CN206266579U - Drug screening biochip with air chamber - Google Patents
Drug screening biochip with air chamber Download PDFInfo
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- CN206266579U CN206266579U CN201621278792.XU CN201621278792U CN206266579U CN 206266579 U CN206266579 U CN 206266579U CN 201621278792 U CN201621278792 U CN 201621278792U CN 206266579 U CN206266579 U CN 206266579U
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- air chamber
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- drug screening
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Abstract
Utility model provides a kind of drug screening biochip with air chamber.It is made up of two groups of injection ports, two cultivation regions, a shared air chamber, a waste liquid pool and bending interface channel.It is characterized in：Chip upper piece be machined with since gas access, connection air chamber passage, air chamber, the structure terminated to gas vent, these structures are in a connection straight line arrangement；The both sides of correspondence upper piece this connection straight line, two groups of " V " type sample intake passages, two cultivation regions are symmetrily processed with bottom sheet, on correspondence upper piece this connection straight line, waste liquid pool, passing away, outlet are machined with bottom sheet.The utility model utilizes biochip, basis with good cell culture, by the improvement of structure, microflow control technique is combined with cell culture technology and is applied to drug screening field, identical cell growth environment is provided on a single die, is realized under the conditions of its dependent variable is all immovable, it is single to change administration species or dosage, by observing the change of the physical signs such as the cellular morphology in the case where medicine is acted on, the purpose of medicine is screened.
Description
Technical field
The utility model is related to a kind of biological microanalysis chip of newtype drug screening, from using being a kind of utilization
In living cells culture systems, a kind of new drug screening biochip with air chamber.
Background technology
High-flux medicaments sifting refer to based on the experimental technique of molecular level and cellular level, using microplate format as
Experimental tool carrier, process of the test is performed with automation operating system, with sensitive quick detecting instrument collection experimental result number
According to, treatment is analyzed to experimental data with computer, same time logarithm with ten million sample detection, and with corresponding database
Support the technical system of overall system's operating.High-flux medicaments sifting system is the one of new drug development research in original new drug screening
Individual key areas, have been widely used in the screening of candidate compound microbic activity.The Dominant Plat not only may be used
For finding new drug, it can also be used to drug research.But conventional medicament screening chip is unable under the effect of real-time monitored medicine
Cellular morphology changes.This problem is solved, need to be by multiple steps such as the addition of medicine, the culture of experimental cell, detection of effect of drugs
Suddenly chip piece is integrated into, realizes the automated analysis of drug screening, reduce the experimental period and error of observation immediately.
Therefore, a kind of biological microanalysis chip of the drug screening with air chamber of the utility model, to solve the above problems.
The content of the invention
The purpose of this utility model is to design a kind of biological microanalysis core of the drug screening of living cells culture using air chamber
Piece, medicine is integrated on chip piece to multiple experiential functions such as sample, cell culture, effect of drugs detections.
The technical scheme of this experiment is：A kind of drug screening biochip with air chamber is provided.By two groups of injection ports, two
Individual cultivation region, a shared air chamber, a waste liquid pool and bending interface channel composition.It is characterized in：Chip upper piece processing
Have since gas access, connection air chamber passage, air chamber, the structure terminated to gas vent, these structures are in a connection
Straight line is arranged；Correspondence upper piece this connection straight line both sides, bottom sheet be symmetrily processed with two groups of " V " type sample intake passages, two training
Area is supported, this is connected on straight line correspondence upper piece, and waste liquid pool, passing away, outlet are machined with bottom sheet.Arranged along a connection straight line
Cloth, and along this straight line symmetry arrangement, can intensive utilization chip well space, and utility model can be used
A kind of drug screening biochip with air chamber as functional unit, connection composition is of different shapes, expand number of experiments or
The biochip of function.Two groups of injection ports are machined with the bottom sheet of chip body, every group of injection port has includes cell and its training
" V " the type injection port one and " V " type injection port two of nutrient solution import or Imported Medicines, injection port are connected with straight channel is connected.When
After infusion of medicine, medicine is sufficiently mixed at " V " type passage and acts on cell with cell sample.Two cultivation regions simultaneously with gas
Room is connected, and air chamber conveys gas needed for cell culture and ensures that quantity delivered is identical by the airway that air chamber and cultivation region connect.
