CN207011386U - White backed planthopper passes the simulated environment closed system of poison to southern rice black-streaked dwarf disease - Google Patents
White backed planthopper passes the simulated environment closed system of poison to southern rice black-streaked dwarf disease Download PDFInfo
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- CN207011386U CN207011386U CN201720392988.XU CN201720392988U CN207011386U CN 207011386 U CN207011386 U CN 207011386U CN 201720392988 U CN201720392988 U CN 201720392988U CN 207011386 U CN207011386 U CN 207011386U
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- closed system
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The utility model discloses a kind of simulated environment closed system for passing poison to southern rice black-streaked dwarf disease for white backed planthopper, belongs to biological technical field.It is of the present utility model that southern rice black-streaked dwarf disease is passed in the simulated environment closed system of poison at least provided with 4 independent functional compartments for white backed planthopper, wherein 4 functional compartments are used separately as Rice Cropping room, insectary, raising malicious room, connect malicious room；Relative humidity in each functional compartments of the simulated environment closed system is 70%, and temperature control is daily to ensure illumination half a day at 24 25.5 DEG C.Carry out that white backed planthopper is high to the Virus spreading rate of southern rice black-streaked dwarf disease, stability is good using the simulated environment closed system provided by the utility model for passing poison to southern rice black-streaked dwarf disease for white backed planthopper.
Description
Technical field
It the utility model is related to biological technical field, and in particular to one kind is in simulated environment using white backed planthopper to south
The biography poison system of square black streaked dwarf virus of rice.
Background technology
Southern rice black-streaked dwarf virus (Southern rice black-streaked dwarf virus,
SRBSDV) be Reoviridae Reoviridae Fijiviruses category Fijivirus a novel species, with rice black-streaked dwarf
Viral (Rice black-streaked dwarf virus, RBSDV) belongs to together not of the same race.Caused by rice black-streaked dwarf virus
Black streaked dwarf virus of rice mainly propagated by small brown rice planthopper with persistent fashion, natural host includes the standing grain such as corn, rice and wheat
Undergraduate course crop.And the southern rice black-streaked dwarf disease as caused by southern rice black-streaked dwarf virus is mainly by white backed planthopper band poison
Propagate, be the main pathogen for causing the provinces and regions such as North Vietnam and south China rice dwarf virus disease in recent years.White backed planthopper is at me
It can not be survived the winter with northern territory in the middle part of state, so the disease is all confined to south China provinces and regions.In recent years, south rice secret note is short
Contracting disease saves population outbreak, according to incompletely statistics, injured area in 2009 in China Jiangxi, Hunan, Guangdong, Hubei and Hainan etc.
More than 300,000 hm2, about 6500hm2 rice, which loses, to be received.
At present, the prevention and control of southern rice black-streaked dwarf disease mainly use the measure of " controlling worm diseases prevention ", are typically to use early stage
Insecticide kills planthopper and passes poison ring section to block.But the uncertainty that white backed planthopper migrates, caused to " controlling worm diseases prevention "
Certain difficulty.Therefore, the other effective agents explored beyond insecticide are significant.In the preventing and treating of the viroses of plant
In, the use of antivirotic is one of alternative approach.Antiviral derivant is that a series of disease-resistant defence of energy activated plant are anti-
The material answered, it is in widespread attention in terms of the preventing and treating of plant virus.Because southern rice black-streaked dwarf disease is by carrying on the back in vain
Plant hopper is propagated with poison, the natural occurrence situation of each cell be difficult it is consistent, same processing different districts it is repeated poor,
Test data is difficult to analyze.Therefore, the utility model is sick to southern rice black-streaked dwarf using white backed planthopper in simulated environment
Biography poison it is particularly important in Chemicals.
Meanwhile disease-resistant variety screening is to prevent and treat another important channel of southern rice black-streaked dwarf disease.White backed planthopper migrates
Uncertainty, different field worm amount sizes are uneven, different year worm amount differ greatly and move into Time Inconsistency etc. because
Element, the screening zone to field disease-resistant variety carry out certain difficulty.By utilizing white backed planthopper black to south rice in simulated environment
The biography poison of bar dwarf wilt screens significant for disease-resistant variety.But it is high, steady not have Virus spreading rate in the prior art
The qualitative good device for being used for white backed planthopper and poison being passed to southern rice black-streaked dwarf disease.
