CN206740777U - A kind of detection card that can intercept MP non-specificity material and hemoglobin - Google Patents
A kind of detection card that can intercept MP non-specificity material and hemoglobin Download PDFInfo
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- CN206740777U CN206740777U CN201720563142.8U CN201720563142U CN206740777U CN 206740777 U CN206740777 U CN 206740777U CN 201720563142 U CN201720563142 U CN 201720563142U CN 206740777 U CN206740777 U CN 206740777U
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Abstract
The utility model provides a kind of detection card that can intercept MP non-specificity material and hemoglobin, the detection card includes getting stuck and reagent strip, it is described to get stuck including cap and shell bottom, the cap is covered on shell bottom top, shell bottom upper surface is provided with the neck for being used for placing reagent strip, interception line is provided with the reagent strip, the cap upper surface is provided with observation window and well, glass fibre element film is provided with the well.The beneficial effects of the utility model are the additions of interception line so that race film is more preferable, and background is cleaner, and technique is simple, reduces great amount of cost;Interception line is anti-human RBC antibody, and the anti-human RBC antibody that fixed amount is added in sample pad coordinates, and double intercepting systems of the anti-human RBC antibody in interception line can play a part of intercepting hemoglobin completely;Sample-adding hole site adds one layer of treated glass fibre element film, can intercept the nonspecific interference material run into MP products, reduce false positive rate, improve the degree of accuracy.
Description
Technical field
The utility model belongs to Eaton agent pneumonia MP detection fields, and MP non-specificity things can be intercepted more particularly, to one kind
The detection card of matter and hemoglobin.
Background technology
Eaton agent pneumonia (mycoplasma pneumonia, abbreviation MP) is also known as primary atypical pneumonia, condensation collection sun
Property pneumonia, be " SARS " in one kind, be as caused by mycoplasma infection, in interstitial pneumonia and bronchiolitis
Sample changes, and accounts for the 10%%~40% of community-acquired pneumonia in children, pulmonary lesion is in confluent bronchopneumonia, chromic fibrous lung
Inflammation, with bronchitis.Alveolar has a small amount of inflammatory exudate, and focal atelectasis, pulmonary consolidation and pulmonary emphysema can occur.Clinical manifestation
The lung inflammation acutely coughed for intractable.MP is one of Childhood pneumonia and the important pathogen of other respiratory tract infection.Lung
Scorching mycoplasma can cause tonsillitis, rhinitis, tympanitis, tracheitis, bronchiolitis, pneumonia;Child suffers from respiratory tract more
Infection;School age youngster suffers from an inflammation of the lungs more.
The method of usual pneumonia diagnosis has medical imaging, is separately cultured and Serologic detection.ELASA in serology
(ELISA) is common methods, and about 1-2 hours, collaurum product, small volume are easy to use often, it is not necessary to large-scale instrument
Device, try out in various occasions, and single detection 10-20 minutes.The expense of detection is saved, while shorter time goes out knot
Fruit, doctor can be coordinated to make diagnosis, make patient's obtaining medical treatment earlier, early rehabilitation.
In mycoplasma pneumoniae IgM antibody detection collaurum product, nonspecific interference material is had, shadow can be caused to experiment
Ring, make accuracy, false positive increases.In order to remove it, usual way is, into the formula of sample pad, it is appropriate to add
Albumen, for combine non-specific material, play a part of interception, still, experiment development, it is found that, be added to
The long period is needed to dry after in sample pad, it has not been convenient to quick detection, and utilization rate is low, and high-volume is prepared and small lot
Larger error occurs in preparation;Reincarnation postpartum, it is found that, the inventory of such a method consumption can be very big, once occur larger
Error, cost also can be in the growth of geometry times.In addition, also there is whole blood test test paper in collaurum in the market.This test strips need
A layers of blood separating film added at sample pad end, or add anti-human RBC in sample pad and play interception hemoglobin, make nitric acid fine
The background race sample for tieing up plain film is clean.Two kinds of products at the moment respectively have advantage and disadvantage, and anti-human RBC antibody is added into sample pad, though can
To intercept hemoglobin, still, addition needs to adjust repeatedly every time, moreover, there is small error when preparing, then run
During sample, background can be slightly red, and unnecessary trouble is caused to experimental result.Although and hemofiltration film can largely remove it is blood red
Albumen so that clean background, still, material are thicker, it is also necessary to connect each position with adhesive tape, play stable effect, more
It is cumbersome.
