CN208649284U - It is a kind of that amplification module is extracted based on micro-fluidic full-automatic DNA - Google Patents
It is a kind of that amplification module is extracted based on micro-fluidic full-automatic DNA Download PDFInfo
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- CN208649284U CN208649284U CN201820775734.0U CN201820775734U CN208649284U CN 208649284 U CN208649284 U CN 208649284U CN 201820775734 U CN201820775734 U CN 201820775734U CN 208649284 U CN208649284 U CN 208649284U
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Abstract
The utility model relates to gene analysis test technical fields, more particularly to a kind of extracted based on micro-fluidic full-automatic DNA expands module, amplification unit is extracted including at least one DNA, it includes: lysate storage pool, amplification system storage pool, amplification chamber, filter device that DNA, which extracts amplification unit, lysate storage pool, amplification system storage pool pass through pipeline respectively and are connected to the entrance for expanding chamber, and the inlet of the amplification chamber is arranged in filter device；The outlet of amplification chamber is connected to pipe outlet by pipeline, is communicated on the pipeline between amplification chamber and pipe outlet and closed liquid is taken to export；Lysate storage pool, amplification system storage pool, pipe outlet three and the on off operating mode of amplification chamber can switch respectively.Whole reagents are stored on chip by the utility model in liquid form, are realized detection by switching pipeline, are simplified operation, detection speed is fast, and consumption sample is few；Mode based on filter device filtering retention carries out DNA extraction operation, and chip greatly simplifies, and manufacturing cost reduces.
Description
Technical field
The utility model relates to gene analysis test technical fields more particularly to a kind of based on micro-fluidic full-automatic DNA
Extract amplification module.
Background technique
With the continuous development of human genome sequencing project successfully completed with next-generation DNA sequencing technology, gene point
Analysis detection is just increasingly obtaining the attention of people, and is gradually being applied to health care, environmental protection and judicial expertise etc. many
It is multi-field.For the development and application for further pushing genetic analysis, realizing the low cost of entire testing process, rapidly and automatically changing is
Very necessary.Conventional foranalysis of nucleic acids process generally comprises the extraction of nucleic acid, the enzymatic amplification reaction in region to be measured and product
Three steps such as separation detection.Time-consuming for whole process, step is more, needs to have special technology in specialized laboratory
Personnel carry out, but also need to use a large amount of large-scale special equipment.
Traditional method for extracting nucleic acid includes two kinds: first is that phenol chloroform method.Second is that the various shapes based on Solid Phase Extraction
The method for extracting nucleic acid of formula.For based on phenol chloroform method, the organic reagent for needing to use is more, wherein being no lack of one
A little poisonous and harmful substances, and for low-cost plastic chip, the storage on chip of organic reagent is often
Chip is dissolved, therefore this method is not appropriate for being transplanted on micro-fluidic chip and carrying out, Integrated automation is not known where to begin yet.Base
Nucleic acid molecules are mainly adsorbed on to the table of the solid matters such as silica, glass, diatomite in the nucleic acid extraction of Solid Phase Extraction
Extraction purification is realized in face.Many commercially available kits all take this method at present, and form also includes spe membrane, extraction
Take column, microballoon, powder and magnetic bead etc..Solid phase extraction is the technology of existing more mainstream on microfluidic platform, but is used
After this method carries out nucleic acid extraction, use high chaotropic saline solutions that can generate inhibiting effect to the amplified reaction of rear end, therefore
Need the purity for guaranteeing to extract product using complicated complicated elution action.This just directly improves the complexity of microfluidic system
Degree and processing cost, the microfluidic system that existing Solid Phase Extraction extracts nucleic acid is the silicon such as the high glass of use cost mostly
Sill makes, technique very complicated, and a large amount of manpower is needed to carry out, and manufacturing process is also needed using some toxicity and harm
The biggish chemicals of property, industrialization promotion are very difficult.
