CN206951224U - A kind of micro-fluid chip in the experiment for WB - Google Patents
A kind of micro-fluid chip in the experiment for WB Download PDFInfo
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- CN206951224U CN206951224U CN201720699889.6U CN201720699889U CN206951224U CN 206951224 U CN206951224 U CN 206951224U CN 201720699889 U CN201720699889 U CN 201720699889U CN 206951224 U CN206951224 U CN 206951224U
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Abstract
The utility model discloses the micro-fluid chip in a kind of experiment for WB, including chip body, chip body includes upper cover plate, lower cover, the bottom of lower cover is fixed with magnetic stripe, the upper surface of lower cover is provided with the reaction chamber placed for slide glass film, after slide glass film is placed in reaction chamber, the lamination with magnetic stripe absorption is placed with slide glass film, sealing gasket is provided between upper cover plate and lower cover, the working hole communicated with reaction chamber is respectively equipped with the two side of lower cover, the sample introduction joint being connected with flow pump is plugged with working hole respectively and goes out sample joint, realized and be connected by clamp assembly between upper cover plate and lower cover;Experimental procedures can be simplified after having used this micro-fluid chip, lighten one's labor power, saves antibody dosage and cleaning fluid, improves stable experiment.
Description
Technical field
The present invention relates to the micro-fluid chip in Bioexperiment field, more particularly to a kind of experiment for WB.
Background technology
WB full name is WesternBlot immunoblot methods, also known as immunoblotting analysis, is to pass through the protein example of acquisition
SDS- polyacrylamide gel electrophoresises, the protein of different molecular weight is separated, and by electrophoretic blotting by gel points
From to protein be transferred to solid support(NC films or pvdf membrane)On, with the non-labeled antibody of anti-target protein(Primary antibody)With turning
Print caudacoria on target protein specifically bound, then with through horseradish peroxidase-labeled(Coupling)Two anti-bindings, finally
Detected with the super quick luminescent solution reagents of ECL.
In general WB experimentations are needed by multiple hand sampling, the plurality of step such as cleaning, machine concussion is carried out repeatedly,
The cumbersome of experimentation is caused, efficiency is low, and adopts and manually easily cause experimental error, so as to have impact on experiment
Resistance to overturning.
The content of the invention
It is an object of the invention to provide the micro-fluid chip in a kind of experiment for WB, it the advantage is that, can effectively carry
High conventional efficient and the stability of experiment.
The present invention above-mentioned technical purpose technical scheme is that:It is a kind of to be used in WB experiments
Micro-fluid chip, including chip body, the chip body include upper cover plate, lower cover, and the bottom of the lower cover is fixed with magnetic
Bar, the upper surface of the lower cover is provided with the reaction chamber placed for slide glass film, after slide glass film is placed in reaction chamber, the load
The lamination with magnetic stripe absorption is placed with piece film, sealing gasket is provided between the upper cover plate and lower cover, the lower cover
The working hole communicated with reaction chamber is respectively equipped with two side, the sample introduction being connected with flow pump is plugged with respectively on the working hole
Joint and go out sample joint, realized and be connected by clamp assembly between the upper cover plate and lower cover.
By using above-mentioned technical proposal, the slide glass film for being loaded with sample is placed in reaction chamber, and lamination is placed
Away from sample on slide glass film, lamination mutually adsorbs with magnetic stripe can ensure that slide glass film placement location in reaction chamber is steady
It is fixed firm, random position skew will not occur, afterwards upper cover plate is overlapped with lower cover, and realized by clamp assembly
Firm connection between cover plate and lower cover;Antibody is entered in reaction chamber by sample introduction joint and working hole to be carried out with response sample
Binding experiment, now due to the presence of sample introduction joint and working hole, the uninterrupted that antibody enters in reaction chamber is limited, from
And the microflow phenomena that antibody enters in reaction chamber can be realized, the flow process that the antibody of low discharge enters in reaction chamber can
Quickly sufficiently combined with response sample, avoid the step of later stage machine shakes, reduce experimental procedure, improve work effect
Rate, and effectively sample size can be controlled by the sample introduction of apparatus control antibody, reduce error, improve experiment
Stability, the presence of sealing gasket then enhance the sealing inside this micro-fluid chip, avoid the leakage of antibody in experiment, enter one
Step ensure that the stability of experiment.
The present invention is further arranged to:The both ends of the reaction chamber are equipped with the model groove communicated with reaction chamber, the mould
Type groove triangular shape.
By using above-mentioned technical proposal, model groove uses the design of triangle, and the oblique side wall of triangle is easy to liquid
Flowing, so as in cleaning of the later stage to reaction chamber, beneficial to the removing of cleaning fluid.
