CN206540804U - The nozzle of flow cytometer fluidic device - Google Patents
The nozzle of flow cytometer fluidic device Download PDFInfo
- Publication number
- CN206540804U CN206540804U CN201720249004.2U CN201720249004U CN206540804U CN 206540804 U CN206540804 U CN 206540804U CN 201720249004 U CN201720249004 U CN 201720249004U CN 206540804 U CN206540804 U CN 206540804U
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- China
- Prior art keywords
- confluxing
- confluxes
- nozzle
- chamber
- plug
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Abstract
The utility model is related to flow cytometer, the specifically nozzle of flow cytometer fluidic device, conflux chamber including taper, sheath fluid and testing sample conflux focusing since the big end in chamber top of confluxing, the bottom small end formation shower nozzle of the chamber that confluxes, the intracavitary of confluxing is provided with the plug that confluxes, confluxes and offer several holes of confluxing beyond the Great Wall, it is connected with sheath fluid replenisher in each hole of confluxing, sheath fluid and its replenisher and testing sample conflux through several holes of confluxing after focusing to flow out from the shower nozzle.The utility model is provided with the plug that confluxes with hole of confluxing in the intracavitary of confluxing of nozzle, after sheath fluid and testing sample, which enter, confluxes chamber, preliminary focusing of confluxing first is carried out in the upside for the plug that confluxes, then confluxed again focusing by the hole of confluxing for the plug that confluxes, so as to improve focusing effect, and then improve measurement accuracy.
Description
Technical field
The utility model is related to flow cytometer, specifically the nozzle of flow cytometer fluidic device.
Background technology
At present, cell or particulate are detected and analyzed generally has two class methods：One class is static method, i.e., to be measured
Sample（Liquid containing cell or other particulates or lamellar tissue）Do after single or multiple lift processing, it is statically compressed on thin
On piece（Such as slide）, then its various performance characterization parameter is detected and analyzed, the instrument of use generally has ordinary optical
Microscope, fluorescence microscope or laser confocal microscope etc.；Another kind of is dynamic method, that is, allows thin in testing sample
Born of the same parents or other particulates flow through specific search coverage with constant speed " one by one ", utilize detection device（Such as photomultiplier,
Avalanche diode etc.）Each cell of the search coverage or the multiple performance characterization parameter of other particulates, and profit are flowed through in observation
Data processing on a large scale and classification are done to the result of observation with computer, here it is the operation principle of flow cytometer.
Flow cytometer can carry out multi-parameter, quantitative analysis to the cell or biological particle being under quick flow regime
Or sorting, the basic research such as cell biology, immunology, physiology, molecular biology are widely used to, while being also used for
In terms of the clinical diagnosis of medical science, environment measuring.Flow cytometer nozzle is as one of critical component of flow cytometer, mainly
Effect be by the cell in sample suspensions or other particulates can single-row layout, successively in order by laser detection area.Nozzle profit
Hydrodynamics focusing principle is used, by sheath fluid and testing sample in intracavitary convergence of confluxing, sample stream is formed stable laminar flow, this
When, sheath fluid is enclosed in around sample stream, and sample stream forms stable streamlined flow liquid by laser detection area, for optical system
System detection and analysis.The chamber that confluxes of existing nozzle causes accuracy of detection because the focusing effect to sheath fluid and testing sample stream is bad
Not enough.
Utility model content
For above-mentioned technical problem, the utility model provides a kind of flow cytometer fluidic device for improving focusing effect
Nozzle.
The utility model solve the technical scheme that is used of above-mentioned technical problem for：The spray of flow cytometer fluidic device
Mouth, including taper are confluxed chamber, and sheath fluid and testing sample conflux focusing since the big end in chamber top of confluxing, and the bottom of the chamber that confluxes is small
End forms shower nozzle, and the intracavitary of confluxing is provided with the plug that confluxes, and confluxes and offers several holes of confluxing beyond the Great Wall, leads in each hole of confluxing
There is a sheath fluid replenisher, sheath fluid and its replenisher and testing sample conflux through several holes of confluxing after focusing to flow out from the shower nozzle.
Preferably, confluxing beyond the Great Wall, several holes of confluxing form several rows, conflux beyond the Great Wall along its cross section in terms of open up
Several are connected with the runner of sheath fluid replenisher, and each hole of confluxing that a runner confluxes with a corresponding row in hole is communicated.
Preferably, on the outside of the chamber that confluxes, several runners pool one.
Preferably, the appearance profile of the plug that confluxes is molded according to the cavity shape for the chamber that confluxes.
Preferably, the plug that confluxes is arranged on the middle part for the chamber that confluxes.
Preferably, each hole of confluxing is in the taper that upper end is big, lower end is small.
Preferably, the plug that confluxes is made of elastomeric material.
As can be known from the above technical solutions, the utility model is provided with confluxing with hole of confluxing in the intracavitary of confluxing of nozzle
Plug, after sheath fluid and testing sample, which enter, confluxes chamber, first carries out preliminary focusing of confluxing, then by confluxing in the upside for the plug that confluxes
The hole of confluxing of plug is confluxed focusings again, so that focusing effect is improved, and then raising measurement accuracy.
Brief description of the drawings
Fig. 1 is a kind of structural representation of preferred embodiment of the present utility model.
Fig. 2 is the diagrammatic cross-section of plug and runner of confluxing in the utility model.
Embodiment
The utility model is discussed in detail with reference to Fig. 1, Fig. 2, illustrative examples of the present utility model and says herein
It is bright to be used for explaining the utility model, but be not intended as limiting of the present utility model.
A kind of nozzle of flow cytometer fluidic device, including taper are confluxed chamber 1, and sheath fluid is with testing sample from the chamber that confluxes
The big end in portion starts focusing of confluxing, and the bottom small end formation shower nozzle 2 of the chamber that confluxes, the intracavitary of confluxing is provided with the plug 3 that confluxes, confluxed
Offer beyond the Great Wall in several holes 4 of confluxing, each hole of confluxing and be connected with sheath fluid replenisher, testing sample is confluxed in narrow in hole
Preferably focus on；In implementation process, sheath fluid first carries out preliminary conflux focusing, Ran Houfen with testing sample in the upside for the plug that confluxes
The hole of confluxing for the plug that confluxes is flowed into several strands of sample streams, due to remittance orifice aperture very little, it can fully be compressed to sample stream, and
Conflux and be also connected with sheath fluid replenisher in hole, the focusing so that sample stream fully confluxes again reaches the purpose for improving focusing effect.
Sample stream, from shower nozzle outflow, is detected after focusing is confluxed in several holes of confluxing into detection zone 5.
Conflux beyond the Great Wall of the present utility model, several holes of confluxing form several rows, conflux beyond the Great Wall along its cross section in terms of open
If several are connected with the runner 6 of sheath fluid replenisher, each hole of confluxing that a runner confluxes with a corresponding row in hole is communicated,
So as to ensure that in each hole of confluxing replenisher can be passed through.In implementation process, runner may be provided at the middle part for the plug that confluxes, and be conducive to
Focus on.On the outside of the chamber that confluxes, several runners pool one, are conducive to conveying sheath fluid replenisher, process more convenient.
The appearance profile of the plug of the present utility model that confluxes is molded according to the cavity shape for the chamber that confluxes, it is ensured that conflux plug and the chamber that confluxes
Cooperation preferably, be not in gap, it is to avoid sample stream flows to shower nozzle from gap；The plug that confluxes is arranged in the chamber that confluxes
Portion, so that sheath fluid has more focal times that conflux in side beyond the Great Wall of confluxing with testing sample, improves preliminary focusing of confluxing
Effect, and the cavity for plug bottom of confluxing converges several strands of sample streams, and enough pressure can be provided for shower nozzle, so as to improve detection effect
Rate；Each hole of confluxing can further improve focusing of confluxing in the taper that upper end is big, lower end is small；Plug conflux using elastic material
Material is made, convenient for assembly and dismounting.
Above-mentioned embodiment is only for illustration of the utility model, and is not the relevant skill to limitation of the present utility model
The those of ordinary skill in art field, can be so that various changes can be made in the case where not departing from the utility model spirit and scope
And modification, therefore all equivalent technical schemes should also belong to category of the present utility model.
Claims (7)
1. the nozzle of flow cytometer fluidic device, including taper is confluxed chamber, and sheath fluid and testing sample are from the big end in chamber top of confluxing
Start focusing of confluxing, the bottom small end formation shower nozzle of the chamber that confluxes, it is characterised in that：The intracavitary of confluxing is provided with the plug that confluxes,
Conflux and offer several holes of confluxing beyond the Great Wall, sheath fluid replenisher is connected with each hole of confluxing, sheath fluid and its replenisher are with treating test sample
Product through several holes of confluxing conflux focusing after from the shower nozzle flow out.
2. the nozzle of fluidic device according to claim 1, it is characterised in that：Confluxing beyond the Great Wall, several holes of confluxing form number
Row, conflux beyond the Great Wall along its cross section in terms of opened up several runners for being connected with sheath fluid replenisher, a runner with it is corresponding
Each hole of confluxing that one row is confluxed in hole is communicated.
3. the nozzle of fluidic device according to claim 2, it is characterised in that：On the outside of the chamber that confluxes, several runners are pooled
One.
4. the nozzle of fluidic device according to claim 1 or 2 or 3, it is characterised in that：The appearance profile of the plug that confluxes according to
Conflux chamber cavity shape shaping.
5. the nozzle of fluidic device according to claim 4, it is characterised in that：The plug that confluxes is arranged in the chamber that confluxes
Portion.
6. the nozzle of fluidic device according to claim 1 or 2 or 3, it is characterised in that：Each hole of confluxing is in upper end
Greatly, the small taper in lower end.
7. the nozzle of fluidic device according to claim 1 or 2 or 3, it is characterised in that：The plug that confluxes uses elastomeric material
It is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720249004.2U CN206540804U (en) | 2017-03-16 | 2017-03-16 | The nozzle of flow cytometer fluidic device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720249004.2U CN206540804U (en) | 2017-03-16 | 2017-03-16 | The nozzle of flow cytometer fluidic device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206540804U true CN206540804U (en) | 2017-10-03 |
Family
ID=59943240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720249004.2U Expired - Fee Related CN206540804U (en) | 2017-03-16 | 2017-03-16 | The nozzle of flow cytometer fluidic device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206540804U (en) |
-
2017
- 2017-03-16 CN CN201720249004.2U patent/CN206540804U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171003 Termination date: 20190316 |
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| CF01 | Termination of patent right due to non-payment of annual fee |