CN207516115U - A kind of biological cover plate for immunohistochemistry analyte detection cooperation slide - Google Patents

Abstract

The utility model is related to a kind of biological cover plates for immunohistochemistry analyte detection cooperation slide, there are one ontologies for tool, one recess portion with slide cooperation is formed at the lower part of the ontology, when being located in microscope slide on piece, the recess portion surrounding structure is fitted closely with micro slide upper surface, forms a reservoir compartment；A liquid feeding portion is further included, the liquid feeding portion is connected with reservoir compartment；Gas vent is further included in the ontology, the gas vent is located at the other end of the reservoir compartment relative to liquid feeding portion；The reservoir compartment is only connected by liquid feeding portion, gas vent with outside.The utility model can save testing cost, provides better observing effect and improve the efficiency of detection.

Description

A kind of biological cover plate for immunohistochemistry analyte detection cooperation slide

Technical field

The invention belongs to medical instruments fields, are specifically related to a kind of for immunohistochemistry analyte detection cooperation microscope slide The biological cover plate of piece.

Background technology

It is well-known for the processing method and the instrument that automatically processes of biological sample (such as anatomic pathologic sample) 's.This processing method may include the dye of those typical types in immunochemistry, in situ hybridization, specific stain and cytology Color process.In traditional experiment method, operating personnel are detected biological sample using immunohistochemical method, need It undergoes at least 60 multiple working procedures, spends more hours of at least five, the time is long, program is cumbersome.At present, domestic and international various big hospital And the equipment of the automatic detection instrument such as BOND-MAX models of German Lycra company production that testing agency, laboratory use, BenchMark XT immunohistochemistry autostainers etc. of Roche Holding Ag of Switzerland production, using detection device by above-mentioned operation from Dynamicization greatly improves the speed that pathology test can be completed, so as to cause diagnosis earlier, and in certain situations Under, result in intervention earlier.Dyeing is performed on sample usually on microscopic slide is placed in so that protrusion shows life Certain histologic characteristics in object sample.

But in some cases, dyeing, hybridization that robot is realized etc. is unbalanced or insecure, pathology Family therefore can not receive or the result using detection.The reason of detection failure, may be bubble in reagent (reaction solution), come from In the residue of reagent or other, in this case, the significant wastage to instrument and reagent will be caused.It discloses in the prior art A kind of slide lid (CN100342973C) of the storage tank with one, provides the lid for slide Son, include formed a cavity ontology, for be placed on microscope slide on piece formed a reaction chamber and from ontology one The protrusion of extension is held, a fluid tanks are formed between the leg on the both sides in protrusion and positioned at lid, work as lid The fluid tanks are connected with cavity fluid when son is loaded on slide.

In application process, reaction chamber is the space that sample is reacted with reaction solution, to be use up in this space Amount avoids the generation of gas, and needs the dosage of appropriate reduction reacting fluid, therefore, anti-by being added in fluid tanks one end Liquid is answered, lid is at certain angle of inclination with the plane being bonded of micro slide, utilizes the gravity of fluid and narrow space pair In the siphonage of liquid, reaction solution is made to enter reaction chamber, and drive gas therein out of reaction chamber, the use of reaction solution Typically about 100-200 microlitres of amount, the volume of this reaction chamber is about 110 microlitres, is reacted entire reaction chamber Liquid is full of.Since the thickness of reaction chamber is very thin, if reaction chamber is only connected by fluid tanks with outside, reaction solution It will be unable to enter reaction chamber, gas therein (air) can not at least be driven out of completely.It is opposite that the lid is utilized in lid In outflux of the other side as fluid of fluid tanks, reaction solution, will be in reaction chamber by the openning of this head piece Gas is driven out of, and using the tension of liquid, makes to keep being completely filled with by liquid and being unlikely to make reaction solution complete in reaction chamber It flows out in portion.

