CN105647789B - A kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip - Google Patents
A kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip Download PDFInfo
- Publication number
- CN105647789B CN105647789B CN201610066768.8A CN201610066768A CN105647789B CN 105647789 B CN105647789 B CN 105647789B CN 201610066768 A CN201610066768 A CN 201610066768A CN 105647789 B CN105647789 B CN 105647789B
- Authority
- CN
- China
- Prior art keywords
- channel
- micro
- fluidic chip
- real
- component
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
Abstract
The present invention provides a kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip, comprising: micro-fluidic chip, automatic sampling component, pre-treatment and enrichment component and detector.The channel with cell culture chamber is provided in micro-fluidic chip.The automatic sampling component that can be sampled from the exit in channel is provided with around micro-fluidic chip.Processing and enrichment component, can carry out desalination to the products of cellular metabolism that sampling obtains and target product is enriched with before the downstream connection of automatic sampling component.Exit corresponding to pre-treatment and enrichment component is provided with detector, to implement on-line checking to target product.Detection device collection micro-fluidic chip of the invention, automatic sampling, pre-treatment and enrichment and be detected on one, can on micro-fluidic chip biochemical reaction and drug metabolism processes carry out real-time, online detection and analysis.
Description
Technical field
The present invention relates to cell research technical field more particularly to a kind of products of cellular metabolism based on micro-fluidic chip are real
When detection device.
Background technique
It is well known that microfluidic chip technology is relative to traditional cell research platform, due to low reagent consumption,
Easy of integration, high-throughput, easy to operate and good reproducibility and widely paid close attention to.In recent years, zoopery start by
Gradually opposed by the mankind, and microfluidic chip technology is then one of the method that zoopery can be replaced most potential, is had wide
Wealthy application prospect.Generally with the comparable micro scale channel of cell size, this to study people in channel in micro-fluidic chip
Member not only can accurately manipulate cell, additionally it is possible to reduce the consumption of cell dosage and reagent.In the prior art, utilization is micro-
The characteristic of fluidic chip successfully simulates growing state of the cancer in lactiferous ducts in the chips.
Drug metabolism is recognized that the structure of the molecule really to work in drug, this can play clinical medicine use
To guidance and supervisory function bit.In order to preferably carry out complicated biochemical reaction and drug metabolism simulation on micro-fluidic chip,
Need more perfect detection and analysis device and method.Traditional drug metabolism detection be it is non-online, due to not can be carried out
Continuous metabolism monitoring, this prevents research staff from comprehensively understanding cell to drug absorption, the overall process of metabolism.Therefore,
How to establish a analytical equipment and method that can be online, quick and brief process just seems non-for drug metabolism analysis
It is often important.In addition, detection and analysis device in the prior art is at product enrichment, detection limit and anti-interference aspect, there are still very
More deficiencies, this to carry out complex biochemical reaction and drug metabolism on micro-fluidic chip by biggish limitation.
Summary of the invention
The present invention provides a kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip, comprising: micro-fluidic
Chip, automatic sampling component, pre-treatment and enrichment component and detector.Band cell culture chamber is provided in micro-fluidic chip
Channel.The automatic sampling component that can be sampled from the exit in channel is provided with around micro-fluidic chip.In automatic sampling
The downstream connection pre-treatment of component and enrichment component can carry out desalination and target product to the products of cellular metabolism that sampling obtains
Enrichment.Exit corresponding to pre-treatment and enrichment component is provided with detector, to implement on-line checking to target product.At this
Kind in the case of, detection device collection micro-fluidic chip of the invention, automatic sampling, pre-treatment and enrichment and be detected on one, can
With on micro-fluidic chip biochemical reaction and drug metabolism processes carry out real-time, online detection and analysis.I.e., it is possible to thin
Born of the same parents' metabolite is continuously monitored, comprehensively to understand the biochemical reaction on micro-fluidic chip and the full mistake of drug metabolism
Journey.
