CN207248896U - Dog c reactive protein time-resolved fluoroimmunoassay chromatographs detection kit - Google Patents
Dog c reactive protein time-resolved fluoroimmunoassay chromatographs detection kit Download PDFInfo
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- CN207248896U CN207248896U CN201721371201.8U CN201721371201U CN207248896U CN 207248896 U CN207248896 U CN 207248896U CN 201721371201 U CN201721371201 U CN 201721371201U CN 207248896 U CN207248896 U CN 207248896U
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Abstract
Dog c reactive protein time-resolved fluoroimmunoassay chromatographs detection kit, including box body, buffer solution bottle is embedded with box body, suction nozzle and test card, one end of test card is handle end, the other end of test card is information terminal, information terminal is equipped with character layer, test card includes cover board, test strips and bottom plate, the outer wall of cover board is equipped with the barcode scanning layer of recognition detection object, test strips include the connected sample pad of overlap joint, labeling pad, detecting pad and water absorption pad, the anti-dog c reactive protein antibody one of mouse of time-resolved fluorescence microballoon mark is coated with labeling pad, detection line and nature controlling line are installed with detecting pad, detection line is coated with the anti-dog c reactive protein antibody two of mouse, nature controlling line is coated with sheep anti mouse secondary antibody；Cover board offers loading slot and detect tank, and the loading slot of cover board towards sample pad and surrounds out sample-adding portion, and sample-adding portion is close to handle end；The detect tank of cover board is towards detecting pad and surrounds out test section, and test section is located in test section close to information terminal, detection line and nature controlling line.
Description
Technical field
The utility model belongs to biological technical field, is related to a kind of dog c reactive protein time-resolved fluoroimmunoassay chromatography inspection
Test agent box.
Background technology
C reactive protein (CRP) is the constituent of body non-immunological systems, is had in the acute stage of a variety of mammalian diseases
Important diagnostic alerts effect, the concentration of CRP are not influenced be subject to antibiotic and immunodepressant, in the treatment can be exactly
Development to disease is monitored, and has very high value in clinic diagnosis.The detection of dog CRP is in veterinary clinic application
Than wide, include monitoring, the sight of antibiotic curative effect of the diagnosis and differential diagnosis, post-operative infection of acute infectious diseases
Examine, course of disease monitoring and Index for diagnosis etc..Dog CRP is high to disease susceptibility, can be in the quick rise of disease early stage, timely reflection disease
Feelings；Therefore Inflammation Marker is used as, effect increasingly significants of the dog CRP on veterinary clinic, detection dog CRP is to clinical diagnosis, treatment
Effect and Observation On The Prognosis etc. have important value.
Research shows that people and dog CRP do not have common antigenicity, therefore the quick determination method of dog CRP cannot rely upon
Human C-reactiveprotein Rapid detection test strip.At present, the method for quick detection dog CRP is to rely on clinical detection instrument more in clinical, this
Average pet hospital does not have.Moreover, diagnosing industry for animal epidemic, in many cases, the diagnosis for disease needs
Carry out in the wild, existing dog CRP detection methods are difficult to complete.At present, it is difficult to simple operation dog CRP is carried out quick
Detection, and detect it is low there are sensitivity, can only qualitative or sxemiquantitative the problem of, this, which is that those skilled in the art are urgently to be resolved hurrily, asks
Topic.
Utility model content
The purpose of this utility model is that there are the above problem for existing technology, it is proposed that during a kind of dog c reactive protein
Between resolved fluorometric immunochromatographytest test kit, be used for realization the quick detection of dog c reactive protein.
The purpose of this utility model can be realized by following technical proposal：
Dog c reactive protein time-resolved fluoroimmunoassay chromatographs detection kit, including box body, and buffer solution is embedded with box body
Bottle, suction nozzle and test card, one end of test card is handle end, and the other end of test card is information terminal, and information terminal is equipped with word
Layer, test card include cover board, test strips and bottom plate, and test strips are fixed between cover board and bottom plate, and the outer wall of cover board, which is equipped with, to be known
The barcode scanning layer of other detection object, test strips include connected sample pad, labeling pad, detecting pad and the water absorption pad of overlap joint；Label
The anti-dog c reactive protein antibody one of mouse of time-resolved fluorescence microballoon mark is coated with pad, be installed with detecting pad detection line and
Nature controlling line, nature controlling line are adjacent to water absorption pad, and detection line is coated with the anti-dog c reactive protein antibody two of mouse, and nature controlling line is coated with goat-anti
Mouse secondary antibody；Cover board offers loading slot and detect tank, and the loading slot of cover board towards sample pad and surrounds out sample-adding portion, and sample-adding portion is leaned on
It is bordering on handle end；The detect tank of cover board is towards detecting pad and surrounds out test section, and test section is close to information terminal, detection line and matter
Control line is located in test section.
