CN206334397U - A kind of moving bed imitation chromatogram separation facility - Google Patents
A kind of moving bed imitation chromatogram separation facility Download PDFInfo
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- CN206334397U CN206334397U CN201621063670.9U CN201621063670U CN206334397U CN 206334397 U CN206334397 U CN 206334397U CN 201621063670 U CN201621063670 U CN 201621063670U CN 206334397 U CN206334397 U CN 206334397U
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Abstract
The purpose of this utility model is, there is provided a kind of moving bed imitation chromatogram separation facility, to belong to technical field of chromatography separation the problems such as more being fixed for existing SMB equipment operating modes.The device is made up of 8~30 root chromatogram columns, per root chromatogram column 6 intake lines, 4 output pipes and 1 circulation line are controlled by 11 two-port valves, all respective working conditions of two-port valve are controlled by PLC or single-chip computer control system, a variety of mode of operations can be set, including：The chromatographic column number for participating in separation is set, multi-zone is set, interception pipeline is set, setting area interband is continuous or independent operating process, sets either synchronously or asynchronously switch mode, the device pipeline is easy to connect, operation is flexible, easily safeguards, can complete multicomponent separation.
Description
Technical field
The utility model belongs to technical field of chromatography separation, more particularly to a kind of general-purpose simulation mobile bed chromatic separation dress
Put.
Background technology
SMBC (abbreviation SMB) technology have high separation, high yield, in high yield, high efficiency and company
Continuous automatic running scheme, is high-end separation means.Simulated movable bed chromatography device is connected into circulation loop by some tail prime ministers
Chromatographic column is constituted, by regularly moving mobile phase import with the position of efflux outlet along the flow direction of mobile phase come mould
The move counter-current for intending stationary phase and mobile phase flows, and then realizes the separation of different component.SMB mode of operations, which include setting, to be participated in
The chromatographic column number of separation, division difference in functionality area, each area's chromatographic column of setting, SMBC separation principle determine work
The diversity of operation mode；And mode of operation is that Optimized Simulated mobile bed chromatic separates the key factor examined or check first, but actual
On SMB equipment mostly restricted by switching valve and its software kit using more fixed operational mode, same SMB equipment
It is difficult to realize the conversion between a variety of different modes.
Utility model content
The utility model provides a kind of general-purpose simulation mobile bed chromatic separator.The device is each in SMB systems
Chromatographic column arranges 11 two-port valves, can set a variety of mode of operations, realize multicomponent separation.
A kind of Simulation moving bed device, including M root chromatogram columns, 4≤M≤30 connect respectively per root chromatogram column entrance and exit
Lead to access port more and export and lead to more, many reduction of fractions to a common denominators of entrance do not connect the circulation line of 6 intake lines and previous root chromatogram column, go out
The many reduction of fractions to a common denominators of mouth do not connect 4 output pipes and the circulation line of current chromatographic column；Every described intake line or efferent duct
Road is connected with corresponding input bus or output bus respectively；Material liquid, eluent input phase by corresponding input bus respectively
Answer the intake line of chromatographic column；It is many that described circulation line connects the previous many logical and current chromatogram column inlets in root chromatogram column outlet
It is logical, all chromatographic columns is connected by circulation line tail head；Except circulate efflux in addition to various column effluents respectively by
Corresponding chromatographic column is output to corresponding output bus by corresponding output pipe；
Material liquid, eluent are stored in corresponding fluid reservoir；From output bus flow out various column effluents be
The system efflux of Simulation moving bed is stored in corresponding fluid reservoir；
Two-port valve is respectively provided with intake line, output pipe and circulation line per root chromatogram column；The two-port valve is by electricity
Magnet valve and check (non-return) valve composition, and control each two-port valve to be turned on and off state by PLC or single-chip computer control system；
The chromatographic column works under the constant temperature in the range of -10 DEG C~200 DEG C；
Above-mentioned Simulation moving bed device, can set multi-zone SMB：Such as three band a-b-c, four band SMB：A-b-c-d, five
Band SMB：The bands of a-b-c-d-e six are to more bands, and a, b, c, d, e refer to every band chromatographic column number, and open loop can be set to input material liquid
Or the pattern of eluent pattern, adsorption zone and the fine Disengagement zone of slow component are continuous, the pattern of elution zone independence, each area's independence work
The order SMB operational modes of work.
Further, above-mentioned Simulation moving bed device, may be selected the chromatographic column number that SMB participates in separation, i.e.,：Described
The chromatographic column that separation process is participated in device is N roots, 3≤N≤M；N root chromatogram columns are connected by circulation line tail head, are formed back
Road.