The present invention is further arranged to:The reaction chamber surface array is provided with multiple support columns.
By using above-mentioned technical proposal, because support column has gap each other, so that slide glass film exists
Placement on support column effectively avoids the absorption completely of slide glass film from reaction chamber surface, being easy to later stage slide glass film there is also gap
Disengaging.
The present invention is further arranged to:The upper cover plate is provided with multiple steam vents.
By using above-mentioned technical proposal, because in experimentation, the reaction chamber of this micro-fluid chip is air-tight state,
Now reaction chamber content is also easy to produce bubble, in the presence of steam vent, effectively prevent the phenomenon for occurring bubble in reaction chamber, enters
One step improves the stability of experiment.
Invention is further arranged to:The lower cover bottom is provided with glove groove, and the magnetic stripe card is embedding, and to be arranged on glove recessed
In groove.
, can be with relatively reduced micro-fluid chip after magnetic stripe is arranged in cover plate by using above-mentioned technical proposal
Overall space-consuming, and protective effect can be carried out to magnetic stripe, reduce the abrasion of magnetic stripe.
The present invention is further arranged to:The sample introduction joint and go out sample joint and realize and work respectively by silica gel tube head
The grafting in hole, the lower cover are made up of PMMA materials.
By using above-mentioned technical proposal, a setting can occur under pressure for lower cover made of PMMA materials
Become,, can be with after carrying out compression connection by clamp assembly between upper cover plate and lower cover after silica gel tube head grafting enters in working hole
So that working hole and silicone tube are first with deformation, fatigue cracking caused by working hole and silica gel tube head in Reusability is reduced
Phenomenon, extend the service life of this micro-fluid chip parts.
The present invention is further arranged to:The lamination outer surface is coated with anti-adherent coating.
By using above-mentioned technical proposal, the work of the positioning to slide glass film is being realized coated with the lamination after anti-adherent coating
With while, it is possible to reduce the suction-operated to antibody.
In summary, the invention has the advantages that:
1st, employ micro-fluid chip in WB experiments and can effectively antagonize body flow and be controlled, reduce experimental error, carry
The high stability of experiment;
2nd, experimental procedure can be simplified by micro-fluid chip, by complicated manual operation process with Machine automated completion,
Improve operating efficiency and labour can be saved;
3rd, the use of antibody and cleaning fluid can be saved in WB experiments by micro-fluid chip, has saved cost;
4th, in the design of micro-fluid chip by simple structure can slide glass film placed in reaction chamber stable, protect
Confirm that tests is smoothed out, and be convenient to mount and dismount, operation is fast and convenient;
5th, in micro-fluid chip model groove design can not influence experiment in the case of relatively reduced reaction chamber dead point
Position, it is easy to the removing work of later stage cleaning fluid;
6th, the design of support column can avoid slide glass film from being attached completely in reaction chamber in micro-fluid chip, be easy to slide glass film
Depart from from the dismounting on reaction chamber;
7th, the design of support column can increase reaction cavity space in micro-fluid chip, be easy to the abundant of antibody and response sample
With reference to, while also improve cleaning efficiency;
8th, the design of steam vent can further improve experiment with caused bubble in elimination reaction chamber in micro-fluid chip
Stability;
9th, in micro-fluid chip glove groove design can setting in order to magnetic stripe, so as to reduce this micro-fluid chip
Overall area occupied, protective effect also is carried out to magnetic stripe;
10th, the convenience during use is improved using a variety of simple structures in this micro-fluid chip, and extended
The service life of this micro-fluid chip.
Brief description of the drawings
Fig. 1 is the structural representation of this micro-fluid chip in embodiment 1;
Fig. 2 is the structural representation of glove groove in embodiment 1;
Fig. 3 is the structural representation of lower cover in embodiment 1;
Fig. 4 is the structural representation of upper cover plate in embodiment 2;
Fig. 5 is sample introduction joint and to go out the structural representation of sample joint in embodiment 3;
Fig. 6 is the structural representation of embodiment 4.
In figure:1st, upper cover plate;11st, steam vent;2nd, lower cover;21st, reaction chamber;211st, model groove;212nd, support column;22、
Working hole, 23, glove groove;3rd, magnetic stripe;4th, lamination;5th, sealing gasket;6th, sample introduction joint;7th, sample joint is gone out;8th, silica gel tube head;
91st, primary antibody sample introduction joint;92nd, secondary antibody sample introduction joint;93rd, primary antibody goes out sample joint;94th, secondary antibody goes out sample joint;95th, cleaning fluid import
Joint;96th, rinse liquid outlet joint.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing.