Utility model content
Therefore, the technical problems to be solved in the utility model is to overcome that to lack Virus spreading rate in the prior art high, stably
Property it is good with white backed planthopper to the biography poison device of southern rice black-streaked dwarf disease the defects of, a kind of be used for white backward flight so as to provide
Lice passes the simulated environment closed system of poison to southern rice black-streaked dwarf disease.
Therefore, the technical solution of the utility model is as follows：
White backed planthopper passes the simulated environment closed system of poison to southern rice black-streaked dwarf disease, and the simulated environment is closed
At least provided with 4 independent functional compartments in system, wherein 4 functional compartments are used separately as Rice Cropping room, insectary, raising poison
Room, connect malicious room；Relative humidity in each functional compartments of the simulated environment closed system is 70%, and temperature control is in 24-25.5
DEG C, it is daily to ensure illumination half a day.
The simulated environment closed system is divided at least four separate functional compartments using clear glass, it is described
The outside of simulated environment closed system is provided with sliding door；The simulated environment closed system is one or more layers.
Fluorescent lamp is equipped with each functional compartments to be used to ensure the illumination in each functional compartments.
The simulated environment closed system also includes the box type container of 10 centimetres of 50 cm x, 30 cm x.
60 centimetres of 60 centimetres of a height of 200 cm x, 60 cm x of length and width or 100 cm x, 60 cm x of the zooecium.
Per 0.15m on every layer of layer top of the simulated environment closed system21 lamp is installed, the length of every lamp is
50cm。
The insectary, described to raise malicious room, described to connect malicious room with referred to as zooecium, the simulated environment closed system is set up and down
Two layers, 2 zooeciums of lower floor, 1, upper strata zooecium.
All lamps form lighting system by a switch control, and the switching system is connected by a plug wire with plug
Connect.
It is also equipped with fly net, humidifier, hygrometer, thermometer；
The fly net is made up of the nylon yarn of 30-40 mesh；
The sliding door is aluminium alloy sliding door.
The bottom of the simulated environment closed system is provided with universal wheel.
Technical solutions of the utility model, have the following advantages that：
1st, it is provided by the utility model closed to the simulated environment of southern rice black-streaked dwarf disease biography poison for white backed planthopper
System is used for white backed planthopper to be had to southern rice black-streaked dwarf disease biography poison compared with field by the uncertain white backed planthopper biography poison that migrates
The advantages of stability is good, Virus spreading rate is high.
2nd, it is provided by the utility model closed to the simulated environment of southern rice black-streaked dwarf disease biography poison for white backed planthopper
System can make to obtain poison for trying different 3 leaf of rice varieties, 1 heart stage seedling, then be transplanted in crop field, compare by manually passing poison
Different cultivars is to the southern rice black-streaked dwarf disease-resistant degree of disease so as to filtering out the rice product of anti-southern rice black-streaked dwarf virus
Kind.
3rd, it is provided by the utility model closed to the simulated environment of southern rice black-streaked dwarf disease biography poison for white backed planthopper
System can the larger rice seedlings of planting number, be adapted to high-volume to plant, the disease resistance of individual kind more than 50 can be identified simultaneously, simultaneously
The prevention effect of Chinese medicine more than 50 is determined, and simulated environment closed system of the present utility model can uniformly place multiple 50 lis
The box type container of rice × 10 centimetres of 30 cm x, condition of culture is unified, and qualification result is more accurate.
4th, it is provided by the utility model close in the simulated environment for passing poison to southern rice black-streaked dwarf disease for white backed planthopper
The temperature closed in system is 25 DEG C, daily half a day (12h) illumination access expansion environment, and effect is good.
5th, it is provided by the utility model closed to the simulated environment of southern rice black-streaked dwarf disease biography poison for white backed planthopper
The rice seedlings after poison will be connect in system to be transplanted in the paddy field for being covered with fly net, normal management is pressed in rice field, and RT-PCR is used after 20 days
Method detection band poison rate, reaches more than 85% with malicious rate.
Brief description of the drawings
, below will be right in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art, it should be apparent that, describe below
In accompanying drawing be some embodiments of the present utility model, for those of ordinary skill in the art, do not paying creativeness
On the premise of work, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the simulated environment closed system structural representation that the utility model uses；
Fig. 2 is the preferable box type container of the utility model；
Reference：
1- is switched；2- universal wheels；3- humidifiers；4- sliding doors.
