CN206266634U - For the cell culture biochip of shearing force experiment - Google Patents
For the cell culture biochip of shearing force experiment Download PDFInfo
- Publication number
- CN206266634U CN206266634U CN201621278925.3U CN201621278925U CN206266634U CN 206266634 U CN206266634 U CN 206266634U CN 201621278925 U CN201621278925 U CN 201621278925U CN 206266634 U CN206266634 U CN 206266634U
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- chip
- cultivation region
- rectangle
- taper
- shearing force
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Abstract
Utility model provides a kind of cell culture biochip for shearing force experiment, it is characterised in that：Have 2 rows totally 4 injection orifices in rectangular chip one end, injection orifice and V passages along oblong chips two sides long, it is Y-shaped to be flowed to symmetrical 2 interface channels.2 interface channel is connected to 1 rectangle cultivation region and 1 taper cultivation region.Compared with existing design, the utility model chip has following beneficial effect, than common chip there is provided the culture systems of different shearing forces, apply different shearing forces in stem cell incubation, reaches instant, effective, micro observation.Real-time monitoring can be carried out within the time period of detection, the input rate of nutriment is controlled, it is ensured that the reliability of detection so that result can more react real stem cell growth environment, grasp the influence that shearing force is broken up to stem cell.
Description
Technical field
The utility model is related to a kind of biochip of novel cell culture, from using being that one kind is used in cell
The cell culture biochip for shearing force experiment in culture experiment.
Background technology
Biochip is nearly new and high technology for being developed rapidly in biomedical engineering field for 20 years.Biological core
Piece is widely used, life science and put into practice, medical research and clinic, drug design, environmental protection, agricultural, military affairs etc.
Every field.Because its high flux, the characteristics of being miniaturized and automate, meet quick, efficient, inexpensive industrialization running former
Then, in fields such as medical diagnosis on disease, drug screening, cell culture, with very powerful utilization prospect.These will undoubtedly be produced
Huge social and economic benefit.However, traditional biochip technology has many insoluble, main performance
In vitro gene and protein molecular is used in conventional bio-chip, is experienced from intracellular environment extracting and developing, purification, table
After a series for the treatment of of face adhesion, molecule combination etc., the cell after treatment changes, and can not play observation in time, point
The effect of analysis.So, to change this situation, it is necessary to fundamentally thoroughly change the mentality of designing of biochip, design is a kind of
The chip of Real Time Observation contrast can be formed.
In the differentiation and proliferation research to cell, cyto-mechanics experiment is often used, and fluid in analogue body, cell is probed into
Influence to different shearing forces is one therein, but lacks the cell culture biochip for shearing force experiment at present,
Reach the real-time reflection situation that can obtain the cell under the conditions of simulation hydrodynamics to shearing force.
Accordingly, it would be desirable to utility model provides a kind of cell culture biochip for shearing force experiment, it is above-mentioned to solve
Problem.
The content of the invention
Technology contents of the present utility model are a kind of cell culture biochips for shearing force experiment, and its feature exists
In：There are 2 rows totally 4 injection orifices in rectangular chip one end, injection orifice is two-by-two a pair, and 2 injection orifices of each pair are each distinguished
One totally 2 V passage of connection, 2 V passages along oblong chips two sides long, it is Y-shaped to be flowed to symmetrical 2
Interface channel, Y-shaped opening is towards the both sides of chip.Parallel to the side long of chip after 2 interface channels turns, chip is stretched to
The other end；2 interface channels in left and right are connected to 1 rectangle cultivation region and 1 taper cultivation region, two at the middle part of chip
Individual cultivation region is symmetrically distributed in the both sides in the middle part of chip.The two cultivation region Main Functions are culture living cell growths, for examining
Survey living cells.2 cultivation region other ends, are respectively connected with interface channel, and 2 interface channels are finally flowed in the chip other end
1 rectangle waste liquid pool in portion, rectangle waste liquid pool is connected by passing away with outlet.
