CN109731620B - Pneumatic horizontal micro-fluidic biomedical chip with transition cavity - Google Patents
Pneumatic horizontal micro-fluidic biomedical chip with transition cavity Download PDFInfo
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Abstract
The invention discloses a pneumatic horizontal microfluidic biomedical chip with a transition cavity, which consists of a liquid inlet layer, a transition layer, a reaction cavity layer, a liquid transfer layer, a film layer and a pneumatic control layer; the liquid inlet layer is distributed with a liquid inlet hole, an air inlet hole and a liquid inlet channel; the transition layer is provided with a transition cavity, a transition layer liquid inlet hole and a transition layer air inlet hole; the reaction cavity layer is distributed with a reaction cavity, a liquid inlet hole of the reaction cavity layer and an air inlet hole of the reaction cavity layer; the liquid transfer layer is provided with a liquid transfer layer reaction cavity, a liquid transfer layer air inlet hole, a liquid discharge flow channel and a transfer flow channel; the film layer is distributed with film layer liquid discharge holes and film layer air inlet holes; the pneumatic control layer is distributed with a pneumatic control layer liquid discharge hole, a pneumatic control layer air inlet flow channel and a pneumatic control cavity. The invention realizes the liquid inlet, liquid discharge, liquid transfer and the on-off control of the corresponding liquid flow channel by pneumatic control, thereby realizing the automation and integration of the reagent conveying and sample extracting processes in the microfluidic chip.
Description
Technical Field
The invention belongs to the technical field of microfluidic chips, and relates to a pneumatic horizontal microfluidic biomedical chip with a transition cavity.
Background
For the processes of clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microorganism detection, food safety detection, molecular biology analysis and the like, because of a plurality of operation steps, the whole analysis and treatment process needs to be completed manually, a large amount of manpower and time are consumed, and the cost of equipment and a kit is extremely high although the manpower is saved by an automatic workstation, so that the microfluidic technology gradually becomes an important means in the field of biomedical analysis and monitoring.
Microfluidic chips are a technology that integrates a series of operation units involved in the fields of chemistry, biology, and the like, including sample preparation, pretreatment, reaction, separation, analysis, and cell culture, sorting, lysis, and the like, into chips of a centimeter squared size or smaller. The micro-fluidic chip controls the on-off of the liquid micro-channel and the sample delivery, and has the characteristics of easy preparation, simple control mode, low cost and easy realization of large-scale integration, thereby replacing the prior manual operation, realizing automatic control and avoiding cross contamination.
Disclosure of Invention
The invention provides a pneumatic horizontal microfluidic biomedical chip with a transition cavity aiming at the requirements of the automation of biomedical analysis and detection processes and realizing the steps of sequential reagent liquid inlet, mixing, liquid transfer, liquid discharge, final sample extraction and the like. The invention utilizes PDMS, organic glass and other materials to carry out encapsulation in a soft etching mode, realizes liquid inlet, liquid discharge and liquid transfer control in an air pressure driving mode, and utilizes the change of air pressure in the pneumatic micro-channel to drive a film between the pneumatic micro-channel and the liquid micro-channel to deform so as to form the control micro-valve.
