CN105728069A - Multi-channel micro-fluidic chip for rapid blood self-inspection - Google Patents
Multi-channel micro-fluidic chip for rapid blood self-inspection Download PDFInfo
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- CN105728069A CN105728069A CN201610068754.XA CN201610068754A CN105728069A CN 105728069 A CN105728069 A CN 105728069A CN 201610068754 A CN201610068754 A CN 201610068754A CN 105728069 A CN105728069 A CN 105728069A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
Abstract
The invention discloses a multi-channel micro-fluidic chip for rapid blood self-inspection.The chip comprises a substrate and a cover plate.A sample introduction channel, an inertia separation channel, a flow distribution channel, a plasma analysis channel and a blood cell analysis channel.The sample introduction channel, the inertia separation channel and the flow distribution channel are connected in sequence.The flow distribution cannel is of a Y-shaped structure to be connected to the plasma analysis channel and the blood cell analysis channel.The sample introduction channel is used for inputting blood to be inspected.The inertia separation channel separates blood into plasma and blood cells, and the plasma and the blood cells flow into the flow distribution channel.The flow distribution channel introduces the plasma into the plasma analysis channel through the Y-shaped structure for detecting and analyzing the plasma, and introduces the blood cells into the blood cell analysis channel for detecting and analyzing the blood cells.The highly-integrated multi-channel micro-fluidic chip is adopted, and the function of detecting various kinds of blood is achieved at a time on the basis that carrying is convenient.
Description
Technical field
The present invention relates to blood testing technical field, particularly relate to a kind of multichannel micro-fluidic chip for quick self-checking blood.
Background technology
Micro-fluidic chip be one integrate blood sample prepare, react, separate, the small-sized analytic experiment porch of the function such as detection.Microfluidic chip analysis completes to automatically analyze overall process with the chip of micro-meter scale for operating platform, its device characteristic mainly has the resulting structure holding fluid, such as passage or reative cell, there is powerful integration, it is light and handy that its advantage includes volume, use sample is few, response speed is fast, high flux, low stain, instant abandon, this makes it at biology, chemistry, medical science, electronics, the research field of the subject crossing such as machinery shows huge development potentiality, for biomedical research, pharmaceutical synthesis screens, environmental monitoring and protection, health quarantine, judicial expertise, the various fields such as the detection of biological reagent.
Micro-fluidic chip can carry multiple monotechnics and so as to flexible combination and the integrated feature of scale become the Important Platform of various systematic study, and the application in cytobiology constantly extends, and is the future thrust of biological medicine analysis field.Along with now social development, the idea of family healthcare deepens continuously the popular feeling, various household medical products are constantly released, but this basic health examination content of blood testing still relies on the diagnostic equipment of hospital, these diagnostic equipments cannot realize disposable multiple blood testing function, and armarium volume is very much not easy to carry, thus giving family's self-inspection blood operating difficulties, bring inconvenience.
Summary of the invention
Present invention is primarily targeted at a kind of multichannel micro-fluidic chip for quick self-checking blood of offer, it is intended to solve existing diagnostic equipment and cannot realize disposable multiple blood testing function and problem inconvenient to carry.
For achieving the above object, the invention provides a kind of multichannel micro-fluidic chip for quick self-checking blood, including substrate and cover plate, described cover plate is integrated with sample intake passage, inertial separation passage, split channel, plasma analysis passage and blood cell analysis passage, described sample intake passage, inertial separation passage and split channel are sequentially connected, described split channel adopts y-type structure to be connected to described plasma analysis passage and described blood cell analysis passage, wherein:
Described sample intake passage is used for inputting blood to be checked；
Described blood separation is blood plasma and hemocyte by described inertial separation passage, and described blood plasma and hemocyte flow into described split channel；
Blood plasma is carried out detection by described y-type structure and analyzes by the described blood plasma described plasma analysis passage of introducing by described split channel, and hemocyte is carried out detection analysis by the described hemocyte described blood cell analysis passage of introducing.