In above-mentioned dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit, sample pad is glass fibre,
Filter paper or hemofiltration film；Labeling pad is glass fibre, filter paper or polyester film；Detecting pad is nitrocellulose filter or acetic acid
Cellulose membrane；Water absorption pad is blotting paper.In above-mentioned dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit,
Either Quick Response Code barcode scanning layer is cohesive or is printed between loading slot and detect tank for bar code for barcode scanning layer.
In above-mentioned dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit, the outer wall of bottom plate offers
Sliding slot, the depth of sliding slot are less than the thickness of bottom plate, and sliding slot extends to handle end along the central axes of bottom plate from information terminal.
In above-mentioned dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit, one end of sampling rod is cotton
Caput, the other end of sampling rod is fluid suction head.
Compared with prior art, the technical program has the advantage that：
1st, reagent cartridge configuration is simple, easy to operation, during detection, sample liquid is added drop-wise to after being reacted 15 minutes on test card, just
It can be detected as a result, detection time is fast, without relying on complicated detecting instrument, be not only restricted to detection occasion；Barcode scanning layer
Setting improve the speed of quantitative detection, it is not necessary to setting detection object is artificially gone to fluorescence detector, saves operation
Time, it is therefore prevented that the situation generation of error and standard curve download mistake is manually set, it is ensured that the accuracy of detection and analysis, it is real
Now quickly quantitative detection.
2nd, sliding slot plays position-limiting action, defines the insertion depth of test card, in order to operate, during quantitative analysis, in cunning
Groove it is spacing under, handle end is in outside socket, and sample-adding portion is in socket, so that it is outer to avoid in quantitative analysis sample-adding portion from being subject to
Pollute on boundary, it is ensured that the accuracy of detection.
Brief description of the drawings
Fig. 1 is the structural representation of the dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit of the utility model
Figure.
Fig. 2 is the test card of the dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit of the utility model
Front schematic view.
Fig. 3 is the test card of the dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit of the utility model
Schematic rear view.
Fig. 4 is the test card of the dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit of the utility model
Sectional view.
In figure, 1, box body；2nd, test card；3rd, cover board；4th, test strips；5th, bottom plate；6th, sample pad；7th, labeling pad；8th, examine
Survey pad；9th, water absorption pad；10th, detection line；11st, nature controlling line；12nd, loading slot；13rd, detect tank；14th, sample-adding portion；15th, test section；16、
Character layer；17th, barcode scanning layer；18th, handle end；19th, information terminal；20th, buffer solution bottle；21st, suction nozzle；22nd, sampling rod；23rd, sampling bottle；
24th, sliding slot；25th, swab stick head；26th, fluid suction head.
Embodiment
It is specific embodiment of the utility model and with reference to attached drawing below, the technical solution of the utility model is made further
Description, but the utility model is not limited to these embodiments.
As shown in Figures 1 to 4, the utility model provides a kind of dog c reactive protein time-resolved fluoroimmunoassay chromatography detection
Kit, for the Quantitative detection of dog c reactive protein, including box body 1, is embedded with one bottle of buffer solution bottle 20, one in box body 1
Branch suction nozzle 21 and one piece of test card 2, box body 1 separate out the card slot of multiple and different shapes, buffer solution bottle 20, suction nozzle 21 and test card
2 are embedded in the corresponding card slot of shape therewith respectively, to avoid mutual collision.A operation instruction is also placed with box body 1
Book.
Sample is added into vibrated in buffer solution bottle 20 after form sample liquid, suction nozzle 21 be used for Aspirate supernatant and by its
It is added drop-wise on test card 2.Selectively, a sampling rod 22 and a sampling bottle 23 are also embedded with box body 1, sampling rod 22 carries
Take sample and transfer the sample into sampling bottle 23, buffer solution is then added into sampling bottle 23 vibrates to form sample liquid, then uses
Suction nozzle 21 draws the supernatant of sample liquid and is added dropwise to test card 2 and is detected.