The purpose of the invention is realized by the following technical scheme:
a pneumatic horizontal microfluidic biomedical chip with a transition cavity is of a multilayer structure and comprises a liquid inlet layer, a transition layer, a reaction cavity layer, a liquid transfer layer, a film layer and a pneumatic control layer;
the liquid inlet layer is distributed with a liquid inlet hole and an air inlet hole, and one side of the liquid inlet layer sealed with the transition layer is distributed with a liquid inlet flow channel;
the transition layer is provided with a transition cavity, a transition layer liquid inlet hole and a transition layer air inlet hole;
the reaction cavity layer is provided with a reaction cavity, a reaction cavity layer liquid inlet hole and a reaction cavity layer air inlet hole;
the liquid transfer layer is provided with a liquid transfer layer reaction cavity, a liquid transfer cavity and a liquid transfer layer air inlet, and one side of the liquid transfer layer sealed with the thin film layer is provided with a liquid discharge flow channel and a transfer flow channel;
the film layer is distributed with film layer liquid discharge holes and film layer air inlet holes;
the pneumatic control layer is distributed with pneumatic control layer liquid discharge holes, and one side of the pneumatic control layer sealed with the thin film layer is distributed with a pneumatic control layer air inlet channel and a pneumatic control cavity;
the liquid inlet holes and the air inlet holes are distributed on one side or two sides of the liquid inlet layer;
one end of the liquid inlet flow channel is communicated with a liquid inlet hole, and the other end of the liquid inlet flow channel is respectively communicated with the transition cavity and the transition layer liquid inlet hole;
the transition cavity is communicated with the reaction cavity and the pipetting layer reaction cavity;
the transition layer liquid inlet hole is communicated with the reaction cavity layer liquid inlet hole and the liquid transfer cavity;
one end of the liquid drainage flow channel is respectively communicated with the liquid transfer layer reaction cavity and the liquid transfer cavity, and the other end of the liquid drainage flow channel is communicated with a liquid drainage hole of the thin film layer;
the pipetting layer reaction cavity is communicated with the pipetting cavity through a transfer flow channel;
one end of the pneumatic control layer air inlet flow channel is connected with a pneumatic control cavity, and the other end of the pneumatic control layer air inlet flow channel is connected with an external air source through a thin film layer air inlet hole, a pipetting layer air inlet hole, a reaction cavity layer air inlet hole, a transition layer air inlet hole and an air inlet hole;
and the liquid discharge hole of the pneumatic control layer is respectively communicated with the liquid transfer layer reaction containing cavity and the liquid transfer containing cavity through the liquid discharge hole of the thin film layer.
In the invention, the liquid inlet layer, the transition layer, the reaction cavity layer, the liquid transfer layer and the thin film layer are sealed to form a first cavity, and the reaction cavity layer, the liquid transfer layer and the thin film layer are sealed to form a second cavity;
in the invention, the pneumatic control cavity, the film layer, the liquid discharge channel and the transfer channel form a pneumatic micro-valve structure of a liquid channel-film-pneumatic control cavity.
In the invention, the number of the transition layer air inlet holes, the reaction cavity layer air inlet holes, the liquid transfer layer air inlet holes, the film layer air inlet holes and the pneumatic control layer air inlet channels is equal to that of the liquid inlet air inlet holes.
In the invention, the number of the liquid inlet air inlet channels is equal to that of the liquid inlet air holes.
In the invention, the number of the liquid discharge holes of the pneumatic control layer and the number of the liquid discharge holes of the thin film layer are equal to the number of the liquid discharge flow channels.
In the invention, the liquid inlet layer, the transition layer, the reaction cavity layer, the liquid transfer layer and the pneumatic control layer are made of PDMS, PMMA, glass or organic plastics and the like, and the thin film layer is made of PDMS.
In the invention, at least one of the liquid inlet flow channels is an inlet flow channel, and the rest are liquid inlet flow channels.
In the invention, the transition layer plays a role in realizing reliable liquid inlet and liquid discharge of the chip.
In the invention, the chip is horizontally arranged when in work.
Compared with the prior art, the invention has the following advantages:
1. the chip body is manufactured by adopting a soft etching packaging process, the surface characteristics of the liquid cavity and the flow channel are hydrophobic, no residual liquid is left after liquid is transferred or drained, no reagent cross contamination is caused when the liquid inlet and drainage steps are repeatedly carried out in the liquid cavity, and the quantity of the liquid cavity is reduced by recycling, so that the volume of the chip is reduced, and the integration of the microfluidic chip is facilitated.
2. The invention manufactures 2 liquid cavities with greatly different volumes on one chip through the combination of multilayer structures, and the liquid cavities are respectively used for finishing the sample processing processes of a large amount of reagents and a small amount of reagents, and the processing processes of different dosages are finished on the same chip, thereby greatly simplifying the operation.
3. The invention realizes the liquid inlet, liquid discharge, liquid transfer and the on-off control of the corresponding liquid flow channel by pneumatic control, thereby realizing the automation and integration of the reagent conveying and sample extracting processes in the microfluidic chip.
