CN109781999A - A kind of magnetic immunochemiluminescence detection method of PD-L1 excretion body - Google Patents
A kind of magnetic immunochemiluminescence detection method of PD-L1 excretion body Download PDFInfo
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- CN109781999A CN109781999A CN201910145178.8A CN201910145178A CN109781999A CN 109781999 A CN109781999 A CN 109781999A CN 201910145178 A CN201910145178 A CN 201910145178A CN 109781999 A CN109781999 A CN 109781999A
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Abstract
The invention discloses a kind of magnetic immunochemiluminescence detection methods of PD-L1 excretion body, belong to external rapid detection technical field.Detection used kit contains the hollow posts and magnet that antibody A, antibody B, porous foam metal are filled, and the antibody A is the excretion body antibody of chemiluminescent molecule or the enzyme label that substrate can be made luminous, and the antibody B is the excretion body antibody of marked by magnetic bead；At least a kind of in the antibody A and antibody B is excretion body PD-L1 antibody.Detection method is then to realize efficient capture separation by porous foam metal magnetic first by antibody A, antibody B and sample incubation, after cleaning impurity, removes magnetic field, collects double antibodies sandwich compound and detect.The method integrates sample incubation, and the separation of magnetic efficient capture and quickly detection have the features such as easy to operate, detection is quick and at low cost.
Description
Technical field
The invention belongs to external rapid detection technical fields, and in particular to a kind of magnetic immunochemistry hair of PD-L1 excretion body
Light detection kit and detection method.
Background technique
Excretion body is the nanoscale vesicles (diameter 50-150nm) that cell is secreted into extracellular microenvironment, for the first time 1983
It is found.But due to the shortage of analysis means, excretion body is considered as the tool that cell drains rubbish intracellular for a long time.With
Analysis method is constantly brought forth new ideas, and the mechanism of excretion body secretion is gradually revealed.Now, the viewpoint of scientific circles' mainstream thinks, excretion
Body is primarily involved in intercellular signal transmitting and as disease markers.Nobel Prize in Physiology or Medicine is granted by announcement within 2013
Three distinguished scientists of the great discovery in this field.However, the excretion body function research thus caused and the following potential application
Unlimited reverie is brought to international academic community.2015, by bioluminescence imaging technology, the Vesicles such as excretion body passed extensively in vivo
Passing is confirmed；And more breathtaking discovery, excretion body are just overturning cognition of the scientific circles to cancer development: traditional view is recognized
For metastases are directly to settle its hetero-organization by blood circulation system due to primary tumor tumour cell.Which results in when
Lower circulating tumor cell research has an unprecedently grand occasion；Under normal circumstances, T cell cracks cancer cell automatically after differentiating cancer cell,
Maintain the balance of human body.But cancer cell surfaces express the special protein (receptor) of one kind and T cell combines, and T cell is enabled to produce
Raw " illusion ", it is believed that cancer cell is " harmless ", while the activity of T cell is reduced, and cancer cell is saved oneself.It is this special
Protein be exactly PD-L1 (ligand of programmed death receptor -1).But newest excretion body analysis is found, tumour is to divide first
Excretion body is secreted as vanguard, its hetero-organization is transmitted to by blood circulation system, and microenvironment is transformed to adapt it to tumour thin
After intracellular growth, then diverts oneself from loneliness or boredom tumour cell main forces and camp.This discovery refreshes again or has overturned our understanding to cancer,
It is confirmed finally about " seed " of cancer and " soil " hypothesis over 100 years.Exciting, nearest associate professor Chen Gang exists
It finds that the excretion body that tumour cell can be rich in PD-L1 by release surface plays the function to tumoricidal T cell in studying
Can inhibiting effect, and in peripheral blood the concentration level of PD-L1 positive excretion body and tumor size in be positively correlated closely (Nature,
2018,560(7718):382-386)。
Isolating and purifying for excretion body mainly is carried out by supercentrifugation to the detection of excretion body in body fluid at present, it is time-consuming
Effort is unfavorable for clinical quickly detection.The prior art is mainly to concentrate the four cross-film superfamily eggs in analysis excretion body surface face simultaneously
The excretion body surface face of white (CD63/CD9/CD81 etc.), normal cell secretion also carry four cross-film superfamily proteins, so big at present
Most excretion body testing goals are to be detected based on excretion body abundance, and do not have specificity.
Magnetic immunochemistry analysis platform method at present, entire reaction system concentrate in centrifuge tube, exist be incubated for it is insufficient,
The disadvantages of undercompounding and Magneto separate low efficiency.
Summary of the invention
The present invention solves not high, the inconvenient and at high cost technology of the survey specificity of excretion physical examination in the prior art and asks
The technical issues of low separation efficiency is immunized in topic and magnetic.
It is according to the invention in a first aspect, provide a kind of magnetic immunochemiluminescence detection kit of PD-L1 excretion body,
The kit contains the hollow posts and magnet that antibody A, antibody B, porous foam metal are filled, and the antibody A is chemiluminescence
The excretion body antibody of molecule or the enzyme label that substrate can be made luminous, the antibody B are the excretion body antibody of marked by magnetic bead；It is described anti-
At least a kind of in body A and antibody B is excretion body PD-L1 antibody.
Preferably, the porous foam metal is porous foam iron, porous foam cobalt or porous foam nickel；
Preferably, the diameter of the hole of the porous foam metal is 10um-100um.
Preferably, the chemiluminescent molecule is acridine rouge；The enzyme horseradish peroxidase that substrate can be made luminous.
Preferably, the magnetic bead is paramagnetic beads, diameter 10nm-1000nm.
It is another aspect of this invention to provide that providing chemiluminescent molecule or the excretion body of enzyme label that substrate can be made luminous
The excretion body antibody of antibody and marked by magnetic bead is used to prepare the application in detection PD-L1 excretion body reagent, contains following steps:
(1) it sample incubation: after sample to be tested and antibody A and antibody B are sufficiently mixed, is incubated for obtain target compound
Object；The antibody A is the excretion body antibody of chemiluminescent molecule or the enzyme label that substrate can be made luminous, and the antibody B is magnetic bead
The excretion body antibody of label；At least a kind of in the antibody A and antibody B is excretion body PD-L1 antibody；The target complex
The double-antibody sandwich compound formed for antibody A, PD-L1 excretion body and antibody B；
(2) magnetic separates: magnet being adsorbed onto the hollow pipe outer wall of porous foam metal filling, makes the porous foam gold
Category is placed in magnetic field environment；The hollow tube that the Incubating Solution that step (1) obtains is filled by porous foam metal again makes described double
Antibody sandwich compound is adsorbed on porous foam metal；
(3) double-antibody sandwich compound is eluted: with the inner cavity for the hollow tube that buffer solution for cleaning porous foam metal is filled；Again
The magnet of the hollow pipe outer wall of porous foam metal filling is removed, and affords double-antibody sandwich compound；
(4) luminous intensity: the luminous intensity or dual anti-for the double-antibody sandwich compound that detecting step (3) elution obtains is detected
The intensity that body sandwich complex keeps substrate luminous calculates the concentration of excretion body PD-L1 antibody in sample to be tested.
Preferably, the porous foam metal is porous foam iron, porous foam cobalt or porous foam nickel；
Preferably, the diameter of the porous foam metal Hole is 10um-100um.
Preferably, the chemiluminescent molecule is acridine rouge, the enzyme horseradish peroxidase that substrate can be made luminous.
Preferably, the magnetic bead is paramagnetic beads, diameter 10nm-1000nm.
Preferably, liquid absorption device or liquid-transfering gun are utilized in the step (2), the resorption that gets off above takes mode, by step (1)
The hollow tube that obtained Incubating Solution is filled by porous foam metal makes the double-antibody sandwich compound be adsorbed in porous foam
On metal.
Preferably, liquid absorption device or liquid-transfering gun are utilized in the step (3), the resorption that gets off above takes mode, cleans porous
The inner cavity of foam metal filled hollow tube.
