CN106995860A - It is a kind of that biography poison method and its application of the white backed planthopper to southern rice black-streaked dwarf disease are utilized in simulated environment - Google Patents
It is a kind of that biography poison method and its application of the white backed planthopper to southern rice black-streaked dwarf disease are utilized in simulated environment Download PDFInfo
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Abstract
The present invention discloses a kind of biography poison method and its application for utilizing white backed planthopper sick to southern rice black-streaked dwarf in simulated environment, belongs to biological technical field.The a kind of of the present invention utilizes white backed planthopper to the malicious method of biography of southern rice black-streaked dwarf disease in simulated environment, and using simulated environment closed system, the relative humidity in the simulated environment closed system is 70%, and temperature control is at 24 25.5 DEG C, daily illumination half a day.It is good using the Virus spreading rate height of the white backed planthopper biography malicious method sick to southern rice black-streaked dwarf, stability in simulated environment that the present invention is provided.
Description
Technical field
The present invention relates to biological technical field, and in particular to one kind is in simulated environment using white backed planthopper to south rice
The malicious method of the biography of black streak dwarf.
Background technology
Southern rice black-streaked dwarf virus (Southern rice black-streaked dwarf virus, SRBSDV)
It is the novel species that Reoviridae Reoviridae Fijiviruses belong to Fijivirus, with rice black-streaked dwarf virus (Rice
Black-streaked dwarf virus, RBSDV) belong to together it is not of the same race.The paddy rice secret note as caused by rice black-streaked dwarf virus
Dwarf wilt is mainly propagated by small brown rice planthopper with persistent fashion, and natural host includes the gramineous crops such as corn, paddy rice and wheat.And
The southern rice black-streaked dwarf disease as caused by southern rice black-streaked dwarf virus is main to be propagated by white backed planthopper band poison, is in recent years
Cause the main pathogen of the provinces and regions such as North Vietnam and south China rice dwarf virus disease.White backed planthopper is in Central China and northern territory
It can not survive the winter, so the disease is all confined to south China provinces and regions.In recent years, southern rice black-streaked dwarf disease is in China Jiangxi, lake
South, Guangdong, Hubei and Hainan etc. save population outbreak, according to incompletely statistics, and injured area in 2009 is more than 300,000 hm2, about
6500hm2 paddy rice, which is lost, receives.
At present, the prevention and control of southern rice black-streaked dwarf disease mainly use the measure of " controlling worm diseases prevention ", are typically to use early stage
Insecticide kills planthopper and passes poison ring section to block.But, the uncertainty that white backed planthopper migrates is caused to " controlling worm diseases prevention "
Certain difficulty.Therefore, the other effective agents explored beyond insecticide are significant.In the preventing and treating of the viroses of plant,
The use of antivirotic is one of alternative approach.Antiviral derivant is can a series of defense responses of activated plant
Material, it is in widespread attention in terms of the preventing and treating of plant virus.Because southern rice black-streaked dwarf disease is by white backed planthopper band
What poison was propagated, the natural occurrence situation of each cell is difficult consistent, repeated poor, the test data of same processing different districts
It is difficult to analyze.Therefore, the present invention is malicious to the biography of southern rice black-streaked dwarf disease in medicament using white backed planthopper in simulated environment
It is particularly important in screening.
Meanwhile, disease-resistant variety screening is another important channel for preventing and treating southern rice black-streaked dwarf disease.White backed planthopper migrates
Uncertainty, different field worm amount size is uneven, different year worm amount differs greatly and moved into the factors such as Time Inconsistency,
Screening zone to field disease-resistant variety carrys out certain difficulty.By short to south rice secret note using white backed planthopper in simulated environment
The biography poison for the disease that contracts screens significant for disease-resistant variety.But do not have Virus spreading rate height, stability in the prior art
Using white backed planthopper to the malicious method of the biography of southern rice black-streaked dwarf disease in good simulated environment.
The content of the invention
Therefore, the technical problem to be solved in the present invention is to overcome lacks that Virus spreading rate is high, stability is good in the prior art
Simulated environment in using defect of the white backed planthopper to the malicious method of biography of southern rice black-streaked dwarf disease, so as to provide one kind in mould
Near-ring is within the border using white backed planthopper to the malicious method and its application of the biography of southern rice black-streaked dwarf disease.
