CN207567248U - For extracting the reagent handling device of flesh tissue nucleic acid - Google Patents

Abstract

Including container, centrifuge tube, adsorption column and dropper are mounted in container respectively by card slot or lattice gear for a kind of reagent handling device for being used to extract flesh tissue nucleic acid.The device of the utility model can be carried out in the step of extracting supernatant or lower turbid by extraction tube.The height of the extraction of extraction tube is arranged on the liquid level for needing to extract and can effectively avoid accidentally extraction up and down.The relevant operation of adsorption column can be carried out directly by the opening in support construction, easy to operate.Can constant temperature protection be carried out by pipe sleeve in centrifugally operated, sample qualities is avoided to deteriorate.Extraction to medicament can rely on the scale control medicament extracted amount on dropper.After extraction, the RNA samples that do not use at once can also directly be sealed refrigeration guaranteed quality by container, avoid being transferred to sample decomposed metamorphic in freezen protective way.Due to the cooperation of above device, the loss during the utility model RNA material extractions can significantly be improved, and extraction operation is more easy.

Description

For extracting the reagent handling device of flesh tissue nucleic acid

Technical field

The utility model is related to a kind of tissue extraction device more particularly to a kind of examinations for being used to extract flesh tissue nucleic acid Agent processing unit.

Background technology

In general, the RNA extractions of different samples have different operation requirements.Existing kit, function is single, usually only Sample, reagent can be accommodated, the operations demand in extraction can not be met.For hydrothorax sample, it usually needs will be collected Individual sample centrifuged, precipitated, being shaken, is homogenized and etc. pretreatment.After pretreatment, also need to add in again reagent carry out from The operations such as the heart, absorption are so that nucleic acid is detached and stablized.It is complex for operation step, and it is various to be related to reagent, instrument type.

Biological nucleic acid marker as target analytes, needs to repeat to test in the diagnosis of specified disease.Cause How this, improve testing efficiency, how to reduce test condition requirement, reduces cost, be the design key institute of nucleic acid test device .

However, due to the sensibility of RNA materials, sample handling processes need to protect the complete of these unstable molecules Property.Especially for hydrothorax sample etc., how to ensure that RNA is not destroyed in sample, extracted so as to fulfill effective RNA, be current One of break-through point of nucleic acid test device.

At present, it is simple in structure to be badly in need of one kind, can adapt to every processing in sample rna extraction process, and easy to operate RNA materials device.

Invention content

In order to solve the shortcomings of the prior art, the purpose of this utility model is to provide one kind for extracting fresh group The reagent handling device of nucleic acid is knitted, the apparatus structure is simple, is applicable in the RNA extraction process steps of common flesh tissue sample, And RNA material losses are less, and extraction operation is more easy.

It is to achieve the above object, provided by the utility model for extracting the reagent handling device of flesh tissue nucleic acid, Include at least one card slot including container, in the container or lattice kept off, the card slot or lattice gear be mounted with respectively centrifuge tube, Adsorption column and dropper, which is characterized in that for the dropper by dropper mouth to operation portion, sequence identification has volume scale；

Pipe sleeve is also arranged in the card slot or lattice gear, which is characterized in that the internal diameter of the pipe sleeve and the centrifuge tube Outer diameter matches；

The outer wall of the container or the pipe sleeve is double-layer structure, and the double-layer structure is sandwiched between temperature-reducing medium.

Further, in above device, extraction tube is also separably arranged in the centrifuge tube；

The extraction tube is hollow, and, the extraction tube is also set on the side wall apart from the centrifugation bottom of the tube height of the extraction There is perforation.

Further, in above device, the downside of the perforation on the outside of the extraction tube, is additionally provided with support construction.

Specifically, deviate in the support construction at the position that the support construction of above device is connect with the extraction tube The heart.The structures such as the supporting rod for the setting that intersects, Eccentric Circular Ring can be used in support construction.

Preferably, in above device, the vertical or horizontal size of support construction matches with the centrifuge tube inner diameter It closes, and the support construction surface is equipped at least one opening.

