CN207418761U - Breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus - Google Patents
Breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus Download PDFInfo
- Publication number
- CN207418761U CN207418761U CN201721476379.9U CN201721476379U CN207418761U CN 207418761 U CN207418761 U CN 207418761U CN 201721476379 U CN201721476379 U CN 201721476379U CN 207418761 U CN207418761 U CN 207418761U
- Authority
- CN
- China
- Prior art keywords
- pipette
- pipettor
- small
- breast cancer
- uniform velocity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model discloses a kind of 7 cell lines of breast cancer MCF at the uniform velocity to change liquid culture apparatus；Bottles with flat bases, a liquid storage bottle, the first pipette, the second pipette, liquid relief short tube, the first pipettor and the second pipettor for carrying bottle cap including one；Bottles with flat bases is connected by the first pipette and the second pipette for passing through its bottle cap with the first pipettor and liquid storage device respectively, and liquid storage device is connected by liquid relief short tube with the second pipettor；The front end of first pipette is equipped with the small pin of the first pipette of the deep enough bottles with flat bases of energy, and the front end of second pipette is equipped with the small pin of the second pipette of the deep enough bottles with flat bases of energy.The utility model can be realized at the uniform velocity changes liquid to 7 cell lines of breast cancer MCF, and can ensure to change sterilization, controllableization of liquid process.
Description
Technical field
The utility model is related to breast cancer cell culture technique fields, and in particular to a kind of breast cancer cell line MCF-7 is even
Quick change liquid culture apparatus.
Background technology
One of most common female malignant in breast cancer (breast cancer) whole world accounts for women new cancer sum
25% (epidemiological study [D] the Shandong University of Liu Li beautiful woman Risk Factors for Female Breast Cancer and risk evaluation model,
2015.), therefore, breast cancer prevention and its treatment have important value and meaning.
In general, the basic research for breast cancer is mostly carried out using the method for in vitro culture breast cancer cell MCF-7
(research [D] the Zhengzhou University that Wu Haibo arsenic trioxides act on suspension sacculus culture MCF-7 breast cancer cells, 2013.),
And in vitro culture breast cancer cell MCF-7 needs are often updated the cell culture solution, these, which all refer to have, changes liquid work(
Can, new attached cell blake bottle in vitro culture breast cancer cell MCF-7.
At present, the existing attached cell blake bottle needs in vitro culture breast cancer cell MCF-7 repeatedly unscrew training
Support bottle cap, and need to carry out breast cancer cell MCF-7 multiple culture solution liquid feeding simultaneously, (cell is in adhered state to drawing liquid
Under), this will increase contaminated probability in breast cancer cell MCF-7 incubations, with this be frequently encountered bacterium, virus,
Mycoplasma, Chlamydia, fungi etc. pollution condition, therefore it is necessary to develop it is new, have the function of to change liquid, for external
Cultivate the attached cell blake bottle of breast cancer cell MCF-7.
The content of the invention
For in the prior art the defects of, the purpose of this utility model is to provide a kind of convenient at the uniform velocity changing the breast cancer of liquid
MCF-7 cell lines at the uniform velocity change liquid culture apparatus.
To achieve the above object, the utility model uses following technical scheme：
A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus, which is characterized in that including：
One with the bottles with flat bases of bottle cap, a liquid storage bottle, the first pipette, the second pipette, liquid relief short tube the
One pipettor and the second pipettor；Bottles with flat bases by pass through its bottle cap the first pipette and the second pipette respectively with
One pipettor is connected with liquid storage device, and liquid storage device is connected by liquid relief short tube with the second pipettor；The front end of first pipette
The small pin of the first pipette equipped with the deep enough bottles with flat bases of energy, the front end of second pipette is equipped with can deep enough flat training
Support the small pin of the second pipette of bottle.
Wherein, on the first pipette and pipette filter and pipette filter switch are equipped on the second pipette.
Wherein, two layers of composite fibre film with 0.22 μ m diameter micropore is equipped in the pipette filter switch, it is described
Two layers of composite fibre film between be equipped with 1mm spacing.