Preferably, the rectangle cultivation region of the chip and the shape of taper cultivation region all maintain the right angle at turning, with
Influence of the simulation vortex to cell.The cone of the taper cultivation region of chip has towards rectangle waste liquid pool one end and cone towards injection orifice
2 kinds of specifications of one end.Experimental comparison and the checking of data are easy in so design.
Preferably, the rectangle cultivation region is different with taper cultivation region inner width, can under identical culture flow velocity,
Because rectangle cultivation region is different with the width of taper cultivation region, shearing force inside rectangle cultivation region and taper cultivation region is caused not
Together.Cell is cultivated in rectangle cultivation region and taper cultivation region, and comparative analysis goes out in the presence of different shearing forces does thin
The differentiation situation of born of the same parents, and can be calculated according to hydrodynamics formula according to requirement of experiment, cutting under the different width of research on adjustment
Stress, research is identical or differs influence of the shear stress change produced under flow velocity to cell.But rectangle cultivation region and cone
The floor height of shape cultivation region, at import and exit height is all identical, i.e., when there is the gradient bottom surface, rectangle cultivation region and taper
The gradient of two bottom surfaces of cultivation region is all consistent.So it is easy to ensure the contrast of experimental data.
Preferably, the making material of the chip is all transparent material, so ensures that chip can be under microscope or CCD
Detection.The chip is all machined with grid groove on the region of rectangle cultivation region and taper cultivation region, in order to cellular localization
To the positioning of cell shearing power during with fluid simulation.
The advantage of the utility model chip：Under good cell culture environment, by the experimental implementation on chip, energy
The experimental observation contrast of simultaneous quantitative orientation is enough played, so that influence experiment of the research shearing force to cell more facilitates, is subtracted
The experimental period and error of instant observation are lacked.Compared with existing design, the utility model chip has following beneficial effect：
Than common chip there is provided the culture systems of different shearing forces, apply different shearing forces in stem cell incubation, reach
Immediately, effective, micro observation.Real-time monitoring can be carried out within the time period of detection, the input rate of nutriment is controlled, protected
Demonstrate,prove the reliability of detection so that result can more react real stem cell growth environment, grasp what shearing force was broken up to stem cell
Influence, is that follow-up work is prepared.
Brief description of the drawings
Fig. 1 is a kind of schematic top plan view of specification of the present utility model.
Fig. 2 is a kind of schematic top plan view of specification of the present utility model.
Wherein：1. rectangle waste liquid pool；2. passing away；3. rectangle cultivation region；4. interface channel；5.V passages；6. inject
Hole；7. taper cultivation region；8. outlet；9. grid groove.
Specific embodiment
1-2 is further illustrated to the utility model below in conjunction with the accompanying drawings.
Operating procedure is as follows：Stem cell and nutrient solution are injected in pairs respectively from the injection orifice 6 of rectangular chip one end, it is fixed
Phase feeds corresponding nutriment by injection orifice 6, it is ensured that cell is normally metabolic.Injection orifice 6 is grouped in pairs, as
Control group.Nutriment is directly transported in cultivation region, for the growth of observation cell immediately.After cell attachment grows,
Cell culture fluid is persistently injected from two pairs of injection orifices 6, the nutrient solution of fluidised form flows through rectangle cultivation region 3 and taper cultivation region 7 and produces
Shearing force in cell.Under specific experiment condition of culture, rectangle cultivation region 3 and taper cultivation region 7 can play right
It is compared to use.Cell divides under it after cell attachment growth 24h, can directly pass through high-definition camera or DVD detection shearing forces
Change situation.Grid groove 9 plays the cellular localization effect at chip different fluid position.Rectangle waste liquid pool 1 will do thin in culture pond
The space of the metabolite of born of the same parents or unnecessary injection mass temporarily storage, it is ensured that the normal culture environment of stem cell.Finally
The waste liquid of rectangle waste liquid pool 1 is discharged by passing away 2 with final outlet 8.