4. The invention realizes reliable work of liquid inlet and liquid discharge through the transition cavity.
Drawings
FIG. 1 is a schematic diagram of the entire structure of a microfluidic chip in example 1;
FIG. 2 is an exploded view of the structure of the microfluidic chip in example 1;
FIG. 3 is a schematic structural view of a feed liquid intake layer in example 1;
FIG. 4 is a schematic structural view of a transition layer in example 1;
FIG. 5 is a schematic view showing the structure of a cavity layer in example 1;
FIG. 6 is a schematic view showing the structure of a liquid-repellent layer in example 1;
FIG. 7 is a schematic view showing the structure of a thin film layer in example 1;
FIG. 8 is a schematic view showing the structure of a pneumatic control layer in example 1;
FIG. 9 is a bottom view of the feed liquid intake layer of example 1;
FIG. 10 is a bottom view of the liquid-transfer layer in example 1;
FIG. 11 is a view showing the entire structure of a microfluidic chip in example 2;
FIG. 12 is an exploded view of the structure of the microfluidic chip in example 2;
FIG. 13 is a schematic view showing the structure of a feed liquid intake layer in example 2;
FIG. 14 is a schematic structural view of a transition layer in example 2;
FIG. 15 is a schematic view showing the structure of a cavity layer in example 2;
FIG. 16 is a schematic view showing the structure of a liquid-repellent layer in example 2;
FIG. 17 is a schematic view showing the structure of a thin film layer in example 2;
FIG. 18 is a schematic view showing the structure of a pneumatic control layer in example 2;
FIG. 19 is a bottom view of the feed liquid intake layer of example 2;
fig. 20 is a bottom view of the liquid-transfer layer in example 2.
Detailed Description
The technical solutions of the present invention are further described below with reference to the following examples, but the present invention is not limited thereto, and any modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Example 1:
as shown in fig. 1 and fig. 2, the pneumatic horizontal microfluidic biomedical chip with a transition cavity in this embodiment is made of PDMS, and includes, from top to bottom, a liquid inlet layer 1, a transition layer 2, a reaction cavity layer 3, a liquid transfer layer 4, a thin film layer 5, and a pneumatic control layer 6.
As shown in fig. 3 and 9, the liquid inlet layer 1 has 4 liquid inlet holes 3101 and 4 gas inlet holes 3102, the 4 liquid inlet holes 3101 and the 4 gas inlet holes 3102 are uniformly distributed in two rows on the same side of the chip, the outer row is the gas inlet holes 3102, the inner row is the liquid inlet holes 3101, and the lower surface layer of the liquid inlet layer 1 has 4 liquid inlet channels 4101.
As shown in fig. 4, the transition layer 2 is distributed with a transition cavity 3201, a transition layer liquid inlet port 3202 and 4 transition layer inlet ports 3203.
As shown in fig. 5, reaction cavity layer 3 is distributed with reaction cavity 3301, reaction cavity layer liquid inlet 3302 and 4 reaction cavity layer inlet 3303.
The tail ends of the 4 liquid inlet channels 4101 are communicated with 4 liquid inlet holes 3101, wherein 2 liquid inlet channels 4101 are converged in a transition cavity 3201 and communicated with the reaction cavity 3301, and the other 2 liquid inlet channels 4101 are converged in a transition layer liquid inlet hole 3202.
As shown in fig. 6 and 10, a pipetting layer reaction cavity 3401, a pipetting cavity 3402, 4 pipetting layer air inlets 3403 are distributed on the pipetting layer 4, and a first drainage channel 4201, a second drainage channel 4203 and a transfer channel 4202 are distributed on the sealing side with the thin film layer 5, wherein: first drainage channel 4201 is communicated with pipetting layer reaction chamber 3401, second drainage channel 4203 is communicated with pipetting chamber 3402, pipetting layer reaction chamber 3401 is communicated with pipetting chamber 3402 through transfer channel 4202, and transition layer liquid inlet port 3202 is communicated with pipetting chamber 3402 through reaction chamber layer liquid inlet port 3302.
As shown in fig. 7, the film layer 5 is distributed with 2 film layer liquid discharge holes 3501 and 4 film layer air intake holes 3502, wherein: 2 film layer drainage apertures 3501 communicate with the ends of first and second drainage channels 4201 and 4203, respectively.