Preferably, being also integrated with waste fluid channel in described cover plate, described plasma analysis passage is all connected with described waste fluid channel with blood cell analysis passage, and described waste fluid channel is for deriving the waste liquid that the blood plasma analyzed and hemocyte produce.
Preferably, described plasma analysis passage includes hepatitis A analysis platform, hepatitis B analysis platform, arthritis analysis platform and myocardial infarction analysis platform.
Preferably, described hepatitis A analysis platform includes IgM probe unit and IgA probe unit；Described hepatitis B analysis platform includes HBsAg probe unit, HBsAb probe unit, HBeAg probe unit, HBeAb probe unit and HBcAb probe unit；Described arthritis analysis platform includes RF-IgM probe unit, RF-IgA probe unit, RF-IgG probe unit and RF-IgE probe unit；Described myocardial infarction analysis platform includes hs-CRP probe unit and CK-MB probe unit；Described immunoassay platform is by comprehensively its each internal probe unit testing result detecting data judging blood plasma to blood plasma.
Preferably, described blood cell analysis passage includes platelet count platform, red blood cell count(RBC) platform, hemoglobin analysis platform, numeration of leukocyte platform and blood type analysis platform.
Preferably, described platelet count platform includes platelet sieve and platelet counter；Described red blood cell count(RBC) platform includes erythrocyte sieve, erythrocyte drainage tube and erythrocytometer；Described platelet count platform isolates the platelet in hemocyte by controlling the line size of described platelet sieve, and logical platelet counter counts；Described red blood cell count(RBC) platform isolates the erythrocyte in hemocyte by controlling the line size of described erythrocyte sieve, and is counted by erythrocytometer；Described numeration of leukocyte platform is isolated the leukocyte in hemocyte by controlling the line size of self and counts.
Preferably, when hemocyte flows through described blood cell analysis passage, platelet is trapped in described platelet count platform, erythrocyte is through described red blood cell count(RBC) platform, entered described hemoglobin analysis platform by described erythrocyte drainage tube and carry out hemoglobin separation determination, remaining leukocyte is counted by described numeration of leukocyte platform, and enters described blood type analysis platform and carry out blood group gene type analysis.
Preferably, described hemoglobin analysis platform includes erythrocyte splitting unit, hemoglobin test cell and valve, wherein:
Described erythrocyte splitting unit and described hemoglobin test cell are disc structure, and both are vertically arranged；
Described valve is arranged between described erythrocyte splitting unit and described hemoglobin test cell；
Described erythrocyte splitting unit is provided with hemolytic agent.
Preferably, described sample intake passage, inertial separation passage, split channel, plasma analysis passage, blood cell analysis passage and waste fluid channel all adopt cambered structure.
Compared to prior art, multichannel micro-fluidic chip for quick self-checking blood of the present invention have employed technique scheme, reach following beneficial effect: this multichannel micro-fluidic chip is by integrated sample intake passage, separated region, split channel, analysis platform and waste fluid channel on the chip of centimeter scale, realize disposable multiple blood testing function, and volume is little is easy to carry, family's self-inspection is facilitated to use.
Accompanying drawing explanation
Fig. 1 is the present invention structural representation for the multichannel micro-fluidic chip preferred embodiment of quick self-checking blood；
Fig. 2 is the present invention structural representation for the inertial separation passage preferred embodiment of the multichannel micro-fluidic chip of quick self-checking blood；
Fig. 3 is the present invention structural representation for the plasma analysis passage preferred embodiment of the multichannel micro-fluidic chip of quick self-checking blood；
Fig. 4 is the present invention structural representation for the blood cell analysis passage preferred embodiment of the multichannel micro-fluidic chip of quick self-checking blood；
Fig. 5 is the present invention structural representation for the hemoglobinometry platform preferred embodiment of the multichannel micro-fluidic chip of quick self-checking blood.
The object of the invention realization, functional characteristics and advantage will in conjunction with the embodiments, are described further with reference to accompanying drawing.