Further, above-mentioned Simulation moving bed device, an output bus is connected with an input bus, is constituted and is cut
Take pipeline.
Further, above-mentioned Simulation moving bed device, it is possible to achieve simultaneously continuous backflow is refined interception part efflux, is beaten
Drive the output pipe two-port valve being connected with the interception pipeline and latter color of the post of the chromatographic column of current output efflux
The intake line two-port valve being connected with the interception pipeline of spectrum post, constitutes return line, and set defeated on return line
Send pump；
Or 1 threeway and 1 delivery pump are set in interception pipeline, a mouth of the threeway, which is used to export, to flow out
Liquid, while opening the output pipe two-port valve that is connected with the interception pipeline and the post of the chromatographic column of current output efflux
The intake line two-port valve being connected with the interception pipeline of latter root chromatogram column, equally constitutes return line；
, will be current also, above two is constituted in the mode of return line, when the return line of current chromatographic column is opened
Chromatographic column circulation line is closed.
Or, above-mentioned Simulation moving bed device, it is possible to achieve interception part efflux simultaneously feeds back to sample introduction entrance or washed
De- liquid entrance, opens the output pipe two-port valve being connected with the interception pipeline of the chromatographic column of current output efflux and except this
Outside the latter root chromatogram column of post any one root chromatogram column with the intake line two-port valve that is connected of interception pipeline, composition around
Cross pipeline；
It is described to bypass 0~1 delivery pump of setting in pipeline.
Further, above-mentioned Simulation moving bed device, can set either synchronously or asynchronously switch mode, for same work
Operation mode, in switching cycle, sets import, outlet port by control system time segment, adjusts each functional areas band
Length is each band chromatographic column number.
Further, above-mentioned Simulation moving bed device, the serial number that described M root chromatogram columns are arranged in order：Z1、Z2、
Z3, Z4, Z5 ..., Z (n-1), Zn, Z (n+1) ..., ZM, then in described N root chromatogram columns, come the adjacent chromatographic column at two ends
Tail head is connected, and other chromatographic column tail head separately are connected, and it is the circulation line, all circulation pipes that chromatographic column tail head, which is connected,
Road is isometric；
When N is even number, the connected mode of N root chromatogram columns is：Z1、Z3、Z5、…、Z(N-3)、Z(N-1)、ZN、Z(N-2)、Z
(N-4) ..., Z4, Z2, Z1, i.e.,：The direction increased by odd number, odd number chromatographic column tail prime minister is connected to one end Z (N-1), Z (N-1) with
ZN tails head is connected, then, and direction is reduced by even number, and even number chromatographic column tail prime minister is connected to the other end Z2, Z2 and Z1 tail head and is connected, structure
It is that " fried dough twist formula " tail head is connected into the loop of N root chromatogram columns；
When N is odd number, the connected mode of N root chromatogram columns is：Z1、Z3、Z5、…、Z(N-4)、Z(N-2)、ZN、Z(N-1)、Z
(N-3) ..., Z4, Z2, Z1, i.e.,：The direction increased by odd number, odd number chromatographic column tail prime minister is connected to one end ZN, ZN and Z (N-1) tail
Head is connected, then, and direction is reduced by even number, and even number chromatographic column tail prime minister is connected to the other end Z2, Z2 and Z1 tail head and is connected, and constitutes N
The loop of root chromatogram column, is that " fried dough twist formula " tail head is connected；
The connecting pipeline by the way of " fried dough twist formula " tail head is connected, can make the liquid circulated of circulation line has approximately
Course of conveying, it is to avoid differential diffusion.
Further, above-mentioned Simulation moving bed device, the arrangement mode of the M root chromatogram columns is word order, annular
During the mode such as arrangement or rectangular arranged, " fried dough twist formula " tail head is connected convenient, and pipeloop is isometric.
Further, above-mentioned Simulation moving bed device, settable multiple-working mode works simultaneously, by N root chromatogram columns
Simulation moving bed be decomposed into 2 sets of Simulation moving bed systems, i.e.,：L root chromatogram column tails head is connected to form a set of Simulation moving bed, 3
≤ L≤(N-3), residue (N-L) root chromatogram column tail head is connected to form other set Simulation moving bed, 2 sets of Simulation moving bed systems
For independent operation mode, parallel running mode or series operation pattern.