One end of test card 2 is handle end 18, and when operation holds pommel 18 of stopping, and the other end of test card 2 is information terminal
19, information terminal 19 is equipped with the character layer 16 of display detection object, and character layer 16 has the name information on detection object, text
Word layer 16 confirms to be used for operator, and operator confirms whether the test card 2 is suitable for detection dog C protein by character layer 16,
Avoid malfunctioning.
Test card 2 includes cover board 3, test strips 4 and bottom plate 5, and test strips 4 are fixed between cover board 3 and bottom plate 5, test paper
Bar 4 is bonded in the inner wall of bottom plate 5, and the lid of cover board 3 is located in test strips 4 and connects with the engaging of bottom plate 5.The outer wall of cover board 3 is equipped with
The barcode scanning layer 17 of recognition detection object, barcode scanning layer 17 have the coding information on detection object.
During quantitative analysis, test card 2 coordinates fluorescence detector (existing instrument) to use, and sample liquid is added drop-wise in test strips 4,
Fluorescence signal is detected with fluorescence detector after reacting 15 minutes, the utility model focuses on kit, and fluorescence detector is not
Make specific expansion, test card 2 is inserted into the concrete numerical value information that dog c reactive protein just can be exported in fluorescence detector.
It is noted that barcode scanning layer 17 is identified detection object for fluorescence detector, fluorescence detector scanning
After barcode scanning layer 17 recognizes detection object, the standard curve for obtaining dog c reactive protein is downloaded, fluorescence detector detects that fluorescence is believed
The dog c reactive protein concentration in sample is calculated according to dog c reactive protein standard curve after number.The setting of barcode scanning layer 17 improves
The speed of quantitative detection, it is not necessary to setting detection object is artificially gone to fluorescence detector, saves the operating time, it is therefore prevented that
The situation generation of error and dog c reactive protein standard curve download mistake is manually set, it is ensured that the accuracy of detection and analysis.
Specifically, test strips 4 include connected sample pad 6, labeling pad 7, detecting pad 8 and the water absorption pad 9 of overlap joint, label
Pad 7 is between sample pad 6 and detecting pad 8, and detecting pad 8 is between labeling pad 7 and water absorption pad 9.
The anti-dog c reactive protein antibody one of mouse of time-resolved fluorescence microballoon mark, detecting pad 8 are coated with labeling pad 7
On be installed with detection line 10 and nature controlling line 11, detection line 10 is coated with the anti-dog c reactive protein antibody two of mouse, and nature controlling line 11 is coated with
Sheep anti mouse secondary antibody；Detection line 10 and nature controlling line 11 are parallel, and nature controlling line 11 is adjacent to water absorption pad 9.The anti-dog c reactive protein antibody of mouse
One and the anti-dog c reactive protein antibody two of mouse be two different epitopes the anti-dog c reactive protein antibody of mouse, i.e., using double-antibody sandwich
Method.
Cover board 3 offers loading slot 12 and detect tank 13, and the loading slot 12 of cover board 3 towards sample pad 6 and surrounds out sample-adding
Portion 14, sample-adding portion 14 is close to handle end 18；The detect tank 13 of cover board 3 is towards detecting pad 8 and surrounds out test section 15, test section
15 are located in test section 15 close to information terminal 19, detection line 10 and nature controlling line 11.In addition, the side of detect tank 13 is equipped with detection
Line 10 marks T and nature controlling line 11 marks C, and detection line 10 marks the detection line 10 of T direct detections pad 8, and nature controlling line 11 marks C to be directed toward
The nature controlling line 11 of detecting pad 8, easy to qualitatively judge, qualitative judgement can be visually observed directly using laser irradiating and detecting portion 15.
Further, the cell wall of loading slot 12 slopes inwardly, and the top edge size of loading slot 12 is more than its lower edge size,
Sample liquid can be formed and converged.The cell wall of detect tank 13 slopes inwardly, and the top edge size of detect tank 13 is more than its lower edge size,
To form the laser entry portal of big opening.Character layer 16 bond or be printed on cover board 3 upper end edge and detect tank 13 it
Between.
The material that bottom plate 5 and cover board 3 are PVC boards or other hard do not absorb water, sample pad 6 for glass fibre, filter paper or
Hemofiltration film；Labeling pad 7 is glass fibre, filter paper or polyester film, can be with when sample pad 6 is identical with the material of labeling pad 7
Combine；Detecting pad 8 is nitrocellulose filter or cellulose acetate film, preferably nitrocellulose filter；Water absorption pad 9 is
Blotting paper.
Selectively, for bar code, either Quick Response Code barcode scanning layer 17 bonds or is printed on loading slot 12 to barcode scanning layer 17
Between detect tank 13, to separate the reading of the collection of fluorescence signal and coding information.