It will be appreciated, however, that in this way, this will lead to a unavoidable problem：1st, in order to ensure liquid Full of reaction chamber, the volume that reaction chamber will be substantially exceeded by the amount of the reaction solution of fluid tanks addition, therefore in the skill The container for having fluid tanks in technology disclosed in art scheme is more than the 50% of reaction chamber volume, and is reacted in actual mechanical process The demand of liquid is also more.Meanwhile do not stop to add in reaction solution, reaction solution also will ceaselessly be flowed by the openning of outflow Go out, this significant wastage that will cause reaction solution.2nd, during due to liquid feeding, enter the reaction solution of reaction chamber early period with sample Through being contacted and having been reacted, reaction effect is produced, but because reaction solution does not stop to add in, enter the anti-of reaction chamber early period Answer liquid that will be discharged reaction chamber, the result of this partial reaction liquid and example reaction will be unable to be observed after the completion of reaction, I.e. reaction effect will be diluted because of largely using for reaction solution, is influenced the visual observation to reaction result, can be caused to detect As a result there is the deviation of bigger.3rd, since the contact area of the reaction chamber in lid in the prior art and the external world is larger, Evaporation capacity is also big.It particularly needs to heat during reaction solution and example reaction, liquid is because of expanded by heating at this time, and evaporation capacity is more Greatly, in order to reduce evaporation, the anti-boiling substance of large dosage must be added in reaction solution in the prior art.But with anti-boiling substance Proportion increases, and the active ingredient proportion in reaction solution reduces, and the effect of reaction solution and example reaction is impacted.

Invention content

For reaction solution dosage in the prior art is big, the form and aspect after reaction solution and example reaction easily water down and Anti-boiling substance dosage it is big influence reaction effect the problem of, the present invention by changing disengaging mode of the reaction solution in reaction chamber, Achieve the purpose that save reaction solution, keep reaction condition and reduce anti-boiling substance accounting.It specifically provides a kind of for immune group The biological cover plate of compound detection cooperation slide, tool there are one ontology, the lower part of the ontology formed one with it is micro- The recess portion of mirror micro slide cooperation, when being located in microscope slide on piece, the recess portion surrounding structure and micro slide upper surface are close Fitting forms a reservoir compartment；A liquid feeding portion is further included, the liquid feeding portion is connected with reservoir compartment；The row of further including in the ontology Stomata, the gas vent are located at the other end of the reservoir compartment relative to liquid feeding portion；The reservoir compartment only by liquid feeding portion, gas vent with Outside connection.

Recess portion on ontology, and a kind of regular shape is not limited to, section both can be rectangle or circle Shape, ellipse, even other irregular shapes, but need meet detection biological sample minimum requirements, i.e., cover plate with it is micro- After the fitting of mirror micro slide, the reservoir compartment of formation should meet the needs of detection, its general volume is 50-200 microlitres.

Further, should be preferably 0.1mm-0.2mm on thickness for the reservoir compartment of formation.In practical application, When cover plate is located on micro slide, overall structure has certain angle of inclination, generally 5 ° -10 ° or so, can make so anti- Liquid is answered to enter reservoir compartment under gravity, and the air in reservoir compartment is driven out of.Certainly, if lid and micro slide Fitting plane does not have angle of inclination, by siphonage and liquid can be made to enter reservoir compartment.

With the entrance of reaction solution, the gas (substantially air) in reservoir compartment is extruded from gas vent.Gas vent Declaration of will in ontology is：Gas vent is the hole opened up on ontology, not in which marginal position of ontology, It should be through ontology, and the shape in section can be a variety of, it is most important that need the function of reaching exhaust, simultaneously It, should not be jagged at least on the section of the portion of gas vent.Further, gas vent can have multiple, but at least 1 A gas vent is located at the top position of ontology recess portion, and such purpose is that, can be with when adding in reaction solution into reservoir compartment Reaction solution is made to be full of entire reservoir compartment；Meanwhile if there is multiple gas vents, and it is in different height in use, that It is in the top position of the top position, not lower than reservoir compartment of that gas vent of lowest part, avoids so anti- Liquid is answered to be leaked out from the gas vent of lowest part.

