CN207567247U - At the uniform velocity change liquid and the suspension cell combination culture apparatus of dosing - Google Patents
At the uniform velocity change liquid and the suspension cell combination culture apparatus of dosing Download PDFInfo
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- CN207567247U CN207567247U CN201721691185.0U CN201721691185U CN207567247U CN 207567247 U CN207567247 U CN 207567247U CN 201721691185 U CN201721691185 U CN 201721691185U CN 207567247 U CN207567247 U CN 207567247U
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- liquid
- culture bottle
- dosing
- changing device
- suspension cell
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Abstract
The utility model discloses a kind of suspension cells at the uniform velocity changing liquid and dosing to combine culture apparatus, including culture bottle, the first liquid changing device, the second liquid changing device and a drug vial；First liquid changing device changes liquid pipe by first and is connected with the culture bottle；Second liquid changing device changes liquid pipe by second and is connected with the culture bottle；Second changes in liquid pipe and sets there are one lateral, and the drug vial is connected on the lateral, and then is connected with the culture bottle.The utility model contributes to experimenter to carry out at the uniform velocity change to suspension cell, controllableization changes liquid and ensures that suspension cell changes the sterilization of liquid process；Experimenter is contributed to carry out at the uniform velocity change, controllableization dosing to suspension cell and ensures to be sterile filtered to drug added by suspension cell, and contributes to routine culture suspension cell and ensures suspension cell always in suspension cultivation conditions, reasonable aeration status.
Description
Technical field
The utility model is related to cell culture apparatus technical fields, and in particular to a kind of at the uniform velocity to change liquid and the suspension of dosing is thin
Born of the same parents combine culture apparatus.
Background technology
Suspension cell culture (suspension cell culture) is an extremely complex cell culture technology, this
Technology will not only keep suspension cell to be in suspended state, while also keep germ-free condition, once in suspension cell culture mistake
Occur the pollutions such as bacterium, virus, mycoplasma, fungi in journey, just influence whether suspension cell culture, be subsequent experimental study band
Carry out unnecessary interference.
In previous suspended cell culture technic, suspending cell culture bottle bottle cap needs repeatedly to open and still cannot be real
Now to suspension cell at the uniform velocity change, controllably change change liquid, dosing；For this reason, it is necessary to develop novel suspension cell culture dress
It puts.
Invention content
For the defects in the prior art, the purpose of this utility model is to provide one kind can be convenient for suspension cell into
Row changes liquid and dosing, and what can rationally be ventilated at the uniform velocity changes liquid and the suspension cell combination culture apparatus of dosing.
To achieve the above object, the utility model uses following technical scheme：
A kind of suspension cell combination culture apparatus at the uniform velocity changing liquid and dosing, which is characterized in that including：Culture bottle, first
Liquid changing device, the second liquid changing device and a drug vial；First liquid changing device changes liquid pipe by first and is connected with the culture bottle；Institute
It states the second liquid changing device and changes liquid pipe by second and be connected with the culture bottle；Second changes in liquid pipe and sets there are one lateral, described
Drug vial is connected on the lateral, and then is connected with the culture bottle.
Preferably, further include a uniform rotation device and an incubator；The culture bottle be installed in it is described at the uniform velocity
Magnetic force at the uniform velocity rotor is placed on tumbler, in culture bottle；The uniform rotation device is placed on the bottom plate of incubator, is added
Medicine bottle is suspended on the top plate of incubator.
Preferably, the drug vial is connected to by a chemical feed pipe on the lateral；It is set on the chemical feed pipe
There are dosing buffering area and dosing tube valve.
Preferably, described second changes liquid pipe and is changed equipped with second and liquid pipe valve and change liquid pipe filter；The branched pipe
Road is connected to described second and changes liquid pipe valve and change between liquid pipe filter；Described change in liquid pipe filter is equipped with one layer of mixing fibre
Tie up film；Described first changes liquid pipe changes liquid pipe valve equipped with first.
