CN206253144U - Closed test chamber - Google Patents
Closed test chamber Download PDFInfo
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- CN206253144U CN206253144U CN201621333068.2U CN201621333068U CN206253144U CN 206253144 U CN206253144 U CN 206253144U CN 201621333068 U CN201621333068 U CN 201621333068U CN 206253144 U CN206253144 U CN 206253144U
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- temporary room
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Abstract
The utility model is related to a kind of closed test chamber, it includes container portion and cap, container portion is used to receive sample to be detected, cap is used for the opening in closed container portion, at least one inspection medicament temporary room is formed with inwall at the opening in container portion, when cap closed container portion, cap is simultaneously closed to check medicament temporary room so that inspection medicament temporary room is not connected with the part around inspection medicament temporary room in container portion；The convex top for being capable of elastic deformation is formed with position in cap for closed inspection medicament temporary room, with container portion opposite side protrusion of the convex top towards cap, and the thorn post extended towards container portion is provided with convex top, when convex top is depressed, piercing the lower end of post can pierce through the bottom of inspection medicament temporary room.The closed test chamber in whole process of the test can guarantee test container inside it is completely closed, so as to bacterial invasion will not be produced when medicament is added in test sample in container.
Description
Technical field
The utility model is related to a kind of closed test chamber, more particularly to adds medicine in test sample in container
The closed test chamber of bacterial invasion will not be produced during agent.
Background technology
Detection of Cytokines is an important component of cellular immune function detection.The current detecting cell factor
Level mainly includes two class methods：One is the detection of BA, and this kind of method is using a certain specific life of cell factor
The designed detection method of thing effect, such as IL-2 maintains the propagation of activating T cell, and IFN can protect virus to normal cell
Infection, TNF-α selectively kills some tumour cells etc., therefore sensitiveness is also higher；Two is quantitative detection, commonly uses enzyme linked immunological
Adsorption test (ELISA), such as polyclonal antibody and monoclonal antibody, recognize the double antibody folder of two kinds of monoclonal antibodies of different epitopes
Heart method, biology and quantitative detection method respectively have advantage and disadvantage, can be detected simultaneously when necessary.
Which kind of, however, no matter being detected using method, it is required for adding a variety of inspection medicaments in the sample.For example,
When the IL-1 acceptors in mouse chest cell carry out BA detection, the mouse that will be obtained under gnotobasis is first had to
Thymus gland is placed in a reservoir, grinds to form cell suspension.Then pure water is injected with syringe, cell suspension is adjusted to predetermined concentration
Dilution, so as to obtain the sample of detection.Then to IL-1 standard items are added in sample, 10~20 are cultivated at 37 DEG C small
When.The confection of 3H-TdR, POPOP, PPP and dimethylbenzene is subsequently adding, 5 hours are stood.Then, with 9999 type glass fibres
Filter paper is filtered to sample solution, and final detection is collected to the cell on filter paper and carried out using cell collector.
It is readily appreciated that, in order to ensure that sample will not be contaminated, in the overall process for being prepared into final detection from sample
In, it is necessary to be carried out in gnotobasis.In other words, in whole detection process, any bacterium or virus can not be mixed into sample.Cause
This, typically can carry out this detection in the environment of such as desinfection chamber etc..
However, because the process of this detection is very long, one-time detection often spends the time of several days.Generate for a long time
Occupy the problem of desinfection chamber.In order to avoid long-time occupies desinfection chamber, can sometimes use and sample is obtained in desinfection chamber, then
Sample is sealed up for safekeeping in closed test chamber (for example, closed box) and is placed in common environment.According to the requirement of experiment,
Various inspection medicaments are injected in the predetermined moment passes through syringe to closed test chamber.Reaching the culture that satisfaction is required
Closed container is opened after time, sample is taken out and is filtered and detected.
When various inspection medicaments are injected in test chamber, as syringe is pierced into closed container from outside, on the one hand
There is bacterium to be attached on syringe to enter into closed container, another aspect syringe leaves on closed container
Cut be also possible to form the path during bacterium invades closed container.Therefore, it is difficult to avoid the intrusion of bacterium completely.Especially
It is that the time is more long during long-time culture sample, bacterium is easier to invade closed container by cut.
Therefore, it is desired to occur it is a kind of not only without long-time occupied desinfection chamber again will not added in sample check medicament when
Produce the technological means of bacterial invasion.