Utility model content
(1) technical problems to be solved
The purpose of this utility model be against the above deficiency, provide a kind of high speed, efficiently, consumption sample it is few and low in cost
Amplification module is extracted based on micro-fluidic full-automatic DNA.
(2) technical solution
In order to solve the above-mentioned technical problem, the utility model is provided a kind of extracted based on micro-fluidic full-automatic DNA and expanded
Increase module, including at least one DNA extracts amplification unit, it includes: lysate storage pool, amplification that the DNA, which extracts amplification unit,
System storage pool, amplification chamber, filter device, the lysate storage pool, amplification system storage pool pass through pipeline and institute respectively
The entrance connection of amplification chamber is stated, the inlet of the amplification chamber is arranged in the filter device；The amplification chamber goes out
Mouthful pipe outlet is connected to by pipeline, is communicated on the pipeline expanded between chamber and pipe outlet and closed takes liquid
Outlet；The lysate storage pool, amplification system storage pool, pipe outlet three and the on off operating mode of the amplification chamber can divide
Do not switch.
Wherein, the outlet of the lysate storage pool is connected with the first Micro-flow pipe, in first Micro-flow pipe
Equipped with the first integrated chip valve；The outlet of the amplification system storage pool is connected with the second Micro-flow pipe, second miniflow
Keyholed back plate road is equipped with the second integrated chip valve；First Micro-flow pipe and the second Micro-flow pipe convergence after with total pipeline
First end connection, the second end of the total pipeline are connected to the entrance of the amplification chamber；The outlet of the amplification chamber and institute
State and be connected between pipe outlet by third Micro-flow pipe, positioned at it is described amplification chamber outlet and it is described take liquid export between
The third Micro-flow pipe be equipped with third integrated chip valve.
Wherein, the connectivity part of the lysate storage pool and first Micro-flow pipe is equipped with strainer.
Wherein, the screen surface contact angle is greater than 90 degree.
Wherein, the chip liquid storing part for further including chip pipe main body and being attached thereto, the lysate storage pool and amplification
System storage pool is arranged in the chip liquid storing part, the amplification chamber, the first Micro-flow pipe, the second miniflow keyholed back plate
Road, third Micro-flow pipe are respectively positioned in the chip pipe main body, the first integrated chip valve, the second integrated chip valve,
Third integrated chip valve takes liquid outlet, pipe outlet to be arranged on the chip pipe main body.
Wherein, the chip pipe main body is composed of upper layer chip with lower layer chip.
Wherein, the total pipeline is the groove structure being arranged on the lower layer chip top surface, and the amplification chamber is to set
Set the groove structure in the upper layer die bottom surface；After the upper layer chip is connect with lower layer chip, the total pipeline is cut
Only place extends to the lower section of the amplification chamber to realize the connection with the amplification chamber；The filter device include filter paper and
The fence being arranged around the edge of the filter paper, the filter paper level are laid on the connection at the cut-off with the amplification chamber
Place.
Wherein, heating refrigeration component is equipped at the amplification chamber.
Wherein, the upper layer chip, which is located in the amplification chamber, is equipped with the more miniature cylinders extended downwardly, the mistake
Filter device is located at the indoor edge of the amplification chamber to be fixed by the miniature cylinder, and the remaining edge of the filter device is pressed in institute
It states between upper layer chip and lower layer chip.
Wherein, region and the filter paper are circle at the cut-off, and the center of circle of the filter paper is located at the cut-off
The downstream at place, the center of circle of the filter paper are located at the cut-off except region.
(3) beneficial effect
The above-mentioned technical proposal of the utility model has the advantages that the utility model by whole examinations compared with prior art
Agent is stored on chip in liquid form, is realized detection by switching pipeline, is simplified operation, detection speed is fast, and consumption sample is few；
Mode based on filter device filtering retention carries out DNA extraction operation, and chip greatly simplifies, and manufacturing cost reduces.
