CN201148434Y - Biological sample glassivation refrigeration and preservation tools - Google Patents
Biological sample glassivation refrigeration and preservation tools Download PDFInfo
- Publication number
- CN201148434Y CN201148434Y CN 200720131855 CN200720131855U CN201148434Y CN 201148434 Y CN201148434 Y CN 201148434Y CN 200720131855 CN200720131855 CN 200720131855 CN 200720131855 U CN200720131855 U CN 200720131855U CN 201148434 Y CN201148434 Y CN 201148434Y
- Authority
- CN
- China
- Prior art keywords
- straw
- biological sample
- thin
- length
- protecting pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012472 biological sample Substances 0.000 title claims abstract description 20
- 238000004321 preservation Methods 0.000 title description 5
- 238000005057 refrigeration Methods 0.000 title description 2
- 239000010902 straw Substances 0.000 claims abstract description 31
- 238000007710 freezing Methods 0.000 claims abstract description 26
- 230000008014 freezing Effects 0.000 claims abstract description 26
- 239000011521 glass Substances 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 11
- 239000007769 metal material Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 22
- 210000004027 cell Anatomy 0.000 abstract description 15
- 239000007788 liquid Substances 0.000 abstract description 14
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 11
- 238000001816 cooling Methods 0.000 abstract description 4
- 238000011109 contamination Methods 0.000 abstract description 3
- 210000002257 embryonic structure Anatomy 0.000 abstract 2
- 210000004602 germ cell Anatomy 0.000 abstract 2
- 238000004017 vitrification Methods 0.000 abstract 1
- 102000002322 Egg Proteins Human genes 0.000 description 11
- 108010000912 Egg Proteins Proteins 0.000 description 11
- 210000004681 ovum Anatomy 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 8
- 210000001161 mammalian embryo Anatomy 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 5
- 239000004743 Polypropylene Substances 0.000 description 3
- -1 polypropylene Polymers 0.000 description 3
- 229920001155 polypropylene Polymers 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000035606 childbirth Effects 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model relates to a vitrified cooling and storage tool of biological samples such as germ cells, embryos and so on. The tool rapidly freezes the biological samples and the samples can not be directly exposed in liquid nitrogen, thereby preventing the potential risk of biological contamination. The vitrified cooling and storage tool comprises a straw and a sheath pipe; the sheath pipe is sleeved on the straw; the middle part of the straw is a fine thin-walled pipe that is 20mm to 30mm long; the diameter phi of the fine thin-walled pipe is 0.3mm to 0.35mm; two sides of the middle part of the sheath pipe are symmetrically provided with an internally concave window. The tool with the straw can be used for vitrified freezing and storage of germ cells, embryos and other cells; thus the biological samples can be frozen quickly through vitrification, but can not be directly exposed in the liquid nitrogen so as to prevent the potential risk of biological contamination.
Description
Technical field
The utility model relates to employed instrument in the glass freezing that is applied to ovum and embryo and other cells and the preservation process.
Background technology
Human ovum and embryo's Refrigeration Technique is a strong treatment and fertility store method in auxiliary procreation technology.Especially the freezing preservation of ovum is for because pelvic conditions, and the dangerous women of the ovarian function that side effect produced of radiotherapy and chemotherapy forfeiture is a kind of practicable fertility store method.Although the report that uses the conceived childbirth of human freezing ovum has been arranged, the freezing preservation technology of human ovum still is in developmental stage.The glass freezing technology can make the survival rate raising after human oocytes thaws and obtain effective gestation and live baby's birth at present.
The glass freezing technology of cell promptly makes the moisture in the viable cell not have ice crystal formation and directly become the hyaloid curdled appearance in the refrigerative process.Theoretically, improving chilling rate will cause intracellular moisture to form the vitrifying state.It is to guarantee that ICW forms the vitrifying state that but only actually improves chilling rate.According to studies show that, most of glass freezing technology are all used the very cryoprotectant of high density, for example, and dimethyl sulfoxide (DMSO) (DMSO) or glycerine or the like.Could guarantee the frozen cell post-thaw survival like this.Up to the present the cell glass freezing technology that is adopted is cell directly to be exposed to liquid nitrogen (196 ℃) make intracellular moisture vitrifying to reach fast cooling.Yet cell sample directly is exposed to liquid nitrogen will be had by the danger of the virus and bacteria in the liquid nitrogen and other pathogen contamination.If cell is put into test tube or other containers of sealing, carry out glass freezing again, certainly will not reach the speed purpose of the fast cooling of expection.Given this, improving chilling rate is to overcome cell glass freezing technology and biological sample safe storage problem demanding prompt solution in liquid nitrogen with directly not exposing biological sample.
The utility model content
Both can make biological sample freezing in order to solve with rapid glassization, can make biological sample directly not be exposed to liquid nitrogen to prevent the problem of potential biological pollution danger again, the utility model provides biological sample glass freezing and conserving appliances such as a kind of ovum and embryo.
Concrete structural design scheme is as follows:
Biological sample glass freezing and conserving appliance comprise straw and protecting pipe, and the straw two ends can be heated or plug for seal, and protecting pipe is set in the straw middle part.
The length of described straw is 80-90mm, and the two ends diameter is Φ 1.5-2.0mm, and its middle part is very thin thin-walled tube, and the very thin thin-walled tube length at middle part is 20-30mm, and diameter is Φ 0.3-0.35mm;
Described protecting pipe length is 45-50mm, and diameter is Φ 2.05-2.55mm; It is that 30-35mm, width are 1.0-1.5mm indent window that its middle part zygomorphy is provided with length.
The material of described straw is a plastic material, as polypropylene;
The material of described protecting pipe is a metallic substance.
Useful technique effect of the present utility model is embodied in following several aspect:
1, the utility model instrument is used for the glass freezing preservation of ovum or embryo and other cells, both can make biological sample freezing with rapid glassization, can make biological sample directly not be exposed to liquid nitrogen to prevent potential biological pollution danger again.
2, the both sides of the utility model protecting pipe have the indent window, can conveniently the straw instrument directly be placed and examine under a microscope.
3, the utility model partly is positioned at central authorities because of very thin thin-walled tube, and both ends open place diameter is bigger, is convenient to draw ovum and embryo and other cells, remedies the shortcoming that like product is drawn the sample difficulty.
4, the straw of the utility model use is dainty, has avoided the bigger shortcoming of like product volume, and it is little to take up room, and makes that storage quantity is many, reduces liquid nitrogen loss effectively, reduces freezing cost.
5, the utility model has used the metal covering pipe, has protected straw effectively, makes straw be not easy to be fractureed, damage, and has certain flintiness.
Description of drawings
Fig. 1 is the utility model structural representation,
Fig. 2 is Fig. 1 vertical view,
Fig. 3 is for protecting the bassoon structural representation,
Fig. 4 is the straw structural representation,
Fig. 5 is the vertical view of Fig. 3,
Fig. 6 is the side-view of Fig. 3.
Embodiment
Below in conjunction with accompanying drawing, the utility model is further described by embodiment.
Embodiment 1:
Biological sample glass freezing and conserving appliance comprise straw 1 and protecting pipe 2, and protecting pipe 2 is sleeved on straw 1 middle part, sees Fig. 1 and Fig. 2;
The length of straw is 90mm, and the two ends diameter is Φ 2.0mm, and its middle part is very thin thin-walled tube, and the very thin thin-walled tube length at middle part is 30mm, and diameter is Φ 0.35mm, sees Fig. 4.The material of straw is a polypropylene.
Protecting pipe length is 50mm, and diameter is Φ 2.55mm; It is that 35mm, width are 1.5mm indent window that its middle part zygomorphy is provided with length, sees Fig. 3, Fig. 5 and Fig. 6.The material of protecting pipe is a metallic substance.
