CN110479393B - Programmable micro-fluidic chip surface modification dyeing instrument - Google Patents
Programmable micro-fluidic chip surface modification dyeing instrument Download PDFInfo
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- CN110479393B CN110479393B CN201910786261.3A CN201910786261A CN110479393B CN 110479393 B CN110479393 B CN 110479393B CN 201910786261 A CN201910786261 A CN 201910786261A CN 110479393 B CN110479393 B CN 110479393B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
Abstract
The invention discloses a programmable micro-fluidic chip surface modification dyeing instrument, and relates to the technical field of micro-fluidic chips. The technical key points are as follows: a programmable micro-fluidic chip surface modification dyeing instrument comprises a shell, wherein a fixed plate is arranged on the shell, at least one chip groove is formed in the fixed plate, a first liquid inlet joint, a second liquid inlet joint, a first liquid outlet joint and a second liquid outlet joint which are communicated with a chip are arranged at two ends of the chip groove, and a connecting line of the first liquid inlet joint and the first liquid outlet joint is crossed with a connecting line of the second liquid inlet joint and the second liquid outlet joint; a pressing component for pressing the chip is arranged on the fixing plate; the shell is provided with at least one fluid connector, and a control assembly for controlling liquid flow is arranged in the shell. The invention has the advantages of uniform reagent distribution, high efficiency and small batch production difference.
Description
Technical Field
The invention relates to the technical field of microfluidic chips, in particular to a programmable microfluidic chip surface modification dyeing instrument.
Background
The micro-fluidic chip generally comprises a substrate and a structure, wherein the substrate is usually made of glass and silicon chip materials, the structure is mostly a micro-scale fluid channel (namely a micro-channel) built by high polymer materials (such as PDMS, PMMA, PC and the like), and the surface of the micro-fluidic chip is effectively modified due to high hydrophobicity, so that the micro-fluidic chip has a reactive functional group and an affinity ligand, and the function and application field of the micro-fluidic chip are determined. The modified chip can be used for sample analysis in the fields of chemistry, biology, medicine and the like, and the target substance is always required to be dyed and identified after staying in the chip so as to analyze the object to be detected, so that the uniform and efficient dyeing is very important for accurate judgment of the sample.
In the traditional micro-fluidic chip modification/dyeing mode, a modification/dyeing reagent needs to be injected from a micro-channel sample inlet by a handheld injector, on one hand, because the flow and the flow velocity are difficult to accurately control during manual injection, most of the reagent is easily distributed in a local area after entering a micro-channel, and the reagent is unevenly distributed; on the other hand, the manual operation efficiency is low, and unstable factors exist, so that the micro-fluidic chips in different batches have batch-to-batch difference, and the judgment of experimental results is influenced.
Disclosure of Invention
The invention aims to provide a programmable micro-fluidic chip surface modification dyeing instrument which has the advantages of uniform reagent distribution, high efficiency and small batch production difference.
In order to achieve the purpose, the invention provides the following technical scheme:
a programmable micro-fluidic chip surface modification dyeing instrument comprises a shell, wherein a fixed plate is arranged on the shell, at least one chip groove is formed in the fixed plate, a first liquid inlet joint, a second liquid inlet joint, a first liquid outlet joint and a second liquid outlet joint which are communicated with a chip are arranged at two ends of the chip groove, and a connecting line of the first liquid inlet joint and the first liquid outlet joint is crossed with a connecting line of the second liquid inlet joint and the second liquid outlet joint;
a pressing component for pressing the chip is arranged on the fixing plate;
the shell is provided with at least one fluid connector, and a control assembly for controlling liquid flow is arranged in the shell.