Further, above-mentioned Simulation moving bed device, disconnects the connecting pipeline between 2 sets of Simulation moving beds, being capable of structure
Into the different Simulation moving bed systems of number set, number set Simulation moving bed system is independent operation mode, parallel running mode or string
Through transport row mode.
Further, all chromatographic columns are arranged at a region by above-mentioned Simulation moving bed device, and all two-port valves are set
Another region is placed in, temperature control is carried out to chromatogram columnar region.
Further, filter, on-line degassing machine, circulation pipe are equipped with above-mentioned Simulation moving bed device, input bus
The detector that flowmeter, solution concentration and purity are equipped with flowmeter, output bus is equipped with road.
Further, above-mentioned Simulation moving bed device, with pipeline that mode of operation is unrelated can corresponding how logical or
Sealed at threeway with plug, to extend the related two-port valve life-span.
Compared with prior art, advantage of the present utility model is：
1st, the present apparatus has a variety of functions：SMB is set to participate in the chromatographic column number of separation；Multi-zone SMB is set：Such as three bands
A-b-c, four band SMB：A-b-c-d, five band SMB：A-b-c-d-e, six bands are to more bands, and a, b, c, d refer to every band chromatographic column number；
Setting area interband is continuously run or independent operation mode；Open loop sample introduction and the pattern of sampling are set；Setting either synchronously or asynchronously switches
Pattern；Interception part efflux simultaneously feeds back to sample introduction entrance or eluent entrance；Multiple-working mode works simultaneously；Complete multigroup
Separation etc..
2nd, two-port valve operation is flexible, easily safeguards.
3rd, pipeline is easy to connect.
4th, valve and chromatographic column subregion are placed, can be to the overall temperature control of chromatographic column.
Brief description of the drawings
Fig. 1 is a kind of Simulation moving bed schematic device of 8 root chromatogram column, and the Simulation moving bed is a loop, eight
Zone, 1-1-1-1-1-1-1-1, the strong absorbed component A of three components of separation, intermediate adsorption component B and weakly adsorbed components C.
Fig. 2 is a kind of Simulation moving bed schematic device of 8 root chromatogram column, and the Simulation moving bed is a loop, six
Zone, 1-1-2-1-1-2 separates three component A, B and C.Can be at corresponding many logical or threeways with the pipeline that mode of operation is unrelated
Sealed with plug.
Fig. 3 is a kind of Simulation moving bed schematic device of 8 root chromatogram column, and the Simulation moving bed is a loop, four
Zone, 2-2-2-2 separates two components A, B.
Fig. 4 is a kind of Simulation moving bed schematic device of 8 root chromatogram column, and the Simulation moving bed is a loop, six
Zone, 1-1-2-1-1-2, wherein first band independence and gradient elution separate three component A, B and C.
Fig. 5 is a kind of Simulation moving bed schematic device of 8 root chromatogram column, execution sequence chromatographic isolation, 4 root chromatogram columns ginseng
With separating, each band independent operating, or separation four sections of fraction As, B, C and W, or use gradient elution.
Fig. 6 is a kind of Simulation moving bed schematic device of 8 root chromatogram column, and the Simulation moving bed is " fried dough twist formula " tail prime minister
Even.
Fig. 7 is a kind of Simulation moving bed schematic device of 8 root chromatogram column, and the Simulation moving bed is " fried dough twist formula " tail prime minister
Even, two 1-1-2 Simulation moving beds loops, separation component A, B and C.
Wherein, Zn：N-th root chromatogram column, n：Chromatographic column sequence number, Lmi：Input or output bus, m=1 represent input, m=2
Output is represented, i is input or output bus sequence number, Lnmi：N-th root chromatogram column input/output pipeline；Vnmi：Lnmi two-way
Valve, Tn：Circulation line two-port valve；
F：Material liquid fluid reservoir, D or Dq：Eluent D or eluent Dq fluid reservoirs, q：Eluent sequence number, A：Separation component A
Fluid reservoir, B：Component B fluid reservoirs, C：Component C fluid reservoirs, W：Component W fluid reservoirs, Pi：The delivery pump of i-th input bus.
Embodiment
The utility model is described in further detail as embodiment using 8 root chromatogram column Simulation moving bed devices below.