Preferably, the culture bottle is equipped with culture bottle cap；Described first, which changes liquid pipe and second, changes liquid pipe across institute
Culture bottle cap is stated with culture bottle to be connected；Described first changes liquid pipe deeply to culture bottle bottom.
Preferably, the culture bottle has the plated film culture bottle of polymethyl methacrylate film for inner surface, in
Culture bottle is distributed in equipped with one layer and cultivates the silicone hydroxyl film of bottle cap inner surface.
Preferably, being set on the culture bottle cap, there are one bottle cap air filters；It is equipped in the air filter
One layer of 0.45 μm of bore filter film and one layer of 0.22 μm of bore filter film.
Preferably, first liquid changing device has the first liquid changing device piston and first manual gear；The first manual
Gear can drive the rack on the first liquid changing device piston to move, and liquid purpose is changed so as to reach；The second liquid changing device tool
There are the second liquid changing device piston and the second manual gear；Second manual gear can be driven on the second liquid changing device piston
Rack moves, and liquid purpose is changed so as to reach.
Compared with prior art, the beneficial benefit of the utility model is：The utility model contributes to experimenter to suspending
Cell carries out at the uniform velocity change, controllableization and changes liquid and ensure that suspension cell changes the sterilization of liquid process；Contribute to experimenter to suspending
Cell carries out at the uniform velocity change, controllableization dosing and ensures to be sterile filtered to drug added by suspension cell, is more conducive to conventional training
It supports suspension cell and ensures suspension cell always in suspension cultivation conditions, reasonable aeration status, final the utility model can be with
Realize the at the uniform velocity change that liquid process and dosing process are changed suspension cell, controllableization, sterilization.
Description of the drawings
Fig. 1 is the structure diagram of the utility model.
Fig. 2 is the structure diagram of plated film culture bottle cap.
Fig. 3 is the structure diagram for changing liquid pipe filter.
Fig. 4 is the structure diagram of bottle cap air filter.
Specific embodiment
As shown in Figure 1, the utility model discloses a kind of suspension cell combination culture dresses at the uniform velocity changing liquid and at the uniform velocity dosing
Put, including plated film culture bottle 45, first change liquid pipe 24, second change liquid pipe 26, bottle cap air filter 25, the first liquid changing device 2,
Second liquid changing device 12, chemical feed pipe 38 and drug vial 34.Wherein plated film culture bottle 45 by polyethylene terephthalate prepare and
Into maximum safe volume is 300mL；Plated film culture bottle 45 be equipped with plated film culture bottle cap 44, plated film culture bottle cap 44 with
Plated film culture bottle 45 is connected through a screw thread；Plated film culture bottle 45 is positioned on uniform rotation device 41 and uniform rotation device 41 is put
It is placed on incubator bottom plate 43 and (does not draw incubator in figure)；Magnetic force at the uniform velocity rotor 42 is placed in plated film culture bottle 45；Magnetic force
At the uniform velocity a diameter of 4mm of rotor 42, length 10mm.The rotation driving force of magnetic force at the uniform velocity rotor 42 comes from uniform rotation device
41, the uniform rotation speed of uniform rotation device 41 is 35 turns/min.
First one end for changing liquid pipe 24 is inserted into 45 bottom of bottle region of plated film culture bottle and first other end for changing liquid pipe 24 passes through
Quick coupling 21 is directly connected to the first liquid changing device 2, and first changes liquid pipe 24 equipped with having hand on manually-operated gate 23 and manually-operated gate 23
Movable valve knob 22.Second changes liquid pipe 26 is inserted into plated film culture bottle cap 44 for F character form structures and second one end for changing liquid pipe 26
Second change in liquid pipe latex hole 49, the second other both ends for changing liquid pipe 26 are connected respectively by quick coupling 48, quick coupling 30
Connect the second liquid changing device 12, chemical feed pipe 38.Second changes liquid pipe 26 equipped with manually-operated gate 29, and manually-operated gate 29 is equipped with manually-operated gate
Knob 28.
