CN206396225U - A kind of real-time fluorescence quantitative PCR instrument - Google Patents
A kind of real-time fluorescence quantitative PCR instrument Download PDFInfo
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- CN206396225U CN206396225U CN201720036814.XU CN201720036814U CN206396225U CN 206396225 U CN206396225 U CN 206396225U CN 201720036814 U CN201720036814 U CN 201720036814U CN 206396225 U CN206396225 U CN 206396225U
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- pcr
- column spinner
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- ice bucket
- casing
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Abstract
The utility model is a kind of real-time fluorescence quantitative PCR instrument, the lower surface center of protecting cover is provided with the swivel head connected with external power source, fluorescence detection device is located on the inwall of casing, the top center of column spinner is provided with the neck supporting with swivel head, ice bucket is detachably secured to the bottom part down of column spinner, many heating rods have been extended radially from column spinner, PCR plate is enclosed on column spinner, PCR plate can bottom-up be slided along column spinner, the tapered PCR pipe in bottom is inserted with each pore of PCR plate, the utility model is provided with the ice bucket that can be connected with the dismounting of rotation column bottom, so that PCR pipe is either when adding medicine and reagent, or when during in the casing for be transferred to PCR instrument, the bottom of PCR pipe can be made to keep relatively low temperature environment, make medicine not mutability, and, in experiment, ice bucket is removed, open the heating rod of varying number, it is i.e. enough to provide different temperature environments for experiment.
Description
Technical field
The utility model is related to PCR instrument field, more particularly to a kind of real-time fluorescence quantitative PCR instrument.
Background technology
PCR, i.e. PCR, are a kind of Protocols in Molecular Biologies for the specific DNA fragmentation of amplification in vitro,
Micro DNA can be significantly increased by replicating, actual PCR instrument is exactly a temperature control device, can be in denaturation temperature, renaturation temperature
Control is adjusted well between degree and elongating temperature.Wherein quantitative real time PCR Instrument is to add fluorescence when PCR is expanded
Group is marked, and the result of amplification is gathered in real time by fluorescence signal acquisition system, is mainly used in clinical medicine detection, biological
Medicine is researched and developed, food service industry, the mechanism such as research institutions.
Medicine needed for PCR needs to be temporarily placed in ice chest typically in Cord blood when taking-up is used, and then will
Medicine is picked up to be loaded to PCR pipe, with i.e. medicine is put back into ice chest.But PCR pipe is placed in PCR plate, in dosing
The temperature environment of PCR pipe is difficult to ensure that during product, this process easily causes PCR pipe degradation of substrates, medicine is denatured, and then
Influence experimental result.
Utility model content
The utility model aims to solve the problem that the deficiencies in the prior art, and provides a kind of real-time fluorescence quantitative PCR instrument.
The utility model to achieve the above object, using following technical scheme：A kind of real-time fluorescence quantitative PCR instrument, it is special
Levy and be, including casing, protecting cover, fluorescence detection device, ice bucket and sampling device, the protecting cover is located at the top of the casing,
The lower surface center of the protecting cover is provided with the swivel head connected with external power source, and the fluorescence detection device is located at the casing
On inwall, the fluorescence detection device is connected by wire with power supply,
The sampling device is respectively positioned on inside the casing, and the sampling device includes column spinner, PCR plate and bottom plate, institute
The top center for stating column spinner is provided with the neck supporting with the swivel head, described when the protecting cover is fastened on the casing
Swivel head is fixed in the neck, and the column spinner can rotate under the drive of the swivel head,
The bottom centre of the column spinner is provided with screwed hole, and the center of the ice bucket has protruded upward stud, by described
The ice bucket is detachably secured to the bottom part down of the column spinner by the connection of stud and the screwed hole,
The bottom plate is located at the bottom of the column spinner, and the bottom surface of the bottom surface of the bottom plate and the column spinner is positioned at same
Plane, has extended radially many heating rods from the column spinner, and the heating rod is connected with external power source, the PCR
Sleeve-board is on the column spinner and above the heating rod, and the PCR plate can bottom-up be slided along the column spinner
It is dynamic,
The PCR plate and the bottom plate, which are provided with multiple penetrating pores, each pore of the PCR plate, to be inserted
There is the PCR pipe that bottom is tapered, the PCR plate lower surface is exposed in the bottom of the PCR pipe, and works as the PCR plate along the rotation
When rotary column slides into bottom, the bottom for the PCR pipe being inserted with the PCR plate passes through the bottom plate and is immersed in the ice bucket,
The middle part of the column spinner is provided with bayonet socket, and the PCR plate can be fixed at the bayonet socket of the column spinner, now institute
The bottom for stating PCR pipe is located above the heating rod.
Particularly, the hole slot corresponding with the pore is provided with the ice bucket, and works as the PCR plate along the column spinner
When sliding into bottom, the bottom for the PCR pipe being inserted with the PCR plate passes through the bottom plate and is immersed in the hole slot of the ice bucket.
Particularly, the every heating rod can be switched individually.
The beneficial effects of the utility model are：The utility model is provided with the ice bucket that can be connected with the dismounting of rotation column bottom,
So that PCR pipe is either when adding medicine and reagent, or in the casing for be transferred to PCR instrument during when, PCR can be made
The bottom of pipe keeps relatively low temperature environment, makes medicine not mutability, also, in experiment, ice bucket is removed, and opens different numbers
The heating rod of amount is that is, enough to provide different temperature environments for experiment.
Brief description of the drawings
Fig. 1 is casing structure schematic diagram of the present utility model；
Fig. 2 is structural representation when PCR plate of the present utility model is located at rotation column bottom；
Fig. 3 is structural representation when PCR plate of the present utility model is located at the bayonet socket of column spinner；
Fig. 4 is the structural representation of ice bucket of the present utility model；
Fig. 5 is the polycrystalline substance schematic diagram of column spinner of the present utility model；
In figure：1- casings；2- protecting covers；3- swivel heads；4- fluorescence detection devices；5- column spinners；6- necks；7-PCR plates；8-
Pore；9- heating rods；10- bottom plates；11- ice buckets；12-PCR is managed；13- studs；14- screwed holes；
It is described in detail referring to the drawings below with reference to embodiment of the present utility model.
Embodiment
The utility model is described in further detail with reference to the accompanying drawings and examples：
As shown in Fig. 1,2,3,4,5, including casing 1, protecting cover 2, fluorescence detection device 4, ice bucket 11 and sampling device, it is described
Protecting cover 2 is located at the top of the casing 1, and the lower surface center of the protecting cover 2 is provided with the swivel head 3 connected with external power source, institute
Fluorescence detection device 4 is stated on the inwall of the casing 1, the fluorescence detection device 4 is connected by wire with power supply,
The sampling device is respectively positioned on inside the casing 1, and the sampling device includes column spinner 5, PCR plate 7 and bottom plate
10, the top center of the column spinner 5 is provided with the neck 6 supporting with the swivel head 3, and the protecting cover 2 is fastened on the casing 1
When upper, the swivel head 3 is fixed in the neck 6, and the column spinner 5 can rotate under the drive of the swivel head 3,
The bottom centre of the column spinner 5 is provided with screwed hole 14, and the center of the ice bucket 11 has protruded upward stud 13, leads to
The ice bucket 11 is detachably secured under the bottom of the column spinner 5 by the connection for crossing the stud 13 and the screwed hole 14
Side,
The bottom plate 10 is located at the bottom of the column spinner 5, the bottom surface of the bottom plate 10 and the bottom surface position of the column spinner 5
In same plane, many heating rods 9 are extended radially from the column spinner 5, the heating rod 9 is connected with external power source
Connect, the PCR plate 7 is enclosed on the column spinner 5 and positioned at the top of heating rod 9, the PCR plate 7 can be along the rotation
Post 5 is slided bottom-up,
The PCR plate 7 and the bottom plate 10 are provided with multiple penetrating pores 8, each pore 8 of the PCR plate 7
In be inserted with the tapered PCR pipe 12 in bottom, the lower surface of PCR plate 7 is exposed in the bottom of the PCR pipe 12, and as the PCR
Plate 7 along the column spinner 5 slide into bottom when, the bottom of the PCR pipe 12 being inserted with the PCR plate 7 through the bottom plate 10 and
It is immersed in the ice bucket 11,
The middle part of the column spinner 5 is provided with bayonet socket, and the PCR plate 7 can be fixed at the bayonet socket of the column spinner 5, this
The bottom of Shi Suoshu PCR pipes 12 is located at the top of heating rod 9.
Particularly, the hole slot corresponding with the pore 8 is provided with the ice bucket 11, and works as the PCR plate 7 along the rotation
When rotary column 5 slides into bottom, the bottom for the PCR pipe 12 being inserted with the PCR plate 7 passes through the bottom plate 10 and is immersed in the ice bucket
In 8 hole slot.
Particularly, the every heating rod 9 can be switched individually.
During utility model works, ice bucket 11 is fixed on to the bottom of column spinner 5 first, then by the slide downward of PCR plate 7
To being contacted with bottom plate 10, PCR pipe 12 is inserted in pore 8 successively, now, the bottom of PCR pipe 8 passes through bottom plate 10 and is immersed in ice
In bucket 11, you can to reagent adding medicine in PCR pipe 8, it is ensured that the medicine in PCR pipe 8 is in the cold storage environment of ice bucket 11.