Preferably, gas vent recited above, more high for ontology, i.e., gas vent is in the upper of ontology Portion forms a raised boss, entire gas vent such as form a hollow cylinder, the purpose done so is to prolong Length during long gas discharge.Because after reaction solution is injected, since it is desired that heated to substance to be measured, reaction solution will be with It heating and evaporates.By lengthening gas vent, evaporated vapour-phase reaction liquid can be made to be adhered to the inner wall of gas vent On, the loss of reaction solution is further reduced, meanwhile, reaction bench can also be avoided to overflow to a certain extent in this way.

In the present invention, when cover plate is located on micro slide, the gas in reservoir compartment is discharged by gas vent, but enters to storage Reaction solution in sap cavity, since reservoir compartment is only in communication with the outside by gas vent and liquid feeding portion, reaction solution is by gas in reservoir compartment After extrusion, the tension of liquid is utilized in vent ports, makes the liquid of filling opening can not add in, at this point, completely anti-in reservoir compartment Liquid is answered to be full of.At this point, since reaction solution can not go successively to reservoir compartment, will not be flowed out from reservoir compartment, it can be as needed Reaction solution is accurately added in into reservoir compartment, avoids the waste of reaction solution；Meanwhile in practical operation, into reservoir compartment Reaction solution is contacted with sample, and the effect of any a part of reaction solution and example reaction is maintained at reservoir compartment well In, without as a part of reaction solution loss for contacting with sample and reaction occurring in the prior art, causing to observe result It is inaccurate；Furthermore it is vented using gas vent, the contact area of reaction solution and external environment is small, the evaporation capacity in heating process It is small, in this case, it is possible to using the few reaction solution of anti-boiling substance accounting, can further intensified response effect, Improve the accuracy of detection.

Preferably, the liquid feeding portion includes complementary cavity and filling opening, when ontology is located in microscope slide on piece, institute It states recess portion and forms complementary cavity and reservoir compartment.This complementary cavity enters benefit closer to filling opening, reaction solution by filling opening Chamber is filled, enters back into reservoir compartment thereafter.Complementary cavity and reservoir compartment can be distinguished by forming different thickness, supplement The thickness of chamber is about between 0.5-2mm.

Further, the thickness of the complementary cavity is more than the reservoir compartment；When cover plate is located in microscope slide on piece, institute Reservoir compartment is stated only to connect with outside by the filling opening in the gas vent, liquid feeding portion.The thickness of complementary cavity is more than reservoir compartment, leads to It crosses filling opening and reaction solution is injected into complementary cavity, using the gravity of liquid itself, the reaction solution of complementary cavity is made to enter reservoir compartment It is contacted and is reacted with biological sample, and a certain amount of reaction solution is still stopped in complementary cavity.Because entire biological sample Detection process complex procedures, reaction solution is needed repeatedly to add in and flow out, while the process having, reaction solution terminate with example reaction Afterwards, it is also necessary to sample and reservoir compartment are rinsed using reaction solution.After reaction solution and example reaction, cover plate is dragged, is made Reaction solution flows out, at this point, the reaction solution stopped in complementary cavity can enter reservoir compartment, sample is rinsed, in next step Addition reaction solution carries out reaction and gets ready.By designing complementary cavity, it is possible to reduce the process of reaction solution injection improves work Make efficiency.

