CN207175972U - The pH on-line measuring devices of nutrient solution in bioreactor - Google Patents
The pH on-line measuring devices of nutrient solution in bioreactor Download PDFInfo
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- CN207175972U CN207175972U CN201721006177.8U CN201721006177U CN207175972U CN 207175972 U CN207175972 U CN 207175972U CN 201721006177 U CN201721006177 U CN 201721006177U CN 207175972 U CN207175972 U CN 207175972U
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Abstract
The utility model discloses a kind of on-line measuring device of medium pH value in bioreactor, including：Bioreactor joint, input duct, detection cell and overflow pipe；Wherein, one end of the input duct penetrates with the detection cell, and the other end is connected with bioreactor joint；The overflow pipe connects with detection cell；PH value detection means is set in detection cell.The utility model device is directly connected by bioreactor joint with the sampling pump of bioreactor, nutrient solution sample in bioreactor can realize the pH detections of real-time online, medium pH value can truly be determined so as to facilitate the regulation and control of cell tank culture, the expression quantity of cytoactive and purpose product is improved, and then realizes volume increase.The utility model apparatus structure is simple, prepares the pH value that cost is low, can realize nutrient solution in quick, accurate, real-time high-efficiency detection bioreactor.
Description
Technical field
It the utility model is related to a kind of medium pH value detection means, more particularly to nutrient solution in bioreactor
PH on-line measuring devices, belong to pH on-line measuring devices field.
Background technology
The biological medicament of particularly eukaryotic cell expression is typically directed in bioreactor in biological medicament production
The pH detections of nutrient solution.General bioreactor has the pH value of oneself to detect and can be real-time display, but bioreactor is certainly
The pH detecting electrodes of band only before bioreactor sterilizes calibration once, production period for ensure in reactor it is sterile can not be again
It is secondary that pH electrodes are calibrated.So, bioreactor particularly flow plus perfusion culture reactor typically continuously run 1 week or
Person after 2 weeks pH detection can drift about deviate actual value.The method for solving this problem at present is nutrient solution in observing response device
Growth of the color by micro-judgment pH if appropriate for cell, another method are exactly offline inspection after being sampled by sampling pump
PH value；Above-mentioned both approaches can not grasp real pH value in cell reactor.
Be badly in need of developing at present it is a kind of being capable of nutrient solution in succinct, efficient, accurate on-line checking bioreactor
PH value detection means.
Utility model content
Technical problem to be solved in the utility model is to overcome existing detection method or detection means to grasp
In cell reactor the defects of real pH value, there is provided one kind being capable of succinct, efficient, accurate on-line checking cell biological reaction
The pH value detection means of nutrient solution in device.
In order to solve the above technical problems, technical solution adopted in the utility model is：
The on-line measuring device of medium pH value in a kind of bioreactor, including：Bioreactor joint,
Input duct, detection cell and overflow pipe；Wherein, one end of the input duct penetrates with the detection cell, the other end and biology
Reactor joint is connected；The overflow pipe connects with detection cell.
The sampling pump that described bioreactor joint can directly be connected bioreactor with cell is connected, because
This, the nutrient solution sample in bioreactor is passed through bioreactor joint and the input duct being connected with joint by sampling pump
It is pumped into detection cell, on-line checking is completed to the pH value of nutrient solution in detection cell, then be discharged to outside detection cell by overflow pipe；
Whole process of the nutrient solution in the detection means is all unidirectionally to flow, and can effectively ensure that the sterile of reactor.
As a kind of preferable structure of the utility model, the bottom of the overflow pipe and detection cell connects, with ensure into
The culture liquid energy entered in detection cell is smoothly discharged to outside detection cell by overflow pipe.
As another preferable structure of the utility model, pH value detection means, the pH can be placed in detection cell
Detection means can detect the various usual means of pH value, such as can be pH detectors, pH electrodes, pH detection probes, pH
Meter etc., preferably pH electrodes.
The main beneficial effect of the on-line measuring device of medium pH value includes in the utility model bioreactor：
The utility model device is joined directly together with being connected the sampling pump of bioreactor by joint and connect, the nutrient solution in bioreactor
Sample can realize the pH detections of real-time online, can truly determine the pH value of nutrient solution so as to facilitate the tune of cell tank culture
Control, the expression quantity of cytoactive and purpose product is improved, and then realize volume increase；The utility model apparatus structure is simple, is prepared into
Originally pH value low, that nutrient solution in quick, accurate, real-time high-efficiency detection bioreactor can be realized.
Brief description of the drawings
Fig. 1 is a specific implementation of the on-line measuring device of medium pH value in the utility model bioreactor
The structure chart of example；
Fig. 2 is that the on-line measuring device of medium pH value in the utility model bioreactor carries out on-line checking
Operating diagram.
Reference：1- bioreactor joints, 2- input ducts, 3- detection cells, 4-pH value detection devices (pH electricity
Pole), 5- overflow pipes, the sampling pump of 6- connection bioreactors, 7- bioreactors.