Embodiment 1:A kind of micro-fluid chip in the experiment for WB, as Figure 1-3, including chip body, chip body master
To include a upper cover plate 1 and a lower cover 2, the bottom of lower cover 2 is provided with a glove groove 23, in glove groove 23
Inlay card has a magnetic stripe 3, and a reaction chamber 21 is provided with the upper surface of lower cover 2, and the reaction chamber 21 is rectangle shape, and instead
Array on the surface of chamber 21 is answered to be fixed with multiple support columns 212.
After the slide glass film for being mounted with response sample is placed on the support column 212 in reaction chamber 21, lamination 4 is placed
Away from response sample on slide glass film, suction-operated of the magnetic stripe 3 to lamination 4 can realize slide glass film in support column 212
It is upper to place fastening, the fixation to position is produced, and the outer surface of this lamination 4 is coated with anti-adherent coating, such as by polytetrafluoro
Coating made of vinyl material, it can effectively reduce absorption of the lamination 4 to antibody.
As shown in the figures 1 and 2, afterwards between upper cover plate 1 and lower cover 2 add sealing gasket 5, the sealing gasket 5 be provided with it is anti-
The shape identical model groove of chamber 21 is answered, sealing gasket 5 can be realized in this micro-fluid chip by the way of two-sided glue sticking
Connection;Finally so that upper cover plate 1 carries out conjunction with lower cover 2 closes, and realized and be connected by clamp assembly, clamp assembly can
Pressurization fixation is carried out in a manner of being bolted or using electromagnetic field.
The working hole 22 communicated with reaction chamber 21 is respectively equipped with the both sides of lower cover 2, is also distinguished in the both sides of lower cover 2
Provided with the sample introduction joint 6 controlled by flow pump and go out sample joint 7, and in sample introduction joint 6 and go out sample joint 7 close to working hole 22
Place is equipped with the silica gel tube head 8 that can enter with grafting in working hole 22, from sample introduction joint 6 and goes out sample joint 7 for the ease of antibody
Interior circulation, sample introduction joint 6 and go out sample joint 7 be plugged on it is inclined during 2 both ends of lower cover.
Embodiment:The slide glass film for being loaded with response sample is placed on the support column 212 in reaction chamber 21, and led to
Cross lamination 4 and realize that slide glass film is fixed position in reaction chamber 21, load sealing gasket 5 afterwards, finally cause upper cover plate 1 and lower cover
Plate 2 is overlapped, and the firm connection between upper cover plate 1 and lower cover 2 is realized by clamp assembly.
By sample introduction joint 6, grafting enters in the working hole 22 of lower cover both sides respectively with sample joint 7 is gone out, and antibody passes through sample introduction joint
6 and working hole 22 enter reaction chamber 21 in response sample be combined experiment, now go out sample joint 7 and close;Due to flow pump
Control action, can be controlled to entering the antibody flow in reaction chamber 21, while in sample introduction joint 6 and working hole 22
In the presence of, antibody is further limit into the uninterrupted in reaction chamber 21, so as to realize that antibody enters reaction chamber
Microflow phenomena in 21, the antibody of low discharge can prolong slide glass film surface after entering in reaction chamber 21 and scatter, flow over herein
Quickly can be combined in journey with response sample, due to now enter in reaction chamber 21 amount of antibody that is combined with response sample compared with
It is few, so as to be easy to antibody sufficiently and the combination of response sample, Binding experiment is completed, the waste of antibody will not be caused.
After Binding experiment terminates, sample joint 7 is opened out, unnecessary antibody can flow out by going out sample joint 7, under being easy to
Secondary is continuing with, and further saves antibody, has saved cost.
When needing to test secondary antibody, it is necessary to being cleaned in reaction chamber 21, now cleaning fluid can also directly pass through
Sample introduction joint 6 enters in reaction chamber 21, and after realizing by its flow effect in reaction chamber 21 cleaning to reaction chamber 21
Flowed out again by going out sample joint 7, now micro-fluidic technologies can also save cleaning fluid when ensureing the cleaning performance to reaction chamber 21
Dosage.
In the present embodiment, antibody is by the flow control action of flow pump, even into reaction chamber 21, it is quick fully
Be combined experiment with response sample, eliminate later stage machine concussion, reduce experimental procedure, improve operating efficiency and reality
The stability tested.
