CN206594187U - Magnetic analytes collect transfer system - Google Patents
Magnetic analytes collect transfer system Download PDFInfo
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- CN206594187U CN206594187U CN201720246752.5U CN201720246752U CN206594187U CN 206594187 U CN206594187 U CN 206594187U CN 201720246752 U CN201720246752 U CN 201720246752U CN 206594187 U CN206594187 U CN 206594187U
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- magnetic
- magnetosheath
- analytes
- transfer system
- magnet
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Abstract
Transfer system is collected the utility model discloses a kind of magnetic analytes, including collection portion, elution portion, the collection portion includes the container that bar magnet and size are suitable for place into bar magnet, and the bar magnet includes magnetic core and magnetosheath, and being dimensioned for of the magnetosheath is removably placed on outside the magnetic core；The elution portion is provided with magnet, for attracting magnetic analytes to be come off from magnetosheath.The utility model is related to analyte or the fully-automated synthesis apparatus field of extract, because the bar magnet in the system has magnetosheath structure, it can be engaged with the magnet in elution portion, prevent the problem of general electromagnet or permanent magnet are difficult to depart from after the completion of adsorbing, the error in reading produced during CTC Nasopharyngeal neoplasms is reduced, while avoiding the pollution to magnetic core such as cushioning liquid, antibody magnetic bead.
Description
Technical field
The utility model is related to analyte or the fully-automated synthesis apparatus field of extract, specifically provides a kind of be used for pair
System and its application method that magnetic analytes are collected and shifted.
Background technology
Circulating tumor cell (Circulating tumor cell, CTC) refers to cyclic system is come off and entered from tumour
The cancer cell of system, it can be spontaneous or come off by operation of diagnosis and treatment from entity tumor focus (primary tumor or transfer stove), is followed into body
Loop system (such as peripheral blood).Most of CTC can occur apoptosis or swallowed after entering peripheral blood, and minority can escape and anchor disease
On tissue or organ beyond stove, and then develop into transfer stove, increase malignant tumor patient mortality risk.These cells can be with
Found by simple blood testing, can as cancer biological markers, whether there is tumour, and it in indication body
Hereditary conditions and drug susceptibility.Different from surgical intervention, CTC is compatible with longitudinal research, being capable of effective guiding treatment.
CTC detections can be effectively applied to external early diagnosis, prognostic evaluation, (including rapid clinical chemotherapeutics is sieved individualized treatment
Choosing and the detection of drug resistance), tumor recurrence monitoring and the exploitation of new tumour medicine etc..But CTC detecting steps are more, it is numerous to operate
It is trivial, it also counteracts that the popularization of this technology clinically.
Conventional CTC detections at present are divided into two steps, i.e. CTC enrichments (or capture) step and CTC detection and analysis steps.
Conventional CTC capture techniques have gradient density centrifugal technology, filter film technology, Flow Cytometry, microfluidic chip technology and exempted from
Epidemic disease magnetic bead technology.Wherein, immunomagnetic bead technique is related to the steps such as the mixing of sample, cell magnetic suck, is adapted in large volume loading
Under the conditions of a small amount of special cells are separated, for CTC detection application it is especially advantageous, its operating procedure is relatively easy, easily
Automated in realizing.
Based on immunological magnetic bead sorting primary reason, need to swell with CTC in completion antibody magnetic bead using the CTC detecting systems of the principle
After the hatching combination step of oncocyte, it will combine and be transferred to after completing antibody enrichment with magnetic bead in reading platform or reading box, so that
Complete the statistics to circulating tumor cell quantity.The circulating tumor cell enrichment plant that the country has been listed at present is Johson ＆ Johnson
Cellsearch systems and ctcbiopsy systems (non-magnetic capture systems employ the mode of physical filtering), wherein
Cellsearch systems capture circulating tumor cell using magnetic frame form, and transfer step needs to be moved after magnet demagnetization
Take and shift, easily causing liquid residue or absorption causes the loss of circulating tumor cell.In addition, magnetic core is generally with delaying during elution
Fliud flushing is directly contacted, and solution is polluted.
