CN206671144U - A kind of improved fluidic device nozzle - Google Patents
A kind of improved fluidic device nozzle Download PDFInfo
- Publication number
- CN206671144U CN206671144U CN201720250352.1U CN201720250352U CN206671144U CN 206671144 U CN206671144 U CN 206671144U CN 201720250352 U CN201720250352 U CN 201720250352U CN 206671144 U CN206671144 U CN 206671144U
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- China
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- confluxes
- confluxing
- chamber
- plug
- sheath fluid
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Abstract
It the utility model is related to flow cytometer, specifically a kind of improved fluidic device nozzle, conflux chamber including taper, sheath fluid and testing sample conflux focusing since the big end in chamber top of confluxing, the bottom small end of the chamber that confluxes forms shower nozzle, the plug that confluxes is provided with the middle part of the chamber that confluxes, conflux and offer several holes of confluxing beyond the Great Wall, intracavitary of confluxing on the downside of the plug that confluxes is connected with sheath fluid replenisher, sheath fluid and testing sample conflux focusings again after several holes of confluxing are confluxed and focused on the sheath fluid replenisher of downside, then from shower nozzle outflow.The utility model is provided with the plug that confluxes with hole of confluxing in the intracavitary of confluxing of nozzle, after sheath fluid and testing sample, which enter, confluxes chamber, preliminary focusing of confluxing first is carried out in the upside for the plug that confluxes, then confluxed again focusing by the hole of confluxing for the plug that confluxes, so as to improve focusing effect, and then improve measurement accuracy.
Description
Technical field
Flow cytometer is the utility model is related to, specifically a kind of improved fluidic device nozzle.
Background technology
At present, cell or particulate are detected and analyzed generally has two class methods：One kind is static method, i.e., to be measured
Sample（Containing cell or the liquid of other particulates or lamellar tissue）After doing single or multiple lift processing, it is statically compressed on thin
On piece（Such as slide）, then its various performance characterization parameter is detected and analyzed, the instrument of use generally has ordinary optical
Microscope, fluorescence microscope or laser confocal microscope etc.；Another kind of is dynamic method, that is, is allowed thin in testing sample
Born of the same parents or other particulates flow through specific search coverage " one by one " with constant speed, utilize detection device（Such as photomultiplier,
Avalanche diode etc.）Each cell of the search coverage or the multiple performance characterization parameter of other particulates, and profit are flowed through in observation
Data processing on a large scale and classification are done to the result of observation with computer, here it is the operation principle of flow cytometer.
Flow cytometer can carry out multi-parameter, quantitative analysis to the cell or biological particle being under quick flow regime
Or sorting, the basic research such as cell biology, immunology, physiology, molecular biology are widely used to, while be also used for
The clinical diagnosis of medical science, environment measuring etc..One of the critical component of flow cytometer nozzle as flow cytometer, mainly
Effect be by the cell in sample suspensions or other particulates can single-row layout, successively in order by laser detection area.Nozzle profit
With hydrodynamics focusing principle, sheath fluid and testing sample are converged in intracavitary of confluxing, sample flow is formed stable laminar flow, this
When, sheath fluid is enclosed in around sample flow, and sample flow forms stable streamlined flow liquid by laser detection area, for optical system
System detection and analysis.The chamber that confluxes of existing nozzle causes accuracy of detection because the focusing effect to sheath fluid and testing sample stream is bad
Not enough.
Utility model content
For above-mentioned technical problem, the utility model provides a kind of improved fluidic device spray for improving focusing effect
Mouth.
Technical scheme is used by the utility model solves above-mentioned technical problem：A kind of improved fluidic device nozzle,
Conflux chamber including taper, sheath fluid and testing sample conflux focusing since the big end in chamber top of confluxing, the bottom small end of the chamber that confluxes
Shower nozzle is formed, the plug that confluxes is provided with the middle part of the chamber that confluxes, confluxes and offer several holes of confluxing beyond the Great Wall, the remittance on the downside of the plug that confluxes
Stream intracavitary is connected with sheath fluid replenisher, sheath fluid and testing sample after several holes of confluxing are confluxed and focused on the sheath fluid replenisher of downside again
Secondary focusing of confluxing, then flowed out from the shower nozzle.
Preferably, the chamber side wall of confluxing is provided with the runner communicated with the chamber that confluxes positioned at plug downside of confluxing, it is described
Sheath fluid replenisher enters the chamber that confluxes by the runner.
Preferably, the appearance profile of the plug that confluxes is molded according to the cavity shape for the chamber that confluxes.
Preferably, each hole of confluxing is in the taper that upper end is big, lower end is small.
Preferably, the plug that confluxes is made of elastomeric material.
As can be known from the above technical solutions, the utility model is provided with confluxing with hole of confluxing in the intracavitary of confluxing of nozzle
Plug, after sheath fluid enters with testing sample confluxes chamber, preliminary focusing of confluxing first is carried out in the upside for the plug that confluxes, then by confluxing
The hole of confluxing of plug is confluxed focusings again, so as to improve focusing effect, and then raising measurement accuracy.
Brief description of the drawings
Fig. 1 is a kind of structural representation of preferred embodiment of the present utility model.
