CN106047660A - Nucleic acid extraction device for nucleic acid isolation machine - Google Patents
Nucleic acid extraction device for nucleic acid isolation machine Download PDFInfo
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- CN106047660A CN106047660A CN201610527509.0A CN201610527509A CN106047660A CN 106047660 A CN106047660 A CN 106047660A CN 201610527509 A CN201610527509 A CN 201610527509A CN 106047660 A CN106047660 A CN 106047660A
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- nucleic acid
- syringe
- tubular body
- aspirator
- extraction device
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Bioinformatics & Cheminformatics (AREA)
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Abstract
The invention discloses a nucleic acid extraction device for a nucleic acid isolation machine. The nucleic acid extraction device comprises a tubular body and an aspirator. The top opening of the tubular body is provided with a connector. The aspirator is connected with the tubular body through the connector. The bottom of the tubular body is provided with a plug. A filter element device is arranged in the plug. The nucleic acid extraction device has the advantages that the nucleic acid extraction device replaces a high-rotation-speed centrifuge, can achieve a good separation effect in a liquid-phase heterogeneous system and is low in cost, simple to operate, short in operation time and widely applicable to nucleic acid rapid diagnosis of primary hospitals and emergency rooms with relatively poor experiment conditions. The aspirator is specifically a syringe. The syringe comprises a syringe body, a connecting rod and a sealing part, wherein the sealing part is arranged at the bottom of the connecting rod, a needle part is arranged at the bottom of the syringe body, a protruding head matched with the needle part is arranged at the bottom of the sealing part, the sealing part is pushed into the bottommost part of the syringe body before use to allow the protruding head to fill the needle part so as to avoid residual air in the needle part, and influence of the residual air to the extraction can be avoided.
Description
Technical field
The present invention relates to technical field of medical equipment, particularly relate to a kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus.
Background technology
The biomacromolecule compound that nucleic acid is become by many nucleotide polymerization, for one of the most basic material of life.Nucleic acid
Being widely present in all animal and plant cellss, microbial body, the nucleic acid in organism is often and protein bound forms nucleoprotein.No
Same nucleic acid, its difference such as chemical composition, nucleotidesequence.
Nanometer magnetic bead is a kind of surface super-paramagnetism nano microsphere with carboxyl, is stably dispersed in water and PH is more than
In the biological buffer solutions of 6.5.Its rich surface contains carboxyl, and this carboxyl can have super because of it with biological aglucon covalent bond simultaneously
Paramagnetism, can realize sharp separation under externally-applied magnetic field, therefore can be widely used in biomarker, immunologic diagnosis, protein divide
From fields such as purification, cell separation, immobilized enzyme, biosensors, it is particularly suited for high throughput automated platform.
Almost at each laboratory, the isolated and purified work relevant to biomolecule is all particularly significant and essential
, it is therefore desirable to a simple and practical nucleic acid-extracting apparatus.
Summary of the invention
The invention aims to solve shortcoming present in prior art, and a kind of instrument for extracting nucleic acid core proposed
Acid extraction element.
To achieve these goals, present invention employs following technical scheme:
A kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus, including tubular body and aspirator, the open top of described tubular body
Place is provided with joint, and aspirator is connected with tubular body by joint, and the bottom of tubular body is provided with plug, is provided with in described plug
Filter element device；
Described aspirator is specially syringe, and including syringe, connecting rod, handle and sealing, described connecting rod is by the top of syringe
Portion's opening stretches in syringe, and the top of connecting rod is provided with handle, and the bottom of connecting rod is provided with sealing；The bottom of described syringe sets
Having needle section, the bottom of sealing is provided with the plush copper matched with needle section, and when sealing is positioned at bottom syringe, plush copper is full of pin
Head.
Preferably, described plug is taper.
Preferably, described filter element device includes primary filter element layer and is located at primary secondary filter element layer within filter element layer.
Compared with prior art, the invention has the beneficial effects as follows:
1, the nucleic acid-extracting apparatus of the present invention instead of high speed centrifuge, can reach good dividing in liquid phase heterogeneous system
From effect and with low cost, simple to operate, the operating time is short, can be widely applied to the basic unit doctor that experiment condition is relatively poor
The nucleic acid quick diagnosis of institute and emergency room.
2, the bottom of sealing is provided with the plush copper matched with needle section, and when sealing is positioned at bottom syringe, plush copper is full of pin
Head, can avoid residual air in needle section, produces impact to extracting.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus that the present invention proposes.
In figure: 1, tubular body, 2, plug, 3, filter element device, 4, joint, 5, syringe, 6, needle section, 7, connecting rod, 8,
Handle, 9, sealing, 10, plush copper.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention rather than whole embodiments.
With reference to Fig. 1, a kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus, including tubular body 1 and aspirator, described tubulose master
The top open part of body 1 is provided with joint 4, and aspirator is connected with tubular body 1 by joint 4, and the bottom of tubular body 1 is provided with slotting
2, described plug 2 is taper.Being provided with filter element device 3 in described plug 2, described filter element device 3 includes primary filter element layer and sets
In the secondary filter element layer within primary filter element layer.The nucleic acid-extracting apparatus of the present invention instead of high speed centrifuge, can be in liquid phase
Heterogeneous system reaches good separating effect, and with low cost, and simple to operate, the operating time is short, can be widely applied to
Basic hospital that experiment condition is relatively poor and the nucleic acid quick diagnosis of emergency room.
Described aspirator is specially syringe, including syringe 5, connecting rod 7, handle 8 and sealing 9, described connecting rod 7 by
The open top of syringe 5 is stretched in syringe 5, and the top of connecting rod 7 is provided with handle 8, and the bottom of connecting rod 7 is provided with sealing 9.Institute
The bottom stating syringe 5 is provided with needle section 6, and the bottom of sealing 9 is provided with the plush copper 10 matched with needle section 6, and sealing 9 is positioned at
Time bottom syringe 5, plush copper 10 is full of needle section 6.Before using, sealing 9 is pushed syringe 5 bottommost, make plush copper 10 be full of syringe needle
Portion 6, then inserts needle section 6 joint 4 of tubular body 1, then aspirates, can avoid residual air in needle section 6, to carrying
Take generation impact.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (3)
1. an instrument for extracting nucleic acid nucleic acid-extracting apparatus, including tubular body (1) and aspirator, it is characterised in that described pipe
The top open part of shape main body (1) is provided with joint (4), and aspirator is connected with tubular body (1) by joint (4), tubular body
(1) bottom is provided with plug (2), is provided with filter element device (3) in described plug (2)；
Described aspirator is specially syringe, including syringe (5), connecting rod (7), handle (8) and sealing (9), described connection
Bar (7) is stretched in syringe (5) by the open top of syringe (5), and the top of connecting rod (7) is provided with handle (8), connecting rod (7)
Bottom is provided with sealing (9)；The bottom of described syringe (5) is provided with needle section (6), and the bottom of sealing (9) is provided with and needle section
(6) plush copper (10) matched, when sealing (9) is positioned at syringe (5) bottom, plush copper (10) is full of needle section (6).
A kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus the most according to claim 1, it is characterised in that described plug (2)
For taper.
A kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus the most according to claim 1, it is characterised in that described filter element device
(3) include primary filter element layer and be located at primary secondary filter element layer within filter element layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610527509.0A CN106047660A (en) | 2016-07-07 | 2016-07-07 | Nucleic acid extraction device for nucleic acid isolation machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610527509.0A CN106047660A (en) | 2016-07-07 | 2016-07-07 | Nucleic acid extraction device for nucleic acid isolation machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106047660A true CN106047660A (en) | 2016-10-26 |
Family
ID=57201090
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610527509.0A Pending CN106047660A (en) | 2016-07-07 | 2016-07-07 | Nucleic acid extraction device for nucleic acid isolation machine |
Country Status (1)
| Country | Link |
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| CN (1) | CN106047660A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019174468A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Positive pressure apparatus for nucleic acid extraction and purification |
| WO2019174480A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Nucleic acid extraction and filtration column |
| CN113249188A (en) * | 2021-06-03 | 2021-08-13 | 宁波康程德诺生物医药有限公司 | Rapid nucleic acid extraction device and method for extracting nucleic acid |
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| CN102430438A (en) * | 2010-08-30 | 2012-05-02 | 埃佩多夫股份公司 | Syringe for use with a metering device |
| CN202465673U (en) * | 2012-03-07 | 2012-10-03 | 天根生化科技（北京）有限公司 | Blood clot treatment column device for nucleic acid purification |
| CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
| CN204185484U (en) * | 2014-09-28 | 2015-03-04 | 南京钟鼎生物技术有限公司 | A kind of rapid extraction device for nucleic acid |
| CN205917253U (en) * | 2016-07-07 | 2017-02-01 | 张雯 | Nucleic acid extraction device for nucleic acid extractor |
-
2016
- 2016-07-07 CN CN201610527509.0A patent/CN106047660A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102430438A (en) * | 2010-08-30 | 2012-05-02 | 埃佩多夫股份公司 | Syringe for use with a metering device |
| CN202465673U (en) * | 2012-03-07 | 2012-10-03 | 天根生化科技（北京）有限公司 | Blood clot treatment column device for nucleic acid purification |
| CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
| CN204185484U (en) * | 2014-09-28 | 2015-03-04 | 南京钟鼎生物技术有限公司 | A kind of rapid extraction device for nucleic acid |
| CN205917253U (en) * | 2016-07-07 | 2017-02-01 | 张雯 | Nucleic acid extraction device for nucleic acid extractor |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019174468A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Positive pressure apparatus for nucleic acid extraction and purification |
| WO2019174480A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Nucleic acid extraction and filtration column |
| CN113249188A (en) * | 2021-06-03 | 2021-08-13 | 宁波康程德诺生物医药有限公司 | Rapid nucleic acid extraction device and method for extracting nucleic acid |
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Application publication date: 20161026 |