As shown in fig. 8, 2 pneumatic control layer liquid discharge holes 3601 are distributed on the pneumatic control layer 6, 4 pneumatic control layer air inlet channels 3602 and a first pneumatic control cavity 3603, a second pneumatic control cavity 3604, a third pneumatic control cavity 3605 and a fourth pneumatic control cavity 3606 which are located at the tail end of the pneumatic control layer air inlet channels 3602 are distributed on the sealing side of the thin film layer 5, wherein: the head ends of the 4 pneumatic control layer air inlet flow channels 3602 are connected with an external air source through a thin-film layer air inlet hole 3502, a liquid-transfer layer air inlet hole 3403, a reaction cavity layer air inlet hole 3303, a transition layer air inlet hole 3203 and an air inlet hole 3102, the pneumatic control layer liquid outlet hole 3601 is communicated with the thin-film layer liquid outlet hole 3501, and the first pneumatic control cavity 3603, the second pneumatic control cavity 3604, the third pneumatic control cavity 3605 and the fourth pneumatic control cavity 3606 are rectangular cavities.
The liquid inlet layer 1, the transition layer 2, the reaction chamber layer 3, the liquid transfer layer 4 and the thin film layer 5 are sealed to form a first chamber 11, and the reaction chamber layer 3, the liquid transfer layer 4 and the thin film layer 5 are sealed to form a second chamber 12.
The first pneumatic control chamber 3603, the second pneumatic control chamber 3604, the third pneumatic control chamber 3605, and the fourth pneumatic control chamber 3606 form a pneumatic micro valve structure of liquid flow channel-film-pneumatic control chamber with the film layer 5, the first drain flow channel 4201, the transfer flow channel 4202, and the second drain flow channel 4203.
The working principle of the pneumatic horizontal microfluidic biomedical chip with the transition cavity in the embodiment is as follows:
firstly, feed liquid
When the first cavity 11 is filled with liquid, gas is introduced from the gas inlet hole 3102, so that a certain gas pressure exists in the first pneumatic control cavity 3603 and the second pneumatic control cavity 3604, the pressure deforms the film layer contacted with the pneumatic control cavities, so that the first liquid discharge channel 4201 and the transfer channel 4202 are closed, solution is introduced from the liquid inlet hole 3101, and the solution enters the first cavity 11 through the transition cavity 3201 after entering the liquid inlet channel 4101; when the second cavity 12 is fed with liquid, gas is introduced through the gas inlet hole 3102, so that a certain gas pressure exists in the third pneumatic control cavity 3605, the pressure deforms the thin film layer in contact with the pneumatic control cavity, the transfer flow channel 4202 is closed, the solution is introduced through the liquid inlet hole 3101, and the solution enters the second cavity 12 through the transition layer liquid inlet hole 3202 and the reaction cavity layer liquid inlet hole 3302 after entering the liquid inlet flow channel 4101.
Second, transfer
After the liquid inlet process is completed, the gas source corresponding to the gas inlet hole 3102 is removed, the gas pressure in the second pneumatic control chamber 3604 is recovered, the transfer flow channel 4202 is opened, gas is introduced through the liquid inlet hole 3101, the solution in the first cavity 11 flows into the second cavity 12 through the transfer flow channel 4202 under the driving of the gas pressure, and the excess solution flows through the second liquid discharge flow channel 4203 and is discharged out of the chip from the liquid discharge port 3501.
Third, liquid discharge
When the first cavity is drained, the gas source corresponding to the gas inlet 3102 is removed, so that the gas pressure in the second pneumatic control cavity 3604 is maintained, the gas pressure in the first pneumatic control cavity 3603 is recovered, the first drain channel 4201 is opened, gas is introduced from the liquid inlet 3101, and the solution in the first cavity 11 is driven by the gas pressure to be drained out of the chip from the liquid outlet 3501 through the first drain channel 4201.
When the second cavity is drained, the gas source corresponding to the gas inlet 3102 is removed, so that the gas pressure in the third pneumatic control cavity 3603 is maintained, the gas pressure in the fourth pneumatic control cavity 3606 is recovered, the second liquid drainage flow channel 4203 is opened, gas is introduced from the liquid inlet 3101, and the solution in the second cavity 12 is driven by the gas pressure to be drained out of the chip from the liquid drainage port 3501 through the second liquid drainage flow channel 4203.
Example 2:
as shown in fig. 11 and 12, the pneumatic horizontal microfluidic biomedical chip with a transition cavity in this embodiment is made of PDMS, and includes, from top to bottom, a liquid inlet layer 1, a transition layer 2, a reaction cavity layer 3, a liquid transfer layer 4, a thin film layer 5, and a pneumatic control layer 6.