Preferably, when cover plate is located in microscope slide on piece, the filling opening extends outwardly from the complementary cavity, In the ontology.The position selection of filling opening mainly needs to realize toward reservoir compartment injection liquid or past complementary cavity Middle injection liquid, then the reaction solution in complementary cavity enter back into reservoir compartment.The design of filling opening can realize the fast of reaction solution Speed enters reservoir compartment, improves the efficiency of detection.Particularly in the case of the recess portion is divided into reservoir compartment and complementary cavity, Quickly reaction solution can be added in complementary cavity according to required amount is reacted.At this point, reaction solution is entered again by complementary cavity Reservoir compartment.There are two benefits in this way：When it is precisely quantitative, it is needed to add in reaction solution from filling opening according to detection, with the prior art It compares, has saved reaction solution, reduce testing cost；Second is that accelerating the speed of reaction solution addition, detection efficiency is improved.

Further, when cover plate is located in microscope slide on piece, the recess portion is with slide junction far from institute The one end for stating reservoir compartment forms notch, and the notch surrounds the filling opening with the slide.This is the present invention A kind of deformation, Basic Design thinking remains unchanged, while reservoir compartment and complementary cavity are still segmented at recess portion, passes through this The filling opening that a notch is formed with micro slide adds in liquid to complementary cavity.

Further, the liquid feeding portion is the protrusion body upper surface and the liquid filling hole with certain altitude.This is a kind of The mode of deformation, i.e. liquid feeding portion are only to protrude the liquid filling hole of body upper surface, and the volume of this liquid filling hole is more than needed for detection instead Answer liquid dosage 200 microlitres using the gravity of liquid, make reaction solution enter reservoir compartment, and by the gas in reservoir compartment from row Stomata is driven out of.Design in this way it is simple to operation, if the volume of liquid filling hole is met the requirements, and ensure heating process in sample place Position be immersed in reaction solution, it is possible to the height of liquid filling hole according to the size design of external equipment.

Preferably, the ontology extends in one end of the gas vent, further includes to connect with external mechanical structure Connecting portion.Cover plate of the present invention, it is not excluded that the detection of sample is carried out using the mode of manual operation, as long as operating When testing staff still can realize testing goal by manual methods.And connecting portion is set in one end of cover plate, it contributes to Using a kind of mode of mechanical structure control cover plate action.Since biology sample detection needs up to tens procedures, existing skill Mechanical structure may be used in art, reservoir compartment enters reaction solution by computer program realization and discharge the intelligence of reservoir compartment Control.Such as during detector of the carrier of cover plate utilization for certain existing brands, cover plate is connected to instrument by connecting portion It is acted in mechanical structure.Its act general flow be：Cover plate is placed on shelf, by a pulling device by cover plate in glass On piece drags, and achievees the purpose that dyeing and drain.

On the other hand, biological sample is placed on micro slide, cover plate is located in the position of micro slide, reaction solution and sample Reaction needs to discharge later, and the wind turbine in instrument will generate certain negative pressure in reservoir compartment at this time, enable reaction solution more thorough The discharge at bottom.Since the reaction solution that step different during sample detection uses is different, it is possible to reduce between differential responses liquid Interference.

Preferably, it is further included in ontology relative to the other end of both sides of the connecting portion with guiding role Guide rod.The effect of guide rod be by cover plate holding in the fixing device of instrument, ontology is made to be preferably located on micro slide, and According to the requirement of detection process, slided on scheduled track.Meanwhile also have and determined in different actions using cover plate Position, makes the position of cover plate disclosure satisfy that the requirement of detection.

Preferably, the surface of the recess portion has the coating that do not get wet.The effect of coating of not getting wet is so that reaction solution exists When discharge, under negative pressure and the collective effect of gravity, the reaction solution after reaction is discharged more clean thorough.It is this The coating that do not get wet can be silica or other have the material of this effect.

The present invention is to be compared with the advantages of the prior art：

First, during reaction solution is added in, be not in the situation that reaction solution spill-over comes out, realize detection process Being quantitatively adding for middle reaction solution, has saved testing cost.

Second is that in the step of each addition reaction solution, the reaction solution contacted with sample is held in reservoir compartment, sample The result that product and reaction solution reflect can all maintain, and be not in the situation that reaction result is watered down, detection is made all to be easier Observe detection result.

