CN207552323U - A kind of solid state fermentation culture bottle - Google Patents
A kind of solid state fermentation culture bottle Download PDFInfo
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- CN207552323U CN207552323U CN201721613512.0U CN201721613512U CN207552323U CN 207552323 U CN207552323 U CN 207552323U CN 201721613512 U CN201721613512 U CN 201721613512U CN 207552323 U CN207552323 U CN 207552323U
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- bottle
- bottle body
- solid state
- diameter
- state fermentation
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Abstract
The utility model is related to a kind of solid state fermentation culture bottle, including bottle body, bottleneck is located at the top of bottle body, and bottom of bottle is located at the bottom of bottle body, and the diameter of bottleneck is less than the diameter of bottom of bottle, and the ratio of the diameter of bottleneck and the height of bottle body is (4 6)：The ratio of (10 15), the diameter of bottom of bottle and the height of bottle body is 12：(10‑15).The utility model have many advantages, such as it is easy to operate, be not easy to contaminate miscellaneous bacteria and save the operating time.
Description
Technical field
The utility model belongs to bioengineering field more particularly to a kind of solid state fermentation culture bottle.
Background technology
Solid state fermentation refer to microorganism without or the basic solid state substrate without free water on fermentation method, solid-state
It gas, liquid, solid three-phase and is deposited in matrix, i.e., contains water and water-insoluble materials in porous solid state substrate.It is more during solid state fermentation
Using culture plate or solid state fermentation device.Culture plate is mostly in oblate cylindricality, on the one hand since its capacity is limited, so general use
In the culture of seed, on the other hand, opening is larger, in operation, culture medium in culture plate and extraneous contact surface compared with
Greatly, so the probability of dye miscellaneous bacteria is larger.Solid state fermentation device equipment is complicated and expensive, is generally used for training of fermenting on a large scale
It supports.For existing triangular flask for Liquid Culture fermentation process designs, opening is smaller and in thin-high shape, is inoculated in solid culture
When it is inconvenient, be not easy seed, and the side wall of oese is easy to encounter the inner wall of triangular flask, if wanting to avoid dye miscellaneous bacteria,
The calcination sterilizing on alcolhol burner by oese whole (bar portion including oese) is needed during operation, extends the operating time.
Utility model content
In view of the problems of prior art, the utility model provides a kind of solid state fermentation culture bottle, has operation
Conveniently, the advantages that being not easy to contaminate miscellaneous bacteria and save the operating time.
The technical solution that the utility model solves above-mentioned technical problem is as follows：
A kind of solid state fermentation culture bottle, including bottle body, bottleneck is located at the top of bottle body, and bottom of bottle is located at bottle body
Bottom, the diameter of bottleneck are less than the diameter of bottom of bottle, and the ratio of the diameter of bottleneck and the height of bottle body is (4-6)：(10-15)；
The ratio of the diameter of bottom of bottle and the height of bottle body is 12：(10-15).
The beneficial effects of the utility model are：Solid state fermentation culture bottle provided by the utility model, have it is easy to operate,
The advantages that being not easy to contaminate miscellaneous bacteria and save the operating time.
Ratio between the height three of the diameter of bottleneck, the diameter of bottom of bottle and bottle body is suitable, is conducive to facilitate experiment
It operates and provides suitable culture environment and growing space for microorganism.If the height of bottle body is too high, oese is deeply trained
The part or more in bottle is supported, easily entrain miscellaneous bacteria or needs to extend the sterilization time of oese, conventional efficient reduces.Bottleneck
Diameter and bottle body height ratio it is suitable, stretched in culture bottle convenient for experimental apparatus such as oeses, it is easy to operation.Such as
The diameter of fruit bottleneck is excessive, and culture medium moisture loss is larger during culture is unfavorable for cultivating, and contaminating the probability of miscellaneous bacteria also increases.
Based on the above technical solution, the utility model can also do following improvement.
Further, handle is further included, is additionally provided on bottle body and the matching used cyclic structure of handle, the number of cyclic structure
It is two to measure, and two cyclic structures are symmetricly set on the lateral wall of bottle body, and the bottom of handle is in the hook solid being bent upwards,
The bottom of handle can be penetrated to being used in cyclic structure that culture bottle to be lifted or be put down.
Advantageous effect using above-mentioned further scheme is：Culture bottle easy to remove.In particular, after sterilizing, culture
The temperature of bottle is higher, if culture bottle of directly being taken with hand can relatively scald one's hand.It, can be by the bottom of handle after handle
It penetrates to cyclic structure, culture bottle can be moved directly up by lifting, easy to operate and avoid scalding one's hand.
Further, the material of culture bottle is glass or high-temperature resistance plastice.
Advantageous effect using above-mentioned further scheme is：Convenient for sterilizing etc. operations, using finish cleaning after can repeat
It uses, saves experimental cost.