The content of the invention
In view of this, the utility model is directed to a kind of detection that can intercept MP non-specificity material and hemoglobin
Card, to solve above-mentioned technical problem.
To reach above-mentioned purpose, what the technical solution of the utility model was realized in:
A kind of detection card that can intercept MP non-specificity material and hemoglobin, the detection card includes getting stuck and reagent
Bar;
Described to get stuck including cap and shell bottom, the cap is covered on shell bottom top, and the upper surface of the cap, which is provided with, to be seen
Window and well are examined, glass fibre element film is provided with the well;The upper surface at the shell bottom, which is provided with, to be used to place reagent strip
Neck;
The reagent strip includes the big plates of PVC, and the big plate surfaces of PVC are provided with sticky paper, and the surface mount of the sticky paper has nitric acid fine
Plain film, gold pad, sample pad and absorbent filter are tieed up, the head end of the gold pad is overlapped in the head end of nitrocellulose filter, the sample
The head end of pad is overlapped in the tail end of gold pad, and the head end of the absorbent filter is overlapped in the tail end of nitrocellulose filter;The nitric acid
The surface of cellulose membrane is coated with interception line, detection line and nature controlling line successively along liquid chromatography(LC) direction, is provided with the sample pad
Anti-human RBC antibody.
Further, the interception line is anti-human RBC antibody.
Further, the interception line is apart from 3~4cm of absorbent filter tail end.
Further, the length that the gold pad is overlapped in nitrocellulose filter surface is 1-1.5mm.
Further, the nature controlling line is apart from 2~2.5cm of absorbent filter tail end, and the detection line is apart from absorbent filter tail
Hold 3~3.5cm.
Further, shell bottom upper surface is uniformly provided with 4-8 groove, and cap lower surface is provided with some relative with groove
The projection answered.
Further, the observation window and well are round rectangle.
Relative to prior art, the detection described in the utility model that MP non-specificity material and hemoglobin can be intercepted
Card has the advantage that:
(1) in the utility model on reagent strip interception line addition so that run film it is more preferable, background is cleaner, and technique is simple;
Interception line is anti-human RBC antibody, and the anti-human RBC antibody of fixed amount is added in sample pad, can be formed with antihuman hemoglobin
Compound, double intercepting systems of the anti-human RBC antibody in anti-human RBC antibody and interception line in sample pad, can be played completely
The effect of hemoglobin is intercepted, the covert utilization rate for improving sample, raw material and cost of labor is reduced, optimizes product
Energy.
(2) hole site is loaded in the utility model and adds one layer of treated glass fibre element film, can effectively intercept MP
The nonspecific interference material run into product, reduces false positive rate, improves the degree of accuracy, and such a method has equally been saved anti-
Interfering material, improve its utilization rate, cost declines.
Brief description of the drawings
Form a part of accompanying drawing of the present utility model to be used for providing further understanding to of the present utility model, this practicality is new
The schematic description and description of type is used to explain the utility model, does not form to improper restriction of the present utility model.
In accompanying drawing:
Fig. 1 is the card for the detection card that MP non-specificity material and hemoglobin can be intercepted described in the utility model embodiment
Shell structure schematic diagram;
Fig. 2 is the shell for the detection card that MP non-specificity material and hemoglobin can be intercepted described in the utility model embodiment
Bottom structure schematic diagram;
Fig. 3 is the detection card Ka Gai that MP non-specificity material and hemoglobin can be intercepted described in the utility model embodiment
Structural representation;
Fig. 4 is the examination for the detection card that MP non-specificity material and hemoglobin can be intercepted described in the utility model embodiment
The structural representation of agent bar.
Description of reference numerals:
1- caps;2- shells bottom;3- reagent strips;4- necks;5- observation windows;6- wells;7- glass fibre element films;8-PVC
Big plate;9- nitrocellulose filters;10- gold pads;11- sample pads;12- absorbent filters;13- interception lines;14- nature controlling lines;15- is detected
Line;16- grooves;17- is raised.
Embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the invention can
To be mutually combined.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " on ", " under ",
The orientation or position relationship of the instruction such as "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are
Based on orientation shown in the drawings or position relationship, it is for only for ease of description the invention and simplifies description, rather than instruction
Or imply that signified device or element must have specific orientation, with specific azimuth configuration and operation, therefore be not understood that
For the limitation to the invention.In addition, term " first ", " second " etc. are only used for describing purpose, and it is not intended that instruction
Or imply relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, " first ", " second " etc. are defined
Feature can express or implicitly include one or more this feature.In the description of the invention, unless separately
It is described, " multiple " are meant that two or more.
, it is necessary to which explanation, unless otherwise clearly defined and limited, term " are pacified in the description of the invention
Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integratedly
Connection;Can be mechanical connection or electrical connection;Can be joined directly together, can also be indirectly connected by intermediary,
It can be the connection of two element internals.For the ordinary skill in the art, on being understood by concrete condition
State concrete meaning of the term in the invention.
Describe the utility model in detail below with reference to the accompanying drawings and in conjunction with the embodiments.
As Figure 1-4, a kind of detection card that can intercept MP non-specificity material and hemoglobin, the detection Ka Bao
Include and get stuck and reagent strip 3, described to get stuck including cap 1 and shell bottom 2, the cap 1 is covered on the top of shell bottom 2, on the shell bottom 2
Surface is provided with the neck 4 for being used for placing reagent strip 3, and the upper surface of shell bottom 2 is uniformly provided with 6 grooves 16, and the lower surface of cap 1 is set
There are some projections 17 corresponding with groove 16.The upper surface of cap 1 is provided with observation window 5 and well 6, the observation window 5
It is round rectangle with well 6.Glass fibre element film 7 is provided with the well 6.
The reagent strip 3 includes the big plates 8 of PVC, and the big surfaces of plate 8 of PVC are provided with sticky paper, and sticky paper surface mount has nitrocellulose
Film 9, gold pad 10, sample pad 11 and absorbent filter 12, the head end of the gold pad 10 are overlapped in the head end of nitrocellulose filter 9, institute
It is 1.5mm to state gold pad 10 and be overlapped in the length on the surface of nitrocellulose filter 9.The head end of the sample pad 11 is overlapped in gold pad 10
Tail end, the head end of the absorbent filter 12 are overlapped in the tail end of nitrocellulose filter 9, and the surface of nitrocellulose filter 9 is along liquid
Body chromatography direction is coated with detection line 15 and nature controlling line 14 successively, and the nature controlling line 14 is apart from the tail end 2.5cm of absorbent filter 12, institute
Detection line 15 is stated apart from the tail end 3cm of absorbent filter 12.Anti-human RBC antibody, the nitrocellulose are provided with the sample pad 11
The surface of film 9 is also coated with interception line 13, and the interception line 13 is between detection line 15 and gold pad 10.The interception line 13 is anti-
People's RBC antibody.The interception line 13 is apart from the tail end 3.7cm of absorbent filter 12.
It is as follows to detect card each several part The concrete specification:
The big plates 8 of PVC:30cm*6cm;Sticky paper on the big plates 8 of PVC is divided into three sections of upper, middle and lower, the specification of three sections of sticky papers of upper, middle and lower
To be respectively 30cm*1.9cm, 30cm*2cm, 30cm*2.1cm;
Nitrocellulose filter 9:Width is 2.5cm;
Absorbent filter 12:Width is 1.9cm;
Gold pad 10:Width is 1cm;
Sample pad 11:30cm*1.7cm;
Get stuck:Get stuck overall 7cm*2cm;Observation window 5:1.6mm*3mm;Well 6:3mm*6mm;
Neck 4:3mm*6cm;
Reagent strip 3:6cm*3mm;
The nature controlling line 14 is apart from the tail end 2.5cm of absorbent filter 12;
The detection line 15 is apart from the tail end 3cm of absorbent filter 12;
The interception line 13 is apart from the tail end 3.7cm of absorbent filter 12.
