CN207537460U - A kind of experiment nucleic acid extracting instrument - Google Patents

Abstract

The utility model is related to molecular biosciences technical fields, and disclose a kind of experiment nucleic acid extracting instrument, including pedestal, the surface of pedestal is installed with support plate, the right side of support plate is equipped with purification process platform and cracking operation platform, and cracking operation platform is located at the surface of purification process platform, and purification process platform and cracking operation platform offer through-hole, the upper end of the through-hole of purification process platform is equipped with purification devices, and cracker is equipped with above the through-hole of cracking operation platform.The experiment nucleic acid extracting instrument, by the way that cracking operation platform and purification process platform are placed up and down, liquid transition is realized using gravity, it eliminates and frequently uses pipettor and replace the cumbersome of test tube, by setting purification devices, by energization solenoid generate magnetic field magnetic bead is attached on adsorption plate, the liquid so as to be convenient for changing in long-neck reaction bulb and will not magnetic bead loss.

Description

A kind of experiment nucleic acid extracting instrument

Technical field

The utility model is related to molecular biosciences technical field, specially a kind of experiment nucleic acid extracting instrument.

Background technology

The common method of nucleic acid extraction has paramagnetic particle method and strong salty method, and wherein paramagnetic particle method receives superparamagnetism with nanometer technology After the surface of rice grain carries out improvement and surface modification, superparamagnetism silica nanometer magnetic bead is prepared into, which can be microcosmic It specifically identifies with nucleic acid molecules and is efficiently combined on interface, using the superparamagnetism of silica nanoparticle, Chaotropic salt（Guanidine hydrochloride, guanidinium isothiocyanate etc.）It, can be from blood, animal tissue, food, disease under the action of externally-applied magnetic field DNA and RNA in pathogenic microorganism equal samples are separated, can be applicable to clinical disease diagnosis, transfusion safety, Forensic Identification, The multiple fields such as environmental microorganism detection, food safety detection, molecular biology research.In the prior art, method for extracting nucleic acid Mostly using instrument for extracting nucleic acid, for experimental study, cost is too big, and uses artificial extraction, because reciprocal transfer magnetic bead is easy The loss of magnetic bead is caused, operating personnel, which frequently replace test tube and liquid drawing device, can also reduce its extraction efficiency, and store test bottle Very big space is also required to test tube.

Utility model content

（One）The technical issues of solution

In view of the deficiencies of the prior art, the utility model provides a kind of experiment nucleic acid extracting instrument, have instrument into Originally largely loss that is relatively low, preventing magnetic bead simplifies the advantages that operation, saving laboratory table space, solves experimental cost height, magnetic bead It easily loses, operating efficiency is low, occupies the problems such as laboratory table space.

（Two）Technical solution

To realize above-mentioned reduction experimental cost, a large amount of losses for preventing magnetic bead, the mesh for simplifying operation, saving laboratory table space , the utility model provides following technical solution：A kind of experiment nucleic acid extracting instrument, including pedestal, the surface of the pedestal Support plate is installed with, the right side of the support plate is equipped with purification process platform and cracking operation platform, and cracking operation platform position In the surface of purification process platform, the purification process platform and cracking operation platform offer through-hole, the cracking operation platform Side is hinged at by hinge and support plate, and the upper end of the through-hole of the purification process platform is equipped with purification devices, described to split It solves and cracker is equipped with above the through-hole of operation console, the through-hole of the purification process platform is arranged right below waste liquid bottle, and waste liquid Bottle is positioned over the upper surface of pedestal, and the upper surface of the pedestal is placed with nucleic acid solution bottle on the right side of waste liquid bottle.

Preferably, the purification devices include purifying bearing, and purify bearing and be located at end edge on the through-hole of purification process platform At edge, the upper end of the purifying bearing is equipped with long-neck reaction bulb, and the long-neck reaction bulb is equipped with liquid valve, institute close to neck mouth position The medial surface for stating long-neck reaction bulb is equipped with adsorption plate, and the long-neck reaction bulb lateral surface is equipped with energization solenoid.