Claims (4)
1. it is a kind of for shearing force experiment cell culture biochip, it is characterised in that：There are 2 rows in rectangular chip one end
Totally 4 injection orifices（6）, injection orifice（6）It is two-by-two a pair, 2 injection orifices of each pair（6）Totally 2 V passage is each connected respectively
（5）, 2 V passages（5）It is Y-shaped to be flowed to symmetrical 2 interface channels along two sides long of oblong chips（4）；2
Bar interface channel（4）Parallel to the side long of chip after turn, the other end of chip is stretched to；2 interface channels in left and right（4）In chip
Middle part, be connected to 1 rectangle cultivation region（3）With 1 taper cultivation region（7）, two cultivation regions are symmetrically distributed in chip
The both sides at middle part；2 cultivation region other ends, are respectively connected with interface channel（4）, 2 interface channels（4）Finally it is flowed in chip
1 rectangle waste liquid pool in the middle part of the other end（1）, rectangle waste liquid pool（1）By passing away（2）With outlet（8）Connection.
2. it is according to claim 1 it is a kind of for shearing force experiment cell culture biochip, it is characterised in that：Chip
Rectangle cultivation region（3）With taper cultivation region（7）Shape all maintain the right angle at turning；The taper cultivation region of chip（7）'s
Cone has towards rectangle waste liquid pool（1）One end and cone towards injection orifice（6）2 kinds of specifications of one end.
3. it is according to claim 1 it is a kind of for shearing force experiment cell culture biochip, it is characterised in that：Rectangle
Cultivation region（3）With taper cultivation region（7）Inner width is different；But rectangle cultivation region（3）With taper cultivation region（7）Bottom surface it is high
Degree, at import and exit height it is all identical, i.e., when there is the gradient bottom surface, rectangle cultivation region（3）With taper cultivation region（7）Two
The gradient of individual bottom surface is all consistent.
4. it is according to claim 1 it is a kind of for shearing force experiment cell culture biochip, it is characterised in that：It is described
The making material of chip is all transparent material；The chip is all machined with net on the region of rectangle cultivation region and taper cultivation region
Lattice groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621278925.3U CN206266634U (en) | 2016-11-27 | 2016-11-27 | For the cell culture biochip of shearing force experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621278925.3U CN206266634U (en) | 2016-11-27 | 2016-11-27 | For the cell culture biochip of shearing force experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206266634U true CN206266634U (en) | 2017-06-20 |
Family
ID=59041530
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621278925.3U Expired - Fee Related CN206266634U (en) | 2016-11-27 | 2016-11-27 | For the cell culture biochip of shearing force experiment |
Country Status (1)
| Country | Link |
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| CN (1) | CN206266634U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434347A (en) * | 2016-11-27 | 2017-02-22 | 重庆科技学院 | Cell culture biological chip for shear force experiment |
| CN106544272A (en) * | 2016-11-27 | 2017-03-29 | 重庆科技学院 | A kind of using method of the biochip for shearing force experiment |
-
2016
- 2016-11-27 CN CN201621278925.3U patent/CN206266634U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434347A (en) * | 2016-11-27 | 2017-02-22 | 重庆科技学院 | Cell culture biological chip for shear force experiment |
| CN106544272A (en) * | 2016-11-27 | 2017-03-29 | 重庆科技学院 | A kind of using method of the biochip for shearing force experiment |
| CN106434347B (en) * | 2016-11-27 | 2018-10-23 | 重庆科技学院 | A kind of cell culture biochip for shearing force experiment |
| CN106544272B (en) * | 2016-11-27 | 2019-07-23 | 重庆科技学院 | A kind of application method of the biochip for shearing force experiment |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170620 Termination date: 20211127 |
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| CF01 | Termination of patent right due to non-payment of annual fee |