It should be noted that immunohistochemistry key step is divided into, piece is baked, is dewaxed, is repaired, dyeing and etc., wherein roasting piece It not needing to use reaction solution, remaining step is required to use reaction solution, and among these, the reaction solution that staining procedure uses Costly.

By taking the cover plate of Lycra company production used currently on the market as an example, the cover plate of Lycra adds in value height on machine Reaction solution (common first names：Antibody) it is divided into two different capacity, one is 100 microlitres, and one is 150 microlitres, just because of valency Value is high, thus while liquid consumption is reduced without make effect reduction be then it is particularly important, wherein the capacity of liquid and Cover plate is related with respect to the volume that slide is formed, and the cover plate volume of Lycra is 110 microlitres or so, if initial position adds in liquid again Body, then the liquid capacity needed considerably beyond 100 microlitres, so, 100 microlitres of positions are in slide in the cover plate of Lycra Between, this is to the more demanding of sample to be tested, it is necessary to which otherwise the lower part in cover plate can not be dyed, also mean that result It will appear deviation, this does not allow.Secondly, in liquid feeding there is dilute reaction solution in the cover plate of Lycra, that is to say, that When antibody is added in, there are antibody active ingredients to be diluted for Lycra cover plate, this is dense in other words for the dosage of reaction solution Degree has higher requirement, and also meaning that, which needs to pay higher cost, obtains testing more clearly observing effect.

So in order to solve these problems, we are subtracted in the design of cover plate using closure designs, the volume of middle chamber It is few, and realize the covering of sample, reduce the possibility of sample exposure.On the problem of dilution, we are used drain, are subtracted Few dilution allows active ingredient fully to obtain and is contacted and reacted with sample, provides better observing effect.

Third, the anti-boiling substance in reaction solution reduces dosage so that the active ingredient in reaction solution increases, and preferably increases Strong reaction effect；Fourth, the form in liquid feeding portion is more flexible, disposable addition reaction solution can be realized, thereafter again by reaction The gravity of liquid itself, makes reaction solution enter reservoir compartment, improves the efficiency of detection.

Description of the drawings

In order to illustrate more clearly of the present invention, will simply be situated between to attached drawing needed in embodiment description below It continues.It should be evident that the accompanying drawings in the following description is only the description to the embodiment of the present invention, for ordinary skill For personnel, without having to pay creative labor, can also simple deformation or title be carried out according to technical solution Change or take customary means, can also realize goal of the invention.

Fig. 1 is the stereogram of biological cover plate described in embodiment 1

Fig. 2 is the vertical view of biological cover plate described in embodiment 1

Fig. 3 is the front view of biological cover plate described in embodiment 1

Fig. 4 is the bottom view of biological cover plate described in embodiment 1

Fig. 5 is the bottom view of biological cover plate described in embodiment 1

Fig. 6 is schematic diagram when biological cover plate described in embodiment 1 is located in slide

Fig. 7 is position of the biological cover plate when reaction solution is discharged described in embodiment 1

Fig. 8 is the stereogram of biological cover plate described in embodiment 2

Fig. 9 is stereogram when biological cover plate described in embodiment 3 is located in micro slide

Figure 10 is the stereogram of biological cover plate described in embodiment 4

Figure 11 is the partial schematic diagram of biological cover plate described in embodiment 5

Specific embodiment

With reference to embodiment, the present invention is described in further detail, following embodiment be explanation of the invention and The invention is not limited in following embodiments.

Embodiment 1.