Therefore, technical scheme is as follows：
It is a kind of that white backed planthopper is utilized in simulated environment to the malicious method of the sick biography of southern rice black-streaked dwarf, using analog loop
Relative humidity in border closed system, the simulated environment closed system is 70%, and temperature control is at 24-25.5 DEG C, daily half
Its illumination.
At least provided with 4 independent functional compartments in the simulated environment closed system, wherein 4 functional compartments are used separately as
Rice Cropping room, insectary raises malicious room, connects malicious room.
Comprise the following steps：
(1) for the plantation of examination rice seedlings：Rice seed soaking vernalization, is then seeded in the box of 10 centimetres of 50 cm x, 30 cm x
In formula container, it is placed in the Rice Cropping room；
(2) raising of white backed planthopper：White backed planthopper is cultivated in the insectary, treating the water in the Rice Cropping room
Rice seedling grows to the heart stage of 3 leaf 1 and is gone in the insectary and raise worm；
(3) acquisition with malicious seedling：Field is gathered and the confirmed paddy rice diseased plant kind with southern rice black-streaked dwarf virus
Plant in solarium, or part diseased plant is stored in -80 DEG C of refrigerators；
(4) the feeding poison of white backed planthopper：The white backed planthopper 3 age nymph that step (3) is raised raises poison 12~22 hours；
(5) poison is passed：The 3 age white backed planthopper nymphs that step (4) is raised after poison connect worm to healthy seedling, continue to raise 8~10 days,
It is set to spend the phase of walking around to；Then white backed planthopper is transferred to on examination rice seedling, worm was caught up with once every 6 hours.
Step (5) connects worm and is connected to by 2~3 heads/plant of the worm amount that connects on the confession examination rice seedling of the heart stage of 3 leaf 1, connects poison 12~23 small
When.
The malicious method of described biography is in the application in Chemicals.
The medicament refers to antivirotic or antiviral derivant.
Application of the malicious method of described biography in being screened in resistant rice cultivars.
Described application, is carried out as follows：
(1) box type container in 10 centimetres of 50 cm x, 30 cm x will be sowed after the rice seed soaking vernalization of different cultivars
Interior, each kind broadcasts 3 boxes, per 120~150 seeds of box, if 3 repetitions.
(2) 3 leaf, the 1 heart stage rice seedlings of the different cultivars of step (1) are gone in insectary, with the feeding poison white back of the body of 12 hours
Plant hopper carries out connecing poison to each treated 3 leaf, 1 heart stage rice seedling；
After (3) 8~10 days, different cultivars rice seedlings are transplanted in the paddy field for being covered with fly net, every kind of medicament is 3 small
Area, per cell 120 clumps.
Application of the malicious method of described biography in screening southern rice black-streaked dwarf disease degree monoclonal antibody.
Technical solution of the present invention, has the following advantages that：
What the 1st, the present invention was provided utilizes biography poison method of the white backed planthopper to southern rice black-streaked dwarf disease in simulated environment
Passing poison by the uncertain white backed planthopper of migrating compared with field has the advantages that stability is good, Virus spreading rate is high.
What the 2nd, the present invention was provided utilizes biography poison method of the white backed planthopper to southern rice black-streaked dwarf disease in simulated environment
By manually passing poison, it can make to obtain poison for trying different 3 leaf of rice varieties, 1 heart stage seedling, then be transplanted in crop field, it is relatively more different
Kind is to the disease-resistant degree of southern rice black-streaked dwarf disease so as to filter out the rice varieties of anti-southern rice black-streaked dwarf virus.
What the 3rd, the present invention was provided utilizes biography poison method of the white backed planthopper to southern rice black-streaked dwarf disease in simulated environment
Using simulated environment closed system can the larger rice seedlings of planting number, be adapted to high-volume plant, more than 50 product can be identified simultaneously
The disease resistance planted, while the prevention effect of Chinese medicine more than 50 is determined, and the simulated environment closed system of the present invention can be unified
The box type container of multiple 10 centimetres of 50 cm x, 30 cm x is placed, condition of culture is unified, and qualification result is more accurate.
What the 4th, the present invention was provided utilizes biography poison method of the white backed planthopper to southern rice black-streaked dwarf disease in simulated environment
It is 25 DEG C to control the temperature in simulated environment closed system, daily half a day (12h) illumination access expansion environment, and effect is good.
