CN206494924U - High flux pattern inducing cell screening chip - Google Patents
High flux pattern inducing cell screening chip Download PDFInfo
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- CN206494924U CN206494924U CN201720000002.XU CN201720000002U CN206494924U CN 206494924 U CN206494924 U CN 206494924U CN 201720000002 U CN201720000002 U CN 201720000002U CN 206494924 U CN206494924 U CN 206494924U
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- chip
- culturing room
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Abstract
The utility model provides a kind of high flux pattern inducing cell screening chip, it is characterised in that：The chip is rectangular, in a short side termination of rectangle, is machined with 4 injection orifices, is above machined with paired culturing room, and culturing room bottom, which is machined with, is evenly distributed with paired same shape, and the different cell hole of size.The utility model beneficial effect is that chip can flexibly set more passages and culturing room according to requirement of experiment, strengthens culture systems.It can detect multiple indexs in the limited area of chip, reach instant, effective, trace detection.Can in real time it be monitored within the period of detection, control nutriment or the input dosage for investigating medicine, it is ensured that the reliability of detection so that result can more react real cell growth environment, reach the testing goal finally needed.
Description
Technical field
The utility model is related to a kind of biochip of the cell culture of cell screening experiment, from using being a kind of
High flux pattern inducing cell screening chip.
Background technology
Biochip is nearly new and high technology developed rapidly in biomedical engineering field for 20 years.Because its
High flux, miniaturization and automate the characteristics of, meet quick, efficient, inexpensive industrialization implementing principle, medical diagnosis on disease,
The fields such as drug screening, cell culture, with very powerful utilization prospect.At present, in research multiple patterns to all kinds of dry thin
The experiment of the differentiation and proliferation of born of the same parents is relatively more, but the pattern for doing such experiment is more complicated, at the same versus experiment to phase
Requirement with experiment condition is also very harsh, it is desirable to have a kind of equipment of such experiment easy to process.Biochip applies to this
Class is tested, and there is provided scheme to solve this problem.
Therefore, contrast experiment's chip and the user of a kind of high flux screening of pattern inducing cell of our utility models
Method.
The content of the invention
To solve the above problems, the technical solution of the utility model is：A kind of high flux pattern inducing cell screening chip.
The chip is rectangular, in a short side termination of rectangle, is machined with 4 injection orifices, and 2. 1. injection orifice be one group with injection orifice,
4. 3. injection orifice be one group with injection orifice, along rectangular two long sides, symmetrical arrangement；The injection orifice that one group of each two connects
Road is connected to be flowed in two symmetrical interface channels in horizontal " Y " font to chip center direction.Two or so pairs
Parallel to the long side of chip after the interface channel turn of title, stretch to symmetrical with 2 after the segment distance of the other end one of chip
Curved channel is connected, two curved channel semicirculars in shape, and the top of semicircle is towards injection orifice one end of chip, semicircle bottom two
The termination of curved channel connects the circular culturing room of 2 first order.Processed respectively in the opposite side of 2 circular culturing room's correspondence imports
There is one outlet, outlet is connected with an inverted T shaped split channel, each inverted T shaped split channel connects 2 second level circles respectively again
Shape culturing room partners, the circular culturing room in totally two pairs of 4 second level.The circular culturing room in two pairs of second level and inverted T shaped shunting are logical
The corresponding other end of road import, is each machined with outlet, and one section of straight channel of outlet connection, 4 straight channels are connected to one and its
On vertical transverse passage-way.Another short side termination of described rectangular dies correspondence injection orifice one end, is machined with 1 outlet, goes out sample
Mouth 1 stock layout passage of connection, the other end of stock layout passage is vertically connected on the middle position of transverse passage-way length.
In above-mentioned technical proposal, the circular culturing room of 2 first order of the chip is symmetrical, and culturing room bottom is machined with
Equally distributed big round cell hole and small round cell hole；The bottom of the circular culturing room in two pairs of 4 second level, side
It is machined with big rectangle cell hole and small rectangle cell hole for a pair, a pair of other side are machined with big three horn cellses hole and small triangle
Cell is cheated.
