CN206887110U - A kind of PCR pipe, PCR pipe bar - Google Patents

Abstract

The utility model discloses a kind of PCR pipe, including the body and lid mutually covered, the lid includes stretching into the connecting portion of tubular body and the cap portion outside body when covering, the body and lid are separate, and the inwall of body and the connecting portion of lid are respectively equipped with the internal thread and external screw thread being engaged.The utility model PCR pipe is by the way that body and lid are designed independently of each other, the inwall of body and the connecting portion of lid are respectively equipped with the internal thread and external screw thread being engaged, during use, the connecting portion of lid is screwed in body, phenomena such as being coordinated by screw thread strengthens the sealing intensity of lid and body, avoids that expansion occurs after amplified reaction terminates, and lid relaxes, solves Aerosol, so as to avoid the appearance of false positive.

Description

A kind of PCR pipe, PCR pipe bar

Technical field

Biotechnology consumptive material field is the utility model is related to, more particularly to a kind of PCR pipe, PCR pipe bar.

Background technology

Polymerase chain amplification reaction (PCR) technology of Kary Mullis inventions is molecular biology the 1980s One of field is revolutionary to be broken through.With the development of science and technology, various nucleic acid detection techniques are in molecular biology, medical science, method Etc. the application field such as basic research and medical diagnosis, food safety detection plays extremely important effect.At present, commonly use Nucleic acid amplification method have based on classical PCR and the emerging major class of isothermal amplification technique two, such as cross primer constant-temperature amplification (CPA), loop-mediated isothermal amplification (LAMP), strand displacement amplification (SDA), the amplification (HDA) for relying on unwindase, restructuring polymerase expand Increase (RPA), the amplification (RAA) of recombinase-mediated, rolling circle amplification (RCA) and rapid isothermal detection and amplify technologies such as (RIDA).By It is very high in the high sensitivity of nucleic acid amplification technologies, the yield of amplified matter.

The body and lid of conventional PCR pipe interconnect, and lid inserts body by pressing the portion of connecting It is interior, then keep sealing by the annular protrusion on connecting portion and inboard wall of tube body.For example Authorization Notice No. is CN203307336U Chinese utility model patent disclose a kind of PCR pipe, including at least one body and the lid that is engaged with the body are described Body be made up of PP materials, the main body of body including tubulose and the conical bottom being connected with main body, lid are made up of PP materials, The auxiliary section of body and lid is provided with sealing shroud annular made of TPU materials, and sealing shroud is fixed with body.

Although such structure design makes PCR pipe more convenient when using, expansion occurs after amplified reaction terminates, Phenomena such as lid relaxes, the Aerosol at laboratory or scene is easily caused, so that false sun occurs in testing result The problems such as property.This limits nucleic acid amplification technologies and broadly can not promote and apply to a certain extent, particularly with field Or the detection of nucleic acids at scene.Therefore, a kind of anti-expansion of exploitation, the PCR pipe of tight hermetic are extremely urgent.

Utility model content

The utility model occurs that expansion, lid relaxation etc. are asked for PCR pipe of the prior art after PCR amplifications terminate Topic, there is provided a kind of PCR pipe of good airproof performance, PCR pipe bar.

A kind of PCR pipe, including the body and lid mutually covered, the lid include stretching into the company of tubular body when covering Socket part and the cap portion outside body, the body and lid are separate, the inwall of body and the coupling part of lid The internal thread and external screw thread that She You be engaged.

Preferably, the internal thread of the inboard wall of tube body is 3~5 circles.

Preferably, the connecting portion is provided with first annular groove, first ring in the front end of external screw thread region Sealing ring is provided with connected in star, the inwall of the body is provided with the second annular groove being engaged with sealing ring.

It is further preferred that nearby tube wall thickeies the oral area of the body, the internal thread and the second annular groove are all provided with It is placed in the part of the thickening.

Preferably, the side wall of the cap portion is provided with anti-slip veins.

Preferably, the material of the PCR pipe is polypropylene.

A kind of PCR pipe bar, it is formed by connecting by multiple described PCR pipe word orders, passes through position between two neighboring body It is connected in the connection strap of body oral area.

Preferably, the quantity of the PCR pipe is 8.

The utility model PCR pipe is by the way that body and lid are designed independently of each other, the inwall of body and the connecting portion of lid The internal thread and external screw thread being engaged are respectively equipped with, in use, the connecting portion of lid is screwed in body, coordinating by screw thread strengthens The sealing intensity of lid and body, avoid that expansion occurs after amplified reaction terminates, lid relax phenomena such as, solve aerosol Pollution problem, so as to avoid the appearance of false positive.

Brief description of the drawings

Fig. 1 is the structural representation of the utility model PCR pipe.

Fig. 2 is the structural representation of the utility model body.

Fig. 3 is the structural representation of the lid with sealing ring.

Fig. 4 is the structural representation without the lid of sealing ring.

Fig. 5 is the structural representation of the PCR pipe bar of non-cap body.

Embodiment

As shown in figures 1-4, a kind of PCR pipe, including the body 1 and lid 2 mutually covered, body 1 and lid 2 are mutually only It is vertical.Body 1 includes the conical section 11 of bottom and the vertical section 12 on top.Lid 2 includes the connection stretched into when covering inside body 1 Portion 21 and the cap portion 22 outside body 1.The material of PCR pipe is polypropylene.

The inwall of body 1 and the connecting portion 21 of lid 2 are respectively equipped with the internal thread and external screw thread being engaged.The inwall of body 1 Internal thread for 3~5 circle.Thread turns is very few, and the sealing intensity of body 1 and lid 2 is weaker, and thread turns is excessive, then covers Close and opening operation is relatively complicated.

The front end that the connecting portion 21 of lid 2 is located at external screw thread region is provided with first annular groove 23, first annular recessed Sealing ring 3 is provided with groove 23, the inwall of body 1 is provided with the second annular groove 14 being engaged with sealing ring 3.One side lid 2 Coordinate the bond strength and tightness that enhance between the two with the screw thread of body 1, while the setting of sealing ring 3 further enhances Sealing effectiveness.

In order to avoid body 1 and lid 2 cover region partially it is thin cause PCR react after the place of covering easily deform, will manage The top of the vertical section 12 of body 1, which thickeies, turns into thick section 13, and internal thread and the second annular groove 14 are respectively positioned on thick section 13 The inside divided.

Because PCR pipe is smaller, regular size is 200 μ L, in order to prevent that lid 2 is affectedly bashful inconvenience when operating, it is possible to will The cap portion 22 of lid 2 does thickening processing, certainly, now needs suitably to reduce the height of body 1, to adapt to PCR instrument device.When So, in order to anti-skidding, anti-slip veins can be provided with the side wall of the cap portion 22 of lid 2.

In use, toward being loaded in body 1, the connecting portion 21 of body 2 is screwed in body 1 after adding sample, makes both screw threads Coordinate, while be arranged in the sealing ring 3 on connecting portion 21 in first annular groove 23 and be caught in the second annular groove 14.PCR is anti- Should after the completion of, then lid 2 screwed out into opening.

As shown in figure 5, multiple PCR pipe connections of the present utility model can be made into PCR pipe bar, typically conventional connects for 8 8 PCR pipe word orders, i.e., be formed by connecting, pass through the phase of connection strap 4 positioned at the oral area of body 1 between two neighboring PCR pipe by pipe Even, body 1 is only depicted in Fig. 5 and is not drawn into lid 2, needs during use to screw in lid 2 into body 1 one by one and seals.

Claims (8)

1. a kind of PCR pipe, including the body and lid mutually covered, the lid includes stretching into the connection of tubular body when covering Portion and the cap portion outside body, it is characterised in that the body and lid are separate, the inwall of body and lid Connecting portion is respectively equipped with the internal thread and external screw thread being engaged.

2. PCR pipe as claimed in claim 1, it is characterised in that the internal thread of the inboard wall of tube body is 3~5 circles.

3. PCR pipe as claimed in claim 1, it is characterised in that the connecting portion is set in the front end of external screw thread region There is first annular groove, sealing ring is provided with the first annular groove, the inwall of the body is provided with to be engaged with sealing ring The second annular groove.

4. PCR pipe as claimed in claim 3, it is characterised in that nearby tube wall thickeies the oral area of the body, the internal thread The part of the thickening is may be contained within the second annular groove.

5. PCR pipe as claimed in claim 1, it is characterised in that the side wall of the cap portion is provided with anti-slip veins.

6. the PCR pipe as described in Claims 1 to 5 is any, it is characterised in that the material of the PCR pipe is polypropylene.

7. a kind of PCR pipe bar, it is characterised in that connected by multiple PCR pipe word orders as described in Claims 1 to 5 is any Form, by being connected positioned at the connection strap of body oral area between two neighboring body.

8. PCR pipe bar as claimed in claim 7, it is characterised in that the quantity of the PCR pipe is 8.