CN206378421U - Fluorescent quantitation instrument - Google Patents
Fluorescent quantitation instrument Download PDFInfo
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- CN206378421U CN206378421U CN201621345380.3U CN201621345380U CN206378421U CN 206378421 U CN206378421 U CN 206378421U CN 201621345380 U CN201621345380 U CN 201621345380U CN 206378421 U CN206378421 U CN 206378421U
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- China
- Prior art keywords
- optical fiber
- fluoroscopic examination
- fluorescent quantitation
- quantitation instrument
- temperature adjustment
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Abstract
The utility model discloses a kind of fluorescent quantitation instrument, it includes cabinet, and cabinet includes temperature adjustment base and the fluoroscopic examination portion being hinged with temperature adjustment base one end；Temperature adjustment base, which is provided with a sample portion, the jack that sample portion is used to place PCR reaction tubes provided with several, PCR reaction tubes, is filled with increased response agent, and the material in sample portion is aluminium alloy；Fluoroscopic examination portion, which is provided with, is provided with several optical fiber spy mouths being engaged with jack in a groove being engaged with sample portion, groove；Fluoroscopic examination portion includes LED/light source portion, exciter filter, dichroscope, fiber coupling lens, y-type optical fiber, focus lamp, detection optical filter and the photoelectric conversion module for being respectively used to launch blue light, feux rouges and white light, the common port of y-type optical fiber is provided with self-focusing optical fiber, and self-focusing optical fiber is located at optical fiber and visited at mouth；Photoelectric conversion module includes the channel-photomultiplier and signal compensation circuit being sequentially connected；The fluorescent quantitation instrument has higher sensitivity and precision.
Description
Technical field
The utility model is related to science ＆ research instrument, technical field of medical equipment, and in particular to a kind of fluorescent quantitation instrument.
Background technology
Quantitative fluorescent PCR (Real-time quantitative PCR, also referred to as quantitative PCR, referred to as QPCR) technology
Refer to add fluorophor in PCR reaction systems, whole PCR processes are monitored in real time using fluorescence signal accumulation, finally by
The method that standard curve carries out quantitative analysis to unknown template；However, there is detection in the fluorescent quantitation instrument in currently available technology
Sensitivity is weak, heating is slow and the low problem of accuracy of detection.
Utility model content
The purpose of this utility model is to provide a kind of sensitivity and the high fluorescent quantitation instrument of precision.
In order to solve the above technical problems, the utility model provides a kind of fluorescent quantitation instrument, it includes cabinet, and cabinet includes temperature
Degree adjusting base and the fluoroscopic examination portion being hinged with temperature adjustment base one end；Temperature adjustment base is provided with a sample portion,
Increased response agent, sample portion are filled with the jack that sample portion is used to place PCR reaction tubes provided with several, PCR reaction tubes
Material be aluminium alloy；Fluoroscopic examination portion, which is provided with a groove being engaged with sample portion, groove, is provided with several with inserting
The optical fiber that hole is engaged visits mouth；
Fluoroscopic examination portion include being respectively used to launching blue light, the LED/light source portion of feux rouges and white light, exciter filter, two to
Look mirror, fiber coupling lens, y-type optical fiber, focus lamp, detection optical filter and photoelectric conversion module, the common port of y-type optical fiber are provided with
Self-focusing optical fiber, self-focusing optical fiber is located at optical fiber and visited at mouth；Photoelectric conversion module includes the channel-type photomultiplier transit being sequentially connected
Pipe and signal compensation circuit.
Further, temperature adjustment base includes radiator, semiconductor thermally conductive sheet, the heat conduction heat set gradually from bottom to up
Manage and in the resistance wire of roundabout type, specimen locations are in the upper end of resistance wire；One end of heat-conductive thermo tube is connected with semiconductor thermally conductive sheet,
The other end is connected with radiator.
Further, increased response agent is one kind in glycerine, bovine serum albumin(BSA), formamide, spermidine and glycine betaine
Or it is a variety of.
Further, the lower end of cabinet is symmetrically arranged with sucker pin schematic diagram.
Further, fluoroscopic examination portion is provided with one groove.
Further, the bottom in fluoroscopic examination portion is provided with a sealing ring.
Further, focus lamp is prism.
The beneficial effects of the utility model are：
1st, one end of temperature adjustment base and fluoroscopic examination portion are hinged, when that need to be detected, overturn fluoroscopic examination
Portion, PCR reaction tubes are positioned in jack, and fluoroscopic examination portion is fastened in upset, make fluoroscopic examination portion and temperature adjustment base into
It is integrated, and then it is detected, its structure is novel, places facility.