Detailed description of the invention
Fig. 1 is the axonometric drawing for extracting amplification module described in the utility model embodiment based on micro-fluidic full-automatic DNA；
Fig. 2 is the lateral sectional view of chip liquid storing part described in the utility model embodiment；
Fig. 3 is the lateral sectional view that chamber is expanded described in the utility model embodiment；
Fig. 4 is the top view that chamber is expanded described in the utility model embodiment.
In figure: 1, chip liquid storing part；2, lysate storage pool；3, amplification system storage pool；4, strainer；5, bottom opening；
6, the second integrated chip valve；7, the first integrated chip valve；8, filter device；9, chamber is expanded；10, third integrated chip valve；11,
Liquid is taken to export；12, pipe outlet；13, total pipeline；14, the first Micro-flow pipe；15, the second Micro-flow pipe；16, chip pipe
Road main body；17, linkage interface；18, heating refrigeration component；19, lower layer chip；20, upper layer chip；21, miniature cylinder；22,
At cut-off.
Specific embodiment
It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is a part of the embodiment of the utility model, instead of all the embodiments.Based on the reality in the utility model
Apply example, those of ordinary skill in the art's every other embodiment obtained without making creative work, all
Belong to the range of the utility model protection.
As shown in Figure 1, a kind of extracted based on micro-fluidic full-automatic DNA provided by the embodiment of the utility model expands mould
Block, including at least one DNA extract amplification unit, and it includes: lysate storage pool 2, amplification system that the DNA, which extracts amplification unit,
Storage pool 3, amplification chamber 9, filter device 8, the lysate storage pool 2, amplification system storage pool 3 pass through pipeline and institute respectively
The entrance connection of amplification chamber 9 is stated, the inlet of the amplification chamber 9 is arranged in the filter device 8；The amplification chamber 9
Outlet pipe outlet 12 is connected to by pipeline, being communicated on the pipeline between the amplification chamber 9 and pipe outlet 12 can
It is closed that liquid is taken to export 11；The lysate storage pool 2, amplification system storage pool 3,12 three of pipe outlet and the amplification chamber
The on off operating mode of room 9 can switch respectively.When specific operation, first blood, dried blood spot, buccal swab, saliva, stub equal samples are added
It is added in lysate storage pool 2, stands 2-5 minutes to guarantee that sample sufficiently cracks；After cracking sufficiently, set at pipe outlet 12
The liquid driving devices such as peristaltic pump or syringe pump are set, lysate storage pool 2 and amplification chamber 9, amplification chamber 9 and pipe is then connected
The connected state of road outlet 12, start liquid driving device make lysate and sample mixed liquor from lysate storage pool 2 flow out into
Enter to expand chamber 9, and then is flowed out again from pipe outlet 12；When mixing liquid flows through amplification chamber 9, it is dissolved in long-chain therein
DNA profiling will be retained by filter device 8.It is then shut off lysate storage pool 2 and expands the connection of chamber 9, open amplification body
It is the connection of storage pool 3 and amplification chamber 9, under the driving of peristaltic pump, the liquid in amplification system storage pool 3 flows into amplification chamber
Room 9；The primer as contained in amplification system is short chain DNA, will not be wound and retain by filter device 8, can uniformly dispersing
Among the entire space of amplification chamber 9, amplification chamber 9 and lysate storage pool 2, amplification chamber 9 and amplification are turned off at this time
System storage pool 3.The connection of 12 three of pipe outlet carries out PCR amplification expanding in chamber 9, PCR (polymerase chain reaction,
Also known as: polymerase chain reaction, English name: Polymerase Chain Reaction)；Filter device 8 is located at amplification chamber
In 9, amplification in situ is realized.After amplification, conducting amplification chamber 9 and lysate storage pool 2, amplification chamber 9 and amplification body
It is storage pool 3, amplified production is transferred to from amplification chamber 9 by the negative-pressure ward of liquid driving pump and is taken in liquid outlet 11.It takes
The hole that liquid outlet 11 is connected with amplification chamber 9, pipe outlet 12 respectively for a front and back end takes 11 top of liquid outlet to open wide；
Entire flow path can be made to close taking to shut above liquid outlet 11 using sealant tape, not influence liquid flowing；Product to be amplified
Be transferred to take liquid outlet 11 at when, sealant tape, which is raised, can be taken off amplified production.To guarantee lysate and amplification system
Lysate and amplification system liquid generally can be encapsulated in advance lysate storage pool 2 before factory and expanded by the validity of liquid
The opening that 2 top of lysate storage pool is opened in increasing system storage pool 3, when use, is added sample.The utility model will be complete
Portion's reagent is stored on chip in liquid form, simplifies operation, and detection speed is fast, and consumption sample is few；It is filtered based on filter device 8
The mode of retention carries out DNA extraction operation, and chip greatly simplifies, and manufacturing cost reduces.