Utility model content
The utility model is a kind of closed test chamber, and it can guarantee test container in whole process of the test
It is internal completely closed, so as to bacterial invasion will not be produced when adding medicament in the test sample in container.
To achieve the above object, the utility model provides following technical scheme.
A kind of closed test chamber, it includes container portion and cap, and the container portion is used to receive sample to be detected,
The cap is used for the opening in the closed container portion, wherein, it is formed with least one on the inwall at the opening in the container portion
Individual inspection medicament temporary room, when the closed container portion of the cap, the simultaneously closed inspection medicament of the cap is kept in
Room so that the inspection medicament temporary room does not connect with the part around the inspection medicament temporary room in the container portion
It is logical；The convex top for being capable of elastic deformation is formed with position in the cap for the closed inspection medicament temporary room,
The convex top is protruded towards the side opposite with the container portion of the cap, and is provided with direction in the convex top
The thorn post that the container portion extends, when the convex top is depressed, the lower end of the thorn post can pierce through the inspection medicament
The bottom of temporary room.
Due to being provided with inspection medicament temporary room in container portion, it is possible to be in advance put into inspection medicament in desinfection chamber
In inspection medicament temporary room, sample to be detected is put into container portion, then by the cap that closes come closed container portion and
Inspection medicament temporary room in container portion.At this point it is possible to closed good whole closed test chamber is placed into common room
Remaining process of the test is carried out in interior environment, it is to avoid after taking desinfection chamber for a long time.
When operator presses some inspection corresponding convex top of medicament temporary room, the lower end of post is pierced by the inspection medicament
Pierce through the bottom of temporary room so that the inspection medicament of storage can be flowed in container portion in the inspection medicament temporary room, so that
Ensure to check medicament to addition in sample in the state of whole closed inspection container closure.Therefore will not be produced in process of the test
Endophytic bacteria is invaded.
Preferably, the bottom of the bottom higher than the container portion of the inspection medicament temporary room so that when the inspection medicine
Inspection medicament of the bottom of agent temporary room when pierced in the inspection medicament temporary room can be flowed in the container.Medicament is temporary
The bottom of the bottom higher than container portion of room is deposited, when the bottom of medicament temporary room is pierced, inspection medicament just can be in gravity
In container portion below all being fallen under effect, and mix with sample.
Preferably, in the state of the convex top is not affected by pressure, the lower end of the thorn post is temporary with the inspection medicament
Depositing has gap between the bottom of room, and the convex top the raised height in the upper surface from the cap more than between described
Gap.There is gap due to piercing between the lower end of post and the bottom of inspection medicament temporary room, so thorn post is not easy because misoperation
And pierce through the bottom of inspection medicament temporary room.Due to pierce post lower end and inspection medicament temporary room bottom between gap with it is convex
There is this magnitude relationship between the height raised from the upper surface of cap on shape top so that convex withstands on and is depressed into and cap
Upper surface it is concordant when thorn post lower end pierced through inspection medicament temporary room bottom so that inspection medicament reliably flow into
To in container portion.
Preferably, the bottom of the inspection medicament temporary room is film.Film is easily pierced, therefore, operator is not required to
Take the bottom being made up of film that too big power can just pierce through inspection medicament temporary room.In addition, film after pierced not
Big retraction is easily produced, puncture mouthful would not also drawn in again due to retraction, therefore, it is possible to ensure that inspection medicament passes through
Mouth is pierced through all to be flowed into container portion.
Preferably, the surface upward of the bottom of the inspection medicament temporary room has water repellency.Inspection medicament leads to
It is often aqueous medicament.Surface with water repellency is the surface contacted with inspection medicament, when the bottom of inspection medicament temporary room
When pierced, inspection medicament without being adhered on this surface with water repellency, but all be flowed into container portion, so that
Ensure that the amount that inspection medicament is put into sample meets desired numerical value.
Brief description of the drawings
Fig. 1 is schematic diagram when air-tight state is according to the closed test chamber of implementation method of the present utility model.
Fig. 2 is schematic diagram when open mode is according to the closed test chamber of implementation method of the present utility model.
Description of reference numerals：
10th, closed test chamber；100th, cap；101st, convex top；102nd, post is pierced；200th, container portion；201st, medicine is checked
Agent temporary room；201a~201c, separate chamber；202nd, thin-walled bottom.