In general, through the invention it is contemplated above technical scheme is compared with the prior art, mainly have below
Technological merit:
(1) detection method in the present invention integrates sample incubation, and the separation of magnetic efficient capture and quickly detection have spy
It is anisotropic strong, it is easy to operate, detect the features such as quick and at low cost.The present invention is using on chemiluminescent molecule labelled antibody
At least a kind of in antibody on antibody and marked by magnetic bead antibody is excretion body PD-L1 antibody, chemiluminescent molecule labelled antibody
On antibody and marked by magnetic bead antibody on antibody can be selected from excretion body CD63 antibody, excretion body CD81 antibody, excretion body
CD9 antibody and excretion body PD-L1 antibody.Form the double of magnetic bead antibody-excretion body PD-L1- chemiluminescent molecule label antibody
The target complex of antibody sandwich, specificity is high, and by the target complex efficient capture in porous foam metal surface.It is porous
On the one hand foam metal is able to achieve local magnetic field amplification, still further aspect also can increase incubation mixed effect, increase touching for molecule
Probability is hit, Magneto separate is high-efficient.
(2) present invention preferably uses liquid absorption devices or liquid-transfering gun by cleaning solution, and the resorption that gets off above takes mode, makes to be incubated for
The hollow tube of liquid multipass porous foam metal filling, makes the double-antibody sandwich compound more fully be adsorbed in porous bubble
On foam metal；Present invention preferably uses liquid absorption devices or liquid-transfering gun by cleaning solution, and the resorption that gets off above takes mode, realizes impurity
Effective cleaning.
(3) present invention preferably selects multiple strip magnets to be arranged in hollow pipe outer wall in a symmetrical, can also use
Hollow tube is placed in one by single circular magnet, provides stable magnetic field to porous foam metal convenient for absorption double-antibody sandwich
Target complex.
Detailed description of the invention
Fig. 1 is the flow chart of the magnetic immunochemiluminescence detection method for the PD-L1 excretion body that the embodiment of the present invention 1 provides.
Fig. 2 is the flow chart of the magnetic immunochemiluminescence detection method for the PD-L1 excretion body that the embodiment of the present invention 7 provides.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below
Not constituting a conflict with each other can be combined with each other.
The preferred embodiment that the invention will now be described in detail with reference to the accompanying drawings.
Embodiment 1
A kind of magnetic immunochemiluminescence detection kit of PD-L1 excretion body, the excretion containing chemiluminescent molecule label
The hollow posts and magnet that body antibody, the excretion body antibody of paramagnetic beads label, porous foam metal are filled；The chemiluminescence point
Antibody on sub- labelled antibody is excretion body PD-L1 antibody, and the antibody on marked by magnetic bead antibody is excretion body PD-L1 antibody.Institute
Stating porous foam metal is porous foam iron.The diameter of the porous foam iron Hole is 10um.The chemiluminescent molecule
For horseradish peroxidase.The diameter of the paramagnetic beads is 10nm.
Embodiment 2
A kind of magnetic immunochemiluminescence detection kit of PD-L1 excretion body, the excretion containing chemiluminescent molecule label
The hollow posts and magnet that body antibody, the excretion body antibody of paramagnetic beads label, porous foam metal are filled；The chemiluminescence point
Antibody on sub- labelled antibody is excretion body PD-L1 antibody, and the antibody on marked by magnetic bead antibody is excretion body CD63 antibody.It is described
Porous foam metal is porous foam nickel.The diameter of the porous foam nickel Hole is 50um.The chemiluminescent molecule is
Acridine rouge.The diameter of the paramagnetic beads is 50nm.
Embodiment 3
A kind of magnetic immunochemiluminescence detection kit of PD-L1 excretion body, the excretion containing chemiluminescent molecule label
The hollow posts and magnet that body antibody, the excretion body antibody of paramagnetic beads label, porous foam metal are filled；The chemiluminescence point
Antibody on sub- labelled antibody is excretion body CD63 antibody, and the antibody on marked by magnetic bead antibody is excretion body PD-L1 antibody.It is described
Porous foam metal is porous foam cobalt.The diameter of the porous foam cobalt Hole is 100um.The chemiluminescent molecule is
Horseradish peroxidase.The diameter of the paramagnetic beads is 1000nm.
Embodiment 4
Chemiluminescent molecule in this embodiment selects horseradish peroxidase, and luminescence system uses luminol chemiluminescence
System；
(1) it sample incubation: indicates the antibody of horseradish peroxidase, indicate paramagnetic nanoparticles magnetic bead antibody and sample is incubated
It educates, incubation time is 30 minutes.The antibody indicated on the antibody of horseradish peroxidase is excretion body PD-L1 antibody, is indicated suitable
Antibody on the antibody of magnetic Nano magnetic bead is excretion body PD-L1 antibody.The diameter of paramagnetic nanoparticles magnetic bead is 50nm.
(2) magnetic separates: in the case where reinforcing magnetic field outside, amplifying by porous foam nickel to local magnetic field, realizes high
Effect capture separation.Porous foam metal is filled in pipette tips or in the pipe of analogous shape, will using liquid absorption device or liquid-transfering gun
Above-mentioned mixtures incubated altogether, the above-resorption that gets off takes mode, by target complex (magnetic bead antibody-excretion body-horseradish peroxidating
The antibody of object enzyme) efficient capture is in porous foam nickel surface.2 strip magnets are arranged in hollow pipe outer wall in a symmetrical, give
Porous foam metal provides stable magnetic field convenient for the target complex of absorption double-antibody sandwich.The diameter of porous foam nickel is
10um.On the one hand porous foam nickel is able to achieve local magnetic field amplification, still further aspect also can increase mixed effect, and it is several to increase collision
Rate.
(3) it elutes: cleaning solution cleaning is added, removes non-specific target, using liquid absorption device or liquid-transfering gun by cleaning solution,
Above-the resorption that gets off takes mode, realizes effective cleaning purpose.
(4) release detection: removing externally-applied magnetic field, elutes target complex, the target complex detected.Using shine
Substrate solution (luminol+hydrogen peroxide series) more than-resorption that gets off takes mode, efficiently discharges target complex.Utilize chemistry
Luminometer device detects light emission luminance, calculates excretion body PD-L1 excretion bulk concentration in sample.
Embodiment 5
Chemiluminescent molecule in this embodiment selects acridine rouge, and luminescence system is using hydrogen peroxide+sodium hydroxide chemistry
Luminescence system；
(1) sample incubation: indicating the antibody of acridine rouge, indicate paramagnetic nanoparticles magnetic bead antibody and sample incubation, when incubation
Between be 40 minutes.The antibody on the antibody of acridine rouge is indicated for excretion body PD-L1, on the antibody for indicating paramagnetic nanoparticles magnetic bead
Antibody is excretion body CD63 antibody.The diameter of paramagnetic nanoparticles magnetic bead is 1000nm.
(2) magnetic separates: in the case where reinforcing magnetic field outside, amplifying by porous foam cobalt to local magnetic field, realizes high
Effect capture separation.Porous foam metal is filled in the pipe of similar pipette tips shape, will be above-mentioned using liquid absorption device or liquid-transfering gun
Mixtures incubated, the above-resorption that gets off take mode altogether, by target complex (magnetic bead antibody-excretion body-acridine rouge antibody) height
Effect is trapped in porous foam nickel surface.Hollow tube is placed in one by single circular magnet, provides to porous foam metal stable
It is convenient for the target complex of absorption double-antibody sandwich in magnetic field.The diameter of porous foam cobalt is 100um.Porous foam cobalt one side energy
Realize local magnetic field amplification, still further aspect also can increase mixed effect, increase collision probability.
(3) it elutes: cleaning solution cleaning is added, removes non-specific target, using liquid absorption device or liquid-transfering gun by cleaning solution,
Above-the resorption that gets off takes mode, realizes effective cleaning purpose.
(4) release detection: removing externally-applied magnetic field, elutes target complex, the target complex detected.Using shine
Substrate solution (hydrogen peroxide+sodium hydroxide series) more than-resorption that gets off takes mode, efficiently discharges target complex.Utilize change
It learns luminometer device and detects light emission luminance, calculate excretion body PD-L1 excretion bulk concentration in sample.
Embodiment 6
Chemiluminescent molecule in this embodiment selects horseradish peroxidase, and luminescence system uses luminol chemiluminescence
System；
(1) it sample incubation: indicates the antibody of horseradish peroxidase, indicate paramagnetic nanoparticles magnetic bead antibody and sample is incubated
It educates, incubation time is 50 minutes.The antibody indicated on the antibody of horseradish peroxidase is excretion body CD63 antibody, indicates paramagnetic
Property nanometer magnetic bead antibody on antibody be excretion body PD-L1 antibody.The diameter of paramagnetic nanoparticles magnetic bead is 10nm.