Further, bottle stopper is further included, bottle stopper is removably mounted on bottle mouth position.
Advantageous effect using above-mentioned further scheme is：Dye miscellaneous bacteria is on the one hand prevented using bottle stopper, it is on the other hand removable
The setting unloaded is conveniently operated.
Further, the bottle stopper includes plug ontology, breathable envelope bottle film and outer cover, and the inside for filling in ontology has through-hole,
Through-hole seals bottle film and is arranged on the upper surface of plug ontology and covers through-hole through the upper and lower surface of plug ontology；Outer cover
Be sleeved on plug outer body, the inner wall of outer cover and the outer wall of plug ontology formed for accommodate bottle body bottle wall space；Solid is sent out
During ferment culture, plug ontology is located in bottle body, and outer cover is located at outside bottle body.
Advantageous effect using above-mentioned further scheme is：In use, plug ontology is filled in bottle body, bottle body is located at
In the space that the outer wall of the inner wall of outer cover and plug ontology is defined as, outer cover is located at outside bottle body, and plug ontology, which plays, blocks bottleneck
Purpose, plug ontology is equipped with through-hole, while while dye miscellaneous bacteria is effectively avoided, avoids going out covered with envelope bottle film on upper surface
The phenomenon that being come off during bacterium due to bottle stopper caused by pressure rise in bottle body.Outer cover forms protection to plug ontology, when
Since when experiment, which needs to take out from bottleneck, fills in ontology, the bacterium of external environment is filled in since the effect of blocking of outer cover will not be fallen
In ontology.
Further, it is silica gel to fill in ontology and the material of outer cover.
Advantageous effect using above-mentioned further scheme is：It can be adapted for the operations such as sterilizing, meanwhile, silica gel has certain bullet
Property, ensure that bottle stopper can be firmly covered in bottle mouth position.
Description of the drawings
Fig. 1 is the overall structure diagram of solid state fermentation culture bottle described in the utility model；
Fig. 2 is the structure diagram of bottle body described in the utility model；
Fig. 3 is the front view of handle described in the utility model；
Fig. 4 is the side view of handle described in the utility model；
Fig. 5 is the vertical view of bottle stopper described in the utility model；
Fig. 6 is the sectional view at A-A of handle described in the utility model.
In attached drawing, parts list represented by the reference numerals are as follows：
1st, bottle body, 11, bottleneck, 12, bottom of bottle, 13, cyclic structure, 2, handle, 3, bottle stopper, 31, plug ontology, 311, logical
Hole, 32, envelope bottle film, 33, outer cover.
Specific embodiment
The principle and feature of the utility model are described below in conjunction with attached drawing, example is served only for explaining this practicality
It is novel, it is not intended to limit the scope of the utility model.
As depicted in figs. 1 and 2, a kind of solid state fermentation culture bottle, including bottle body 1, bottleneck 11 is located at the top of bottle body 1
Portion, bottom of bottle 12 are located at the bottom of bottle body 1, and the diameter (D1) of bottleneck 11 is less than the diameter (D2) of bottom of bottle 12, the diameter of bottleneck 11
(D1) and the ratio of the height of bottle body 1 (H) is (4-6)：(10-15).The diameter (D2) of bottom of bottle 12 and the height of bottle body 1
(H) ratio is 12：(10-15).The material of culture bottle is glass or high-temperature resistance plastice (at least resistance to 130 DEG C of high-temperature resistance plastice
High temperature can meet the temperature requirement of sterilizing).
In the utility model, the diameter (D1) of bottleneck 11 refers to 1 open-topped diameter of bottle body.The diameter of bottom of bottle 12
(D2) diameter that bottom of bottle 12 is projected to horizontal plane under normal operating condition is referred to.The height (H) of bottle body 1 refers to bottle sheet
Vertical range between the top and lowermost end of body 1.
Shown as shown in Figure 1, Figure 3 and Figure 4, solid state fermentation further includes handle 2 with culture bottle, also has on bottle body 1 and matches with handle 2
The cyclic structure 13 used is covered, the quantity of cyclic structure 13 is two, and two cyclic structures 13 are symmetricly set on the outer of bottle body 1
On side wall, the bottom of handle 2 is in the hook solid being bent upwards, and the bottom of handle 2, which can be penetrated to cyclic structure 13 is interior, to be used to train
Foster bottle lifts or puts down.