45 inner surface of plated film culture bottle is polymethyl methacrylate film, and the thickness of polymethyl methacrylate film is
0.3mm, the inner surface of polymethyl methacrylate film are that the thickness of silicone hydroxyl film 46 and silicone hydroxyl film 46 is 0.6mm,
Silicone hydroxyl film 46 is distributed in the inner surface of 45 inner vessel surface of plated film culture bottle and plated film culture bottle cap 44.
As shown in Fig. 2, there is first to change liquid pipe latex hole 51, bottle cap air filter latex hole on plated film culture bottle cap 44
50th, second liquid pipe latex hole 49 is changed；First changes liquid pipe latex hole 51, liquid pipe breast is changed in bottle cap air filter latex hole 50, second
Traveling first changes liquid pipe 24, bottle cap air filter 25, second changes liquid pipe 26 respectively in glue hole 49.
As shown in figure 3, second change liquid pipe 26 be equipped with change liquid pipe filter 27；It changes in liquid pipe filter 27 equipped with one layer small
Bore filter film 54 (the composite fibre film of 0.22 μm of pore diameter).
As shown in figure 4, bottle cap air filter 25 is has one layer of large aperture in T-shaped structure and bottle cap air filter 25
Filter membrane 52 and one layer of small-bore filter membrane 53, composite fibre film and aperture of the large aperture filter membrane 52 for 0.45 μm of pore diameter
Composite fibre film of the diameter filter membrane 53 for 0.22 μm of pore diameter.
The safe volume of maximum of first liquid changing device 2 is 50mL, has and changes the liquid changing device that liquid pipe 24 is connected with first and export
1；First liquid changing device, 2 outer surface is equipped with liquid changing device scale value 3, is internally provided with the first liquid changing device piston 4, and tail portion is equipped with metal shaft
Fixed plate 10；First liquid changing device piston 4 is made of elastic emulsion material, and the first liquid changing device piston 4 is connected with the first liquid changing device piston
Bar 5；First liquid changing device 2 has first gear rack gear arrangement 7, and first gear rack gear arrangement 7 includes being fixed on liquid changing device piston rod 5
On First piston bar rack 6 and the first manual gear 8 that is fixed on by circular metal axis 9 on metal axis fixing plate 10.The
The safe volume of maximum of two liquid changing devices 12 is 50mL, has and changes the liquid changing device outlet 11 that liquid pipe 26 is connected with second；Second changes
12 outer surface of liquid device is equipped with liquid changing device scale value 13, is internally provided with the second liquid changing device piston 14, tail portion is fixed equipped with metal shaft
Plate 20；Second liquid changing device piston 14 is made of elastic emulsion material, and the second liquid changing device piston 14 is connected with the second liquid changing device piston rod
15；Second liquid changing device 12 has second gear rack gear arrangement 17, and second gear rack gear arrangement 17 includes being fixed on liquid changing device piston
Second piston bar rack 16 on bar 15 and the second-hand's moving teeth being fixed on by circular metal axis 19 on metal axis fixing plate 20
Wheel 18.Circular metal axis 9 and circular metal axis 19 are prepared by stainless steel material.
Liquid pipe 26 is changed, and the other end of chemical feed pipe 38 is by adding in one end of chemical feed pipe 38 by the connection of quick coupling 30 second
Medicine bottle piston 36 is inserted directly into drug vial 34, there is chemical feed pipe buffering area 37, manually-operated gate 39 and chemical feed pipe 38 on chemical feed pipe 38
Manually-operated gate 39 on have manually-operated gate knob 40；Drug vial 34 has drug vial piston 36 made of elastic emulsion material；Add
One end that there is chemical feed pipe 38 at 36 center of medicine bottle piston is inserted into and is in close contact between drug vial piston 36 and chemical feed pipe 38, drug vial
It is 100mL that, which there is the safe volume of the maximum of drug vial scale value 35 and drug vial scale value 35 in 34 body portions, and 34 bottom of drug vial has ellipse
Justify hanging ring 32 and oval hanging ring 32 is hung on plastics crotch 33, plastics crotch 33 is prepared by polystyrene material and plastics
Crotch 33 is directly sticked on culture roof box 31.
It is as follows using idiographic flow：
During the utility model use, one 45, one first, plated film culture bottle of design changes liquid pipe 24, one second
Change the liquid changing device of bottle cap air filter 25, two of liquid pipe 26, one (the first liquid changing device 2, the second liquid changing device 12), a chemical feed pipe
38th, a drug vial 34 contributes to experimenter to carry out at the uniform velocity change to suspension cell, controllableization changes liquid and ensures that suspension cell changes
The sterilization of liquid process additionally aids experimenter and at the uniform velocity change, controllableization dosing is carried out to suspension cell and is ensured thin to suspending
Drug is sterile filtered added by born of the same parents, is more conducive to routine culture suspension cell and ensures suspension cell always in the culture that suspends
State, reasonable aeration status, final the utility model can realize suspension cell is changed liquid process and dosing process at the uniform velocity change,
Controllableization, sterilization.
(1) conventional liquid feeding is if desired carried out to suspension cell, then needs to install cell culture fluid in charger 12, is twisted
Manually-operated gate knob 40 on tight manually-operated gate 39, tightens the manually-operated gate knob 22 on manually-operated gate 23, unscrews manually-operated gate
Manually-operated gate knob 28 on 29 at this time, it may be necessary to rotate clockwise manual gear 18, drives piston rod rack 16 to move, promotes
Liquid changing device piston rod 15 pushes liquid changing device piston motion, and cell culture fluid is changed by the first liquid changing device 12, second in liquid pipe 26
Change liquid pipe filter 27 into plant cell suspension cultures.
(2) conventional drawing liquid is if desired carried out to suspension cell, then needs to tighten the manually-operated gate knob on manually-operated gate 39
40, the manually-operated gate knob 28 on manually-operated gate 29 is tightened, unscrews the manually-operated gate knob 22 on manually-operated gate 23, needs at this time
Rotary manual gear 8 counterclockwise, drives piston rod rack 6 to move, liquid changing device piston rod 5 is promoted to drive liquid changing device piston motion,
Cell culture fluid is changed into liquid pipe 24 by first, the first liquid changing device 2 is drawn into the first liquid changing device 2.
(3) conventional dosing is if desired carried out to suspension cell, then needs to install drug in drug vial 34, tightens hand-operated valve
Manually-operated gate knob 22 on door 23, tightens the manually-operated gate knob 28 on manually-operated gate 29, unscrews the hand on manually-operated gate 39
Movable valve knob 40, at this time drug enter by drug vial 34, chemical feed pipe 38, the second liquid pipe filter 27 that changes changed in liquid pipe 26
Plant cell suspension cultures.
(4) routine culture if desired is carried out to suspension cell, then needing to tighten has manually-operated gate knob on manually-operated gate 23
22, tightening has manually-operated gate knob 28 on manually-operated gate 29, and tightening has manually-operated gate knob 40 on manually-operated gate 39, opens at the uniform velocity
Tumbler 41 promotes magnetic force at the uniform velocity rotor 42 to keep the speed of 35 turns/min, will keep suspension cell always in suspension
Cultivation conditions.
Final the utility model can change plant cell suspension cultures the at the uniform velocity change, controllably of liquid process and dosing process
Change, sterilization.