Specifically, as shown in Figure 1, the outlet of the lysate storage pool 2 is connected with the first Micro-flow pipe 14, described
One Micro-flow pipe 14 is equipped with the first integrated chip valve 7；It is micro-fluidic that the outlet of the amplification system storage pool 3 is connected with second
Pipeline 15, second Micro-flow pipe 15 are equipped with the second integrated chip valve 6；First Micro-flow pipe 14 and second micro-
Flow control pipeline 15 is connected to after converging with the first end of total pipeline 13, the second end of the total pipeline 13 and the amplification chamber 9
Entrance connection；It is connected between the outlet and the pipe outlet 12 of the amplification chamber 9 by third Micro-flow pipe, is located at institute
The outlet for stating amplification chamber 9 and the third Micro-flow pipe taken between liquid outlet 11 are equipped with third integrated chip valve
10.First integrated chip valve 7 and the second integrated chip valve 6 be used to control the liquid in two pipelines enter expand chamber 9 when
Sequence.Existing PDMS chip mandril valve arrangement etc. can be used in integrated chip valve.PDMS (dimethyl silicone polymer, english abbreviation:
Polydimethylsiloxane), because its is at low cost, using simple, with having good adhesiveness between silicon wafer, and have
The features such as good chemical inertness, becomes a kind of polymer material for being widely used in micro-fluidic equal fields.Pass through control three
The state of integrated chip valve realizes the control to three pipelines in which can be convenient, easy to operate；The setting of total pipeline 13 is so that expand
Increasing chamber 9 need to be arranged an entrance and can while be connected to the first Micro-flow pipe 14, the second Micro-flow pipe 15, so that
Amplification system entrance structure simplifies, and facilitates the setting of filter device 8.
Further, as depicted in figs. 1 and 2, the connection of the lysate storage pool 2 and first Micro-flow pipe 14
Place is equipped with strainer 4.For the complex samples such as dried blood spot, stub, whole blood, the impurity in sample is often more, passes through strainer 4
Loose and porous structure the impurity of bulky grain can tentatively be filtered out so that enter amplification chamber 9 in DNA profiling have it is higher
Purity；In addition, can provide enough surface tension makes to crack when the range of aperture size of strainer 4 is 20 μm~100 μm
Liquid is able to maintain that its surface to prevent it from permeating into the first Micro-flow pipe 14.The peripheral circumferential of strainer 4 is fixed on cracking
2 subjacent of liquid storage pool causes strainer 4 with fluid motion to avoid due to fluid motion.
Further, 4 surface contact angle of strainer is greater than 90 degree.For the hydrophobicity for further promoting strainer 4, can filter
Net 4 or the fibrous material surface for making strainer 4 do certain hydrophobically modified, guarantee that final 4 surface contact angle of strainer is big
In 90 degree, phenomenon of osmosis occurs for lysate before avoiding detection from starting.
It, can be by the diameter for the bottom opening 5 being connected to the second Micro-flow pipe 15 for amplification system liquid storage tank
It is contracted to the state less than 2mm, due to air pressure in chip and the effect of liquid own face tension, in addition plastic chip pipeline
Surface is not water-wetted surface, and reagent can maintain interface at bottom opening 5, will not actively be permeated into pipeline.
In this way it is prevented that the reagent of storage enters Micro-flow pipe in the state of unused.