Further, micro-fluidic chip includes the channel of multiple parallel connections, the exit region in the channel close to multiple parallel connections
It is equipped with cell culture chamber.Preferably, the channel with dendroid bifurcated can be set into channel.Thus, it is possible to simultaneously, online
Carry out multiple groups experiment, realization the cell metabolism situation in multiple channels is continuously monitored and is compared, with reach to it is multiple carefully
The purpose that born of the same parents' metabolism or biochemical reaction are monitored and detect.
Further, micro-fluidic chip further includes the flushing channel equipped with cleaning solution storage room.As a result, when automatic sampling group
Part can first clean automatic sampling component using flushing channel, before sampling in different channels to avoid difference
Interfering with each other between channel.
Further, the sampling outwardly protruded perpendicular to channel is configured in the outlet end of parallel port and flushing channel
Mouthful.Preferably, the shape of sample tap can be configured to match with automatic sampling component, to facilitate sampling.
Further, automatic sampling component includes pipette, mobile platform and pump.One end of pipette is logical can be aligned
The mode of the sample tap in road is set in mobile platform, and the other end of pipette is connected to pump.As a result, in the control of computer software
Lower pipette can be sampled with above and below mobile platform and moving left and right with being directed at the different sample taps of micro-fluidic chip.
In this case, it is moved back and forth by mobile platform, pipette continuous, online, repeatedly can take from different sample taps
Sample.In addition.Under the action of pump, the micro-example of extraction can be pumped into pre-treatment and enrichment component, to carry out in next step
Treatment process.
Further, pre-treatment and enrichment component include enriching column and channel converter.The entrance of enriching column through conduit and
Channel converter can be connected to automatic sampling component；The outlet of enriching column can be connected to through conduit and channel converter with waste liquid bottle.
The sample sampled as a result, carries out being enriched with except salt treatment and target product first in enriching column, later by unwanted production
Object is discharged into waste liquid bottle.
Further, pre-treatment and enrichment component further include syringe pump and splitter.Syringe pump can pass through conduit and channel
Converter is connected to the entrance of enriching column；Splitter can pass through the outlet of conduit and channel converter and enriching column.As a result,
By syringe pump can be injected into enriching column eluent to target product carry out it is de- wash, later, target product enters splitter
The middle separation for carrying out classification, in order to subsequent detection.
Further, channel converter is six-way valve.The converted of channel may be implemented by rotating six-way valve as a result,
Journey, and then different pretreatment process is carried out to sample.
Further, detector is electrospray mass spectrometer or chromatograph.Preferably, the outlet of splitter can be with spraying with point
End connection.Thus, it is possible to carry out different detections to sample.
The present invention has the advantages that (1) directly can carry out on-line real-time measuremen to cell culture base fluid.(2) in target
Product enrichment aspect, detection limit and anti-interference aspect can preferably meet the needs of people.(3) it may be implemented to multiple channels
Cell metabolism situation be monitored and compare, convenient for carrying out multiple groups experiment simultaneously.(4) have higher detection sensitivity and
Broader applicability.
Detailed description of the invention
The invention will be described in more detail below based on embodiments and refering to the accompanying drawings.Wherein:
Fig. 1 is the structural representation of the products of cellular metabolism real-time detection apparatus based on micro-fluidic chip of the embodiment of the present invention
Figure；
Fig. 2 is the structural schematic diagram of the micro-fluidic chip of the embodiment of the present invention；
The schematic diagram of Fig. 3 a and the pre-treatment that 3b is the embodiment of the present invention and the different use states of enrichment component；And
Fig. 4 is the spraying partial structure diagram with tip and detector of the embodiment of the present invention.
In the accompanying drawings, identical component uses identical appended drawing reference.Attached drawing is not according to actual ratio.
Specific embodiment
The present invention will be further described with reference to the accompanying drawings.
