very much.Such as when the tera-hertz spectra carrying out cell detects, because tera-hertz spectra is to the strong absorption of water, cell is in a row distributed in runner carry out to detect the terahertz light spectrum signal that greatly can strengthen cell so filter out aqueous solution.
When carrying out the process experiment to cell such as medicine, this device can same group of cell before and after detection of drugs process, also can be used for research several drugs and produce which kind of impact respectively to cell simultaneously or successively; Also by repacking an injection port into several injection port, realize to medicine to a few class cell, or several drugs detects the parallel analysis that cell lineage affects.
Summary of the invention
In view of this, the object of the present invention is to provide the unicellular flow passage device for optical detection, structure is simple, cheap, this device can meet the object of the unicellular runner of multiple optical detection, can the impact on cell such as Real-Time Monitoring medicine, improves measuring accuracy.
For achieving the above object, the invention provides following technical scheme:
For the unicellular flow passage device of optical detection, comprise the upper window 5 of unicellular runner and the lower window 6 of unicellular runner, the upper window 5 of described unicellular runner is connected by stationary installation with the lower window 6 of unicellular runner, the lower window 6 of described unicellular runner is provided with sample introduction tank 61 with joint face one end of the upper window 5 of described unicellular runner, the other end is provided with liquid outlet groove 64, by expanding channels that several are made up of sample flow channel 62 and fluid runner 63 between described sample introduction tank 61 and liquid outlet groove 64, the corresponding described sample introduction tank 61 of upper window 5 of described unicellular runner and liquid outlet groove 64 position are respectively arranged with injection port 51 and liquid outlet 52.
In the present invention, correspondence position is provided with the upper mounted plate 1 of light source hole to described stationary installation and bottom plate 2 forms, and fixed plate 1 and bottom plate 2 to be laid respectively at outside the described fixing upper window 5 of unicellular runner and the lower window 6 of unicellular runner and the described fixing upper window 5 of unicellular runner and the lower window 6 of unicellular runner fixed by fixed leg.
Further, described fixing between the upper window 5 of unicellular runner and upper mounted plate 1 is also provided with buffering spacer 3; Also be provided with lower buffering spacer 4 between the lower window 62 of described unicellular runner and bottom plate 2, correspondence position is provided with light source hole for described upper buffering spacer 3 and lower buffering spacer 4 and with the light source hole of upper mounted plate 1 and bottom plate 2 on the same line.
Further, be parallel to each other between described sample flow channel 62, be parallel to each other between described fluid runner 63.
Further, the diameter of described injection port 51 and liquid outlet 52 is all set between 0.5 ~ 3mm, is preferably 2mm.
Further, the degree of depth of described sample introduction tank 61, liquid outlet groove 64, sample flow channel 62 and fluid runner 63 is 10-20 μm, is preferably 20 μm.
Further, the width of described sample flow channel 62 is 10-20 μm, and the width of described fluid runner 63 is 2 μm-10 μm, is preferably 5 μm.
Further, the material of the upper window 5 of described unicellular runner adopts and does not absorb or absorb less detection light and be easy to process satisfactory material, is preferably cyclic olefine copolymer; Fixed plate 1 and bottom plate 2 select material to be hard metal, are preferably stainless steel; The material of described upper buffering spacer 3 and lower buffering spacer 4 adopts the plastics with certain pliability, is preferably Teflon.
Beneficial effect of the present invention is: the invention discloses the unicellular flow passage device for optical detection, this apparatus structure is simple, cheap, upper and lower window can change material according to the detection light adopted, therefore the unicellular flow-paths inspection with multiple optics is used, easy to operate, interchangeability is strong, and testing result is reliable; And this device can realize the Real-Time Monitoring of medicine to impact cell, improves accuracy of detection, significant to clinical detection.
Accompanying drawing explanation
In order to make object of the present invention, technical scheme and beneficial effect clearly, the invention provides following accompanying drawing:
Fig. 1 is the structural representation (on 1-upper mounted plate, 2-bottom plate, 3-under buffering spacer, 4-the upper window of buffering spacer, the unicellular runner of 5-, the lower window of the unicellular runner of 6-) of unicellular flow passage device for optical detection.
Fig. 2 is the structural representation (61-sample introduction tank, 64-liquid outlet groove) of window under unicellular runner.
Fig. 3 is the structural representation (62-sample flow channel, 63-fluid runner) of window under unicellular runner.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.The experimental technique of unreceipted actual conditions in embodiment, the usually conveniently conditioned disjunction condition of advising according to manufacturer.