What the 5th, the present invention was provided utilizes biography poison method of the white backed planthopper to southern rice black-streaked dwarf disease in simulated environment
The malicious rate that obtains raised under 12~22 hours feeding poison time of poison reaches higher level, continues to increase and raises the poison time, obtains malicious rate raising and does not show
Write, and seriously stunt with malicious seedling, growing way is weaker, it is easy to eaten extremely by white backed planthopper, raise malicious overlong time and easily cause white backward flight
Lice can be eaten so as to situation hungry to death without rice seedling.
What the 6th, the present invention was provided utilizes biography poison method 3 of the white backed planthopper to southern rice black-streaked dwarf disease in simulated environment
Age white backed planthopper nymph raises poison and is transferred within 12~23 hours healthy seedling, continues to raise 8~10 days, it is spent the phase of walking around to.By 2~3
Head/plant connect worm amount be connected to the heart stage of 3 leaf 1 for examination rice seedling on, connect poison 12~23 hours, caught up with worm once every 6 hours daily, make
Uniformly obtain poison.Rice seedling is transplanted in the paddy field for being covered with fly net, every kind of 3 cells of medicament, per cell 100 clumps, rice field is by just
Often management.With the malicious rate of RT-PCR method detection band after 20 days, the malicious rate of band reaches more than 85%.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art
The accompanying drawing used required in embodiment or description of the prior art is briefly described, it should be apparent that, in describing below
Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before creative work is not paid
Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the simulated environment closed system structural representation that the present invention is used；
Fig. 2 is currently preferred box type container；
Reference：
1- is switched；2- universal wheels；3- humidifiers；4- sliding doors.
Embodiment
Following embodiments is provided it is preferably to further understand the present invention, it is not limited to the optimal embodiment party
Formula, is not construed as limiting to present disclosure and protection domain, anyone the present invention enlightenment under or by the present invention and its
The feature of his prior art be combined and draw it is any with the present invention it is same or like as product, all fall within the present invention
Within protection domain.
Unreceipted specific experiment step or condition person in embodiment, according to the normal experiment described by document in the art
The operation of step or condition can be carried out.Agents useful for same or the unreceipted production firm person of instrument, are that can be obtained by purchased in market
Conventional reagent product.
Embodiment 1
(1) for the plantation of examination rice seedlings
Analog loop is placed in rice seed soaking vernalization, the box type container for being then seeded in 10 centimetres of 50 cm x, 30 cm x
In the Rice Cropping room of border closed system.After 8~10 days, rice seedlings are transplanted in the paddy field for being covered with fly net, until 3 leaves 1
Heart stage, raising and biography poison for white backed planthopper.
(2) raising of white backed planthopper
White backed planthopper is cultivated in the insectary of simulated environment closed system, treating plantation to the rice seedlings in box type container
Growing to the heart stage of 3 leaf 1 is gone in the insectary of simulated environment closed system, and by room temperature regulation to 25 DEG C, opening is turned off the light day, often
Day half a day (12h) illumination.Rice seedlings are regularly replaced, to keep the continuation of rice seedlings.
(3) acquisition with malicious seedling
The doubtful diseased plant of field collection southern rice black-streaked dwarf disease, takes back laboratory, is detected with RT-PCR method, really
Fixed its carries southern rice black-streaked dwarf virus, is planted in the feeding poison interior for the simulated environment closed system for being covered with fly net
It is standby, or part diseased plant is stored in -80 DEG C of refrigerators simultaneously.
(4) poison (or raising poison) is obtained
The white backed planthopper 3 age nymph raised in the insectary of simulated environment closed system raises poison 12~22 hours.
(5) poison is passed
3 age white backed planthopper nymphs raise poison 12~22 hours, are transferred to healthy seedling, continue to raise 8~10 days, spend it and walk around to
Phase.It is connected to by 2~3 heads/plant of the worm amount that connects on the confession examination rice seedling of the heart stage of 3 leaf 1, connects poison 12~23 hours, caught up with daily every 6 hours
Worm once, makes uniformly to obtain poison.Rice seedling is transplanted in the paddy field for being covered with fly net, every kind of 3 cells of medicament, per cell 100 clumps,
Press normal management in rice field.With the malicious rate of RT-PCR method detection band after 20 days, the malicious rate of band reaches 85%.