Further, as depicted in figs. 1 and 2, the application further includes chip pipe main body 16 and the chip being attached thereto storage
Liquid portion 1, the lysate storage pool 2 are arranged in the chip liquid storing part 1 with amplification system storage pool 3, the amplification chamber
Room 9, the first Micro-flow pipe 14, the second Micro-flow pipe 15, third Micro-flow pipe are respectively positioned on the chip pipe main body 16
Interior, the first integrated chip valve 7, third integrated chip valve 10, takes liquid to export 11, pipe outlet 12 at second integrated chip valve 6
It is arranged on the chip pipe main body 16.Chip liquid storing part 1 is fixed to each other with chip pipe main body 16, it is preferred to use same
Class is made with the material of the trade mark, convenient to carry out that connection is fully sealed at the linkage interface 17 of the two；It is described to there is pipeline to be respectively provided with
In chip pipe main body 16, it is not necessary that pipeline is separately provided, overall structure simplifies, and pipeline is hardly damaged；Chip liquid storing part 1 and core
Piece pipe main body 16 is individually processed, and liquid is reinjected after sealed connection, easy to process.
Further, as shown in figure 3, the chip pipe main body 16 is combined by upper layer chip 20 with lower layer chip 19 and
At.Pipeline can be formed by slotting in upper layer chip 20 and lower layer chip 19 on opposite in this way, without in chip pipeline master
Pipeline is re-layed in body 16, reduces manufacture difficulty, facilitates the design and arrangement of pipeline and chamber.Upper layer chip 20 and lower layer's core
The bonding pattern of piece 19 is preferably hot pressing or laser welding, to guarantee the leakproofness of the two junction.
Preferably, as shown in figure 3, the total pipeline 13 is the groove structure being arranged on 19 top surface of lower layer chip,
The amplification chamber 9 is the groove structure being arranged on 20 bottom surface of upper layer chip；The upper layer chip 20 and lower layer chip
After 19 connections, 22 extend to the lower section of the amplification chamber 9 to realize and the amplification chamber 9 at the cut-off of the total pipeline 13
Connection；The filter device 8 includes filter paper and the fence around the setting of the edge of the filter paper, and the filter paper level is laid on institute
State at cut-off the connectivity part of 22 with the amplification chamber 9.In this way when the lysate with sample reaches at amplification chamber 9, split
Cut-off 22 can be reached by the total pipeline 13 in lower layer chip 19 by solving liquid, and the amplification chamber of upper layer chip 20 is then entered through filter paper
In room 9；Long nucleic acid template in lysate can be wound by filter paper and retain, remaining substance can pass through filter paper continue flowing until from
The outflow of pipe outlet 12 becomes waste liquid.In lysate completely after the outflow of pipe outlet 12, the first integrated chip valve 7 is closed, the
Two integrated chip valves 6 are opened, and the liquid in amplification system liquid storage tank is pumped in amplification chamber 9 by sub-atmospheric pressure downstream；Due to amplification
Primer contained in system is short chain DNA, therefore will not be wound and retain by filter device 8, can be dispersed evenly to amplification chamber 9
Among entire space.At this point, the first integrated chip valve 7, the second integrated chip valve 6, third integrated chip valve 10 are closed again, i.e.,
It can carry out PCR amplification.
Preferably, heating refrigeration component 18 is equipped at the amplification chamber 9.It can be appreciated that in the chip pipeline
The upper surface of main body 16 and be located at the amplification chamber 9 at, be equipped with heating freeze component 18.It needs to adjust when PCR amplification to expand
Increase the temperature in chamber 9, the accurate control of local temperature at amplification chamber 9 can be realized after setting heating refrigeration component 18,
Other positions are not influenced.If certain environment temperature can satisfy PCR amplification requirement, without starting heating refrigeration component
18。
Further, as shown in Figure 3 and Figure 4, the upper layer chip 20 is located to be equipped in the amplification chamber 9 and extend downwardly
More miniature cylinders 21, the edge that the filter device 8 is located in the amplification chamber 9 is fixed by the miniature cylinder 21,
The remaining edge of the filter device 8 is pressed between the upper layer chip 20 and lower layer chip 19.In this way when upper layer chip 20 with
When lower layer chip 19 is spliced, any additional fixation device is not needed, so that it may fix edge everywhere in filter device 8, i.e.,
Fence and filter paper marginal portion are fixed on upper layer chip 20 and the interface of lower layer chip 19, filter paper out-of-flatness is avoided, is prevented
Only long chain DNA leakage during liquid flowing.