2nd, increased response agent is filled with PCR reaction tubes, preferably increased response agent is glycerine, bovine serum albumin(BSA), formyl
One or more in amine, spermidine and glycine betaine, it can promote various hot resistant DNA polymerases to Various Complex structural DNA mould
Effective amplification of plate, the sensitivity and specificity of increase PCR reactions.
3rd, the material in sample portion is aluminium alloy, with good thermal conductivity, when entering performing PCR reaction, by temperature adjustment bottom
The heat that seat is produced is delivered in PCR reaction tubes more easily by sample portion, it is to avoid heat unnecessary loss.
4th, several optical fiber being engaged with jack are provided with groove and visit mouth, mouth is visited to being placed on corresponding insert by optical fiber
In hole PCR hair should pipe detected, one-to-one corresponding, being capable of the multiple samples of one-time detection.
5th, LED/light source portion is photochromic using blueness, red and white three, it is ensured that have in the range of whole spectral detection compared with
Strong light intensity.
6th, using channel-photomultiplier as electrooptical device, with higher sensitivity.
7th, the setting of signal compensation circuit, can reduce the generation of interference signal, improve accuracy of detection.
8th, in roundabout type resistance wire setting, the heating to sample portion can be accelerated.
9th, under the mating reaction of semiconductor thermally conductive sheet and resistance wire, its flexibility and applicability are improved, with temperature control
Scope is big, the advantages of error is small.
10th, the lower end of cabinet is symmetrically arranged with sucker pin schematic diagram, can firmly adhesive is on a surface of an by the casing, it is to avoid
When upset fastens fluoroscopic examination portion, rocking for casing is caused.
Brief description of the drawings
Fig. 1 is the structural representation of fluorescent quantitation instrument.
Fig. 2 is the structural representation of fluorescent quantitation instrument buckling state.
Fig. 3 is the structural representation in the fluoroscopic examination portion of fluorescent quantitation instrument.
Fig. 4 is the semiconductor thermally conductive sheet of fluorescent quantitation instrument and the structural representation of resistance wire.
Wherein：1st, cabinet；2nd, fluoroscopic examination portion；3rd, temperature adjustment base；4th, sample portion；5th, jack；6th, groove；7th, optical fiber
Visit mouth；8th, sealing ring；9th, groove；10th, sucker pin schematic diagram；11st, y-type optical fiber；12nd, self-focusing optical fiber；13rd, common port；14th, LED/light source
Portion；15th, exciter filter；16th, dichroscope；17th, fiber coupling lens；18th, focus lamp；19th, optical filter is detected；20th, passage
Formula photomultiplier；21st, signal compensation circuit；22nd, resistance wire；23rd, semiconductor thermally conductive sheet.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a kind of embodiment of the utility model, rather than whole realities
Apply example.Based on the embodiment in the utility model, those of ordinary skill in the art institute under the premise of creative work is not made
The every other embodiment obtained, belongs to protection domain of the present utility model.
As shown in Fig. 1 ~ Fig. 4, the fluorescent quantitation instrument includes cabinet 1, and cabinet 1 includes temperature adjustment base 3 and adjusted with temperature
The fluoroscopic examination portion 2 that section base 3 one end is hinged；Temperature adjustment base 3 is provided with a sample portion 4, and sample portion 4 is provided with some
The individual jack 5 for being used to place PCR reaction tubes；When that need to be detected, fluoroscopic examination portion 2 may be reversed, PCR reaction tubes are positioned over
In jack 5, fluoroscopic examination portion 2 is fastened in upset, is integrally formed fluoroscopic examination portion 2 and temperature adjustment base 3, and then enter to it
Row detection, its structure is novel, places facility.
Increased response agent is filled with PCR reaction tubes, the material in sample portion 4 is aluminium alloy；In specific implementation, preferably instead
It is the one or more in glycerine, bovine serum albumin(BSA), formamide, spermidine and glycine betaine to answer reinforcing agent, and it can promote various
Effective amplification of the hot resistant DNA polymerase to Various Complex structural DNA template, the sensitivity and specificity of increase PCR reactions.
Meanwhile, sample portion 4 uses aluminum alloy material, anti-entering performing PCR because aluminum alloy material has good thermal conductivity
At once, the heat produced by temperature adjustment base 3 is delivered in PCR reaction tubes more easily by sample portion 4, it is to avoid heat is not
Necessary loss.
Fluoroscopic examination portion 2 is provided with a groove 6 being engaged with sample portion 4, groove 6 and is provided with several and jack 5
The optical fiber that is engaged visits mouth 7, by optical fiber visit PCR hairs that 7 pairs of mouth is placed in corresponding jack 5 should pipe detect, one a pair
Should, being capable of the multiple samples of one-time detection；In specific implementation, preferably jack 5 is 96, and 96 samples can be examined simultaneously
Survey.
Fluoroscopic examination portion 2 include being respectively used to launching blue light, the LED/light source portion 14 of feux rouges or white light, exciter filter 15,
Dichroscope 16, fiber coupling lens 17, y-type optical fiber 11, focus lamp 18, detection optical filter 19 and photoelectric conversion module, and it is excellent
It is prism to select focus lamp 18；The common port 13 of y-type optical fiber 11 is provided with self-focusing optical fiber 12, and self-focusing optical fiber 12 is located at optical fiber and visits mouth
At 7；Photoelectric conversion module includes the channel-photomultiplier 20 and signal compensation circuit 21 being sequentially connected.
In specific implementation, the light sent by LED/light source portion 14 is filtered into exciting for respective wavelength through exciter filter 15
Light, then coupled by dichroscope 16 into fiber coupling lens 17, then the light after coupling is delivered to the one of y-type optical fiber 11
In individual branch；Now, after light reaches self-focusing optical fiber 12 through Y types optical fiber, then by optical fiber visit mouth 7 be irradiated to be placed on it is corresponding
PCR hairs in jack 5 should be in pipe, so that the response sample that corresponding PCR hairs should be in pipe sends fluorescence.
Then, fluorescence reaches the output end of Y type optical fiber through self-focusing optical fiber 12 again, and by the focussed collimated of focus lamp 18,
Filtered again by detection optical filter 19, filter out pure fluorescence, finally received and by fluorescence signal by photoelectric conversion module
It is converted into current signal output；It effectively reduces loss of the light in communication process, substantially increases the sensitive of fluoroscopic examination
Degree.
Wherein, LED/light source portion 14 is photochromic using blueness, red and white three, it is ensured that in the range of whole spectral detection
There is stronger light intensity；And using channel-photomultiplier 20 as electrooptical device, with higher sensitivity；Together
When, the setting of signal compensation circuit 21 can reduce the generation of interference signal, improve accuracy of detection.
The temperature adjustment base 3 of the fluorescent quantitation instrument includes radiator, the semiconductor thermally conductive sheet set gradually from bottom to up
23rd, heat-conductive thermo tube and the resistance wire 22 in roundabout type, sample portion 4 are located at the upper end of resistance wire 22；One end of heat-conductive thermo tube and half
Conductor thermally conductive sheet 23 is connected, and the other end is connected with radiator.
In specific implementation, in the setting of the resistance wire 22 of roundabout type, the heating to sample portion 4 can be accelerated；And half
Under the mating reaction of conductor thermally conductive sheet 23 and resistance wire 22, it is to avoid heating process to environment temperature requires height, very flexible etc.
Shortcoming, error small, flexibility good the advantages of big with temperature-control range.
The lower end of the cabinet 1 of the fluorescent quantitation instrument is symmetrically arranged with sucker pin schematic diagram 10, the casing can be firmly pull-in on into thing
On body surface face, it is to avoid when upset fastens fluoroscopic examination portion 2, cause rocking for casing；Fluoroscopic examination portion 2 is provided with one groove 9,
It is easy to upset of the people to fluoroscopic examination portion 2；The bottom in fluoroscopic examination portion 2 is provided with a sealing ring 8, when fluoroscopic examination portion 2 and temperature
When the degree fastening of adjusting base 3 is integrated, it is ensured that its sealing.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or new using this practicality
Type.A variety of modifications to these embodiments will make it will be apparent that defined herein for those skilled in the art
General Principle can in other embodiments be realized in the case where not departing from the spirit or scope of utility model.Therefore, originally
Utility model will not be limited and the embodiments shown herein, and be to fit to and principles disclosed herein and novelty
The property consistent most wide scope of feature.
Claims (7)
1. a kind of fluorescent quantitation instrument, including cabinet, it is characterised in that：The cabinet include temperature adjustment base and with the temperature
The fluoroscopic examination portion that adjusting base one end is hinged；The temperature adjustment base is provided with a sample portion, the sample portion and set
Have in several jacks for being used to place PCR reaction tubes, the PCR reaction tubes and be filled with increased response agent, the sample portion
Material is aluminium alloy；The fluoroscopic examination portion is provided with a groove being engaged with the sample portion, the groove and is provided with
Several optical fiber being engaged with the jack visit mouth；
The fluoroscopic examination portion include being respectively used to launching blue light, the LED/light source portion of feux rouges and white light, exciter filter, two to
Look mirror, fiber coupling lens, y-type optical fiber, focus lamp, detection optical filter and photoelectric conversion module, the common port of the y-type optical fiber
Provided with self-focusing optical fiber, the self-focusing optical fiber is located at optical fiber and visited at mouth；The photoelectric conversion module is logical including what is be sequentially connected
Road formula photomultiplier and signal compensation circuit.
2. fluorescent quantitation instrument according to claim 1, it is characterised in that：The temperature adjustment base include from bottom to up according to
Radiator, semiconductor thermally conductive sheet, heat-conductive thermo tube and the resistance wire in roundabout type of secondary setting, the specimen locations are in the resistance
The upper end of silk；One end of the heat-conductive thermo tube is connected with the semiconductor thermally conductive sheet, and the other end is connected with the radiator.
3. fluorescent quantitation instrument according to claim 1, it is characterised in that：The increased response agent is glycerine, ox blood is pure
One or more in albumen, formamide, spermidine and glycine betaine.
4. fluorescent quantitation instrument according to claim 1, it is characterised in that：The lower end of the cabinet is symmetrically arranged with sucker
Pin.
5. fluorescent quantitation instrument according to claim 1, it is characterised in that：The fluoroscopic examination portion is provided with one groove.
6. fluorescent quantitation instrument according to claim 1, it is characterised in that：The bottom in the fluoroscopic examination portion is provided with a sealing
Circle.
7. fluorescent quantitation instrument according to claim 1, it is characterised in that：The focus lamp is prism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621345380.3U CN206378421U (en) | 2016-12-09 | 2016-12-09 | Fluorescent quantitation instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621345380.3U CN206378421U (en) | 2016-12-09 | 2016-12-09 | Fluorescent quantitation instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206378421U true CN206378421U (en) | 2017-08-04 |
Family
ID=59405930
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621345380.3U Active CN206378421U (en) | 2016-12-09 | 2016-12-09 | Fluorescent quantitation instrument |
Country Status (1)
| Country | Link |
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| CN (1) | CN206378421U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107817235A (en) * | 2017-12-11 | 2018-03-20 | 苏州合惠生物科技有限公司 | A kind of real time fluorescent quantitative augmentation detection instrument |
| CN110655043A (en) * | 2018-06-29 | 2020-01-07 | 圣奥化学科技有限公司 | Quenching device for producing insoluble sulfur |
| US11604146B2 (en) | 2017-09-19 | 2023-03-14 | Beckman Coulter, Inc. | Analog light measuring and photon counting with a luminometer system for assay reactions in chemiluminescence measurements |
-
2016
- 2016-12-09 CN CN201621345380.3U patent/CN206378421U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11604146B2 (en) | 2017-09-19 | 2023-03-14 | Beckman Coulter, Inc. | Analog light measuring and photon counting with a luminometer system for assay reactions in chemiluminescence measurements |
| US11754504B2 (en) | 2017-09-19 | 2023-09-12 | Beckman Coulter, Inc. | System for analog light measuring and photon counting in chemiluminescence measurements |
| CN107817235A (en) * | 2017-12-11 | 2018-03-20 | 苏州合惠生物科技有限公司 | A kind of real time fluorescent quantitative augmentation detection instrument |
| CN110655043A (en) * | 2018-06-29 | 2020-01-07 | 圣奥化学科技有限公司 | Quenching device for producing insoluble sulfur |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230203 Address after: No. 1618, Floor 16, Unit 1, Building 4, No. 1700, North Section of Tianfu Avenue, Chengdu Hi-tech Zone, China (Sichuan) Free Trade Pilot Zone, Chengdu, Sichuan, 610015 Patentee after: SICHUAN KEJIN BIOTECHNOLOGY CO.,LTD. Address before: No.6, Longjing Road, Longtan Temple, Chenghua District, Chengdu, Sichuan 610051 Patentee before: CHENGDU DANFENG TECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right |