Further, for convenience of RNA samples are directly extracted after pre-processing, in above device, the opening size is large enough to hold The adsorbed film of the absorption column end.

And to avoid pollution sample, in above device, the extraction tube and the support construction are medical silica-gel Material or medical grade rubber material.

The temperature-reducing medium includes water, ice or its mixing or other have the thermostabilization solvent of big specific heat coefficient.

The utility model is compared with existing scheme and is had the following technical effect that:

1. the device of the utility model can be carried out in the step of extracting supernatant or lower turbid by extraction tube.Match Ground is closed, the height of the extraction of extraction tube is arranged on above and below the liquid level for needing to extract by support construction, so as to effectively avoid accidentally carrying It takes.The relevant operation of adsorption column can be carried out directly by the opening in support construction, easy to operate.It can be by pipe sleeve in centrifugally operated Constant temperature protection is carried out, sample qualities is avoided to deteriorate.Extraction to medicament can rely on the scale control medicament extracted amount on dropper. After extraction, the RNA samples that do not use at once can also directly be sealed refrigeration guaranteed quality by container, avoid being transferred to freezing Preserve sample decomposed metamorphic in way.Due to the cooperation of above device, the loss during the utility model RNA material extractions can be bright Seem to improvement, extraction operation is more easy；

2. further, the device of the utility model can avoid dirt using silica gel or medical grade rubber material, stability higher Contaminate sample.Simultaneously as the elasticity of material in itself, adsorption column relevant operation can directly pass through adsorption column in the support construction Opening carry out.Since extraction tube integral material elastic range is big, can directly be compressed by adsorption column, therefore will not shadow Ring adsorption operations.It is convenient directly to extract or carry out subsequent operation to sample.

Flesh tissue sample progress RNA is carried 3. above-mentioned improved structure with an entirety is synthesized, is suitable for the overwhelming majority The operation taken.The synthesis utilization rate of device is higher, and the mutual cooperation relationship between each structure can effectively improve RNA extraction effects Rate.

Other features and advantages of the utility model will illustrate, also, in the following description partly from specification In become apparent or by implement the present invention and understand.

Description of the drawings

Attached drawing is used to provide a further understanding of the present invention, and a part for constitution instruction, and with this reality With novel embodiment together, for explaining the utility model, the limitation to the utility model is not formed.In the accompanying drawings：

Fig. 1 is the reagent handling device structure diagram for being used to extract flesh tissue nucleic acid according to the utility model.

Specific embodiment

The preferred embodiment of the utility model is illustrated below in conjunction with attached drawing, it should be understood that described herein excellent Embodiment is selected to be only used for describing and explaining the present invention, is not used to limit the utility model.

Fig. 1 is the reagent handling device structure diagram for being used to extract flesh tissue nucleic acid according to the utility model, As shown in Figure 1, the reagent handling device for being used to extract flesh tissue nucleic acid of the utility model, including container 1, the appearance It puts and includes at least one card slot or lattice gear in box, the card slot or lattice gear are mounted with centrifuge tube 2, adsorption column 3 and dropper 4 respectively, It is characterized in that, the dropper 4, by dropper mouth to operation portion, sequence identification has volume scale；

Pipe sleeve 5 is also arranged in the card slot or lattice gear, which is characterized in that the internal diameter of the pipe sleeve and the centrifuge tube 2 Outer diameter match, fit with the outer wall construction of the centrifuge tube；

The outer wall of the container 1 or the pipe sleeve 5 is double-layer structure, and the double-layer structure is sandwiched between temperature-reducing medium 51.Wherein, common structure can be used in adsorption column 3, including upper cover, adsorbed film and tube body.

Further, in above device, extraction tube 21 is also separably arranged in the centrifuge tube；

The extraction tube 21 is hollow, and, on side wall of the extraction tube 21 apart from the centrifugation bottom of the tube height of the extraction It is additionally provided with perforation 22.