For the unicellular flow passage device of optical detection, structure as shown in Figure 1, comprise the upper window 5 of unicellular runner and the lower window 6 of unicellular runner, the upper window 5 of described unicellular runner is connected by stationary installation with the lower window 6 of unicellular runner, the lower window 6 of described unicellular runner is provided with sample introduction tank 61 with joint face one end of the upper window 5 of described unicellular runner, and the other end is provided with liquid outlet groove 64 (Fig. 2); By the expanding channels (Fig. 3) that several are made up of sample flow channel 62 and fluid runner 63 between described sample introduction tank 61 and liquid outlet groove 64, the corresponding described sample introduction tank 61 of upper window 5 of described unicellular runner and liquid outlet groove 64 position are respectively arranged with injection port and liquid outlet; In the present invention, stationary installation preferably correspondence position is provided with the upper mounted plate 1 of light source hole and bottom plate 2 forms, and fixed plate 1 and bottom plate 2 to be laid respectively at outside the described fixing upper window 5 of unicellular runner and the lower window 6 of unicellular runner and the described fixing upper window 5 of unicellular runner and the lower window 6 of unicellular runner fixed by fixed leg.In order to can better fix, between the upper window 5 of described fixing unicellular runner and upper mounted plate 1, be also provided with buffering spacer 3; Also be provided with lower buffering spacer 4 between the lower window 62 of described unicellular runner and bottom plate 2, correspondence position is provided with light source hole for described upper buffering spacer 3 and lower buffering spacer 4 and with the light source hole of upper mounted plate 1 and bottom plate 2 on the same line.
As the preferred embodiment of the invention, be parallel to each other between described sample flow channel 62, be parallel to each other between described fluid runner 63.
In order to can control sample introduction speed and go out sample speed, by the control of feed rate, the diameter of injection port and liquid outlet is set to 0.5 ~ 3mm, is preferably 2mm.
In the present embodiment, sample introduction tank 61, sample flow channel 62, the degree of depth of fluid runner 63 and liquid outlet groove 64 is set to 10-20 μm, is preferably 20 μm; The number of sample flow channel 62 is determined according to the size of hot spot, is preferably 500; In the present embodiment, sample introduction tank 61 is preferably the length of side is 2cm equilateral triangle, and liquid outlet groove 64 is the rectangle of 2 × 0.5cm.
In the present embodiment, the width of described sample flow channel 62 is 10 ~ 20 μm, is preferably 20 μm; The width of fluid runner 63 is 2 ~ 10 μm, is preferably 5 μm; The degree of depth to adjustment fluid runner 63 is 5 μm, can strengthen fluid runner 63 to width 20 μm.
For convenience of detecting, upper window 5 material of unicellular runner does not absorb according to employing detection light or absorbs few material, according to Terahertz light source, the material of the upper window 5 of unicellular runner can adopt cyclic olefine copolymer to be called for short COPs (CycloOlefinPolymers) or COCs (CycloOlefinCopolymers).The material of upper mounted plate 1 and bottom plate 2 is to select hard metal, adopts stainless steel in the present embodiment; The material of upper buffering spacer 3 and lower buffering spacer 4 is the plastics with certain pliability, adopts Teflon in the present embodiment.
In the present embodiment, sample introduction tank 61, sample flow channel 62, fluid runner 63, liquid outlet groove 64 is selected to carry out to etch according to the material of lower window 6, hot-forming or moldings formed therefrom; If the material of lower window 6 is silicon chip, groove adopts etching technics.
What finally illustrate is, above preferred embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although by above preferred embodiment to invention has been detailed description, but those skilled in the art are to be understood that, various change can be made to it in the form and details, and not depart from claims of the present invention limited range.
Claims (8)
1. for the unicellular flow passage device of optical detection, it is characterized in that: comprise the upper window of unicellular runner and the lower window of unicellular runner, the upper window of described unicellular runner is connected by stationary installation with the lower window of unicellular runner, joint face one end of the lower window of described unicellular runner and the upper window of described unicellular runner is provided with sample introduction tank, the other end is provided with liquid outlet groove, by several expanding channels be made up of sample flow channel and fluid runner between described sample introduction tank and liquid outlet groove, the corresponding described sample introduction tank of upper window of described unicellular runner and liquid outlet groove position are respectively arranged with injection port and liquid outlet.
2. according to claim 1 for the unicellular flow passage device of optical detection, it is characterized in that: correspondence position is provided with the upper mounted plate of light source hole to described stationary installation and bottom plate forms, fixed plate and bottom plate to be laid respectively at outside the described fixing upper window of unicellular runner and the lower window of unicellular runner and the described fixing upper window of unicellular runner and the lower window of unicellular runner are fixed by fixed leg.
3. according to claim 2 for the unicellular flow passage device of optical detection, it is characterized in that: between the upper window of described fixing unicellular runner and upper mounted plate, be also provided with buffering spacer; Also be provided with lower buffering spacer between the lower window of described unicellular runner and bottom plate, correspondence position is provided with light source hole for described upper buffering spacer and lower buffering spacer and with the light source hole of upper mounted plate and bottom plate on the same line.