Wherein, first pipette include the front and rear small pin filter of the small pin of the first pipette, pipette being sequentially connected,
Pipette switchs and at the uniform velocity pipettor seal nipple；The afterbody of the small pin of first pipette is equipped with the small needle handle of pipette, at the uniform velocity
Pipettor seal nipple is connected with the first pipettor；Second pipette includes the front and rear small pin of the second pipette being sequentially connected, shifting
The small pin filter of liquid pipe and pipette switch；The afterbody of the small pin of pipette is equipped with the small needle handle of pipette.
Wherein, using being threadedly coupled between the bottles with flat bases and its bottle cap, set on the bottle cap there are two latex hole,
It is passed through respectively for the small pin of the first pipette and the small pin of the second pipette.
Wherein, it is not 2cm and 5cm and leading portion and back segment with 140 ° of angles that the small pin of the first pipette is by length
Be formed by connecting, the second liquid relief tubule is respectively 2cm with 5cm by length and the leading portion with 135 ° of angles is connected with back segment and
Into.
Wherein, the bottles with flat bases is adsorbed by an oval silicon rubber suction cup on the side of an incubator；Institute
Liquid storage bottle is stated to be arranged on the bottom surface of the incubator.
Wherein, the head end of the small pin of the first pipette is connected with the first pipette small pinhead, and the first pipette small pinhead is cube
The bodily form, and there are four pipette small pinhead collar extensions for tool.
Wherein, the head end of the small pin of the second pipette is connected with the second pipette small pinhead, and the second pipette small pinhead is cube
The bodily form, and there are six pipette small pinhead collar extensions for tool.
Wherein, the inside of the first pipettor and the second pipettor is equipped with the piston knot that can be driven by manual manipulation gear
Structure and manual manipulation gear.
Compared with prior art, the beneficial benefit of the utility model is：It can aid in even to breast cancer cell line MCF-7
Speed adds in culture solution and is conducive at the uniform velocity extract culture solution to breast cancer cell line MCF-7, contributes to MCF-7 Breast Cancer Cell
Culture solution added by strain is filtered and ensures liquid feeding, controllableization of drawing liquid process, contributes to effectively fixed culture breast cancer MCF-7
The bottles with flat bases of cell line and can use bottles with flat bases mass propgation breast cancer cell line MCF-7, contribute to reduce plus
Liquid, drawing liquid process to the hydraulic shock of breast cancer cell line MCF-7 and promote breast cancer cell line MCF-7 routine adhere-wall culture.
Description of the drawings
Fig. 1 is the structure diagram of the utility model.
Fig. 2 is the front view of the first pipette small pinhead in the present embodiment.
Fig. 3 is the bottom view of the first pipette small pinhead in the present embodiment.
Fig. 4 is the top view of the first pipette small pinhead in the present embodiment.
Fig. 5 is the front view of the second pipette small pinhead in the present embodiment.
Fig. 6 is the bottom view of the second pipette small pinhead in the present embodiment.
Fig. 7 is the top view of the second pipette small pinhead in the present embodiment.
Fig. 8 is oval silicon rubber suction cup and bottles with flat bases combination diagram in the present embodiment.
Fig. 9 is the front view of oval silicon rubber suction cup in the present embodiment.
Figure 10 is bottles with flat bases Bottle cap structure in the present embodiment.
Figure 11 is liquid storage device latex plug structure schematic diagram in the present embodiment.