Further, in above device, the downside of the perforation 22 around 21 outside of extraction tube, is additionally provided with support Structure 23.

Specifically, deviate the support construction in the position that the support construction 23 of above device is connect with the extraction tube 21 23 center.The structures such as the supporting rod for the setting that intersects, Eccentric Circular Ring can be used in support construction 23.

Preferably, in above device, the 23 vertical or horizontal size of support construction and the centrifuge tube inner diameter phase Cooperation, and 23 surface of the support construction is equipped at least one opening 24.

Further, for convenience of RNA samples are directly extracted after pre-processing, in above device, 24 sizes of the opening are held enough Receive it is described absorption column end adsorbed film.

And to avoid pollution sample, in above device, the extraction tube 21 and the support construction 23 are medical Silica gel material or medical grade rubber material.

The temperature-reducing medium 51 includes water, ice or its mixing or other have the thermostabilization solvent of big specific heat coefficient.

Below for extracting hydrothorax sample rna, the course of work of this system is illustrated：

First, it takes in the centrifuge tube 2 in 10 to 40mL hydrothorax sample to container 1,300 × g centrifugations 10min.From During the heart, to ensure sample quality, pipe sleeve 5 can be sheathed on outside the centrifuge tube 2, carry out centrifugally operated together.It will extraction Pipe 21 is placed in the centrifuge tube 2.Extraction tube 21 keeps perforation 22 and centrifugation bottom of the tube in it by the support construction 23 on the outside of it Distance, supernatant is removed by perforation 22 on the extraction tube side wall.Here, the extraction tube 21 is apart from the centrifuge tube bottom The height of the extraction in portion can set concordant or slightly higher with the liquid level of lower turbid.After removing supernatant, it can will be precipitated by dropper 4 It is transferred in 1.5mL centrifuge tubes, by clean dropper 4, reads its high scale, extraction 300 μ LBufferTC injection precipitations are shaken It swings uniformly, is inserted in pipe sleeve 5 one and is placed in centrifuge, 300 × g centrifugations 10min.According to aforesaid operations supernatant is removed again, then add Enter 700 μ LBufferRFB homogenizeds, extracted in 600 μ L tissue homogenate to 1.5mL centrifuge tubes by clean dropper.

Then, above-mentioned pretreatment sample is subjected to RNA separating steps.Step mainly includes：

Add the ProteinaseKSolution of 20 μ L, piping and druming is uniform, 56 DEG C of digestion 15min；Hand held centrifuges 5 ~ 10s；

Add 320 μ L absolute ethyl alcohols, piping and druming is uniform, and hand held centrifuges 5 ~ 10s；

600 μ L liquid are extracted to RNA adsorption columns, 12000 × g centrifugation 30s are removed and collected liquid in pipe；

By remaining liq, all extraction to RNA adsorption columns, 12000 × g centrifugation 30s is removed and collects liquid in pipe；

600 μ L Buffer RTW are extracted to RNA adsorption columns, 12000 × g centrifuges 30 s, removes liquid in collecting pipe Body；

Continue to extract in 600 μ L Buffer RTW to RNA adsorption columns, 12000 × g centrifuges 30 s, removes in collecting pipe Liquid；

The collecting pipe more renewed, 12000 × g centrifuge 5 min, abandon collecting pipe；

Adsorption column is carefully transferred in 1.5 clean mL centrifuge tubes, 30 ~ 80 μ L are added dropwise toward RNA absorption center membranes Buffer RTE, are stored at room temperature 3 min, and 12000 × g centrifugation 1min collect sample RNA and preserve；

The RNA samples of extraction are positioned over Cord blood in container, are not used more than 2 h, by container by sample It is transferred in less than -70 DEG C environment and preserves.