As shown in fig. 13 and 19, the feed liquid air intake layer 1 has 4 feed liquid air intake holes 5101 and 4 air intake holes 5102, the 4 feed liquid air intake holes 5101 and 4 air intake holes 5102 are distributed on two sides of the chip, and the lower surface layer of the feed liquid air intake layer 1 has 4 feed liquid air intake channels 6101.
As shown in fig. 14, the transition layer 2 is distributed with a transition cavity 5201, a transition layer liquid inlet hole 5202 and 4 transition layer inlet holes 5203.
As shown in fig. 15, reaction cavity layer 3 is distributed with reaction cavity 5301, reaction cavity layer liquid inlet hole 5302 and 4 reaction cavity layer inlet holes 5303.
The tail ends of the 4 liquid inlet channels 6101 are communicated with 4 liquid inlet holes 5101, wherein 3 liquid inlet channels 6101 are converged in the transition cavity 5201 and communicated with the reaction cavity 5301, and the other 1 gas inlet channel 6101 is converged in the transition layer liquid inlet hole 5202.
As shown in fig. 16 and fig. 20, the liquid transfer layer 4 is distributed with a liquid transfer layer reaction cavity 5401, a liquid transfer cavity 5402, 4 liquid transfer layer air inlets 5403, and a first liquid discharge channel 6201, a second liquid discharge channel 6203, a third liquid discharge channel 6204 and a transfer channel 6202 on the sealing side with the thin film layer 5, wherein: the first liquid discharge flow passage 6201 is communicated with the pipetting layer reaction cavity 5401, the second liquid discharge flow passage 6203 and the third liquid discharge flow passage 6204 are respectively communicated with the pipetting cavity 5402, the pipetting layer reaction cavity 5401 and the pipetting cavity 5402 are communicated through the transfer flow passage 6202, and the transition layer liquid inlet hole 5202 is communicated with the pipetting cavity 5402 through a reaction cavity layer liquid inlet hole 5302.
As shown in fig. 17, the film layer 5 is distributed with 3 film layer liquid discharge holes 5501 and 4 film layer air inlet holes 5502, wherein: 3 film layer drain holes 5501 are connected to the ends of first drain flow channel 6201, second drain flow channel 6203, and third drain flow channel 6204, respectively.
As shown in fig. 18, 3 pneumatic control layer drain holes 5601 are distributed on the pneumatic control layer 6, 4 pneumatic control layer inlet flow channels 5602 and a first pneumatic control cavity 5603, a second pneumatic control cavity 5604, a third pneumatic control cavity 5605, a fourth pneumatic control cavity 5606 and a fifth pneumatic control cavity 5607 located at the end of the pneumatic control layer inlet flow channel 5602 are distributed on the sealing side of the thin film layer 5, wherein: the head ends of the 4 pneumatic control layer air inlet flow channels 5602 are connected with an external air source through a thin film layer air inlet hole 5502, a pipetting layer air inlet hole 5403, a reaction cavity layer air inlet hole 5303, a transition layer air inlet hole 5203 and an air inlet hole 5102, a pneumatic control layer liquid outlet hole 5601 is communicated with the thin film layer liquid outlet hole 5501, and the first pneumatic control cavity 5603, the second pneumatic control cavity 5604, the third pneumatic control cavity 5605, the fourth pneumatic control cavity 5606 and the fifth pneumatic control cavity 5607 are rectangular cavity.
The liquid inlet layer 1, the transition layer 2, the reaction chamber layer 3, the liquid transfer layer 4 and the thin film layer 5 are sealed to form a first chamber 21, and the reaction chamber layer 3, the liquid transfer layer 4 and the thin film layer 5 are sealed to form a second chamber 22.
The first pneumatic control chamber 5603, the second pneumatic control chamber 5604, the third pneumatic control chamber 5605, the fourth pneumatic control chamber 5606, the fifth pneumatic control chamber 5607, the membrane layer 5, the first drain flow passage 6201, the transfer flow passage 6202, the second drain flow passage 6203, and the third drain flow passage 6204 form a pneumatic microvalve structure of a liquid flow passage-membrane-pneumatic control chamber.