(2) magnetic separates: in the case where reinforcing magnetic field outside, amplifying by porous foam iron to local magnetic field, realizes high
Effect capture separation, using liquid absorption device or liquid-transfering gun by above-mentioned mixtures incubated altogether, the above-resorption that gets off takes mode, by target
Compound (magnetic bead antibody-excretion body-horseradish peroxidase antibody) efficient capture is in porous foam iron surface.Porous foam
The diameter of iron is 50um.On the one hand porous foam iron is able to achieve local magnetic field amplification, still further aspect also can increase mixed effect,
Increase collision probability.
(3) it elutes: cleaning solution cleaning is added, removes non-specific target, using liquid absorption device or liquid-transfering gun by cleaning solution,
Above-the resorption that gets off takes mode, realizes effective cleaning purpose.
(4) release detection: removing externally-applied magnetic field, elutes target complex, the target complex detected.Using shine
Substrate solution (luminol+hydrogen peroxide series) more than-resorption that gets off takes mode, efficiently discharges target complex.Utilize chemistry
Luminometer device detects light emission luminance, calculates excretion body PD-L1 excretion bulk concentration in sample.
Embodiment 7
Chemiluminescent molecule in this embodiment selects acridine rouge, and preexciting liquid is hydrogen peroxide, and exciting liquid is hydroxide
Sodium；
(1) sample incubation: indicating the antibody of acridine rouge, indicate magnetic bead antibody and sample incubation, and incubation time is 30 minutes.
(2) magnetic separates: in the case where reinforcing magnetic field outside, amplifying by porous foam nickel to local magnetic field, realizes high
Effect capture separation, using liquid absorption device or liquid-transfering gun by above-mentioned mixtures incubated altogether, the above-resorption that gets off takes mode, by target
Compound (magnetic bead antibody-excretion body-acridine rouge antibody) efficient capture is in porous foam nickel surface.Porous foam nickel one side energy
Realize local magnetic field amplification, still further aspect also can increase mixed effect, increase collision probability.
(3) it elutes: cleaning solution cleaning is added, remove non-specific target.Using liquid absorption device or liquid-transfering gun by cleaning solution,
Above-the resorption that gets off takes mode, realizes effective cleaning purpose.
(4) release detection: removing externally-applied magnetic field, elutes target complex, the target complex detected.Swashed using pre-
Lotion hydrogen peroxide, the above-resorption that gets off take mode, efficiently discharge target complex.Exciting liquid sodium hydroxide is added later
It is detected, this luminescence system belongs to transient luminescence type, carries out signal detection using photomultiplier tube PMT.Utilize chemiluminescence
Detecting instrument detects light emission luminance, calculates excretion body PD-L1 excretion bulk concentration in sample.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all include
Within protection scope of the present invention.
Claims (10)
1. a kind of magnetic immunochemiluminescence detection kit of PD-L1 excretion body, which is characterized in that the kit contains antibody
A, the hollow posts and magnet that antibody B, porous foam metal are filled, the antibody A are chemiluminescent molecule or substrate can be made to shine
Enzyme label excretion body antibody, the antibody B be marked by magnetic bead excretion body antibody；In the antibody A and antibody B at least
One kind is excretion body PD-L1 antibody.
2. the magnetic immunochemiluminescence detection kit of PD-L1 excretion body as described in claim 1, which is characterized in that described
Porous foam metal is porous foam iron, porous foam cobalt or porous foam nickel；
Preferably, the diameter of the hole of the porous foam metal is 10um-100um.
3. the magnetic immunochemiluminescence detection kit of PD-L1 excretion body as described in claim 1, which is characterized in that described
Chemiluminescent molecule is acridine rouge；The enzyme horseradish peroxidase that substrate can be made luminous.
4. the magnetic immunochemiluminescence detection kit of PD-L1 excretion body as described in claim 1, which is characterized in that described
Magnetic bead is paramagnetic beads, diameter 10nm-1000nm.
5. the excretion body antibody of the excretion body antibody and marked by magnetic bead of chemiluminescent molecule or the enzyme label that substrate can be made luminous
The application being used to prepare in detection PD-L1 excretion body reagent, which is characterized in that contain following steps:
(1) it sample incubation: after sample to be tested and antibody A and antibody B are sufficiently mixed, is incubated for obtain target complex；Institute
The excretion body antibody that antibody A is chemiluminescent molecule or the enzyme label that substrate can be made luminous is stated, the antibody B is marked by magnetic bead
Excretion body antibody；At least a kind of in the antibody A and antibody B is excretion body PD-L1 antibody；The target complex is antibody
A, the double-antibody sandwich compound that PD-L1 excretion body and antibody B are formed；
(2) magnetic separates: magnet being adsorbed onto the hollow pipe outer wall of porous foam metal filling, sets the porous foam metal
In magnetic field environment；The hollow tube that the Incubating Solution that step (1) obtains is filled by porous foam metal again, makes the double antibody
Sandwich complex is adsorbed on porous foam metal；
(3) double-antibody sandwich compound is eluted: with the inner cavity for the hollow tube that buffer solution for cleaning porous foam metal is filled；It removes again
The magnet of the hollow pipe outer wall of porous foam metal filling, and afford double-antibody sandwich compound；
(4) luminous intensity: the luminous intensity or double antibody folder for the double-antibody sandwich compound that detecting step (3) elution obtains is detected
The intensity that heart compound keeps substrate luminous calculates the concentration of excretion body PD-L1 antibody in sample to be tested.
6. application as claimed in claim 5, which is characterized in that the porous foam metal is porous foam iron, porous foam cobalt
Or porous foam nickel；
Preferably, the diameter of the porous foam metal Hole is 10um-100um.
7. application as claimed in claim 5, which is characterized in that the chemiluminescent molecule is acridine rouge, described substrate to be made to send out
The enzyme of light is horseradish peroxidase.
8. application as claimed in claim 5, which is characterized in that the magnetic bead is paramagnetic beads, diameter 10nm-1000nm.
9. application as claimed in claim 5, which is characterized in that liquid absorption device or liquid-transfering gun are utilized in the step (2), with upper and lower
Mode is drawn back and forth, and the hollow tube that the Incubating Solution that step (1) obtains is filled by porous foam metal presss from both sides the double antibody
Heart compound is adsorbed on porous foam metal.
10. application as claimed in claim 5, which is characterized in that liquid absorption device or liquid-transfering gun are utilized in the step (3), it is above
The resorption that gets off takes mode, the inner cavity of the hollow tube of cleaning porous foam metal filling.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111537726A (en) * | 2020-05-29 | 2020-08-14 | 武汉大学 | Method for efficiently and quantitatively detecting PD-L1 level in extracellular vesicle, ELISA kit and using method |
| CN111812326A (en) * | 2020-07-23 | 2020-10-23 | 四川携光生物技术有限公司 | Kit for quantitatively detecting content of PD-L1 and PD-1 conjugate |
| CN112379098A (en) * | 2020-11-05 | 2021-02-19 | 集美大学 | ELISA detection method for mimic enzyme-labeled antibody of histamine content in aquatic product |
| CN113866407A (en) * | 2021-12-01 | 2021-12-31 | 上海思路迪医学检验所有限公司 | Magnetic immunochemiluminescence detection kit for exosome HER2 protein |
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| CN106366195A (en) * | 2016-08-31 | 2017-02-01 | 上海美吉生物医药科技有限公司 | PD-L1 antibody immunomagnetic beads and preparation method thereof |
| CN106841613A (en) * | 2017-01-18 | 2017-06-13 | 上海良润生物医药科技有限公司 | A kind of method and system for detecting excretion body |
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| US6159378A (en) * | 1999-02-23 | 2000-12-12 | Battelle Memorial Institute | Apparatus and method for handling magnetic particles in a fluid |
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| CN111537726A (en) * | 2020-05-29 | 2020-08-14 | 武汉大学 | Method for efficiently and quantitatively detecting PD-L1 level in extracellular vesicle, ELISA kit and using method |
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| CN112379098A (en) * | 2020-11-05 | 2021-02-19 | 集美大学 | ELISA detection method for mimic enzyme-labeled antibody of histamine content in aquatic product |
| CN113866407A (en) * | 2021-12-01 | 2021-12-31 | 上海思路迪医学检验所有限公司 | Magnetic immunochemiluminescence detection kit for exosome HER2 protein |
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Application publication date: 20190521 |
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