Selectively, the outer wall of bottom plate 5 offers sliding slot 24, and the depth of sliding slot 24 is less than the thickness of bottom plate 5, and sliding slot 24 is not
Through the inner wall of bottom plate 5, the bottom surface of test strips 4 will not be revealed in sliding slot 24, so as to avoid test strips 4 from being contaminated.Sliding slot
24 length is less than the length of bottom plate 5, and sliding slot 24 extends to handle end along the inward flange of the central axes of bottom plate 5 from information terminal 19
There is spacing between the both ends of 18 inward flange, sliding slot 24 and bottom plate 5.
Sliding slot 24 plays position-limiting action when test card 2 is inserted into fluorescence detector, defines the insertion depth of test card 2, with
It is easy to operation.During quantitative analysis, hold live test card 2 handle end 18, by information terminal 19 towards fluorescence detector socket simultaneously
Insertion, sliding slot 24 it is spacing under, handle end 18 is in outside socket, and sample-adding portion 14 is in socket, so as to avoid quantitatively dividing
Sample-adding portion 14 is subject to outside contamination during analysis, it is ensured that the accuracy of detection.
Selectively, one end of sampling rod 22 is swab stick first 25, and the other end of sampling rod 22 is fluid suction head 26.Sampling rod 22
Solid and liquid can be extracted at the same time, and swab stick first 25 is used to extract solid fraction sample, and fluid suction head 26 is used for imbibition, easy to operation.
The quantitative testing principle of dog c reactive protein is：Supernatant is taken after sample to be tested is mixed with buffer solution, and by supernatant
Liquid after loading slot 12 is added drop-wise in the sample-adding portion 14 of test card 2, dog c reactive protein in sample with solution from sample pad 6 very
Fast chromatography arrives labeling pad 7, and dog c reactive protein and the anti-dog C of mouse that time-resolved fluorescence microballoon in labeling pad 7 marks are anti-at this time
Protein antibodies one are answered to form fluorescent microsphere-mouse anti-dog c reactive protein antibody one-dog c reactive protein compound, the compound is with molten
Liquid layer is analysed onto detecting pad 8, is combined when reaching detection line 10 (T lines) with the anti-dog c reactive protein antibody two of the mouse at this, is formed glimmering
Light microballoon-mouse one-dog of anti-dog c reactive protein antibody c reactive protein-anti-two compound of dog c reactive protein antibody of mouse is simultaneously fixed
In T lines, and first the excessive anti-dog c reactive protein antibody of fluorescent microsphere-mouse continues chromatography and arrives nature controlling line 11 (C lines), and herein
Sheep anti mouse secondary antibody formed fluorescent microsphere-mouse anti-dog c reactive protein antibody one-sheep anti mouse secondary antibody compound.After chromatographing 15 minutes,
With the test section 15 of exciting light irradiating and detecting pad 8, it will detect that T lines, C lines have fluorescence, according to standard curve and fluorescent value
Concentration to be measured can be calculated in ratio.
The specific embodiments described herein are merely examples of the spirit of the present invention.The utility model institute
Described specific embodiment can be done various modifications or additions or using similar by belonging to those skilled in the art
Mode substitute, but without departing from the spirit of the present application or beyond the scope of the appended claims.
Although box body 1 is used more herein；Test card 2；Cover board 3；Test strips 4；Bottom plate 5；Sample pad 6；Label
Pad 7；Detecting pad 8；Water absorption pad 9；Detection line 10；Nature controlling line 11；Loading slot 12；Detect tank 13；Sample-adding portion 14；Test section 15；Text
Word layer 16；Barcode scanning layer 17；Handle end 18；Information terminal 19；Buffer solution bottle 20；Suction nozzle 21；Sampling rod 22；Sampling bottle 23；Sliding slot 24；
Swab stick first 25；The grade term of fluid suction head 26, but it does not preclude the possibility of using other terms.The use of these items is only for
More easily describe and explain the essence of the utility model；Being construed as any one of the additional limitations is all and this practicality
What new spirit was disagreed.