Preferably, the energization solenoid lateral surface is enclosed with transparent shield.

Preferably, the cracker includes cracking bearing, and cracks the upper end that bearing is located at the through-hole of cracking operation platform Edge, the upper end of the cracking bearing are equipped with long neck funnel, leakage valve are equipped at the neck mouth of the long neck funnel.

Preferably, the outer surface of the long neck funnel is equipped with circular heating tube.

Preferably, the through-hole on the purification process platform is arranged with the through-hole dislocation on cracking operation platform.

（Three）Advantageous effect

Compared with prior art, the utility model provides a kind of experiment nucleic acid extracting instrument, has following beneficial to effect Fruit：

1st, the experiment nucleic acid extracting instrument by the way that cracking operation platform and purification process platform are placed up and down, utilizes gravity It realizes liquid transition, eliminates and frequently use pipettor and replace the cumbersome of test tube.

2nd, the experiment nucleic acid extracting instrument, by setting purification devices, the magnetic field generated by energization solenoid is by magnetic Pearl is attached on adsorption plate, the liquid so as to be convenient for changing in long-neck reaction bulb and will not magnetic bead loss, it is transparent by setting Protective cover can both protect energization solenoid, and the personnel that can also be conveniently operated understand the situation in long-neck reaction bulb.

3rd, the experiment nucleic acid extracting instrument by setting circular heating tube, can accelerate the cracking of sample cell, improve effect Rate, the dislocation arrangement of upper lower through-hole, the liquid be conducive in long neck funnel slowly flow into length along the inner wall of long-neck reaction bulb In neck reaction bulb, liquid splash is not generated.

4th, experiment nucleic acid extracting instrument, by the way that cracking operation platform is hinged with support plate, so as to conveniently move out of purifying The space of operation console is conveniently operated personnel to liquid agitation mixing in long-neck reaction bulb.

Description of the drawings

FIG. 1 is a schematic structural view of the utility model；

Fig. 2 is the utility model section schematic diagram；

Fig. 3 is enlarged drawing at A in the utility model Fig. 2；

Fig. 4 is enlarged drawing at B in the utility model Fig. 2.

In figure：1 pedestal, 2 support plates, 3 purification process platforms, 4 cracking operation platforms, 5 through-holes, 6 purification devices, 61 purifying branch Seat, 62 long-neck reaction bulbs, 63 liquid valves, 64 adsorption plates, 65 energization solenoids, 7 crackers, 71 cracking bearings, the leakage of 72 long-necks Bucket, 73 leakage valves, 74 circular heating tubes, 8 waste liquid bottles, 9 nucleic acid solution bottles.

Specific embodiment

The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out It clearly and completely describes, it is clear that the described embodiments are only a part of the embodiments of the utility model rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are without making creative work All other embodiments obtained shall fall within the protection scope of the present invention.