The first-selected glass freezing of ovum or embryo and cell and the schedule of operation of thawing are as follows.Ovum or embryo and cell changed in the glass freezing liquid after suspending in balance liquid 5 minutes.In second ovum or embryo and cell and glass freezing liquid are sucked very thin thin-walled tube 3 parts of the carrying biological sample of straw 1 at 45-60, then with straw 1 two ends with heating or sealing member plug for seal, and drop in the liquid nitrogen fast and preserve.When thawing, the straw 1 that is loaded with sample is directly put into 37 ℃ of warm water and is thawed.Cut off straw 1 two ends sample is released, thereafter sample is put into thawing solution and diluent.
The first-selected low-expansion material of straw material is to prevent assembly explosion during fast and big like this temperature variation between room temperature and liquid nitrogen temperature.Straw partly is fit to make (add filling agent and reinforcer and can improve estimated performance, first-selected material is Profax PD626-H12 Basell) with polypropylene, and protecting jacket can be made of stainless steel.
Embodiment 2:
Biological sample glass freezing and conserving appliance comprise straw 1 and protecting pipe 2, and protecting pipe 2 is sleeved on straw 1 middle part;
The length of straw 1 is 80mm, and the two ends diameter is Φ 1.5mm, and its middle part is very thin thin-walled tube 3, and the very thin thin-walled tube length at middle part is 20mm, and diameter is Φ 0.3mm; Very thin thin-walled tube is partly for carrying the bearing part of biological sample;
Protecting pipe 2 length are 45mm, and diameter is Φ 2.05mm; It is that 30mm, width are 1.0 indent windows 4 that its middle part zygomorphy is provided with length.
Other is with embodiment 1.
Claims (3)
1, biological sample glass freezing and conserving appliance is characterized in that: comprise straw and protecting pipe, protecting pipe is set in the straw middle part,
The length of described straw is 80-90mm, and the two ends diameter is Φ 1.5-2.0mm, and its middle part is very thin thin-walled tube, and the very thin thin-walled tube length at middle part is 20-30mm, and diameter is Φ 0.3-0.35mm;
Described protecting pipe length is 45-50mm, and diameter is Φ 2.05-2.55mm; It is that 30-35mm, width are 1.0-1.5mm indent window that its middle part zygomorphy is provided with length.
2, biological sample glass freezing according to claim 1 and conserving appliance is characterized in that: the material of described straw is a plastic material.
3, biological sample glass freezing according to claim 1 and conserving appliance is characterized in that: the material of described protecting pipe is a metallic substance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720131855 CN201148434Y (en) | 2007-12-21 | 2007-12-21 | Biological sample glassivation refrigeration and preservation tools |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720131855 CN201148434Y (en) | 2007-12-21 | 2007-12-21 | Biological sample glassivation refrigeration and preservation tools |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201148434Y true CN201148434Y (en) | 2008-11-12 |
Family
ID=40116201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200720131855 Expired - Lifetime CN201148434Y (en) | 2007-12-21 | 2007-12-21 | Biological sample glassivation refrigeration and preservation tools |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201148434Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105163579A (en) * | 2013-04-09 | 2015-12-16 | 楼伟 | Biological sample vitrification carrier and usage thereof |
| CN105705626A (en) * | 2013-10-29 | 2016-06-22 | 学校法人北里研究所 | Tool for vitrifying cryopreservation of cells or tissue |
| CN109644993A (en) * | 2019-01-31 | 2019-04-19 | 力盟低温医学(深圳)有限公司 | A kind of method and utensil saving glass freezing embryo in -80 degree refrigerators |
-
2007
- 2007-12-21 CN CN 200720131855 patent/CN201148434Y/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105163579A (en) * | 2013-04-09 | 2015-12-16 | 楼伟 | Biological sample vitrification carrier and usage thereof |
| CN105705626A (en) * | 2013-10-29 | 2016-06-22 | 学校法人北里研究所 | Tool for vitrifying cryopreservation of cells or tissue |
| CN105705626B (en) * | 2013-10-29 | 2018-11-16 | 学校法人北里研究所 | The vitrificated cryopreserration tool of cell or tissue |
| CN109644993A (en) * | 2019-01-31 | 2019-04-19 | 力盟低温医学(深圳)有限公司 | A kind of method and utensil saving glass freezing embryo in -80 degree refrigerators |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20081112 Effective date of abandoning: 20071221 |