Reference sign：Incubator side wall 1, oval silicon rubber suction cup 2, bottles with flat bases scale value 3, bottles with flat bases
The small pin 5 of bottleneck thread 4, pipette, the small needle handle 6 of pipette, pipette 7, the small pin filter 8 of pipette, pipette switch 9, shifting
Liquid pipe switch manual button 10, at the uniform velocity pipettor seal nipple 11, pipettor 12, pipettor scale value 13, pipettor piston 14, shifting
Liquid device piston round bar 15, pipettor piston round bar rack 16, pipettor manual gear rack 17, pipettor manual gear 18, shifting
Liquid device gear circular shaft 19, pipettor gear circular shaft stent 20, bottles with flat bases 21, incubator bottom wall 22, the small pin of the first pipette
It is first 23, second pipette small pinhead 24, the small pin 25 of pipette, bottles with flat bases bottleneck 26, bottles with flat bases bottle cap screw thread 27, flat
Bottom culture bottle cap 28, the small needle handle 29 of pipette, pipette 30, the small pin filter 31 of pipette, pipette switch 32, pipette
Switch manual button 33, liquid storage device scale value 34, liquid storage device latex plug 35, incubator bottom wall 36, liquid storage device 37, reservoir outlet are opened
Close 38, reservoir outlet 39, at the uniform velocity liquid relief short tube 40, pipettor seal nipple 41, pipettor 42, pipettor scale value 43, shifting
Liquid device piston 44, pipettor piston round bar 45, pipettor piston round bar rack 46, pipettor manual gear rack 47, pipettor
Manual gear 48, pipettor gear circular shaft 49, pipettor gear circular shaft stent 50, pipette small pinhead internal orifice 51, pipette are small
Syringe needle collar extension 52, pipette small pinhead internal orifice 53, pipette small pinhead collar extension 54, silicon rubber suction cup incubator contact surface 55, silica gel
The flat incubator contact surface 56 of sucker, the small pin latex hole 57 of pipette, the small pin latex hole 58 of pipette, pipette tip breast latex
Hole 59, liquid relief short tube end latex hole 60.
Specific embodiment
As shown in Figure 1, the utility model discloses a kind of breast cancer cell line MCF-7s at the uniform velocity to change liquid culture apparatus, including
Bottles with flat bases 21, pipette 7, pipette 30, liquid relief short tube 40, pipettor 12, pipettor 42, liquid storage device 37 and oval silicon
Glue sucker 2, wherein bottles with flat bases 21 are formed by normal polystyrene material preparation and the floor space of bottles with flat bases 21 is
150cm2, there is bottles with flat bases scale value 3 on 21 surface of bottles with flat bases, and bottles with flat bases scale value 3 shows bottles with flat bases
21 maximum volume is 800mL, and bottles with flat bases 21 is lain against on incubator bottom wall 22 and 21 bottom of bottle of bottles with flat bases has ellipse
Shape silicon rubber suction cup 2.
Oval silicon rubber suction cup 2 has silicon rubber suction cup incubator contact surface 55 and the flat incubator contact surface of silicon rubber suction cup
56, oval silicon rubber suction cup 2 is adsorbed in by silicon rubber suction cup incubator contact surface 55 on incubator side wall 1, and oval silica gel is inhaled
Disk 2 is pasted on 21 bottom of bottles with flat bases by silicon rubber suction cup bottles with flat bases contact surface 56.
Bottles with flat bases bottleneck 26 has bottles with flat bases bottleneck thread 4 and bottles with flat bases bottleneck thread 4 and flat culture
The close occlusive state of screw thread is presented in bottle cap screw thread 27, and bottles with flat bases bottle cap 28 is cylindrical structural and bottles with flat bases bottle cap
28 private sides are stamped bottles with flat bases bottle cap screw thread 27.28 head cover of bottles with flat bases bottle cap has small 57 He of pin latex hole of pipette
The small pin 5 of traveling pipette is distinguished in the small pin latex hole 58 of pipette, the small pin latex hole 57 of pipette and the small pin latex hole 58 of pipette
Pin 25 small with pipette.
Pipette 7 includes the small pin 5 of pipette, the small pin filter 8 of pipette, the pipette switch 9 and at the uniform velocity being sequentially connected
Pipettor seal nipple 11；The afterbody of the small pin 5 of pipette is equipped with the small needle handle 6 of pipette；The preceding segment length of the small pin 5 of pipette is
2cm, the rear segment length of the small pin 5 of pipette are the folder clockwise of 5cm, the leading portion of the small pin 5 of pipette and the back segment of the small pin 5 of pipette
Angle α is 140 °.