Embodiment
The utility model is discussed in detail with reference to Fig. 1, illustrative examples of the present utility model and explanation are used herein
To explain the utility model, but it is not intended as to restriction of the present utility model.
A kind of flow cytometer nozzle, including taper are confluxed chamber 1, and sheath fluid is with testing sample since the big end in chamber top of confluxing
Conflux focusing, the bottom small end of the chamber that confluxes forms shower nozzle 2, is provided with the plug 3 that confluxes in the middle part of the chamber that confluxes, confluxes and offer beyond the Great Wall
Several holes 4 of confluxing, the intracavitary of confluxing on the downside of the plug that confluxes are connected with sheath fluid replenisher, and sheath fluid is with testing sample first in the upper of the plug that confluxes
Side carries out preliminary focusings of confluxing, and is then divided into the hole of confluxing that several bursts of sample flows flow into the plug that confluxes, due to remittance orifice aperture very little, its
Sample flow can fully be compressed, the focusing so that sample flow is confluxed again, sample flow passes through the sheath on the downside of the Kong Houyu that confluxes
Liquid replenisher carries out third time and confluxed focusing, so as to ensure that sample flow fully focuses on, sample flow by focusing of confluxing three times from
The shower nozzle outflow, and then improve the purpose of focusing effect.Detection zone 5 is entered back into from the sample flow of shower nozzle outflow to enter
Row detection.
In implementation process, the chamber side wall of confluxing is provided with the runner 6 communicated with the chamber that confluxes positioned at plug downside of confluxing,
Several can be set in runner, converge sample basin sheath fluid replenisher more uniform, the sheath fluid replenisher is entered by the runner
Conflux chamber, so as to carrying out focusing of confluxing from the sample for hole outflow of confluxing, on the one hand be to focus on incomplete testing sample to mend
Focusing is filled, on the other hand jet pressures is supplemented for shower nozzle, can not only improve accuracy of detection, can also improve detection efficiency.
The appearance profile of the plug of the present utility model that confluxes is molded according to the cavity shape for the chamber that confluxes, and guarantee, which is confluxed, fills in and the chamber that confluxes
Cooperation it is preferable, be not in gap, avoid sample flow from flowing to shower nozzle from gap；And the plug that confluxes is arranged on the middle part for the chamber that confluxes,
So cause sheath fluid and testing sample there are more focal times that conflux in side beyond the Great Wall of confluxing, improve the effect of preliminary focusing of confluxing
Fruit, and the cavity for plug bottom of confluxing converges several bursts of sample flows, enough pressure can be provided for shower nozzle, so as to improve detection efficiency；
Each hole of confluxing can further improve focusing of confluxing in the taper that upper end is big, lower end is small；The plug that confluxes uses elastomeric material system
Into convenient for assembly and dismounting.
Above-mentioned embodiment is only for illustration of the utility model, and is not the relevant skill to limitation of the present utility model
The those of ordinary skill in art field, can be so that various changes can be made in the case where not departing from the spirit and scope of the utility model
And modification, therefore all equivalent technical schemes should also belong to category of the present utility model.
Claims (5)
1. a kind of improved fluidic device nozzle, including taper is confluxed chamber, and sheath fluid is opened with testing sample from the big end in chamber top of confluxing
Beginning confluxes focusings, the bottom small end formation shower nozzle of the chamber that confluxes, it is characterised in that：The plug that confluxes is provided with the middle part of the chamber that confluxes,
Conflux and offer several holes of confluxing beyond the Great Wall, the intracavitary of confluxing on the downside of the plug that confluxes is connected with sheath fluid replenisher, sheath fluid and testing sample
Conflux again with the sheath fluid replenisher of downside after several holes of confluxing are confluxed and focused on focusings, then from shower nozzle outflow.
2. fluidic device nozzle according to claim 1, it is characterised in that：The chamber side wall of confluxing is set positioned at plug downside of confluxing
The runner communicated with the chamber that confluxes is equipped with, the sheath fluid replenisher enters the chamber that confluxes by the runner.
3. fluidic device nozzle according to claim 1, it is characterised in that：The appearance profile of the plug that confluxes is according to the chamber that confluxes
Cavity shape is molded.
4. according to the 1 or 2 or 3 fluidic device nozzle of claim, it is characterised in that：It is each it is described conflux hole in upper end it is big,
The small taper in lower end.
5. according to the 1 or 2 or 3 fluidic device nozzle of claim, it is characterised in that：The plug that confluxes uses elastomeric material system
Into.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720250352.1U CN206671144U (en) | 2017-03-16 | 2017-03-16 | A kind of improved fluidic device nozzle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720250352.1U CN206671144U (en) | 2017-03-16 | 2017-03-16 | A kind of improved fluidic device nozzle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206671144U true CN206671144U (en) | 2017-11-24 |
Family
ID=60377362
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720250352.1U Expired - Fee Related CN206671144U (en) | 2017-03-16 | 2017-03-16 | A kind of improved fluidic device nozzle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206671144U (en) |
-
2017
- 2017-03-16 CN CN201720250352.1U patent/CN206671144U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171124 Termination date: 20190316 |