As shown in figures 1 and 3, a kind of products of cellular metabolism real-time detection apparatus 100 based on micro-fluidic chip, comprising:
Micro-fluidic chip 6, automatic sampling component, pre-treatment and enrichment component 40 and detector 20.It is provided in micro-fluidic chip 6
Channel 7 with cell culture chamber 7 '.Being provided with around micro-fluidic chip 6 can taking automatically from the sampling of the exit in channel 7
Sample component.Processing and enrichment component 40, can produce the cell metabolism that sampling obtains before the downstream connection of automatic sampling component
Object carries out desalination and target product enrichment.Exit corresponding to pre-treatment and enrichment component 40 is provided with detector 20, with right
Target product implements on-line checking.In this case, detection device collection micro-fluidic chip 6 of the invention, automatic sampling, preceding place
Reason and enrichment and be detected on one, can on micro-fluidic chip 6 biochemical reaction and drug metabolism processes carry out in real time,
The detection and analysis of line.I.e., it is possible to continuously be monitored to products of cellular metabolism, comprehensively to understand on micro-fluidic chip 6
Biochemical reaction and drug metabolism overall process.
In a specific embodiment of the invention, as illustrated in fig. 1 and 2, micro-fluidic chip 6 is placed in removable loading
On platform 4.Removable article carrying platform 4 is connect with stage carrier 1.Micro-fluidic chip 6 includes the channel 7 of multiple parallel connections, preferably
Ground, channel 7 can be configured to dendroid.The exit structure that there are two chip inlets 2, in channel 7 is set in the inlet in channel 7
Make the sample tap 9 outwardly protruded perpendicular to channel.The cross sectional shape of sample tap 9 can be arbitrarily arranged, it is preferable that in order to suction
Liquid pipe 10 matches, and sample tap 9 is arranged to tubulose of the bore slightly larger than the bore of pipette 10.The main part in channel 7 constructs
At straight trip channel or serpentine channel, in addition, being provided with cell culture chamber 7 ' on the straight trip channel in channel 7.Preferably, cell is trained
Support the region that room 7 ' is positioned close to sample tap 9.
After injecting various concentration or drug containing and the not cell culture base fluid of drug containing from chip inlet 2, carefully
Born of the same parents' liquid medium forms certain concentration gradient through the main part in channel 7.The cell culture base fluid of various concentration enters cell
In culturing room 7 ', different stimulations is carried out to cell so that cell makes corresponding response, and then generates the change of metabolic activity,
The Related Component of the culture medium of surrounding is set to change.Different products of cellular metabolism is in base fluid by sample tap 9 by pipette
10 are drawn onto pre-treatment and enrichment component, to carry out subsequent processing.
In order to avoid interfering with each other between different channels 7, individual column channel is additionally provided on micro-fluidic chip 6,
That is, the flushing channel 8 with cleaning solution storage room, and it is furnished with corresponding inlet 3.In this case, when pipette 10 is not
Before being sampled between same channel 7, first pipette 10 can be cleaned using the liquid in flushing channel 8, with maximum limit
Avoid detection error to degree.
In a specific embodiment of the invention, as shown in Figure 1, automatic sampling component includes pipette 10, imbibition
Pipe fixed platform 5 and pump 12.Mobile platform 5 is connect with stage carrier 1, and is set to the top of micro-fluidic chip 6.Imbibition
Pipe 10 is vertically arranged in mobile platform 4, can be directed at the sample tap 9 in channel 7.The other end of pipette 10 by conduit 17 with
12 connection of pump.In this case, by the control of computer software, pipette 10 can be moved with about 5 mobile platform and left and right
It is dynamic, it is sampled or cleans to be directed at the different sample taps 9 of micro-fluidic chip 6.By the reciprocating motion of stage carrier 1,
Pipette 10 can be sampled from different sample taps 9 continuously, online, repeatedly.In addition, can will be mentioned under the action of pumping 12
The micro-example taken is pumped into pre-treatment and enrichment component 40, to carry out the treatment process of next step.