Pipette 30 includes the small pin 25 of pipette, the small pin filter 31 of pipette and the pipette switch 32 being sequentially connected；
The afterbody of the small pin 25 of pipette is equipped with the small needle handle 29 of pipette；The preceding segment length of the small pin 25 of pipette be 2cm, the small pin 25 of pipette
Rear segment length for 5cm, the angle β clockwise of the leading portion of the small pin 25 of pipette and the back segment of the small pin 25 of pipette is 135 °.
The head end of the leading portion of the small pin 5 of pipette is connected with the first pipette small pinhead 23, and the first pipette small pinhead 23 is vertical
It is square that cube shape and the first pipette small pinhead 23 the upper surface of, which are seen, to see below the first pipette small pinhead 23 be pros
Shape, it is rectangle that 23 side of the first pipette small pinhead, which is seen, and it is small to be equipped with pipette from the upper surface of the first pipette small pinhead 23
Syringe needle internal orifice 51, pipette small pinhead internal orifice 51 are the liquid inlets being connected with the first pipette small pinhead 23.From the first liquid relief
Pipe small pinhead 23 is observed below, and the first pipette small pinhead 23 has there are four pipette small pinhead collar extension 52, the small pin 5 of pipette
The angle γ clockwise of leading portion and the first pipette small pinhead 23 is 40 °.
The head end of the leading portion of the small pin 25 of pipette is connected with the second pipette small pinhead 24, and the second pipette small pinhead 24 is vertical
It is square that cube shape and the second pipette small pinhead 24 the upper surface of, which are seen, to see below the second pipette small pinhead 24 be pros
Shape, it is rectangle that 24 side of the second pipette small pinhead, which is seen, and it is small to be equipped with pipette from the upper surface of the second pipette small pinhead 24
Syringe needle internal orifice 53, pipette small pinhead internal orifice 53 are the liquid inlets being connected with the second pipette small pinhead 24.From the second liquid relief
Pipe small pinhead 24 is observed below, and there are six pipette small pinhead collar extension 54, the small pins 25 of pipette for the second pipette small pinhead 24 tool
Leading portion and the second pipette small pinhead 24 angle δ clockwise be 45 °.
There is the composite fibre film of 2 layers of 0.22 μm of micro-pore diameter in pipette filter 8, and have between 2 layers of composite fibre film
1mm spacing, pipette, which is switched on 9, pipette switch manual button 10, so that operator controls liquid relief process.
The end of pipette 30 is directly goed deep into liquid storage device 37, and liquid storage device 37 divides for body portion and top；The body of liquid storage device 37
Portion is cylindrical, the top of liquid storage device 37 is cone, and liquid storage device 37 is positioned on incubator bottom wall 36, and 37 capacity of liquid storage device is
150mL and 37 body surface of liquid storage device have liquid storage device scale value 34, and liquid storage device has reservoir outlet 39 and liquid storage below 37 body portion
There is reservoir outlet to switch 38 in device outlet 39,37 top bottleneck of liquid storage device has liquid storage device latex plug 35 and liquid storage device latex plug 35
On have pipette tip latex hole 59 and liquid relief short tube end latex hole 60；Pipette tip latex hole 59, liquid relief short tube end
The end of traveling pipette 30 and the end of liquid relief short tube 40 are distinguished in latex hole 60, and 40 one end of liquid relief short tube is goed deep into liquid storage device 37
And the other end of liquid relief short tube 40 is at the uniform velocity pipettor seal nipple 41.
The at the uniform velocity pipettor seal nipple 11 of pipette 7 (can carry out liquid feeding) is connected with pipettor 12；Liquid relief short tube 40
The at the uniform velocity pipettor seal nipple 41 of (can carry out drawing liquid) is connected with pipettor 42, the capacity of pipettor 8 and pipettor 42
It is 50mL and the surface of pipettor 8 and pipettor 42 is respectively equipped with pipettor scale value 13 and pipettor scale value 43.