Claims (8)
1. a kind of suspension cell combination culture apparatus at the uniform velocity changing liquid and dosing, which is characterized in that including：
Culture bottle, the first liquid changing device, the second liquid changing device and a drug vial；First liquid changing device changes liquid pipe and institute by first
Culture bottle is stated to be connected；Second liquid changing device changes liquid pipe by second and is connected with the culture bottle；Second changes liquid pipe equipped with one
A lateral, the drug vial are connected on the lateral, and then are connected with the culture bottle.
2. the suspension cell combination culture apparatus according to claim 1 at the uniform velocity changing liquid and dosing, it is characterised in that：
Further include a uniform rotation device and an incubator；The culture bottle is installed on the uniform rotation device, training
It supports in bottle and is placed with magnetic force at the uniform velocity rotor；The uniform rotation device is placed on the bottom plate of incubator, and drug vial is suspended on training
On the top plate for supporting case.
3. the suspension cell combination culture apparatus according to claim 1 or 2 at the uniform velocity changing liquid and dosing, it is characterised in that：
The drug vial is connected to by a chemical feed pipe on the lateral；The chemical feed pipe be equipped with dosing buffering area and
Dosing tube valve.
4. the suspension cell combination culture apparatus according to claim 1 at the uniform velocity changing liquid and dosing, it is characterised in that：
Described second, which changes liquid pipe, changes liquid pipe valve equipped with second and changes liquid pipe filter；The lateral is connected to described
Two change liquid pipe valve and change between liquid pipe filter；Described change is equipped with one layer of composite fibre film in liquid pipe filter；Described first
It changes liquid pipe and changes liquid pipe valve equipped with first.
5. the suspension cell combination culture apparatus according to claim 1 at the uniform velocity changing liquid and dosing, it is characterised in that：
The culture bottle is equipped with culture bottle cap；Described first change liquid pipe and second change liquid pipe pass through it is described culture bottle cap with
Culture bottle is connected；Described first changes liquid pipe deeply to culture bottle bottom.
6. the suspension cell combination culture apparatus according to claim 5 at the uniform velocity changing liquid and dosing, it is characterised in that：
The culture bottle has the plated film culture bottle of polymethyl methacrylate film for inner surface, interior to be distributed in equipped with one layer
The silicone hydroxyl film of culture bottle and culture bottle cap inner surface.
7. the suspension cell combination culture apparatus according to claim 5 at the uniform velocity changing liquid and dosing, it is characterised in that：
Bottle cap air filter there are one being set on the culture bottle cap；One layer of 0.45 μm of aperture is equipped in the air filter
Filter membrane and one layer of 0.22 μm of bore filter film.
8. the suspension cell combination culture apparatus according to claim 1 at the uniform velocity changing liquid and dosing, it is characterised in that：
First liquid changing device has the first liquid changing device piston and first manual gear；The first manual gear, which can drive, to be set
In the rack movement on the first liquid changing device piston, liquid purpose is changed so as to reach；Second liquid changing device is lived with the second liquid changing device
Plug and the second manual gear；Second manual gear can drive the rack on the second liquid changing device piston to move, so as to
Reach and change liquid purpose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721691185.0U CN207567247U (en) | 2017-12-07 | 2017-12-07 | At the uniform velocity change liquid and the suspension cell combination culture apparatus of dosing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721691185.0U CN207567247U (en) | 2017-12-07 | 2017-12-07 | At the uniform velocity change liquid and the suspension cell combination culture apparatus of dosing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207567247U true CN207567247U (en) | 2018-07-03 |
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ID=62687277
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721691185.0U Expired - Fee Related CN207567247U (en) | 2017-12-07 | 2017-12-07 | At the uniform velocity change liquid and the suspension cell combination culture apparatus of dosing |
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| CN (1) | CN207567247U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109735453A (en) * | 2019-02-26 | 2019-05-10 | 陕西师范大学 | A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
-
2017
- 2017-12-07 CN CN201721691185.0U patent/CN207567247U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109735453A (en) * | 2019-02-26 | 2019-05-10 | 陕西师范大学 | A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180703 Termination date: 20181207 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |