CN209636230U - Micro-fluidic chip and the device for capturing simple target object - Google Patents
Micro-fluidic chip and the device for capturing simple target object Download PDFInfo
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Abstract
The utility model relates to micro-fluidic fields, the in particular to device of micro-fluidic chip and capture simple target object.The micro-fluidic chip includes substrate 1, and substrate is provided with injection port 4, outlet 5, fluid channel 2 and shape for hat chamber 3；Shape for hat chamber 3 is set between injection port 4, outlet 5, and the opening of shape for hat chamber 3 is connected to fluid channel 2.The micro-fluidic chip has special shape for hat chamber structure, each chamber is all small detection and analysis room, the length of chamber longitudal section is greater than object droplet (or cell), and the length of chamber longitudal section is 1.3 times greater than object droplet (or cell) diameter and is less than 2 times of object droplet (or cell) diameter for best (1.3 < d < 2).Micro-fluidic chip provided by the utility model can be used for water phase and oily phase system, capture single aqueous phase droplets (or single drop of cladding mono-/multi- cell), Yu Shuixiang system acquisition single cell, microorganism in oily phase system.
Description
Technical field
The utility model relates to micro-fluidic fields, the in particular to device of micro-fluidic chip and capture simple target object.
Background technique
Microfluidic chip technology (Microfluidics) is otherwise known as chip lab (Lab-on-a-chip), can be one
The basic function in traditional biological and chemical laboratory, including sample separation, system are integrated on a several square centimeters of micro chip
The operations such as standby, chemical reaction, detection.
Controllable, consumption sample and reagent are few with liquid flowing for micro-fluidic chip, with analyzing speed tenfold hundreds of times
The features such as raising, analysis while it can carry out a sample up to a hundred within a few minutes even shorter time, and can be
Line realizes the pretreatment and analysis overall process of sample.Drop microflow control technique is an important branch of microfluidic chip technology.
Drop microflow control technique is developed in traditional single-phase microfluidic chip technology, earliest by Chicago University Rustem
Professor F.Ismagilov proposes that three entrance T-type microlayer model chips design first, and obtain in several years later extensive concern and
Using.Compared with single-phase microfluidic system, due to its water/oil two-phase laminated flow feature, have such as consumption sample and amount of reagent more
Few, mixing velocity does not easily cause cross contamination faster, the advantages such as is easily manipulated.Therefore, it is detected in pollutant fast high-flux, it is raw
The separation of object sample is cultivated, and observation extent of chemical reaction etc. has important application in fields.Microlayer model is because having flux height, nothing
The advantages such as cross contamination show huge in fields such as inkjet printing, microring array, DNA analysis, materials synthesis, crystallization of protein
Big application potential.
Cell, bacterium, microorganism observation and image analysing computer during, usually all using group as object observing, therefore
Such observation is all summarized generally, if there is special single variation, has no way of realizing at all, therefore can be to cell, thin
It is currently to need urgent problem to be solved that bacterium, microorganism, which carry out individually observation and image analysing computer,.
Utility model content
In view of this, the present invention provides a kind of micro-fluidic chip and the devices of capture simple target object.The chip can
Independent part is captured, conveniently reaches and the demands such as image analysing computer, fluorescence reaction analysis is carried out with microexamination.
In order to realize above-mentioned purpose of utility model, the utility model the following technical schemes are provided:
The utility model provides a kind of micro-fluidic chip, including substrate 1, and the substrate 1 is provided with injection port 4, goes out sample
Mouth 5, fluid channel 2 and shape for hat chamber 3；
The shape for hat chamber 3 is set between injection port 4, outlet 5, and the opening of the shape for hat chamber 3 with it is described micro-
Runner 2 is connected to.
Preferably, the Opening length of 3 longitudal section of shape for hat chamber is not less than object droplet and/or the diameter of cell.
It is highly preferred that the ratio of the diameter of the Opening length of 3 longitudal section of shape for hat chamber and object droplet and/or cell is not
Less than 1.3:1.
It is highly preferred that the Opening length of 3 longitudal section of shape for hat chamber and the ratio of the diameter of object droplet and/or cell are
1.3~2:1.