By adopting the technical scheme, when surface modification is carried out, the chip is placed in the chip groove, the liquid inlet hole and the liquid outlet hole of the chip are respectively communicated with the first liquid inlet joint, the second liquid inlet joint, the first liquid outlet joint and the second liquid outlet joint, the chip is tightly pressed in the chip groove by the pressing component, the control component controls 50% of reagent in volume to enter a micro-channel in the chip from the first liquid inlet joint and flow out from the second liquid inlet joint, and at the moment, the second liquid inlet joint and the second liquid outlet joint are in a closed state; then close first feed liquor joint and second feed liquor joint, open second feed liquor joint and second and go out liquid joint, 50% volume's reagent gets into the inside microchannel of chip from the second feed liquor joint, goes out liquid joint outflow from the second again. Because the connecting line of the first liquid inlet joint and the first liquid outlet joint is crossed with the connecting line of the second liquid inlet joint and the second liquid outlet joint, the reagent is more easily and uniformly distributed when flowing twice; according to the needs, a plurality of chips can be modified at one time, so that the efficiency is high; the horizontal micro-fluidic injection pump can be adopted for injection, manual injection is not needed, and batch production is not different.
Further, the chip grooves are at least arranged in two, a first channel communicated with a first liquid outlet joint and a first liquid inlet joint of the adjacent chip grooves is formed in the fixing plate, and a second channel communicated with a second liquid outlet joint and a second liquid inlet joint of the adjacent chip grooves is formed in the fixing plate.
By adopting the technical scheme, a plurality of chips can be placed at the same time, and the plurality of chips can be modified at the same time through the first channel and the second channel, so that the efficiency is improved.
Furthermore, the compressing assembly comprises a pressing plate hinged to one side of the fixing plate, the other end of the pressing plate is connected with the fixing plate in a clamped mode, an observation hole is formed in the middle of the pressing plate, a pressing frame hinged to the pressing plate is arranged at the clamping end of the pressing plate, and the other end of the pressing frame is connected with the fixing plate in a clamped mode.
By adopting the technical scheme, when in installation, the chip is placed in the chip groove, the pressing plate is rotated to enable the clamping end to be clamped with the fixed plate, then the pressing frame is rotated, and the other end of the pressing frame is clamped with the fixed plate, so that the chip is fixed in the chip groove, and the installation is convenient; when the chip is disassembled, the pressing frame is applied with force and is rotated, and then the pressing plate is pulled up, so that the chip is released from being fixed, the disassembly is convenient, and the efficiency is improved.
Furthermore, one side of the fixed plate facing the pressing plate is provided with a holding tank, a rotating shaft connected with the fixed plate in a rotating mode is arranged in the holding tank, a semicircular arc-shaped plate is arranged on one side of the pressing plate facing the rotating shaft, and an opening of the arc-shaped plate faces the rotating shaft.
Through adopting above-mentioned technical scheme, behind the installation chip, the arc is located the below of pivot, receives the restriction of pivot, when the mistake is met and is pressed the frame and make it not hard up, the clamp plate still sticiss the chip, only when pressing the frame to rotate to the arc not receive the spacing of pivot, just can remove the fixed to the chip, the arc plays the effect that prevents the maloperation.
Furthermore, the upper surfaces of the two edges of the fixing plate facing one side of the pressing plate are provided with taking grooves.
Through adopting above-mentioned technical scheme, after opening the clamp plate, the groove of taking can provide the position of taking or placing the chip for the finger.
Furthermore, the side wall of one end, connected with the fixed plate in a clamping manner, of the pressing frame is provided with a shifting block.
Through adopting above-mentioned technical scheme, when dismantling, exert ascending power for the shifting block to rotate and press the frame, conveniently dismantle.
Furthermore, the control assembly comprises a multi-way valve communicated with a fluid connector, a connecting pipe is communicated between the fluid connector and the multi-way valve, a first liquid inlet pipe is communicated between the first liquid inlet connector and the multi-way valve, and a first liquid outlet pipe is communicated with the first liquid outlet connector;
the multi-way valve is characterized in that a second liquid inlet pipe is communicated between the second liquid inlet joint and the multi-way valve, a second liquid outlet pipe is communicated with the second liquid outlet joint, two-position two-way electromagnetic valves for controlling the switches of the first liquid outlet pipe and the second liquid outlet pipe are arranged on the peripheral sides of the first liquid outlet pipe and the second liquid outlet pipe, one ends, far away from the fixed plate, of the first liquid outlet pipe and the second liquid outlet pipe are communicated with a liquid outlet main pipe, and shunt electromagnetic valves for respectively controlling the switches of the connecting pipe, the first liquid inlet pipe and the second liquid inlet pipe are arranged on the multi-way valve.
By adopting the technical scheme, when modification is carried out, the first liquid inlet pipe and the first liquid outlet pipe are in a communicated state, the second liquid inlet pipe and the second liquid outlet pipe are in a closed state, 50% of reagent in volume enters the multi-way valve from the fluid connector through the connecting pipe, enters the microchannel in the chip through the first liquid inlet pipe, flows to the first liquid outlet pipe, and is finally discharged from the liquid outlet main pipe; at the moment, the first liquid inlet pipe and the first liquid outlet pipe are closed, the second liquid inlet pipe and the second liquid outlet pipe are opened, 50% of reagent in volume enters the multi-way valve from the fluid connector through the connecting pipe, then enters the micro-channel in the chip through the second liquid inlet pipe, flows to the second liquid outlet pipe, and finally is discharged from the liquid outlet main pipe, and the flow paths of the reagent twice are crossed, so that the reagent is more easily and uniformly distributed.
Furthermore, the side wall of the shell is provided with a clamping assembly clamped with the microfluidic injection pump.
By adopting the technical scheme, the micro-fluidic injection pump can be used together with the micro-fluidic injection pump, and the clamping assembly can connect the shell with the micro-fluidic injection pump, so that the shell is prevented from shaking, and the stability during modification is improved.
Compared with the prior art, the invention has the beneficial effects that:
(1) by arranging the first liquid inlet joint, the second liquid inlet joint, the first liquid outlet joint and the second liquid outlet joint, because the connecting line of the first liquid inlet joint and the first liquid outlet joint is crossed with the connecting line of the second liquid inlet joint and the second liquid outlet joint, reagents are easier to be uniformly distributed when flowing twice;
(2) by arranging the chip grooves, the first channel and the second channel, a plurality of chips can be placed at the same time, and the chips can be decorated at the same time through the first channel and the second channel, so that the efficiency is improved;
(3) through setting up the control assembly who controls liquid flow, can adopt horizontal micro-fluidic syringe pump to inject, control assembly control liquid flow path and order, do not need artifical injection, batch production difference is little.
Drawings
FIG. 1 is an overall schematic view of the present invention;
FIG. 2 is a top view of the present invention;
FIG. 3 is an expanded view of the fixation plate and compression assembly of the present invention;
FIG. 4 is a cross-sectional view of a mounting plate of the present invention taken along the plane of the first and second channels;
FIG. 5 is a side view of the fixation plate and hold-down assembly of the present invention in an expanded state;
FIG. 6 is a schematic view of the internal structure of the hidden housing according to the present invention;
FIG. 7 is a cross-sectional view of the multi-way valve and the shunt solenoid valve of the present invention;
fig. 8 is a perspective view of the present invention in use with a microfluidic syringe pump.
Reference numerals: 1. a housing; 2. a fixing plate; 3. a chip slot; 4. a first liquid inlet joint; 5. a second liquid inlet joint; 6. a first liquid outlet joint; 7. a second liquid outlet joint; 8. a fluid connector; 9. a first channel; 10. a second channel; 11. pressing a plate; 12. a microfluidic syringe pump; 13. an observation hole; 14. pressing the frame; 15. accommodating grooves; 16. a rotating shaft; 17. an arc-shaped plate; 18. taking the groove; 19. shifting blocks; 20. a multi-way valve; 21. a connecting pipe; 22. a first liquid inlet pipe; 23. a first liquid outlet pipe; 24. a second liquid inlet pipe; 25. a second liquid outlet pipe; 26. a two-position two-way solenoid valve; 27. a liquid outlet main pipe; 28. a shunt solenoid valve; 29. a clamping assembly; 30. a plug; 31. and a flow passage.
Detailed Description
The invention is described in detail below with reference to the figures and examples.
As shown in fig. 1, a programmable microfluidic chip surface modification dyeing instrument comprises a rectangular housing 1, wherein a clamping component 29 clamped with a microfluidic injection pump 12 (see fig. 8) is arranged on the side wall of the housing 1, and the clamping component 29 is a quick buckle. The clamping assembly 29 can connect the shell 1 with the microfluidic injection pump 12, so that the shell 1 is prevented from shaking, and the stability during modification is improved.
As shown in fig. 2 and fig. 3, a rectangular fixing plate 2 is disposed on the upper surface of a housing 1, at least one chip slot 3 for placing a chip is disposed on the fixing plate 2, in this embodiment, 6 chip slots 3 are disposed, a first liquid inlet connector 4, a second liquid inlet connector 5, and a first liquid outlet connector 6 are disposed at two ends of each chip slot 3 and communicated with the chip, and a second liquid outlet joint 7, wherein the first liquid inlet joint 4 and the second liquid inlet joint 5 are arranged at one end of the chip groove 3, the first liquid outlet joint 6 and the second liquid outlet joint 7 are arranged at the other end of the chip groove 3, a connecting line of the first liquid inlet joint 4 and the first liquid outlet joint 6 is crossed with a connecting line of the second liquid inlet joint 5 and the second liquid outlet joint 7, and solid plugs 30 for plugging two holes in the middle of the chip are respectively arranged between the first liquid inlet joint 4 and the second liquid inlet joint 5 and between the first liquid outlet joint 6 and the second liquid outlet joint 7.
As shown in fig. 3 and 4, the fixing plate 2 is provided with a first channel 9 for communicating the first liquid outlet joint 6 and the first liquid inlet joint 4 of the adjacent chip grooves 3, the fixing plate 2 is provided with a second channel 10 for communicating the second liquid outlet joint 7 and the second liquid inlet joint 5 of the adjacent chip grooves 3, and the flow path of the reagent is S-shaped. A plurality of chips can be decorated simultaneously through the first channel 9 and the second channel 10, and the efficiency is improved.
As shown in fig. 3 and 5, in order to fix the chip on the fixing plate 2, a pressing assembly for pressing the chip is arranged on the fixing plate 2, the pressing assembly includes a pressing plate 11 hinged to one side of the fixing plate 2, the other end of the pressing plate 11 is clamped to the fixing plate 2, an observation hole 13 is formed in the middle of the pressing plate 11, a pressing frame 14 hinged to the pressing plate 11 is arranged at the clamping end of the pressing plate 11, and the other end of the pressing frame 14 is elastically clamped to the fixing plate 2. During installation, a chip is placed in the chip groove 3, the pressing plate 11 is rotated to enable the clamping end of the pressing plate to be clamped with the fixing plate 2, the pressing frame 14 is rotated, the other end of the pressing frame 14 is clamped with the fixing plate 2, and therefore the chip is fixed in the chip groove 3, and installation is convenient. The upper surfaces of two edges of the fixing plate 2 facing to the pressing plate 11 are provided with taking grooves 18, and after the pressing plate 11 is opened, the taking grooves 18 can provide positions for taking or placing chips for fingers.
One side that fixed plate 2 faced clamp plate 11 is equipped with holding tank 15, is equipped with in the holding tank 15 and rotates the pivot 16 of being connected with fixed plate 2, and clamp plate 11 is provided with half-circular arc's arc 17 towards pivot 16 one side, and the opening of arc 17 is towards pivot 16. Only when the pressing frame 14 rotates to the arc-shaped plate 17 and is not limited by the rotating shaft 16, the chip can be released from being fixed, and the arc-shaped plate 17 plays a role in preventing misoperation.
The side wall integrated into one piece of pressing frame 14 and fixed plate 2 joint one end has shifting block 19, and during the dismantlement, applys ascending power for shifting block 19 to rotate pressing frame 14, convenient dismantlement.
As shown in fig. 2 and 6, at least one fluid connector 8 is disposed on the housing 1, in this embodiment, 6 fluid connectors 8 are disposed, and a control assembly for controlling the flow of the liquid is disposed inside the housing 1. The control assembly comprises a multi-way valve 20 communicated with the fluid connector 8, 8 mutually communicated radial flow passages 31 (see fig. 7) are formed in the multi-way valve 20, and connecting pipes 21 are communicated between the fluid connector 8 and the multi-way valve 20, wherein 6 connecting pipes 21 are arranged in the embodiment. A first liquid inlet pipe 22 is communicated between the first liquid inlet joint 4 and the multi-way valve 20, and a first liquid outlet pipe 23 is communicated between the first liquid outlet joint 6; a second liquid inlet pipe 24 is communicated between the second liquid inlet joint 5 and the multi-way valve 20, a second liquid outlet joint 7 is communicated with a second liquid outlet pipe 25, two-position two-way electromagnetic valves 26 for controlling the on and off of the first liquid outlet pipe 23 and the second liquid outlet pipe 25 are arranged on the peripheral sides of the first liquid outlet pipe 23 and the second liquid outlet pipe 25, one ends, far away from the fixed plate 2, of the first liquid outlet pipe 23 and the second liquid outlet pipe 25 are communicated with a liquid outlet header pipe 27, and a shunt electromagnetic valve 28 for respectively controlling the on and off of the connecting pipe 21, the first liquid inlet pipe 22 and the second liquid inlet pipe 24 is arranged on the multi-way valve 20.
It is worth to say that the surface modification instrument of the invention can be used for injecting sample into the chip after surface modification and dyeing the target objects adsorbed in the chip.
The working process and the beneficial effects of the invention are as follows:
when surface modification is carried out, a chip is placed in a chip groove 3, a liquid inlet hole and a liquid outlet hole of the chip are respectively communicated with a first liquid inlet joint 4, a second liquid inlet joint 5, a first liquid outlet joint 6 and a second liquid outlet joint 7, the chip is tightly pressed in the chip groove 3 by a pressing component, a first liquid inlet pipe 22 and a first liquid outlet pipe 23 are in a communicated state, a second liquid inlet pipe 24 and a second liquid outlet pipe 25 are in a closed state, 50% of reagent in volume enters a multi-way valve 20 through a fluid joint 8 and a connecting pipe 21, enters a micro-channel in the chip through the first liquid inlet pipe 22, flows to the first liquid outlet pipe 23, and is finally discharged from a liquid outlet header pipe 27; at this time, the first liquid inlet pipe 22 and the first liquid outlet pipe 23 are closed, the second liquid inlet pipe 24 and the second liquid outlet pipe 25 are opened, 50% of reagent in volume enters the multi-way valve 20 from the fluid connector 8 through the connecting pipe 21, enters the micro-channel in the chip through the second liquid inlet pipe 24, flows to the second liquid outlet pipe 25, and is finally discharged from the liquid outlet header pipe 27, the flow paths of the reagent twice are crossed, the reagent is more easily and uniformly distributed, a plurality of chips can be modified at one time, and the efficiency is high; the horizontal micro-fluidic injection pump 12 can be used for injection, manual injection is not needed, and batch production is not different.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (6)
1. A programmable micro-fluidic chip surface modification dyeing instrument comprises a shell (1) and is characterized in that a fixing plate (2) is arranged on the shell (1), at least one chip groove (3) is formed in the fixing plate (2), a first liquid inlet joint (4), a second liquid inlet joint (5), a first liquid outlet joint (6) and a second liquid outlet joint (7) which are communicated with a chip are arranged at two ends of the chip groove (3), and a connecting line of the first liquid inlet joint (4) and the first liquid outlet joint (6) is crossed with a connecting line of the second liquid inlet joint (5) and the second liquid outlet joint (7);
the number of the chip grooves (3) is at least two, a first liquid outlet joint (6) and a first channel (9) of a first liquid inlet joint (4) which are communicated with adjacent chip grooves (3) are arranged on the fixing plate (2), and a second liquid outlet joint (7) and a second channel (10) of a second liquid inlet joint (5) which are communicated with adjacent chip grooves (3) are arranged on the fixing plate (2);
a pressing component for pressing the chip is arranged on the fixing plate (2);
the shell (1) is provided with at least one fluid connector (8), and a control assembly for controlling liquid flow is arranged in the shell (1); the control assembly comprises a multi-way valve (20) communicated with a fluid connector (8), a connecting pipe (21) is communicated between the fluid connector (8) and the multi-way valve (20), a first liquid inlet pipe (22) is communicated between the first liquid inlet connector (4) and the multi-way valve (20), and a first liquid outlet connector (6) is communicated with a first liquid outlet pipe (23);
a second liquid inlet pipe (24) is communicated between the second liquid inlet joint (5) and the multi-way valve (20), the second liquid outlet joint (7) is communicated with a second liquid outlet pipe (25), two-position two-way electromagnetic valves (26) for controlling the on and off of the first liquid outlet pipe (23) and the second liquid outlet pipe (25) are arranged on the peripheral sides of the first liquid outlet pipe (23) and the second liquid outlet pipe (25), a liquid outlet header pipe (27) is communicated with one ends of the first liquid outlet pipe (23) and the second liquid outlet pipe (25) far away from the fixed plate (2), and a shunt electromagnetic valve (28) for respectively controlling the on and off of the connecting pipe (21), the first liquid inlet pipe (22) and the second liquid inlet pipe (24) is arranged on the multi-way valve (20);
a first liquid inlet joint (4) and a second liquid inlet joint (5) are arranged at one end of the chip groove (3), a first liquid outlet joint (6) and a second liquid outlet joint (7) are arranged at the other end of the chip groove (3), and a first liquid inlet pipe (22) and a second liquid inlet pipe (24) are positioned at different sides of the fixing plate (2);
when the chip works, the control assembly controls a reagent to enter a micro-channel in the chip from the first liquid inlet joint (4) and flow out from the first liquid outlet joint (6), and at the moment, the second liquid inlet joint (5) and the second liquid outlet joint (7) are in a closed state; then, the first liquid inlet joint (4) and the first liquid outlet joint (6) are closed, the second liquid inlet joint (5) and the second liquid outlet joint (7) are opened, and the reagent enters the micro-channel inside the chip from the second liquid inlet joint (5) and then flows out from the second liquid outlet joint (7).
2. The programmable micro-fluidic chip surface modification dyeing instrument according to claim 1, characterized in that the compressing assembly comprises a pressing plate (11) hinged to one side of the fixing plate (2), the other end of the pressing plate (11) is clamped to the fixing plate (2), an observation hole (13) is formed in the middle of the pressing plate (11), a pressing frame (14) hinged to the pressing plate (11) is arranged at the clamping end of the pressing plate (11), and the other end of the pressing frame (14) is clamped to the fixing plate (2).
3. The programmable micro-fluidic chip surface modification dyeing instrument according to claim 2, characterized in that a holding groove (15) is provided on a side of the fixing plate (2) facing the pressing plate (11), a rotating shaft (16) rotatably connected with the fixing plate (2) is provided in the holding groove (15), a semi-circular arc-shaped plate (17) is provided on a side of the pressing plate (11) facing the rotating shaft (16), and an opening of the arc-shaped plate (17) faces the rotating shaft (16).
4. The programmable micro-fluidic chip surface modification dyeing instrument according to claim 2, characterized in that the upper surfaces of two edges of the fixing plate (2) facing the pressing plate (11) are provided with taking grooves (18).
5. The programmable micro-fluidic chip surface modification dyeing instrument according to claim 2, characterized in that the side wall of the clamping end of the pressing frame (14) and the fixing plate (2) is provided with a shifting block (19).
6. The programmable microfluidic chip surface modification staining instrument of claim 1, wherein the side wall of the housing (1) is provided with a clamping component (29) clamped with the microfluidic syringe pump (12).
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| CN201910786261.3A CN110479393B (en) | 2019-08-24 | 2019-08-24 | Programmable micro-fluidic chip surface modification dyeing instrument |
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| CN109569755A (en) * | 2019-01-15 | 2019-04-05 | 苏州汶颢微流控技术股份有限公司 | Drop mass production device |
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