As shown in fig. 1 to 4, the present embodiment is discussed in detail a kind of for immunohistochemistry analyte detection cooperation slide Biological cover plate, cover plate tool have one piece of substrate 1, the lower part surrounding of substrate is four square ribs 2, and formed there are one ontology on ontology One rectangular slot, the rectangular slot in the present embodiment, is equivalent to aforementioned recess portion.The specification of rectangular slot be 46mm × 20.6mm, the face that four square ribs on substrate contacts with micro slide are in conplane, to ensure rectangular slot and load Object piece fits closely, and when cover plate is located on slide 3, the square rib 2 on cover plate forms a storage with micro slide 3 Sap cavity 4 and a complementary cavity 5, reservoir compartment 4 and complementary cavity 5 are a parts for rectangular slot, are divided in cover plate and micro slide 3 It is shown during from state.The body thickness of complementary cavity 5 is 1mm, and the thickness of reservoir compartment 4 is 0.12mm, as shown in figure 5, additional cavity Room 5 is separated with reservoir compartment 4 by a slope 6.A rounded corner is made in this slope, is conducive to reaction solution entrance in this way Reservoir compartment, while can prevent reservoir compartment from the problem of reaction solution adherency occur with complementary cavity's joining place.Entire complementary cavity Volume is slightly larger than 200 microlitres.

In the substrate, there are one filling opening 7, filling opening runs through substrate, is connected with complementary cavity, filling opening and complementary cavity Together constitute the liquid feeding portion in invention content.A gas vent 8 is further included in the substrate, and it is opposite that gas vent 8 is located at reservoir compartment In the other end of complementary cavity 5, specific is close to the rib 21 square over there far from complementary cavity；From figure it will be seen that Reservoir compartment 4 is only connected by the filling opening 7, the gas vent 8 that are connected with complementary cavity 5 with outside.

The present embodiment further includes two sections of guide rods 9, and guide rod is located at one end of substrate 1, in the present embodiment positioned at additional cavity That one end of room extends from substrate position to the both sides of substantially parallel substrate.Both ends guide rod is caught at instrument fixed position, makes lid Piece is acted along the track of limiting.In the present embodiment, in reservoir compartment one end of cover plate, ontology extended segment has further included one A connecting portion 10, this connecting portion are attached in the external mechanical structure of instrument, and external mechanical structure can be according to each step Suddenly the action implemented is needed, according to the preset program of instrument, the actions such as pulling in a series, playback are carried out to cover plate.Cover plate is determined When on micro slide, overall structure has 5 ° of angle of inclination for position.

For the present embodiment, most preferred scheme is that ontology, guide rod 9 and connecting portion 10 are to be integrally formed, wherein on ontology Four square ribs 2, substrates 1 be also to be integrally formed, such structural stability is good.

A certain kind can be realized for the instrument of biology sample detection, using the cover plate in the present embodiment, is carried out The step of sample detection, is substantially：1st, biological sample to be detected is placed on microscope slide on piece；2nd, micro slide is placed In the designated position of instrument；3rd, cover plate is limited at the designated position of instrument；4th, according to preset program, cover plate is positioned in loading On piece, cover plate and micro slide form reservoir compartment, complementary cavity at this time, it is notable that cover plate is contacted with micro slide at this time Plane, there is certain angle of inclination, and the region residing for sample, be in the position of reservoir compartment；5th, instrument is according to default Program is rapidly added about 200 microlitres of reaction solutions by filling opening to complementary cavity；6th, reaction solution is through complementary cavity, gravity with And under the action of siphonage, reservoir compartment is entered to, is contacted and is reacted with sample；7th, the gas in reservoir compartment is gradually anti- Liquid is answered to squeeze out, entire cavity is full of by reaction solution；8th, instrument is begun to warm up, and reaction solution is heated to be reacted with sample；9th, it reacts Terminate, observe and record reaction result at this time；10th, instrument pulls out cover plate, reaction solution according to default step by connecting portion Outflow at this time because instrument has XX components in itself, enhances the negative pressure of cover plate reservoir compartment, allows reaction solution more neatly Outflow；There is the reaction solution retained meanwhile in certain steps, in complementary cavity, sample surfaces can be rinsed；11、 By the reply of connecting portion, cover plate returns to origin-location, repeats and is acted next time.