Embodiment 1
A kind of Simulation moving bed device, including 8 identical chromatographic columns, the n-th root chromatogram column Zn entrance and exits connect respectively
Lead to access port more and export and lead to more, many reduction of fractions to a common denominators of entrance do not connect the circulation line T (n- of 6 intake lines and previous root chromatogram column
1) many reduction of fractions to a common denominators, are exported and do not connect 4 output pipes and the circulation line Tn of current chromatographic column, totally 11 pipelines；Material liquid, elution
Liquid is pumped into respective intake line by corresponding delivery pump via input bus respectively；Circulation line connects previous root chromatogram column Z
(n-1) outlet and current chromatographic column Zn entrances, make all chromatographic column tail head be connected；Various chromatographic columns in addition to efflux is circulated
Efflux imports corresponding output bus via respective output pipe respectively；Material liquid, eluent are stored in corresponding liquid storage
In tank；The solution flowed out from output bus is that the system efflux of Simulation moving bed is stored in corresponding fluid reservoir；Chromatographic column
All intake lines, all output pipes and all circulation lines be respectively provided with two-port valve；Chromatographic column works at room temperature；Two-way
Valve is made up of magnetic valve and check (non-return) valve, controls all respective working conditions of two-port valve by PLC or single-chip computer control system, or open
Or close.Carrying out practically step is embodied by the open and close state of each two-port valve, here, only describing the opening state of chromatographic column, is closed
Closed state is not repeated, and the Tn that chromatographic column circulation line two-port valve is only set with Way out is represented.
Simulation moving bed device shown in Fig. 1 is specially：Provided with a loop, eight zone, 1-1-1-1-1-1-1-1,
Three components are separated, respectively strong absorbed component A, intermediate adsorption component B and weakly adsorbed components C；
In the apparatus, material liquid is pumped into input bus L11 in fluid reservoir F by infusion pump P1, and eluent is in fluid reservoir D
In component A be pumped into after input bus L12, separation through output bus L24 by infusion pump P2 enter fluid reservoir A, component B is total through output
Line L23 enters fluid reservoir B, and component C enters fluid reservoir C through output bus L22；
An output bus therein and an input bus therein are connected, interception pipeline, L24-L16, L23- is constituted
L15、L22-L14、L21-L13；
One threeway and platform mobile phase delivery pump P6 are set in interception pipeline L24-L16, and the mouth output of threeway should
The system efflux A of pipeline is intercepted, the chromatographic column Z1 for opening current output efflux A is connected with interception pipeline L24-L16
The output pipe two-port valve V124 and latter root chromatogram column Z2 intake line two-port valve being connected with interception pipeline L24-L16
V216, opens interception pipeline, simultaneously closes off chromatographic column Z1 efflux A circulation line T1, formed chromatographic column Z1 and chromatographic column Z2 it
Between efflux A return lines；
Similarly set in interception pipeline L23-L15 and L22-L14, the efflux formed between chromatographic column Z3 and chromatographic column Z4
Efflux C return lines between B return lines, and chromatographic column Z7 and chromatographic column Z8；
1 mobile phase delivery pump P3 is set in interception pipeline L21-L13, current output A, B mixture efflux is opened
Chromatographic column Z5 with interception pipeline L21-L13 the output pipe two-port valve V521 that is connected and chromatographic column Z3 and interception pipeline
The intake line two-port valve V313 that L21-L13 is connected, the efflux AB's formed between chromatographic column Z5 and chromatographic column Z3 bypasses
Pipeline.
The mode of operation of the device is specially：
Z1：V112 and V124 are opened, and with eluent D elution fraction A, stationary phase regeneration, a component A effluxes part flows into storage
Flow container A a, part is back to Z2 by return line L24-L16；
Z2：V216 and T2 are opened, and component A is finely separated with B；
Z3：V313 and V323 are opened, and component A, B is adsorbed and separated, and component A, B mixed liquor is carried by bypassing pipeline L21-L13
For a component B effluxes part flows into fluid reservoir B, and a part is back to Z4 by return line L23-L15；
Z4：V415 and T4 are opened, and reclaim component B, mobile phase regeneration；
Z5：V521 and T5 are opened, elution fraction A, B mixture, stationary phase regeneration, A, B mixture efflux a part through around
Cross pipeline L21-L13 and flow into Z3, a part is back to Z6 by return line L23-L15；
Z6：T6 is opened, and component A, B mixture and component C are finely separated；
Z7：V711 and V722 are opened, and A, B, C of material liquid are adsorbed and separated, and a component C effluxes part flows into fluid reservoir
C a, part is back to Z8 by return line L22-L14；
Z8：V814 and T8 are opened, and reclaim component C, mobile phase regeneration.
When reaching switching time, mode of operation is moved to next root chromatogram column in loop by control system along mobile phase direction,
The process is repeated, and the move counter-current of simulation stationary phase and mobile phase flows, and by adsorption and desorption and rectifying, realizes 3 groups
Separation.
Simulation moving bed device shown in Fig. 2 is specially：Provided with a loop, six zone, 1-1-2-1-1-2, separation
Three component A, B and C.
Sealed in the outlet for being passed through mouth and threeway more using plug, close the pipeline Ln14 and L22- unrelated with mode of operation
14, while the former L23-L15 outlets for intercepting pipeline threeway are sealed with plug, D2 eluents are conveyed with P5.
The mode of operation of the device is specially：
Z1：V112 and V124 are opened, and with eluent D1 elution fraction A, stationary phase regeneration, a component A effluxes part is flowed into
Fluid reservoir A a, part is back to Z2 by return line L24-L16；
Z2：V216 and T2 are opened, and component A is finely separated with B；
Z3 and Z4：V313, T3 and V423 are opened, and component A, B is adsorbed and separated, and component A, B mixed liquor is by bypassing pipeline
L21-L13 is provided, and component B effluxes flow into fluid reservoir B；
Z5：V515, V521 and T5 are opened, with D2 elution fraction A and B, stationary phase regeneration, an A and B mixtures efflux part
Z3 is flowed into through bypassing pipeline, a part is recycled to Z6；
Z6：T6 is opened, and component A, B mixture and component C are finely separated；
Z7 and Z8：V711, T7 and V822 are opened, and A, B, C of material liquid are adsorbed and separated, and component C effluxes flow into liquid storage
Tank C.
When reaching switching time, mode of operation is moved to next root chromatogram column in loop by control system along mobile phase direction,
The process is repeated, and the move counter-current of simulation stationary phase and mobile phase flows, and by adsorption and desorption and rectifying, realizes 3 groups
Separation.
Simulation moving bed device shown in Fig. 3 is specially：Provided with a loop, tetra- zone of 2-2-2-2 separate two components
A、B。
The mode of operation of the device is specially：
Z1 and Z2：V112, T1, V224 are opened, with eluent D elution fraction A, stationary phase regeneration, a component A effluxes part
Fluid reservoir A is flowed into, a part is back to Z3 by return line L24-L16；
Z3 and Z4：V316, T3, T4 are opened, and component A is finely separated with B；
Z5 and Z6：V511, T5 and V623 are opened, and component A, B is adsorbed and separated, and a component B effluxes part flows into liquid storage
Tank B a, part is back to Z7 by return line L23-L15；
Z7 and Z8：V715, T7 and T8 are opened, and reclaim component B, mobile phase regeneration；
When reaching switching time, mode of operation is moved to next root chromatogram column in loop by control system along mobile phase direction,
The process is repeated, and the move counter-current of simulation stationary phase and mobile phase flows, and by adsorption and desorption and rectifying, realizes 2 groups
Separation.
For tetra- zone of 2-2-2-2, divide t1, t2, t3, t4 period successively switching cycle Ts,
t1：Component B is exported, next root chromatogram column is moved to along mobile phase direction, switching cycle is Ts；
t2：By eluent entrance, next root chromatogram column is moved to along mobile phase direction, switching cycle is Ts；
t3：Component A is exported, next root chromatogram column is moved to along mobile phase direction, switching cycle is Ts；
t4：By charging aperture, next root chromatogram column is moved to along mobile phase direction, switching cycle is Ts；
Ts：Restore, tetra- zone of 2-2-2-2.
Said process repeats, and it is zone length that thus asynchronised handover, which changes each band chromatographic column number,.
Simulation moving bed device shown in Fig. 4 is specially：One loop of setting, six zone, 1-1-2-1-1-2, wherein
First band independence and gradient elution, separate three component A, B and C.
Eluent D1 is pumped into input bus L12 in fluid reservoir D1 by infusion pump P2, eluent D2 in fluid reservoir D2 by
Infusion pump P4 is pumped into input bus L14, and eluent D3 is pumped into input bus L15, eluent in fluid reservoir D3 by infusion pump P5
D4 is pumped into input bus L16 in fluid reservoir D4 by infusion pump 6；The threeway original interception pipeline of L24, L23, L22 output bus goes out
Sealed at mouthful with plug.