Claims (5)
1. dog c reactive protein time-resolved fluoroimmunoassay chromatographs detection kit, including box body, it is characterised in that box body embeds
Equipped with buffer solution bottle, suction nozzle and test card, one end of test card is handle end, and the other end of test card is information terminal, information terminal
Character layer is equipped with, test card includes cover board, test strips and bottom plate, and test strips are fixed between cover board and bottom plate, cover board
Outer wall is equipped with the barcode scanning layer of recognition detection object, and test strips include connected sample pad, labeling pad, detecting pad and the water suction of overlap joint
Pad；The anti-dog c reactive protein antibody one of mouse of time-resolved fluorescence microballoon mark is coated with labeling pad, is installed with detecting pad
Detection line and nature controlling line, nature controlling line are adjacent to water absorption pad, and detection line is coated with the anti-dog c reactive protein antibody two of mouse, nature controlling line bag
There is sheep anti mouse secondary antibody；Cover board offers loading slot and detect tank, and the loading slot of cover board towards sample pad and surrounds out sample-adding portion,
Sample-adding portion is close to handle end；The detect tank of cover board is towards detecting pad and surrounds out test section, and test section is examined close to information terminal
Survey line and nature controlling line are located in test section.
2. dog c reactive protein time-resolved fluoroimmunoassay according to claim 1 chromatographs detection kit, its feature exists
In sample pad is glass fibre, filter paper or hemofiltration film；Labeling pad is glass fibre, filter paper or polyester film；Detecting pad is
Nitrocellulose filter or cellulose acetate film；Water absorption pad is blotting paper.
3. dog c reactive protein time-resolved fluoroimmunoassay according to claim 1 chromatographs detection kit, its feature exists
In either Quick Response Code barcode scanning layer is cohesive or is printed between loading slot and detect tank for bar code for barcode scanning layer.
4. dog c reactive protein time-resolved fluoroimmunoassay according to claim 1 chromatographs detection kit, its feature exists
Sliding slot is offered in the outer wall of, bottom plate, and the depth of sliding slot is less than the thickness of bottom plate, and sliding slot is along the central axes of bottom plate from information terminal
Extend to handle end.
5. dog c reactive protein time-resolved fluoroimmunoassay according to claim 1 chromatographs detection kit, its feature exists
In being also embedded with sampling rod in box body, one end of sampling rod is swab stick head, and the other end of sampling rod is fluid suction head.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201721371201.8U CN207248896U (en) | 2017-10-23 | 2017-10-23 | Dog c reactive protein time-resolved fluoroimmunoassay chromatographs detection kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721371201.8U CN207248896U (en) | 2017-10-23 | 2017-10-23 | Dog c reactive protein time-resolved fluoroimmunoassay chromatographs detection kit |
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| CN207248896U true CN207248896U (en) | 2018-04-17 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108918884A (en) * | 2018-06-08 | 2018-11-30 | 广州海孚医疗科技有限公司 | The immuno-chromatographic test paper strip and preparation method thereof of quantitative detection dog c reactive protein |
| CN109580937A (en) * | 2018-12-07 | 2019-04-05 | 武汉纽康度生物科技股份有限公司 | Full-automatic detection card |
| CN112285358A (en) * | 2020-09-17 | 2021-01-29 | 上海基灵生物科技有限公司 | Reagent card for dog C-reactive protein and pancreas specific lipase duplex detection |
| WO2023147713A1 (en) * | 2022-02-07 | 2023-08-10 | 嘉兴康源科泰科技发展有限公司 | Combined detection structure for dual sampling and integrated detection and use thereof |
-
2017
- 2017-10-23 CN CN201721371201.8U patent/CN207248896U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108918884A (en) * | 2018-06-08 | 2018-11-30 | 广州海孚医疗科技有限公司 | The immuno-chromatographic test paper strip and preparation method thereof of quantitative detection dog c reactive protein |
| CN109580937A (en) * | 2018-12-07 | 2019-04-05 | 武汉纽康度生物科技股份有限公司 | Full-automatic detection card |
| CN112285358A (en) * | 2020-09-17 | 2021-01-29 | 上海基灵生物科技有限公司 | Reagent card for dog C-reactive protein and pancreas specific lipase duplex detection |
| WO2023147713A1 (en) * | 2022-02-07 | 2023-08-10 | 嘉兴康源科泰科技发展有限公司 | Combined detection structure for dual sampling and integrated detection and use thereof |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Dog C reaction albumen time -resolved fluorescence immunity chromatography detect reagent box Effective date of registration: 20190709 Granted publication date: 20180417 Pledgee: Agricultural Bank of China Limited by Share Ltd Jiaxing Economic Development Zone sub branch Pledgor: JIAXING CHAOYUNFAN BIOTECHNOLOGY CO., LTD. Registration number: 2019330000184 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |