CN104133072A - Blood analysis biochip - Google Patents
Blood analysis biochip Download PDFInfo
- Publication number
- CN104133072A CN104133072A CN201410378290.3A CN201410378290A CN104133072A CN 104133072 A CN104133072 A CN 104133072A CN 201410378290 A CN201410378290 A CN 201410378290A CN 104133072 A CN104133072 A CN 104133072A
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- chip
- slide
- blood
- shim
- rubber
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Abstract
The invention relates to a blood analysis biochip, which belongs to in vitro diagnostic reagents. A detection device is composed of a slide, a rubber separator and a metal fixing clamp, the rubber separator is arranged above the slide, both sides are respectively inserted into a groove of the metal fixing clamp to fix the the chip and the rubber separator together. The preparation method is as follows: 1, activation of a nitrocellulose membrane on slide surface; 2, treatment of corresponding positions with different detecting reagents, 3, preparation of test item chips including red blood cell (RBC) chip 1, hemoglobin (Hb) chip 2, neutrophils chip 3, eosinophils chip 4, lymphocytes chip5, monocytes chip 6, white blood cell (WBC) chip 7, platelet (PLT) chip 8, and erythrocyte sedimentation chip 9; 4, the use of the chip for detection of corresponding ingredients in trace blood; and 5, data analysis.
Description
Technical field
The invention belongs to external diagnosis reagent.
Background technology
Biochip refers generally to the microarray hybridization cake core (micro-arrays) that high density is fixed on the biological information molecule (as genetic fragment, DNA fragmentation or polypeptide, protein, glycan molecule, tissue etc.) on mutual supporting dielectric, in array, the sequence of each molecule and position are known, and are pre-set sequence dot matrix.
According to the detection mode of biomolecule, biochip can roughly be divided into two types, and the first is microarray type, and it utilizes capture probe (capturing probe) to explore and is contained in the specific biochemical substances in sample.Particularly, the material that can be used as capture probe (capturing probe) is fixed on the surface of chip, then, after reacting with the biochemical substances of needs analysis, reactionless by detecting and having analyzed, and can obtain the information of biochemical substances.The second is the biochip that utilizes microfluid, microchannel, microchamber and mixing valve are set on chip, to control microfluid, biochemical substances is fixed on after detecting unit, utilize microfluid to make to need the biochemical substances detecting to react with the biochemical substances that is fixed on detecting unit, reactionless to have detected.In the long run, and according to nearest trend toward miniaturization, this is the most active field of research.
In the diagnosis of numerous disease, blood routine examination is one of most basic laboratory examination clinically.Blood routine examination project comprises red blood cell, leucocyte, haemoglobin and platelet counts etc.Routine blood test gathers and to refer to blood or ear-lobe peripheral blood with needle point method, splashes on special calculating dial, then be placed under microscope and calculate blood cell number after dilution.Conventional sign on routine blood test laboratory test report is: RBC represents red blood cell, and WBC represents leucocyte, and Hb represents haemoglobin (hemochrome), and PLT represents blood platelet.Blood biochip of the present invention can complete a plurality of patients' blood routine examination fast simultaneously, for very large effect is played in clinician's treatment.
Summary of the invention:
The object of this invention is to provide a kind of biochip that detects human blood, each film piece can detect 16 physiochemical indices simultaneously, utilize rubber shim and slide geometrical clamp, can on a chip, there be 16 film pieces, namely can detect 16 blood samples simultaneously, easy and simple to handle, save sample and time, can automatically carry out standardization correction, and generate the chart of each autoantibody signal intensity.
The present invention is by the following technical solutions: its pick-up unit is comprised of slide, rubber shim and metal clip, and rubber shim is arranged on slide top, and insert respectively in the groove of metal clip both sides, and chip and rubber shim are fixed together.
Its preparation method is: 1, the activation of surface of glass slide nitrocellulose filter; 2, different detection agent treated for corresponding position; 3, prepare test item chip, comprise red blood cell (RBC) chip 1, haemoglobin (Hb) chip 2, neutrophil leucocyte chip 3, eosinophil chip 4, lymphocyte chip 5, monocyte chip 6, leucocyte (WBC) chip 7, blood platelet (PLT) chip 8, erythrocyte sedimentation rate chip 9; 4, utilize detection chip to detect composition corresponding in micro blood; 5, data analysis: analyze with corresponding analysis software.
The described about 1mm of rubber shim thickness, size is consistent with slide, has 16 holes on shim, and the position in hole and aperture are suitable with antibody spot sample district, and rubber shim is covered to chip surface, and antibody spot sample district is positioned at the center position in each hole.
Every slide of the present invention (25mm * 73mm) is divided into 16 film pieces, and each film piece contains the correspondence detection reagent that simultaneously detects 9 physiochemical indices.Therefore, every chip all contains 16 microarraies, and each film piece is 9 indexs simultaneously.Pick-up unit is the rubber separate spacers and two geometrical clamps that have 16 holes, rubber separate spacers and slide are in the same size, on the position in its hole and size and slide, 9 positions of detecting reagent point sample districts meet, rubber shim is covered to chip surface, detect the center position that reagent point sample district is positioned at each hole.
The present invention can be easy and simple to handle, save sample and time, can automatically carry out standardization correction, and generate the chart of counter sample signal intensity.
Accompanying drawing explanation:
Fig. 1 is the structural representation of slide in the present invention;
Fig. 2 is the structural representation of slide after processing in the present invention;
Fig. 3 is the structural representation of rubber shim in the present invention;
Fig. 4 is the structural representation of metal clip in the present invention;
Fig. 5 is the assembly structure schematic diagram of pick-up unit of the present invention.
Embodiment:
With reference to Fig. 1-5, this embodiment is by the following technical solutions: its pick-up unit is comprised of slide 1, rubber shim 2 and metal clip 3, rubber shim 2 is arranged on slide 1 top, insert respectively in the groove of metal clip 3 both sides, and slide 1 and rubber shim 2 are fixed together.
Its preparation method is: 1, the activation of surface of glass slide nitrocellulose filter; 2, different detection agent treated for corresponding position; 3, prepare test item chip, comprise red blood cell (RBC) chip 1, haemoglobin (Hb) chip 2, neutrophil leucocyte chip 3, eosinophil chip 4, lymphocyte chip 5, monocyte chip 6, leucocyte (WBC) chip 7, blood platelet (PLT) chip 8, erythrocyte sedimentation rate chip 9; 4, utilize detection chip to detect composition corresponding in micro blood; 5, data analysis: analyze with corresponding analysis software.
The present invention can be easy and simple to handle, save sample and time, can automatically carry out standardization correction, and generate the chart of counter sample signal intensity.
Claims (3)
1. a blood analysis biochip, the pick-up unit that it is characterized in that it is comprised of slide, rubber shim and metal clip, rubber shim is arranged on slide top, and insert respectively in the groove of metal clip both sides, and slide and rubber shim are fixed together.
2. a kind of blood analysis biochip according to claim 1, is characterized in that: its preparation method is: 1, the activation of surface of glass slide nitrocellulose filter; 2, different detection agent treated for corresponding position; 3, prepare test item chip, comprise red blood cell (RBC) chip 1, haemoglobin (Hb) chip 2, neutrophil leucocyte chip 3, eosinophil chip 4, lymphocyte chip 5, monocyte chip 6, leucocyte (WBC) chip 7, blood platelet (PLT) chip 8, erythrocyte sedimentation rate chip 9; 4, utilize detection chip to detect composition corresponding in micro blood; 5, data analysis: analyze with corresponding analysis software.
3. a kind of blood analysis biochip according to claim 1, it is characterized in that the described about 1mm of rubber shim thickness, size is consistent with slide, on shim, there are 16 holes, the position in hole and aperture are suitable with detection reagent point sample district, rubber shim is covered to chip surface, detect the center position that reagent point sample district is positioned at each hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410378290.3A CN104133072A (en) | 2014-07-28 | 2014-07-28 | Blood analysis biochip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410378290.3A CN104133072A (en) | 2014-07-28 | 2014-07-28 | Blood analysis biochip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104133072A true CN104133072A (en) | 2014-11-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410378290.3A Pending CN104133072A (en) | 2014-07-28 | 2014-07-28 | Blood analysis biochip |
Country Status (1)
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| CN (1) | CN104133072A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105728069A (en) * | 2016-01-30 | 2016-07-06 | 深圳市贝沃德克生物技术研究院有限公司 | Multi-channel micro-fluidic chip for rapid blood self-inspection |
Citations (4)
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| CN1591012A (en) * | 2003-08-27 | 2005-03-09 | 松下电器产业株式会社 | Microchip, process of manufacturing the same, and analytical method using the same |
| CN2833593Y (en) * | 2005-10-21 | 2006-11-01 | 天津医科大学 | Cell chip detector |
| US20080293129A1 (en) * | 2007-05-23 | 2008-11-27 | Enplas Corporation | Fluid Handling Unit and Fluid Handling Apparatus Using Same |
| US20110241134A1 (en) * | 2010-03-31 | 2011-10-06 | Koichi Ono | Micro-channel chip and micro-analysis system |
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2014
- 2014-07-28 CN CN201410378290.3A patent/CN104133072A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1591012A (en) * | 2003-08-27 | 2005-03-09 | 松下电器产业株式会社 | Microchip, process of manufacturing the same, and analytical method using the same |
| CN2833593Y (en) * | 2005-10-21 | 2006-11-01 | 天津医科大学 | Cell chip detector |
| US20080293129A1 (en) * | 2007-05-23 | 2008-11-27 | Enplas Corporation | Fluid Handling Unit and Fluid Handling Apparatus Using Same |
| US20110241134A1 (en) * | 2010-03-31 | 2011-10-06 | Koichi Ono | Micro-channel chip and micro-analysis system |
Non-Patent Citations (1)
| Title |
|---|
| 张鹏等: "一种基于硝酸纤维素膜的新颖便捷的芯片对循环肿瘤细胞的高效捕获", 《色谱》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105728069A (en) * | 2016-01-30 | 2016-07-06 | 深圳市贝沃德克生物技术研究院有限公司 | Multi-channel micro-fluidic chip for rapid blood self-inspection |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141105 |