4. according to claim 1 for the unicellular flow passage device of optical detection, it is characterized in that: be parallel to each other between described sample flow channel, be parallel to each other between described fluid runner.
5., according to claim 1 for the unicellular flow passage device of optical detection, it is characterized in that: the diameter of described injection port and liquid outlet is all set to 0.5 ~ 3mm.
6. according to claim 1 for the unicellular flow passage device of optical detection, it is characterized in that: the degree of depth of described sample introduction tank, liquid outlet groove, sample flow channel and fluid runner is 10-20 μm.
7. according to claim 1 for the unicellular flow passage device of optical detection, it is characterized in that: the width of described sample flow channel is 10-20 μm, the width of described fluid runner is 2 μm-10 μm.
8. according to claim 1 for the unicellular flow passage device of optical detection, it is characterized in that: the material of the upper window of described unicellular runner is cyclic olefine copolymer; Fixed plate and bottom plate are stainless steel; The material of described upper buffering spacer and lower buffering spacer is Teflon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511028622.6A CN105403499A (en) | 2015-12-31 | 2015-12-31 | Single cell channel unit for optical detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511028622.6A CN105403499A (en) | 2015-12-31 | 2015-12-31 | Single cell channel unit for optical detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105403499A true CN105403499A (en) | 2016-03-16 |
Family
ID=55469124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511028622.6A Pending CN105403499A (en) | 2015-12-31 | 2015-12-31 | Single cell channel unit for optical detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105403499A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1987420A (en) * | 2006-12-30 | 2007-06-27 | 清华大学 | Microfluidic chip device for multifunctional detection of single particulate matter |
| CN101343656A (en) * | 2008-08-22 | 2009-01-14 | 重庆大学 | Cell separation microchip based on silicon structure on insulators |
| CN202330222U (en) * | 2011-07-18 | 2012-07-11 | 深圳市检验检疫科学研究院 | Microfluidic chip for granule and cell fixation |
| CN102796667A (en) * | 2012-08-21 | 2012-11-28 | 北京大学 | Microfluidic chip allowing different cell-density arrangement and use thereof |
| WO2012162181A2 (en) * | 2011-05-20 | 2012-11-29 | The Brigham And Women's Hospital, Inc. | Analysis and sorting of motile cells |
| CN104502303A (en) * | 2015-01-20 | 2015-04-08 | 中国人民解放军第三军医大学第一附属医院 | Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof |
| CN103361263B (en) * | 2013-07-11 | 2015-05-20 | 北京大学 | Cell chemotaxis analysis chip and device as well as use method and preparation method of cell chemotaxis analysis chip |
| CN105203468A (en) * | 2015-10-19 | 2015-12-30 | 中国科学院合肥物质科学研究院 | Micro-fluidic chip system for improving radiation flux of single ion beam |
-
2015
- 2015-12-31 CN CN201511028622.6A patent/CN105403499A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1987420A (en) * | 2006-12-30 | 2007-06-27 | 清华大学 | Microfluidic chip device for multifunctional detection of single particulate matter |
| CN101343656A (en) * | 2008-08-22 | 2009-01-14 | 重庆大学 | Cell separation microchip based on silicon structure on insulators |
| WO2012162181A2 (en) * | 2011-05-20 | 2012-11-29 | The Brigham And Women's Hospital, Inc. | Analysis and sorting of motile cells |
| CN202330222U (en) * | 2011-07-18 | 2012-07-11 | 深圳市检验检疫科学研究院 | Microfluidic chip for granule and cell fixation |
| CN102796667A (en) * | 2012-08-21 | 2012-11-28 | 北京大学 | Microfluidic chip allowing different cell-density arrangement and use thereof |
| CN103361263B (en) * | 2013-07-11 | 2015-05-20 | 北京大学 | Cell chemotaxis analysis chip and device as well as use method and preparation method of cell chemotaxis analysis chip |
| CN104502303A (en) * | 2015-01-20 | 2015-04-08 | 中国人民解放军第三军医大学第一附属医院 | Sub-THz nano-biosensor for quickly frame-detecting bacteria and detection method thereof |
| CN105203468A (en) * | 2015-10-19 | 2015-12-30 | 中国科学院合肥物质科学研究院 | Micro-fluidic chip system for improving radiation flux of single ion beam |
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| Title |
|---|
| JINHONG GUO 等: "Precise Enumeration of Circulating Tumor Cells Using Support Vector Machine Algorithm on a Microfluidic Sensor", 《IEEE TRANSACTIONS ON EMERGING TOPICS IN COMPUTING》 * |
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Application publication date: 20160316 |