Respectively there is " U " type structure inside two cultivation regions, when nutrient solution and medicine are reached, " U " type structure can intercept cell,
Ensure cell in fixed region growing, and the liquid such as nutrient solution and medicine can be with free flow.
In above-mentioned technical proposal, the cultivation region, two cultivation region sizes are consistent, are deep 1mm-2mm, diameter 2mm-
The drum-shaped of 5mm.So design ensures the in the same size of cell culture area.It is rhombus, height 0.5mm, the length of side that air chamber is overlooked
≥2mm.So design is easy to the connection of each connecting tube just on four angles of rhombus, it is to avoid air chamber because there is corner, generation
Air whirl, is unfavorable for the exclusion of waste gas.Waste liquid pool is cuboid, depth be 1mm-2mm, short side it is long >=5mm.Design ensure that
Waste liquid pool can at least accommodate two waste liquids for once producing of cultivation region.In above-mentioned technical proposal, the chip upper piece, under
Piece and chip base are transparent material.It is in cuboid, passing away and gas vent phase with waste liquid that waste liquid pool is overlooked
Even, it is by the space of two metabolites or unnecessary injection mass of cultivation region inner cell temporarily storage, it is ensured that thin
The normal culture environment of born of the same parents.
In above-mentioned technical proposal, the cultivation region, in the side of curved channel two that two cultivation regions are connected with waste liquid pool, with
Cylindrical cultivation region inwall is respectively machined with " U " type structure at 5 μm, and " U " type structure is a width of 30 μm -60 μm, etc.
Higher than the semicircular arc wall of cultivation region；The curved channel one and curved channel two are 1/4 circular arc, and in pairs, it is right
Title is distributed in a both sides for connection straight line.The function of " U " the type structure inside cultivation region, makes cell be given birth in fixed region
It is long, it is easy to real-time monitored in different pharmaceutical（Or the medicine of various concentrations）The situation of change of the lower cellular morphology of effect.
In above-mentioned technical proposal, the turbine structure is that two semicircle dislocation form serpentine passage relatively；Turbine structure position
Conflux in 2 " V " type passages and intersect at 1 vertex (vertices) of " V " word of passage, two semicircle spacing distances are " V " type channel width
The 1/2 of degree, beneficial to the mixing of liquid." V " type passage is embedded with the turbine structure of liquid in hybrid channel, after infusion of medicine with
Cell sample is sufficiently mixed at " V " type passage, it is ensured that medicine is fully contacted and has an effect with cell；Or two are added simultaneously
Medicine is planted, is sufficiently mixed at " V " type passage, it is ensured that medicine is well mixed.
In above-mentioned technical proposal, the passage of the bottom sheet processing, whole a height of 800 μm -1000 μm, a width of 300 μm of -600 μ
m.Channel size is that cell size and quantity determine that purpose keeps the homogeneity of experiment, is also beneficial to not according to needed for experiment
With the requirement of cell experiment.
In above-mentioned technical proposal, the biochip is divided into 3 layers, most last layer be upper piece, be that sampling device and gas are logical
Road, including, gas access, air chamber passage, airway, air chamber, gas vent, and insertion upper piece and middle " V " type injection port
First, " V " type injection port two, outlet；Middle one layer is bottom sheet, predominantly liquid communication pipeline, including " V " type passage, turbine knot
Structure, straight channel, curved channel one, cultivation region, curved channel two, waste liquid pool, " U " type structure；Bottom is chip base.This reality
With new utilization biochip, the basis with good cell culture, by the improvement of structure, by microflow control technique and cell
Culture technique is combined and is applied to drug screening field.Identical cell growth environment is provided on a kind of chip,
It is single to change administration species or dosage under the conditions of its dependent variable is all immovable, it is thin in the case where medicine is acted on by observing
Born of the same parents' metamorphosis, reaches the purpose of drug screening.
Effect feature of the present utility model：The utility model chip is by the addition of medicine, experimental cell culture, effect of drugs
Multiple steps such as detection are integrated on chip piece body, and multiple indexs can detects in limited area, reach it is instant, effective,
Trace detection.Real-time monitoring can be carried out within the time period of detection, the input rate of nutriment is controlled, it is ensured that detection can
By property so that result can more react real cell growth environment, final drug screening purpose is reached.