Therefore, those skilled in the art is directed to developing a kind of magnetic analytes collection transfer system, is easy to combine
Frame of reference quantity performed statistics is transferred to after the antibody enrichment with magnetic bead for having circulating tumor cell, and requires that transfer efficiency is high, nothing
Pollution.
Utility model content
In view of the drawbacks described above of prior art, technical problem to be solved in the utility model is to provide a kind of magnetic point
Analyse thing and collect transfer system, including collection portion, elution portion, the collection portion includes bar magnet and size is suitable for place into the appearance of bar magnet
Device, the bar magnet includes magnetic core and magnetosheath, and being dimensioned for of the magnetosheath is removably placed on outside the magnetic core；The elution
Portion is provided with magnet, for attracting magnetic analytes to be come off from magnetosheath.
Further, the magnetic core is cylindrical magnet.
Further, the thickness of the magnetosheath is 0.5-3mm.
Further, the elution portion is provided with observation window, directly to be entered by observation window to the magnetic analytes of elution
Row observation.
Further, the elution portion is additionally provided with vibrating motor, is come off for assisted magnetic analyte from magnetosheath.
Further, screen magnetic epitheca is also wrapped on outside the magnetic core, only exposes the end face at a magnetic pole, the screen magnetic
Epitheca is made by soft magnetic materials.
Further, the magnetic analytes are the antibody magnetic bead for being combined with circulating tumor cell.
Technique effect
Because the bar magnet in the system has magnetosheath structure, it can be engaged with the magnet in elution portion, it is therefore prevented that ordinary electromagnetic
The problem of being difficult to depart from after the completion of iron or permanent magnet absorption, the error in reading produced during CTC Nasopharyngeal neoplasms is reduced, together
When avoid the pollution to magnetic core such as cushioning liquid, antibody magnetic bead.
The technique effect of design of the present utility model, concrete structure and generation is made furtherly below with reference to accompanying drawing
It is bright, to be fully understood from the purpose of this utility model, feature and effect.
Brief description of the drawings
Fig. 1 is the structural representation front view of bar magnet in a preferred embodiment of the present utility model；
Fig. 2 is sectional view of the bar magnet along A-A direction structures in Fig. 1 embodiments；
Fig. 3 is the structural representation in elution portion in Fig. 1 embodiments；
Fig. 4 is the structural representation sectional view of elution portion base in Fig. 3；
Reference：
101- plastic barrier magnetosheaths, 102- magnetic cores, 103- screen magnetic epithecas, 104- inserted links, 105- righting damping sleeves；
201- reading box bases, 202- bar magnet spacing holes, 203 vibrating motors；
301- glass culture dish, 302- permanent magnets, 303- aluminium alloy magnetic supports.
Embodiment
Embodiment of the present utility model is described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning
Same or similar element or element with same or like function are represented to same or similar label eventually.Below by ginseng
The embodiment for examining accompanying drawing description is exemplary, it is intended to for explaining the utility model, and it is not intended that to the utility model
Limitation.
As shown in the sectional view Fig. 2 in front view Fig. 1 and A-A directions, the present embodiment provide firstly magnetic analytes collection
It is used for the bar magnet collected, including magnetic core 102, screen magnetic epitheca 103, plastic barrier magnetosheath 101 in transfer system.Wherein magnetic core 102
For diameter 8mm, long 33mm cylindric ndfeb magnet, outside is coated with external diameter 12mm silicon steel pipe, that is, shields magnetic epitheca 103,
Shield the lower ending opening of magnetic epitheca 103, it is concordant with magnet, expose the lower surface of magnetic core 102.The isolation of coating plastic on the outside of silicon steel pipe
With magnetosheath 101, silicon steel tube panel magnetic epitheca 103 and magnetic core 102 are wrapped in inside it.
Because silicon steel pipe has high magnetic conductivity as soft magnetic materials, therefore silicon steel pipe ought be covered in ndfeb magnet
During surrounding, the magnetic induction line of this peripheral diverging will be gathered in inside soft magnetic materials, and external magnetic field strength is almost 0, so as to play pair
The effect of the side wall magnetic screen of magnetic core 102, prevents magnetic bead absorption from influenceing to measure in magnet side wall.Permanent magnet and soft magnetic substance it is this
Combination to greatest extent can all deflect in the magnetic field of magnet script increased radioactivity to the end face exposed, while improving poly-
Ji Chu magnetic field intensity, wherein the capture plane for the enrichment of magnetic particles is the lower surface that magnetic core 102 exposes.
Plastic barrier magnetosheath 101 is placed on outside magnetic core, not only ensure that and do not contacted with magnetic core directly, it is to avoid contact is brought
Pollution, while can remove magnetic core in elution step, and attract antibody magnetic bead to leave plastic barrier magnetic by externally-applied magnetic field
Set 101.In addition, the top that magnetic core 102 and soft magnetic type screen magnetic epitheca 103 are combined is further fixed on inserted link 104, the inserted link 104 is connected
Magnetic core 102 and control unit, draw the inserted link 104 using mechanical device and slowly transport being contained with the centrifuge tube of antibody magnetic bead
It is dynamic, until attracting complete to antibody magnetic bead.
The top of plastic barrier magnetosheath 101 is additionally provided with righting damping sleeve 105.The righting damping sleeve 105 is used to facilitate inserted link
Interfixing between 104 and plastic barrier magnetosheath 101, when performing absorption and desorption manipulation in fluid sample, it usually needs
It is carried out it is in rotary moving wait operation, this part makes bar magnet turn into entirety, is easy to its motion in fluid sample, and subtract
Few energy loss brought by factors such as concussion, fluid resistances.
The present embodiment also discloses this magnetic analytes and collected in transfer system with collection system cooperation for magnetic point
Analyse the apparatus structure of thing elution.As shown in figure 3, the washing device includes multigroup elution unit, each unit includes a packet size
The reading box base 201 and bar magnet spacing hole 202 being adapted with bar magnet size.The side that base 201 is combined is additionally provided with vibration
Motor 203, vibrating motor is connected with base, and base can be driven to produce vibration, and help antibody magnetic bead is cooperateed with righting damping sleeve 105
Come off from the surface of plastic barrier magnetosheath 101.
The structure of each reading box base 201 is further as shown in figure 4, the housing of this base 201 is aluminium alloy magnetic support 303.
Glass culture dish 301 is provided with magnetic support 303 as container, PBS cushioning liquid can be held, while being put into bar magnet.Culture dish bottom
Portion is transparent area, and the setting of transparent area causes experimenter the base 201 for completing capture directly can be transferred into light microscope and entered
Row observation is counted.In order to realize the target for attracting auxiliary antibody magnetic bead to come off by magnet, this base 201 is parallel same using two pieces
High-intensity magnetic field is provided to the permanent magnet 302 of setting, two pieces of permanent magnets 302 are respectively arranged at the two ends of transparent area, flat perpendicular to printing opacity
Face is set in the same direction, and around glass culture dish 301.Using the parallel extruding of two pieces of own magnetic force lines of permanent magnet 302, saturating
Magnetic field of the direction perpendicular to the bottom of glass culture dish 301 is formed in light area.When magnetic analytes to be tested enter the magnetic field
When, the circulating tumor cell for being combined with magnetic bead being incubated will be pressed in the base plane of transparent utensil, so as to provide
The fixation CTC viewing planes of one plane tiling.
The collection that the present embodiment is provided has collectively constituted this collection transfer system with bar magnet and washing device, because bar magnet is adopted
, can be by magnetic core 102 from isolation magnetosheath 101 with plastic barrier magnetosheath 101, the demountable structure of magnetic core 102, therefore at washing device
It is middle to take out, the external magnetic field near magnetosheath 101 is disappeared.Now, partial antibody magnetic bead due to Action of Gravity Field can be directly off magnetosheath
101 surfaces, partial antibody magnetic bead due to left magnetic effect or adhesion effect will still rest on the surface of magnetosheath 101.The present embodiment
The base device of middle offer, the externally-applied magnetic field on the one hand provided by two pieces of permanent magnets 302 of the bottom of base 201 draws antibody magnetic
Pearl, on the other hand shakes base 201 by the vibrating motor 203 of the side of base 201, helps the antibody magnetic bead that does not leave from isolation
The surface of magnetosheath 101 comes off.