Detailed description of the invention
For further setting forth that the present invention reaches technological means and effect that above-mentioned purpose is taked, below in conjunction with accompanying drawing, the specific embodiment of the present invention, structure, feature and effect thereof are illustrated.It should be pointed out that, specific embodiment described herein is only in order to explain the present invention, do not limit the present invention in any form.
As it is shown in figure 1, Fig. 1 is the present invention structural representation for the multichannel micro-fluidic chip preferred embodiment of quick self-checking blood.In the present embodiment, described multichannel micro-fluidic chip includes substrate 1 and cover plate 2, described cover plate 2 is passable, but it is not limited only to, it is made up of polydimethylsiloxane (PDMS), described cover plate 2 is integrated with the microfluidic channel structure realizing multiple blood examination function, including sample intake passage 21, inertial separation passage 22, split channel 23, plasma analysis passage 24, blood cell analysis passage 25 and waste fluid channel 26.Described sample intake passage 21 adopts open-celled structure to connect described inertial separation passage 22, and this sample intake passage 21 is for the input blood to be checked that is connected with blood transfusion tube.Described inertia channel 22 communicates with the entrance of described split channel 23.Described split channel 23 is y-type structure, and including an entrance and two outlets, two outlets of described split channel 23 connect described plasma analysis passage 24 and blood cell analysis passage 25 respectively.Described plasma analysis passage 24 includes the hepatitis A analysis platform 241, hepatitis B analysis platform 242, arthritis analysis platform 243 and the myocardial infarction analysis platform 244 that be arranged in parallel.Described blood cell analysis passage 25 includes platelet count platform 251, red blood cell count(RBC) platform 252, hemoglobin analysis platform 253, numeration of leukocyte platform 254 and blood type analysis platform 255.Described plasma analysis passage 24 is all connected with described waste fluid channel 26 with blood cell analysis passage 25, and the outlet of described waste fluid channel 26 is open-celled structure, for being derived by the waste liquid that the blood plasma analyzed and hemocyte produce.
As in figure 2 it is shown, Fig. 2 is the present invention structural representation for the inertial separation passage preferred embodiment of the multichannel micro-fluidic chip of quick self-checking blood.In the present embodiment, the blood flow volume of microliter quantity level is imported in described sample intake passage 21 by capillary vessel, blood to be checked is imported in described inertial separation passage 22 by described sample intake passage 21, and described blood separation is become blood plasma and hemocyte two parts based on inertia miniflow principle by described inertial separation passage 22.Blood plasma separation schematic diagram in the present embodiment is as shown in Figure 2, blood plasma and the separation of hemocyte in described blood are implemented in described inertial separation passage 22, the sectional view of described inertial separation passage 22 shows that described inertial separation passage 22 is asymmetric syphon shape structure, and described blood passes through liquid flowing in the duct through described asymmetric syphon shape structure and produces laminar flow.Unsymmetry based on pipeline, described blood produces the eddy current of a pair reverse rotation and symmetry when flowing in the duct, described eddy current is referred to as Dien eddy current, hemocyte in described blood is applied inertia lift by described laminar flow, described inertia lift makes hemocyte stably equilbrium position in cross-section, hemocyte in described blood is produced Dien drag force by described Dien eddy current, and described hemocyte flows under the combined effect of described inertia lift and Dien drag force.In the present embodiment, through designing size and the length of described asymmetric syphon shape structure, hemocyte only produces stress balance under suitable flow conditions in the side of channel cross-section, the flowing of described blood is that single bundle focuses on flowing, described blood is separated into two-layer flow liquid, described two-layer flow liquid respectively hemocyte and blood plasma through described inertial separation passage 22.In the lump with reference to shown in Fig. 1, under the shunting action of the asymmetrical Y-type structure of described split channel 23, described blood plasma enters described plasma analysis passage 24, passes sequentially through described hepatitis A analysis platform 241, hepatitis B analysis platform 242, arthritis analysis platform 243 and myocardial infarction analysis platform 244 and carries out detection of specific antibody；Described hemocyte is entered described blood cell analysis passage 25 by drain, passes sequentially through described platelet count platform 251, red blood cell count(RBC) platform 252, hemoglobin analysis platform 253, numeration of leukocyte platform 254 and blood type analysis platform 255.Blood plasma and hemocyte waste liquid after detecting by analysis are discharged each through described waste fluid channel 26.
As it is shown on figure 3, Fig. 3 is the present invention structural representation for the plasma analysis passage preferred embodiment in the multichannel micro-fluidic chip of quick self-checking blood.In the present embodiment, described hepatitis A analysis platform 241 includes IgM probe unit 2411 and IgA probe unit 2412, and described IgM probe unit 2411 and IgA probe unit 2412 are integrated in described micro-fluidic chip substrate 1.Described IgM probe unit 2411 is coated with HAV-Ag (HAV-Ag) immunity layer, described HAV-Ag is used for catching in blood plasma the anti-HAV-IgM of specificity as antigen, form antigen-antibody complex, Non-specific combination between described Ag-Ab produces electrochemical signals, and described electrochemical signals is by described IgM probe unit 2411 detection by quantitative, analysis and conduction.Described IgA probe unit 2412 is coated with anti-human μ chain immunity layer, described anti-human μ chain is used for catching in blood plasma specificity, and anti-HAV-IgA forms antigen-antibody complex and produces electrochemical signals, and described IgA probe unit 2412 is for the content of the anti-HAV-IgA in detection by quantitative, analysis and the described blood plasma of conduction.Described hepatitis A analysis platform 241 is by the Analysis of test results Hepatitis A infection level of comprehensive described IgM probe unit 2411 and IgA probe unit 2412.
Described hepatitis B analysis platform 242 includes hepatitis B surface antigen (HBsAg) probe unit 2421, hepatitis B surface antibody (HBsAb) probe unit 2422, hepatitis B virus e antigen (HBeAg) probe unit 2423, hepatitis B e antibody (HBeAb) probe unit 2424 and hepatitis B core antibody (HBcAb) probe unit 2425.By Ag-Ab specific recognition and the electrochemical signals combining generation, the content of HBsAg, HBsAb, HBeAg, HBeAb and HBcAb of being respectively used in detection by quantitative, analysis and conduction blood plasma on described HBsAg probe unit 2421, HBsAb probe unit 2422, HBeAg probe unit 2423, HBeAb probe unit 2424 and HBcAb probe unit 2425.Described myocardial infarction analysis platform 243 obtains the inspection result of five indexes of hepatitis b by the interpretation of result of comprehensive described HBsAg probe unit 2421, HBsAb probe unit 2422, HBeAg probe unit 2423, HBeAb probe unit 2424 and HBcAb probe unit 2425, judges hepatitis B infected situation according to this.
Described arthritis analysis platform 243 includes four rheumatoid factor (rheumatoidfactor, RF) probe unit, respectively RF-IgM probe unit 2431, RF-IgA probe unit 2432, RF-IgG probe unit 2433 and RF-IgE probe unit 2434.Described RF-IgM probe unit 2431, RF-IgA probe unit 2432, RF-IgG probe unit 2433 and RF-IgE probe unit 2434 are respectively used to specificity and catch IgM type in serum, IgG type IgA type and IgE type rheumatoid factor, and the electrochemical signals produced by forming antigen-antibody complex analyzes the content of all types of rheumatoid factor in described blood plasma.Described first arthritis analysis platform 243 judges the situation of rheumatoid arthritis by the testing result of comprehensive described RF-IgM probe unit 2431, RF-IgA probe unit 2432, RF-IgG probe unit 2433 and RF-IgE probe unit 2434.
Described myocardial infarction analysis platform 244 includes hs-CRP (hs-CRP) probe unit 2441 and creatine kinase isozyme (CK-MB) probe unit 2442.Described hs-CRP probe unit 2441 and CK-MB probe unit 2442 are respectively used to specificity and catch hs-CRP and CK-MB, and hs-CRP and CK-MB is identified as the myocardial infarction blood serum designated object with high degree of specificity.Myocardial infarction analysis platform 244 is by the testing result of comprehensive described hs-CRP probe unit 2441 and CK-MB probe unit 2442, it is possible to myocardial infarction is quickly judged.
As shown in Figure 4, Fig. 4 is the present invention structural representation for the blood cell analysis passage preferred embodiment in the multichannel micro-fluidic chip of quick self-checking blood.In the present embodiment, described blood cell analysis passage 25 includes described platelet count platform 251, red blood cell count(RBC) platform 252, hemoglobin analysis platform 253, numeration of leukocyte platform 254 and blood type analysis platform 255.Based on the volume difference between erythrocyte, platelet and leukocyte, described platelet count platform 251, red blood cell count(RBC) platform 252 and numeration of leukocyte platform 253 sort different size of hemocyte by using different size of pipeline.
In the present embodiment, shown in Fig. 4, described platelet count platform 251 includes platelet sieve 2511 and platelet counter 2512.Described red blood cell count(RBC) platform 252 includes erythrocyte sieve 2521, erythrocyte drainage tube 2522 and erythrocytometer 2523.Described platelet sieve 2511 includes micro-parallelpiped (such as diameter 5 μm), and described platelet counter 2512 is arranged on the lower section of described platelet count platform 251.Described erythrocyte sieve 2521 includes parllel screening pipeline (such as diameter 10 μm), the bottom of described erythrocyte sieve 2521 is provided with described erythrocytometer 2522, the sidepiece of described erythrocyte sieve 2521 is provided with described erythrocyte drainage tube 2523, described erythrocyte drainage tube 2523 is connected with hemoglobinometry platform 253, and described blood cell analysis passage 25 is connected with numeration of leukocyte platform 254.
When hemocyte is directed through described platelet count platform 251 by described blood cell analysis passage 25, the minimum platelet of volume enters described platelet sieve 2511 and sinks to the bottom of described platelet sieve 2511, the platelet that screening obtains is measured based on theory of electrical impedance analysis by described platelet counter 2512, remaining hemocyte continues through described red blood cell count(RBC) platform 252, wherein the erythrocyte of small volume is sieved 2521 by described erythrocyte and screens and flow into described erythrocyte drainage tube 2523, and described erythrocyte drainage tube 2523 is intended for erythrocyte queuing to be passed through.Described erythrocytometer 2522 erythrocyte by time use, but be not limited only to, laser counting principle statistics erythrocyte number.Described erythrocyte enters described hemoglobinometry platform 253.
As it is shown in figure 5, Fig. 5 is the present invention structural representation for the hemoglobinometry platform of the multichannel micro-fluidic chip of quick self-checking blood.In the present embodiment, described hemoglobinometry platform 253 includes erythrocyte splitting unit 2531, hemoglobin test cell 2532 and valve 2533.Described erythrocyte splitting unit 2531 and described hemoglobin test cell 2532 are disc structure and are arranged in a mutually vertical manner, and are of value to solution mixing.Described valve 2533 is arranged between described erythrocyte splitting unit 2531 and hemoglobin test cell 253, has hemolytic agent in described erythrocyte splitting unit 2531.When erythrocyte enters described hemoglobin test cell 2532, described valve 2533 is opened, and described hemolytic agent flows into described hemoglobin test cell 2532, makes erythrocyte discharge hemoglobin, and described hemoglobin is combined formation haemoglobin dervative with hemolytic agent.The described hemoglobin test cell 2532 concentration by haemoglobin dervative described in absorbance measurement, the waste liquid after test is discharged by described waste fluid channel 26.