Brief description of the drawings
Fig. 1 is overlooking the structure diagram of the present utility model.
Fig. 2 is overlooking the structure diagram A-A sectional views of the present utility model.
Fig. 3 is the hybrid turbine schematic enlarged-scale view in local " V " type interface channel.
Wherein：1. " V " type injection port one；2. " V " type passage；3. gas access；4. air chamber passage；5. turbine structure；6.
Curved channel one；7. cultivation region；8. airway；9. air chamber；10. waste liquid pool；11. passing aways；12. outlets；13. chip bases
Bottom；14. gas vents；15. curved channels two；16. " U " type structures；17. " V " type injection ports two；18. straight channels；
19. upper piece；20. bottom sheet.
Specific embodiment
1-3 and embodiment are further illustrated to the utility model below in conjunction with the accompanying drawings.
Specific embodiment one
Referring to figs. 1 to the chip form structure of Fig. 3, a height of 800 μm, a width of 300 μm of the passage of the fluid of chip bottom sheet 20.
There is " U " type structure 16 for a width of 60 μm of semicircular arc walls each cultivation region 7 diameter 5mm, centre, itself and cylindrical cultivation region
7 inwalls are at 5 μm.
Operating procedure is as follows：High purity inert gas first are passed through in gas access 3 with syringe pump, gas is arrived through air chamber passage 4
Up to air chamber 9, then foreign gas is discharged through gas vent 14.Syringe pump is used again in two " V " type injection ports 1 and " V " of chip
Type injection port 2 17 is passed through three-level deionized water, and deionized water enters cultivation region 7 by turbine structure 5, curved channel 1, around
Cross " U " type structure 16, waste liquid pool 10 is entered by curved channel 2 15, continue to flow into passing away 11, pumping is used in outlet 12
Go out.It is continuous to be passed through three-level deionized water 2h to exclude material remaining in chip.Then, according to the medicine and medicine of drug screening
The pathology tumour cell of object, by syringe pump, will screen medicine or target cell from " V " type injection port 1 or " V " type sample introduction
Mouthfuls 2 17 each cell culture area 7 for importing chips, carry out culture, medicine irritation and the cell phychology of equivalent target cell with
The observation of germiparity.Finally injection deionized water cleaning.
Specific embodiment two
The chip form structure of reference picture 1- Fig. 3, a height of 1000 μm, a width of 600 μm of the passage of the fluid of chip bottom sheet 20.
There is " U " type structure 16 for a width of 30 μm of semicircular arc walls each cultivation region 7 diameter 2mm, centre, itself and cylindrical cultivation region
7 inwalls are at 5 μm.
Operation is with specific embodiment one.
Claims (6)
1. a kind of drug screening biochip with air chamber, by upper piece（19）, bottom sheet（20）, chip base（13）Three part groups
Into, it is characterised in that：Chip upper piece（19）It is machined with from gas access（3）Start, connect air chamber passage（4）, air chamber
（9）, to gas vent（14）The structure of end, these structures are in a connection straight line arrangement；Correspondence upper piece（19）This connection
The both sides of straight line, in bottom sheet（20）It is symmetrily processed with two groups of " V " type sample intake passages, two cultivation regions（7）, correspondence upper piece（19）This
On bar connection straight line, in bottom sheet（20）It is machined with waste liquid pool（10）, passing away（11）, outlet（12）；Every group of " V " type sample introduction leads to
Road is by " V " type injection port one（1）, " V " type injection port two（17）2 injection ports, and 2 " V " type passages（2）Connection conflux and
Into；Two groups of opening above pieces of " V " type sample intake passage（19）Connection straight line be symmetry axis, outward opening symmetrically arranges in " water " word
Cloth；In every group of 2 " V " type passages（2）River outlet, is machined with turbine structure（5）；Every group of symmetrical " V " type passage（2）Conflux
Afterwards, respectively with 2 parallel to upper piece（19）Connect the straight channel of straight line（18）It is connected, straight channel（18）With curved channel one（6）
Connection；Curved channel one（6）The other end and cultivation region（7）One end connects, cultivation region（7）The other end passes through curved channel two
（15）It is connected to waste liquid pool（10）；Waste liquid pool（10）By passing away（11）With outlet（12）Connection；Air chamber（9）By two
Airway（8）Respectively with two cultivation regions（7）Be connected, i.e., two cultivation regions（7）By two airways（8）Share air chamber（9）.