In above-mentioned technical proposal, big the round cell hole and small round cell hole, big rectangle cell hole and small rectangle are thin
The surface in born of the same parents hole, big three horn cells hole and small three horn cells hole has all carried out hydrophilic treated, and is coated with promotion cell adherence
The factor of growth.The circular culturing room of the first order of the chip, the circular culturing room in the second level and all kinds of passages are dredged
Water process.The big round cell hole and small round cell hole, big rectangle cell hole and small rectangle cell hole, big three horn cells hole
It is made, or is processed by double-layer chip laminating by secondary operation with small three horn cells hole, the geomery energy in cell hole
Enough accurate processing.
In above-mentioned technical proposal, the injection orifice interface channel of the chip is 1/4 circular arc, and is symmetrically and evenly distributed two-by-two
Both sides in chip one end.Quantity, series and the circle of circular culturing room of the circular culturing room of the first order of the chip and the second level
The shape in shape culturing room bottom cell hole, is not limited to above-mentioned 6 circular culturing room, two-stage, 6 kinds of cell hole shapes.It is described thin
The bottom in born of the same parents hole is less than circular culturing room bottom, and circular culturing room bottom is less than each channel floor 0.25mm-3mm.
The purpose of this utility model is, using on the good cell culture basis of biochip, by the improvement of structure, to increase
Plus its application function.Can there is provided difference by being operated on a kind of chip（It is identical）Cell growth environment, other change
Under the conditions of amount is all immovable, control certain variable that can be single can play simultaneous quantitative orientation observation contrast, so that training
The foster cycle shortens, and reduces the experimental period and error observed immediately.
The utility model technical characterstic be by controlling micropump, from the cell required for 4 injection port injection experimentses and
Nutrient solution.Corresponding nutriment and medicine periodically can be fed by 4 injection ports, between the time that control nutrient solution is shipped to
Every, it is ensured that cell is normally metabolic.The passage of UNICOM is by cell and nutriment or medicament transport to culturing room, cell
Grown herein, immediately observation.Under specific condition of culture, each culturing room can play a part of a contrast.
Compared with existing same experiments, the utility model chip has following beneficial effect：It can set more logical
Road and circular culturing room, strengthen culture systems.Multiple indexs can be detected in the limited area of chip, reach it is instant, effective,
Trace detection.Can in real time it be monitored within the period of detection, the time interval of control nutriment or investigation medicine is defeated
Enter dosage and speed, it is ensured that the reliability of detection so that result can more react real cell growth environment, reach final needs
Testing goal.
Brief description of the drawings
Fig. 1 is schematic diagram of the present utility model.
Fig. 2 is culturing room of the present utility model and cell hole, the tangent plane schematic diagram of passage.
In figure：1. chip；2. big rectangle cell hole；3. small rectangle cell hole；4. big round cell hole；5. curved channel；
6. injection orifice is 1.；7. injection orifice interface channel；8. injection orifice is 2.；9. interface channel（8）；10. injection orifice is 3.；11. injection orifice
④；12. first order circle culturing room；13. small round cell is cheated；14. inverted T shaped split channel.15. second level circle culturing room；
16. big three horn cellses hole；17. small three horn cellses hole；18. straight channel；19. stock layout passage；20. outlet；21. transverse passage-way；22.
Culturing room；23. cell is cheated；24. passage.
Embodiment
Further the utility model is illustrated with reference to the accompanying drawings and examples.
Specific embodiment one
Referring to figs. 1 to Fig. 2 chip form structures, chip has 2 grades of culturing room 22.First order circle culturing room 12 and the second level
Circular culturing room 15, and all kinds of passages 24, have carried out hydrophobic treatment.First order circle culturing room is symmetrical, culturing room
Bottom is machined with equally distributed big round cell hole 4 and small round cell hole 13.A pair of the circular culturing room in two pairs of 4 second level
Big three horn cellses hole 16 and small three horn cells hole 17 are processed, another pair processes big rectangle cell hole 2 and small rectangle cell hole 3.Carefully
Born of the same parents cheat 23 basal surface hydrophilic treateds, and are coated with FN albumen.
All passages：It is wide=150 μm, it is high=100 μm；All circular 22 diameters of culturing room=10mm；The first order：Great circle
The cell in shape cell hole 4 cheats 23 radius r=65 μm, deep=50 μm；The cell in small round cell hole 13 cheats 23 radius r=25 μm, deeply=
50µm.The second level：Each side size of big rectangle, long=350 μm, wide=60 μm, deep 50 μm, small each side size of rectangle is long=150 μm, wide
It is=20 μm, deep 50 μm.The equilateral triangle size in big three horn cellses hole 16 is the length of side=30 μm, the equilateral triangle in small three horn cellses hole 17
Size is the length of side=10 μm, and the bottom of culturing room 22 is less than each passage 24 bottom 3mm.
In experimentation, 4 injection ports correspond to 4 interfaces of Micropump, the phase required for injection port injection experimentses respectively
With stem cell and Different Nutrition liquid.Corresponding nutriment periodically can be fed by 4 injection ports, control nutrient solution is shipped to
Speed.Then transported by passage, it is stand-by that nutriment is directly taken culture indoor storage.Make cell raw in culturing room
It is long.After cell growth for a period of time after, can directly pass through the chip and complete ensuing contrasting detection and work.Detect a certain medicine
Influence to same cell growth.
Specific embodiment two
Referring to figs. 1 to Fig. 2 chip form structures, chip has 3 grades of culturing room 22.A pair of the 2 square length of side=5mm of the first order
Culturing room 22, the 4 circle=5mm in second level culturing room 22, four pairs of the third level totally 8 rhombus length of side=5mm culturing room
22, and all kinds of passages 24, carry out hydrophobic treatment.The square culturing room bottom of the first order is machined with equally distributed great circle
Shape cell hole 4 and small round cell hole 13.Circular a pair of big three horn cellses of processing of culturing room 22 in two pairs of 4 second level cheat 16 Hes
Small three horn cellses hole 17, another pair processes big rectangle cell hole 2 and small rectangle cell hole 3.Four pairs of 8 third level, 8 rhombus trainings
Room 22 is supported, is the cell hole 23 of big/small rhombus respectively, big/small square cell hole 23, the cell hole 23 of big/small big rectangle,
The cell hole 23 of greatly/small pentalpha, cell cheats 23 basal surface hydrophilic treateds, and is coated with FN albumen.
All passages：It is wide=250 μm, it is high=500 μm；The first order：The cell in big round cell hole 4 cheats 23 radius r=45 μm,
It is deep=45 μm；The cell in small round cell hole 13 cheats 23 radius r=15 μm, deep=45 μm.The second level：Each side size of big rectangle, long=
It is 150 μm, wide=20 μm, it is deep 45 μm；Small each side size of rectangle, it is long=80 μm, wide=10 μm, it is deep 45 μm.Big three horn cellses hole 16 is just
Triangle size is the length of side=30 μm, and the equilateral triangle size in small three horn cellses hole 17 is the length of side=10 μm.The third level：Size rhombus
Cell cheat 23 each side size=30 μm, deep 45 μm, the cell of small rhombus cheats 23 each side size=10 μm, deep 45 μm；Big square
Cell cheat 23 each side size=30 μm, small square cell cheats 23 each side size=10 μm；The cell of big rectangle cheats 23 each sides
Size, long=80 μm, wide=20 μm, deep 45 μm, the cell of small rectangle cheats 23 each side sizes, long=50 μm, wide=10 μm, deep 45 μm；
The cell of big pentalpha cheats 23 each side size=20 μm, deep 45 μm, and the cell of small pentalpha cheats 23 each side size=10 μm, deep
45µm.The bottom of culturing room 22 is less than each passage 24 bottom 2mm.
In experimentation, 4 injection ports correspond to 4 interfaces of Micropump respectively, required for injection port injection experimentses not
With stem cell and identical nutrient solution.Corresponding nutriment periodically can be fed by 4 injection ports, control nutrient solution is shipped to
Speed.Then transported by passage, it is stand-by that nutriment is directly taken culture indoor storage.Make cell raw in culturing room
It is long.After cell growth for a period of time after, can directly pass through the chip and complete ensuing contrasting detection and work.Detect a certain medicine
Influence to different cell growths.