(6) the malicious rate of RT-PCR method detection band
Primer S9F/R (S9F of rice black-streaked dwarf virus (RBSDV) detection primer with reference to Zhang Songbai etc.:5’-
GGAATTCATGGCAGACCAAGAGCGGGGAG-3’；
S9R:5 '-CGCGGATCCTCAAACGTCCAATTTCAAGG-3 '), southern rice black-streaked dwarf virus (SRBSDV)
Detection primer refers to Wang Qiang primer S10-oF/R (S10-oF:5’-CGCGTCATCTCAAACTACAG-3’；
S10-oR:5’-TTTGTCAGCATCTAAAGCGC-3’).Two pairs of primers press 1:1 (final concentration is 0.2 μm of ol/L)
Ratio mixing, annealing temperature be 53 DEG C.Total RNA are extracted and RT-PCR detections are entered according to kit specification standard method
OK.
The screening of the antiviral derivant of embodiment 2.
2.1 antiviral derivant processing
Sowed after rice seed soaking vernalization in the box type container of 10 centimetres of 50 cm x, 30 cm x, each kind broadcasts 3 boxes,
Per 100~120 seeds of box, if 3 repetitions.3 leaf, the 1 heart stage rice seedlings of different disposal are sprayed into 50,100mg/kg respectively
5% amino-oligosaccharide (active ingredient, similarly hereinafter), 30, the 3% super quick albumen of 60mg/kg and the lentinans of 20,40mg/kg 1%,
Control sprays the sterilized water of same volume.Gone to after 24 hours in malicious 12~24 hours insectaries of feeding, worm one was caught up with every 6 hours
It is secondary, make uniformly to obtain poison,.After 8~10 days, rice seedling is transplanted in the paddy field for being covered with fly net, every kind of 3 cells of medicament, per small
Press normal management in 100 clumps, area, rice field.
2.2 disease survey and statistics
All cells are investigated 1 time after the neat fringe of paddy rice, and 100 clumps are investigated altogether, is investigated by 0~7 grade of grade scale.0 grade：
Plant is disease-free leaf；1 grade：Plant is without substantially stunting, within highly shorter than healthy tree 20%；3 grades：Plant stunts, highly shorter than healthy tree
21%~35%；5 grades：Plant seriously stunts, highly shorter than healthy tree by 36%~50%；7 grades：Plant stunts more than 50%.Respectively
Calculate the average disease index and preventive effect of each processing.Disease index and preventive effect formula are as follows：
Disease index=∑ (diseased plant numbers at different levels × typical value at different levels)/(investigation total strain number × superlative degree typical value) × 100
Preventive effect (%)=(blank district disease index-chemicals treatment district disease index)/blank district disease index
×100
2.3 data analysis
All experiments set 3 repetitions, carry out difference in 5% (P=0.05) level with DuncanShi duncan's new multiple range methods and show
The analysis of work property.
2.4 antiviral derivant prevention effects
The sick disease index significant difference of southern rice black-streaked dwarf of different antiviral derivant processing.Wherein, 100mg/
Preferably, disease index is respectively 54.86 and 52.86 to the effect of kg amino-oligosaccharides and the super quick albumen of 60mg/kg, far below control
Disease index 70.48, preventive effect is respectively 22.17% and 24.99%；The prevention effect of lentinan is not good enough, 20mg/kg and
Disease index under two concentration of 40mg/kg is respectively 65.53 and 62.86, and preventive effect is respectively 7.02% and 10.82% (table 1).
Prevention effect of the antiviral derivant to southern rice black-streaked dwarf disease under the greenhouse experiment of table 1
Data are the average value ± standard error of 3 repetitions in table.With after column data, different letters represent new through DuncanShi
Multiple range method examines notable in 5% level difference.Data are mean±SE of three replicates.Different
Letters are significant difference at 5%level by Duncan ' s new multiple range
test.
The screening of the antivirotic of embodiment 3.
The processing of 3.1 antivirotics
Sowed after rice seed soaking vernalization in 50 × 30 × 10cm box type container, each kind broadcasts 3 boxes, per box 100~
120 seeds, if 3 repetitions.Each processing 3 leaf, 1 heart stage rice seedling is carried out connecing poison, 8~10 with the poison white backed planthopper of 12 hours is raised
After it, seedling-raising box is removed into insectary, it is phonetic that 3 leaf, the 1 heart stage rice seedlings of different disposal are sprayed into 120,240mg/kg 6% respectively
Peptimycin, 300, the toxic fluoride phosphates of 600mg/kg 30%, 160, the Ningnanmycins of 320mg/kg 8%, and 1600,3200mg/kg
80% Moroxydine Hydrochloride, control sprays the sterilized water of same volume.Rice seedling is transplanted to the paddy field for being covered with fly net after spray 24h
Interior, every kind of medicament sets 3 cells, per cell 120 clumps, and normal management is pressed in rice field.