- 4 are please referred to Fig.1, a kind of experiment nucleic acid extracting instrument, including pedestal 1, the surface of pedestal 1 is installed with branch Fagging 2, the right side of support plate 2 are equipped with purification process platform 3 and cracking operation platform 4, and cracking operation platform 4 is located at purification process platform 3 surface by the way that cracking operation platform 4 and purification process platform are placed about 3, is realized liquid transition using gravity, is eliminated It frequently uses pipettor and replaces the cumbersome of test tube, purification process platform 3 and cracking operation platform 4 offer through-hole 5, purification process Through-hole 5 on platform 3 arranges that the dislocation arrangement of upper lower through-hole 5 is conducive to long neck funnel with the dislocation of through-hole 5 on cracking operation platform 4 Liquid in 72 is slowly flowed into along the inner wall of long-neck reaction bulb 62 in long-neck reaction bulb 62, does not generate liquid splash, cracking The side of operation console 4 is hinged at by hinge and support plate 2, and the opposite side of cracking operation platform 4 is equipped with tight with support plate 2 Buckle part by the way that cracking operation platform 4 and support plate 2 are hinged, so as to conveniently move out of the space of purification process platform 3, is conveniently operated Personnel are equipped with purification devices 6, purifying dress to liquid agitation mixing in long-neck reaction bulb 62, the upper end of the through-hole 5 of purification process platform 3 It puts 6 and includes purifying bearing 61, and purify bearing 61 and be located at 5 upper end-face edge of through-hole of purification process platform 3, purify the upper of bearing 61 End is equipped with long-neck reaction bulb 62, and long-neck reaction bulb 62 is equipped with liquid valve 63, the medial surface of long-neck reaction bulb 62 close to neck mouth position Equipped with adsorption plate 64,62 lateral surface of long-neck reaction bulb is equipped with energization solenoid 65, and 65 lateral surface of energization solenoid is enclosed with transparent Magnetic bead by setting purification devices 6, is attached on adsorption plate 64 by protective cover by the magnetic field that energization solenoid 65 generates, when When connecting energization solenoid 65, a magnetic field from bottom to top can be generated inside solenoid, magnetic bead is driven to move to absorption On plate 64, liquid so as to be convenient for changing in long-neck reaction bulb 62 and will not magnetic bead loss, by setting transparent shield, both Energization solenoid 65 can be protected, the personnel that can also be conveniently operated understand the situation in long-neck reaction bulb 62, cracking operation platform 4 5 top of through-hole is equipped with cracker 7, and cracker 7 includes cracking bearing 71, and cracks bearing 71 and be located at cracking operation platform 4 At the upper end-face edge of through-hole 5, the upper end of cracking bearing 71 is equipped with long neck funnel 72, and leakage valve is equipped at the neck mouth of long neck funnel 72 73, the outer surface of long neck funnel 72 is equipped with circular heating tube 74, by setting circular heating tube 74, can accelerate splitting for sample cell Solution, improves efficiency, and the through-hole 5 of purification process platform 3 is arranged right below waste liquid bottle 8, and waste liquid bottle 8 is positioned over the upper table of pedestal 1 Face, the upper surface of pedestal 1 are placed with nucleic acid solution bottle 9 close to the right side of waste liquid bottle 8.

During work, liquid valve 63 and leakage valve 73 are closed, nucleic acid samples liquid is placed in long neck funnel 72, adds in cracking Liquid, energization circular heating tube 74 stand heating 5-10 minutes, slowly open leakage valve 73, treat that lysate sample liquid fully enters length In neck reaction bulb 62, circular heating tube 74 is closed, rotation cracking operation platform 4 adds in magnetic bead combination liquid into long-neck reaction bulb 62, It stirs evenly, stands 20 minutes, after magnetic bead is fully combined with nucleic acid, connect the power supply of energization solenoid 65, stand 5-10 points Clock opens liquid valve 63, and waste liquid is flowed into waste liquid bottle 8, closes liquid valve 63, cleaning solution is added in into long-neck reaction bulb 62, The power supply of energization solenoid 65 is disconnected, is stirred and evenly mixed, stands 5-10 minutes, connects the power supply of energization solenoid 65, stands 5-10 After minute, liquid valve 63 is opened, waste liquid is flowed into waste liquid bottle 8, closes liquid valve 63, elution is added in into long-neck reaction bulb 62 Liquid disconnects the power supply of energization solenoid 65, stirs evenly, and stands 5-10 minutes, connects the power supply of energization solenoid 65, stands 5- It 10 minutes, changes waste liquid bottle 8 into nucleic acid solution bottle 9, opens liquid valve 63, the nucleic acid solution after purification of extraction flows into nucleic acid solution In bottle 9, liquid valve 63 is closed, magnetic bead collection liquid is added in into long-neck reaction bulb 62, disconnects the power supply of energization solenoid 65, is stood 5 minutes, liquid valve 63 is opened, collects magnetic bead solution.

The electric elements occurred in this article are electrically connected with extraneous main controller and 220V alternating currents, and main controller can be meter Calculation machine etc. plays the conventionally known equipment of control.