When needing to carry out experimental implementation, at the bayonet socket of the upward sliding of PCR plate 7 to column spinner 5, by the bottom of column spinner 5
Ice bucket 11 is removed, and whole sampling device is placed in casing 1, closes protecting cover 2, the swivel head 3 on protecting cover 2 is fixed on rotation
In the neck 6 at the top of post 5, heating rod 9 is opened as needed, machine is opened, then swivel head 3 drives PCR plate 7 and PCR pipe 12 to enter
Row rotates at a high speed, while heating rod 9 also rotates under the drive of column spinner 5, equably distributes heat in casing 1 from the bottom to top
In, while being detected by fluorescence detection device 4 to the reagent in PCR pipe 12.
The utility model is exemplarily described above in conjunction with accompanying drawing, it is clear that the utility model implement not by
The limitation of aforesaid way, if the various improvement that method design of the present utility model and technical scheme are carried out are employed, or without
Improvement directly applies to other occasions, within protection domain of the present utility model.
Claims (3)
1. a kind of real-time fluorescence quantitative PCR instrument, it is characterised in that including casing (1), protecting cover (2), fluorescence detection device (4), ice
Bucket (11) and sampling device, the protecting cover (2) are located at the top of the casing (1), and the lower surface center of the protecting cover (2) is provided with
The swivel head (3) connected with external power source, the fluorescence detection device (4) is located on the inwall of the casing (1), the fluorescence
Detection means (4) is connected by wire with power supply,
The sampling device is respectively positioned on the casing (1) inside, and the sampling device includes column spinner (5), PCR plate (7) and bottom
Plate (10), the top center of the column spinner (5) is provided with the neck (6) supporting with the swivel head (3), protecting cover (2) button
When closing on the casing (1), the swivel head (3) is fixed in the neck (6), and the column spinner (5) can be in the rotation
Rotated under the drive of rotary head (3),
The bottom centre of the column spinner (5) is provided with screwed hole (14), and the center of the ice bucket (11) has protruded upward stud
(13) ice bucket (11), is detachably secured to by the rotation by the connection of the stud (13) and the screwed hole (14)
The bottom part down of rotary column (5),
The bottom plate (10) is located at the bottom of the column spinner (5), the bottom surface of the bottom plate (10) and the bottom of the column spinner (5)
Face is generally aligned in the same plane, and many heating rods (9), the heating rod (9) and outside have been extended radially from the column spinner (5)
Power supply is connected, and the PCR plate (7) is enclosed on the column spinner (5) and above the heating rod (9), the PCR plate
(7) can bottom-up it be slided along the column spinner (5),
The PCR plate (7) and the bottom plate (10) are provided with multiple penetrating pores (8), the PCR plate (7) it is each described
The tapered PCR pipe in bottom (12) is inserted with pore (8), the PCR plate (7) following table is exposed in the bottom of the PCR pipe (12)
Face, and when the PCR plate (7) slides into bottom along the column spinner (5), the PCR pipe (12) being inserted with the PCR plate (7)
Bottom through the bottom plate (10) and being immersed in the ice bucket (11),
The middle part of the column spinner (5) is provided with bayonet socket, and the PCR plate (7) can be fixed at the bayonet socket of the column spinner (5),
Now the bottom of the PCR pipe (12) is located above the heating rod (9).
2. a kind of real-time fluorescence quantitative PCR instrument according to claim 1, it is characterised in that be provided with the ice bucket (11)
The hole slot corresponding with the pore (8), and when the PCR plate (7) slides into bottom along the column spinner (5), the PCR
The bottom for the PCR pipe (12) being inserted with plate (7) is through the bottom plate (10) and is immersed in the hole slot of the ice bucket (11).
3. a kind of real-time fluorescence quantitative PCR instrument according to claim 1, it is characterised in that every heating rod (9) energy
Enough individually switches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720036814.XU CN206396225U (en) | 2017-01-13 | 2017-01-13 | A kind of real-time fluorescence quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720036814.XU CN206396225U (en) | 2017-01-13 | 2017-01-13 | A kind of real-time fluorescence quantitative PCR instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206396225U true CN206396225U (en) | 2017-08-11 |
Family
ID=59513640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720036814.XU Active CN206396225U (en) | 2017-01-13 | 2017-01-13 | A kind of real-time fluorescence quantitative PCR instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206396225U (en) |
-
2017
- 2017-01-13 CN CN201720036814.XU patent/CN206396225U/en active Active
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