Embodiment
The utility model is further described with reference to specific accompanying drawing, will be with feature the advantages of the utility model
Description and it is apparent.It should be understood that the embodiment is only exemplary, the scope of the utility model is not formed any
Limitation.It will be understood by those skilled in the art that can be to this practicality under without departing from spirit and scope of the present utility model
The details and form of new technique scheme are modified or replaced, but these modifications or substitutions each fall within protection of the present utility model
Scope.
It is the utility model bioreactor on-line checking pH value apparatus structure schematic diagram referring to Fig. 1, including：Cell
Bioreactor joint 1, input duct 2, detection cell 3 and overflow pipe 5；Wherein, one end of the input duct 2 and the detection
Pond 3 penetrates, and the other end is connected with bioreactor joint 1；The overflow pipe 5 connects with detection cell 3.
As a kind of preferred embodiment of the utility model (not shown), the bottom of the overflow pipe 5 and detection cell 3 is mutually passed through
It is logical, to ensure that the culture liquid energy entered in detection cell 3 is smoothly discharged to outside detection cell 3 by overflow pipe 5.
As another preferred embodiment of the present utility model, pH value detection means 4 is set in detection cell 3, it is described
PH detection means 4 can be that to detect pH value can be pH detectors, pH electrodes, pH detection probes, pH meter etc., in this practicality newly
In one most preferred embodiment of type, described pH value detection means 4 is pH electrodes.
Fig. 2 is that the on-line measuring device of medium pH value in the utility model bioreactor carries out on-line checking
Operation principle schematic diagram, the bioreactor joint 1 of the utility model detection means is directly with being connected bioreactor
Sampling pump 6 is connected；Sampling pump 6 by the nutrient solution sample in bioreactor 7 by bioreactor joint 1,
Feed tube 2 is pumped into detection cell 3, completes on-line checking to medium pH value using pH electrodes 4 in detection cell 3, then pass through
Overflow pipe 5 is discharged to outside detection cell 3, and whole process of the nutrient solution in this on-line measuring device is all unidirectionally to flow, and can effectively be protected
Demonstrate,prove the sterile of bioreactor.
In the utility model, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " Gu
It is fixed " etc. term should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or integrally；It can be machine
Tool connects；Can be joined directly together, can also be indirectly connected by intermediary, can be the connection or two of two element internals
The interaction relationship of individual element.For the ordinary skill in the art, above-mentioned art can be understood as the case may be
Concrete meaning of the language in the utility model.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment or example of the present utility model.In this manual, to the schematic table of above-mentioned term
Identical embodiment or example need not be directed to by stating.Moreover, specific features, structure, material or the feature of description can be
Combined in an appropriate manner in any one or more embodiments or example.In addition, in the case of not conflicting, this area
Technical staff can be carried out the different embodiments or example and the feature of different embodiments or example described in this specification
With reference to and combination.
Although embodiment of the present utility model has been shown and described above, it is to be understood that above-described embodiment is
Exemplary, it is impossible to it is interpreted as to limitation of the present utility model, one of ordinary skill in the art is in the scope of the utility model
It is interior above-described embodiment to be changed, changed, replaced and modification.
Claims (5)
1. the on-line measuring device of medium pH value in a kind of bioreactor, including：Bioreactor joint
(1), input duct (2), detection cell (3) and overflow pipe (5)；Wherein, one end of the input duct (2) and the detection cell
(3) penetrate, the other end is connected with bioreactor joint (1)；The overflow pipe (5) connects with detection cell (3).
2. according to the on-line measuring device of medium pH value in the bioreactor described in claim 1, it is characterised in that
The overflow pipe (5) and the bottom of detection cell (3) connect.
3. according to the on-line measuring device of medium pH value in the bioreactor described in claim 1, it is characterised in that
PH value detection means (4) is set in detection cell (3).
4. according to the on-line measuring device of medium pH value in the bioreactor described in claim 3, it is characterised in that
The pH value detection means (4) is pH detectors, pH electrodes, pH detection probes or pH meter.
5. according to the on-line measuring device of medium pH value in the bioreactor described in claim 3 or 4, its feature exists
In the pH value detection means (4) is pH electrodes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721006177.8U CN207175972U (en) | 2017-08-12 | 2017-08-12 | The pH on-line measuring devices of nutrient solution in bioreactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721006177.8U CN207175972U (en) | 2017-08-12 | 2017-08-12 | The pH on-line measuring devices of nutrient solution in bioreactor |
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| CN207175972U true CN207175972U (en) | 2018-04-03 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110196220A (en) * | 2019-05-10 | 2019-09-03 | 无锡瑞生医疗科技有限公司 | High-throughput unicellular on-line detecting system |
-
2017
- 2017-08-12 CN CN201721006177.8U patent/CN207175972U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110196220A (en) * | 2019-05-10 | 2019-09-03 | 无锡瑞生医疗科技有限公司 | High-throughput unicellular on-line detecting system |
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| CP03 | Change of name, title or address |
Address after: 252201 No. 78 Ajiao Street, Donga County, Liaocheng City, Shandong Province Patentee after: Huarun Onde Biopharmaceutical Co., Ltd. Address before: 252201 No. 78 Ajiao Street, Donga County, Liaocheng City, Shandong Province Patentee before: Angde Biopharmaceutical Co., Ltd. |