Wherein, the step of similar above-mentioned pretreatment operation, extraction supernatant or lower turbid, can be carried out by extraction tube 21.It carries The height of the extraction of pipe 21 is taken to be set to 2 diameter of centrifuge tube to match, above and below the liquid level for needing to extract.3 relevant operation of adsorption column Directly the opening 24 in the support construction 23 can be passed through to carry out adsorption column.Elastic material is used since extraction tube 21 is whole （Medical silica-gel or rubber）It can be compressed by adsorption column, therefore do not interfere with adsorption operations.Centrifugally operated can be by pipe sleeve 5 Constant temperature protection is carried out, sample qualities is avoided to deteriorate.Extraction to medicament can be carried out by dropper 4.Because there is label on dropper Degree, therefore the measurement of medicament extracted amount is more convenient, accurate.After extraction, the RNA samples that do not use at once can be straight by container 1 Sealing refrigeration guaranteed quality is connect, avoids sample decomposed metamorphic in the way of -70 DEG C of freezen protectives of transfer.Due to matching for above device It closes, the loss during the utility model RNA material extractions can significantly be improved, and extraction operation is more easy.

For convenience of being cleaned to dropper, it can also pass through card slot or lattice gear placement cleaning solution in container 1.For convenience of processing Waste liquid, it is similar, waste collection bottle can be also added in container, Biohazard Waste is handled to concentrate.

One of ordinary skill in the art will appreciate that：The above descriptions are merely preferred embodiments of the present invention, and Limitation the utility model is not used in, although the utility model is described in detail with reference to the foregoing embodiments, for ability For the technical staff in domain, the technical solution that can be still recorded to foregoing embodiments modifies or to its middle part Technical characteristic is divided to carry out equivalent replacement.Within the spirit and principle of the utility model, any modification for being made equally is replaced It changes, improve, should be included within the scope of protection of this utility model.

Claims (8)

1. it is a kind of for extracting the reagent handling device of flesh tissue nucleic acid, including container（1）, the container is interior to be wrapped At least one card slot or lattice gear are included, the card slot or lattice gear are mounted with centrifuge tube respectively（2）, adsorption column（3）And dropper（4）, It is characterized in that, the dropper（4）By dropper mouth to operation portion, sequence identification has volume scale；

Pipe sleeve is also arranged in the card slot or lattice gear（5）, which is characterized in that the internal diameter of the pipe sleeve and the centrifuge tube（2） Outer diameter match；

The container（1）Or the pipe sleeve（5）Outer wall for double-layer structure, the double-layer structure is sandwiched between temperature-reducing medium （51）.

It is 2. as described in claim 1 for extracting the reagent handling device of flesh tissue nucleic acid, which is characterized in that it is described from Extraction tube is also separably arranged in heart pipe（21）；

The extraction tube（21）To be hollow, and, the extraction tube（21）On side wall apart from the centrifugation bottom of the tube height of the extraction It is additionally provided with perforation（22）.

3. the reagent handling device as claimed in claim 2 for being used to extract flesh tissue nucleic acid, which is characterized in that described to wear Hole（22）Downside, around the extraction tube（21）Outside is additionally provided with support construction（23）.

4. the reagent handling device as claimed in claim 3 for being used to extract flesh tissue nucleic acid, which is characterized in that the branch Support structure（23）With the extraction tube（21）Deviate the support construction in the position of connection（23）Center.

5. the reagent handling device as claimed in claim 4 for being used to extract flesh tissue nucleic acid, which is characterized in that the branch Support structure（23）Vertical or horizontal size is matched with the centrifuge tube inner diameter, and the support construction（23）Surface is equipped with At least one opening（24）.

6. the reagent handling device as claimed in claim 5 for being used to extract flesh tissue nucleic acid, which is characterized in that described to open Mouthful（24）Size is large enough to hold the adsorbed film of the absorption column end.

7. the reagent handling device for being used to extract flesh tissue nucleic acid as described in claim 3 to 6 is any, feature exist In the extraction tube（21）And the support construction（23）It is medical silica-gel material or medical grade rubber material.

8. the reagent handling device as described in claim 1 for being used to extract flesh tissue nucleic acid, which is characterized in that the drop Warm medium（51）Including water, ice or its mixing.