Specific embodiment
Illustrative embodiments of the present utility model are described in detail hereinafter with reference to accompanying drawing.In the following embodiments, it is
The convenience of explanation, shows the example of closed test chamber of the present utility model, but the utility model not limited to this.
Hereinafter, for convenience of description, the mouse chest cell suspension of dilution of predetermined concentration will be adjusted to as this reality
The example of the test samples in mode is applied, will be used to cultivating the IL-1 standard items of mouse chest cell as adding in process of the test
The example of the first inspection medicament in sample, will be used to producing mouse chest cell the 3H-TdR of desired stimulation, POPOP,
The confection of PPP and dimethylbenzene checks showing for medicament as the example of the second inspection medicament using stain for cell as the 3rd
Example.These three inspection medicaments need not being added in sample in the same time in process of the test.It is readily appreciated that, the mesh of these examples
Be only that it is convenient understand technological thought of the present utility model, be not specially limited range of application of the present utility model.
The closed test chamber 10 according to illustrative embodiments of the present utility model is shown in Fig. 1 and Fig. 2.It is closed
Formula test chamber 10 includes container portion 200 and cap 100, and container portion 200 is used to receive sample to be detected, and cap 100 is used for
The opening in closed container portion 200.
Inspection medicament temporary room 201 is formed with inwall at the opening in container portion 200.Medicine is checked in present embodiment
The quantity of agent temporary room 201 is, for example, three, is respectively three separate chambers 201a, 201b and 201c shown in Fig. 2.This implementation
Three separate chambers 201a, 201b and 201c in mode are respectively used to place the first above-mentioned inspection medicament, the second inspection medicine
Agent and the 3rd inspection medicament.
The opening of inspection medicament temporary room 201 is concordant with the opening in container portion 200.When the closed container portion 200 of cap 100
When, cap 100 is simultaneously also closed inspection medicament temporary room 201 (i.e. three separate chambers 201a, 201b and 201c) so that inspection
Medicament temporary room 201 is tested not connected with the part around inspection medicament temporary room 201 in container portion 200.Hereinafter, for side
Just illustrate, the space for not including inspection medicament temporary room 201 in container portion 200 is referred to as sample space, by separate chamber
Space in 201a is referred to as the first inspection medicament space, and the space in separate chamber 201b is referred to as into the second inspection medicament space,
Space in separate chamber 201c is referred to as the 3rd inspection medicament space.The bottom of inspection medicament temporary room 201 is higher than container portion
200 bottom.
The sample that be able to will be made in clean room environment is put into container portion 200, then checks medicine by above-mentioned first
Agent, the second inspection medicament and the 3rd inspection medicament are respectively put into three separate chambers 201a, 201b and 201c, then with lid
Closed container portion 200 of portion 100 so that sample space, the first inspection medicament space, the second inspection medicine are formd in container portion 200
This respective four independent confined space of agent space and the 3rd inspection medicament space.
Being formed with position in cap 100 for closed three separate chambers 201a, 201b and 201c being capable of elasticity
The convex top 101 of deformation.Convex top 101 can be for example formed using the rubber formed as one with cap 100.Convex top
101 protrude towards the side (upside in Fig. 1 and Fig. 2) opposite with container portion 200 of cap 100, and are set in convex top 101
It is equipped with the thorn post 102 extended towards container portion 200.
The lower end for piercing post 102 has sharp shape, when convex top 101 is depressed, pierces the sharp lower end meeting of post 102
Pierce through the thin-walled bottom 202 of corresponding separate chamber 201a, 201b or 201c so that corresponding inspection medicament flows to sample space
In.After the pressing to convex top 101 is unclamped, convex top 101 can return to original in the presence of the elastic restoring force of itself
Rising height.
Thin-walled bottom 202 can be the film for for example being formed by metal foil, in order to be pierced through by thorn post 102.In addition, film
Do not allow to be also easy to produce big retraction after pierced.When the thin-walled bottom 202 of certain separate chamber 201a, 201b and 201c is by thorn post
During 102 puncture, the inspection medicament in the separate chamber can be flowed in sample space from corresponding inspection medicament space.
In the state of convex top 101 is not affected by pressure, the lower end of post 102 and the thin-walled of inspection medicament temporary room 201 are pierced
There is clearance D, the height that the upper surface from cap 100 on convex top 101 is raised is H between bottom 202.H is met between D and H
The relation of ＞ D.So, when convex top 101 is pressed into the height substantially concordant with the upper surface of cap 100, thorn post 102
Thin-walled bottom 202 is just pierced through in lower end so that medicament is flowed into sample space.