In the present embodiment, as shown in figure 3, Making mold for the ease of this micro-fluid chip, the reaction chamber on lower lid
21 are arranged to rectangle, the model groove 211 communicated with reaction chamber 21 are now equipped with the both ends of reaction chamber 21, model groove 211 is then
Triangular shape, now the sealing end of model groove 211 of triangular shape instead of the sealing end of original both ends rectangle of reaction chamber 21,
So as to reduce the dead angle of sealing end, when being cleaned to reaction chamber 21, the model groove 211 of triangle is more favorable for cleaning
The cleaning work to the cleaning liquid inside of reaction chamber 21 is also further easy in the flowing of liquid, the reduction at dead angle.
In the present embodiment, as shown in figure 3, the support column 212 that array is arranged in reaction chamber 21 can enter to slide glass film
The uniform supporting role of row, while when slide glass film surface is stained with liquid, there is sky when slide glass film is placed on support column 212
Gap, the state attached completely will not be formed, consequently facilitating slide glass film takes out out of this micro-fluid chip, and taken out more light
Pine, i.e., certain protective effect is also played to slide glass film.
Above-mentioned slide glass film typically all carries many pores, and due to the presence of support column 212, slide glass film is being placed on into branch
After on dagger 212, the space of reaction chamber 21 can be increased, now antibody being flowed up and down from slide glass film, it is anti-to increase
The abundant combination of body and response sample, and in flushing process afterwards, can also similarly strengthen cleaning fluid with it is non-specific
With reference to more improve the later stage washes membrane efficiency.
In the present embodiment, as illustrated in fig. 1 and 2, the lower cover 2 of this micro-fluid chip is mainly made up of plastic material, such as
PMMA, PC, COC etc., so as to which Light deformation occurs under weight, reduce the fatigue damage of lower cover 2;When this microfluid core
When piece is completely embedded between the lower cover 2 of upper cover plate in the presence of clamp assembly 1, the working hole 22 of the both sides of lower cover 2 is arranged on
And consistent Light deformation can all occur under pressure for the silica gel tube head 8 that grafting enters in working hole 22, reduce fatigue cracking
Situation occur, the protective effect obtained using the characteristic of itself extends the service life of this micro-fluid chip, and saves
Cost.
Embodiment 2:A kind of micro-fluid chip in experiment for WB, is with the difference of embodiment 1, such as Fig. 4 institutes
Showing, the upper cover plate 1 of book micro-fluid chip is made up of transparent glass material, and multiple steam vents 11 are provided with upper cover plate 1,
The packet of steam vent 11 is arranged on the both sides opening position of upper cover plate 1, and corresponding with the position of the model groove 211 of lower cover 2, these exhausts
Hole 11 is completed by the technique of photoetching corrosion, to improve the precision of steam vent 11;The setting of steam vent 11 can be in order to reacting
The smooth discharge of gas in chamber 21, avoids occurring the scene of bubble in reaction chamber 21, further increases the stability of experiment.
Embodiment 3:A kind of micro-fluid chip in experiment for WB, is with the difference of embodiment 1, such as Fig. 5 institutes
Show, above-mentioned sample introduction joint 6 and go out sample joint 7 using Y types design, so as to increase inside circulation flow, be easy to compared with
Big flow and can smoothly it be conveyed compared with low discharge.
Embodiment 4:A kind of micro-fluid chip in experiment for WB, is with the difference of embodiment 1, such as Fig. 6 institutes
Show, when the sealing gasket 5 with model groove is arranged on inside this micro-fluid chip, its thickness can directly constitute this microfluid
The reaction chamber 21 of chip, the primary antibody sample introduction with being controlled by flow pump and being communicated with reaction chamber 21 is respectively arranged with upper cover plate 1
Joint 916, secondary antibody sample introduction joint 926 and cleaning fluid inlet union 95, on lower cover 2 are correspondingly provided with primary antibody and go out sample and connect respectively
First 937, secondary antibody goes out sample joint 947 and rinse liquid outlet joint 96, similar with the course of work described in embodiment 1, will be loaded with
The slide glass film of response sample causes slide glass film in reaction chamber 21 in reaction chamber 21 using the elastic deformation force of sealing gasket 5
Inside set firmly, entered respectively by primary antibody sample introduction joint 916, secondary antibody sample introduction joint 926 and cleaning fluid inlet union 95 afterwards
Traveling resists and the work of cleaning, goes out sample joint 937 by primary antibody, secondary antibody goes out sample joint 947 and rinse liquid outlet joint 96
The row of progress resists and the work of cleaning fluid, on the premise of it can smoothly realize Binding experiment and cleaning, this microfluid
The disengaging of the antibody and cleaning fluid of chip has obtained preferable differentiation, will not cause both mixing, is easy to antibody and cleaning
The recycling of liquid.