As shown in Figure 5 and Figure 6, solid state fermentation further includes bottle stopper 3 with culture bottle, and bottle stopper 3 is removably mounted on bottleneck 11
Place.The bottle stopper 3, which includes plug ontology 31, breathable envelope bottle film 32 and outer cover 33, the inside of plug ontology 31, has through-hole 311,
Through-hole 311 seals bottle film 32 and is arranged on the upper surface of plug ontology 31 and covers through the upper and lower surface of plug ontology 31
Through-hole 311；Outer cover 33 is sleeved on 31 outside of plug ontology, and the inner wall of outer cover 33 and the outer wall of plug ontology 31 form to accommodate bottle body
The space of 1 bottle wall；During solid fermentation culture, outer cover 33 is located at the top of cyclic structure 13, and plug ontology 31 is located at bottle body 1
Interior, outer cover 33 is located at outside bottle body 1.The material for filling in ontology 31 and outer cover 33 is silica gel.
The advantages of for the technical program is explained further, is introduced below by some specific embodiments and comparative example.
Embodiment 1
As shown in Figures 1 to 6, solid state fermentation includes bottle body 1, handle 2 and bottle stopper 3 with culture bottle.
As depicted in figs. 1 and 2, from the side, bottle body 1 is in triangular shape.The lower part of bottle body 1 is round platform, bottle body 1
Top is cylinder, and bottleneck 11 is located at the top (i.e. the top of cylinder) on the top of bottle body 1, and bottom of bottle 12 is located under bottle body 1
The bottom in portion, the diameter (D1) of bottleneck 11 is less than the diameter (D2) of bottom of bottle 12, the diameter (D1) of bottleneck 11 and the height of bottle body 1
(H) ratio is 4：15.The ratio of the diameter (D2) of bottom of bottle 12 and the height (H) of bottle body 1 is 12：15.The material of culture bottle
For glass or high-temperature resistance plastice (high temperature that at least resistance to 130 DEG C of high-temperature resistance plastice can meet the temperature requirement of sterilizing).
It is shown as shown in Figure 1, Figure 3 and Figure 4, it is additionally provided on bottle body 1 and 2 matching used cyclic structure 13 of handle, ring-type knot
The quantity of structure 13 is two, and two cyclic structures 13 are symmetricly set on the lateral wall of bottle body 1, and the bottom of handle 2 is in upward
The hook solid of bending, the bottom of handle 2, which can be penetrated to cyclic structure 13 is interior, is used to that culture bottle to be lifted or be put down.
As shown in Figure 5 and Figure 6, bottle stopper 3 is removably mounted at bottleneck 11.The bottle stopper 3 include plug ontology 31, can be saturating
The envelope bottle film 32 and outer cover 33 of gas, plug ontology 31 inside have through-hole 311, through-hole 311 through plug ontology 31 upper surface and
Lower surface, envelope bottle film 32 are arranged on the upper surface of plug ontology 31 and cover through-hole 311；Outer cover 33 is sleeved on outside plug ontology 31
The outer wall of side, the inner wall of outer cover 33 and plug ontology 31 formed for accommodate bottle body 1 bottle wall space；Solid fermentation culture
When, outer cover 33 is located at the top of cyclic structure 13, and plug ontology 31 is located in bottle body 1, fill in outer wall at least part of ontology 31 with
The inner wall contact of bottle body 1, envelope bottle film 32 just get lodged in the bottle mouth position of bottle body 1, and outer cover 33 is located at outside bottle body 1, outer cover 33
Inner wall at least partly contacted with the outer wall of bottle body 1.The material for filling in ontology 31 and outer cover 33 is silica gel.
Embodiment 2
The ratio of the diameter (D1) of bottleneck 11 and the height (H) of bottle body 1 is 5：12.The diameter (D2) and bottle sheet of bottom of bottle 12
The ratio of the height (H) of body 1 is 12：12.Remaining is same as Example 1.
Embodiment 3
The ratio of the diameter (D1) of bottleneck 11 and the height (H) of bottle body 1 is 6：10.The diameter (D2) and bottle sheet of bottom of bottle 12
The ratio of the height (H) of body 1 is 12：10.Remaining is same as Example 1.
In comparative example 1, the ratio of the diameter (D1) of bottleneck 11 and the height (H) of bottle body 1 is 2：15.Remaining with implementation
Example 1 is identical.In comparative example 2, the ratio of the diameter (D1) of bottleneck 11 and the height (H) of bottle body 1 is 3：15.Remaining with implementation
Example 1 is identical.In comparative example 3, the ratio of the diameter (D1) of bottleneck 11 and the height (H) of bottle body 1 is 7：15.Remaining with implementation
Example 1 is identical.In comparative example 4, the ratio of the diameter (D1) of bottleneck 11 and the height (H) of bottle body 1 is 8：15.Remaining with implementation
Example 1 is identical.