The working principle of the pneumatic horizontal microfluidic biomedical chip with the transition cavity in the embodiment is as follows:
firstly, feed liquid
When the first cavity 21 is fed with liquid, gas is introduced from the gas inlet hole 5102, so that certain gas pressure exists in the first pneumatic control cavity 5603, the second pneumatic control cavity 5604 and the third pneumatic control cavity 5605, the pressure deforms the film layer in contact with the pneumatic control cavities, so that the first exhaust flow passage 6201 and the transfer flow passage 6202 are closed, the solution is introduced from the liquid inlet hole 5101, and the solution enters the first cavity 21 through the transition cavity 5201 after entering the liquid inlet flow passage 6101; when the second cavity 22 is fed with liquid, gas is introduced through the gas inlet hole 5102, so that a certain gas pressure exists in the third pneumatic control cavity 5605 and the fourth pneumatic control cavity 5606 or the fifth pneumatic control cavity 5607, the pressure deforms the thin film layer in contact with the pneumatic control cavity, the transfer flow passage 6202 and the second liquid discharge flow passage 6203 or the third liquid discharge flow passage 6204 are closed, the solution is introduced through the liquid inlet hole 5101, and the solution enters the liquid inlet flow passage 6101, passes through the transition layer liquid inlet hole 5202 and the reaction cavity layer liquid inlet hole 5302, and enters the second cavity 22.
Second, transfer
After the liquid inlet process of the first cavity 21 is completed, the gas source corresponding to the gas inlet 5102 is removed, so that the air pressure in the third pneumatic control cavity 5605 is recovered, the transfer flow passage 6202 is opened, gas is introduced from the liquid inlet 5101, the solution in the first cavity 21 flows into the second cavity 22 through the transfer flow passage 6202 under the driving of the air pressure, and the excessive solution flows through the third liquid discharge flow passage 6203 or the fourth liquid discharge flow passage 6204 and is discharged out of the chip from the liquid discharge port 5501.
Third, liquid discharge
When the first cavity discharges liquid, the corresponding gas source in the gas inlet hole 5102 is removed, so that the gas pressure in the third pneumatic control cavity 5605 is maintained, the gas pressures in the first pneumatic control cavity 5603 and the second pneumatic control cavity 5604 are recovered, the first liquid discharge channel 6201 is opened, gas is introduced from the liquid inlet hole 5101, and the solution in the first cavity 21 is driven by the gas pressure to be discharged out of the chip from the liquid discharge port 5501 through the first liquid discharge channel 6201.
When the second cavity drains, the air source corresponding to the air inlet 5102 is removed, so that the air pressure in the third pneumatic control cavity 5605 is maintained, the air pressures in the fourth pneumatic control cavity 5606 and the fifth pneumatic control cavity 5607 are recovered, the second liquid drainage flow channel 6203 or the third liquid drainage flow channel 6204 is opened, air is introduced from the liquid inlet 5101, and the solution in the second cavity 22 flows through the second liquid drainage flow channel 6203 or the third liquid drainage flow channel 6204 under the driving of the air pressure, and is discharged out of the chip from the liquid drainage port 5501.
Claims (9)
1. A pneumatic horizontal microfluidic biomedical chip with a transition cavity is characterized in that the chip is of a multilayer structure and comprises a liquid inlet layer, a transition layer, a reaction cavity layer, a liquid transfer layer, a thin film layer and a pneumatic control layer;
the liquid inlet layer is distributed with a liquid inlet hole and an air inlet hole, and one side of the liquid inlet layer sealed with the transition layer is distributed with a liquid inlet flow channel;
the transition layer is provided with a transition cavity, a transition layer liquid inlet hole and a transition layer air inlet hole;
the reaction cavity layer is provided with a reaction cavity, a reaction cavity layer liquid inlet hole and a reaction cavity layer air inlet hole;
the liquid transfer layer is provided with a liquid transfer layer reaction cavity, a liquid transfer cavity and a liquid transfer layer air inlet, and one side of the liquid transfer layer sealed with the thin film layer is provided with a liquid discharge flow channel and a transfer flow channel;
the film layer is distributed with film layer liquid discharge holes and film layer air inlet holes;
the pneumatic control layer is distributed with pneumatic control layer liquid discharge holes, and one side of the pneumatic control layer sealed with the thin film layer is distributed with a pneumatic control layer air inlet channel and a pneumatic control cavity;
the liquid inlet holes and the air inlet holes are distributed on one side or two sides of the liquid inlet layer;
at least one of the liquid inlet flow channels is an inlet flow channel, and the rest are liquid inlet flow channels;
one end of the air inlet flow channel is communicated with the liquid inlet hole, and the other end of the air inlet flow channel is communicated with the liquid inlet hole of the transition layer;
one end of the liquid inlet flow channel is communicated with a liquid inlet hole, and the other end of the liquid inlet flow channel is communicated with the transition cavity;
the transition cavity is communicated with the reaction cavity and the pipetting layer reaction cavity;
the transition layer liquid inlet hole is communicated with the reaction cavity layer liquid inlet hole and the liquid transfer cavity;
one end of the liquid drainage flow channel is respectively communicated with the liquid transfer layer reaction cavity and the liquid transfer cavity, and the other end of the liquid drainage flow channel is communicated with a liquid drainage hole of the thin film layer;
the pipetting layer reaction cavity is communicated with the pipetting cavity through a transfer flow channel;
one end of the pneumatic control layer air inlet flow channel is connected with a pneumatic control cavity, and the other end of the pneumatic control layer air inlet flow channel is connected with an external air source through a thin film layer air inlet hole, a pipetting layer air inlet hole, a reaction cavity layer air inlet hole, a transition layer air inlet hole and an air inlet hole;
and the liquid discharge hole of the pneumatic control layer is respectively communicated with the liquid transfer layer reaction containing cavity and the liquid transfer containing cavity through the liquid discharge hole of the thin film layer.
2. The pneumatic horizontal microfluidic biomedical chip with transition volume according to claim 1, wherein the liquid inlet layer, the transition layer, the reaction volume layer, the liquid transfer layer and the thin film layer enclose a first volume, and the reaction volume layer, the liquid transfer layer and the thin film layer enclose a second volume.
3. The pneumatic horizontal microfluidic biomedical chip with transshipment cavity of claim 1, wherein the pneumatic control cavity, the thin film layer, the liquid drainage channel and the transfer channel form a pneumatic micro valve structure of liquid channel-thin film-pneumatic control cavity.
4. The pneumatic horizontal microfluidic biomedical chip with a transition cavity of claim 1, wherein the number of the transition layer air inlet holes, the reaction cavity layer air inlet holes, the pipetting layer air inlet holes, the thin film layer air inlet holes and the pneumatic control layer air inlet channels is equal to the number of the inlet holes of the inlet liquid layer.
5. The pneumatic horizontal microfluidic biomedical chip with a transition volume according to claim 1, wherein the number of inlet liquid flow channels is equal to the number of inlet liquid inlet holes.
6. The pneumatic horizontal microfluidic biomedical chip with a transition volume according to claim 1, wherein the number of drainage holes of the pneumatic control layer and the number of drainage holes of the thin film layer are equal to the number of drainage channels.
7. The pneumatic horizontal microfluidic biomedical chip with a transition volume chamber according to claim 1, wherein the materials of the liquid inlet layer, the transition layer, the reaction volume chamber layer, the liquid transfer layer and the pneumatic control layer are PDMS, PMMA, glass or organic plastics.
8. The pneumatic horizontal microfluidic biomedical chip with a transition volume according to claim 1, wherein the material of the thin film layer is PDMS material.
9. The pneumatic horizontal microfluidic biomedical chip with transreception cavity according to claim 1, wherein said chip is horizontally disposed during operation.
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| CN106065391A (en) * | 2016-07-20 | 2016-11-02 | 国家纳米科学中心 | For unicellular sorting and the micro-fluidic chip of unicellular whole genome amplification |
| CN106955750A (en) * | 2017-04-01 | 2017-07-18 | 苏州岚轩生物科技有限公司 | The micro-fluidic chip flowed based on active control liquid |
| CN206951224U (en) * | 2017-06-15 | 2018-02-02 | 南京巴傲得生物科技有限公司 | A kind of micro-fluid chip in the experiment for WB |
| CN107511188A (en) * | 2017-07-03 | 2017-12-26 | 昆明理工大学 | One kind is based on chip lab micro-fluidic technologies gas control microvalve device and its control method |
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