Certainly, the object that the present embodiment uses be for certain instruments that can be applicable in, and if you do not need to instrument, for If each different links of biology sample detection carry out manual operation, guide rod, connecting portion are unnecessary in the present embodiment.That is just It is to say, it is only necessary to plus some additional operations, such as, correspond to the micro slide using object on ontology or in cover plate One tightening device of upper design, makes cover plate be fixed on micro slide, by manually carrying out heating etc. and feasible.

A variety of reaction solutions can be used in the present embodiment, for example existing frequently-used have dewaxing liquid, cleaning solution, repair liquid, wine The liquid such as essence.In the step of some need heating, anti-boiling substance (such as glycerine) content is low in used reaction solution, with sample The active ingredient that product can be reacted is high, and the effect of the reaction preferably improved has saved cost.

Embodiment 2

As shown in figure 8, the present embodiment and the difference of embodiment 1 are that rectangular slot is not divided into reservoir compartment and benefit Fill chamber, and only reservoir compartment.The contact surface that cover plate is located in micro slide still has certain gradient.Liquid feeding portion is through One liquid feeding column 11 of substrate, height 2mm, cross section are circular ring shape, and the volume of liquid feeding column 11 is 50 microlitres, liquid feeding column Shape and specification be by with the use of instrument limitation.When reaction solution is added in into reservoir compartment 4, reaction solution is in gravity Under effect, contacted and reacted with sample into reservoir compartment 4.The aperture of gas vent is smaller, anti-due to being added in reaction solution Substance is boiled, reaction solution is respectively provided with certain tension either under normal temperature state or under heated condition, can prevent reaction solution It is overflowed from gas vent.Certainly, in certain links, since the reaction solution in liquid feeding column 11 is driven out of by gas in reservoir compartment completely Afterwards, wherein being still stored with a certain amount of reaction solution, for flushing of the later stage to feature sample, and the height that these liquid are kept, It will not make to go successively under the effect of gravity, and the liquid of the inside is driven out of from gas vent.Based on this reason, the present embodiment In, the practical height of liquid feeding column is 2mm, and the aperture of gas vent is 1mm, and cover plate is located in the inclination angle of the contact surface of micro slide Spend is 5 °.

Embodiment 3

As shown in figure 9, the present embodiment is with embodiment 1, the difference lies in the position of filling opening is different.In embodiment 1 Filling opening be located on substrate, and in the present embodiment, filling opening 7 is such：Relative to the other end of reservoir compartment on ontology Square rib 21, for tool there are one scarf, this scarf to form filling opening 7 when cover plate is located on micro slide 3. On this filling opening 7, rounded corner can also be formed, convenient for reaction solution smoothly enter complementary cavity, thereafter by complementary cavity into Enter reservoir compartment.

Certainly, also another further design, i.e., the both ends shape that filling opening 7 is just have a certain degree, lack A kind of flared shape is formed at mouthful.The filling opening designed in this way is easier to realize in industrial production.

Embodiment 4

As shown in Figure 10, the difference lies in there are two gas vent tools, leans on embodiment illustrated in fig. 93 for the present embodiment The nearly square rib far from filling opening through substrate, makes reservoir compartment pass through two gas vents and is connected with outside.Be conducive to accelerate storage in this way Gas inside sap cavity discharges.

Embodiment 5

As shown in figure 11, the present embodiment and the difference of embodiment 1 are that gas vent 8 forms one in the substrate of ontology The cylindrical boss of a middle part transmission.The effect of this boss is that the exhaust of the vaporization of reaction solution in heating process can be extended Length, makes the reaction solution of vaporization can be adhered on the inner wall of gas vent, reduces the loss of reaction solution；Furthermore it is also possible to effectively Reaction solution is avoided to be overflowed in injection process.