Mode of operation is specially：
Z1：V112, V114 and V124 are opened, with eluent D1 and D2 gradient elution component A, stationary phase regeneration, component A outflows
Liquid flows into fluid reservoir A；
Z2：V215 and T2 are opened, and mobile phase, fine separation component A and B are made with D3；
Z3 and Z4：V313, T3 and V423 are opened, and component A, B is adsorbed and separated, and component A, B mixed liquor is by bypassing pipeline
L21-L13 is provided, and component B effluxes flow into fluid reservoir B；
Z5：V516, V521 and T5 are opened, with D4 elution fraction A and B, stationary phase regeneration, an A and B mixtures efflux part
Z3 is flowed into through bypassing pipeline, a part is recycled to Z6；
Z6：T6 is opened, and component A, B mixture and component C are finely separated；
Z7 and Z8：V711, T7 and V822 are opened, and mixture A, B, C are adsorbed and separated, and component C effluxes flow into fluid reservoir
C。
Simulation moving bed device shown in Fig. 5 is specially：Setting Z1~Z4, totally 4 root chromatogram columns participate in separation, execution sequence
Chromatographic isolation, each band independent operating can carry out separating four sections of fraction As, B, C and W, or carry out gradient elution.
Eluent D1 is pumped into input bus L12 in fluid reservoir D1 by infusion pump P2, eluent D2 in fluid reservoir D2 by
Infusion pump P3 is pumped into input bus L13, and eluent D3 is pumped into input bus L14 in fluid reservoir D3 by infusion pump P4；Respectively by
L24, L23, L22 and L21 export component A to fluid reservoir A, component B to fluid reservoir B, component C to fluid reservoir C and component W to liquid storage
Tank W；Sealed at the threeway original interception tube outlet of L24, L23, L22 output bus with plug
Mode of operation is specially：
Z1：V114, V124 are opened, and with eluent D1 elution fraction A, stationary phase regeneration, component A effluxes flow into fluid reservoir A；
Z2：V213 and V223 are opened, and mobile phase, fine separation component B are made with D2；
Z3：V312 and V322 are opened, and mobile phase, fine separation component C are made with D3；
Z4：V411 and V421 are opened, and component A, B, C, the W for entering sample liquid are adsorbed and separated, and W effluxes flow into fluid reservoir B；
Simulation moving bed device shown in Fig. 6 is specially that " fried dough twist formula " tail head of chromatographic column is connected.
Here only consider the set-up mode of chromatogram intercolumniation circulation line, working condition is not considered.8 root chromatogram columns are arranged in order
Serial number：Z1, Z2, Z3, Z4, Z5, Z6, Z7, Z8, setting N root chromatogram columns participate in separation process, chromatogram during 3≤N≤8, N=8
The connected mode of post is：Z1, Z3, Z5, Z7, Z8, Z6, Z4, Z2, Z1, i.e.,：Two ends Z7 is connected with Z8 tails head, Z2 and Z1 tail prime ministers
Even, other interval chromatographic column tail head are connected, and constitute the loop of 8 root chromatogram columns；When selection participates in the chromatographic column number of separation, this
Plant circulation line isometric, and connected mode is simple to operate, such as：Be converted to as N=6 or by the connected mode of chromatographic column during N=8
During N=6, Z5 circulation lines are connected in Z6 arrival ends, link circuit is：Z1, Z3, Z5, Z6, Z4, Z2, Z1, i.e.,：Two ends Z5
It is connected with Z6 tails head, Z2 is connected with Z1 tails head, other interval chromatographic column tail head are connected, and constitute the loop of 6 root chromatogram columns；Work as N=7
When or when the connected mode of chromatographic column during N=8 is converted into N=7, Z6 circulation lines are connected in Z7 arrival ends, connected back to
Lu Wei：Z1, Z3, Z5, Z7, Z6, Z4, Z2, Z1, i.e.,：Two ends Z7 is connected with Z6 tails head, Z2 is connected with Z1 tails head, other interval colors
Compose post tail head to be connected, constitute the loop of 7 root chromatogram columns；
By the setting of this circulation line, the device of the present embodiment readily can be constituted into many set moving beds, for example,
During N=8, Z3 circulation lines are connected in Z4 arrival ends, Z6 circulation lines are connected in Z5 arrival ends, you can two sets of each 4 posts are constituted
Moving bed, two sets of moving bed systems can be arranged to independent operating (can be different mobile phases, it is different stationary phase, different former
Feed liquid), parallel running, or series operation.
Simulation moving bed device shown in Fig. 7 is specially：" fried dough twist formula " tail head of chromatographic column is connected, two 1-1-2 simulations
Moving bed loop, separation component A, B and C.