Pipettor 8 is internally provided with pipettor piston 14, and pipettor piston round bar 15 is connected with behind pipettor piston 14,
Pipettor piston round bar 15 is equipped with pipettor piston round bar rack 16；And pipettor piston round bar rack 16 is manual with pipettor
Rack-and-pinion 17 is meshed；Pipettor manual gear rack 17 is located on pipettor manual gear 18 and pipettor manual gear 18
Center is pipettor gear circular shaft 19, and pipettor gear circular shaft 19 is located on pipettor gear circular shaft stent 20, pipettor gear
Circular shaft stent 20 is fixed on the tail end of pipettor 8.
Pipettor 42 is internally provided with pipettor piston 44, and pipettor piston round bar is connected with behind pipettor piston 44
45, pipettor piston round bar 45 is equipped with pipettor piston round bar rack 46；And pipettor piston round bar rack 46 and pipettor
Manual gear rack 47 is meshed；Pipettor manual gear rack 47 is located on pipettor manual gear 48 and the manual tooth of pipettor
48 center of wheel is pipettor gear circular shaft 49, and pipettor gear circular shaft 49 is located on pipettor gear circular shaft stent 50, pipettor
Gear circular shaft stent 50 is fixed on the tail end of pipettor 42.
It is as follows using idiographic flow：
During the utility model use, using a bottles with flat bases, two pipettes, a liquid relief short tube and two
A pipettor, primarily at the uniform velocity being changed liquid to breast cancer cell line MCF-7；The effect of oval silicon rubber suction cup 2 is steady
Determine bottles with flat bases, liquid storage device 37 is beneficial to collect breast cancer cell line MCF-7 culture solution at any time.The liquid relief of two pipette front ends
The special construction of pipe small pinhead, the curved design of two pipette syringe needles are beneficial to reduce and change during liquid to breast cancer
The percussion of MCF-7 cell lines.
If not changing liquid to breast cancer cell line MCF-7, need pipette switch manual button 10 and pipette switch hand
Dynamic button 33 is tightened, while unscrews bottles with flat bases bottle cap 28, then can promote breast cancer cell line MCF-7 cellar culture.
If adding in culture solution to breast cancer cell line MCF-7, need to open on pipette switch manual button 10 and pipette
It closes manual button 33 to unscrew, while tightens bottles with flat bases bottle cap 28, breast cancer cell line MCF-7 cultivating system only has the first liquid relief
23 and second pipette small pinhead 24 of pipe small pinhead can add in breast cancer MCF- at this time with being communicated on the outside of bottles with flat bases
7 cell line culture solutions are, it is necessary to equipped with the required culture solution of breast cancer cell line MCF-7 in the pipettor 8, hand counterclockwise at this time
Dynamic pipettor manual gear rack 17, transfers strength by pipettor piston round bar rack 16, pipettor piston 14 is driven to move,
Breast cancer cell line MCF-7 culture solution is along pipettor 12, pipette 7, the small pin 5 of pipette, the first pipette small pinhead at this time
23rd, pipette small pinhead internal orifice 51, pipette small pinhead collar extension 52, breast cancer cell line MCF-7 cultivating system, this process is pair
Breast cancer cell line MCF-7 adds in culture solution.
If extracting culture solution to breast cancer cell line MCF-7, need to unscrew on pipette switch manual button 33, by liquid relief
Pipe switch manual button 10 is tightened while tightens bottles with flat bases bottle cap 28, and breast cancer cell line MCF-7 cultivating system only has second
Pipette small pinhead 24 can be communicated with the outside of bottles with flat bases 21, it is necessary to breast cancer cell line MCF-7 culture solution can be extracted,
Manual liquid transfering device manual gear rack 47 clockwise at this time transfers strength by pipettor piston round bar rack 46, drives liquid relief
Device piston 44 moves, and breast cancer cell line MCF-7 culture solution is along breast cancer cell line MCF-7 cultivating system, pipette at this time
Small pinhead collar extension 54, pipette small pinhead internal orifice 53, the second pipette small pinhead 24, the small pin 25 of pipette, pipette 30, liquid storage
Device 37, this process are to extract culture solution to breast cancer cell line MCF-7.