Preferably, the longitudal section of the shape for hat chamber 3 is rectangle, arch or trapezoidal.
Preferably, the number of the shape for hat chamber 3 is no less than 1.
Based on the above-mentioned technical proposal, the utility model additionally provides the micro-fluidic chip in capture simple target object
Using；The object includes drop, cell, microorganism.
Based on the above-mentioned technical proposal, the utility model additionally provides a kind of device for capturing simple target object, including described
Micro-fluidic chip；The object includes drop, cell, microorganism.
Based on the above-mentioned technical proposal, the utility model additionally provides a kind of method for capturing simple target object, to described micro-
It is passed through sample, water phase and/or oily phase in fluidic chip, adjusts the density of the water phase and/or oily phase, makes the simple target object
Into the shape for hat chamber 3；The object includes drop, cell, microorganism.
Preferably, further including the overturning micro-fluidic core after single drop and/or cell enter the shape for hat chamber 3
Piece makes the simple target object leave the shape for hat chamber 3, into the fluid channel 2, the step of collection.
Micro-fluidic chip provided by the utility model has special shape for hat chamber structure, and each chamber is all small detection
Analysis room, the length of chamber longitudal section are greater than object droplet (or cell), and the length of chamber longitudal section is greater than object droplet
1.3 times of (or cell) diameter and it is less than 2 times of object droplet (or cell) diameter for best (1.3 < d < 2).The utility model
The micro-fluidic chip of offer can be used for water phase and oily phase system, capture single aqueous phase droplets (or cladding mono-/multi- cell in oily phase system
Single drop), Yu Shuixiang system acquisition single cell, microorganism.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described.
Fig. 1 shows micro-fluidic chip provided by the utility model；Wherein Figure 1A shows top view；Figure 1B shows side view；
Fig. 2 shows micro-fluidic chip provided by the utility model for capturing single drop schematic diagram；Wherein, Fig. 2A shows this
The micro-fluidic chip that utility model provides captures single drop；Fig. 2 B shows in shape for hat chamber with microscope observation analysis；Fig. 2 C
Show and reverse micro-fluidic chip provided by the utility model, so that drop enters fluid channel from shape for hat chamber to receive in outlet
Collection；
Fig. 3 shows single drop and is coated with the result that single celled single drop is captured by shape for hat chamber；
When Fig. 4 shows that droplet density is greater than solution density, using the reverse geometry of Fig. 1, single drop schematic diagram is captured；
Fig. 5 shows the density of adjustment solution, makes the density of solution between object and non-targeted object to be separated, thus
Carry out different cell separation；
Wherein, 1- substrate；2- fluid channel；3- shape for hat chamber；4- injection port；5- outlet.
Specific embodiment
The utility model discloses a kind of micro-fluidic chip and the devices of capture simple target object, and those skilled in the art can
To use for reference present disclosure, it is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to this
It is it will be apparent that they are considered as being included in the utility model for the technical staff of field.The method of the utility model and
Using being described by preferred embodiment, related personnel obviously can not depart from the content of the present invention, spirit and
To method described herein and application is modified or appropriate changes and combinations in range, carry out implementation and application the utility model skill
Art.
Used unit, raw material and reagent in the device of micro-fluidic chip provided by the utility model and capture simple target object
It is available on the market.
The utility model provides a kind of micro-fluidic chip, including substrate 1, and the substrate 1 is provided with injection port 4, goes out sample
Mouth 5, fluid channel 2 and shape for hat chamber 3；
The shape for hat chamber 3 is set between injection port 4, outlet 5, and the opening of the shape for hat chamber 3 with it is described micro-
Runner 2 is connected to.
In some specific embodiments of the utility model, the Opening length of 3 longitudal section of shape for hat chamber is not less than target
The diameter of drop and/or cell.Preferably, the diameter of the Opening length of 3 longitudal section of shape for hat chamber and object droplet and/or cell
Ratio be not less than 1.3:1.It is highly preferred that the diameter of the Opening length of 3 longitudal section of shape for hat chamber and object droplet and/or cell
Than for 1.3~2:1.
In some specific embodiments of the utility model, the longitudal section of shape for hat chamber 3 is rectangle, arch or ladder
Shape.
In some specific embodiments of the utility model, the number of the shape for hat chamber 3 is no less than 1.
Because the diameter of analysis object is different (such as: bacterium, cell, microorganism), or the diameter of drop generated is not
Together, the length of shape for hat chamber longitudal section can also change correspondingly (1.3 < d < 2), can answer the capture of various different target objects.Change cap
The size of type chamber, the shape of longitudal section, the increase and decrease of the quantity of shape for hat chamber are easy to accomplish for those skilled in the art,
This will not be repeated here for the utility model, the length of shape for hat chamber longitudal section of all suitable objects, the structure of shape for hat chamber and
Quantity both is within the protection scope of the present invention.
Based on the above-mentioned technical proposal, the utility model additionally provides the micro-fluidic chip in capture simple target object
Using；The object includes drop, cell, microorganism.
Based on the above-mentioned technical proposal, the utility model additionally provides a kind of device for capturing simple target object, including described
Micro-fluidic chip；The object includes drop, cell, microorganism.
Based on the above-mentioned technical proposal, the utility model additionally provides a kind of method for capturing simple target object, to described micro-
It is passed through sample, water phase and/or oily phase in fluidic chip, adjusts the density of the water phase and/or oily phase, makes the simple target object
Into shape for hat chamber 3；Object includes drop, cell, microorganism.In other specific embodiments of the utility model,
Further include overturning the micro-fluidic chip after single drop and/or cell enter shape for hat chamber 3, make the simple target object from
Shape for hat chamber 3 is opened, into fluid channel 2, the step of collection.
As shown in Figure 1, micro-fluidic chip provided by the utility model by the structure of shape for hat chamber and the density of grease not
It, to reach the target that single sample droplets can only enter single shape for hat chamber, is in structure design general with cap with collocation mutually
It reads, several bulge-structures (cavity) is set on the surface of fluid channel, and these bulge-structures are then catching for each object
Obtain observation analysis chamber.Shape for hat chamber 3 can be seen by the side view of Fig. 1 to connect with fluid channel 2, it will be because of oil when sample enters
It is different from the density of water that (entering in shape for hat chamber 3 (Fig. 2A) when drop flows through shape for hat chamber 3 can be thin to microscopically observation
Born of the same parents' type, fluorescence analysis, immuning fluorescent dyeing analysis etc. (Fig. 2 B).Or with assisted automated point of automatic fluorescence analysis pertinent instruments
Analysis, and observe with analysis terminate also can be taken off sample with benefit it is subsequent it is further react, analysis or culture use or further under
Micro-fluidic chip overturning drop can be left shape for hat chamber 3, then arrange in pairs or groups and test buffer solution and take it out by trip analysis
(Fig. 2 C).
Micro-fluidic chip provided by the utility model is in the case where shape for hat chamber structure is different from the density of grease (with this
For example, that is, water than oil it is light), make drop can flowing when simultaneously float and stop capture into shape for hat chamber.If the density of drop
Greater than oily phase, the reverse geometry of Fig. 1 can be used to be captured, make drop when flowing while sinking and being trapped in Micro-v oid.
If the density of drop is greater than oily phase, Fig. 4 structure can be used to be captured.
When detection and analysis, which terminate, to take out object, only need to overturn micro-fluidic chip be can be taken off.It can be according to water phase
Or the density of oily phase is different, progress selectivity capture object (coats in unicellular, single drop, single microbial, or single drop
Unicellular/many cells).
It, can also size be each other according to different target object in addition, on the basis of micro-fluidic chip provided by the utility model
Together, selective capture is carried out, the microparticle (or biomone) that will be greater than Micro-v oid filters out, and only capture is less than the micro- of Micro-v oid
Particle (or biomone).
Micro-fluidic chip provided by the utility model combines the density of adjustment water phase and/or oily phase, reaches the single mesh of capture
Mark the purpose of object.
In water phase, the density of water phase is adjusted, so that cell is floated to superjacent (the density ratio solution of cell is small), whereby
The characteristic of solution makes cell float into shape for hat chamber in conjunction with the shape for hat chamber structure of micro-fluidic chip provided by the utility model
It is observed.
On this basis, the solution of adjustable different densities, separates object.According to the volume of different cells
Difference, density difference adjust the density of solution, make the density of solution between object and non-targeted object to be separated, from
And carry out different cell separation.Such as red blood cell and white blood cell, red blood cell and circulating tumor cell etc..According to the base of different cells
The different density to adjust solution of plinth characteristic (volume different, density different) make non-targeted object can not be by solution influenced (or past
Sink), and non-targeted object is made to be floated to shape for hat chamber because of solution, achieve the effect that separation (Fig. 5) whereby.
In oily phase, the density of oily phase is adjusted between object to be separated the solution of different densities (or containing the particle of solution)
Between other non-targeted objects (particles of cladding different densities in the solution or same solution of different densities), reach different densities
Liquid (microlayer model) separation, or in the identical micro- liquid of separation, coat the selection of the particle (or biological particle) of different densities
Property capture.Micro-fluidic chip provided by the utility model can be applied in magnetic bead immunocapture cell system, exempt from specific cells
The magnetic bead group and do not captured with the selective filter of the single small magnetic bead of cell combination and/or selectivity that epidemic disease combines.
The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art
For art personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these improve and
Retouching also should be regarded as the protection scope of the utility model.
Claims (7)
1. micro-fluidic chip, which is characterized in that including substrate (1), the substrate is provided with injection port (4), outlet (5), micro-
Runner (2) and shape for hat chamber (3)；
The shape for hat chamber (3) is set between injection port (4), outlet (5), and the opening of the shape for hat chamber (3) and institute
State fluid channel (2) connection.
2. micro-fluidic chip according to claim 1, which is characterized in that the opening of shape for hat chamber (3) longitudal section is long
Degree is not less than object droplet and/or the diameter of cell.
3. micro-fluidic chip according to claim 2, which is characterized in that the opening of shape for hat chamber (3) longitudal section is long
Degree is not less than 1.3:1 with the ratio of object droplet and/or the diameter of cell.
4. micro-fluidic chip according to claim 3, which is characterized in that the opening of shape for hat chamber (3) longitudal section is long
Degree is (1.3~2) with the ratio of object droplet and/or the diameter of cell: 1.
5. micro-fluidic chip according to claim 4, which is characterized in that the longitudal section of the shape for hat chamber (3) is rectangular
Shape, arch or trapezoidal.
6. micro-fluidic chip according to claim 5, which is characterized in that the number of the shape for hat chamber (3) is no less than 1
It is a.
7. a kind of device for capturing simple target object, which is characterized in that including as claimed in any one of claims 1 to 6 micro-fluidic
Chip；The object includes drop, cell, microorganism.
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| CN201821562851.5U CN209636230U (en) | 2018-09-20 | 2018-09-20 | Micro-fluidic chip and the device for capturing simple target object |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113318799A (en) * | 2021-06-30 | 2021-08-31 | 上海天马微电子有限公司 | Microfluidic device, manufacturing method thereof and microbead screening method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113318799A (en) * | 2021-06-30 | 2021-08-31 | 上海天马微电子有限公司 | Microfluidic device, manufacturing method thereof and microbead screening method |
| CN113318799B (en) * | 2021-06-30 | 2023-12-29 | 上海天马微电子有限公司 | Microfluidic device, manufacturing method thereof and microbead screening method |
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| TR01 | Transfer of patent right |
Effective date of registration: 20230629 Address after: Room 101, No. 37, Lane 1566, Guoquan North Road, Yangpu District, Shanghai, 200082 Patentee after: Shanghai Xingesai Biotechnology Co.,Ltd. Address before: 100093 no.301-9, 3rd floor, building 2, zone B, central liquid cooling and heating source environmental system industrial base project, No.80 xingshikou Road, Haidian District, Beijing Patentee before: BEIJING YITIAN JIARUI TECHNOLOGY Co.,Ltd. |
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