Embodiment 6

The present embodiment and the difference of embodiment 1 are there is the one layer of coating that do not get wet in the rectangular slot in embodiment, This coating is silica.Inside is coated with the one layer of coating that do not get wet, and can reduce reaction solution and the adherency on the inside of rectangular slot, Improve reaction solution entrance and discharge complementary cavity, the efficiency of reservoir compartment.

In the present invention, we are using the cover plate in the original-pack cover plate of Lycra, embodiment 1 and the cover plate in embodiment 5, to biology Sample is detected, and forms a correction data.

The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art Member, without departing from the inventive concept of the premise, can also make several improvements and modifications, these improvements and modifications also should be regarded as In protection scope of the present invention.Although the present invention is disclosed as above with embodiment, it is not limited to the protection of the present invention Range, any technical staff for being familiar with this technology, in the variation and retouching made without departing from the spirit and scope of the invention, It is within the scope of protection of the invention.

Claims (10)

1. a kind of biological cover plate for immunohistochemistry analyte detection cooperation slide, which is characterized in that there are one this for tool Body, a recess portion with slide cooperation is formed at the lower part of the ontology, described when being located in microscope slide on piece Recess portion surrounding structure is fitted closely with micro slide upper surface, forms a reservoir compartment；

A liquid feeding portion is further included, the liquid feeding portion is connected with reservoir compartment；

Gas vent is further included in the ontology, the gas vent is located at the other end of the reservoir compartment relative to liquid feeding portion；

The reservoir compartment is only connected by liquid feeding portion, gas vent with outside.

2. a kind of biological cover plate for immunohistochemistry analyte detection cooperation slide as described in claim 1, special Sign is, when cover plate is located in microscope slide on piece, the gas vent at least described in 1 is located at the top position of the recess portion It puts.

3. a kind of biological cover plate for immunohistochemistry analyte detection cooperation slide as claimed in claim 2, special Sign is that the gas vent runs through the ontology, and the upper surface of the gas vent is higher than the upper surface of the ontology, the exhaust Hole forms a boss on the body.

4. a kind of life for immunohistochemistry analyte detection cooperation slide as described in claims 1 to 3 any one Object cover plate, which is characterized in that the liquid feeding portion includes complementary cavity and filling opening, and the ontology is located in microscope slide on piece When, the recess portion forms complementary cavity and reservoir compartment.

5. a kind of biological cover plate for immunohistochemistry analyte detection cooperation slide as claimed in claim 4, special Sign is that the thickness of the complementary cavity is more than the reservoir compartment；When cover plate is located in microscope slide on piece, the reservoir compartment Only connected by the filling opening in the gas vent, liquid feeding portion with outside.

6. a kind of biological cover plate for immunohistochemistry analyte detection cooperation slide as claimed in claim 5, special Sign is, when cover plate is located in microscope slide on piece, the filling opening extends outwardly from the complementary cavity, positioned at described In body.

7. a kind of biological cover plate for immunohistochemistry analyte detection cooperation slide as claimed in claim 5, special Sign is, when cover plate is located in microscope slide on piece, the recess portion is with slide junction far from the reservoir compartment One end form notch, the notch and the slide surround the filling opening.

8. a kind of life for immunohistochemistry analyte detection cooperation slide as described in claims 1 to 3 any one Object cover plate, which is characterized in that the liquid feeding portion is the liquid filling hole for protruding the body upper surface.

9. a kind of life for immunohistochemistry analyte detection cooperation slide as described in claims 1 to 3 any one Object cover plate, which is characterized in that the ontology close to the gas vent one end extend, further include for external mechanical structure The connecting portion of connection and/or in ontology relative to the other end of both sides of the connecting portion, further include with guiding role Guide rod.

10. a kind of life for immunohistochemistry analyte detection cooperation slide as described in claims 1 to 3 any one Object cover plate, which is characterized in that the surface of the recess portion has the coating that do not get wet.