In conclusion the experiment nucleic acid extracting instrument, by the way that cracking operation platform 4 and purification process platform are placed about 3, Liquid transition is realized using gravity, is eliminated and is frequently used pipettor and replace the cumbersome of test tube；By setting purification devices 6, Magnetic bead is attached on adsorption plate 64 by the magnetic field that energization solenoid 65 generates, so as to be convenient for changing in long-neck reaction bulb 62 Liquid and will not magnetic bead loss, by setting transparent shield, can both protect energization solenoid 65, can also be conveniently operated Personnel understand the situation in long-neck reaction bulb 62；By setting circular heating tube 74, the cracking of sample cell can be accelerated, improve effect Rate, the dislocation arrangement of upper lower through-hole 5, the liquid be conducive in long neck funnel 72 are slowly flowed along the inner wall of long-neck reaction bulb 62 Enter in long-neck reaction bulb 62, do not generate liquid splash；It is pure so as to conveniently move out by the way that cracking operation platform 4 and support plate 2 are hinged Change the space of operation console 3, be conveniently operated personnel to liquid agitation mixing in long-neck reaction bulb 62.

It should be noted that herein, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any this practical relationship or sequence.Moreover, term " comprising ", "comprising" or its any other variant are intended to Non-exclusive inclusion, so that process, method, article or equipment including a series of elements not only will including those Element, but also including other elements that are not explicitly listed or further include as this process, method, article or equipment Intrinsic element.

While there has been shown and described that the embodiment of the utility model, for the ordinary skill in the art, It is appreciated that can these embodiments be carried out with a variety of variations in the case of the principle and spirit for not departing from the utility model, repaiied Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.

Claims (6)

1. a kind of experiment nucleic acid extracting instrument, including pedestal（1）, it is characterised in that：The pedestal（1）Surface be fixedly mounted There is support plate（2）, the support plate（2）Right side be equipped with purification process platform（3）With cracking operation platform（4）, and cracking operation Platform（4）Positioned at purification process platform（3）Surface, the purification process platform（3）With cracking operation platform（4）Offer through-hole （5）, the cracking operation platform（4）Side pass through hinge and support plate（2）Be hinged at, the purification process platform（3）'s Through-hole（5）Upper end be equipped with purification devices（6）, the cracking operation platform（4）Through-hole（5）Top is equipped with cracker（7）, institute State purification process platform（3）Through-hole（5）Be arranged right below waste liquid bottle（8）, and waste liquid bottle（8）It is positioned over pedestal（1）Upper table Face, the pedestal（1）Upper surface close to waste liquid bottle（8）Right side be placed with nucleic acid solution bottle（9）.

2. a kind of experiment nucleic acid extracting instrument according to claim 1, it is characterised in that：The purification devices（6）Packet Include purifying bearing（61）, and purify bearing（61）Positioned at purification process platform（3）Through-hole（5）At upper end-face edge, the purifying branch Seat（61）Upper end be equipped with long-neck reaction bulb（62）, the long-neck reaction bulb（62）Liquid valve is equipped with close to neck mouth position（63）, The long-neck reaction bulb（62）Medial surface be equipped with adsorption plate（64）, the long-neck reaction bulb（62）Lateral surface is equipped with energization helical Pipe（65）.

3. a kind of experiment nucleic acid extracting instrument according to claim 2, it is characterised in that：The energization solenoid（65） Lateral surface is enclosed with transparent shield.

4. a kind of experiment nucleic acid extracting instrument according to claim 1, it is characterised in that：The cracker（7）Packet Include cracking bearing（71）, and crack bearing（71）Positioned at cracking operation platform（4）Through-hole（5）Upper end-face edge at, the cracking Bearing（71）Upper end be equipped with long neck funnel（72）, the long neck funnel（72）Neck mouth at be equipped with leakage valve（73）.

5. a kind of experiment nucleic acid extracting instrument according to claim 4, it is characterised in that：The long neck funnel（72）'s Outer surface is equipped with circular heating tube（74）.

6. a kind of experiment nucleic acid extracting instrument according to claim 1, it is characterised in that：The purification process platform（3） On through-hole（5）With cracking operation platform（4）On through-hole（5）Dislocation arrangement.