CN206965212U - Bubble blocking apparatus suitable for chromatographic column - Google Patents
Bubble blocking apparatus suitable for chromatographic column Download PDFInfo
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- CN206965212U CN206965212U CN201720844471.XU CN201720844471U CN206965212U CN 206965212 U CN206965212 U CN 206965212U CN 201720844471 U CN201720844471 U CN 201720844471U CN 206965212 U CN206965212 U CN 206965212U
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- bubble
- pond
- drain pipe
- intercepts
- chromatographic column
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Abstract
The utility model discloses the bubble blocking apparatus suitable for chromatographic column, the bubble that the constant flow pump port of export is arranged on including connection intercepts pond, the top movable that bubble intercepts pond is provided with closure, intercepted close to bubble and be provided with air bleeding valve in the side wall of pond top open part, bubble intercepts bottom of pond portion and is provided with and communicated with the first drain pipe, the liquid outlet of first drain pipe is connected with the entrance point of membrane filtration module, the port of export of the membrane filtration module is provided with and communicated with the second drain pipe, check valve is provided with and communicated with second drain pipe, the liquid outlet of second drain pipe extends to the top of filler in chromatography master.The utility model utilizes bubble point principle, and being back to bubble by the resistance of filter membrane in liquid storage bottle during eluent deficiency, when gas flows through membrane filtration module intercepts in pond, is most discharged afterwards through air bleeding valve, effectively avoids gas from entering in chromatographic column, prevent filler from drying up；Bubble intercepts and is provided with closure at the top of pond, prevents eluent from evaporating into air, it is ensured that experiment safety.
Description
Technical field
Column chromatography for separation technology is the utility model is related to, more particularly, to a kind of bubble blocking apparatus suitable for chromatographic column.
Background technology
Column chromatography is also known as chromatography or chromatography, and it is a kind of conventional method of purification, and cardinal principle is according to material
Adsorption capacity in stationary phase is different and is separated, and is usually used in the separation of the materials such as natural products, protein.Chromatographic column
Filler situation etc. is to influence the principal element of chromatography result in filling, the selection of eluent and chromatography process.Chromatographic column fills
More using addition eluent manually after the completion of filling out, some chromatography pillars are larger, and elution dosage is big, and labor intensity is big；For in solution
Technical problem is stated, it is existing that eluent is added into chromatographic column using constant flow pump more, but in chromatography process, experimenter needs
Moment concern liquid storage bottle in eluent, with prevent experimenter because cause thing is left gas through constant flow pump enter pillar in and
Form bubble or cause filler to dry up, in turn result in the failure of an experiment, be unfavorable for experimental period arrangement；In addition, carrying out molecular sieve
When, because sample size is less, and crossover sub and filler are combined closely, and can not effectively be kept away carrying out sample and elution fluid exchange
Exempt from the entrance of bubble, cause chromatographic stuffing to dry up, influence to test.
The content of the invention
The utility model purpose be to provide it is a kind of it is simple in construction, have and intercept bubble and be applied to column chromatography pillar
Special bubble blocking apparatus.
To achieve the above object, the utility model takes following technical proposals：
Bubble blocking apparatus described in the utility model suitable for chromatographic column, including connection are arranged on the gas of the constant flow pump port of export
Bubble intercepts pond, and the top movable that the bubble intercepts pond is provided with closure, and the side wall of pond top open part is intercepted close to bubble
On be provided with air bleeding valve, bubble intercepts bottom of pond portion and is provided with and communicated with the first drain pipe, the liquid outlet and film of first drain pipe
The entrance point of filter assemblies is connected, and the port of export of the membrane filtration module is provided with and communicated with the second drain pipe, the second drain pipe
On be provided with and communicated with check valve, the liquid outlet of second drain pipe extend to chromatography it is main in filler top.
The membrane filtration module is hyperfiltration membrane assembly.
The utility model advantage is simple in construction, effectively avoids the occurrence of filler dry-out.The utility model utilizes bubble
Point principle, in liquid storage bottle during eluent deficiency, is back to bubble by the resistance of filter membrane when gas flows through membrane filtration module and blocks
Cut in pond, most discharged afterwards through air bleeding valve, effectively avoid gas from entering in chromatographic column, prevent filler from drying up；Bubble is intercepted at the top of pond
Closure is provided with, prevents eluent from evaporating into air, it is ensured that experiment safety.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
As shown in figure 1, the bubble blocking apparatus described in the utility model suitable for chromatographic column, including connection are arranged on constant flow pump
The bubble of 1 port of export intercepts pond 2, and the top movable that the bubble intercepts pond 2 is provided with closure 3, prevents eluent from volatilizing, really
Protect experiment safety；Intercepted close to bubble and air bleeding valve 4 is provided with the side wall of the top open part of pond 2, bubble intercepts the connection of the bottom of pond 2
The first drain pipe 5 is provided with, the liquid outlet of first drain pipe 5 is connected with the entrance point of membrane filtration module 6, for obstructing
Air, the port of export of membrane filtration module 6 are provided with and communicated with the second drain pipe 7, check valve 8 are provided with and communicated with the second drain pipe 7,
The liquid outlet of second drain pipe 7 extends to the top of filler in chromatographic column 9.
During actual manufacture, for ease of observation, bubble intercepts pond 2 and is made up of transparent plastics material.
During actual assembled, membrane filtration module 6 is hyperfiltration membrane assembly, 0.45 μm of the diameter 50mm of milipore filter, aperture, bubble point pressure
0.269~0.294MPa of power；Constant flow pump 1 exports pressure and is using relatively low peristaltic pump, flow velocity 0.5-2ml/min, work is pumped
3kg/cm2。
In use, the entrance point of the pump line of constant flow pump 1 is inserted into liquid storage bottle 10, its port of export and bubble are intercepted into pond 2
It is connected, opens constant flow pump 1 and check valve 8, the air in eluent flow pipe is discharged, is then shut off constant flow pump 1 and list
To valve 8, the second drain pipe 7 is suspended in chromatographic column 9 above filler, opens constant flow pump 1, air bleeding valve 4 and check valve 8, regulation
The flow velocity of constant flow pump 1, the eluent in liquid storage bottle 10 successively intercept pond 2, the first drain pipe 5, membrane filtration through constant flow pump 1, bubble
The drain pipe 7 of component 6 and second enters in chromatographic column；When eluent deficiency in liquid storage bottle 10, enter gas in pump line, according to bubble
Point principle, it is back in bubble interception pond 2 by resistance when gas flows through membrane filtration module 6 and is discharged through air bleeding valve 4.
Claims (2)
- A kind of 1. bubble blocking apparatus suitable for chromatographic column, it is characterised in that：Constant flow pump is arranged on including connection（1）The port of export Bubble intercepts pond（2）, the bubble interception pond（2）Top movable be provided with closure（3）, pond is intercepted close to bubble（2）Top Air bleeding valve is provided with the side wall of portion's opening（4）, bubble interception pond（2）Bottom is provided with and communicated with the first drain pipe（5）, it is described First drain pipe（5）Liquid outlet and membrane filtration module（6）Entrance point be connected, the membrane filtration module（6）The port of export It is provided with and communicated with the second drain pipe（7）, the second drain pipe（7）On be provided with and communicated with check valve（8）, second drain pipe（7） Liquid outlet extend to chromatographic column（9）The top of interior filler.
- 2. it is suitable to the bubble blocking apparatus of chromatographic column according to claim 1, it is characterised in that：The membrane filtration module（6） For hyperfiltration membrane assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720844471.XU CN206965212U (en) | 2017-07-13 | 2017-07-13 | Bubble blocking apparatus suitable for chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720844471.XU CN206965212U (en) | 2017-07-13 | 2017-07-13 | Bubble blocking apparatus suitable for chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206965212U true CN206965212U (en) | 2018-02-06 |
Family
ID=61401740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720844471.XU Active CN206965212U (en) | 2017-07-13 | 2017-07-13 | Bubble blocking apparatus suitable for chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206965212U (en) |
-
2017
- 2017-07-13 CN CN201720844471.XU patent/CN206965212U/en active Active
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