This specific embodiment is only explanation of the invention, and it is not limitation of the present invention, people in the art
Member can make the modification of no creative contribution to the present embodiment as needed after this specification is read, but as long as at this
All protected in the right of invention by Patent Law.
Claims (7)
1. the micro-fluid chip in a kind of experiment for WB, including chip body, it is characterised in that:The chip body includes upper cover plate
(1), lower cover (2), the bottom of the lower cover (2) are fixed with magnetic stripe (3), and the upper surface of the lower cover (2), which is provided with, to be supplied
The reaction chamber (21) that slide glass film is placed, when slide glass film be placed on reaction chamber (21) it is interior after, be placed with the slide glass film and magnetic stripe
(3) lamination (4) of absorption, is provided with sealing gasket (5) between the upper cover plate (1) and lower cover (2), the lower cover (2)
The working hole (22) communicated with reaction chamber (21) is respectively equipped with two side, is plugged with respectively on the working hole (22) and flow
The sample introduction joint (6) and go out sample joint (7) that pump connects, are realized between the upper cover plate (1) and lower cover (2) by clamp assembly
It is connected.
2. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that:The reaction chamber
(21) both ends are equipped with the model groove (211) communicated with reaction chamber (21), model groove (211) triangular shape.
3. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that:The reaction chamber
(21) surface array is provided with multiple support columns (212).
4. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that:The upper cover plate
(1) multiple steam vents (11) are provided with.
5. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that:The lower cover
(2) bottom is provided with glove groove (23), and magnetic stripe (3) inlay card is arranged in glove groove (23).
6. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that:The sample introduction joint
(6) and go out sample joint (7) and realized respectively by silica gel tube head (8) and working hole(22)Grafting, the lower cover (2) by
PMMA materials are made.
7. the micro-fluid chip in a kind of experiment for WB according to claim 1, it is characterised in that:The lamination
(4) outer surface is coated with anti-adherent coating.
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CN109126911A (en) * | 2017-06-15 | 2019-01-04 | 南京巴傲得生物科技有限公司 | A kind of micro-fluid chip in WB experiment |
CN109731620A (en) * | 2018-12-29 | 2019-05-10 | 哈尔滨工业大学 | A kind of Pneumatic horizontal micro-fluidic biological biomedical chip with transition cavity |
CN110387321A (en) * | 2018-04-23 | 2019-10-29 | 深圳华大智造科技有限公司 | Gene sequencing chip and gene sequencing device |
CN110479393A (en) * | 2019-08-24 | 2019-11-22 | 德运康明(厦门)生物科技有限公司 | A kind of programmable micro-fluidic chip surface modification of and coloration instrument |
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2017
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109126911A (en) * | 2017-06-15 | 2019-01-04 | 南京巴傲得生物科技有限公司 | A kind of micro-fluid chip in WB experiment |
CN110387321A (en) * | 2018-04-23 | 2019-10-29 | 深圳华大智造科技有限公司 | Gene sequencing chip and gene sequencing device |
CN110387321B (en) * | 2018-04-23 | 2023-03-17 | 深圳华大智造科技股份有限公司 | Gene sequencing chip and gene sequencing device |
CN109731620A (en) * | 2018-12-29 | 2019-05-10 | 哈尔滨工业大学 | A kind of Pneumatic horizontal micro-fluidic biological biomedical chip with transition cavity |
CN109731620B (en) * | 2018-12-29 | 2021-01-12 | 哈尔滨工业大学 | Pneumatic horizontal micro-fluidic biomedical chip with transition cavity |
CN110479393A (en) * | 2019-08-24 | 2019-11-22 | 德运康明(厦门)生物科技有限公司 | A kind of programmable micro-fluidic chip surface modification of and coloration instrument |
CN110479393B (en) * | 2019-08-24 | 2021-11-12 | 苏州德运康瑞生物科技有限公司 | Programmable micro-fluidic chip surface modification dyeing instrument |
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Effective date of registration: 20230111 Address after: No. 251, Phase 1, No. 926, Changhong East Street, Fuxi Street, Deqing County, Huzhou City, Zhejiang Province (Moganshan National High-tech Zone) Patentee after: Zhejiang Comparex Biotechnology Co.,Ltd. Address before: Room 110, Building F6, No. 9, Weidi Road, Xianlin University Town, Xianlin Street, Qixia District, Nanjing City, Jiangsu Province, 210036 Patentee before: NANJING BIOGOT BIOTECHNOLOGY CO.,LTD. |
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