In a specific embodiment of the invention, as shown in figures 1 and 3, pre-treatment and enrichment component 40 mainly by
14 enriching column of six-way valve, 16 syringe pump 15 and splitter 18 at.Six-way valve 14 will play the role of channel conversion.For the ease of saying
Bright, the hole that six-way valve 14 is connected with pump is defined as No.1 hole by we, successively adjacent with No.1 hole along rotation counterclockwise to be
No. two holes to No. six holes.When six-way valve 14 rotates, as shown in Figure 3a, 16 entrance of enriching column is connected to by conduit with No. two holes,
No. two holes are connected to No.1 hole and are finally connected to automatic sampling component 30.The outlet of enriching column 16 is connected by conduit and No. five holes
Logical, No. five holes are connected to No. six holes and are ultimately connected to waste liquid bottle 17.After valve 14 rotates counterclockwise, as shown in Figure 3b, at this point, rich
16 entrance of clustered column is connected to by conduit with No. two holes, and No. two holes are connected to No. three holes, and No. three holes connect 15 enriching column 16 of syringe pump
Outlet is connected to by conduit with No. five holes, and No. five holes are connected to No. four holes, and No. four holes connect splitter 18
When six-way valve 14 rotates, the cell culture base fluid by pumping 12 samples enters through conduit 13 to 14 No.1 hole of six-way valve,
Then enter No. two holes by the channel between six-way valve 14, enter enriching column 16 through No. two holes.It is thin in cell culture base fluid
Born of the same parents' metabolite carries out desalination and target product enrichment in enriching column 16.The ingredient and liquid not being enriched with go out from enriching column 16
Mouth is ultimately discharged into waste liquid bottle 17 by conduit, through No. five holes and No. six holes.After 14 turns of six-way valve, pass through what is connect with No. three holes
Syringe pump 15 enters eluent, and the target product of enriching column 16 is eluted, and is connected to splitter 18 using No. four holes and carries out
Separation.
In a specific embodiment of the invention, as shown in figure 4, the same class target after the separation of splitter 18 produces
Object is flowed into spraying tip 19, high pressure is connected at mist tip 19, so that by spraying with the liquid at tip 19 to charge micro- liquid
The form of drop ejects, and enters in electrospray mass spectrometer 20 and is detected.
Micro-fluidic chip of the invention is made of the photolithography method of standard, and detailed process is as follows: firstly, by silicon wafer
It is heated in the mixed solution that the weight ratio for being put in the concentrated sulfuric acid and hydrogen peroxide is 3:1, slightly boiled about 30 minutes.Then, silicon wafer is washed
It completely and dries, spin coating is carried out to silicon wafer on sol evenning machine, the height in channel required for controlling.Later, the silicon that spin coating is good
Piece is put into progress front baking processing in baking oven, and holding temperature is 65 DEG C, and the time is 30 minutes.Using exposure machine by photo etched mask to this
Silicon wafer carries out uv-exposure, then silicon wafer is put into baking oven after progress and is dried, and holding temperature is 65 DEG C, and the time is 15 minutes or so,
So that required channel is further reinforced.After above-mentioned treatment process, development and silanization treatment are carried out to silicon wafer,
Required mold can be obtained.Mold is put into baking oven, extra developer and silylating reagent are evaporated.Finally,
Dimethyl silicone polymer (PDMS) and initiator are mixed with the weight ratio of 10:1, imported on mold, after degassing, is put into baking oven
Middle 4-6 hour of drying obtains the solid-like PDMS module with micro-fluidic chip channel.This PDMS module is taken off from silicon wafer
From, punched with punch, then with sheet glass carry out plasma be bonded after just obtain it is micro-fluidic used in the present invention
Chip.