Preferably, as shown in figure 4,22 regions and the filter paper are circle at the cut-off, the circle of the filter paper
The heart is located at the cut-off 22 downstream, and the center of circle of the filter paper is located at the cut-off except 22 regions.Filter paper herein
For circle, need to guarantee the 22 whole symmetrical centre no more than filter paper at cut-off when installing.Amplification chamber 9 occupies filter paper
The space of top and downstream, 9 entirety of amplification chamber and third Micro-flow pipe downstream are respectively positioned on the bottom of upper layer chip 20
On face.
On the whole, the application mainly has the advantages that
1, whole reagents are stored on chip in liquid form, simplify operation；
2, the mode based on the filtering retention of filter device 8 carries out DNA extraction operation, and chip greatly simplifies, manufacturing cost drop
It is low；
3, the convenience of the validity and feed liquor that enable chip to take into account storage is designed by unique liquid storage structure.
Finally, it should be noted that above embodiments are only to illustrate the technical solution of the utility model, rather than its limitations；
Although the utility model is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that:
It is still possible to modify the technical solutions described in the foregoing embodiments, or part of technical characteristic is carried out etc.
With replacement；And these are modified or replaceed, various embodiments of the utility model technology that it does not separate the essence of the corresponding technical solution
The spirit and scope of scheme.
Claims (10)
1. a kind of extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that extract and expand including at least one DNA
Increasing unit, the DNA extracts amplification unit and includes: lysate storage pool, amplification system storage pool, expands chamber, filter device,
The lysate storage pool, amplification system storage pool pass through pipeline respectively and are connected to the entrance of the amplification chamber, the filtering
The inlet of the amplification chamber is arranged in device；The outlet of the amplification chamber is connected to pipe outlet by pipeline, described
Being communicated on pipeline between amplification chamber and pipe outlet closed takes liquid to export；The lysate storage pool, amplification body
It is that storage pool, pipe outlet three and the on off operating mode of the amplification chamber can switch respectively.
2. according to claim 1 extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that described to split
The outlet of solution liquid storage pool is connected with the first Micro-flow pipe, and first Micro-flow pipe is equipped with the first integrated chip valve；
The outlet of the amplification system storage pool is connected with the second Micro-flow pipe, and second Micro-flow pipe is equipped with the second chip
Pile-up valve；First Micro-flow pipe is connected to after converging with the second Micro-flow pipe with the first end of total pipeline, the general pipeline
The second end in road is connected to the entrance of the amplification chamber；Pass through the between the outlet and the pipe outlet of the amplification chamber
Three Micro-flow pipes connection, positioned at the outlet and the third Micro-flow pipe taken between liquid outlet of the amplification chamber
It is equipped with third integrated chip valve.
3. according to claim 2 extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that described to split
The connectivity part for solving liquid storage pool and first Micro-flow pipe is equipped with strainer.
4. according to claim 3 extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that the filter
Net surface contact angle is greater than 90 degree.
5. according to claim 2 extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that further include
Chip pipe main body and the chip liquid storing part being attached thereto, the lysate storage pool and amplification system storage pool are arranged at institute
It states in chip liquid storing part, the amplification chamber, the first Micro-flow pipe, the second Micro-flow pipe, the equal position of third Micro-flow pipe
In in the chip pipe main body, the first integrated chip valve, third integrated chip valve, takes liquid to go out at the second integrated chip valve
Mouth, pipe outlet are arranged on the chip pipe main body.