Claims (4)
1. a kind of high flux pattern inducing cell screening chip, it is characterised in that：The chip（1）It is rectangular, the one of rectangle
Individual short side termination, is machined with 4 injection orifices, injection orifice is 1.（6）With injection orifice 2.（8）For one group, injection orifice is 3.（10）With injection
Hole is 4.（11）For one group, along rectangular two long sides, symmetrical arrangement；The injection orifice interface channel of one group of each two（7）
To chip center direction two symmetrical interface channels are flowed in horizontal " Y " font（9）In；Two symmetrical
Interface channel（9）Parallel to the long side of chip after turn, stretch to after the segment distance of the other end one of chip with 2 symmetrical arcs
Shape passage（5）It is connected；Two curved channels（5）Semicircular in shape, the top of semicircle is towards injection orifice one end of chip, semicircle bottom
Two curved channels（5）Termination connect the circular culturing room of 2 first order（12）；In 2 circular culturing room（12）Correspondence import
Opposite side be machined with one outlet respectively, outlet and an inverted T shaped split channel（14）Connection, each inverted T shaped split channel
（14）Connect the circular culturing room in 2 second level respectively again（15）Partner, the circular culturing room in totally two pairs of 4 second level（15）；
The circular culturing room in two pairs of second level（15）With inverted T shaped split channel（14）The corresponding other end of import, is each machined with outlet, goes out
Mouth one section of straight channel of connection（18）, 4 straight channels（18）It is connected to a perpendicular transverse passage-way（21）On；The rectangular core
Piece（1）Another short side termination of correspondence injection orifice one end, is machined with 1 outlet（20）；Outlet（20）1 stock layout of connection leads to
Road（19）, stock layout passage（19）Other end be vertically connected on transverse passage-way（21）The middle position of length.
2. a kind of high flux pattern inducing cell screening chip according to claim 1, it is characterised in that：The chip
（1）The circular culturing room of 2 first order（12）Symmetrical, culturing room bottom is machined with equally distributed big round cell hole
（4）With small round cell hole（13）；The circular culturing room in two pairs of 4 second level（15）Bottom, a pair of side are machined with
Big rectangle cell hole（2）With small rectangle cell hole（3）, other side is machined with big three horn cellses hole for a pair（16）With small triangle
Cell is cheated（17）.
3. a kind of high flux pattern inducing cell screening chip according to claim 1, it is characterised in that：The chip
（1）Big round cell hole（4）With small round cell hole（13）, big rectangle cell hole（2）With small rectangle cell hole（3）, big triangle
Cell is cheated（16）With small three horn cells hole（17）Surface all carried out hydrophilic treated, and be coated with promotion cell adherence growth
The factor；The chip（1）The circular culturing room of the first order（12）, the circular culturing room in the second level（15）And all kinds of passages（24）
Hydrophobic treatment is carried out；The big round cell hole（4）With small round cell hole（13）, big rectangle cell hole（2）With small square
Shape cell is cheated（3）, big three horn cells hole（16）With small three horn cells hole（17）It is made by secondary operation, or passes through double-layer chip
Laminating is processed, cell hole（23）Geomery can accurately process.
4. a kind of high flux pattern inducing cell screening chip according to claim 1, it is characterised in that：The chip
（1）Injection orifice interface channel（7）For 1/4 circular arc, and the both sides of chip one end are symmetrically and evenly distributed in two-by-two；The cell
Hole（23）Bottom be less than culturing room（22）Bottom, culturing room（22）Bottom is less than each passage（24）Bottom 0.25mm-3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720000002.XU CN206494924U (en) | 2017-01-01 | 2017-01-01 | High flux pattern inducing cell screening chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720000002.XU CN206494924U (en) | 2017-01-01 | 2017-01-01 | High flux pattern inducing cell screening chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206494924U true CN206494924U (en) | 2017-09-15 |
Family
ID=59802822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720000002.XU Withdrawn - After Issue CN206494924U (en) | 2017-01-01 | 2017-01-01 | High flux pattern inducing cell screening chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206494924U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701533A (en) * | 2017-01-01 | 2017-05-24 | 重庆科技学院 | Using method of high-flux pattern induced cell screening chip |
| CN106834083A (en) * | 2017-01-01 | 2017-06-13 | 重庆科技学院 | A kind of high flux pattern inducing cell screening chip |
-
2017
- 2017-01-01 CN CN201720000002.XU patent/CN206494924U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701533A (en) * | 2017-01-01 | 2017-05-24 | 重庆科技学院 | Using method of high-flux pattern induced cell screening chip |
| CN106834083A (en) * | 2017-01-01 | 2017-06-13 | 重庆科技学院 | A kind of high flux pattern inducing cell screening chip |
| CN106834083B (en) * | 2017-01-01 | 2018-10-23 | 重庆科技学院 | A kind of high throughput pattern inducing cell screening chip |
| CN106701533B (en) * | 2017-01-01 | 2018-10-23 | 重庆科技学院 | A kind of application method of high throughput pattern inducing cell screening chip |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170915 Effective date of abandoning: 20181023 |
|
| AV01 | Patent right actively abandoned |