Eluent D1 is pumped into input bus L12 in fluid reservoir D1 by infusion pump P2, eluent D2 in fluid reservoir D2 by
Infusion pump P5 is pumped into input bus L15.Sealed at the threeway original interception tube outlet of L23, L22 output bus with plug.
Mode of operation is specially：
The first loop order of connection is：Z1, Z3, Z4, Z2 and Z1；
Z1：V112 and V124 are opened, and with eluent D1 elution fraction A, stationary phase regeneration, a component A effluxes part is flowed into
Fluid reservoir A a, part is back to Z3 through return line L24-L16；
Z3：V316 and T3 are opened, and component A is finely separated with B；
Z4 and Z2：V413, T4 and V223 are opened, and component A, B is adsorbed and separated, and component A, B mixed liquor is by bypassing pipeline
L21-L13 is provided, and component B effluxes flow into fluid reservoir B；
The second loop order of connection is：Z5, Z7, Z8, Z6 and Z5.
Z5：V515, V521 and T5 are opened, with D2 elution fraction A and B, stationary phase regeneration, an A and B mixtures efflux part
Z4 is flowed into through bypassing pipeline, a part is recycled to Z7；
Z7：T7 is opened, and component A, B mixture and component C are finely separated；
Z8 and Z6：V811, T8 and V622 are opened, and mixture A, B, C are adsorbed and separated in material liquid, component C efflux streams
Enter fluid reservoir C.
The connecting line between 2 sets of Simulation moving beds is disconnected, 3~7 sets of Simulation moving bed control systems are may be constructed, is equipped with
Pump, pipeline, threeway and fluid reservoir, carry out 4-14 component separation.
Claims (9)
1. a kind of moving bed imitation chromatogram separation facility, including M root chromatogram columns, it is characterised in that 4≤M≤30, per root chromatogram column
Entrance and exit to connect lead to entrance more and export respectively to be led to more, and many reduction of fractions to a common denominators of entrance do not connect 6 intake lines and previous color
The circulation line of post is composed, many reduction of fractions to a common denominators is exported and does not connect 4 output pipes and the circulation line of current chromatographic column；Described every
Intake line or output pipe are connected with corresponding input bus or output bus respectively；Material liquid, eluent are respectively by corresponding
Input bus input the intake line of corresponding chromatographic column；Described circulation line connects previous root chromatogram column outlet and leads to and work as more
Preceding chromatogram column inlet leads to more, all chromatographic columns is connected by circulation line tail head；Various chromatograms in addition to efflux is circulated
Post efflux is output to corresponding output bus by corresponding chromatographic column by corresponding output pipe respectively；
Material liquid, eluent are stored in corresponding fluid reservoir；The various column effluents flowed out from output bus are simulation
The system efflux of moving bed is stored in corresponding fluid reservoir；
Two-port valve is respectively provided with intake line, output pipe and circulation line per root chromatogram column；The two-port valve is by magnetic valve
Constituted with check (non-return) valve, and control each two-port valve to be turned on and off state by PLC or single-chip computer control system；
The chromatographic column works under the constant temperature in the range of -10 DEG C~200 DEG C；
Wherein, the chromatographic column that separation process is participated in said device is N roots, and 3≤N≤M, N root chromatogram columns pass through circulation line
Tail head is connected, and forms loop.
2. a kind of moving bed imitation chromatogram separation facility according to claim 1, it is characterised in that by an output bus
It is connected with an input bus, constitutes interception pipeline.
3. a kind of moving bed imitation chromatogram separation facility according to claim 2, it is characterised in that open current output stream
Go out liquid chromatographic column with the latter root chromatogram column of the output pipe two-port valve that is connected of interception pipeline and the post with it is described
The connected intake line two-port valve of pipeline is intercepted, return line is constituted, and delivery pump is set on return line；
Or 1 threeway and 1 delivery pump are set in interception pipeline, a mouth of the threeway is used to export efflux, together
When open current output efflux chromatographic column the output pipe two-port valve being connected with the interception pipeline and the post it is latter
The intake line two-port valve being connected with the interception pipeline of root chromatogram column, equally constitutes return line；
Also, above two is constituted in the mode of return line, when the return line of current chromatographic column is opened, by current chromatogram
Post circulation line is closed.
4. a kind of moving bed imitation chromatogram separation facility according to claim 2, it is characterised in that open current output stream
The local official for going out the output pipe two-port valve being connected with the interception pipeline of the chromatographic column of liquid and the latter root chromatogram column except the post
Anticipate a root chromatogram column with the intake line two-port valve that is connected of interception pipeline, constitute and bypass pipeline；
It is described to bypass 0~1 delivery pump of setting in pipeline.
5. a kind of moving bed imitation chromatogram separation facility according to claim 1, it is characterised in that for same work
Pattern, in switching cycle, sets import, outlet port by control system time segment, adjusts the length of each functional areas band
Degree is each band chromatographic column number.
6. a kind of moving bed imitation chromatogram separation facility according to any one in Claims 1 to 5, it is characterised in that
The serial number that described M root chromatogram columns are arranged in order：Z1, Z2, Z3, Z4, Z5 ..., Z (n-1), Zn, Z (n+1) ..., ZM, then exist
In described N root chromatogram columns, the adjacent chromatographic column tail head for coming two ends is connected, and other chromatographic column tail head separately are connected, color
It is the circulation line to compose post tail head to be connected, and all circulation lines are isometric；
When N is even number, the connected mode of N root chromatogram columns is：Z1、Z3、Z5、…、Z(N-3)、Z(N-1)、ZN、Z(N-2)、Z(N-
4) ..., Z4, Z2, Z1, i.e.,：The direction increased by odd number, odd number chromatographic column tail prime minister is connected to one end Z (N-1), Z (N-1) and ZN
Tail head is connected, then, and direction is reduced by even number, and even number chromatographic column tail prime minister is connected to the other end Z2, Z2 and Z1 tail head and is connected, constitutes
The loop of N root chromatogram columns, is that " fried dough twist formula " tail head is connected；
When N is odd number, ZN is connected with Z (N-1) tail head, other identical for the situation of even number with N.
7. a kind of moving bed imitation chromatogram separation facility according to claim 1, it is characterised in that by N root chromatogram columns
Simulation moving bed be decomposed into 2 sets of Simulation moving bed systems, i.e.,：L root chromatogram column tails head is connected to form a set of Simulation moving bed, 3
≤ L≤(N-3), residue (N-L) root chromatogram column tail head is connected to form other set Simulation moving bed, 2 sets of Simulation moving bed systems
For independent operation mode, parallel running mode or series operation pattern.
8. a kind of moving bed imitation chromatogram separation facility according to claim 1, it is characterised in that set all chromatographic columns
A region is placed in, all two-port valves are arranged at another region, temperature control is carried out to chromatogram columnar region.
9. a kind of moving bed imitation chromatogram separation facility according to claim 1, it is characterised in that be equipped with input bus
It is equipped with filter, on-line degassing machine, circulation line on flowmeter, output bus and is equipped with flowmeter, solution concentration and purity
Detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621063670.9U CN206334397U (en) | 2016-09-19 | 2016-09-19 | A kind of moving bed imitation chromatogram separation facility |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201621063670.9U CN206334397U (en) | 2016-09-19 | 2016-09-19 | A kind of moving bed imitation chromatogram separation facility |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106166402A (en) * | 2016-09-19 | 2016-11-30 | 辽宁科技大学 | A kind of moving bed imitation chromatogram separation facility |
| CN110025983A (en) * | 2019-05-17 | 2019-07-19 | 山东兆光色谱分离技术有限公司 | A kind of chromatographic fractionation system and its separation method |
| CN115485046A (en) * | 2020-05-14 | 2022-12-16 | 奥加诺株式会社 | Simulated moving bed mode chromatographic separation method and simulated moving bed mode chromatographic separation system |
-
2016
- 2016-09-19 CN CN201621063670.9U patent/CN206334397U/en not_active Withdrawn - After Issue
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106166402A (en) * | 2016-09-19 | 2016-11-30 | 辽宁科技大学 | A kind of moving bed imitation chromatogram separation facility |
| CN106166402B (en) * | 2016-09-19 | 2018-06-29 | 辽宁科技大学 | A kind of moving bed imitation chromatogram separation facility |
| CN110025983A (en) * | 2019-05-17 | 2019-07-19 | 山东兆光色谱分离技术有限公司 | A kind of chromatographic fractionation system and its separation method |
| CN115485046A (en) * | 2020-05-14 | 2022-12-16 | 奥加诺株式会社 | Simulated moving bed mode chromatographic separation method and simulated moving bed mode chromatographic separation system |
| CN115485046B (en) * | 2020-05-14 | 2024-03-12 | 奥加诺株式会社 | Simulated moving bed type chromatographic separation method and simulated moving bed type chromatographic separation system |
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