6. according to claim 5 extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that the core
Piece pipe main body is composed of upper layer chip with lower layer chip.
7. according to claim 6 extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that described total
Pipeline is the groove structure being arranged on the lower layer chip top surface, and the amplification chamber is to be arranged in the upper layer die bottom surface
On groove structure；After the upper layer chip is connect with lower layer chip, the amplification chamber is extended at the cut-off of the total pipeline
The lower section of room is to realize the connection with the amplification chamber；The filter device includes filter paper and is arranged around the edge of the filter paper
Fence, the filter paper level be laid at the cut-off with it is described amplification chamber connectivity part.
8. according to claim 1-7 extract amplification module based on micro-fluidic full-automatic DNA, feature exists
In equipped with heating refrigeration component at the amplification chamber.
9. according to claim 7 extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that on described
Layer chip, which is located in the amplification chamber, is equipped with the more miniature cylinders extended downwardly, and the filter device is located at the amplification chamber
Indoor edge is fixed by the miniature cylinder, and the remaining edge of the filter device is pressed in the upper layer chip and lower layer chip
Between.
10. according to claim 9 extract amplification module based on micro-fluidic full-automatic DNA, which is characterized in that described section
Only locate region and the filter paper is circle, the center of circle of the filter paper is located at the downstream at the cut-off, the filter paper
The center of circle is located at the cut-off except region.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820775734.0U CN208649284U (en) | 2018-05-23 | 2018-05-23 | It is a kind of that amplification module is extracted based on micro-fluidic full-automatic DNA |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820775734.0U CN208649284U (en) | 2018-05-23 | 2018-05-23 | It is a kind of that amplification module is extracted based on micro-fluidic full-automatic DNA |
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| CN208649284U true CN208649284U (en) | 2019-03-26 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111218395A (en) * | 2020-04-18 | 2020-06-02 | 博奥生物集团有限公司 | Full-flow biological detection device |
| CN113174308A (en) * | 2021-01-15 | 2021-07-27 | 北京中科生仪科技有限公司 | Purification amplification device |
| CN114308149A (en) * | 2021-11-29 | 2022-04-12 | 北京机械设备研究所 | Chip sealing device and self-sealing modular chip equipment |
| CN114507584A (en) * | 2022-01-26 | 2022-05-17 | 深圳市锦隆生物科技有限公司 | Liquid external driving device, method and equipment of biological micro-fluidic chip card box |
| WO2022174472A1 (en) * | 2021-02-19 | 2022-08-25 | 杭州梓晶生物有限公司 | Fully-integrated multi-index nucleic acid test microfluidic chip |
-
2018
- 2018-05-23 CN CN201820775734.0U patent/CN208649284U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111218395A (en) * | 2020-04-18 | 2020-06-02 | 博奥生物集团有限公司 | Full-flow biological detection device |
| CN111218395B (en) * | 2020-04-18 | 2020-08-07 | 博奥生物集团有限公司 | Full-flow biological detection device |
| CN113174308A (en) * | 2021-01-15 | 2021-07-27 | 北京中科生仪科技有限公司 | Purification amplification device |
| CN113174308B (en) * | 2021-01-15 | 2021-12-14 | 北京中科生仪科技有限公司 | Purification amplification device |
| WO2022174472A1 (en) * | 2021-02-19 | 2022-08-25 | 杭州梓晶生物有限公司 | Fully-integrated multi-index nucleic acid test microfluidic chip |
| CN114308149A (en) * | 2021-11-29 | 2022-04-12 | 北京机械设备研究所 | Chip sealing device and self-sealing modular chip equipment |
| CN114308149B (en) * | 2021-11-29 | 2024-03-01 | 北京机械设备研究所 | Chip sealing device and self-sealing modularized chip equipment |
| CN114507584A (en) * | 2022-01-26 | 2022-05-17 | 深圳市锦隆生物科技有限公司 | Liquid external driving device, method and equipment of biological micro-fluidic chip card box |
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