Final the utility model can add in extracting culture solution breast cancer cell line MCF-7, be realized with this to mammary gland
Cancer MCF-7 cell lines at the uniform velocity change liquid process.
Claims (9)
1. a kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus, which is characterized in that including：
One bottles with flat bases with bottle cap, a liquid storage bottle, the first pipette, the second pipette, liquid relief short tube, first move
Liquid device and the second pipettor；Bottles with flat bases is moved by the first pipette and the second pipette for passing through its bottle cap with first respectively
Liquid device is connected with liquid storage device, and liquid storage device is connected by liquid relief short tube with the second pipettor；The front end of first pipette is equipped with
The small pin of the first pipette of the deep enough bottles with flat bases of energy, the front end of second pipette is equipped with can deep enough bottles with flat bases
The small pin of the second pipette.
2. breast cancer cell line MCF-7 according to claim 1 at the uniform velocity changes liquid culture apparatus, it is characterised in that：
On first pipette and it is equipped with pipette filter and pipette filter switch on the second pipette.
3. breast cancer cell line MCF-7 according to claim 1 or 2 at the uniform velocity changes liquid culture apparatus, it is characterised in that：
Two layers of composite fibre film with 0.22 μ m diameter micropore, described two layers mixing are equipped in the pipette filter switch
The spacing of 1mm is equipped between tunica fibrosa.
4. breast cancer cell line MCF-7 according to claim 1 at the uniform velocity changes liquid culture apparatus, it is characterised in that：
First pipette includes the front and rear small pin filter of the small pin of the first pipette, pipette, the pipette being sequentially connected and opens
It closes and at the uniform velocity pipettor seal nipple；The afterbody of the small pin of first pipette is equipped with the small needle handle of pipette, and at the uniform velocity pipettor is close
Sealing joint is connected with the first pipettor；Second pipette includes the front and rear small pin of the second pipette, the small pin of pipette being sequentially connected
Filter and pipette switch；The afterbody of the small pin of pipette is equipped with the small needle handle of pipette.
5. breast cancer cell line MCF-7 according to claim 1 at the uniform velocity changes liquid culture apparatus, it is characterised in that：
Using being threadedly coupled between the bottles with flat bases and its bottle cap, set on the bottle cap there are two latex hole, respectively for the
The small pin of one pipette and the small pin of the second pipette pass through.
6. breast cancer cell line MCF-7 according to claim 1 at the uniform velocity changes liquid culture apparatus, it is characterised in that：
It is not 2cm and 5cm and leading portion with 140 ° of angles and back segment are formed by connecting that the small pin of first pipette is by length,
The second liquid relief tubule is respectively that 2cm and 5cm and leading portion with 135 ° of angles and back segment are formed by connecting by length.
7. breast cancer cell line MCF-7 according to claim 1 at the uniform velocity changes liquid culture apparatus, it is characterised in that：
The bottles with flat bases is by an oval silicon rubber suction cup absorption on the side of an incubator；The liquid storage bottle is set
It puts on the bottom surface of the incubator.
8. breast cancer cell line MCF-7 according to claim 1 at the uniform velocity changes liquid culture apparatus, it is characterised in that：
The head end of the small pin of first pipette is connected with the first pipette small pinhead, and the first pipette small pinhead is cube shaped, and is had
There are four pipette small pinhead collar extension,
The head end of the small pin of second pipette is connected with the second pipette small pinhead, and the second pipette small pinhead is cube shaped, and is had
There are six pipette small pinhead collar extensions.