In conclusion the products of cellular metabolism real-time detection apparatus of the invention based on micro-fluidic chip, by mobile flat
The movement of platform 5, can make the different sample taps 9 of the arrival micro-fluidic chip 6 of 10 controllable precise of pipette, and can adjust
Moving up and down for control pipette 10, enables pipette 10 closely to be connected and separated with sample tap 9.In the regulation of pump 12
Under, the culture medium that can draw designated volume is detected, then by subsequent enrichment, separation and detecting step, may finally
It is effectively obtained the mass spectrum of some substances or chromatography detection signal in culture medium.
Although by reference to preferred embodiment, invention has been described, the case where not departing from the scope of the present invention
Under, various improvement can be carried out to it and can replace component therein with equivalent.Especially, as long as there is no structures to rush
Prominent, items technical characteristic mentioned in the various embodiments can be combined in any way.The invention is not limited to texts
Disclosed in specific embodiment, but include all technical solutions falling within the scope of the claims.
Claims (7)
1. a kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip characterized by comprising
Micro-fluidic chip is provided with the channel with cell culture chamber；Micro-fluidic chip further includes clear with cleaning solution storage room
Wash channel；
Automatic sampling component is set to around the micro-fluidic chip, and can be sampled from the exit in the channel；
Pre-treatment and enrichment component, are connected to the downstream of the automatic sampling component, produce to the obtained cell metabolism that samples
Object carries out desalination and target product enrichment；And
The pre-treatment and enrichment component include enriching column and a channel converter；The entrance of the enriching column is through conduit and leads to
Road converter can be connected to the automatic sampling component；The outlet of the enriching column is through conduit and channel converter energy and waste liquid bottle
Connection；
The pre-treatment and enrichment component further include syringe pump and splitter；The syringe pump can pass through conduit and channel converter
It is connected to the entrance of the enriching column；The splitter can be connected by the outlet of conduit and channel converter and the enriching column
It is logical；
Detector is correspondingly arranged in the pre-treatment and is enriched with the exit of component, to implement online inspection to the target product
It surveys.
2. real-time detection apparatus according to claim 1, which is characterized in that the micro-fluidic chip includes multiple in parallel
Channel；
Exit region in the channel close to multiple parallel connections is equipped with cell culture chamber.
3. real-time detection apparatus according to claim 2, which is characterized in that channel and flushing channel in the parallel connection
Outlet end is configured with the sample tap outwardly protruded perpendicular to channel.
4. real-time detection apparatus according to claim 3, which is characterized in that the automatic sampling component include pipette,
Mobile platform and pump；
One end of the pipette is set in the mobile platform in a manner of it can be directed at the sample tap in the channel, the suction
The other end of liquid pipe is connected to the pump.
5. real-time detection apparatus according to claim 1, which is characterized in that the channel converter is six-way valve.
6. real-time detection apparatus according to claim 1, which is characterized in that the detector is electrospray mass spectrometer or color
Spectrometer.