Embodiment
It is to preferably further understand the utility model to provide following embodiments, it is not limited to the optimal implementation
Mode, content of the present utility model and protection domain are not construed as limiting, anyone is under enlightenment of the present utility model or incites somebody to action
As any and the utility model that the feature of the utility model and other prior arts is combined and drawn is same or like
Product, all fall within the scope of protection of the utility model.
Unreceipted specific experiment step or condition person in embodiment, according to the normal experiment described by document in the art
The operation of step or condition can be carried out.Agents useful for same or the unreceipted production firm person of instrument, being can be by acquisition purchased in market
Conventional reagent product.
Embodiment 1.
The present embodiment provides a kind of closed system of simulated environment for passing poison to southern rice black-streaked dwarf disease for white backed planthopper
System, as shown in figure 1,
A kind of simulated environment closed system for passing poison to southern rice black-streaked dwarf disease for white backed planthopper, the analog loop
At least provided with 4 independent functional compartments in the closed system of border, wherein 4 functional compartments are used separately as Rice Cropping room, insectary,
Malicious room is raised, connects malicious room；Relative humidity in each functional compartments of the simulated environment closed system is 70%, and temperature control is in 24-
It is 25.5 DEG C, daily to ensure illumination half a day.
The simulated environment closed system is divided at least four separate functional compartments using clear glass, it is described
The outside of simulated environment closed system is provided with sliding door 4；The simulated environment closed system is one or more layers.
Fluorescent lamp is equipped with each functional compartments to be used to ensure the illumination in each functional compartments.
The simulated environment closed system also includes the box type container of 10 centimetres of 50 cm x, 30 cm x.
60 centimetres of 60 centimetres of a height of 200 cm x, 60 cm x of length and width or 100 cm x, 60 cm x of the zooecium.
Per 0.15m on every layer of layer top of the simulated environment closed system21 lamp is installed, the length of every lamp is
50cm。
The insectary, described to raise malicious room, described to connect malicious room with referred to as zooecium, the simulated environment closed system is set up and down
Two layers, 2 zooeciums of lower floor, 1, upper strata zooecium.
All lamps form lighting system by the control of switch 1, and the switching system is connected by a plug wire with plug
Connect.
It is also equipped with fly net, humidifier 3, hygrometer, thermometer；
The fly net is made up of the nylon yarn of 30-40 mesh；
The sliding door 4 is aluminium alloy sliding door.
The bottom of the simulated environment closed system is provided with universal wheel 2.
The closed system of simulated environment for being used for white backed planthopper and poison being passed to southern rice black-streaked dwarf disease of the embodiment 1 of embodiment 2.
The application of system
(1) plantation of rice
Rice seed soaking vernalization, then it is seeded in the box type container of 10 centimetres of 50 cm x, 30 cm x and is placed in analog loop
In the Rice Cropping room of border closed system.After 8~10 days, rice seedlings are transplanted in the paddy field for being covered with fly net, until 3 leaves 1
Heart stage, raising and biography poison for white backed planthopper.
(2) raising of white backed planthopper
White backed planthopper is cultivated in the insectary of simulated environment closed system, treating plantation to the rice seedlings in box type container
Growing to the heart stage of 3 leaf 1 is gone in the insectary of simulated environment closed system, and room temperature is adjusted to 25 DEG C, and opening is turned off the light day, often
Day half a day (12h) illumination.Rice seedlings are regularly replaced, to keep the continuation of rice seedlings.
(3) acquisition with malicious seedling
The doubtful diseased plant of field collection southern rice black-streaked dwarf disease, takes back laboratory, is detected with RT-PCR method, really
Fixed its carries southern rice black-streaked dwarf virus, is planted in the feeding poison interior for the simulated environment closed system for being covered with fly net
It is standby, or part diseased plant is stored in -80 DEG C of refrigerators simultaneously.
(4) poison (or raising poison) is obtained
The white backed planthopper 3 age nymph raised in the insectary of simulated environment closed system raises poison 12~22 hours.
(5) poison is passed
3 age white backed planthopper nymphs raise poison 12~22 hours, are transferred to healthy seedling, continue raising 8~10 days, by 2~3 heads/plant
Connect worm amount be connected to the heart stage of 3 leaf 1 for examination rice seedling on, connect poison 12~23 hours, caught up with worm once every 6 hours daily, make uniformly
Obtain poison.Rice seedling is transplanted in the paddy field for being covered with fly net, every kind of 3 cells of medicament, per cell 100 clumps, normal pipe is pressed in rice field
Reason.RT-PCR method detection band poison rates are used after 20 days, reach 85% with malicious rate.