9. the breast cancer cell line MCF-7 according to claim 1 or 6 at the uniform velocity changes liquid culture apparatus, it is characterised in that：
The inside of first pipettor and the second pipettor is equipped with the piston structure and hand that can be driven by manual manipulation gear
Dynamic manipulation gear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721476379.9U CN207418761U (en) | 2017-11-08 | 2017-11-08 | Breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721476379.9U CN207418761U (en) | 2017-11-08 | 2017-11-08 | Breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207418761U true CN207418761U (en) | 2018-05-29 |
Family
ID=62307968
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721476379.9U Expired - Fee Related CN207418761U (en) | 2017-11-08 | 2017-11-08 | Breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207418761U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913603A (en) * | 2018-09-26 | 2018-11-30 | 天津市康婷生物工程有限公司 | A kind of recyclable multilayer culture and antipollution attached cell culture apparatus |
| CN109735453A (en) * | 2019-02-26 | 2019-05-10 | 陕西师范大学 | A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
| CN113784618A (en) * | 2019-04-18 | 2021-12-10 | 艾步思国际有限责任公司 | System and process for continuous addition of cryoprotectant |
| CN115926955A (en) * | 2022-12-30 | 2023-04-07 | 浙江安各洛生物技术有限公司 | Auxiliary mixing device for sterilization of multi-component culture medium |
-
2017
- 2017-11-08 CN CN201721476379.9U patent/CN207418761U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913603A (en) * | 2018-09-26 | 2018-11-30 | 天津市康婷生物工程有限公司 | A kind of recyclable multilayer culture and antipollution attached cell culture apparatus |
| CN109735453A (en) * | 2019-02-26 | 2019-05-10 | 陕西师范大学 | A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
| CN113784618A (en) * | 2019-04-18 | 2021-12-10 | 艾步思国际有限责任公司 | System and process for continuous addition of cryoprotectant |
| CN113784618B (en) * | 2019-04-18 | 2023-12-22 | 艾步思国际有限责任公司 | System and process for continuous addition of cryoprotectant |
| US11889830B2 (en) | 2019-04-18 | 2024-02-06 | Abs Global, Inc. | System and process for continuous addition of cryoprotectant |
| CN115926955A (en) * | 2022-12-30 | 2023-04-07 | 浙江安各洛生物技术有限公司 | Auxiliary mixing device for sterilization of multi-component culture medium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207418761U (en) | Breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus | |
| CN206204332U (en) | Cell is centrifuged blake bottle | |
| CN206334690U (en) | Flexible antipollution liquid-transfering gun pipette tips can be turned to | |
| CN203108552U (en) | Multi-purpose chemical reagent bottle | |
| CN207786647U (en) | Liquid-transfering gun pipette tips boxing device | |
| CN204636383U (en) | The quantitative micro blood-taking tube of a kind of single use two-period form | |
| CN214636519U (en) | Adjustable pipettor for pathological analysis | |
| CN205313577U (en) | Aseptic cell culture bottle | |
| CN204638219U (en) | A kind of filtration pipettor | |
| CN209974772U (en) | Suction device for liquid in culture plate | |
| CN203091098U (en) | Medical low-speed centrifuge | |
| CN207567247U (en) | At the uniform velocity change liquid and the suspension cell combination culture apparatus of dosing | |
| CN204474691U (en) | Cell culture bags special filling liquid funnel | |
| CN210410790U (en) | Separable liquid-separating centrifuge tube | |
| CN209810211U (en) | Detachable pipette | |
| CN204050427U (en) | A kind of single use tracheascope liquid accumulating device | |
| CN206986151U (en) | A kind of micro-example transfer tool | |
| CN207012905U (en) | A kind of bottle swingging machine bottle fixing device | |
| CN202437137U (en) | Novel blood collector for blood test | |
| CN207287472U (en) | A kind of continuous liquid-moving machine | |
| CN206239737U (en) | Dispenser | |
| CN203958987U (en) | Detachable portable insect specimen collecting memory device | |
| CN206229385U (en) | New liquid-transfering gun pipette | |
| CN205060345U (en) | Bottle plug convenient to liquid medicine extraction | |
| CN203101123U (en) | Novel urine collector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180529 Termination date: 20191108 |