The culture that the culture bottle of the various embodiments described above and each comparative example is used to produce to the microorganism of antibiotic respectively is real
It tests.The culture medium of equal thickness is added in into each culture bottle respectively.The culture bottle solid state fermentation of Example 1 100 times, Example
2 culture bottle solid state fermentation 100 times, the culture bottle solid state fermentation of Example 3 100 times take the culture bottle solid-state of comparative example 1 to send out
Ferment 100 times takes the culture bottle solid state fermentation 100 times of comparative example 2, takes the culture bottle solid state fermentation 100 times of comparative example 3, takes comparison
The culture bottle solid state fermentation of example 4 100 times.And count the dye miscellaneous bacteria rate of each group embodiment and comparative example and the bar portion touching of oese
The number of culture bottle inner wall.
Experimental result is as shown in table 1.
Table 1
| Contaminate miscellaneous bacteria rate (%) | The number of the bar portion touching culture bottle inner wall of oese | |
| Embodiment 1 | 0 | 1 |
| Embodiment 2 | 0 | 0 |
| Embodiment 3 | 0 | 2 |
| Comparative example 1 | 3 | 11 |
| Comparative example 2 | 2 | 10 |
| Comparative example 3 | 1 | 0 |
| Comparative example 4 | 5 | 0 |
Can be seen that comparative example 1 to comparative example 4 from the data of table 1 has different degrees of dye miscellaneous bacteria rate, is above implementing
Example 1 is to embodiment 3.It has surprisingly been found that, even if connecing identical microorganism, the bacterium colony of embodiment 1 to embodiment 3 is grown under study for action
Situation will be significantly better than comparative example 1 to the growing state of comparative example 4.In conclusion the utility model embodiment 1 is to embodiment 3
Have many advantages, such as that setting is reasonable, easy to operate, be not easy to contaminate miscellaneous bacteria and save the operating time.
The above is only the preferred embodiment of the present invention, is not intended to limit the utility model, all in this practicality
Within novel spirit and principle, any modification, equivalent replacement, improvement and so on should be included in the guarantor of the utility model
Within the scope of shield.
Claims (6)
1. a kind of solid state fermentation culture bottle, which is characterized in that including bottle body (1), bottleneck (11) is positioned at the top of bottle body (1)
Portion, for bottom of bottle (12) positioned at the bottom of bottle body (1), the diameter of bottleneck (11) is less than the diameter of bottom of bottle (12), bottleneck (11) it is straight
The ratio of the height of diameter and bottle body (1) is (4-6)：(10-15)；The ratio of the diameter of bottom of bottle (12) and the height of bottle body (1)
Example is 12：(10-15).
2. a kind of solid state fermentation culture bottle according to claim 1, which is characterized in that further include handle (2), bottle body
(1) be additionally provided on handle (2) matching used cyclic structure (13), the quantity of cyclic structure (13) is two, two ring-types
Structure (13) is symmetricly set on the lateral wall of bottle body (1), and the bottom of handle (2) is in the hook solid being bent upwards, handle (2)
Bottom can penetrate to being used in cyclic structure (13) that culture bottle to be lifted or be put down.
3. a kind of solid state fermentation culture bottle according to claim 1, which is characterized in that the material of bottle body (1) is glass
Or high-temperature resistance plastice.
4. according to a kind of any one of claim 1-3 solid state fermentation culture bottles, which is characterized in that bottle stopper (3) is further included,
Bottle stopper (3) is removably mounted at bottleneck (11).
5. a kind of solid state fermentation culture bottle according to claim 4, which is characterized in that the bottle stopper (3) is including filling in ontology
(31), breathable envelope bottle film (32) and outer cover (33), the inside of plug ontology (31) have through-hole (311), and through-hole (311) runs through
The upper and lower surface of ontology (31) is filled in, envelope bottle film (32) is arranged on the upper surface of plug ontology (31) and covers through-hole
(311)；Outer cover (33) is sleeved on the outside of plug ontology (31), and the inner wall of outer cover (33) forms to accommodate with the outer wall of plug ontology (31)
The space of the bottle wall of bottle body (1)；During solid fermentation culture, plug ontology (31) is in bottle body (1), and outer cover (33) is positioned at bottle
Ontology (1) is outside.
6. a kind of solid state fermentation culture bottle according to claim 5, which is characterized in that plug ontology (31) and outer cover (33)
Material is silica gel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721613512.0U CN207552323U (en) | 2017-11-28 | 2017-11-28 | A kind of solid state fermentation culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721613512.0U CN207552323U (en) | 2017-11-28 | 2017-11-28 | A kind of solid state fermentation culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207552323U true CN207552323U (en) | 2018-06-29 |
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ID=62677525
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721613512.0U Expired - Fee Related CN207552323U (en) | 2017-11-28 | 2017-11-28 | A kind of solid state fermentation culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207552323U (en) |
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2017
- 2017-11-28 CN CN201721613512.0U patent/CN207552323U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180629 Termination date: 20181128 |
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| CF01 | Termination of patent right due to non-payment of annual fee |