2. a kind of drug screening biochip with air chamber according to claim 1, it is characterised in that：The cultivation region
（7）, two cultivation regions（7）Size is consistent, is deep 1mm-2mm, the drum-shaped of diameter 2mm-5mm, air chamber（9）It is water chestnut to overlook
Shape, height 0.5mm, the length of side >=2mm, waste liquid pool（10）Be cuboid, depth be 1mm-2mm, short side it is long >=5mm.
3. a kind of drug screening biochip with air chamber according to claim 1, it is characterised in that：The cultivation region
（7）, two cultivation regions（7）With waste liquid pool（10）Connected curved channel two（15）Side, with cylindrical cultivation region（7）Inwall
At 5 μm, " U " type structure is respectively machined with（16）, " U " type structure（16）It is a width of 30 μm -60 μm, waits higher than cultivation region
（7）Semicircular arc wall；The curved channel one（6）With curved channel two（15）1/4 circular arc is, and in pairs, it is right
Title is distributed in piece（19）Connect the both sides of straight line.
4. a kind of drug screening biochip with air chamber according to claim 1, it is characterised in that：The turbine knot
Structure（5）For two semicircle dislocation form serpentine passage relatively；Turbine structure is located at 2 " V " type passages（2）Confluxing, to intersect be 1
At the vertex (vertices) of " V " word of passage, two semicircle spacing distances are " V " type passage（2）The 1/2 of width.
5. a kind of drug screening biochip with air chamber according to claim 1, it is characterised in that：The bottom sheet
（20）The passage of processing, it is all a height of 800 μm -1000 μm, a width of 300 μm -600 μm.
6. a kind of drug screening biochip with air chamber according to claim 1, it is characterised in that：Biochip point
Be 3 layers, most last layer be upper piece（19）, be sampling device and gas passage, including, gas access（3）, air chamber passage（4）, it is logical
Air flue（8）, air chamber（9）, gas vent（14）, and insertion upper piece（19）With middle（20）" V " type injection port one（1）, " V " type
Injection port two（17）, outlet（12）；Middle one layer is bottom sheet（20）, predominantly liquid communication pipeline, including " V " type passage（2）、
Turbine structure（5）, straight channel（18）, curved channel one（6）, cultivation region（7）, curved channel two（15）, waste liquid pool（10）、“U”
Type structure（16）；Bottom is chip base（13）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621278792.XU CN206266579U (en) | 2016-11-27 | 2016-11-27 | Drug screening biochip with air chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621278792.XU CN206266579U (en) | 2016-11-27 | 2016-11-27 | Drug screening biochip with air chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206266579U true CN206266579U (en) | 2017-06-20 |
Family
ID=59042721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621278792.XU Withdrawn - After Issue CN206266579U (en) | 2016-11-27 | 2016-11-27 | Drug screening biochip with air chamber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206266579U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754241A (en) * | 2016-11-27 | 2017-05-31 | 重庆科技学院 | A kind of application method of the drug screening biochip with air chamber |
| CN106350440B (en) * | 2016-11-27 | 2018-06-26 | 重庆科技学院 | A kind of drug screening biochip with gas chamber |
-
2016
- 2016-11-27 CN CN201621278792.XU patent/CN206266579U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754241A (en) * | 2016-11-27 | 2017-05-31 | 重庆科技学院 | A kind of application method of the drug screening biochip with air chamber |
| CN106350440B (en) * | 2016-11-27 | 2018-06-26 | 重庆科技学院 | A kind of drug screening biochip with gas chamber |
| CN106754241B (en) * | 2016-11-27 | 2018-10-23 | 重庆科技学院 | A kind of application method of the drug screening biochip with gas chamber |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170620 Effective date of abandoning: 20180626 |