Shown in Fig. 4, staying the leukocyte in described blood cell analysis passage 25 and continue through described numeration of leukocyte platform 254, described numeration of leukocyte platform 254 is microtube structure (such as diameter 21 μm), is intended for mononuclear leukocyte queuing and passes through.Described numeration of leukocyte platform 254 rear portion is provided with leukocytometer 2541, for adding up the quantity of leucocyte by numeration of leukocyte platform 254 based on laser counting principle.Described leukocyte flows through described numeration of leukocyte platform 254 and enters described blood type analysis platform 255.Described blood type analysis platform 255 includes lysis unit 2551 and blood type analysis unit 2552, is provided with write cell lysis buffer in described lysis unit 2551.When described leukocyte flows into described lysis unit 2551, described write cell lysis buffer and described leukocyte are sufficiently mixed, leukocyte breaks formation mixed liquor, described mixed liquor flows into described blood type analysis unit 2552, described blood type analysis unit 2552 utilizes, but being not limited only to, round pcr analyzes the genotype of abo blood group, the waste liquid after analysis is discharged through described waste fluid channel 26.
The repeatable utilization of multichannel micro-fluidic chip for quick self-checking blood of the present invention, after blood testing completes, described sample intake passage 21 connects each pipeline of the inside that water pump rinses, and discharged by described waste fluid channel 26, the reagent such as described hemolytic agent and write cell lysis buffer are passable after making to be finished, but it is not limited only to, supplementary by injecting.Additionally, each pipeline configuration and pipeline junction in described multichannel micro-fluidic chip all adopt cambered structure to design, it is simple to liquid flows.Described multichannel micro-fluidic chip realizes multiple blood testing function by the microfluidic chip structure that is integrated in centimeter scale is disposable, and volume is little is easy to carry, and facilitates family's self-inspection to use.
These are only the preferred embodiments of the present invention; not thereby the scope of the claims of the present invention is limited; every equivalent structure utilizing description of the present invention and accompanying drawing content to make or equivalent function conversion; or directly or indirectly it is used in other relevant technical fields, all in like manner include in the scope of patent protection of the present invention.
Claims (9)
1. the multichannel micro-fluidic chip for quick self-checking blood, including substrate and cover plate, it is characterized in that, described cover plate is integrated with sample intake passage, inertial separation passage, split channel, plasma analysis passage and blood cell analysis passage, described sample intake passage, inertial separation passage and split channel are sequentially connected, described split channel adopts y-type structure to be connected to described plasma analysis passage and described blood cell analysis passage, wherein:
Described sample intake passage is used for inputting blood to be checked；
Described blood separation is blood plasma and hemocyte by described inertial separation passage, and described blood plasma and hemocyte flow into described split channel；
Blood plasma is carried out detection by described y-type structure and analyzes by the described blood plasma described plasma analysis passage of introducing by described split channel, and hemocyte is carried out detection analysis by the described hemocyte described blood cell analysis passage of introducing.
2. the multichannel micro-fluidic chip for quick self-checking blood according to claim 1, it is characterized in that, described cover plate is also integrated with waste fluid channel, described plasma analysis passage is all connected with described waste fluid channel with blood cell analysis passage, and described waste fluid channel is for deriving the waste liquid that the blood plasma analyzed and hemocyte produce.
3. the multichannel micro-fluidic chip for quick self-checking blood according to claim 1, it is characterised in that described plasma analysis passage includes hepatitis A analysis platform, hepatitis B analysis platform, arthritis analysis platform and myocardial infarction analysis platform.
4. the multichannel micro-fluidic chip for quick self-checking blood according to claim 3, it is characterised in that:
Described hepatitis A analysis platform includes IgM probe unit and IgA probe unit；
Described hepatitis B analysis platform includes HBsAg probe unit, HBsAb probe unit, HBeAg probe unit, HBeAb probe unit and HBcAb probe unit；
Described arthritis analysis platform includes RF-IgM probe unit, RF-IgA probe unit, RF-IgG probe unit and RF-IgE probe unit；
Described myocardial infarction analysis platform includes hs-CRP probe unit and CK-MB probe unit；
Described immunoassay platform is by comprehensively its each internal probe unit testing result detecting data judging blood plasma to blood plasma.
5. the multichannel micro-fluidic chip for quick self-checking blood according to claim 1, it is characterized in that, described blood cell analysis passage includes platelet count platform, red blood cell count(RBC) platform, hemoglobin analysis platform, numeration of leukocyte platform and blood type analysis platform.
6. the multichannel micro-fluidic chip for quick self-checking blood according to claim 5, it is characterised in that:
Described platelet count platform includes platelet sieve and platelet counter；
Described red blood cell count(RBC) platform includes erythrocyte sieve, erythrocyte drainage tube and erythrocytometer；
Described platelet count platform isolates the platelet in hemocyte by controlling the line size of described platelet sieve, and is counted by described platelet counter；
Described red blood cell count(RBC) platform isolates the erythrocyte in hemocyte by controlling the line size of described erythrocyte sieve, and is counted by described erythrocytometer；
Described numeration of leukocyte platform is isolated the leukocyte in hemocyte by controlling the line size of self and counts.
7. the multichannel micro-fluidic chip for quick self-checking blood according to claim 6, it is characterized in that, when hemocyte flows through described blood cell analysis passage, platelet is trapped in described platelet count platform, erythrocyte is through described red blood cell count(RBC) platform, entered described hemoglobin analysis platform by described erythrocyte drainage tube and carry out hemoglobin separation determination, remaining leukocyte is counted by described numeration of leukocyte platform, and enters described blood type analysis platform and carry out blood group gene type analysis.
8. the multichannel micro-fluidic chip for quick self-checking blood according to claim 5, it is characterised in that described hemoglobin analysis platform includes erythrocyte splitting unit, hemoglobin test cell and valve, wherein:
Described erythrocyte splitting unit and described hemoglobin test cell are disc structure, and are arranged in a mutually vertical manner；
Described valve is arranged between described erythrocyte splitting unit and described hemoglobin test cell；
Described erythrocyte splitting unit is provided with hemolytic agent.
9. the multichannel micro-fluidic chip for quick self-checking blood according to any one of claim 1 to 8, it is characterized in that, described sample intake passage, inertial separation passage, split channel, plasma analysis passage, blood cell analysis passage and waste fluid channel all adopt cambered structure.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610068754.XA CN105728069B (en) | 2016-01-30 | 2016-01-30 | Multi-channel micro-fluidic chip for rapidly self-checking blood |
| PCT/CN2016/101868 WO2017128768A1 (en) | 2016-01-30 | 2016-10-12 | Multi-channel microfluidic chip for fast self-testing of blood |
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| CN201610068754.XA CN105728069B (en) | 2016-01-30 | 2016-01-30 | Multi-channel micro-fluidic chip for rapidly self-checking blood |
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| CN105728069B CN105728069B (en) | 2021-01-19 |
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| CN106404640A (en) * | 2016-10-28 | 2017-02-15 | 徐廷宽 | System for detecting and analyzing blood cells |
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| CN106483182A (en) * | 2016-08-22 | 2017-03-08 | 浙江大学 | A kind of dry type determines biology sensor of hemoglobin and preparation method thereof |
| WO2017128768A1 (en) * | 2016-01-30 | 2017-08-03 | 深圳市贝沃德克生物技术研究院有限公司 | Multi-channel microfluidic chip for fast self-testing of blood |
| CN107101932A (en) * | 2017-05-28 | 2017-08-29 | 合肥赫博医疗器械有限责任公司 | A kind of micro automatic blood cell measures device with hemoglobin |
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| CN111495446A (en) * | 2020-03-23 | 2020-08-07 | 江苏大学 | Universal food allergen detection device and method based on micro-fluidic chip |
| CN112525865A (en) * | 2020-11-17 | 2021-03-19 | 西安工业大学 | Hemolysis detection optical fiber micro-fluidic sensing system and detection method |
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| CN113116348A (en) * | 2021-03-02 | 2021-07-16 | 天津大学 | Continuous arterial blood detection device |
| CN114018835A (en) * | 2021-09-22 | 2022-02-08 | 浙江大学 | Micro whole blood pretreatment and automatic quantitative plasma distribution device and analysis method |
| CN113759129A (en) * | 2021-09-26 | 2021-12-07 | 北京倍肯恒业科技发展股份有限公司 | Rapid detection card for leucocyte-bound C-reactive protein and preparation method thereof |
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