Implementation process of the present utility model is:
Whole blood sample passes through the glass fibre element film 7 of well 6, filters out nonspecific interference thing present in sample
Matter, if continuing the normal anti-human RBC antibody for running sample, fixed amount being provided with sample pad 11 without if, by the filter of sample pad 11
After blood system, it is then passed through gold pad 10 and reaches nitrocellulose filter 9, interception line 13 is reached, if unnecessary hemoglobin does not have
It is removed, then can be intercepted under the gear of line 13, form the compound of anti-human RBC antibody-human hemoglobin, remaining composition continues to prolong nitre
The chromatography of acid cellulose film 9 arrives detection line 15 and nature controlling line 14, is finally adsorbed by absorbent filter 12, completes to run sample, finally reads letter
Number value.
Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model
Within the spirit and principle of utility model, any modification, equivalent substitution and improvements made etc., the utility model should be included in
Protection domain within.
Claims (7)
- A kind of 1. detection card that can intercept MP non-specificity material and hemoglobin, it is characterised in that:The detection card includes Get stuck and reagent strip (3);Described to get stuck including cap (1) and shell bottom (2), the cap (1) is covered on shell bottom (2) top, the cap (1) it is upper Surface is provided with observation window (5) and well (6), and glass fibre element film (7) is provided with the well (6);The shell bottom (2) Upper surface is provided with the neck (4) for being used for placing reagent strip (3);The reagent strip (3) includes the big plates of PVC (8), and the big plates of PVC (8) surface is provided with sticky paper, and the surface mount of the sticky paper has nitre Acid cellulose film (9), gold pad (10), sample pad (11) and absorbent filter (12), the head end of the gold pad (10) are overlapped in nitric acid The head end of cellulose membrane (9), the head end of the sample pad (11) are overlapped in the tail end of gold pad (10), the absorbent filter (12) Head end is overlapped in the tail end of nitrocellulose filter (9);Wrapped successively along liquid chromatography(LC) direction on the surface of the nitrocellulose filter (9) There are interception line (13), detection line (15) and nature controlling line (14), anti-human RBC antibody is provided with the sample pad (11).
- 2. the detection card according to claim 1 that MP non-specificity material and hemoglobin can be intercepted, it is characterised in that: The interception line (13) is anti-human RBC antibody.
- 3. the detection card according to claim 1 that MP non-specificity material and hemoglobin can be intercepted, it is characterised in that: The interception line (13) is apart from absorbent filter (12) 3~4cm of tail end.
- 4. the detection card that can intercept MP non-specificity material and hemoglobin according to claim any one of 1-3, its It is characterised by:The length that the gold pad (10) is overlapped in nitrocellulose filter (9) surface is 1-1.5mm.
- 5. the detection card according to claim 4 that MP non-specificity material and hemoglobin can be intercepted, it is characterised in that: The nature controlling line (14) is apart from 2~2.5cm of tail end of absorbent filter (12), and the detection line (15) is apart from absorbent filter (12) 3~3.5cm of tail end.
- 6. the detection card according to claim 4 that MP non-specificity material and hemoglobin can be intercepted, it is characterised in that: The upper surface of the shell bottom (2) is uniformly provided with 4-8 groove (16), and the lower surface of cap (1) is provided with some with groove (16) phase Corresponding projection (17).
- 7. the detection card according to claim 1 that MP non-specificity material and hemoglobin can be intercepted, it is characterised in that: The observation window (5) and well (6) are round rectangle.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109459567A (en) * | 2018-12-26 | 2019-03-12 | 北京康思润业生物技术有限公司 | Immuno-chromatographic test paper strip and immuno-chromatography detection device including it |
CN109459566A (en) * | 2018-12-26 | 2019-03-12 | 北京康思润业生物技术有限公司 | Immuno-chromatographic test paper strip and immuno-chromatography detection device including it |
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2017
- 2017-05-19 CN CN201720563142.8U patent/CN206740777U/en active Active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109459567A (en) * | 2018-12-26 | 2019-03-12 | 北京康思润业生物技术有限公司 | Immuno-chromatographic test paper strip and immuno-chromatography detection device including it |
CN109459566A (en) * | 2018-12-26 | 2019-03-12 | 北京康思润业生物技术有限公司 | Immuno-chromatographic test paper strip and immuno-chromatography detection device including it |
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