3.2 disease survey and statistics
All cells are investigated 1 time after the neat fringe of paddy rice, and 100 clumps are investigated altogether, is investigated by 0~7 grade of grade scale.0 grade：
Plant is disease-free leaf；1 grade：Plant is without substantially stunting, within highly shorter than healthy tree 20%；3 grades：Plant stunts, highly shorter than healthy tree
21%~35%；5 grades：Plant seriously stunts, highly shorter than healthy tree by 36%~50%；7 grades：Plant stunts more than 50%.Respectively
Calculate the average disease index and preventive effect of each processing.Disease index and preventive effect formula are as follows：
Disease index=∑ (diseased plant numbers at different levels × typical value at different levels)/(investigation total strain number × superlative degree typical value) × 100
Preventive effect (%)=(blank district disease index-chemicals treatment district disease index)/blank district disease index
×100
3.3 data analysis
All experiments set 3 repetitions, carry out difference in 5% (P=0.05) level with DuncanShi duncan's new multiple range methods and show
The analysis of work property.
3.4 antivirotic prevention effects
The sick disease index significant difference of southern rice black-streaked dwarf of different antivirotic processing, wherein, 240mg/kg is phonetic
Preferably, disease index is 50.95 to the effect of peptimycin, and preventive effect is 28.67%；Next to that 120mg/kg cytosintetidemycins and 600mg/
Kg toxic fluoride phosphates, disease index is respectively 55.71 and 59.05, and preventive effect is respectively 22.00% and 17.34%.The disease index of control
For 71.43, hence it is evident that higher than above-mentioned 3 processing.The prevention effect of Moroxydine Hydrochloride is not good enough, 1600mg/kg and 3200mg/kg two
Disease index under concentration is respectively 66.66 and 63.81, and preventive effect is only 6.67% and 10.67% (table 2) respectively.
Prevention effect of the antivirotic to southern rice black-streaked dwarf disease under the greenhouse experiment of table 2
Data are the average value ± standard error of 3 repetitions in table.With after column data, different letters represent new through DuncanShi
Multiple range method examines notable in 5% level difference.Data are mean±SE of three replicates.Different
Letters are significant difference at 5%level by Duncan ' s new multiple range
test.
The screening of the resistant rice cultivars of embodiment 4.
4.1 manually connect poison
It is 54 commercially available kinds for trying different rice varieties.
Sowed after the rice seed soaking vernalization of different cultivars in the box type container of 10 centimetres of 50 cm x, 30 cm x, each
Kind broadcasts 3 boxes, per 120~150 seeds of box, if 3 repetitions.3 leaf, the 1 heart stage rice seedlings of different cultivars are gone into insectary
It is interior, each processing 3 leaf, 1 heart stage rice seedling is carried out connecing poison with the poison white backed planthopper of 12 hours is raised.After 8~10 days, by different cultivars water
Rice seedling is transplanted in the paddy field for being covered with fly net, every kind of 3 cells of medicament, per cell 120 clumps, and normal management is pressed in rice field.
4.2 disease survey and statistics
Varietal resistance screening test disease survey is in September, the last ten-days period are carried out, and paddy rice is in full heading time or pustulation period.Investigation
The sick clump rate of each cell, each cell investigates 100 clumps, and 3 cells are investigated altogether.
4.3 data analysis
All experiments set 3 repetitions, carry out difference in 5% (P=0.05) level with DuncanShi duncan's new multiple range methods and show
The analysis of work property.
The 4.4 disease-resistant results of different rice varieties
Resistance between rice varieties has larger difference.Kind of the average disease clump rate below 30% has 5, is disease-resistant
Kind, be respectively Yue You 9113, two line system, in excellent continent 481, excellent No. 1 of middle Zhejiang and Wandao 153, average disease clump rate is respectively
24%th, 28%, 27%, 27%, 28.7%, 29.3%；Average disease clump rate has 44 kinds of the grades of Jin You 77 30%~70%,
For susceptible variety；Average disease clump rate has 5 more than 75%, is high sense kind, is the perfume of day Feng You 85, three excellent 786, five respectively
Excellent 308, Jin Jiasi seedlings and Ao Long excellent 282, average disease clump rate is respectively 80.7%, 75.7%, 80.3%, 86.3% and 82.7%
(table 3).
The generation of the different rice varieties southern rice black-streaked dwarf diseases of table 3.
Comparative example
The band poison obtained using method disclosed in Chinese patent literature CN102630640A embodiments part is white
Backward flight lice, then passes poison and is transferred to healthy rice seedlings, continue to raise 1~15 day as follows, by single seedling 5, connect worm amount
Be connected to the heart stage of 3 leaf 1 on examination rice seedling, connecing poison 24 hours, caught up with worm once every 6 hours daily, make uniformly to obtain poison, then by rice seedling
It is transplanted in the paddy field for being covered with fly net.Using the malicious rate of RT-PCR method and testing conditions detection band of be the same as Example 1 after 20 days,
The malicious rate of band is only 70%.
Obviously, above-described embodiment is only intended to clearly illustrate example, and the not restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (9)
1. a kind of utilize biography poison method of the white backed planthopper to southern rice black-streaked dwarf disease in simulated environment, it is characterised in that
Using simulated environment closed system, the relative humidity in the simulated environment closed system is 70%, and temperature control is in 24-25.5
DEG C, daily illumination half a day.
2. according to the method described in claim 1, it is characterised in that at least provided with 4 in the simulated environment closed system
Independent functional compartments, wherein 4 functional compartments are used separately as Rice Cropping room, insectary raises malicious room, connects malicious room.
3. method according to claim 2, it is characterised in that comprise the following steps：
(1) for the plantation of examination rice seedlings：Rice seed soaking vernalization, the boxlike for being then seeded in 10 centimetres of 50 cm x, 30 cm x holds
In device, it is placed in the Rice Cropping room；
(2) raising of white backed planthopper：White backed planthopper is cultivated in the insectary, treating the rice seedlings in the Rice Cropping room
Growing to the heart stage of 3 leaf 1 is gone in the insectary and raises worm；
(3) acquisition with malicious seedling：Field gather and the confirmed paddy rice diseased plant with southern rice black-streaked dwarf virus plant in
In solarium, or part diseased plant is stored in -80 DEG C of refrigerators；
(4) the feeding poison of white backed planthopper：The white backed planthopper 3 age nymph that step (3) is raised raises poison 12~22 hours；
(5) poison is passed：The 3 age white backed planthopper nymphs that step (4) is raised after poison connect worm to healthy seedling, continue to raise 8~10 days, make it
Spend the phase of walking around to；Then white backed planthopper is transferred to on examination rice seedling, worm was caught up with once every 6 hours.
4. method according to claim 3, it is characterised in that step (5) connects worm and is connected to 3 by 2~3 heads/plant of the worm amount that connects
Supplying on examination rice seedling for the heart stage of leaf 1, connects poison 12~23 hours.
5. any malicious methods of described biography of claim 1-4 are in the application in Chemicals.
6. application according to claim 5, it is characterised in that the medicament refers to antivirotic or antiviral derivant.
7. application of any malicious methods of described biography of claim 1-4 in being screened in resistant rice cultivars.
8. application according to claim 7, it is characterised in that carry out as follows：
(1) it will be sowed after the rice seed soaking vernalization of different cultivars in the box type container of 10 centimetres of 50 cm x, 30 cm x, often
Individual kind broadcasts 3 boxes, per 120~150 seeds of box, if 3 repetitions.
(2) 3 leaf, the 1 heart stage rice seedlings of the different cultivars of step (1) are gone in insectary, with the feeding poison white backed planthopper of 12 hours
Each treated 3 leaf, 1 heart stage rice seedling is carried out connecing poison；
After (3) 8~10 days, different cultivars rice seedlings are transplanted in the paddy field for being covered with fly net, every kind of 3 cells of medicament, often
Cell 120 clumps.
9. application of any malicious methods of described biography of claim 1-4 in screening southern rice black-streaked dwarf disease degree monoclonal antibody.
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| CN109456947A (en) * | 2018-11-29 | 2019-03-12 | 湖南农业大学 | A kind of method that efficient indoor winter saves propagation southern rice black-streaked dwarf virus |
| CN113693033A (en) * | 2021-09-14 | 2021-11-26 | 广西壮族自治区农业科学院 | Method for evaluating resistance of rice variety to southern rice black-streaked dwarf disease based on artificial inoculation |
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| CN113693033B (en) * | 2021-09-14 | 2022-11-08 | 广西壮族自治区农业科学院 | Method for evaluating resistance of rice variety to southern rice black-streaked dwarf disease based on artificial inoculation |
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