7. real-time detection apparatus according to claim 6, which is characterized in that the outlet of the splitter can be with spraying with point
End connection.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610066768.8A CN105647789B (en) | 2016-01-29 | 2016-01-29 | A kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip |
| PCT/CN2017/072258 WO2017129099A1 (en) | 2016-01-29 | 2017-01-23 | Real-time cell metabolite detection device based on microfluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610066768.8A CN105647789B (en) | 2016-01-29 | 2016-01-29 | A kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105647789A CN105647789A (en) | 2016-06-08 |
| CN105647789B true CN105647789B (en) | 2019-01-04 |
Family
ID=56488140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610066768.8A Active CN105647789B (en) | 2016-01-29 | 2016-01-29 | A kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN105647789B (en) |
| WO (1) | WO2017129099A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105647789B (en) * | 2016-01-29 | 2019-01-04 | 清华大学 | A kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip |
| CN109682902B (en) * | 2018-12-19 | 2021-12-03 | 中国科学院成都生物研究所 | Micro-fluidic chip, extraction type micro-fluidic chip-mass spectrometry combined analysis device and method |
| WO2021026261A1 (en) * | 2019-08-05 | 2021-02-11 | Auer Precision Company, Inc. | Microfluidic passive plasma separation device and method |
| CN111172025B (en) * | 2020-01-14 | 2022-11-15 | 中国科学院烟台海岸带研究所 | Compound micro-fluidic chip device of hydrogen sulfide activity detection based on cell |
| WO2022226920A1 (en) * | 2021-04-29 | 2022-11-03 | 京东方科技集团股份有限公司 | Immunoassay device and immunoassay method |
| CN114507593B (en) * | 2022-01-29 | 2023-10-17 | 扬州大学 | Integrated portable drug sensitive detection chip |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004062801A1 (en) * | 2003-01-14 | 2004-07-29 | Diagnoswiss S.A. | Multi-layered electrochemical microfluidic sensor comprising reagent on porous layer |
| CN201876442U (en) * | 2010-11-11 | 2011-06-22 | 复旦大学 | Seamless connection device for detecting antibiotics in milk on line |
| CN103226127A (en) * | 2013-03-27 | 2013-07-31 | 清华大学 | Multi-channel micro-fluidic chip and mass spectrum combined device |
| CN203191360U (en) * | 2013-04-28 | 2013-09-11 | 镇江出入境检验检疫局检验检疫综合技术中心 | On-line enrichment device for qualitatively and quantificationally measuring additive ingredients in food by ion chromatograph mass spectrometer |
| CN104483434A (en) * | 2014-11-29 | 2015-04-01 | 复旦大学 | Automatic analytical system and automatic analytical method for proteomes of less than or equal to 100 cells |
| CN104569329A (en) * | 2014-12-23 | 2015-04-29 | 天津大学 | Combination drug high-throughput screening system based on micro fluidic device and operation method |
| CN104698064A (en) * | 2015-03-18 | 2015-06-10 | 青岛大学 | Multichannel microfluidic-solid phase extraction-mass spectrum combined device and preparation method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7767435B2 (en) * | 2003-08-25 | 2010-08-03 | University Of Washington | Method and device for biochemical detection and analysis of subcellular compartments from a single cell |
| US20060285999A1 (en) * | 2005-06-21 | 2006-12-21 | West Virginia University Research Corporation | Apparatus and method for coupling microfluidic systems with electrospray ionization mass spectrometry utilizing a hydrodynamic flow restrictor |
| US8815179B2 (en) * | 2010-12-03 | 2014-08-26 | Alfa Wassermann, Inc. | Automated aseptic liquid collection workstations and collection devices therefore |
| CN103725747A (en) * | 2014-01-26 | 2014-04-16 | 佟雪梅 | Identification method and equipment for sensibility of bacteria on antibiotic |
| CN104560715B (en) * | 2015-01-31 | 2016-09-07 | 重庆大学 | Cultivate based on micro-fluidic chip and the method for detection lung carcinoma cell |
| CN105647789B (en) * | 2016-01-29 | 2019-01-04 | 清华大学 | A kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip |
-
2016
- 2016-01-29 CN CN201610066768.8A patent/CN105647789B/en active Active
-
2017
- 2017-01-23 WO PCT/CN2017/072258 patent/WO2017129099A1/en active Application Filing
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004062801A1 (en) * | 2003-01-14 | 2004-07-29 | Diagnoswiss S.A. | Multi-layered electrochemical microfluidic sensor comprising reagent on porous layer |
| CN201876442U (en) * | 2010-11-11 | 2011-06-22 | 复旦大学 | Seamless connection device for detecting antibiotics in milk on line |
| CN103226127A (en) * | 2013-03-27 | 2013-07-31 | 清华大学 | Multi-channel micro-fluidic chip and mass spectrum combined device |
| CN203191360U (en) * | 2013-04-28 | 2013-09-11 | 镇江出入境检验检疫局检验检疫综合技术中心 | On-line enrichment device for qualitatively and quantificationally measuring additive ingredients in food by ion chromatograph mass spectrometer |
| CN104483434A (en) * | 2014-11-29 | 2015-04-01 | 复旦大学 | Automatic analytical system and automatic analytical method for proteomes of less than or equal to 100 cells |
| CN104569329A (en) * | 2014-12-23 | 2015-04-29 | 天津大学 | Combination drug high-throughput screening system based on micro fluidic device and operation method |
| CN104698064A (en) * | 2015-03-18 | 2015-06-10 | 青岛大学 | Multichannel microfluidic-solid phase extraction-mass spectrum combined device and preparation method |
Non-Patent Citations (3)
| Title |
|---|
| Online multi-channel microfluidic chip-mass spectrometry and its application for quantifying noncovalent protein–protein interactions;Wu Liu等;《Analyst》;20150106;第140卷(第5期);第1551页摘要，图1及其说明 |
| Real-Time Cellular Exometabolome Analysis with a Microfluidic-Mass Spectrometry Platform;Christina C. Marasco等;《Plos One》;20150227;第10卷(第2期);第3页最后1段，第4页第1-2段，第8页最后1段，图3及其说明 |
| 基于微流控芯片-质谱联用的细胞分析研究进展;李海芳等;《中国科学: 化学》;20140403;第44卷(第5期);第777-783页 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105647789A (en) | 2016-06-08 |
| WO2017129099A1 (en) | 2017-08-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105647789B (en) | A kind of products of cellular metabolism real-time detection apparatus based on micro-fluidic chip | |
| US11045806B2 (en) | Integrated type microfluidic electrochemical biosensor system and method for rapid biochemical analysis | |
| CN108686721B (en) | Micro-fluidic chip for separating and detecting whole blood sample and detection method thereof | |
| CN103226127B (en) | Multi-channel micro-fluidic chip and mass spectrum combined device | |
| CN104502617A (en) | Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue | |
| CN109765163B (en) | Integrated analysis system and method for combining droplet microfluidics with mass spectrometry | |
| CN102650601A (en) | Microfluidic separation of plasma for colormetric assay | |
| CN103508411A (en) | Preparation method of microfluidic monolithic column chip and application of chip in raman detection | |
| TWI411779B (en) | Microfluidic bio-chip and automatic reaction detection system thereof | |
| CN103801412A (en) | Integrated micro-fluidic chip used for fluorescence detection of enzyme catalysis product and application thereof | |
| CN101382490A (en) | Method for screening high content medicament of cellular level | |
| CN106290269B (en) | A kind of compound microbial rapid detection method and microchip systems | |
| CN106591105A (en) | Microorganism automatic sample introduction system based on microfluidic chip and preparation method thereof | |
| CN104001562A (en) | Disc centrifugation type micro-fluidic chip and device for rapid chromium form separation | |
| CN205719981U (en) | A kind of SERS flexible biological probe based on nanometer silver array | |
| Chen et al. | Integrated microfluidic chip coupled to mass spectrometry: A minireview of chip pretreatment methods and applications | |
| CN109482249B (en) | Microfluidic device and method for detecting high molecular content in non-Newtonian fluid | |
| CN106940351A (en) | A kind of method of detection traditional Chinese medicine quality and its bioactive ingredients based on micro-fluidic chip | |
| CN104307583A (en) | Chip-scale gas-liquid separator and control system thereof | |
| CN106345541A (en) | Diphasic liquid-liquid extraction and electrophoretic separation integration microfluidic device and method | |
| CN109520993A (en) | Foodstuff surface residue micro-fluidic detection device and method based on Raman spectrum | |
| CN207130273U (en) | A kind of magnetic force micro-fluidic chip for capturing bacterium | |
| CN203164043U (en) | Sample preparation instrument | |
| CN107179378B (en) | Liquid phase chromatography analytical method that is a kind of while detaching metabolism group and lipid group component and its application | |
| CN207851078U (en) | A kind of immune micro-fluidic detection card |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |