CN206433636U - A kind of liquid nitrogen incubation chamber - Google Patents
A kind of liquid nitrogen incubation chamber Download PDFInfo
- Publication number
- CN206433636U CN206433636U CN201720087275.2U CN201720087275U CN206433636U CN 206433636 U CN206433636 U CN 206433636U CN 201720087275 U CN201720087275 U CN 201720087275U CN 206433636 U CN206433636 U CN 206433636U
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- China
- Prior art keywords
- liquid nitrogen
- box body
- lid
- incubation chamber
- handle
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- Expired - Fee Related
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- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of liquid nitrogen incubation chamber.The liquid nitrogen incubation chamber includes the lid and box body being made up of low temperature resistant light transmissive material；The inner side and outer side of the box body bottom is by white frosted processing；The lid internal diameter ＞ box body external diameters；Lid handle is provided with the top of the lid；The box body both sides are provided with box body handle.When storing liquid nitrogen and sample in box body, during the light of surrounding can enter liquid nitrogen from the top of incubation chamber and surrounding, and diffusing reflection occurs for the box body bottom handled in white frosted, to increase the field luminance in liquid nitrogen.The liquid nitrogen insulation box structure is simple, with low cost, easy to use, can be during glass freezing thaws, the sample in liquid nitrogen can conveniently be observed and various operations are carried out, so as at utmost improve operating efficiency and reduce the risk that sample surprisingly thaws.
Description
Technical field
The utility model belongs to Bioexperiment apparatus field, and in particular to a kind of liquid nitrogen incubation chamber.
Background technology
According to the World Health Organization（WHO）The 10-15% couple at child-bearing age is perplexed by infertility in estimation, developed country.In development
In country（Including China）, the women of child-bearing age for having 1.86 hundred million to 2002 suffer from infertility.The statistics of 2014 shows,
The incidence of China's reproductive population infertility is 12.5%, and infertility number is more than 40,000,000.Vitro fertilization-embryo implanting（in-
Vitro fertilization and embryo transfer, IVF-ET）Technology is the important hand of current treatment infertility
Section.From 1978 since the first " test-tube baby " in the world is born in Britain, current developed country helps pregnant birth by IVF-ET
Baby accounted for natus sum 1-3%, estimate whole world test-tube baby births more than 5,000,000 people.The mankind match somebody with somebody
Son（Sperm and egg mother cell）And the freezen protective of embryo, it is also an important IVF-ET deriving technology.Mankind's gamete and
Embryo be freezen protective in -196 DEG C of liquid nitrogen, now their metabolic activity close to stop, can preserving for a long time.From 1981
Since year first case freezen protective test-tube baby success, thousands of mankind's freezings have been had been set up from all parts of the world at present
Cryopreserved embryo, sperm bank and egg mother cell storehouse, the Number of infants being born by the freezen protective technology of embryo of silkworms and embryo also above
1,000,000 people.The method of mankind's gamete and Embryo freezing preservation includes slow freezing and glass freezing.Glass freezing be by
Sample is directly immersed in liquid nitrogen, so as to obtain ultrafast freezing rate, sample is directly reached glassy state.Because it is simple,
Quickly, efficiently, it is the freezing and storing method of current most main flow, is especially adapted for use in freezen protective egg mother cell and embryo.
The operation of cold glass freezing or defrosting needs to complete in superclean bench, due to the weight and volume of liquid nitrogen container
It is larger, it has not been convenient to mobile, therefore during freezing or defrosting, be required to use portable liquid nitrogen incubation chamber, directly will
Sample is put into or taken out rapidly liquid nitrogen.In addition, the terminal that liquid nitrogen incubation chamber can also be stored as sample.In refrigerating process
In, the frozen samples of some batches are deposited temporarily in liquid nitrogen incubation chamber, long-term storage in liquid nitrogen container is then uniformly transferred to；
The process of defrosting is transferred in incubation chamber and deposited temporarily then on the contrary, the frozen samples of some batches are taken out from liquid nitrogen container.
It can so avoid picking and placeing sample repeatedly and repeatedly opening liquid nitrogen container, cause liquid nitrogen to consume excessively and to other in liquid nitrogen container
Sample causes temperature fluctuation.
At present, do not have special liquid nitrogen incubation chamber in IVF laboratories, the most frequently used substitute includes：Foam box, insulation
Cup or heat-preserving container.But these incubation chambers have the shortcomings that one it is common, be to be made by lighttight material.When these
When filling with liquid nitrogen in attemperator, light is merely capable of being irradiated into the sample surfaces of internal storage by the upper surface of liquid nitrogen,
Same operator can only also observe the sample inside liquid nitrogen by the upper surface of liquid nitrogen；At ambient temperature, -196 DEG C of liquid
Nitrogen is also constantly in a kind of state close to boiling, and liquid nitrogen surface aggregation the vapor of condensation.Therefore, inside liquid nitrogen
The visual field is poor, and the sample in observation and operation liquid nitrogen is more difficult.During freeze-thaw, certain operations must be in liquid nitrogen
Complete, the patient information that for example cryovial is loaded or taken out on sleeve pipe and verification cryovial.Because the sample of glass freezing
Product volume is less than 1 microlitre, and cryovial once leaves liquid nitrogen, temperature can rapid rewarming, and then sample is produced freezing injury, even
Disintegrate.
Therefore, a kind of liquid nitrogen incubation chamber how is made, interim Cord blood can be carried out to sample, while can facilitate
The sample in liquid nitrogen is observed and operated, be most of to need to carry out mankind's gamete and embryo vitrifying freeze preserves skill
The laboratory urgent need to solve the problem in vitro fertilization of art.
Utility model content
In order to during glass freezing, interim Cord blood be carried out to sample, while can be convenient
Sample in liquid nitrogen is observed and operated, the utility model aims to provide a kind of liquid nitrogen incubation chamber, the incubation chamber can have
Effect ground transmits light in liquid nitrogen, operator is conveniently observed the sample in liquid nitrogen and carries out various operations, so that
Significant reduction operation difficulty, improves operating efficiency, and the risk that at utmost reduction sample surprisingly thaws.
To achieve these goals, the technical scheme that the utility model is used is：
The liquid nitrogen incubation chamber includes the lid and box body being made up of low temperature resistant light transmissive material；The inner side of the box body bottom
With outside by white frosted processing；The lid internal diameter ＞ box body external diameters；Lid handle is provided with the top of the lid；It is described
Box body both sides are provided with box body handle.
Wherein, the lid handle is the handle of convex type.The box body handle is the handle of invaginating.
The utility model is described in further detail below：
Liquid nitrogen incubation chamber described in the utility model includes the lid and box body being made up of low temperature resistant light transmissive material；The box body
Sample for depositing liquid nitrogen and freeze-thaw, the lid is used for the volatilization for reducing liquid nitrogen.When in box body store liquid nitrogen and
During sample, during the light of surrounding can enter liquid nitrogen from the top of incubation chamber and surrounding.Because incubation chamber bottom is unglazed
Line is directly transmitted into, therefore carries out white frosted processing in cassette bottom inner surface, increases the light in liquid nitrogen in the unrestrained anti-of bottom
Penetrate, improve field luminance.In operation, light source can be increased around incubation chamber（Such as desk lamp）, to increase illumination
Brightness.When the sample in liquid nitrogen is observed, from being carried out from the transparent side of incubation chamber is crossed, to avoid incubation chamber
The interference of the vapor of upper surface aggregation and the liquid nitrogen of boiling to sight, so that easily in liquid nitrogen under the clearly visual field
Sample carries out various operations.There is handle incubation chamber side, for carrying incubation chamber.Lid can prevent the excessive volatilization of liquid nitrogen,
And prevent that liquid nitrogen is spilt in handling process.Incubation chamber bottom surface outer surface also carries out frosted processing, increase incubation chamber bottom with
The frictional force of table top, prevents it from sliding.
Preferably, the lid, box body and handle, are made using the plastics disposal molding of different kind organism level of security,
Such as polypropylene, polystyrene or polycarbonate plastic.These materials are firm nontoxic, extremely efficient in preserving heat, and with good printing opacity
Property.
Preferably, lid internal diameter is slightly larger than box body external diameter, the liquid nitrogen for discharging volatilization, and pressure mistake is avoided in incubation chamber
Greatly, handle is used to open and close lid at the top of lid.
Preferably, the handle of incubation chamber both sides is invaginating groove, and carries out frosted processing, to increase incubation chamber carrying
During security.
Preferably, the box body highly be 250-300mm, length is 350-400mm, and width is 250-300mm, sideways with
The thickness of bottom surface is 40-60mm；The lid height 30-50mm, length is 360-410mm, and width is 260-310mm, side
The thickness of face and top surface is 2-4mm；The handle length 40-50mm of invaginating, width 20-30mm, depth 20-30mm.
Compared with prior art, the beneficial effects of the utility model are：During glass freezing, the incubation chamber can
Effectively to transmit light in liquid nitrogen, it can conveniently observe the sample in liquid nitrogen and carry out various operations, so that
The risk that at utmost reduction sample surprisingly thaws.Its is simple in construction, easy to use, with low cost, can significantly improve glass
The efficiency of glass freeze-thaw.
The utility model needs to carry out glass freezing defrosting except suitable for laboratory in vitro fertilization, being also applied for other
Cell biology, embryology and the Experiment of Zoology room of operation.
Brief description of the drawings
Fig. 1 is the utility model structural representation；
Fig. 2 is Figure 1A-A sectional view.
In figure：1st, lid；2nd, box body；3rd, box body handle；4th, lid handle.
Embodiment
Referring to Fig. 1 and Fig. 2, the liquid nitrogen incubation chamber includes the lid 1 and box body 2 being made up of low temperature resistant light transmissive material；Institute
The inner side and outer side of the bottom of box body 2 is stated by white frosted processing；The external diameter of 1 internal diameter ＞ box bodys of lid 2；The lid 1
Top is provided with lid handle 4；The both sides of box body 2 are provided with box body handle 3.
Wherein, the lid handle 4 is the handle of convex type.The box body handle 3 is the handle of invaginating.
Claims (3)
1. a kind of liquid nitrogen incubation chamber, it is characterised in that the liquid nitrogen incubation chamber includes the lid being made up of low temperature resistant light transmissive material
（1）And box body（2）；The box body（2）The inner side and outer side of bottom is by white frosted processing；The lid（1）Internal diameter ＞
Box body（2）External diameter；The lid（1）Top is provided with lid handle（4）；The box body（2）Both sides are provided with box body handle（3）.
2. liquid nitrogen incubation chamber as claimed in claim 1, it is characterised in that the lid handle（4）For the handle of convex type.
3. liquid nitrogen incubation chamber as claimed in claim 1, it is characterised in that the box body handle（3）For the handle of invaginating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720087275.2U CN206433636U (en) | 2017-01-23 | 2017-01-23 | A kind of liquid nitrogen incubation chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720087275.2U CN206433636U (en) | 2017-01-23 | 2017-01-23 | A kind of liquid nitrogen incubation chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206433636U true CN206433636U (en) | 2017-08-25 |
Family
ID=59635381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720087275.2U Expired - Fee Related CN206433636U (en) | 2017-01-23 | 2017-01-23 | A kind of liquid nitrogen incubation chamber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206433636U (en) |
-
2017
- 2017-01-23 CN CN201720087275.2U patent/CN206433636U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170825 Termination date: 20200123 |