Collection transfer system in the present embodiment can be used for CTC circulating tumor cell detecting systems, and circulating tumor cell is same
After antibody magnetic bead hatching combination, after the collection transfer system enrichment in the present embodiment, it is transferred to frame of reference and is read
Number.Because the magnetic core in the system, magnetosheath are disconnected from each other, using disposable cleaning magnetosheath, the pollution of magnetic core is prevented.
The concrete operation method of collection transfer system in the present embodiment is as follows：
S1：Antibody magnetic bead is incubated altogether with circulating tumor cell；
S2：Bar magnet (with magnetosheath) is slow in the system after the completion of incubation to carry out motion a cycle from top to bottom；
S3：Bar magnet (band magnetosheath) is rinsed for several times in PBS；
S4：In reading box of bar magnet (with magnetosheath) insertion containing PBS, the magnetic core for extracting bar magnet (only stays bar magnet set
In reading box), periodically reading box is shaken in left and right, collects the circulating tumor cell on magnetosheath into reading box.
Preferred embodiment of the present utility model described in detail above.It should be appreciated that the ordinary skill people of this area
Member just can make many modifications and variations without creative work according to design of the present utility model.Therefore, all this technology necks
Technical staff passes through logic analysis, reasoning or limited reality on the basis of existing technology according to design of the present utility model in domain
Available technical scheme is tested, all should be in the protection domain being defined in the patent claims.
Claims (7)
1. a kind of magnetic analytes collect transfer system, it is characterised in that including collection portion, elution portion, the collection portion includes
Bar magnet and size are suitable for place into the container of bar magnet, and the bar magnet includes magnetic core and magnetosheath, and being dimensioned for for the magnetosheath is detachable
Ground is placed on outside the magnetic core；The elution portion is provided with magnet, for attracting magnetic analytes to be come off from magnetosheath.
2. magnetic analytes as claimed in claim 1 collect transfer system, it is characterised in that the magnetic core is cylindrical magnet.
3. magnetic analytes as claimed in claim 1 collect transfer system, it is characterised in that the thickness of the magnetosheath is 0.5-
3mm。
4. magnetic analytes as claimed in claim 1 collect transfer system, it is characterised in that the elution portion is provided with observation
Window, directly to be observed by observation window the magnetic analytes of elution.
5. magnetic analytes as claimed in claim 1 collect transfer system, it is characterised in that the elution portion, which is additionally provided with, to shake
Dynamic motor, comes off for assisted magnetic analyte from magnetosheath.
6. magnetic analytes as claimed in claim 1 collect transfer system, it is characterised in that be also wrapped on outside the magnetic core
Shield magnetic epitheca, only expose the end face at a magnetic pole, the screen magnetic epitheca is made by soft magnetic materials.
7. magnetic analytes as claimed in claim 1 collect transfer system, it is characterised in that the magnetic analytes are combination
There is the antibody magnetic bead of circulating tumor cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720246752.5U CN206594187U (en) | 2017-03-14 | 2017-03-14 | Magnetic analytes collect transfer system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720246752.5U CN206594187U (en) | 2017-03-14 | 2017-03-14 | Magnetic analytes collect transfer system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771307A (en) * | 2017-03-14 | 2017-05-31 | 骏实生物科技(上海)有限公司 | Magnetic analytes collect transfer system and its method |
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- 2017-03-14 CN CN201720246752.5U patent/CN206594187U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771307A (en) * | 2017-03-14 | 2017-05-31 | 骏实生物科技(上海)有限公司 | Magnetic analytes collect transfer system and its method |
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