In checkout procedure, operator is only needed corresponding separate chamber 201a, 201b or 201c at the predetermined moment
Press on the convex top 101 of top, so that it may so as to need the inspection medicament of addition to be flowed into sample space from medicament space.Meanwhile,
The seal for needing not worry about whole closed inspection container 10 is destroyed, and the bacterium of outside will not be produced to invade closed
Path in inspection container 10.So, using present embodiment closed inspection container 10 tested when, both without it is long when
Between occupy desinfection chamber again will not in sample add inspection medicament when produce bacterial invasion.
In sample space can completely being flowed to for the inspection medicament ensured in each separate chamber 201a, 201b and 201c,
The surface upward of the thin-walled bottom 202 of inspection medicament temporary room 201 has water repellency.For example, being manufactured using by aluminium foil
Thin-walled bottom 202 in the case of, due to aluminium foil material naturally have water repellency, so thin-walled bottom 202 is upward
Surface also have water repellency.Or, can also be coated with the surface upward of thin-walled bottom 202 and for example be formed by wax
Water repellent coating.After operator presses convex top 101 causes that thorn post 102 pierces through thin-walled bottom 202, convex top 101 is in elasticity
Original height of projection is returned in the presence of restoring force, cut is left in thin-walled bottom 202.Due to the court of thin-walled bottom 202
Surface upward has water repellency, so inspection medicament will not be attached to thin-walled bottom 202, but is all flowed from the cut
Enter in sample space.
Although illustrating the utility model with reference to illustrative embodiments, but it is to be understood that the utility model is not
It is limited to disclosed illustrative embodiments.It should be readily apparent to one skilled in the art that on the basis of claims, not carrying on the back
In the case of purport of the present utility model and spirit, various modifications and equivalent can be carried out.For example, inspection medicament is kept in
Room 201 is not limited to three situations, according to needed in process of the test addition inspection medicament species, can be one, two or
Person other quantity.Therefore, the scope of claims should meet broadest explanation, with comprising all of modification, equivalent structure
And function.
Claims (5)
1. a kind of closed test chamber, it includes container portion and cap, and the container portion is used to receive sample to be detected, institute
Cap is stated for the opening in the closed container portion, it is characterised in that
At least one inspection medicament temporary room is formed with inwall at the opening in the container portion, when the cap is closed described
During container portion, the cap simultaneously it is closed it is described inspection medicament temporary room so that the inspection medicament temporary room not with the appearance
The part connection around the inspection medicament temporary room in device portion；
The convex top for being capable of elastic deformation is formed with position in the cap for the closed inspection medicament temporary room,
The convex top is protruded towards the side opposite with the container portion of the cap, and is provided with direction in the convex top
The thorn post that the container portion extends, when the convex top is depressed, the lower end of the thorn post can pierce through the inspection medicament
The bottom of temporary room.
2. closed test chamber according to claim 1, it is characterised in that
The bottom of the bottom higher than the container portion of the inspection medicament temporary room so that when the bottom of the inspection medicament temporary room
Inspection medicament of portion when pierced in the inspection medicament temporary room can be flowed in the container.
3. closed test chamber according to claim 1 and 2, it is characterised in that
In the state of the convex top is not affected by pressure, it is described thorn post lower end with it is described inspection medicament temporary room bottom it
Between there is gap, and the height raised from the upper surface of the cap on the convex top is more than the gap.
4. closed test chamber according to claim 1 and 2, it is characterised in that
The bottom of the inspection medicament temporary room is film.
5. closed test chamber according to claim 4, it is characterised in that
The surface upward of the bottom of the inspection medicament temporary room has water repellency.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621333068.2U CN206253144U (en) | 2016-12-06 | 2016-12-06 | Closed test chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621333068.2U CN206253144U (en) | 2016-12-06 | 2016-12-06 | Closed test chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206253144U true CN206253144U (en) | 2017-06-16 |
Family
ID=59028613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621333068.2U Active CN206253144U (en) | 2016-12-06 | 2016-12-06 | Closed test chamber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206253144U (en) |
-
2016
- 2016-12-06 CN CN201621333068.2U patent/CN206253144U/en active Active
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