(6) RT-PCR method detection band poison rate
Rice black-streaked dwarf virus (RBSDV) detection primer S9F/R (S9F:5’-
GGAATTCATGGCAGACCAAGAGCGGGGAG-3’；
S9R:5 '-CGCGGATCCTCAAACGTCCAATTTCAAGG-3 '), southern rice black-streaked dwarf virus
(SRBSDV) detection primer S10-oF/R (S10-oF:5’-CGCGTCATCTCAAACTACAG-3’； S10-oR:5’-
TTTGTCAGCATCTAAAGCGC-3’).Two pairs of primers press 1:The ratio mixing of 1 (final concentration is 0.2 μm of ol/L), annealing temperature
Spend for 53 DEG C.Total RNA are extracted and RT-PCR detections are carried out according to kit specification standard method.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among the protection domain that variation is created still in the utility model.
Claims (9)
1. white backed planthopper passes the simulated environment closed system of poison to southern rice black-streaked dwarf disease, it is characterised in that the simulation
At least provided with 4 independent functional compartments in environment closed system, wherein 4 functional compartments are used separately as Rice Cropping room, worm is supported
Room, malicious room is raised, connect malicious room；Relative humidity in each functional compartments of the simulated environment closed system is 70%, and temperature control exists
It is 24-25.5 DEG C, daily to ensure illumination half a day；
The simulated environment closed system is divided at least four separate functional compartments, the simulation using clear glass
The outside of environment closed system is provided with sliding door；The simulated environment closed system is one or more layers.
2. simulated environment closed system according to claim 1, it is characterised in that be equipped with fluorescent lamp in each functional compartments
For ensureing the illumination in each functional compartments.
3. simulated environment closed system according to claim 2, it is characterised in that the simulated environment closed system also wraps
Include the box type container of 10 centimetres of 50 cm x, 30 cm x.
4. simulated environment closed system according to claim 3, it is characterised in that a height of 200 lis of the length and width of the zooecium
60 centimetres of rice × 60 centimetres of 60 cm x or 100 cm x, 60 cm x.
5. simulated environment closed system according to claim 4, it is characterised in that the simulated environment closed system it is every
Per 0.15m on the layer top of layer21 lamp is installed, the length of every lamp is 50cm.
6. simulated environment closed system according to claim 5, it is characterised in that the insectary, it is described to raise malicious room, institute
State and connect malicious room with referred to as zooecium, the simulated environment closed system is set two layers up and down, 2 zooeciums of lower floor, 1, upper strata zooecium.
7. simulated environment closed system according to claim 6, it is characterised in that all lamps are by a switch control group
Into lighting system, the switching system is connected by a plug wire with plug.
8. simulated environment closed system according to claim 7, it is characterised in that be also equipped with fly net, humidifier, humidity
Meter, thermometer；
The fly net is made up of the nylon yarn of 30-40 mesh；
The sliding door is aluminium alloy sliding door.
9. simulated environment closed system according to claim 8, it is characterised in that the bottom of the simulated environment closed system
Portion is provided with universal wheel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720392988.XU CN207011386U (en) | 2017-04-14 | 2017-04-14 | White backed planthopper passes the simulated environment closed system of poison to southern rice black-streaked dwarf disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720392988.XU CN207011386U (en) | 2017-04-14 | 2017-04-14 | White backed planthopper passes the simulated environment closed system of poison to southern rice black-streaked dwarf disease |
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| CN207011386U true CN207011386U (en) | 2018-02-16 |
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| CN201720392988.XU Expired - Fee Related CN207011386U (en) | 2017-04-14 | 2017-04-14 | White backed planthopper passes the simulated environment closed system of poison to southern rice black-streaked dwarf disease |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113693033A (en) * | 2021-09-14 | 2021-11-26 | 广西壮族自治区农业科学院 | Method for evaluating resistance of rice variety to southern rice black-streaked dwarf disease based on artificial inoculation |
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2017
- 2017-04-14 CN CN201720392988.XU patent/CN207011386U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113693033A (en) * | 2021-09-14 | 2021-11-26 | 广西壮族自治区农业科学院 | Method for evaluating resistance of rice variety to southern rice black-streaked dwarf disease based on artificial inoculation |
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Granted publication date: 20180216 |